key: cord-0799681-1dvco7wp
authors: Al Bitar, Samar; Ballouz, Tala; Doughan, Samer; Gali-Muhtasib, Hala; Rizk, Nesrine
title: Potential role of micro ribonucleic acids in screening for anal cancer in human papilloma virus and human immunodeficiency virus related malignancies
date: 2021-07-22
journal: World J Gastrointest Pathophysiol
DOI: 10.4291/wjgp.v12.i4.59
sha: 2855a23b8530feaf81602a50b907e774195d6b42
doc_id: 799681
cord_uid: 1dvco7wp

Despite advances in antiretroviral treatment (ART), human immunodeficiency virus (HIV) continues to be a major global public health issue owing to the increased mortality rates related to the prevalent oncogenic viruses among people living with HIV (PLWH). Human papillomavirus (HPV) is the most common sexually transmitted viral disease in both men and women worldwide. High-risk or oncogenic HPV types are associated with the development of HPV-related malignancies, including cervical, penile, and anal cancer, in addition to oral cancers. The incidence of anal squamous cell cancers is increasing among PLWH, necessitating the need for reliable screening methods in this population at risk. In fact, the currently used screening methods, including the Pap smear, are invasive and are neither sensitive nor specific. Investigators are interested in circulatory and tissue micro ribonucleic acids (miRNAs), as these small non-coding RNAs are ideal biomarkers for early detection and prognosis of cancer. Multiple miRNAs are deregulated during HIV and HPV infection and their deregulation contributes to the pathogenesis of disease. Here, we will review the molecular basis of HIV and HPV co-infections and focus on the pathogenesis and epidemiology of anal cancer in PLWH. The limitations of screening for anal cancer and the need for a reliable screening program that involves specific miRNAs with diagnostic and therapeutic values is also discussed.

At the end of 2019, approximately 36900000 people were estimated to be living with human immunodeficiency virus (HIV) [1] . Despite the advances in antiretroviral treatment (ART) and the increase in number of patients accessing ART since 2010, cancer mortality in people living with HIV (PLWH) remains high [2] . Mortality from HIV associated illnesses decreased substantially since its peak in 2004 mainly due to a decrease in the incidence of opportunistic infections. With the introduction of highly active ART in 1996, there has been a substantial improvement of clinical outcomes in PLWH [3] [4] [5] . This has brought an increase in life expectancy and a change in the age distribution of PLWH [6, 7] . The risk of developing cancer increases with age; and as PLWH are now aging, the burden of cancer has substantially increased in this population. Since the beginning of the epidemic, HIV was associated with Kaposi's sarcoma, aggressive B-cell lymphomas, and invasive cervical cancer. Diagnosis of these cancers in PLWH confers the diagnosis of acquired immune deficiency syndrome (AIDS) and are thus termed as AIDS-defining cancers. Other types of cancers are non-AIDS defining, such as anal carcinoma, Hodgkin lymphoma, hepatocellular carcinoma, and lung cancer. These have been increasingly recognized to occur in PLWH and have become a leading cause of death [8] [9] [10] [11] . One reason behind the increase in the rate of non-AIDS defining cancers in PLWH is increased prevalence of oncogenic viruses in this population, one of which is human papilloma virus (HPV) [12] .

HPV is the most common sexually transmitted viral disease in both men and women worldwide [13] . HPV targets epithelial cells and includes more than 200 types that exist with genomic differences. About 40 types specifically infect the anogenital epithelium and upper digestive tract, among which 15-20 types are considered as high-risk HPV (HR-HPV), including HPV16 and HPV18 [14] . Oncogenic or HR-HPV types are associated with the development of high-grade intraepithelial lesions and consequently, cancers of the anogenital region and oropharynx. About 99.9% of cervical cancers and 80%-90% of anal squamous cell cancers (ASCC) are associated with infection with HR-HPV [15] . While the incidence of cervical cancer has remained stable over the years, the incidence of ASCC has increased, particularly in PLWH [16] . With these increasing trends, it is imperative to screen for anal cancer in this high-risk population. However, many of the currently used screening methods, including the Pap smear, are invasive and require specialized equipment. In addition, the Pap smear is neither specific (specificity is approximately 75%) nor sensitive (approximately 55%) [17] . Thus, identification of non-invasive and more effective methods is crucial.

Micro ribonucleic acids (miRNAs) have emerged as clinically useful molecular biomarkers for better management and treatment of many types of cancers. In HPVassociated cancers, miRNAs have been shown to be deregulated and involved in the pathogenesis of the disease. Given that the molecular mechanisms involved in anal cancer development during HIV infection are still unclear, characterization of miRNA expression in the context of HIV infection and anal cancer and the identification of relevant biomarkers could help elucidate the potential role of HIV and HPV in the progression of ASCC, as well as help prevent and treat anal cancer.

In this review, we will focus on the mechanisms and pathogenesis underlying HIV and HPV infections and the epidemiology and risk factors of anal cancer. We will also discuss the need for anal cancer screening, especially in HIV-infected individuals and the potential implementation of miRNAs as screening and therapeutic tools in highrisk populations.

HIV-1 is the causative agent of AIDS. HIV-1 is a retrovirus whose genome is composed of 2 copies of single-stranded RNA molecules. HIV genome has 9 open reading frames and encodes for precursor proteins that give rise to 15 viral proteins. These proteins can be classified into structural and regulatory. The structural proteins include Gag, Env, and Pol. The matrix, capsid (CA), nucleocapsid, and p6 proteins are generated from Gag precursor and make up the core of the virus particle. The Env polyprotein is subsequently processed to generate the envelope proteins, gp120 and gp41. The pol gene encodes viral enzymes: Protease (PR), reverse transcriptase, and integrase. The HIV genome also encodes essential regulatory elements, Tat and Rev, and accessory regulatory proteins: Vif, Vpr and Nef [18] .

HIV envelope glycoprotein mediates HIV cell entry by binding to its primary receptor, CD4 molecule, expressed on target cells, such as CD4+ T cells, monocytes, and macrophages. HIV entry also requires binding a chemokine coreceptor, CCR5 or CXCR4 [19] . Viral entry is followed by reverse transcription of the viral RNA genome, integration of the provirus into cellular genome, synthesis of viral genome, and assembly and budding of the newly formed virions. When no new viral proteins are produced, infected cells can revert to latency [20] .

HIV targets and kills CD4+ T cells, monocytes, macrophages, and microglial cells, however the main targets of HIV infection and subsequent destruction are the CD4+ T cells [21, 22] .

The mechanisms underlying CD4+ T cell death are still not well defined. The permissivity status of CD4+ T cells during HIV infection determines the pathway by which these cells die (Figure 1 ). Abortively-infected[23,24], productively infected[25-28], and HIV-uninfected (bystander) CD4+ T cells undergo cell death through different mechanisms [27] .

In addition to progressive CD4 lymphopenia, HIV infection is also associated with impaired HIV-specific CD8+ T cell responses. CD8+ T cells play an important role in eliminating viruses. Recognition of infected cells occurs through T cell receptor that binds processed viral antigen expressed by major histocompatibility complex (MHC) I molecules on the surface of infected cells. Recognition is followed by a cascade of activation events leading to the release of granzymes and perforin and killing of infected cell. Activated CD8+ T cells also release anti-viral cytokines that act to control viral replication [29] . Despite the over activation of the immune system during HIV infection, it seems that HIV-specific CD8+ T cell responses fail to clear viral infection [30] [31] [32] and this can be attributed to several factors. HIV-infected cells sometimes revert to latency and are known to act as viral reservoirs. In this case, the absence of HIV protein expression on the surface of infected cells hinders recognition by CD8+ T cells [33] . Interestingly, several studies have shown that HIV proteins are capable of escaping CD8+ T cell recognition by modulating the expression of MHC I on surface of infected cells (Figure 2) procaspase-8 and generate Casp8p41. This fragment activates the transcription factor NF-kappaB inhibitor and thus, induces HIV replication by upregulating HIV long terminal repeats (LTRs). Casp8p41 expression promotes inflammatory responses by enhancing the production of pro-inflammatory cytokines. Besides, Casp8p41 induces apoptosis by directly co-localizing with the mitochondria and depolarizing its outer membrane. The subsequent release of cytochrome c from mitochondria leads to activation of caspase 9 and cell death. Another mechanism by which viral integration triggers cell death is through the activation of deoxyribonucleic acid-dependent protein kinase, resulting in phosphorylation of p53 and activation of p53-dependent cell death program. HIV Tat, Nef, and Vpr can have both pro-and anti-apoptotic effects. On the other hand, gp120, Vpu, and protease have pro-apoptotic effects. HIV-infected cells can be killed by cytotoxic lymphocytes, natural killer cells and CD8+ T cells, which become highly active during infection; C: HIV-uninfected cells, known as bystander cells, usually die by apoptosis during the course of infection, due to either: upregulation of death ligands (Fas, TRAIL, TNF), activation-induced cell death due to chronic inflammation and over activation of immune cells, or direct cytotoxic effects of soluble HIV proteins (Tat, gp120). DNA-PK: DNA-dependent protein kinase.

CD8+ T cell function. Several inhibitory molecules ( Figure 2) 

HPV can deregulate cellular proteins, including p53 and Retinoblastoma protein (pRb), thus mediating epithelial transformation and malignancy. HPV genome consists of a circular DNA that encodes the early proteins E1, E2, E4, E5, E6, and E7, and the late proteins L1 and L2 ( Figure 3 ). E1 and E2 play an important role during HPV replication by binding to the viral replication origin, whereas E4 proteins are involved in virion release. E5, E6, and E7 are viral oncoproteins whose increased expression and activity is associated with enhanced proliferation of HPV-infected epithelial cells. L1 and L2 are structural proteins that form the viral capsid[47]. in HPV-infected keratinocytes. HPV E5 oncoprotein downregulates major histocompatibility complex (MHC) II expression and sequesters human leukocyte antigen (HLA) class I complexes in keratinocytes, thereby blocking transport of HLA class I complexes to cell membrane surface. HPV E6 exerts oncogenic effects, mainly through deregulation of p53 and induction of cell cycle arrest. HPV E7 exerts oncogenic activity through deregulating pRB. It promotes downregulation of MHC class I expression through downregulating peptide transporter 1 associated with antigen processing (TAP1) and binding to TAP transporter, and thus inhibiting peptide loading into MHC I antigens. It also downregulates promotor of MHC I heavy chain. Importantly, HIV Tat 

ASCC are cancers that arise in the transitional or squamous zone of the anal canal and are mostly caused by HPV16 and 18. It is believed that the basal layer cells in the epithelium of this transitional zone can become infected with HPV after the occurrence of micro-abrasions. Most individuals who acquire HPV mount the appropriate immune response and clear HPV infection within a year. However, HPV may persist in others and could lead to either low-grade or high-grad (HSIL) squamous intraepithelial lesions , and can be further classified into anal intraepithelial neoplasia (AIN) 1, 2 or 3[69,70].

Anal cancer is uncommon with 48541 new cases reported worldwide in 2018 as by the GLOBOCAN estimates[71]. However, its epidemiology has changed over the past 2 decades. A steady increase in the incidence and prevalence rates of ASCC has been reported. In the United Kingdom, a 70% increase in its incidence rates has been noted since the early 1990s [72] . The United States has reported similar trends with a 2.9% increase in incident rates each year since 1975 [73] . In 2021, there will be an estimated 9090 new anal cancer cases and 1430 new anal cancer deaths [74] . The increase in incidence has been associated with multiple factors that include lifetime number of sexual partners, smoking, receptive anal intercourse, genital warts, and infection with HIV[75-77]. More than 90% of ASCCs have been found to be related to HPV, mainly HPV 16 and 18. Among men, the highest proportion of HPV is in men who have sex with men (MSM) and ranges between 50%-60%[76,78]. This proportion is even higher in HIV-infected MSM and reaches 90% in some studies[79,80]. Additionally, this population is infected with multiple HR-HPV types [81, 82] . Not surprisingly, the prevalence of HSIL and anal cancer mirrors that of anal HPV in these populations where the incidence of neoplasia is higher than that of the general population. Compared to heterosexual men, MSMs have a 20 times increased risk of developing ASCC. HIV-positive MSMs have an even greater risk [76] .

An obvious relationship between HIV, HPV, and anal cancer was illustrated in a population study in the United States between 1980-2005[73] . Authors found that HIV infection had a strong impact on the trends of anal cancer among males where incidence rate increased by 3.4% annually overall and by 1.7% in those without HIVinfection. A meta-analysis of 53 studies by Machalek et al [76] assessed the prevalence and incidence of HPV, AIN, and anal cancer in MSM and reported a substantial difference between HIV-positive and HIV-negative men for prevalence of any type of HPV (P = 0.005), including any HR-HPV (P = 0.01), prevalence of any anal cytological abnormality (P = 0.005), and low-grade anal lesions (P = 0.01). Analysis of recent studies reporting on histological abnormalities, high-grade AIN, and anal cancer revealed a significant difference between HIV-positive MSM vs HIV-negative MSM [76] . In another study, the incident rate for anal cancer was reported to be 69 per 100,000 person-years (PY) in HIV-positive MSMs vs 14 per 100,000 PY in HIV-negative MSMs [83] . Contrary to AIDS defining cancers, whose rates have decreased after the introduction of ART, the incidence rates of ASCC have shown an increase by 3% [8, 76, 84] . This may be attributable to a longer lifespan of PLWH allowing them to live longer with oncogenic HPV giving time for the development of HSIL and ASCC. In addition, PLWH have been found to have multiple types of HPV with Müller et al [82] reporting PLWH having a 7 times higher risk of having multiple types of HPV as compared to HIV-negative individuals.

The link between a lower rate of HR-HPV clearance and development of ASCC in PLWH is still being investigated. Studies have shown that in HIV-positive individuals, HR-HPV infection is cleared at a slower rate than HIV-negative individuals. Geskus et al [85] observed that HPV16 had the lowest clearance for both prevalent positive and incident positive infection. Additionally, authors reported a decreasing clearance rate with increasing HIV viral load. Results from a recently updated meta-analysis showed that clearance rate of HPV infection among PLWH was approximately half compared to that of HIV-negative individuals, with similar findings reported for HR-HPV[86]. Whether CD4 count affects the clearance rate or not is not well established. In the same meta-analysis above, Looker et al [86] reported a possible, but non-significant, reduction in clearance of HPV with lower CD4 counts. In a nested case-control study from the Swiss HIV Cohort Study, lower CD4 counts in PLWH were correlated with the development of ASCC. Authors reported that the best predictor was a CD4 count 6-7 years prior to ASCC diagnosis. Beyond that point, authors found that the ASCC risk was less sensitive to CD4 counts, highlighting the importance of starting ART early before the establishment of precancerous lesions[87].

There are no formal guidelines on anal cancer screening due to the lack of trials assessing the effectiveness of such screening practices. However, with the accumulating evidence of an increasing incidence of anal cancer in PLWH, there is increased advocacy for screening in these high-risk populations, drawing on the proven value of cervical cytology in reducing cervical cancer. Additionally, cost-effective models of screening MSM for AIN every 2-3 years have shown possible gains in life-expectancy and quality of life [88, 89] .

Screening consists of detection and treatment of anal HSIL. Detection can be done through anal cytology, digital rectal examination, high resolution anoscopy (HRA), and/or biopsy. While some experts have advocated the use of HRA for initial screening because of the high prevalence of AIN in PLWH and MSM, anal cytology remains a preferred initial method due to limited availability of HRA especially in developing countries [64] . Yet, all the previously mentioned tools have several limitations and disadvantages. For example, HRA is invasive, and cytology is neither sensitive nor specific. Therefore, a non-invasive method with high sensitivity and specificity for detection of precancerous and cancerous anal lesions is needed. July 22, 2021

Volume 12 Issue 4

Circulatory and tissue miRNAs have become of interest to investigators, as these small non-coding RNAs possess distinctive properties that make them ideal biomarkers for detection and prognosis of cancer. [104] cancers, which have allowed for the identification of a series of miRNAs that are deregulated in these cancers. However, an invasive method, such as surgery and biopsy collection, is needed to analyze the tissues. Thus, researchers are investigating the use of plasma and serum miRNAs as potential circulatory biomarkers for different purposes. This would allow for non-invasive quantification of these biomarkers and potentially for detection of premalignant lesions and screening of early tumorigenesis. In the context of HPV-associated cancer, miRNAs have been studied and documented as mediators or suppressors of pathogenesis[96,97,105]. Some of these miRNAs have been shown to be deregulated by HPV E5, E6, and E7 oncoproteins in different cells and tissues (Table 1) . By downregulating p53, E6 alters the expression of many miRNAs that are transcribed by p53. On the other hand, E7 releases E2F transcription factor from pRB-E2F complex by degrading pRB. As a result, E2F becomes free to activate the transcription of many miRNAs. The mechanism by which E5 deregulates cellular miRNAs is still unclear [106] . The deregulation of many of these miRNAs was shown to affect several hallmarks of cancer, including enhanced proliferation, inhibition of apoptosis, invasion, and metastasis. A recent study showed that miR-129 was significantly upregulated in the serum and cervical cancer tissues collected from 72 patients, suggesting the possibility of using this miRNA as a biomarker for the detection of cervical cancer. Interestingly, HPV typing detected HPV16 in all cancer samples studied [107] . Another study identified a miRNA signature panel consisting of 9 miRNAs (miR-9, miR-15b, miR-20a, miR-31, miR-93, miR-183, miR-184, miR-222, and let-7b) with a combined area under the curve of 0.89 for CIN3 detection in HPVpositive self-samples of women with CIN3[108]. Recently, Shi et al [109] identified an optimal subset of 7 signature miRNAs, including miR144, miR147b, miR2182, miR425, miR451, miR483, and miR486 in cervical cancer. Functional enrichment analysis showed that the latter miRNAs are involved in carcinogenic pathways, such as Wnt signaling pathway and transforming growth factor-β signaling pathway. Importantly, altered miRNAs have been investigated mainly in cervical cancer cell lines[96] and cervical carcinoma samples [110] . However, miRNAs have been less studied in anal cancer and a single study showed that HPV16-E7 protein is capable of inducing miR-15b in anal carcinoma biopsies [111] .

HIV infection also dysregulates cellular miRNA biogenesis and expression profiles (Table 3) . On the other hand, cellular miRNAs also target HIV genome, but it is still unclear whether these miRNAs are effective during HIV infection [112] .

Overall, molecular mechanisms that contribute to anal cancer pathogenesis and progression are still elusive. One of the reasons that little progress has been made in understanding the mechanisms of carcinogenesis in this type of cancer is the scarcity of in vitro and in vivo model systems for investigating anal cancer. Thus, further studies are required to gain insight into the mechanisms involved in anal caner. This is particularly important as these mechanisms may involve miRNAs, which may be further investigated as potential targets for cancer therapy. The use of miRNA-based therapeutics has been investigated in clinical trials in several countries. MicroRNA mimics and anti-miRNAs (antagomirs) are now under investigation as potential therapeutic agents for multiple cancers. miRNA mimics may be administered to replace downregulated miRNAs, which usually act as tumor suppressors in cancers. On the other hand, many miRNAs have been targeted for inhibition in the treatment of several cancers. These miRNAs are referred to as oncomiRs and their overexpression in cancer contributes to pathogenesis. In the context of cervical cancer, Lee et al [110] showed that treatment with anti-miR-199a suppressed cervical cell growth in vitro. Additionally, a study has shown a promising role for the tumor suppressor miR-34a, which is downregulated in HPV-positive cancers, in repressing oncogenic transformations. Both miR-34a and miR-125 are downregulated in cervical cancer samples and correlate with cervical cancer invasiveness[120]. Interestingly, a recent phase 1 study of MRX34, a liposomal miR-34a mimic, was conducted with patients having advanced solid tumors [121] . Thereby, this miRNA may be a good candidate for treatment of HPV-related cancers, including anal cancer [122] . In addition, anti-miRs targeted at miR-122, which has been shown to be upregulated in HIV-1 infected Jurkat cells, reached clinical phase II trials and were investigated for treating hepatitis C infection [123] . Other candidate miRNAs are being tested in clinical trials, paving the way for developing miRNA-based drugs for treating several illnesses and cancer diseases [122, 124] .

In the case of anal cancer, where HIV and HPV pathogenesis play a role in the development of the disease in PLWH, a major challenge is to distinguish HIV-specific miRNAs, HPV-specific miRNAs, and HIV and HPV co-infection-specific miRNAs. Major limitations include the absence of studies implementing computational models to identify these miRNAs, technical issues associated with conventional miRNA extraction and detection tools, and scarcity of anal cancer in vitro and in vivo models. Ongoing studies are still being conducted to study miRNA profiles during HIV [125] [126] [127] and HPV [128] [129] [130] infections. With the appropriate application of advanced bioinformatic analysis tools and computational models, the identification of the most predictive miRNAs, even from complex datasets would be possible. These tools are becoming widespread and have already been used to identify potential miRNA biomarkers for Ebola[131] and severe acute respiratory syndrome coronavirus 2[132], in addition, these tools have been used to decipher potential miRNA biomarkers in a wide variety of cancers, including melanoma [133, 134] , breast [135] , colon [136] , and lung cancer [137] .

In addition to the conventional miRNA detection platforms which include Northern blotting, in situ hybridization, next generation sequencing, reverse transcription qPCR, and microarrays [138] , new miRNA extraction and detection platforms have emerged to compensate for the limitations of conventional assays [139] . These technologies are referred to as point-of-care (PoC) technologies and include isothermal amplificationbased assays [140] , lateral flow assay-based systems[141], nanobead-based [142] , electro-chemical-based [143] , and microfluidic chip-based [144] strategies. The latter, which is also known as Lab-on-a-chip or microchip, is highly specific, cost-effective, and a quick approach for the multiplexed detection of miRNAs [139] . It has been used to test miRNAs in several biological samples, including blood of breast cancer patients [145] . Importantly, this system has been also used to quantify miRNAs in plasma extracellular vesicles (EVs), including exosomes. EVs are secreted by body cells and are found in body fluids including plasma, urine, and synovial fluid [146] . They have been shown to carry and stabilize miRNAs in the blood [147] . A unique feature of exosomes is the presence of cell-specific proteins [148] , which enables identification of exosomes released from cancer cells. Examining specific miRNAs released from tumors and tumor niche, instead of whole blood miRNA profiling would provide a more accurate way of distinguishing HIV-specific and HPV-specific miRNAs, given the unique viral tropism of each. Exosomal miRNAs would enable the identification of the cell origin and might be a better source when compared to non-exosomal, cell-free miRNAs. Recently, studies that profiled and analyzed miRNAs from different sources were reviewed [149] . Authors concluded that 71% of the studies stated that exosomes are the best source of miRNAs as biomarkers. Detecting EVs miRNA signature has already been proven to be a good prognostic tool in several cancers including colorectal [150] and pancreatic cancer [151] .

Interestingly, organ-on-chip and organoids are being used to study infectious diseases and cancer. These models can be used to assess HPV virus-Langerhans cells interactions [152] and HPV-oral mucosa epithelia interactions [153] . Cell-to-cell communication can be also studied by co-culturing cancer cells with immune cells, and thus allows the study of cancer-immune interaction. Organoids can be used to model tumor-derived EVs, also known as oncosomes, in addition to EVs released by stromal cells in tumor microenvironment [154] . Very recently, researchers established organoid cultures from human ecto-and endocervix. Cells collected using Pap brush method were used to derive organoids from cervical tissue. The established patientderived model system resembled causative HPV infection [155] , and thus could be used for modeling HPV-related pathogenesis, in addition to exploring the role of HPV and HIV in deregulating miRNAs. The same derivation method can be used to derive organoids from healthy or tumor anal tissue to assess miRNA deregulation by HIV and/or HPV. These model systems could be used to test the efficacy of engineered miRNA-loaded EVs in targeting anal cancer cells to deliver potential miRNA therapeutic molecules .

It is important to note that although extensive research has been conducted to identify candidate miRNA biomarkers for cancer screening, the development of new techniques, such as PoC for miRNA detection is still at the very early stage and a work on progress. Further progress is required to achieve the desired goal of using PoC testing for detecting and distinguishing miRNAs deregulated by oncogenic viral infections, including HPV. Therefore, the identification of miRNAs deregulated by HIV, HPV, and HIV-HPV co-infection warrants further research. More accurate and standardized methods are required for implementation of miRNAs as biomarkers for anal cancer diagnosis [181] [182] [183] [184] [185] [186] [187] [188] [189] [190] [191] [192] [193] [194] [195] [196] [197] [198] . Importantly, the widespread use of high-throughput sequencing, PoC technologies, and advanced computational analysis tools may facilitate discovering and distinguishing these miRNAs.

HPV is the most common sexually transmitted infection worldwide. PLWH are at high risk of acquiring HPV infection and developing HPV-associated malignancies, independent of AIDS. Anal cancer incidence, though rare in the general population, has been rising significantly in PLWH. The lack of standard screening programs contributes to the increased incidence of anal cancer, and thus, there is a need for anal dysplasia screening and treatment in PLWH. The discovery of highly sensitive and specific biomarkers would enable the early detection of anal cancer and the improved survival of HIV-infected patients. There is a need for relevant biomarkers that could be integrated into clinical practice and thus, aid in the detection, diagnosis, and treatment of high-risk patients. miRNAs have become valuable tools for detection and treatment of many types of cancer. Given their deregulation and potentially significant role in HPV-related pathogenesis and in HIV infections, miRNAs may serve as diagnostic and prognostic biomarkers that can enhance HIV patients' outcomes and provide better management of the disease. Genome-wide profiling of miRNAs and validation of miRNA targets in tissue and blood samples of people infected with HIV and HR-HPV is important to establish miRNA expression signatures in this population and would help develop non-invasive miRNA therapeutic strategies for treatment of anal cancer. Targeted mRNAs of genes involved in apoptosis, T cell migration, and proliferation [192] miR-128a, and miR-3182 -Tat101-expressing Jurkat cells ---- [192] miR-132 + Tat-transfected astrocytes and neurons, astrocytes from Tat-transgenic mice, and HIV-infected astrocytes Involved in the direct neurotoxicity of Tat [193] miR-129, miR-135a, miR-181a, miR-495, miR-523, miR-524, miR-539, let-7 -U-87MG (astrocyte cell line), HEK 293T, and HeLa cells transfected with wild-type Tat Downregulation of β-catenin activity [194] miR-101 + BMVECs exposed to Tat C Decreased the expression of VEcadherin [195] miR-34a and miR-138 + Astrocytoma cell line A172 and rat primary astrocytes exposed to Tat Upregulated NF-κB and promoted activation of astrocytes [196] Nef miR-573 and miR-638 + Human monocytic U937 cells that stably expressed HIV-1 Nef

Altered several pathways involved in HIV pathogenesis [197] miR16-1, miR-18, miR-19a, miR-20a, miR-21, miR-27a, miR-29b, miR-125b, miR-146a, miR-146b-3p, miR-181a, miR-223, miR-570, miR-610 and miR-624 -Human monocytic U937 cells that stably expressed HIV-1 Nef

Altered several pathways involved in HIV pathogenesis [197] miR-17, miR-19a, miR-19b, miR-20a, miR26a, miR-28, miR-29a, miR-29b, miR-29c, miR-92a, miR-125b, miR-149, miR-150, miR-223, miR-324-5p, miR-378 and miR-382 + Nef exosomes Inhibited HIV replication [197] Vpr miR-942-5p + PEL cells Targeted IκBα and activation of NF-κB signalling [198] miR-711 + PEL cells Directly targeted Notch1 and reduced levels of IκBα transcript [198] "+": Upregulation; "-": Downregulation; miRNA: Micro ribonucleic acid; HIV: Human Immunodeficiency virus; HEK: Human embryonic kidney cells; BMVEC: Human brain microvascular endothelial cells; PEL: Primary effusion lymphoma; NF-κB: NF-kappaB inhibitor.

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Emerging biomarkers and clinical significance of HPV genotyping in prevention and management of cervical cancer

Pathogenic role of exosomes and microRNAs in HPV-mediated inflammation and cervical cancer: A review

microRNA-499a promotes the progression and chemoresistance of cervical cancer cells by targeting SOX6

Systematic Genome-wide Screening and Prediction of microRNAs in EBOV During the

Genome-wide computational prediction of miRNAs in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) revealed target genes involved in pulmonary vasculature and antiviral innate immunity

Circulating MicroRNA Biomarkers in Melanoma: Tools and Challenges in Personalised Medicine

Identification of potential microRNA panels for pancreatic cancer diagnosis using microarray datasets and bioinformatics methods

Validation of miRNAs as Breast Cancer Biomarkers with a

MicroRNAs Associated With Colon Cancer: New Potential Prognostic Markers and Targets for Therapy

A mathematical model for microRNA in lung cancer

Avenues Toward microRNA Detection In Vitro: A Review of Technical Advances and Challenges

MicroRNA amplification and detection technologies: opportunities and challenges for point of care diagnostics

Ultrasensitive detection of microRNAs by exponential isothermal amplification

Lateral flow nucleic acid biosensor for sensitive detection of microRNAs based on the dual amplification strategy of duplexspecific nuclease and hybridization chain reaction

Nanotechnology-based strategies for the detection and quantification of microRNA

A review on the electrochemical biosensors for determination of microRNAs

Rapid and sensitive microRNA detection with laminar flow-assisted dendritic amplification on power-free microfluidic chip

Microfluidic device for novel breast cancer screening by blood test using miRNA beacon probe

Exosomal RNA as biomarkers and the therapeutic potential of exosome vectors

Circulating microRNAs as stable blood-based markers for cancer detection

Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins

Non-Exosomal and Exosomal Circulatory MicroRNAs: Which Are More Valid as Biomarkers?

Circulating Exosomal miR-17-5p and miR-92a-3p Predict Pathologic Stage and Grade of Colorectal Cancer

An elevated expression of serum exosomal microRNA-191, -21, -451a of pancreatic neoplasm is considered to be efficient diagnostic marker

An epithelial organoid model with Langerhans cells for assessing virus-host interactions

Oral Mucosal Organoids as a Potential Platform for Personalized Cancer Therapy

Modeling Cell Communication in Cancer With Organoids: Making the Complex Simple

Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer

Up-regulation of miR-20a by HPV16 E6 exerts growth-promoting effects by targeting PDCD6 in cervical carcinoma cells

Human papillomavirus E6-regulated microRNA-20b promotes invasion in cervical cancer by targeting tissue inhibitor of metalloproteinase 2

Human papillomavirus type 16 E6 induces cervical cancer cell migration through the p53/microRNA-23b/urokinase-type plasminogen activator pathway

MiR-30c-2* negative regulated MTA-1 expression involved in metastasis and drug resistance of HPV-infected non-small cell lung cancer

Oncogenic HPV infection interrupts the expression of tumor-suppressive miR-34a through viral oncoprotein E6

HPV E6/p53 mediated down-regulation of miR-34a inhibits Warburg effect through targeting LDHA in cervical cancer

Glucocorticoid regulation of a novel HPV-E6-p53-miR-145 pathway modulates invasion and therapy resistance of cervical cancer cells

MicroRNA195 inhibits cell proliferation, migration and invasion by targeting defective in cullin neddylation 1 domain containing 1 in cervical cancer

Human papillomavirus type 16 reduces the expression of microRNA-218 in cervical carcinoma cells

MicroRNA-218 inhibits EMT, migration and invasion by targeting SFMBT1 and DCUN1D1 in cervical cancer

Utility of microRNAs and siRNAs in cervical carcinogenesis

MiR-375 Is Epigenetically Downregulated by HPV-16 E6 Mediated DNMT1 Upregulation and Modulates EMT of Cervical Cancer Cells by Suppressing lncRNA MALAT1

miR-2861 acts as a tumor suppressor via targeting EGFR/AKT2/CCND1 pathway in cervical cancer induced by human papillomavirus virus 16 E6

HPV 16 E7 inhibits OSCC cell proliferation, invasion, and metastasis by upregulating the expression of miR-20a

Regulator role of HPV E7 protein on miR-21 expression in cervical carcinoma cells and its functional implication

Up-Regulation of miR-21 Is Associated with Cervicitis and Human Papillomavirus Infection in Cervical Tissues

MicroRNA-27b up-regulated by human papillomavirus 16 E7 promotes proliferation and suppresses apoptosis by targeting pololike kinase2 in cervical cancer

miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition

miR-24 and miR-205 expression is dependent on HPV oncoprotein expression in keratinocytes

Cervical cancer subtypes harbouring integrated and/or episomal HPV16 portray distinct molecular phenotypes based on transcriptome profiling of mRNAs and miRNAs

miR-22 inhibits tumor growth and metastasis by targeting ATP citrate lyase: evidence in osteosarcoma, prostate cancer, cervical cancer and lung cancer

MiR-125a suppresses tumor growth, invasion and metastasis in cervical cancer by targeting STAT3

HPV16 E6 and E7 upregulate the histone lysine demethylase KDM2B through the c-MYC/miR-146a-5p axys

Human papillomaviruses modulate expression of microRNA 203 upon epithelial differentiation to control levels of p63 proteins

MicroRNA 203 expression in keratinocytes is dependent on regulation of p53 Levels by E6

Relationships of PBMC microRNA expression, plasma viral load, and CD4+ T-cell count in HIV-1-infected elite suppressors and viremic patients

MicroRNA profile changes in human immunodeficiency virus type 1 (HIV-1) seropositive individuals

Interplay between HIV-1 infection and host microRNAs

Are microRNAs Important Players in HIV-1 Infection?

Suppression of microRNAsilencing pathway by HIV-1 during virus replication

Two cellular microRNAs, miR-196b and miR-1290, contribute to HIV-1 Latency

MicroRNA regulation and its effects on cellular transcriptome in human immunodeficiency virus-1 (HIV-1) infected individuals with distinct viral load and CD4 cell counts

mircoRNA-3162-3p is a potential biomarker to identify new infections in HIV-1-infected patients

Differential regulation of the Let-7 family of microRNAs in CD4+ T cells alters IL-10 expression

Development and validation of plasma miRNA biomarker signature panel for the detection of early HIV-1 infection

HIV-1 Infection Dysregulates Cell Cycle Regulatory Protein p21 in CD4+ T Cells Through miR-20a and miR-106b Regulation

Changes in the cellular microRNA profile by the intracellular expression of HIV-1 Tat regulator: A potential mechanism for resistance to apoptosis and impaired proliferation in HIV-1 infected CD4+ T cells

HIV-1 Tat-shortened neurite outgrowth through regulation of microRNA-132 and its target gene expression

The inhibition of microRNAs by HIV-1 Tat suppresses beta catenin activity in astrocytes

HIV-1 Tat C modulates expression of miRNA-101 to suppress VE-cadherin in human brain microvascular endothelial cells

Tat-Mediated Induction of miRs-34a & -138 Promotes Astrocytic Activation via Downregulation of SIRT1: Implications for Aging in HAND

The HIV Nef protein modulates cellular and exosomal miRNA profiles in human monocytic cells

Upregulation of MicroRNA 711 Mediates HIV-1 Vpr Promotion of Kaposi's Sarcoma-Associated Herpesvirus Latency and Induction of Proproliferation and Pro-survival Cytokines by Targeting the Notch/NF-κB-Signaling Axis

The authors would like to thank Mr. Rawad Abdul Salam for his valued assistance in drawing the illustrations.