key: cord-0755570-85e8bf1g authors: Silva-Boghossian, Carina M.; Dezonne, Romulo S. title: What Are the Clinical and Systemic Results of Periodontitis Treatment in Obese Individuals? date: 2021-08-02 journal: Curr Oral Health Rep DOI: 10.1007/s40496-021-00295-5 sha: 135c24234165fed6aa4b8e5dd10515349f1c5f4f doc_id: 755570 cord_uid: 85e8bf1g PURPOSE OF REVIEW: Periodontitis and obesity are characterized by a dysregulated inflammatory state. Obese individuals have a higher chance of presenting periodontitis. Clinical studies in different populations demonstrate that individuals with obesity have worse periodontal conditions. This current review aims to explore recent literature to understand what the impacts of obesity on periodontal treatment outcomes are and to learn whether periodontal treatment can improve systemic biomarkers in obese individuals. RECENT FINDINGS: Short- and long-term evaluations demonstrated that non-surgical periodontal treatment could improve clinical parameters in obese individuals, represented as the reduction in mean probing depth, sites with probing depth ≥ 4 mm, and extension of bleeding on probing. However, obese individuals may have less clinical improvement when compared to normal-weight individuals with a similar periodontal profile. Additionally, periodontal treatment may contribute to a reduction in systemic levels of retinol-binding protein 4 and leptin, while promoting an increase in systemic levels of adiponectin. SUMMARY: Overall, obese individuals with periodontitis can significantly benefit from non-surgical periodontal treatment. However, clinical improvements seem to be less prominent in obese individuals with periodontitis compared to non-obese individuals with similar periodontal status. Nevertheless, periodontal treatment may impact significantly on the reduction of several biochemical biomarkers of obesity with or without weight reduction. Further investigations are needed to improve our comprehension of the mechanisms underlying those findings. Periodontitis is an inflammatory disease associated with a dysbiotic microbiota [1] . As it is a progressive destructive disease, if left untreated, periodontitis may lead to tooth loss [1] . Advances in periodontal research have demonstrated that periodontal microbiota or their products and local inflammatory mediators can impact a significant number of systemic disorders. Inversely, metabolic diseases can modulate periodontal response to microbial challenge [2] . Two main characteristics of periodontitis, chronic lowgrade inflammation state and potential hematogenous dissemination of bacteria and their endotoxin, such as lipopolysaccharide, may explain the association between periodontal diseases and systemic conditions [2] [3] [4] . Particularly, severe periodontitis cases are at higher risk for cardiovascular disease, acute myocardial infarction, and stroke [5] . Furthermore, the bidirectional relationship between diabetes mellitus and periodontitis is well established, and it is now included in the classification of periodontitis to predict the risk of progression [1] . More recently, the relationship between obesity and periodontitis has been explored, and it also is included in the current classification of periodontal diseases [6, 7] . Hypertrophic or hyperplastic adipose tissue, present in excessive weight gain, can recruit inflammatory cells and induce exacerbated synthesis of pro-inflammatory This article is part of the Topical Collection on Systemic Diseases * Carina M. Silva-Boghossian carinaboghossian@gmail.com adipokines/cytokines [8] [9] [10] . As a consequence, the resulting hyper-inflammatory state could impair periodontal response to bacterial challenge [2], leading to a higher chance of periodontitis in obese individuals [11, 12•, 13, 14, 15••] . Thus, this current review aims to explore recent literature, published within the past 5 years, to understand what the impact of obesity on periodontal treatment outcomes are and to find out whether periodontal treatment can improve systemic biomarkers in obese individuals. Obesity is characterized by an excessive accumulation of body fat [16] . World Health Organization (WHO) and the National Institutes of Health determine obesity by using the body mass index (BMI), obtained as a ratio given by weight (in kg)/height (in m 2 ) that classifies several categories. Thus, individuals who have a BMI ≥ 30.0 kg/m 2 are considered obese and categorized into three degrees, according to their level of obesity: I, 30 .0-34.9 kg/m 2 ; II, 35.0-39.9 kg/m 2 ; III, ≥ 40.0 kg/m 2 [17, 18] . However, BMI is only related to total body weight, and it does not indicate fat distribution or weight composition, whether it is muscular or adipose tissue. In this case, some misinterpretations may occur [19] . For these reasons, other clinical parameters may be relevant to complement the diagnosis of obesity, such as waist circumference, hip circumference, and the calculation of the waist/ hip ratio [20] . This pattern of distribution of body adiposity can predict a greater or less threat to health [21] . At the cellular level, excessive weight gain is observed in adipose tissue by both hyperplasia and hypertrophy of adipocytes [8, 22] , and it is a result of a caloric imbalance and might be originated from a combination of excessive caloric intake and a sedentary lifestyle [23] . Nevertheless, obesity can differ in certain individuals according to their genetic predisposition, as well as environmental changes and epigenetic mechanisms [24] . According to WHO, in 2016, 1.9 billion adults (≥ 18 years of age) were overweight, and more than 650 million exhibited obesity [18] . Prevalence of obesity in the USA ranges from 40 to 45%, according to age group [25] , which is relatively high compared to Sweden (16.6%) [26] and China (ranging from from 1.3 to 12.2% depending on the province) [27] . However, obesity is also frequent in developing countries such as Mexico (36.1%) [28] and India (42%) [29] . Additionally, an increase in obesity rates is reported in developing countries as demonstrated in Brazil, which showed a significant increase in the prevalence of obesity from 2006 (11.8%) to 2019 (20.3%) [30] . That report is an example of an anticipated tendency of constant worldwide growth in the prevalence of obesity [18] , which is expected to worsen as a consequence of the COVID-19 pandemic [31] . Recognizing obesity as a disease is important to tackle public health issues because it usually is associated with comorbidities, and an increased rate of morbidity and mortality [32, 33] . Indeed, obese adult individuals have a higher relative risk of type 2 diabetes mellitus (T2DM) [34] , hypertension [35, 36] , dyslipidemia [37, 38] , and metabolic syndrome [39] . In addition, the incidence of T2DM increases significantly across tertiles of baseline waist circumference, waist-to-hip ratio, and excess visceral fat mass [40] . Abdominal obesity accentuates the problem by the unusually high influx of portal fatty acids and hormones into the liver from omental adipocytes [41] . Evidence suggests that the dysfunction of adipose tissue leads to aberrant production of inflammatory molecules, known as adipokines [9, 42] . In obesity, hypertrophic or hyperplasic white adipocytes recruit several types of inflammatory cells, such as macrophages, T lymphocytes, and mast cells. This inflammatory cell influx leads to an enhancement of pro-inflammatory adipokines/cytokines synthesized in adipose tissue [10, 43, 44] . These adipocytokines act in an endocrine and/or paracrine manner to trigger insulin resistance, endothelial dysfunction, and vascular inflammation [45] . In summary, obesity holds a complex and multifactorial etiology and represents a relevant risk factor for the development of numerous chronic inflammatory pathologies, such as T2DM [34, 46, 47] , cardiovascular diseases [48] [49] [50] [51] , breast cancer [10, [43] [44] [45] [46] [47] [48] [49] [50] [51] [52] , nonalcoholic fatty liver disease [53, 54] , Alzheimer's disease [55] [56] [57] , and periodontitis [58] . Periodontitis is not only associated with periodontal tissue breakdown but also associated with systemic diseases, such as cardiovascular disease and other metabolic diseases [3, 4] . Additionally, individuals with obesity have been identified as having worse periodontal conditions in different populations [11, 12•, 13, 14, 15••] . Women may present greater prevalence of periodontitis and poorer periodontal parameters compared to non-obese women [14, 15••] . However, the association between periodontitis and obesity may not be influenced by gender [59] . Obese patients with periodontitis compared to nonobese with periodontitis may present significantly higher mean periodontal probing depth (PD), probably indicating worse inflammatory clinical aspects and greater challenge for treatment [15 ••] . Furthermore, the link between obesity and periodontitis may start early in life, as demonstrated in a meta-analysis that shows that obese children and adolescents have a higher chance of 1.46 of developing periodontitis [58] . Individuals with obesity present a constant inflammatory state; consequently, it is plausible to imagine that it impacts on the subgingival environment and influences local microbiota. Data from young adults with overweight or obesity without destructive periodontal disease demonstrated higher levels of the pathogenic species Tannerella forsythia and Porphyromonas gingivalis compared to normal-weight individuals with similar periodontal status [60] . Additional analysis of that study demonstrated that obesity parameters, such as waist circumference, hip circumference, and waist-hip ratio, had a significant positive association with P. gingivalis and Treponema denticola. Similar findings were reported in other studies, in which a positive correlation was found between T. forsythia and P. gingivalis and obesity measurements, such as BMI, waist circumference, and waist-hip ratio [61, 62] . Another study, targeting obese women, demonstrated that subgingival microbiota had only a few species differing between obese and non-obese with or without periodontitis [14] . It is worth stressing that those species, in particular P. gingivalis, are considered keystone pathogens in the context of the initiation of a dysbiosis in the periodontium [63] . A higher prevalence of P. gingivalis in obese individuals compared to non-obese individuals may be indicating an initial dysbiotic state [14] . Potentially, it would indicate an increase in the chances of future destructive disease in susceptible individuals [63] . Periodontal bacteria or their endotoxins and inflammatory products can directly or indirectly lead to systemic complications [2]. Frequent bacteremia and systemic spreading of local inflammation, known as metastatic inflammation, occur in patients with periodontitis, potentially impacting pre-existing or causing metabolic disorders [2]. Besides hematogenic dissemination of bacteria, endotoxins, and inflammatory mediators, periodontal bacteria can disseminate through aspiration, i.e., hospitalized patients under artificial ventilation, or ingestion, which can cause inflammatory exacerbation in the lung or gut, respectively [2] . It has been demonstrated that ingested oral bacteria may colonize and persist in the gut [64] , which can alter intestinal immune response, mainly through the accumulation of Th1 cells [65] . Gut microbiota diversity may be impacted by periodontal status [64] . Lourenço et al. [64] demonstrated that different oral species, such as Selenomonas, Leptotrichia, Tannerella, and Campylobacter, in stool samples from healthy or gingivitis/periodontitis patients, presented a positive significant association with bleeding on probing (BOP) and clinical attachment level (CAL). Moreover, experimental studies have demonstrated that animals fed with a high-fat diet and colonized with P. gingivalis may develop glucose intolerance because of the induced inflammation [66] . Periodontitis may also be associated with an impaired incretin axis in obese individuals [67••] . Solini et al. demonstrated that, while insulin levels were similar between obese individuals with and without periodontitis, significantly higher levels of glucoregulatory hormones, glucagon, and glucose-dependent insulinotropic polypeptide (GIP) were found in obese individuals with periodontitis [67••] . Furthermore, individuals with periodontitis and obesity express higher levels of chemerin, an immune-modulatory adipokine molecule, in gingival crevicular fluid compared to non-obese periodontitis patients [68] . Moreover, other proinflammatory cytokines, such as interleukin-6 (IL-6), are as well highly expressed in periodontitis sites from obese compared to non-obese individuals with periodontitis [68] . Other adipokines, such as retinol-binding protein 4 (RBP4) and leptin, have been investigated in gingival crevicular fluid and serum of individuals with obesity and periodontitis [69, 70• 73] . RBP4 induces the production of mediators that regulate the recruitment and adherence of leukocytes; while leptin is a proinflammatory cytokine that can be induced by lipopolysaccharide [69] . Higher levels of RBP4 in gingival crevicular fluid and serum can be found in obese compared to non-obese individuals with periodontal health. However, obese individuals with periodontitis present higher levels of RBP4 compared to non-obese individuals with periodontitis. Interestingly, the same study demonstrated that levels of leptin were higher in the gingival crevicular fluid of periodontally healthy obese and non-obese individuals compared to obese and non-obese individuals with periodontitis [69] . Insulin resistance, commonly observed feature in obese patients, is an initial step and key factor for T2DM and the development of metabolic syndrome [74] . Total circulating adiponectin and high molecular weight adiponectin inversely correlated with adiposity, BMI, glucose, insulin, and triglyceride levels, and visceral fat accumulation; as a consequence, they are decreased in obesity [75] . In this context, a close correlation between hypoadiponectinemia with a decrease in insulin sensitivity and T2DM has also been described in population-based studies [76, 77] . Thus, there is an inverse association between total plasma adiponectin levels and the incidence of T2DM [78, 79] . This correlation is corroborated by the intracellular crosstalk with insulin pathways since adiponectin directly interacts with insulin receptor substrates 1 and 2 (IRS1/2). This binding evokes downstream activation of PI3K, a major component of the insulin pathway [16] , ameliorating the insulin response and triggering anti-inflammatory pathways in peripheral tissues (Fig. 1a ). In individuals with normal insulin sensitivity, insulin activates two different paths. The first one is an antiinflammatory, anti-apoptotic, and anti-oxidative path mediated by IRS activation. In this case, adiponectin acts as a coactivator of IRS response enhancing insulin sensitivity and a strong anti-inflammatory molecule by inhibiting nuclear factor kappa-B (NFκ-B) response [80] . The secondary branch of the insulin pathway is MAPK/ERK activation related to cellular proliferation and growth that evokes a pro-inflammatory phenotype. However, in non-obese individuals, these two pathways are balanced (Fig. 1a ) [81] . Insulin signaling is mediated by its receptor (InsR), in the cell membrane, which triggers two different intracellular pathways. The first one is called the metabolic arm; this path is dependent on IRS 1/2 and downstream activation of the PI3K-AKT path. This metabolic arm, besides its effects on glucose and lipid metabolism, possesses anti-apoptotic, anti-oxidative stress, and anti-inflammation response. The second arm (mitogenic arm) is mediated by MAPK-ERK activation, enhancing cellular growth, proliferation, and hypertrophy and evoking a pro-inflammatory response. In addition, insulin also induces FOXO1 transcription factor phosphorylation, preventing its nuclear translocation and AdipoRs transcriptional repression. Adiponectin is an insulin sensitize molecule that triggers the PI3K path through IRS 1/2 activation by its downstream effector APPL1. Moreover, adiponectin inhibits NFκ-B response, increases fatty acid oxidation, and decreases intracellular ceramide preventing endoplasmic reticulum (ER) stress. b Insulin pathways in insulin-resistant patients. The first step during insulin resistance is downregulation of circulating adiponectin that induces a pro-inflammatory response, mediated by NFκ-B, and reduces activation of PI3K-AKT, the metabolic arm path. In this case, downregulation of the anti-inflammatory metabolic arm of the insulin path favors the activation of the mitogenic and pro-inflammatory arm. In turn, downregulation results in nuclear translocation of the unphosphorylated form of the FOXO1 transcription factor that represses the transcription of adiponectin receptors. The major reduction in the adiponectin pathway increases the intracellular level of ceramides, which in turn lead to endoplas-mic reticulum (ER) stress. The basal pro-inflammatory status of visceral adipose tissue enhances both TNF-α and IL-6 activation. TNF-α triggers JNK and IKK activation, part of the upstream NF-κ-B path, blocking IRS1/2 signal. The IL-6 signal transduction cascade activation induces SOCS3 transcription that also inhibits IRS1/2 activation. Taken together, these events drastically inhibit the metabolic anti-inflammatory arm of the insulin path decreasing both insulin and adiponectin sensibility in peripheral tissues favoring the pro-inflammatory response. ACC acetyl carboxylase, ACO enzyme acyl-CoA oxidase, AdipoRs and AdipoR1/2 adiponectin receptor 1 and 2, AKT or PKB protein kinase B, AMPK AMP-activated protein kinase, AP-1 activator protein 1, APPL1/2 an adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 and 2, ERK extracellular signal-regulated kinases, FOXO1 Forkhead Box O1 (FOXO1) transcription factor, Gbr2 growth factor receptor-bound protein 2, IKK inhibitor of nuclear factor kappa-B kinase, IL-6 interleukin-6, InsR insulin receptor, IRS 1/2 insulin receptor substrate 1 and 2, JAK Janus kinase, JNK c-Jun N-terminal kinases, MAPK mitogenactivated protein kinase, MEK mitogen-activated protein kinase and MAP2K, NFκ-B nuclear factor kappa beta, p38MAPK p38 mitogenactivated protein kinase, PDK1 3-phosphoinositide-dependent protein kinase-1, PGC1-α peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PI3K phosphoinositide 3-kinase, PPAR-α peroxisome proliferator-activated receptor-alpha, RAF RAF proto-oncogene serine/threonine-protein kinase, RAS rat Sarcoma virus, SOCS3 suppressor of cytokine signaling 3, STAT3 signal transducer and activator of transcription protein 3, TNF-α tumor necrosis factor-alpha On the other hand, in insulin and adiponectin-resistant patients, the inflammatory status of adipose tissue disrupts the IRS arm of insulin pathways, favoring a pro-inflammatory phenotype. Initially, low circulating adiponectin results in a downregulation of the metabolic arm (IRS path) and nuclear translocation of unphosphorylated FOXO1, which, in turn, reduces adiponectin receptor synthesis and membrane translocation [82] . The downregulation of the adiponectin pathway results in oxidative stress and activation of NFκ-B (Fig. 1b ) [16] . In addition, the low-grade inflammation observed in obese individuals is a result of augmented expression of inflammatory cytokines, by visceral adipose tissue [9] . Among those molecules expressed and synthesized by this dysfunctional tissue are tumor necrosis factor-alpha (TNFα) and IL-6. In obese individuals, these pro-inflammatory adipokines act in an endocrine and/or paracrine manner to trigger insulin resistance, endothelial dysfunction, and vascular inflammation by inhibiting the IRS branch of the insulin pathway in peripheral tissues (Fig. 1b) , favoring a pro-inflammatory response [10, 45] . Taken together, these molecular responses might be responsible for enhancing local inflammation, such as periodontitis, by inducing an insulin and adiponectin resistant status and elevating systemic inflammation. Interventional studies have investigated whether obesity may impair the outcomes of periodontitis treatment. A systematic review, which included eight studies, investigated the impact of obesity in the reduction of PD after non-surgical periodontal treatment [83] . Three reports included in that review demonstrated that obesity may not interfere with the clinical outcomes, while other five studies found obesity negatively influencing the reduction of PD after periodontal treatment, especially when moderate and severe PD were present before treatment. One of those studies found that the negative impact of obesity on clinical periodontal parameters can be compared to those of the smoking habit. However, another systematic review demonstrated that obesity does not impact periodontitis treatment [59] . More recently, other studies with obese and non-obese individuals demonstrated that obesity status does not impact clinical periodontal outcomes after non-surgical therapy [ Balli et al. [68] Aim: to investigate how the obesity/periodontitis and non-surgical periodontal treatment affect GCF chemerin levels in periodontitis and to understand how chemerin may be utilized as a potential diagnostic/prognostic biological indicator for periodontal disease to discern the relation between chemerin and IL-6, a highly functional, proinflammatory adipocytokine Wanichkittikul et al. [73] Aim to: -investigate changes in serum leptin, adiponectin, and CRP levels after non-surgical periodontal treatment in Thai patients with overweight or obesity who did or did not exhibit severe periodontitis, compared with normal-weight patients with or without severe periodontitis Table 1 . Another short period evaluation also demonstrated a lesser improvement in the extension of BOP in obese compared to non-obese individuals with periodontitis [86] . In a 6-month evaluation after treatment, Suvan et al. [87••] were also able to demonstrate that obese patients had significantly less improvement in periodontal parameters after therapy compared to lean individuals. Those differences were detected in the final percent of PD > 4 mm, percent of PD > 5 mm, and percent of fullmouth bleeding (Table 1) . Another study by Suvan et al. [15••] demonstrated at 6 months that, although a significant reduction in mean PD and percent of BOP is detected in comparison with baseline, those parameters were significantly higher in obese compared to non-obese with similar mean CAL and percent of BOP in the baseline (Table 1) . The impact of weight loss has also been evaluated concerning periodontal status after bariatric surgery or dietary therapy [ Treatment of periodontitis associated with dietary therapy was investigated by Martinez-Herrera et al. [72••] who demonstrated that obese individuals that lose weight along with periodontal treatment have a significantly higher reduction in mean PD and percentage of sites with moderate PD (4 to 5 mm) than obese individuals with periodontitis without weight loss (Table 1) . However, when researchers adjusted their data for complement 4 and TNF-α, respectively, those differences were no longer significant. Another study by Martinez-Herrera et al. [71••] confirmed that, demonstrating that obese individuals on low-calorie diet compared to a group without diet had similar clinical periodontal outcomes after non-surgical periodontal treatment (Table 1) . Another question investigated is whether periodontal treatment can impact obesity biomarkers, which could result in an improvement in systemic health. Balli et al. [68] demonstrated that non-surgical periodontal treatment can lead to a decrease in the expression of chemerin in gingival crevicular fluid in obese individuals with periodontitis. Even though it was tested locally, it is potentially indicating that systemic levels of that adipokine might be reduced after periodontal therapy. However, other investigations showed that periodontal treatment was not efficient in reducing serum levels of resistin in obese and non-obese individuals [86] . On the other hand, Suvan et al. [15••] demonstrated that obese individuals can significantly reduce their systemic levels of glucagon after periodontal treatment, becoming similar to the ones of non-obese individuals (Table 2) . Periodontitis treatment can also lead to a reduction in systemic levels of leptin, and C-reactive protein, especially in patients with severe periodontitis [73, 85] . Periodontal therapy can also contribute to a significant increase in systemic levels of adiponectin [73] . Moreover, an improvement in the lipid profile of obese individuals may also be a consequence of the treatment of periodontitis [85] (Table 2) . Further analysis of Martinez-Herrera et al. [71••] investigated oxidative stress in leukocytes and leukocyte-endothelial cell interactions after treatment of periodontitis in obese individuals with or without dietary treatment. It was interesting to observe that both groups, with or without low calories diet, had their serum levels of RBP4 and TNF-α significantly decreased after periodontal treatment. Moreover, another striking piece of data is that periodontal treatment alone was able to reduce total superoxide and intracellular calcium as found in without diet group. It is important to highlight that finding because it may be indicating that periodontal treatment may help improve the systemic conditions of obese individuals under weight-loss therapy. Another study by Martinez-Herrera et al. [70 ••] compared non-obese and obese individuals regarding serum levels of many biochemical parameters after treatment of periodontitis (Table 2) . Once again, the authors were able to demonstrate that periodontal treatment alone can significantly reduce serum levels of RBP4 and TNF-α in 12 weeks of observation. The effect of dietary therapy and periodontal treatment was also investigated by the same group on systemic parameters of inflammation [72••] . It was observed that levels of RBP4 reduce after therapy in both, regardless of the diet. However, a significant decrease in levels of TNF-α and C3 was observed after periodontal therapy only for the obese individuals going on a low calory diet (Table 2) . Recent literature demonstrated that obese individuals with periodontitis benefit from non-surgical periodontal treatment. However, periodontal therapy can result in inferior clinical improvements in obese individuals compared to non-obese ones. Nevertheless, available evidence demonstrated that periodontal treatment significantly reduces Table 1 Studied parameters: -Systemic levels of RBP4 -Systemic levels of TNF-α -Oxidative stress of leucocytes (total ROS production, superoxide production, levels of cytosolic Ca 2+ , mitochondrial membrane potential) -Antioxidant status -Leucocytes and endothelial cells interaction Obese without diet: 4.4 ± 0.9 Obese with diet: 3.7 ± 1.1 12 weeks Obese without diet: 3.9 ± 1.1* Obese with diet: 3.3 ± 0.9* Levels of TNF-α Baseline Obese without diet: 17.8 ± 3.1 Obese with diet: 17.1 ± 6.2 12 weeks Obese without diet: 16.5 ± 4.1 Obese with diet: 13.0 ± 1.8* * intragroup analysis, a significant difference compared to baseline After treatment, both groups had a significant reduction in: -Total superoxide -Intracellular calcium Martinez-Herrera et al. [72••] Additional information on Table 1 Studied parameters: -glucose -insulin -HOMA-IR -TC -HDL-C -LDL-C -TG -TNF-α -IL-6 -hs-CRP -C3 -RBP4 Table 1 Studied parameters: -glucose -insulin -HOMA-IR -TC -HDL-C -LDL-C -TG -TNF-α -IL-6 -hs-CRP -RBP4 Baseline Non-obese: 3.2 ± 0.6 Obese: 3.8 ± 1.0 12 weeks Non-obese: 3.0 ± 0.7* Obese: 3.4 ± 1.0* Levels of TNF-α Baseline Non-obese: 10.8 ± 3.8 Obese: 17.2 ± 9.8 12 weeks Non-obese: 9.6 ± 4.9* Obese: 13.9 ± 5.3* * intragroup analysis, a significant difference compared to baseline No other parameter showed a significant difference after periodontal treatment in both groups several biochemical biomarkers of obesity with or without weight reduction. Further investigations are needed to improve our comprehension of mechanisms that can explain that mechanism. Despite controversies in clinical findings after periodontal therapy in obese individuals, dental professionals should be aware that obesity is a chronic metabolic disease, and that periodontal treatment should be a part of a comprehensive treatment of obesity. It is reasonable to propose that the management of periodontitis in obese individuals should require the interaction between dental professionals and other health care providers, as physicians, nutritionists, and physical educators. On the other hand, obese individuals should be referred for periodontal prevention and treatment not only to promote improvement in systemic inflammatory status but also in the quality of life. The authors declare no competing interests. This article does not contain any studies with human or animal subjects performed by any of the authors. Scientific evidence on the links between periodontal diseases and diabetes: consensus report and guidelines of the joint workshop on periodontal diseases and diabetes by the International Diabetes Federation and the European Federation of Periodontology Periodontitis and cardiovascular diseases Inflammation of the periodontium associates with risk of future cardiovascular events Manifestations of systemic diseases and conditions that affect the periodontal attachment apparatus: case definitions and diagnostic considerations Periodontal manifestations of systemic diseases and developmental and acquired conditions: consensus report of workgroup 3 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions The adipose tissue microenvironment regulates depotspecific adipogenesis in obesity Adipokine dysregulation and adipose tissue inflammation in human obesity Adipocyte ceramides regulate subcutaneous adipose browning, inflammation, and metabolism Waist circumference and waist-to-height ratio are associated with periodontal pocketing-results of the Health The results of this systematic review demonstrated that obesity indicators are significantly associated with periodon Body fat rather than body mass index is associated with gingivitis-a southern Brazilian cross-sectional study Subgingival microbial profile of obese women with periodontal disease Effect of treatment of periodontitis on incretin axis in obese and nonobese individuals: a cohort study Insights into the controversial aspects of adiponectin in cardiometabolic disorders In BMI we trust: reframing the body mass index as a measure of health Obesity and type 2 diabetes: two diseases with a need for combined treatment strategies-EASO can lead the way A composite of BMI and waist circumference may be a better obesity metric in Indians with high risk for type 2 diabetes: an analysis of NMB-2017, a nationwide cross-sectional study Obesity is a risk factor for developing critical condition in COVID-19 patients: a systematic review and meta-analysis Oxidative stress resulting from the removal of endogenous catalase induces obesity by promoting hyperplasia and hypertrophy of white adipocytes Screen time, other sedentary behaviours, and obesity risk in adults: a review of reviews Molecular genetics of human obesity: a comprehensive review Prevalence of obesity and severe obesity among adults: United States Prevalence and time trends of overweight, obesity and severe obesity in 447,925 Swedish adults Prevalence of overweight and obesity in China: results from a crosssectional study of 441 thousand adults Obesity prevalence and determinants among young adults, with special focus on normal-weight obesity; a cross-sectional study in Mumbai Temporal trend of overweight and obesity prevalence among Brazilian adults, according to sociodemographic characteristics The escalating global burden of obesity following the COVID-19 times-are we ready? Health and economic burden of the projected obesity trends in the USA and the UK Body mass index and all-cause mortality in heart failure patients with normal and reduced ventricular ejection fraction: a dose-response meta-analysis Body weight considerations in the management of type 2 diabetes Obesity, hypertension and diuretic use as risk factors for incident gout: a systematic review and meta-analysis of cohort studies Effects of blood pressure lowering agents on cardiovascular outcomes in weight excess patients: a systematic review and meta-analysis Dyslipidemia and meibomian gland dysfunction: utility of lipidomics and experimental prospects with a diet-induced obesity mouse model Secondary dyslipidemia in obese children-is there evidence for pharmacological treatment? COSMIC project: consensus on the objectives of the metabolic syndrome in clinic Dysfunctional adiposity and the risk of prediabetes and type 2 diabetes in obese adults Branched short-chain fatty acids modulate glucose and lipid metabolism in primary adipocytes Adipocytokines: mediators linking adipose tissue, inflammation and immunity Metabolic endotoxemia promotes adipose dysfunction and inflammation in human obesity Adipose tissue inflammation in breast cancer survivors: effects of a 16-week combined aerobic and resistance exercise training intervention Adipokines and inflammatory markers in elderly subjects with high risk of type 2 diabetes and cardiovascular disease Comparison of serum high-molecular weight (HMW) adiponectin with total adiponectin concentrations in type 2 diabetic patients with coronary artery disease using a novel enzyme-linked immunosorbent assay to detect HMW adiponectin Metabolic and clinical outcomes in nondiabetic individuals with the metabolic syndrome assigned to chlorthalidone, amlodipine, or lisinopril as initial treatment for hypertension: a report from the Antihypertensive and Lipid-Lowering Treatment to Prevent Heart Attack Trial (ALLHAT) Adiponectin, a therapeutic target for obesity, diabetes, and endothelial dysfunction Baseline adiponectin concentration and clinical outcomes among patients with diabetes and recent acute coronary syndrome in the EXAMINE trial Obesity-induced hypertension: brain signaling pathways The adiponectin paradox for all-cause and cardiovascular mortality Associations of insulin resistance and adiponectin with mortality in women with breast cancer Insulin/ Snail1 axis ameliorates fatty liver disease by epigenetically suppressing lipogenesis Global epidemiology of nonalcoholic fatty liver disease-meta-analytic assessment of prevalence, incidence, and outcomes APOE and cholesterol homeostasis in Alzheimer's disease Meta-analysis of Alzheimer's disease risk with obesity, diabetes, and related disorders Adiponectin paradox in Alzheimer's disease; relevance to amyloidogenic evolvability? Front Endocrinol (Lausanne) Association between overweight/obesity and periodontal disease in children and adolescents: a systematic review and meta-analysis Association between obesity and periodontal disease. A systematic review of epidemiological studies and controlled clinical trials Subgingival microbiota in overweight and obese young adults with no destructive periodontal disease Does obesity influence the subgingival microbiota composition in periodontal health and disease? Comparative analysis of subgingival red complex bacteria in obese and normal weight subjects with and without chronic periodontitis Periodontal inflammation: integrating genes and dysbiosis Defining the gut microbiota in individuals with periodontal diseases: an exploratory study Ectopic colonization of oral bacteria in the intestine drives TH1 cell induction and inflammation Periodontitis induced by Porphyromonas gingivalis drives periodontal microbiota dysbiosis and insulin resistance via an impaired adaptive immune response Periodontitis affects glucoregulatory hormones in severely obese individuals Chemerin and interleukin-6 levels in obese individuals following periodontal treatment Correlation of crevicular fluid and serum levels of retinol-binding protein 4 and leptin in chronic periodontitis and obesity Levels of serum retinol-binding protein 4 before and after non-surgical periodontal treatment in lean and obese subjects: an interventional study Effect of non-surgical periodontal treatment on oxidative stress markers in leukocytes and their interaction with the endothelium in obese subjects with periodontitis: a pilot study Dietary therapy and non-surgical periodontal treatment in obese patients with chronic periodontitis Periodontal treatment improves serum levels of leptin, adiponectin, and C-reactive protein in Thai patients with overweight or obesity Associations of different adipose tissue depots with insulin resistance: a systematic review and meta-analysis of observational studies Adiponectin oligomers as potential indicators of adipose tissue improvement in obese subjects Adiponectin and the incidence of type 2 diabetes in Hispanics and African Americans: the IRAS Family Study Vascular effects of adiponectin: molecular mechanisms and potential therapeutic intervention Prospective study of sexspecific adiponectin changes and incident metabolic syndrome: the ARIRANG study Plasma adiponectin levels and type 2 diabetes risk: a nested case-control study in a Chinese population and an updated meta-analysis Adiponectin affects lung epithelial A549 cell viability counteracting TNFalpha and IL-1ss toxicity through AdipoR1 Increase in PPARgamma inhibitory phosphorylation by Fetuin-A through the activation of Ras-MEK-ERK pathway causes insulin resistance Transcriptional analysis of FOXO1, C/EBP-alpha and PPAR-gamma2 genes and their association with obesity-related insulin resistance Influence of obesity on the outcome of non-surgical periodontal therapy -a systematic review Clinical and microbiological evaluation of nonsurgical periodontal therapy in obese and non-obese individuals with periodontitis: a 9-month prospective longitudinal study Lipid profile and high-sensitivity C-reactive protein levels in obese and non-obese subjects undergoing non-surgical periodontal therapy Impact of non-surgical periodontal therapy on serum resistin and periodontal pathogen in periodontitis patients with obesity Obesity as predictive factor of periodontal therapy clinical outcomes: a cohort study Clinical periodontal conditions in individuals after bariatric surgery: a systematic review and meta-analysis Methods ResultsMd Tahir et al. [86] Additional information on Table 1 Parameter: -Serum resistin Non-obese: 6.9 (4.3, 9.