key: cord-0791876-yymudgzs
authors: Barbui, Tiziano; De Stefano, Valerio
title: Philadelphia-Negative Myeloproliferative Neoplasms Around the COVID-19 Pandemic
date: 2021-09-29
journal: Curr Hematol Malig Rep
DOI: 10.1007/s11899-021-00647-z
sha: d31d412a382c51fc4958bc181772ad2f8c164e23
doc_id: 791876
cord_uid: yymudgzs

PURPOSE OF REVIEW: Coronavirus disease 2019 (COVID-19) is associated with a high rate of respiratory failure, thromboembolism, bleeding, and death. Patients with myeloproliferative neoplasms (MPNs) are prone to both thrombosis and bleeding, calling for special care during COVID-19. We reviewed the clinical features of MPN patients with COVID-19, suggesting guidance for treatment. RECENT FINDINGS: One study by the European LeukemiaNet collected 175 MPN patients with COVID-19 during the first wave of the pandemic, from February to May 2020. Patients with primary myelofibrosis (PMF) were at higher risk of mortality (48%) in comparison with essential thrombocythemia (ET) (25%) and polycythemia vera (19%); the risk of death was higher in those patients who abruptly discontinued ruxolitinib. In patients followed at home, in regular wards, or in ICU, the thrombosis rate was 1.0%, 2.8%, and 18.4%, respectively. Independent risk factors for thrombosis were ET phenotype, transfer to ICU, and neutrophil/lymphocyte ratio; major bleeding occurred in 4.3% of patients, particularly those with PMF. SUMMARY: MPN patients with non-severe COVID-19 treated at home should continue their primary or secondary antithrombotic prophylaxis with aspirin or oral anticoagulants. In the case of hospitalization, patients assuming aspirin should add low molecular weight heparin (LMWH) at standard doses. In contrast, LMWH at intermediate/therapeutic doses should replace oral anticoagulants prescribed for atrial fibrillation or previous venous thromboembolism. Intermediate/high doses of LMWH can also be considered in ICU patients with ET, particularly in the case of a rapid decline in the number of platelets and progressive respiratory failure.

The SARS-CoV-2 coronavirus infection causing the coronavirus disease 2019 (COVID-19) is a highly contagious and life-threatening disease. No country, race, ethnicity, or religion is spared from this infection (https:// coron avirus. jhu. edu/ map. html). All ages are susceptible to COVID-19, and the clinical spectrum appears to be broad. The features of severe COVID-19 are dominated by an acute pneumonic process with extensive radiologic opacity and, on autopsy, diffuse alveolar damage, inflammatory infiltrates, and microvascular thrombosis. Moreover, infected patients are also likely to be at increased risk of venous and arterial thromboembolism, as reported in China [1] and confirmed in autopsies of patients experiencing sudden death [2] . Both elderly and young patients can succumb to death depending on their underlying health conditions such as cardiovascular diseases, kidney damage, liver dysfunction, diabetes, and cancer [3] .

The risk of getting SARS-CoV-2 infection may vary according to the individual MPN subtype. Only one study by the European LeukemiaNet (ELN) investigators reported the clinical epidemiology of COVID-19 in patients with essential thrombocythemia (ET), polycythemia vera (PV), prefibrotic myelofibrosis (pre-PMF), and primary myelofibrosis (PMF) [4••] . One hundred seventy-five patients with COVID-19 were collected in 38 international hematologic centers during the first wave of the pandemic, from February to May 2020. Among the MPN phenotypes, patients with PMF, including pre-PMF and overt PMF, were the great majority, accounting for 44% of those reported. This is not surprising due to the natural propensity to get infections in PMF. In a large population-based matched cohort study carried out in Sweden, including 8363 MPN patients and 32,405 controls using high-quality registers, the hazard ratio (HR) of any infection in MPN was 2.0 (bacterial infections HR 1.9, 95% CI 1.8-2.0 and viral infections HR 2.1, 95% CI 1.9-2.3). Notably, the HR of any infection was highest in PMF (HR 3.7, 95% CI 3.2-4.1) and significantly elevated in all MPN subtypes (HR 1.7, 95% CI 1.6-1.8 in PV and HR 1.7, 95% CI 1.5-1.8 in ET) [5••] .

Many studies have been undertaken regarding hematologic malignancies addressing numerous issues either related to the infection and the underlying hematologic cancer itself. In a recent systematic review and meta-analysis, fatalities were calculated to occur in 34% of hospitalized adult patients [6•] . Advanced age (≥ 60 years) and non-white race were identified as risk factors for death. Mortality rate varied on the basis of the type of malignancy: 53% of patients with acquired bone marrow failure syndromes, 41% of patients with acute leukemias, 33% of patients with plasma cell dyscrasias, 32% of patients with lymphomas, 31% of patients with chronic lymphocytic leukemia, and 34% of patients with myeloproliferative neoplasms (MPNs) [6• ]. These estimates are three-fold higher than those reported in hospitalized patients with COVID-19 of the general population in Europe and the USA [7] [8] [9] . When compared with the general population with COVID-19, the mortality ratio in the ELN study [4••] was at least two to three times higher than the mortality rates reported by Johns Hopkins in the same period of the first wave of the pandemic and comparable to that reported in other hematologic malignancies collected in the same period [6 •, 10••].

In univariate analysis, age, male gender, decreased lymphocyte counts, need for respiratory support, comorbidities, and diagnosis of PMF showed a significant relationship with death. Patients with MF were at higher risk of mortality (48%) in comparison with ET (25%) and PV (19%). No correlation between the driver mutation genotype and death was found. Regarding MPN-directed therapy ongoing at COVID-19 diagnosis, hydroxyurea (HU) did not show significant correlations. In contrast, multivariable and propensity score matching analyses found an increased risk of death in patients who abruptly discontinued ruxolitinib treatment [4••] . According to the recommendations of the American Society of Hematology (https:// www. hemat ology. org/ covid-19/ covid-19-and-myelo proli ferat ivene oplas msi), if ruxolitinib needs to be stopped, it should be tapered cautiously (https:// www. hemat ology. org/ covid-19/ covid-19-and-myelo proli ferat ivene oplas ms).

To optimize the management of MPN patients with COVID-19, the ASH experts are maintaining a webpage to assist hematologists in navigating the COVID-19 public health crisis.

MPN patients are predisposed to thrombosis and bleeding, and these events contribute considerably to morbidity and mortality. Thrombosis can be the first presenting sign preceding the diagnosis of MPN in 20% of cases, with a persistent risk during the follow-up, where the incidence is the highest in patients with PV (3.5 per 100 patient-years) and similar in ET and PMF (2.5 per 100 patient-years). Particularly in ET, arterial events are more prevalent (70%) than venous thromboembolic events (VTE), which encompass deep vein thrombosis of the leg or pulmonary embolism and uncommon sites such as splanchnic or cerebral veins [11] .

In the most extensive epidemiologic study in PV (i.e., the European Collaboration on Low-dose Aspirin [ECLAP] study), cardiovascular mortality accounted for 45% of all deaths (1.7 deaths per 100 persons per year, with a cumulative incidence of 4.5%), mainly due to coronary heart disease (15% of all deaths), congestive heart failure (8%), non-hemorrhagic stroke (8%), and pulmonary embolism (3.6%). The cumulative rate of non-fatal thrombosis was 10.3%, without a difference between arterial and venous thrombosis [12] . Of note, in the most recent studies dealing with contemporary patients with PV [13] , post-diagnosis total major thrombosis rate was 2.62 per 100 patient-years, a figure lower than that reported in the ECLAP trial but comparable to the one in the more recent randomized clinical trial Cyto-PV where the rate was 2.7 per 100 patient-years [14] . In prospective studies in ET, fatal and non-fatal thrombotic events ranged from 0.9 to 2.6 per 100 patient-years. The incidence of arterial events was 2 to 3 times higher than that of venous events [15] [16] [17] .

In PMF, the prevalence of major thrombosis was assessed in 707 patients, followed in 4 European institutions. The overall cumulative rate of cardiovascular death and non-fatal thrombotic complications, corrected by competing for risk for mortality, was 2.23 events per 100 persons per year. No significant difference between non-fatal venous and arterial thrombosis was registered (0.76% and 0.86% patients per year, respectively) [18] .

A recent population-based study carried out in Sweden recruiting 9429 patients with MPNs and 35,820 matched control from 1987 to 2009, with follow-up to 2010, found that the HRs for arterial thrombosis among patients with MPNs compared with controls at 3 months, 1 year, and 5 years were 3.0 (95% CI 2.7-3.4), 2.0 (95% CI 1.8-2.2), and 1.5 (95% CI 1.4-1.6), respectively. The corresponding HRs for venous thrombosis were 9.7 (95% CI 7.8-12.0), 4.7 (95% CI 4.0-5.4), and 3.2 (95% CI 2.9-3.6). The rate was significantly elevated across all age groups and was similar among MPN subtypes [19] .

Since the risk of thrombosis in ET and PV varies substantially between patients, a personalized treatment based on a risk-stratified approach is recommended by NCCN and ELN guidelines [20, 21] .

In PV, two risk categories are considered: high (age > 60 years or thrombosis history present) and low (absence of both risk factors); in ET, the IPSET thrombosis scoring system, four risk categories identify patients at different thrombotic risks according to age, prior thrombosis, cardiovascular risk factors, and the presence of JAK2V617F mutation [22, 23] . Since the median survival in PMF is ∼6 years, ranging from < 2 to > 10 years, the relevant endpoint for current prognostic scoring systems in PMF is represented by survival. However, even though a scoring system of thrombosis is not available in PMF, risk factors were identified and included the presence of JAK2V617F mutation, leukocytosis, and prior vascular events [18] .

The implications of these findings during the pandemic in MPN patients without documented COVID-19 infection or symptoms have been considered in the ASH recommendations. The panel of experts suggests strict adherence to current treatment goals aimed at decreasing the risk of thrombosis (HCT < 45% in PV, antiplatelet therapy in all PV and ET patients if indicated according to the IPSET score, cytoreduction to achieve European LeukemiaNet levels of control (HCT < 45%, leukocytes < 10 × 10 9 /L, platelets < 400 × 10 9 /L)).

To our knowledge, the only study published so far on the incidence and risk factors of thrombosis and bleeding in MPN was conducted by the European LeukemiaNet and involved 162 patients, with ET (n = 48), PV (n = 42), PMF (n = 56), and pre-PMF (n = 16). Twenty-two episodes of major thrombosis (n = 15) and major bleedings (n = 7) occurring during the acute phase of the first wave of coronavirus infection were registered in this study [24••] .

Thromboses were reported in patients managed at home, during hospitalization in regular wards, or in intensive care units (ICU). Overall, only three arterial thromboses were documented whereas the majority were VTE (n = 12) detected with a significantly higher frequency in ET patients (n = 8/48, 16.7%) than in PV (n = 2/42, 4.8%) or MF (n = 2/56, 3.6%) (p = 0.031). All but two patients with VTE were receiving anticoagulation with low molecular weight heparin (LMWH) at low/intermediate (50%) or therapeutic doses (n = 50%).

Adjusting the competing risk of dying, the probability of total thrombosis progressively increased in the first month after COVID-19 diagnosis and reached 8.5% after 60 days of follow-up (Fig. 1) . The cumulative incidence rate at 10, 20, and 30 days was 4.1%, 5.9%, and 7.1%, respectively. In patients followed at home or in the regular ward, the rate was 1.0% and 2.8%, respectively, whereas it reached 18. Overall, these findings in MPN patients are hardly comparable with those reported in the general population, where the incidence rate largely depends on whether patients are specifically screened for the presence of thrombosis or only considers symptomatic thrombosis. This variability is exemplified either in observational studies or in systematic reviews and meta-analyses. One of the first investigations reported that patients followed in ICU and screened for thrombosis presented VTE in 39 of 75 (47%) [25••], a figure two-fold higher than in the MPN. This high rate was confirmed in a recent meta-analysis. A subgroup of 2500 patients utilizing systematic screening revealed a VTE prevalence of almost 50% of critically ill patients who received prophylactic or therapeutic LMWH doses [26] .

Conversely, in a multicenter retrospective study, 400 hospital-admitted COVID-19 patients (144 critically ill) managed in the real-world clinical practice and treated with prophylactic LMWH doses had symptomatic VTE in 7.6% and 4.8% in ICU or regular wards, respectively [27••] . This frequency was also confirmed in one of the latest meta-analysis. The incidence of VTE in critically ill patients was 8% for clinically relevant pulmonary embolism and 14% for proximal deep vein thrombosis [28] . These figures are substantially lower than those determined in other previous meta-analyses [29] and underscore the difficulty of making reliable comparisons with the estimates found in MPN.

On the other hand, the classic venous and arterial thromboses represent only part of the vascular problem in the context of COVID-19, in which multiple micro-thromboses in the heart, kidneys, and lungs are frequently found in autopsies series of patients with sudden death associated with rapid respiratory deterioration and multiorgan failure (MOF)

In the ELN study, the two most frequent fatal events were pneumonia and MOF, particularly in ET. The thrombosis rate was significantly higher than in PV/PMF, leading to a shorter survival probability (53% vs. 75% respectively (p = 0.05). Thus, the possibility that these events might be associated with organ micro-thrombosis cannot be excluded. In support of this hypothesis is the finding that the number of platelets in patients with ET was found remarkably lower at the time of the diagnosis of COVID-19 compared to the last follow-up of these patients, which had occurred about 40 days earlier. The platelet decline was associated with a higher mortality rate (p = 0.0010), mainly due to pneumonia (p = 0.005), reported in a higher proportion of ET than in PV and MF.

It should be noted that in ET patients, d-dimer values were significantly higher than in the ones without thrombosis (p < 0.0001) [24••] . These findings highlight the prevalent role of platelets in the thrombogenesis of MPN patients with COVID-19. The systemic endothelial vascular cell damage associated with the viral infection involving the complement activation, interaction of platelets, leukocytes, neutrophil extracellular trap (NET) formation vascular endothelium, and activation of blood coagulation may be the basis for classic arterial and venous thrombosis, but also to lung and other tissues vessel occlusions and hypoxia [32] .

MPN patients are also at a higher risk for major bleeding, which can occur as the heralding presentation in 6.2% of cases, with a pooled prevalence of 8.9% in PMF, 7.3% in ET, and 6.9% in PV [11] . During the follow-up, up to 4% of MPN patients have a hemorrhagic complication within the first decade after diagnosis [33] .

During COVID-19, the ELN study reported 7 of 162 (4.3%) patients who developed major bleeding, particularly in MF, where all the hemorrhagic episodes required blood transfusions (Fig. 1) . These estimates are slightly higher than that reported (2.3%) in the general population with COVID-19 [27••] . Interestingly, while the peak of thrombotic events occurred in the first days after COVID-19 diagnosis, bleeding episodes were reported later, starting 2 weeks afterward. Of note, in 4 of the 7 cases, coagulopathy or severe thrombocytopenia was associated with severe bleeding, suggesting caution on the empiric intensification of LMWH, particularly in PMF [24••] .

To prevent VTE, guidelines from international scientific organizations recommended anticoagulant thromboprophylaxis with LMWH in all hospitalized COVID-19 patients in the absence of contraindications [34, 35•, 36•, 37•, 38] . Nevertheless, since the beginning of the pandemic, clinicians had the perception that standard dosages of this drug were not adequate to prevent VTE in patients with COVID-19 and higher LMWH doses were proposed [25 ••, 39, 40] .

Advantages of LMWH prophylaxis were quantitatively documented in a meta-analysis on 25,719 hospitalized COVID-19 patients, in whom a 50% reduction of Fig. 1 Cumulative incidence of thrombosis and bleeding during the acute phase of COVID-19, overall and by MPN phenotypes in-hospital mortality was shown (pooled risk ratio, RR 0.50, 95% CI 0.40-0.62). Interestingly, in ICU patients, a substantial reduction of the thrombotic rate was ascertained when LMWH was administered at therapeutic dosage compared to the prophylactic one. In-hospital mortality decline was demonstrated (RR: 0.30, 95% CI: 0.15-0.60; I 2: 58%). However, new evidence from the REMAP-CAP randomized controlled trial indicates that for patients with severe disease who required ICU support, therapeutic anticoagulation did not improve outcomes and increased bleeding risk [41•] .

Given the safety concern, the empiric use of full-dose LMWH is discouraged outside of a clinical trial, even in critically ill MPN patients with COVID-19, such as the subgroup with ET who would typically be admitted to an ICU. The approach of administering intermediate regimens of LMWH in high-risk MPN with COVID-19 patients may be considered in particular high-risk patients with high-risk features and rapid respiratory deterioration while on prophylactic LMWH dose. This was not confirmed in the REMAP-CAP randomized controlled trial in which ICU patients with severe COVID-19 receiving therapeutic anticoagulation did not improve and substantially increased the bleeding risk.

In the INSPIRATION study that enrolled 600 patients admitted to the ICU with COVID-19, prophylaxis with intermediate-dose anticoagulation, compared with the standard-dose, failed to show a significant difference in a composite endpoint of venous or arterial thrombosis, treatment with extracorporeal membrane oxygenation, or mortality within 30 days [42 ••] . Thus, based on this latter result, the routine empirical use of intermediate-dose prophylactic anticoagulation in unselected patients admitted to the ICU with COVID-19 is not recommended in the general population.

It is complicated to compare these data obtained in the general population with SARS-CoV-2 infection of patients with MPN. The only study that details the efficacy risk profile of heparin prophylaxis in MPN with COVID-19 concerns only 162 patients, of which 19 experienced 22 thrombo-hemorrhagic events at home (n = 1/40, 2.5%), or during hospitalization in regular wards (n = 13/105, 12.4%) or in ICU (n = 5/17, 29.4%) (p = 0.016). All but two patients with VTE were receiving LMWH anticoagulation with low/intermediate (n = 5/10, 50%) or therapeutic doses (n = 5/10, 50%). Mortality was recorded in 7 of 14 (50%) patients with thrombosis, of which one was attributed to acute myocardial infarction. At the same time, the other six were not considered to be related to the vascular events [24••] .

Therefore, we believe that, in line with the recommendations contained in the latest ASH version of the international panel of MPN experts [43], all hospitalized patients should receive prophylactic doses of LMWH.

In Fig. 2 , some scenarios are presented that affect patients with MPN and COVID-19 stratified according to their history of thrombosis and treated at home or in hospital. In the case of non-severe COVID-19, antithrombotic regimen prophylaxis is that recommended independently on COVID-19. In contrast, in patients with medium/high severity of the infection, prevention can be diversified, and, in some cases, MPN patients with COVID-19 may need higher doses of heparin.

High-risk condition concerns hospitalized patients with MPN treated with both anti-vitamin-K antagonists (VKA) or direct oral anticoagulants (DOACs) for pre-COVID-19 VTE chronic atrial fibrillation. In these cases, depending on the severity of the COVID-19 and patient circumstances, oral anticoagulants could be replaced with intermediate/therapeutic doses of heparin. If the patient's condition with MPN-COVID allows it, the question may arise to continue VKA or DOACs even during the acute phase of the infection. However, given the possible interaction of these drugs with most antiretroviral drugs on liver cytochromes, such as CYP2C9 and CYP3A4, the advice is to replace oral anticoagulants with LMWH prophylaxis.

Intermediate/high doses of heparin can also be considered in ICU patients with ET associated with a rapid decline of the number of platelets and progressive respiratory failure, mainly if the clinical worsening had arisen despite the use of heparin at prophylactic doses. Notably, the higher incidence of VTE observed in ET than PV and PMF was associated with higher mortality risk and may justify some risks of bleeding.

In addition to its antithrombotic efficacy, aspirin was shown to reduce the risk of ARDS mortality [44] . This impact might not be due to the antithrombotic action but rather to its antiviral and anti-inflammatory properties, which are not possessed by other antiplatelet drugs [45• ]. Moreover, a study showed that aspirin users exhibited a quicker recovery of the acute phase of the infection in comparison with non-users (p = 0.045), a less frequent need of mechanical ventilation (adjusted HR 0.56, 95% CI 0.37-0.85, p = 0.007), ICU admission (adjusted HR 0.57, 95% CI 0.38-0.85, p = 0.005), and in-hospital mortality (adjusted HR 0.53, 95% CI 0.31-0.90, p = 0.02). Most importantly, no excess of major bleeding was documented (p = 0.69) [46••] .

These findings will have to be exploited in the prophylaxis of thrombosis in patients with MPN, particularly in ET, given its higher incidence of venous events during COVID-19. Unfortunately, it will be difficult to conduct appropriate comparative intervention studies in this setting due to the rarity of these diseases. However, the use of aspirin could be proposed, even in the association with LMWH unless contraindications, occurring during in-hospital stay (acute liver failure, severe renal failure, severe thrombocytopenia, documented drug interactions, planned invasive procedures).

In patients with pre-COVID-19 arterial thrombosis (transient ischemic attack, ischemic stroke, myocardial infarction, peripheral arterial thrombosis) or with percutaneous coronary intervention (PCI) (within ≤ 3 months), it is strongly recommended not to discontinue antiplatelet drugs, unless clinical circumstances or hemorrhagic events prevent it [47] .

Since thrombocytopenia is a frequent finding during COVID-19 and particularly in PMF and in 23% of patients is severe (under 50 × 10 9 /L) [24••] , strict monitoring of the platelet count is warranted, particularly in MPN patients in need of cytoreductive therapy.

Venous thromboembolism and arterial thrombosis constitute a significant concern in some categories of patients with SARS-CoV-2 infection. Although the available data is scarce, an association between ET and thrombosis, regardless of inflammation indicators and the severity of COVID-19, was shown. Moreover, bleeding is mainly reported in MF. The limited incidence of thrombotic events did not allow to produce robust evidence on the use of heparin or antiplatelet drug prophylaxis. Therefore, we must draw some considerations and suggestions based on the evidence produced by the numerous studies conducted in the general population. Our advice is in line with ASH recommendations and indicates that MPN patients with COVID-19 treated at home should receive the recommended routine prophylaxis. VKAs or DOACs prescribed for pre-COVID venous thrombosis or atrial fibrillation should be replaced with LMWH at intermediate/therapeutic doses in the case of hospitalization.

Given that MPNs are more prone than other patients to developing heparin-induced thrombocytopenia [48, 49] , strict platelet count surveillance is warranted.

Conflict of Interest Tiziano Barbui and Valerio De Stefano declare that they have no conflict of interest. Human and Animal Rights and Informed Consent This article does not contain any studies with human or animal subjects performed by any of the authors.

Prevalence of venous thromboembolism in patients with severe novel coronavirus pneumonia

Pulmonary post-mortem findings in a series of COVID-19 cases from northern Italy: a two-centre descriptive study

Predictors of in-hospital COVID-19 mortality: a comprehensive systematic review and meta-analysis exploring differences by age, sex and health conditions

High mortality rate in COVID-19 patients with myeloproliferative neoplasms after abrupt withdrawal of ruxolitinib

Risk of infections in patients with myeloproliferative neoplasms-a population-based cohort study of 8363 patients

Outcomes of patients with hematologic malignancies and COVID-19: a systematic review and meta-analysis of 3377 patients

Case-fatality rate and characteristics of patients dying in relation to COVID-19 in Italy

Comorbidities and mortality in patients with COVID-19 aged 60 years and older in a university hospital in Spain

Mortality among US patients hospitalized with SARS-CoV-2 infection in 2020

Clinical characteristics and risk factors associated with COVID-19 severity in patients with haematological malignancies in Italy: a retrospective, multicentre, cohort study

A systematic review and metaanalysis of the prevalence of thrombosis and bleeding at diagnosis of Philadelphia-negative myeloproliferative neoplasms

Vascular and neoplastic risk in a large cohort of patients with polycythemia vera

In contemporary patients with polycythemia vera, rates of thrombosis and risk factors delineate a new clinical epidemiology

Cardiovascular events and intensity of treatment in polycythemia vera

United Kingdom Medical Research Council Primary Thrombocythemia 1 Study. Hydroxyurea compared with anagrelide in high-risk essential thrombocythemia

Anagrelide compared with hydroxyurea in WHO-classified essential thrombocythemia: the ANAHYDRET Study, a randomized controlled trial

United Kingdom Medical Research Council Primary Thrombocythemia-1 Study; United Kingdom National Cancer Research Institute Myeloproliferative Neoplasms Subgroup; French Intergroup of Myeloproliferative Neoplasms; the Australasian Leukaemia and Lymphoma Group

Thrombosis in primary myelofibrosis: incidence and risk factors

Risk for arterial and venous thrombosis in patients with myeloproliferative neoplasms: a population-based cohort study

Myeloproliferative neoplasms, version 2.2017, NCCN Clinical Practice Guidelines in Oncology

Philadelphia chromosome-negative classical myeloproliferative neoplasms: revised management recommendations from

Development and validation of an International Prognostic Score of thrombosis in World Health Organization-essential thrombocythemia (IPSET-thrombosis)

Practice-relevant revision of IPSET-thrombosis based on 1019 patients with WHO-defined essential thrombocythemia

Among classic myeloproliferative neoplasms, essential thrombocythemia is associated with the greatest risk of venous thromboembolism during COVID-19

Incidence of venous thromboembolism in hospitalized patients with COVID-19

Prevalence of venous thromboembolism in critically ill COVID-19 patients: systematic review and meta-analysis

COVID-19 and coagulation: bleeding and thrombotic manifestations of SARS-CoV-2 infection

Risk of clinically relevant venous thromboembolism in critically ill patients with COVID-19: a systematic review and meta-analysis. Front Med (Lausanne)

A meta-analysis of the incidence of venous thromboembolic events and impact of anticoagulation on mortality in patients with COVID-19

Multiorgan and renal tropism of SARS-CoV-2

Endothelial cell infection and endotheliitis in COVID-19

Platelets: bridging thrombosis and inflammation

Risk factors for and management of MPN-associated bleeding and thrombosis

Thromboprophylaxis: balancing evidence and experience during the COVID-19 pandemic

Scientific and Standardization Committee communication: clinical guidance on the diagnosis, prevention, and treatment of venous thromboembolism in hospitalized patients with COVID-19

Prevention, diagnosis, and treatment of VTE in patients with coronavirus disease

Clinical guidance for prevention and treatment of venous thromboembolism in COVID-19 patients from the ASH

Prophylaxis and treatment of COVID-19 related venous thromboembolism

Incidence of thrombotic complications in critically ill ICU patients with COVID-19

Increasing dosages of low-molecular-weight heparin in hospitalized patients with Covid-19

Different anticoagulant regimens, mortality, and bleeding in hospitalized patients with COVID-19: a systematic review and an updated meta-analysis

Effect of intermediate-dose vs standard-dose prophylactic anticoagulation on thrombotic events, extracorporeal membrane oxygenation treatment

Aspirin therapy in patients with acute respiratory distress syndrome (ARDS) is associated with reduced intensive care unit mortality: a prospective analysis

Use of antiplatelet drugs and the risk of mortality in patients with COVID-19: a meta-analysis

Aspirin use is associated with decreased mechanical ventilation, intensive care unit admission, and in-hospital mortality in hospitalized patients with coronavirus disease 2019

Italian Study Group on Atherosclerosis, Thrombosis, Vascular Biology. Questions and Answers on Practical Thrombotic Issues in SARS-CoV-2 Infection: A Guidance Document from the Italian Working Group on Atherosclerosis, Thrombosis and Vascular Biology

Anti-platelet factor 4/heparin antibody formation occurs endogenously and at unexpected high frequency in polycythemia vera

High prevalence of heparin induced thrombocytopenia with thrombosis among patients with essential thrombocytemia carrying V617F mutation

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