key: cord-0806402-fzhevc16 authors: Groves, Helen E.; Piché-Renaud, Pierre-Philippe; Peci, Adriana; Farrar, Daniel S.; Buckrell, Steven; Bancej, Christina; Sevenhuysen, Claire; Campigotto, Aaron; Gubbay, Jonathan B.; Morris, Shaun K. title: The impact of the COVID-19 pandemic on influenza, respiratory syncytial virus, and other seasonal respiratory virus circulation in Canada: A population-based study date: 2021-07-17 journal: Lancet Reg Health Am DOI: 10.1016/j.lana.2021.100015 sha: dc413d42dca4b0cd4df65eac34a04ad9694c58a3 doc_id: 806402 cord_uid: fzhevc16 Background The ongoing coronavirus disease 2019 (COVID-19) pandemic has resulted in implementation of public health measures worldwide to mitigate disease spread, including; travel restrictions, lockdowns, messaging on handwashing, use of face coverings and physical distancing. As the pandemic progresses, exceptional decreases in seasonal respiratory viruses are increasingly reported. We aimed to evaluate the impact of the pandemic on laboratory confirmed detection of seasonal non-SARS-CoV-2 respiratory viruses in Canada. Methods Epidemiologic data were obtained from the Canadian Respiratory Virus Detection Surveillance System. Weekly data from the week ending 30th August 2014 until the week ending the 13th March 2021 were analysed. We compared trends in laboratory detection and test volumes during the 2020/2021 season with pre-pandemic seasons from 2014 to 2019. Findings We observed a dramatically lower percentage of tests positive for all seasonal respiratory viruses during 2020-2021 compared to pre-pandemic seasons. For influenza A and B the percent positive decreased to 0•0015 and 0•0028 times that of pre-pandemic levels respectively and for RSV, the percent positive dropped to 0•0169 times that of pre-pandemic levels. Ongoing detection of enterovirus/rhinovirus occurred, with regional variation in the epidemic patterns and intensity. Interpretation We report an effective absence of the annual seasonal epidemic of most seasonal respiratory viruses in 2020/2021. This dramatic decrease is likely related to implementation of multi-layered public health measures during the pandemic. The impact of such measures may have relevance for public health practice in mitigating seasonal respiratory virus epidemics and for informing responses to future respiratory virus pandemics. Funding No additional funding source was required for this study. The emergence of coronavirus disease 2019 (COVID-19) has resulted in an unprecedented global pandemic leading to significant morbidity and mortality, particularly among older and vulnerable adult populations [1] . It has been more than one year since the pandemic first began and in this time, policy makers worldwide have implemented stringent mitigation effort s to reduce transmission of severe acute respiratory syndrome virus 2 (SARS-CoV-2). These measures have included the implementation of local and international travel restrictions; use of targeted lockdowns including stay-at-home orders and school closures; universal guidance on handwashing, physical distancing and, use of face coverings [ 2 , 3 ] . In Canada, restrictions on international travel with a 14-day quarantine for returning travellers who do not meet exemption criteria were introduced on 25 th March 2020 and these have remained in effect since [4] . Localised lockdown policies have also been implemented, with variation in region-specific stay-at-home orders and, school closures [5] . Previously, there was concern regarding the potential of increased healthcare burden from the dual impact of an ongoing COVID-19 pandemic in many countries coinciding with the seasonal influenza virus peak which causes significant annual morbidity and mortality [6] . Understanding of the impact of COVID-19-related transmission mitigation measures on the transmission of other respiratory viruses is limited. Increasingly, it is being recognised that during the COVID-19 pandemic, the traditional respiratory virus season has been significantly altered with notable decreases in the incidence of other seasonal respiratory viral infections [ 3 , 7 , 8 ] . In particular, significant decreases in influenza detection in the 2020 to 2021 season are being reported by surveillance data from many countries worldwide. In the United States, influenza-related hospitalisation rates are lower during the 2020/2021 season compared to any season since routine data collection began in 2005 and similarly, Australian influenza surveillance reported historically low activity levels from April 2020 onwards [ 9 , 10 ] . The reasons for this observed significant decrease are not yet fully understood and are likely related, at least in part, to the aforementioned COVID-19 mitigation strategies. The impact of the current COVID-19 pandemic on detection of influenza, respiratory syncytial virus and other seasonal respiratory viruses across all of Canada during the 2020-2021 season has not previously been reported. The objective of this study is to characterise the epidemiology of laboratory confirmed detection of influenza, respiratory syncytial virus, and other non-SARS-CoV-2 seasonal respiratory viruses across Canada before and during the COVID-19 pandemic. The study is a population-based observational study using Canada-wide laboratory surveillance data sources as detailed below. Data on non-SARS-CoV-2 virus detection were obtained from the Canada Respiratory Virus Detection Surveillance System [11] . This national surveillance is coordinated by the Public Health Agency of Canada (PHAC). Sentinel public health and hospital laboratories across Canada provide PHAC with weekly summaries of respiratory virus test results and test volumes. PHAC collates the data and delivers weekly publicly available updates. Reporting laboratories, include reference and public health laboratories for the Atlantic region, Province of Quebec, Province of Ontario, Prairies region, British Columbia and the Territories. For further details on laboratories included please refer to https://www.canada.ca/en/public-health/services/ surveillance/respiratory-virus-detections-canada.html . Weekly Respiratory Virus Detections data are collected continuously, with reports available from 2006. Data on SARS-CoV-2 cases in Canada were obtained from the Government of Canada Public Health Infobase [12] . Public Health Infobase data is co-ordinated and managed by PHAC. The number of new daily cases of COVID-19 (confirmed and probable) are established from the net change between what provinces and territories report to PHAC for the current day and for the previous reported day. Information on international and provincial travel restrictions and public health measures were obtained from the Government of Canada and Public Health Agency of Canada [ 13 , 14 ] . All reported data in this study contains information licensed under the Open Government Licence -Canada. All data included in this analysis was obtained from publicly available de-identified datasets and therefore additional ethical approval was not required. The study population included all respiratory virus tests conducted at sentinel reporting laboratories in Canada during the study period of 2014 to 2021 inclusive. The reporting laboratories provide limited information on participant demographics and no clinical information on cases (either positive or negative) is available. Limited available demographic information was not analysed for this study. In this study we analysed weekly data from the week ending 30 th August 2014 (epidemiological week 35) until the week ending the 13 th March 2021 (epidemiological week 10) inclusive. This study period was chosen to include five full respiratory virus seasons prior to 2020. For the purpose of the study, we defined a case of non-SARS-CoV-2 respiratory virus as any laboratoryconfirmed positive test for non-SARS-CoV-2 respiratory viruses reported to PHAC. Non-SARS-CoV-2 respiratory viruses included influenza, respiratory syncytial virus (RSV), parainfluenza viruses (PIV) types 1, 2, 3 and 4, adenovirus, human metapneumovirus (hMPV), enterovirus/rhinovirus, and seasonal coronaviruses. Seasonal coronavirus detection includes seasonal human coronaviruses HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1 and does not include human coronaviruses SARS-CoV, MERS-CoV, and SARS-CoV-2. Weekly percentage of tests positive for each virus was defined as the number of cases reported over the total number of tests reported for the epidemiologic week under surveillance, expressed as a percentage. For SARS-CoV-2 cases we analysed daily data from 31 st January 2020 to 13 th March 2021 inclusive. For the purposes of this study, we defined weekly SARS-CoV-2 cases as the number of confirmed or probable cases of COVID-19 reported to PHAC for each week under study. For greater detail on case definition, fluctuation in daily case reporting across provinces and territories and a full list of web sources used in the epidemiological data please refer to healthinfobase.canada.ca and the Government of Canada COVID-19 National Case Definitions [ 15 , 16 ] . The total study period included the 2014/2015 influenza season, defined as beginning at the week ending 30th August 2014 (epidemiological week 35) until the 2020/2021 influenza season, the week ending 13th March 2021 (epidemiological week 10). The baseline "pre-pandemic" period was defined as beginning from the week ending 30th August 2014 to the week ending 7th March 2020 inclusive. This period was selected to allow inclusion of five full seasons of baseline data until the global pandemic was called by the World Health Organization (WHO) on March 11, 2020 [17] . The pre-pandemic period was compared to the 2020/2021 season to date, which we defined as the week ending 29 th August 2020 (epidemiological week 35) to the week ending the 13 th March 2021 (epidemiological week 10) inclusive. An inter-season period was defined as beginning at the onset of the COVID-19 pandemic from the week ending 14 th March 2020 (epidemiological week 11) until the week ending 22 nd August 2020 (epidemiological week 34) inclusive. For primary comparative analysis the inter-season period was not included and to permit similar comparison with the prepandemic period, only epidemiological weeks 35 to 10 were included for each season (i.e. epidemiological weeks 11 to 34 each season were excluded). Data analysis was performed using GraphPad Prism 9.0.2 (Graph-Pad Software, LLC) and Stata version 16.1. Interrupted time series analyses [18] were performed to assess the difference in percent positivity for each virus of interest between the pre-pandemic period and the 2020/2021 season. Specifically, segmented negative binomial regression was used given the presence of over dispersion and each model included a) the number of positive tests; b) two pairs of Fourier sine-cosine terms; c) a binary variable denoting the time period as either pre-pandemic or 2020/2021 season; and d) a discrete variable denoting week of the study period to account for potential changes in respiratory virus testing over time; and e) an offset of the number of tests conducted. Rate ratios and 95% confidence intervals were generated between the pre-pandemic and 2020/2021 season. Sensitivity analyses including the inter-season period were also conducted. A p-value of < 0.05 was regarded as statistically significant. The number of positive laboratory results was summarized by virus type using line graphs. Data on rhinovirus/enterovirus testing in the Province of Quebec, data on PIV, adenovirus, hMPV and rhinovirus testing in the Yukon territory and data on seasonal coronaviruses testing in Newfoundland were not available throughout the entire study period. One sentinel laboratory, St. Joseph's Healthcare in Hamilton, Ontario, has been added to those providing surveillance data over the study period and began reporting data on the week ending 23 rd November 2019. Laboratories in the Territories region did not report data in the 2014/2015 season. One sentinel laboratory, Saskatoon laboratory, Saskatchewan, stopped reporting during the study period from the week ending 15 th February 2020. To identify the impact of these reporting differences, analysis was repeated excluding the data from St. Joseph's Healthcare in Hamilton, the Territories and Saskatoon Laboratories. This study is reported according to the STROBE guideline for observational studies. No funding source was required for completion of this work. A total of 6,722,859 tests for non-SARS-CoV-2 respiratory viruses (1,634,574 influenza; 541,699 enterovirus/rhinovirus; 1,468,261 RSV; 839,583 PIV; 607,797 seasonal coronaviruses; 793,990 hMPV, and 836,955 adenovirus) were recorded over the pre-pandemic study period from sentinel laboratories across Canada. In the 2020/2021 season, a total of 1,267,545 tests for non-SARS-CoV-2 respiratory viruses were recorded from sentinel laboratories across Canada (372,823 influenza; 129,298 enterovirus/rhinovirus; 257,799 RSV; 133,001 PIV; 109,873 seasonal coronaviruses; 132,772 hMPV, and 131,979 adenovirus). The average weekly number of combined laboratory tests for all non-SARS-CoV-2 respiratory viruses performed was reported as 28,039 (range from 7,383 to 63,795) for the pre-pandemic period (excluding epidemiological weeks 11 to 34) and 43,708 (range from 27,597 to 77,110) for the 2020/2021 season. Table 1 details average weekly testing for each virus of interest. By comparison, for the period from the week ending 29 th August 2020 (epidemiological week 35) to the week ending the 13 th March 2021 (epidemiological week 10) inclusive., 20,155,105 SARS-CoV-2 tests were reported to have been performed at reporting laboratories throughout Canada. We observed dramatically lower detection levels of all non-SARS-CoV-2 respiratory viruses during the 2020-2021 season compared to pre-pandemic levels ( table 1 , Figure 1, 2 and 3 ). This observed decrease did not seem to be related to a drop in overall numbers of tests for these viruses (supplemental figures S1 and S4). Pre-pandemic period begins the week ending 30 th August 2014 through to the week ending 7 th March 2020 inclusive. The 2020/2021 season was defined as beginning the week ending 29 th August 2020 to week ending 13 th March 2021 inclusive. Comparative analysis excluded the same inter-season period (epidemiological week 11 to week 34) for all pre-pandemic years as well as for the 2020/20201 season. * Rate ratio and p-values calculated using interrupted time series to assess the difference in percent positivity for each virus of interest between the prepandemic period and the 2020/2021 season period. as described in methods. This table is based on weekly count data. Abbreviations: RSV = Respiratory syncytial virus, PIV = parainfluenza virus (includes combined type 1,2,3 and other types), hMPV = human metapneumovirus. * * Coronavirus excludes human coronaviruses SARS-CoV, MERS-CoV and SARS-CoV-2; Includes seasonal human coronaviruses HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1. Note data on rhinovirus/enterovirus testing in the Province of Quebec, data on PIV, adenovirus, hMPV and rhinovirus testing in the Yukon territory and data on seasonal coronaviruses testing in Newfoundland were not available for any week throughout the entire study period. One sentinel laboratory, St. Joseph's Healthcare in Hamilton, Ontario, has been added to those providing surveillance data over the study period and began reporting data on the week ending 23 rd November 2019. Laboratories in the Prairies region did not report data in the 2014/2015 season. One sentinel laboratory, Saskatoon laboratory, Saskatchewan, stopped reporting during the study period from the week ending 15 th February 2020. Analysis excluding the data from St. Joseph's Healthcare in Hamilton, the Territories and Saskatoon Laboratory did not appreciably change the results, with no change in p-values noted and minimal difference in magnitude any of the rate ratios (see table S1 ). comparison of the percentage of tests positive for the 2020/2021 season with pre-pandemic seasons also showed significant decreases. The percentage of tests with positive results for PIV and adenovirus decreased to 0 • 0019 (0 • 014-0 • 025) and 0 • 234 (0 • 200-0 • 273) times that of pre-pandemic levels respectively, for hMPV this decreased to 0 • 038 (0 • 024-0 • 059) times that of pre-pandemic levels, for enterovirus/rhinovirus and seasonal coronaviruses this decreased to 0 • 531 (0 • 480-0.593) and 0 • 028 (0 • 019-0 • 041) times that of pre-pandemic levels respectively. Importantly, at the time of these observed decreases in percentage of positive tests for non-SARS-CoV-2 respiratory viruses, ongoing cases of SARS-CoV-2 were reported with an initial peak in cases in April 2020 followed by a larger second peak in January 2021 ( Figure 1 b,c) . Interestingly, higher (although still attenuated) ongoing detection of enterovirus/rhinovirus was reported during the 2020/2021 season versus other non-SARS-CoV-2 respiratory viruses, with average weekly percentage of tests positive of 8 • 5%, versus less than 1% respectively ( table 1 , Sensitivity analysis excluding the data from St. Joseph's Healthcare in Hamilton, the Territories and Saskatoon Laboratory there was no appreciable change in the results, with no change in pvalues noted and minimal difference in magnitude any of the rate ratios (supplementary table S1). In sensitivity analyses including the inter-season periods, steep declines in weekly percentage of tests positive were still detected, though rate ratios for most viruses were more conservative primarily due to ongoing detection during March and April 2020 (supplementary table S2). This study is the first Canada-wide study to examine the impact of the COVID-19 pandemic on the detection of both influenza and non-influenza seasonal respiratory viruses during the 2020-2021 season. In this extensive study of public health and hospital microbiology laboratory data across Canada, we demonstrate a significant change in the seasonal variation of respiratory viral infections detected during the current COVID-19 pandemic. With the exception of ongoing enterovirus/rhinovirus detection, the usual seasonal peak in positive laboratory tests for common respiratory viruses, including non-SARS-CoV-2 coronaviruses was effectively absent during the 2020-2021 season. This decrease in detection of non-SARS-CoV-2 respiratory viruses is consistent with studies from the United States and United Kingdom showing an early termination of the 2019/2020 influenza season and drop in detection of other viruses in the early phases of the pandemic [ 3 , 19 ] . Similarly, a number of studies in Japan, Taiwan, Korea and Thailand demonstrated a marked decline in influenza virus detection in the early phases of the COVID-19 pandemic [20] [21] [22] [23] [24] . As the pandemic has progressed and national lockdowns continued, studies of the respiratory virus season in the southern hemisphere from New Zealand and Australia demonstrated absence of the usual winter influenza virus seasonal peak and a marked reduction of other respiratory viruses [ 25 , 26 ] . Typically in the Northern hemisphere, influenza, RSV and non-SARS-CoV-2 human coronaviruses are noted to peak during winter months, with adenovirus detection occurring throughout the year, peaks of parainfluenza viruses occurring in non-winter months and human metapneumoviruses and rhinoviruses being mainly detected during spring and fall seasons [27] . The observed decrease in almost all seasonal respiratory viruses during the COVID-19 pandemic is likely related in large part to the implementation of multiple public health interventions, including restriction of international travel, hand-washing, wearing of face masks, school closures and stay-at-home orders. We note that at the same time of no detection of circulating seasonal coronaviruses, there were ongoing increases in cases of COVID-19 due to continuing SARS-CoV-2 transmission, which may reflect the difference in population susceptibility to the novel coronavirus. Seasonal human coronavirus antibodies are not associated with protection against SARS-CoV-2 infection [28] . Whether antibodies to SARS-CoV-2 provide any cross-protection against seasonal coronaviruses remains unknown. We also reported continued detection of enterovirus/rhinoviruses during the 2020-2021 season. While this may suggest ongoing circulation of enterovirus/rhinoviruses despite public health measures to control COVID-19, variation in regional multi-layered public health measures may account for this continued circulation. Other studies have reported similar continued enterovirus/rhinovirus detections despite implementation of public health measures to reduce the spread of SARS-CoV-2. In a recent study of children in New South Wales, Australia, despite significant decreases from the expected RSV seasonal peak between April and June 2020, an uptick in rhinovirus detections was noted in June 2020 [26] . Similarly, in a recent study in New Zealand, based on hospital-based severe acute respiratory surveillance data, between May and September 2020, marked reductions in rhinovirus levels were observed on the introduction of initial lockdown measures, however on easing restrictions a notable significant increase in rhinovirus-associated incidence rates was observed compared to other respiratory viruses [25] . This potential for rhinovirus resurgence is important for the interpretation of syndromic surveillance models for COVID-19 as there is considerable overlap in clinical symptoms for even mild enterovirus/rhinovirus SARS-CoV-2 [29] . An apparent increase in rhinovirus detection on reduction of public isolation measures has also been reported in hospitalised adults in the UK following the re-opening of schools [30] . Likewise, in Hong Kong, after the re-opening of schools and childcare centres, a large number of outbreaks of acute upper respiratory tract infections (URTIs) occurred and on laboratory testing rhinovirus/enterovirus infections were identified [31] . The reasons for this reported resurgence of rhinovirus compared to other respiratory viruses are unclear. Rhinovirus is a non-enveloped virus that may be less susceptible to inactivation by handwashing [26] . In addition, enteroviruses have the potential for fecal/oral transmission and it is possible that differences in the mechanism of enterovirus/rhinovirus transmission compared to other seasonal respiratory viruses mean more stringent public health measures are required to fully reduce transmission. On provincespecific analysis of enterovirus/rhinovirus testing percentage positivity in Canada, regional variation during the 2020/2021 season was present. Higher percent positivity was seen in the Territo- ries and Atlantic regions with reduced peaks compared to historical baseline observed for Ontario, British Columbia and The Prairies. This may reflect differences in regional implementation of public health measures, such as later introduction of compulsory facemask guidance, as well as differences in return to in-person classes for children and indoor gathering regulations [32] . It is also possible that provincial/regional differences in SARS-CoV-2 activity may have played a role. In addition, rhinovirus infections are more likely to be symptomatic in children [33] , who may be differentially impacted by regional variation in public health measures such as school closures and masking mandates. Due to the absence of age-specific data in this study we are unable to assess the role of circulating enterovirus/rhinovirus in the paediatric population specifically. Overall, our results are consistent with a growing body of work indicating strict public health measures, such as regional lockdowns, border closures, handwashing and facemask wearing may be effective in significantly reducing the spread of epidemic respiratory viruses. However, what is notable in this study is the observed dramatic decrease in non-SARS-CoV-2 respiratory viruses including seasonal coronaviruses, despite ongoing detection of SARS-CoV-2 during the 2020/2021 winter season. This may reflect increased transmissibility of SARS-CoV-2 in comparison to other respiratory viruses, due to lack of preceding population immunity, such that it outcompetes seasonal respiratory viruses which are being more readily impacted by the measures implemented to mitigate SARS-CoV-2 transmission. It is difficult at this time to determine whether there is also potential impact of viral-viral interactions between SARS-CoV-2 and other respiratory viruses contributing to the observed decline in circulation. The potential for interaction between respiratory viruses has been speculated by pre-vious modelling studies, such as work by Nickbakhsh et al. who found both positive and negative respiratory viruses interaction at population and individual host level and suggested that viral interference may account for reduced frequency of rhinovirus infections during influenza seasons [34] . Nickbakhsh et al. further suggested such viral interference may potentially occur via interferonmediated mechanisms. This concept is supported by recent in vitro work in differentiated airway epithelial cultures, demonstrating upregulation of interferon stimulated gene expression following rhinovirus infection, leading to significant inhibition of subsequent influenza A virus infection [35] . Another potential explanation for the apparent dramatic decrease in seasonal respiratory viruses despite ongoing SARS-CoV-2 detection is a possible reduction in testing and/or laboratory reporting for non-SARS-CoV-2 respiratory viruses. We recognise one potential limitation of this study is that laboratories and hospitals may have become overburdened with implementing large scale testing for SARS-CoV-2 leading to changes in testing and reporting mechanisms for seasonal respiratory viruses compared to previous years. Although, importantly, we did not note a decrease in the total number of laboratory tests performed for each of the seasonal respiratory viruses studied during the 2020/2021 season compared to pre-pandemic. Indeed, early in the pandemic an apparent increase in the total number of tests performed for each virus of interest was observed (supplemental figure S4) . Thereafter, towards the end of 2020, the total number of non-SARS-CoV-2 tests performed decreased simultaneous to increased testing for SARS-CoV-2. It is likely that, compared to previous seasons, variation in testing practices by regional and local laboratories, such as increased periodic community testing, combined influenza/SARS-CoV-2 testing and increased testing for influenza and other seasonal respi- ratory viruses may have occurred during the 2020/2021 season. Moreover, it is worth noting that approaches for population testing of SARS-CoV-2 and non-SARS-CoV-2 viruses also differed, with testing of SARS-CoV-2 being much more community-based in comparison to other respiratory virus testing. There are a number of potential limitations of this study. Firstly, this is a retrospective observational study and it is difficult to identify whether the described decrease is as a direct result of multilayered public health strategies to mitigate spread of SARS-CoV-2 or other unidentified factors. Strategies were added in close proximity and targeted specific populations, via for example school and workplace closures, at different times. These differing measures were implemented with substantial regional variation across Canadian provinces, such that identifying individual key factors contributing to the overall observed decrease in non-SARS-CoV-2 respiratory virus detection is not possible in this study. In addition, baseline demographic and clinical comorbidity data were not available and accordingly these factors could not be adjusted for in the comparative analysis. This study used data from the Canadian Respiratory Virus Detections Surveillance System, which relies on reporting of results from individual laboratories. Changes in testing policies and eligibility at reporting laboratories may have led to differences in detection of non-SARS-CoV-2 respiratory viruses during the pandemic. Laboratory reporting remained consistent throughout the pandemic for most reporting laboratories and as detailed above. Analysis excluding data from sentinel laboratories with variation in reporting practice during the pandemic compared to pre-pandemic resulted in no significant change in the time-series or rate ratio results. Other changes in testing practices such as the introduction of COVID-19 assessment centres throughout Canada could have an impact on respiratory viruses detection during the pandemic. Furthermore, differences in health-seeking behaviour by individuals during the pandemic may also have altered the detection of non-SARS-CoV-2 respiratory viruses. For instance, in light of the concern for COVID-19, individuals with respiratory symptoms may have been more likely to attend for specific SARS-CoV-2 respiratory virus testing at designated testing centres rather than to receive testing for other respiratory viruses through usual mechanisms. This is likely to explain in part the higher numbers of SARS-CoV-2 tests and detections seen during the 2020-2021 respiratory virus season and may account for some of the differences observed in non-SARS-CoV-2 respiratory virus detection over the same period (see supplementary figure S4 ). In conclusion, in this study we report an effective absence of the annual seasonal epidemic for most non-SARS-CoV-2 respiratory viruses in Canada in 2020/2021 respiratory virus season. The reasons for this dramatic decrease are not yet clear and may reflect a number of different factors. It is likely the implementation of stringent measures to reduce the spread of SARS-CoV-2 also led to significant decreases in transmission of other respiratory viruses. However, the role of viral displacement and interference by SARS-CoV-2 is not yet known. In addition, the concept of a rebound in seasonal respiratory virus levels after relaxation of lockdown measures warrants further consideration. It is unclear whether the lack of exposure to non-SARS-CoV-2 respiratory viruses during the COVID-19 pandemic might result in larger outbreaks of other respiratory viral illnesses on easing of current public health measures in Canada. The absence of seasonal RSV and influenza epidemics also has potential implications for delivery of both palivizumab and influenza seasonal vaccination programs. Understanding the mechanisms behind the observed decreases in seasonal respiratory viruses is therefore of great importance and may benefit public health practice for mitigating seasonal respiratory virus epidemics and for informing responses to future respiratory virus pandemics. HEG: conceptualisation, data collection and verification of underlying data, formal data analysis, methodology, visualisation, Dr. Groves reports personal fees from Honoraria received from Abbvie for education meeting presentation, outside the submitted work. Dr. Piché-Renaud reports grants from Pfizer Global Medical Grants (Competitive grant program, investigator-led), outside the submitted work. Dr. Peci has nothing to disclose. Mr. Farrar has nothing to disclose. Mr. Buckrell has nothing to disclose. Dr. Bancej has nothing to disclose. Dr. Sevenhuysen has nothing to disclose. Dr. Campigotto has nothing to disclose. Dr. Gubbay has nothing to disclose. 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Anonymised raw data used to produce all the analyses, figures and tables for this paper are publically available for anyone who wishes to access the data. This data is available at the time of publication from the Government of Canada website via the links detailed in the methods section above. Details of the conditions for use of this open dataset are available on the Government of Canada website. Supplementary material associated with this article can be found, in the online version, at doi:10.1016/j.lana.2021.10 0 015 .