key: cord-0813247-i45wly4s authors: Marzook, N. Bishara; Latham, Sharissa L.; Lynn, Helena; Mckenzie, Christopher; Chaponnier, Christine; Grau, Georges E.; Newsome, Timothy P. title: Divergent roles of β‐ and γ‐actin isoforms during spread of vaccinia virus date: 2017-03-17 journal: Cytoskeleton (Hoboken) DOI: 10.1002/cm.21356 sha: 4341256921ce1b602b3a31db091950265fe573c4 doc_id: 813247 cord_uid: i45wly4s Actin is a major component of the cytoskeleton and is present as two isoforms in non‐muscle cells: β‐ and γ‐cytoplasmic actin. These isoforms are strikingly conserved, differing by only four N‐terminal amino acids. During spread from infected cells, vaccinia virus (VACV) particles induce localized actin nucleation that propel virus to surrounding cells and facilitate cell‐to‐cell spread of infection. Here we show that virus‐tipped actin comets are composed of β‐ and γ‐actin. We employed isoform‐specific siRNA knockdown to examine the role of the two isoforms in VACV‐induced actin comets. Despite the high level of similarity between the actin isoforms, and their colocalization, VACV‐induced actin nucleation was dependent exclusively on β‐actin. Knockdown of β‐actin led to a reduction in the release of virus from infected cells, a phenotype dependent on virus‐induced Arp2/3 complex activity. We suggest that local concentrations of actin isoforms may regulate the activity of cellular actin nucleator complexes. Mutant strains unable to undergo actin-based motility are attenuated in whole-animal mouse infection models and deficient in cell-tocell spread in vitro (Parkinson and Smith, 1994; Wolffe, Isaacs, & Moss, 1993; Wolffe, Katz, Weisberg, & Moss, 1997; Wolffe et al., 1998) . It is likely that actin-based motility contributes to virus spread via a variety of mechanisms. For example, the repulsion of superinfecting virions allows nonreplicative transmission across early stage-infected cells, thereby increasing the overall rate of cell-to-cell spread (Doceul, Hollinshead, van der Linden, & Smith, 2010) . It has also been observed that when VACV-induced actin nucleation is abrogated, WV remain trapped at the cell surface in pits (Horsington, Lynn, Turnbull, Cheng, Braet, Diefenbach, . . . Newsome, 2013) , indicating that actin nucleation provides a force necessary to resolve exocytosis of virus particles. Actin nucleation is instigated by the VACV type Ib transmembrane protein A36 that resides in the outer membrane of WV. Following fusion of this outer WV membrane with the plasma membrane, A36 becomes phosphorylated at Tyr112 and Tyr132 by the action of Srcand Abl-family tyrosine kinases (Frischknecht, Cudmore, Moreau, Reckmann, Rottger, & Way, 1999a; Newsome, Scaplehorn, & Way, 2004; Newsome, Weisswange, Frischknecht, & Way, 2006; Reeves, Bommarius, Lebeis, McNulty, Christensen, Swimm, . . . Kalman, 2005) . Phosphorylation of A36 leads to the recruitment of a host of cellular proteins, the core cascade comprising of Nck, WIP/WIRE and N-WASP, which act to stabilize and activate the Arp2/3 complex at the cytoplasmic surface of EV (Moreau et al., 2000; Snapper, Takeshima, Anton, Liu, Thomas, Nguyen, . . . Alt, 2001) . Optimal virus-induced actin nucleation also utilizes other host factors such as Grb2, FHOD1, casein kinase 2, clathrin, the clathrin adaptor AP2, and is accompanied by activation of the small Rho GTPases Cdc42 and Rac1 (Alvarez and Agaisse, 2013; Alvarez and Agaisse, 2014; Humphries, Dodding, Barry, Collinson, Durkin, & Way, 2012; Scaplehorn, Holmstrom, Moreau, Frischknecht, Reckmann, & Way, 2002) . The critical signal output that results from A36 tyrosine phosphorylation is actin nucleation, and drug inhibition of actin dynamics during viral infection phenocopies, to a large extent, the effects of genetic ablation of A36 or substitution of the Tyr112 and Tyr132 to phenylalanines (VACV-A36 YdF ) (Horsington et al., 2013) . The de novo nucleation of new actin filaments by the Arp2/3 complex, during normal cellular function or during VACV infection, stimulates robust actin polymerization by increasing the local concentration of free plus ends. Actin filaments are composed of actin monomers that are expressed from multiple loci that give rise to six highly conserved actin isoforms: two striated muscle (a-skeletal and a-cardiac), two smooth muscle (a-and g-) and two cytoplasmic (b-and g-) actins (Vandekerckhove and Weber, 1978b) . The muscle isoforms exhibit tissue specific expression, while the band g-cytoplasmic actins (CYA) are the most abundant in nonmuscle cells (Rubenstein, 1990) and often exist in 2:1 ratio in epithelial cell lines like HeLa and chicken embryo fibroblasts (Khaitlina, 2007) , the cell types commonly used to study VACV in vitro and cell types commonly targeted by orthopoxviruses during pathogenesis (Johnston and McFadden, 2004; McFadden, 2005; Smith and Kotwal, 2002; Werden, Rahman, & McFadden, 2008) . Despite their surprisingly similar sequence homologies [only differing by four amino acids; (Vandekerckhove and Weber, 1978a) ], recent reports have revealed that band g-CYA have different functions and display distinct subcellular localization patterns (Artman, Dormoy-Raclet, von Roretz, & Gallouzi, 2014; Dugina, Zwaenepoel, Gabbiani, Clement, & Chaponnier, 2009; Joseph, Srivastava, & Pfister, 2014; Pasquier, Tuset, Sinnappan, Carnell, Macmillan, & Kavallaris, 2015) . b-actin is predominantly found in basal areas of the cell, cell-cell contacts and stress fibres, while g-CYA is more apically abundant, and concentrated at peripheral structures such as lamellipodia and the leading edge of motile cells (Chaponnier and Gabbiani, 2004; Dugina et al., 2009] . Differential localization may contribute to the functional diversity of these isoforms, as g-CYA has been shown to be specifically required for directional cell migration, while b-CYA contributes to cell attachment and microparticle formation (Latham, Chaponnier, Dugina, Couraud, Grau, & Combes, 2013) . The aim of this study was to determine if there are isoform-specific functions of band g-CYA during the well-characterized phenomenon of VACV-induced actin nucleation. 2 | R E S U L T S 2.1 | VACV comets comprise both band c-CYA Given the differential distributions of band g-CYA in a cell, our first aim was to determine the actin isoform composition of VACV-induced actin 'comets'. Actin comets generated by a subpopulation of egressing virus particles are evident below the cell surface of VACV infected cells at 7-9 hr postinfection (hpi; Cudmore et al., 1995; Smith and Law, 2004) . Immunofluorescence assays (IFA) of siRNA-treated cells stained with anti-b-CYA and anti-g-CYA specific antibodies revealed the localization of both CYA isoforms in the cytoskeleton ( Figure 1a ) and VACV-induced actin comets in endothelial hCMEC/D3 cells ( Figure 1b ). VACV-induced actin comets are readily imaged at the level of the substrate due to the low z-axis profile of adherent cells. b-CYA preferentially localizes to the basal region, while g-CYA preferentially localizes to apical regions of HSCF cells (Dugina et al., 2009) . We therefore examined z-sections of infected cells to determine if the preference of actin isoforms along the apical-basal axis was reflected in their localization to actin comets on the apical or basal membrane. A z-stack of images of infected cells (Figure 1c ) with VACV-induced actin comets ( Figure 1d ) was taken via confocal microscopy. These data led us to conclude that VACV-associated actin comets are composed of both CYA isoforms irrespective of their basal or apical localization. Since both CYAs were reported to not only have different localizations, but also different functions within a cell (Dugina et al., 2009) , the next step was to validate siRNA-mediated depletion of individual actin isoforms that could be used to assay VACV actin-based motility. To this end, we established knockdown of b-CYA and g-CYA in HeLa, GBM MARZOOK ET AL. | 171 (glioblastoma) and hCMEC/D3 cell lines. Treatment of HeLa, GBM and hCMEC/D3 cells resulted in isoform-specific knockdown when compared to the scrambled siRNA condition (Figure 2a ). Densitometry analysis showed that b-CYA knockdown caused a significant reduction of b-CYA to about 31, 8, and 44% compared with levels in the scrambled siRNA control in HeLa, GBM, and hCMEC/D3 cells, respectively (p < .05, n 2; Figure 2b ). g-CYA siRNA treatment produced a comparatively less efficient knockdown of g-CYA to about 52, 35, and 55% in HeLa, GBM, and hCMEC/D3 cells, respectively (p < .05, n 2; Figure 2b ). Interestingly, knockdown of b-CYA led to a corresponding significant increase in g-CYA in HeLa and GBM cell types. This suggests that under knockdown conditions, cells overexpress one isoform to compensate for loss of the other. This has not been observed for CYA isoforms in other studies, either using the same cocktail of isoform-specific siRNA (Dugina et al., 2009) or using only one of those sequences (Pasquier et al., 2015) . This could be due to their use of different cell types, varying doses of siRNA or incubation times, and/or the use of only a single CYA-specific siRNA in one of the studies. The use of a pan-actin antibody which detects both cytoplasmic isoforms revealed that there is a compensatory effect of actin isoform expression during either b-CYA or g-CYA knockdown in HeLa cells, as there is no significant difference in total actin levels under either knockdown condition (Supporting Information Fig. S1 ). This is supported by observations from other studies where neither isoform knockdown significantly affected the total level of actin in HSCF cells (Dugina et al., 2009 ) and A549 human lung epithelial cells (Lechuga, Baranwal, Li, Naydenov, Kuemmerle, Dugina, . . . Ivanov, 2014) . GBM cells, however, show significantly reduced levels of total actin expression during g-CYA knockdown (Supporting Information Fig. S1 ). Since both CYAs were reported to not only have different localizations, but also different functions within a cell (Dugina et al., 2009) , the next step was to determine if there was a distinct role for either CYA in VACV-induced actin comets. hCMEC/D3 cells were treated with bor g-CYA-specific siRNA for 72 hr before being infected with VACV-WR and fixed 8 hpi. IFA of siRNA-treated cells stained with anti-b-CYA and anti-g-CYA specific antibodies (Dugina et al., 2009) significantly reduced in the presence of their respective siRNA, as shown by IFA (Figure 3a ). While bor g-CYA-specific siRNA have previously been shown to cause incomplete knockdown of their respective targets, it was still sufficient to cause significant changes to cell morphology and behavior (Dugina et al., 2009) . As with the control cells from Figure 1 , both b-CYA and g-CYA were detected in virusassociated actin comets in the scrambled siRNA-treated cells ( Figure 1b ). In g-CYA knockdown cells, g-CYA was greatly reduced and b-CYA was concentrated at VACV-induced actin tails (Figure 3b Figure 4c) . Surprisingly, knockdown of g-CYA elicited a two-fold increase in virus motility (0.17 lm/s compared with 0.8 lm/s). In summary, we observed that actin comets produced under b-CYA knockdown are shorter although their speeds remain the same relative to the controls. There was no significant difference in length of actin comets produced by g-CYA knockdown and scrambled siRNA-treated HeLa cells. This implies that while b-CYA is necessary for the initiation of VACV comets, the ratio of b-CYA to g-CYA in a cell may determine the speed of VACV motility, whereby the presence of g-CYA in the actin comet has a moderating effect on virus speed. Since a clear attenuation of VACV-induced actin nucleation during b-CYA knockdown was evident, we next determined its effect on virus release, which is dependent on actin nucleation (Horsington et al., 2013) . HeLa cells under either CYA knockdown were infected with either the vaccinia reference strain VACV-WR, or the mutant strain VACV-A36 YdF . VACV-A36 YdF contains point mutations of the two tyrosine residues in A36R involved in recruiting actin polymerisation machinery, rendering A36 incapable of being tyrosine phosphorylated. The mutant virus is therefore unable to induce actin comets (Rietdorf et al., 2001 ) and a subsequent reduction in release of extracellular enveloped virus (EEV) from the cell is observed (Horsington et al., 2013) . Supernatants from infected cells were collected and plaque assays were performed to determine the infectious EEV titre of each condition ( Figure 5a ). In cells infected by VACV-WR, EEV release into the supernatant (measured in plaque forming units or pfu/mL) was significantly reduced under b-CYA knockdown. These results are consistent with our previous observations that disrupting actin-based motility leads to a corresponding reduction in EEV release. Cells infected by VACV-A36 YdF showed greatly reduced EEV release when compared with VACV-WR as has been previously reported (Horsington et al., 2013) . However, no difference in EEV release of VACV-A36 YdF was observed under either CYA knockdown condition. All phenotypes associated with actin-based motility are ablated in VACV-A36 YdF , and b-CYA knockdown does not exacerbate defects in EEV release in a VACV-A36 YdF . This suggests that b-CYA also disrupts EEV release through actin-based motility rather than other WV protein interactions known to affect EEV release (Smith, Vanderplasschen, & Law, 2002) . | 175 (Horsington et al., 2013; Rodger and Smith, 2002; Zhang, Wilcock, & Smith, 2000] , we aimed to test how b-CYA knockdown in cells might affect VACV plaque size or morphology. GBM cells (selected over HeLa due to their amenability to forming monolayers) were subjected to either CYA knockdown and allowed to near confluence, at which point a plaque assay was conducted with VACV-WR. Three days post infection, plaque assays were fixed and the diameter of plaques was measured (Figure 5e ). Confounding expectations, plaques produced by VACV-WR treated with b-CYA knockdown were significantly larger than those compared to plaques produced in cells under g-CYA knockdown or treated with scrambled siRNA. These results indicate that knockdown of b-CYA produces a complex phenotype and that other mechanisms may compensate for the expected reduction in plaque size elicited by the reduction in actin-based motility. Having uncovered a strict requirement for b-CYA for the efficient formation of VACV-induced actin comets, we wanted to further define FIG URE 4 Effects of CYA knockdown on VACV comet phenotype. (a) hCMEC/D3 cells were treated with the respective siRNA for 72 hr, followed by VACV-WR infection at an MOI > 5. Cells were fixed 8 hpi and stained for F-actin and the number of actin tails per cell was enumerated ('***': p < .001; n 5 40 cells for each treatment, with 3 replicate experiments performed). (b) hCMEC/D3 (D1) and HeLa (D2) cell lines were treated with the siRNA as indicated for 72 hr and infected with VACV-WR at an MOI > 3. Cells were fixed 8 hpi, followed by staining for F-actin and viral envelope proteins. Actin tail lengths were measured using ImageJ image-analysing software and graphs created using GraphPad PRISM, with nonparametric t-tests used to determine significance in differences between parameters (n 5 60 tails each). (c) Speeds of VACV actin comets were calculated using FIJI (ver. 2.0.0) image-analyzing software from live-cell imaging experiments and statistical analyses were carried out using GraphPad PRISM (ver. 6 for Mac OSX), with one-way ANOVA and Tukey's multiple comparison tests. "ns": p > .05, "*": p .05, "***": p .001, "****": p .0001. the mechanism. N-WASP is an actin nucleation-promoting factor that is recruited to the site of actin comet formation during VACV egress (Frischknecht et al., 1999b; Moreau et al., 2000) . The VCA domain of N-WASP recruits the Arp2/3 complex and two actin monomers, branching off from an existing actin filament. If the VCA domain were to have a preference for the b-CYA isoform, that would explain the dependence on b-CYA for actin-based virus transport. Two bacterial expression plasmids were constructed: one expressing a glutathione Stransferase (GST)-tagged VCA domain and another expressing a GSTtagged VCA domain containing two arginine-alanine substitutions in its actin-binding region, VCA-R410A/R438A (called VCA-RA/RA), which effectively abrogate actin binding (Co, Wong, Gierke, Chang, & Taunton, 2007) . GST-tagged VCA proteins were expressed in bacteria, purified and used to enrich for actin by passing cell lysates over the bound, immobilized protein and probing for actin isoforms. As expected, actin bound to GST-VCA and did not bind to the GST-VCA RA/RA ( Figure 6a ). The next step was to examine whether the actin that bound to the VCA domain was specifically one CYA isoform. Both anti-b-CYA and anti-g-CYA antibodies bound to an immunoblot of cell lysates passed over GST-VCA protein (Figure 6b) , suggesting that the VCA domain does not specifically bind to one CYA over the other. There has been increasing interest in the roles of the two CYA isoforms, b-CYA and g-CYA, as two distinct proteins with differing localizations and functions despite their high homology and overlapping expression. For example, while b-CYA localises to stress fibres and cellcell contacts, g-CYA is abundant in lamellipodial structures and is important for cell motility (Dugina et al., 2009) . Studies have identified isoform-specific functions during multiple cellular processes (Artman et al., 2014; Hajkova, Nyman, Lindberg, & Karlsson, 2000; Joseph et al., 2014; Pasquier et al., 2015) , as well as distinct requirements in the replication cycle of viruses like coronaviruses (Wang, Fang, Xiao, Chen, Tam, & Liu, 2009) While our data support a role for b-CYA knockdown in the release of EEV through actin nucleation, we also found that it did not disrupt the intracellular movement of enveloped virus particles to the cell periphery. This is as expected, as both the generation of intracellular enveloped VACV, as well as their transport to the cell periphery, is independent from release of EEV particles (Horsington et al., 2013; Lynn, Horsington, Ter, Han, Chew, Diefenbach, . . . Newsome, 2012; Payne and Kristensson, 1982; Ward and Moss, 2001) . difference in the number of virus particles either being released or being transported to the cell periphery under g-CYA depletion. As the major effects observed by Po'uha et al (2013) were a decrease in microtubule shortening rates and a delayed metaphase-anaphase transition, this may have been insufficient to grossly perturb the microtubule-based transport of VACV. Reducing levels of g-CYA did lead to increased speed of VACV undergoing actin-based transport but this did not lead to additional phenotypes in virus release or cell-to-cell spread. The knockdown of g-CYA in HeLa cells causes a compensatory increase in the levels of b-CYA (Figure 2b) , which indicates that comets with greater speeds observed in g-CYA-depleted cells may be caused by a shift in the ratio of CYAs in favor of b-CYA. This observation is in agreement with the previously shown ability of b-CYA to polymerize at faster rates than g-CYA in a reconstituted system (Bergeron, Zhu, Thiem, Friderici, & Rubenstein, 2010) . Virus-associated actin comets were fewer and shorter when levels of b-CYA were reduced. Reductions in actin comet length have been observed previously, either due to mutations in the viral A36 envelope protein, or mutations in cellular N-WASP, which abrogates its ability to bind to actin monomers and reduce its stability during actin polymerization (Horsington et al., 2013; Weisswange et al., 2009 ). As we also observed a significant reduction in the length of actin comets during b-CYA depletion, we hypothesized that altered binding, such as a pref- Co et al., 2007) . The ability of these tagged proteins to bind to actin was examined, and no preference for either CYA isoform was observed by immunoblot. Additionally, phosphorylation of the N-WASP VCA domain has been shown to enhance actin polymerization activity (Cory, Cramer, Blanchoin, & Ridley, 2003) , a post-translational modification that may not be present in bacterially-expressed GST-VCA domains. Nonetheless, our results do not support a specific binding affinity of the WH2 domains of N-WASP for b-CYA as the underlying mechanism that results in a requirement for this isoform in VACV actin-based motility. A recent genome-wide siRNA screen performed during infection of HeLa cells with the bacterial pathogen Listeria, also known to employ actin-based motility for infection (Lambrechts, Gevaert, Cossart, Vandekerckhove, & Van Troys, 2008) , showed that siRNA-mediated knockdown of b-CYA, but not g-CYA, impaired infection (Kuhbacher et al., 2015) . These findings suggest that specificity for actin isoforms for actin nucleation might be at the level of the Arp2/3 complex, rather than with its activator N-WASP. Our most surprising observation was the increase in VACV plaque size during b-CYA knockdown in GBM cells. g-CYA is a known regulator of Rho-associated kinase (ROCK)-mediated cell migration, and the knockdown of g-CYA has been shown to reduce cell migration (Dugina et al., 2009; Shum et al., 2011) . Additionally, we observed a significant increase in g-CYA expression during b-CYA knockdown in GBM cells, which may have had an additive effect, contributing to increased viral plaque size. An enhancement of directional cell migration during b-CYA depletion, as well as a reduction in migration during g-CYA depletion, has also been observed previously (Dugina et al., 2009) . This increase in motility during b-CYA knockdown could also be playing a role in VACV virus-induced cell motility, contributing to an increased plaque size. The VACV protein F11 is also responsible for virusinduced cell migration, through its inhibition of RhoA signalling (Cordeiro, Guerra, Arakawa, Dodding, Esteban, & Way, 2009; Valderrama, Cordeiro, Schleich, Frischknecht, & Way, 2006) . This may explain why we have not observed a corresponding reduction in plaque size during g-CYA knockdown. Interestingly, b-CYA synthesis has also been associated with Rho signalling (Latham, Yu, Tullio, Adelstein, & Singer, 2001 ) and the use of a Rho kinase inhibitor can cause the selective disorganization of b-CYA bundles while leaving g-CYA undisturbed (Dugina et al., 2009) . Clearly there is a strong link between b-CYA and Rho signalling, such that the depletion of b-CYA may have a significant effect on Rho and its downstream signalling targets. The use of ROCK inhibitors under conditions of band g-CYA knockdown may, in future studies, clarify the complex networks involved in this process. It is evident, however, that cell motility is an important determinant of VACV plaque size. This work contributes to the emerging paradigm that not all actin isoforms are equivalent. Studies have shown a specific interaction of b-CYA with viral membrane proteins like the classical swine fever virus E2 protein (He et al., 2014) and the coronavirus M protein (Wang et al., 2009 ). While we have not observed a physical interaction between a specific actin isoform and a VACV protein, our results indicate that b-CYA plays a role in the N-WASP-mediated nucleation of actin beneath VACV particles. The evidence of isoform-specific functions of actin during microbial pathogenesis suggests that the most basic of cellular processes may potentially be therapeutically targetable. Clearly blocking all actin functions would be highly detrimental to a cell. Our results indicate that specific pathogenic mechanisms can be successfully targeted, even for such a fundamental process as actin nucleation, by inhibiting one isoform of a highly conserved group of proteins. Our data was unable to uncover the precise mechanism by which b-CYA actin is required during VACV-induced actin nucleation, but they suggest a link between the activity of a particular nucleator, N-WASP, and an actin isoform, b-CYA. This raises the tantalizing possibility that nucleators may be subject to regulation by isoform availability, which is consistent with the highly localized distributions of b-CYA and actin-CYA at the subcellular level. Cell lines used in this study were HeLa (human cervical cancer cell line, mented with 5% foetal bovine serum (FBS) and 292 mg/ml L-glutamine, 100 units/ml penicillin and 100 mg/ml streptomycin at 378C in 5% CO2 (DMEM-FBSG). hCMEC/D3 cells were cultured in supplemented EBM-2 medium (Lonza), as described by Latham et al. (Latham et al. 2013) . Viruses used in this study were the VACV strain Western Reserve (VACV-WR, ATCC VR-1354), the WR strain where the gene A36 contains two point mutations (VACV-A36 YdF , (Scaplehorn et al., 2002) , and WR VACV-Lifeact-GFP, which is a VACV-WR strain carrying a Lifeact-GFP transgene expressed through a synthetic early/late VACV promoter. Lifeact is a 17-amino acid peptide that is used to visualize actin (Riedl, Crevenna, Kessenbrock, Yu, Neukirchen, Bista, . . . Wedlich-Soldner, 2008) , and when bound to GFP enables visualization of the F-actin cytoskeleton in live cells. Lifeact-GFP was cloned and inserted between VACV genes C10L and C11R by homologous recombination as previously described (Marzook et al., 2014) . Mouse antibodies against b-CYA and g-CYA were a gift (Dugina et al., 2009 OptiMEM (Invitrogen) for six hours, followed by replacement with the respective complete media. Knockdown was allowed to proceed for 72 hr before prior to analysis by immunoblot, immunofluorescence or plaque assay. Cells treated with siRNA were lysed after 72 hr in sodium dodecyl sul- Amersham Biosciences) and probed with primary antibodies diluted in PBST-milk (5% [wt/vol] skim milk in PBS with 0.1% Tween 20), followed by washing three times in PBST-milk. The membrane was then probed with secondary antibodies conjugated with horseradish peroxidase. Immunoreactive protein bands were visualized with ECL Western blotting reagent (GE Healthcare) and developed using a CP1000 photographic film developer. Blots were scanned using the GS-900 calibrated densitometer (Bio-Rad), densities were measured using FIJI image processing software, and statistical analysis was done using GraphPad PRISM. hCMEC/D3, HeLa or GBM cells were grown on glass coverslips to about 80% confluency (72 hr post-siRNA treatment) and infected with VACV-WR at an MOI > 5. Cells were fixed 8 hpi, the optimum time to observe VACVassociated actin comets (Cudmore et al., 1995) , in 3% paraformalde- Z-stack images were collected with the Zeiss LSM 510 confocal microscope at 633 magnification with 1.4 NA at room temperature. The resulting images were processed with Photoshop CS3 (Adobe; to separate RGB color channels and crop images), and analysed using FIJI (for measuring actin comet length and plaque diameter) and GraphPad PRISM (for data visualization and statistical measurements). Cells infected with VACV-WR Lifeact-GFP were imaged using the Nikon Eclipse Ti-E inverted microscope system at 403 magnification in a chamber maintained at 378C in a 5% CO 2 -enriched atmosphere. Images were captured every 4 s over a period of 5 min using NIS-Elements AR (v4.51.00) image capture software. Maximal intensity projections for 1 min intervals over the 5 min time course were created, and lengths of actin comets in these projections were measured using FIJI (ver. 2.0.0-rc-43). Speed was calculated as length of comets over the time interval of that projection (1 min in this case). A plasmid expressing GST-VCA was created by PCR from a plasmid containing rat N-WASP O08816 (forward primer containing a Not restriction site: 5 0 GGCGGCCGCGACCATCAAGTTCCAGC 3 0 , and reverse primer containing an Eco restriction site: 5 0 GAATTCT-CAGTCTTCCCACTCATC 3 0 ), creating a 333 kb oligonucleotide. This was ligated into a pMW T7 vector containing GST, as described previously by (Newsome et al., 2006 ; using the NotI -EcoRI restriction sites). The GST-VCA-RA/RA plasmid was created using the plasmid previously described, where a 333 kb VCA RA/RA oligonucleotide (containing mutations corresponding to positions R410A and R438A in the full-length rat N-WASP) was generated synthetically (Integrated DNA Technologies), and ligated into the backbone using the same restriction sites (NotI -EcoRI). Plasmids expressing either GST-VCA or GST-VCA-RA/RA were transformed into bacterial BL21 competent E. coli (NEB) and grown at 378C until they reached an OD of around 0.5. Bacterial cells were lysed in lysis buffer [1% Triton-X (v/v) and 200 uM phenylmethylsulfonyl fluoride (PMSF) in PBS] by sonication, and passed over glutathione-containing Sepharose beads (GE Healthcare), primed in lysis buffer not containing PMSF. Three rounds of washing in lysis buffer enriched for GST-bound expressed protein from the bacterial cells. HeLa cell lysates (prepared with the same lysis buffer) were then passed over the beads, followed by washing three times. Beads were then boiled in SDS reducing sample buffer (described above) and separated by SDS-PAGE, followed by immunoblotting. Following siRNA treatment for 72 hr, GBM cells that had reached confluency were infected by VACV-WR at an MOI of 0.1. 1 hpi, cells were washed and over-laid with modified Eagle medium (MEM; Invitrogen) supplemented with 5% FBS, 292 lg/ml L-glutamine, 100 units/ml penicillin, 100 lg/ml streptomycin, and 1.5% carboxymethyl cellulose. Plaques were allowed to form for 3 days before being fixed with 1% crystal violet in methanol. Cell culture plates were scanned using the GS-900 densitometer (Bio-Rad) and plaque diameters from two experiment replicates were measured using FIJI image processing software. Following siRNA treatment for 72 hr, cells HeLa cells were infected by the designated virus (either VACV-WR or VACV-A36 YdF ), at an MOI of 0.1 for 1 hr. Cells were then washed twice and overlaid with DMEM-FBSG. Supernatants were collected at 16 hpi and plaque assays using 10-fold serial dilutions of the supernatant were conducted on BSC-1 cells, as described above. Plaques were enumerated from three experimental replicates and statistical analysis was carried out with GraphPad PRISM software. The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility A role for the small GTPase Rac1 in vaccinia actin-based motility Planning your every move: the role of beta-actin and its posttranscriptional regulation in cell motility Ion-dependent polymerization differences between mammalian beta-and gamma-nonmuscle actin isoforms Pathological situations characterized by altered actin isoform expression Mechanism of actin network attachment to moving membranes: barbed end capture by N-WASP WH2 domains F11-mediated inhibition of RhoA signalling enhances the spread of vaccinia virus in vitro and in vivo in an intranasal mouse model of infection Phosphorylation of the WASP-VCA domain increases its affinity for the Arp2/3 complex and enhances actin polymerization by WASP Actin-based motility of vaccinia virus Repulsion of superinfecting virions: A mechanism for rapid virus spread Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity Tyrosine phosphorylation is required for actinbased motility of vaccinia but not Listeria or Shigella Actin-based motility of vaccinia virus mimics receptor tyrosine kinase signalling Actin-based motility of intracellular pathogens Effects of crosslinked profilin:beta/gamma-actin on the dynamics of the microfilament system in cultured cells Betaactin interacts with the E2 protein and is involved in the early replication of classical swine fever virus Vaccinia virus utilizes microtubules for movement to the cell surface A36-dependent actin filament nucleation promotes release of vaccinia virus Clathrin potentiates vaccinia-induced actin polymerization to facilitate viral spread Technical knockout: understanding poxvirus pathogenesis by selectively deleting viral immunomodulatory genes Downregulation of beta-actin and its regulatory gene HuR affect cell migration of human corneal fibroblasts Mechanisms of spatial segregation of actin isoforms Genome-Wide siRNA Screen Identifies Complementary Signaling Pathways Involved in Listeria Infection and Reveals Different Actin Nucleation Mechanisms during Listeria Cell Invasion and Actin Comet Tail Formation Listeria comet tails: The actin-based motility machinery at work Cooperation between beta-and gammacytoplasmic actins in the mechanical regulation of endothelial microparticle formation A Rho-dependent signaling pathway operating through myosin localizes beta-actin mRNA in fibroblasts Loss of gamma-cytoplasmic actin triggers myofibroblast transition of human epithelial cells Loss of cytoskeletal transport during egress critically attenuates ectromelia virus infection in vivo Methodology for the efficient generation of fluorescently tagged vaccinia virus proteins Poxvirus tropism A complex of N-WASP and WIP integrates signalling cascades that lead to actin polymerization Viruses that ride on the coattails of actin nucleation SRC mediates a switch from microtubule-to actin-based motility of vaccinia virus Abl collaborates with Src family kinases to stimulate actin-based motility of vaccinia virus Actin-based motility drives baculovirus transit to the nucleus and cell surface Vaccinia virus gene A36R encodes a M(r) 43-50 K protein on the surface of extracellular enveloped virus gamma-Actin plays a key role in endothelial cell motility and neovessel maintenance The effect of cytochalasin D and monensin on enveloped vaccinia virus release Partial depletion of gamma-actin suppresses microtubule dynamics Viral interactions with the cytoskeleton: a hitchhiker's guide to the cell Disabling poxvirus pathogenesis by inhibition of Abl-family tyrosine kinases Lifeact: A versatile marker to visualize F-actin Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus Replacing the SCR domains of vaccinia virus protein B5R with EGFP causes a reduction in plaque size and actin tail formation but enveloped virions are still transported to the cell surface The functional importance of multiple actin isoforms Grb2 and Nck act cooperatively to promote actin-based motility of vaccinia virus Actin and alphaactinin dynamics in the adhesion and motility of EPEC and EHEC on host cells Gamma-Actin regulates cell migration and modulates the ROCK signaling pathway The exit of vaccinia virus from infected cells The formation and function of extracellular enveloped vaccinia virus Immune response to poxvirus infections in various animals N-WASP deficiency reveals distinct pathways for cell surface projections and microbial actin-based motility The WASP-WAVE protein network: Connecting the membrane to the cytoskeleton Actin filament nucleation by the bacterial pathogen, Listeria monocytogenes Vaccinia virus-induced cell motility requires F11L-mediated inhibition of RhoA signaling Mammalian cytoplasmic actins are the products of at least two genes and differ in primary structure in at least 25 identified positions from skeletal muscle actins. Proceedings of the At least six different actins are expressed in a higher mammal: An analysis based on the amino acid sequence of the amino-terminal tryptic peptide Interaction of the coronavirus infectious bronchitis virus membrane protein with beta-actin and its implication in virion assembly and budding Ch 16 Vaccinia Virus and Poxvirology Vaccinia virus intracellular movement is associated with microtubules and independent of actin tails The rate of N-WASP exchange limits the extent of ARP2/3-complex-dependent actin-based motility The hCMEC/D3 cell line as a model of the human blood brain barrier Arp2/3-mediated actin-based motility: A tail of pathogen abuse Poxvirus host range genes Deletion of the vaccinia virus B5R gene encoding a 42-kilodalton membrane glycoprotein inhibits extracellular virus envelope formation and dissemination The A34R glycoprotein gene is required for induction of specialized actin-containing microvilli and efficient cell-to-cell transmission of vaccinia virus Role for the vaccinia virus A36R outer envelope protein in the formation of virus-tipped actincontaining microvilli and cell-to-cell virus spread The authors would like to thank Prof. C. Chaponnier for the anti-b-