key: cord-0757758-af88ubdl
authors: Dufour, Catherine; Co, Thien-Kim; Liu, Antonio
title: GM1 Ganglioside Antibody and COVID-19 related Guillain Barre Syndrome – a Case Report, Systemic Review and Implication for Vaccine Development
date: 2021-01-13
journal: Brain Behav Immun Health
DOI: 10.1016/j.bbih.2021.100203
sha: 73461085ade13a8c1b45048b22e093957ebd01b7
doc_id: 757758
cord_uid: af88ubdl

BACKGROUND: Guillain Barre Syndrome (GBS) and Miller Fisher Syndrome (MFS) are emerging as known consequences of COVID-19 infection. However, there have been no reported cases with positive GM1 or GQ1b antibodies in the literature to date. Although clinically similar, the pathophysiology of COVID-19 related GBS and MFS may be significantly different from cases in the pre-pandemic era. CASE PRESENTATION: We present a patient with ascending areflexic weakness consistent with GBS with positive GM1 antibody. The patient had recovered from COVID-19 infection two weeks prior with mild viral illness and symptoms. Her weakness was isolated to the lower extremities and improved after intravenous immunoglobulin treatment. Patient recovered eventually. CONCLUSIONS: – The general lack of reported ganglioside antibodies supports a novel target(s) for molecular mimicry as the underlying etiology, which raises the concern for possible vaccine induced complication. Whether the current GM1 positive case is a sequalae of COVID-19 or a mere coincidence is inconclusive. Further understanding of the disease mechanism of pandemic era GBS and MFS, including antigen target(s) of COVID-19, may be of utmost importance to the development of a safe COVID-19 vaccine.

GD1a, GD1b, and GQ1b. GM1 is the most common ganglioside antibody found in patients with GBS while GQ1b is associated with MFV. GM1 is found to be positive in 88% of GBS patients in one study [2] . Prior to the current COVID-19 pandemic, it was uncommon to have a completely negative ganglioside antibody panel for cases of GBS and MFS. Aside from our current case, a review of available articles yields no reported cases of COVID-19 related GBS or MFS which tested positive for GM1 or GQ1b. The results of our review are presented, followed by discussion of possible pathophysiology and important implications.

A Pub Med literature search was performed on August 28, 2020 which yielded 102 entries related to COVID-19 induced GBS and/or MFV. Searches were performed cross referencing the following key terms "Covid-19", "Guillain Barre", "Miller Fisher", "GM1", "GQ1b", and "Ganglioside". Of the 102 articles found eight were large scale review papers [3] [4] [5] [6] [7] [8] [9] [10] ; the other entries were all case reports. All cases included in the eight review papers and each separate case studies were reviewed. All cases in the review papers were tracked to their primary source to prevent duplication of cases. Laboratory data and ganglioside antibody testing was extracted from the case descriptions. Ganglioside antibody testing was either not reported (NR) or the results were clearly stated. We then included the ganglioside antibody testing information on our case.

A 36-year-old woman with obesity presented to Emergency Department (ED) with 3 days of progressive ascending weakness. On day of admission, she was unable to stand and ambulate. Her history is significant for recent recovery from a COVID-19 infection. She was exposed to the virus by her brother. She had a positive nasopharyngeal swab, PCR SARS-CoV-2 testing, 3 weeks prior to presenting to the ED. She experienced loss of smell and taste, generalized malaise and mild shortness of breath. She was released after one day of hospitalization and recuperated at home. She did not receive remdesivir or steroid. She never had muscle weakness or gait difficulty until 3 days prior to the current admission. On examination, she had normal vital signs. General overall examination by the ED was unremarkable. On neurological examination, she was oriented and coherent. Cranial Nerves 1-12 were all intact. Upper extremities strength was 5/5 and lower extremities strength was 3/5. There were no sensory deficits. She had absent deep tendon reflexes. The patient did not have bowel or bladder control problems. She was unable to stand or ambulate at the time of admission. Her upper extremities demonstrated no dysmetria or dysdiadochokinesia. MRI of head and whole spine were negative for demyelination, or infarct. Her blood chemistry, hematology studies, and hepatic function tests were all normal. Her COVID-19 nasopharyngeal swab test was negative at the time of current presentation. Spinal fluid analysis showed 0 WBC, 0 RBC, and protein of 20 mg/dL. Her B12 level was normal at 499 pg/mL. Ganglioside antibody panel was positive for multiple antibodies (see table) . Patient deferred electrodiagnostic studies.

The patient's lower extremities strength continued to worsen for 3 days in the hospital. Intrvenous immunoglobulin at 400 mg/kg/day was administered for 5 days. After one week of hospitalization, her strength began to improve. She was eventually discharged home after 10 days in the hospital. A follow up phone call after 3 weeks, found that that patient was already ambulating with a walker and even short distances with a cane.

The most recent review paper by Abu-Rumeileh et al is also the most extensive, which included 73 cases [3] . The case with positive GM2 testing reported by Chan was mistakenly noted as GM1 in the review paper by Abu-Rumeileh et al [3] . In over half of the cases reported, cerebral spinal fluid analysis was not performed or mentioned. Based on our review, many of the cases lack electrodiagnostic studies. In the case reported with positive GD1a testing, COVID-19 testing was negative and the patient was asymptomatic. COVID-19 diagnosis was made based on the appearance of "ground glass" on the patient's chest CT scan. In Lantos' case, the Asialo GM1 was only equivocally positive. Our review shows there is roughly 14% positivity on ganglioside antibody studies in presumed COVID-19 related GBS and MFS cases. There have been no cases positive for GM1 in those with GBS and no MFS cases with positive GQ1b testing prior to our case.

The patient in our case has clinical presentation, laboratory studies and therapeutic response typical of GBS. Neurophysiological studies usually play an important role in the diagnosis, subtype determination and prognosis estimation. Lacking such is a potential weakness of our case. However, with our current pandemic, such studies are lacking in more than half of the reported cases due to various reasons and availability. With a positive GM1 antibody test, we are confident that GBS is our patient's diagnosis.

Although Since COVID related GBS and MFS cases do respond favorably to IVIG and plasma exchange, molecular mimicry is probably the most likely etiology. Due to the absence of cases reporting GM1 and GQ1b positive testing prior, it appears that COVID-19 induces GBS and MFS through a different target(s). There are many articles citing the pathologic role played by angiotensinconverting enzyme 2 (ACE2) receptor. ACE2 is present on neuron and glial cells [22] . Other candidate being mentioned is heat shock protein (HSP), in particular HSP 27, 60, 70 and 90 [23] . Identifying the true antigen related to COVID-19 induced GBS and MFS may be of paramount importance to improving the safety profile of these new vaccines. This concern is not unfounded as there was a significant increase of GBS cases after the 1976 swine flu vaccine program [24] . Current vaccine production usually takes an estimated 10-15 years from concept to mass production. This consists of three phases that demonstrate the safety, immunogenicity, and efficacy in a large-scale population. We want to ensure that traditional safeguards are not bypassed to expedite the release of a COVID-19 vaccine [25] .

is likely still occurring at their usual rates. We have seen a slight decrease in cases recently but this is likely due to social distancing and increased hand hygiene which reduces exposure to the usual germs or viruses that lead to GBS and MFS. Due to the overwhelming majority of negative GM1 or GQ1b in COVID-19 associated GBS and MFS seen so far, our case here can be interpreted as either the very first documented case of GM1 positive COVID-19 related GBS or the first confirmed non-COVID-19 related GBS. It is likely that any further report citing positive GM1 or GQ1b testing maybe a coincidence rather than a true association.

More effort and research are needed to locate the true antigen target(s) in COVID-19 related GBS and MFS. This may be essential in determining the true incidence of COVID-19 related GBS and MFS, as well as in the development of more effective treatment and ultimately a safe vaccine.

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The authors would like to thank Drs. Mark Miller, Ann Tan, Juan Barrio and Byron Williams for their opinion and support. We would also like to thank Myrna Thompson for making these tests possible.