key: cord-0815266-wjo8i239 authors: Mori, Hitoshi; Obinata, Hirofumi; Murakami, Wakana; Tatsuya, Kodama; Sasaki, Hisashi; Miyake, Yu; Taniguchi, Yasuaki; Ota, Shinichiro; Yamaga, Mitsuki; Suyama, Yohsuke; Tamura, Kaku title: Comparison of COVID-19 Disease between Young and Elderly patients: Hidden Viral Shedding of COVID-19 date: 2020-09-06 journal: J Infect Chemother DOI: 10.1016/j.jiac.2020.09.003 sha: 1595204689ded737eebbf678f4013e6672f98cf7 doc_id: 815266 cord_uid: wjo8i239 Objectives The symptoms of Coronavirus disease 2019 (COVID-19) vary among patients. The aim of this study was to investigate the clinical manifestation and disease duration in young versus elderly patients. Methods We retrospectively analyzed 187 patients (87 elderly and 100 young patients) with confirmed COVID-19. The clinical characteristics and chest computed tomography (CT) extent as defined by a score were compared between the two groups. Results The numbers of asymptomatic cases and severe cases were significantly higher in the elderly group (elderly group vs. young group; asymptomatic cases, 31 [35.6%] vs. 10 [10%], p<0.0001; severe cases, 25 [28.7%] vs. 8 [8.0%], p=0.0002). The proportion of asymptomatic patients and severe patients increased across the 10-year age groups. There was no significant difference in the total CT score and number of abnormal cases. A significant positive correlation between the disease duration and patient age was observed in asymptomatic patients (ρ=0.4570, 95% CI 0.1198-0.6491, p=0.0034). Conclusions Although the extent of lung involvement did not have a significant difference between the young and elderly patients, elderly patients were more likely to have severe clinical manifestations. Elderly patients were also more likely to be asymptomatic and a source of COVID-19 viral shedding. Coronavirus disease 2019 , which leads to severe acute respiratory distress syndrome (ARDS), was first reported in Wuhan, Hubei Province, China in December 2019 [1] [2] [3] . It spread rapidly to other countries, and the number of affected patients is increasing. The World Health Organization declared a pandemic on March 11, 2020. There were 3,767,744 confirmed COVID-19 cases and 259,593 related deaths in the world by May 8, 2020 4 . The symptoms of COVID-19 varied among the patients. Patients with minor symptoms presented with a headache, fever, fatigue, and/or cough, and those symptoms improved without any specific treatment. Severe cases presented with severe dyspnea or hypoxia and needed intensive care. An older age is reported to be associated with the disease severity and a high mortality 2 . On the other hand, some COVID-19 patients had no symptoms, even though they had radiological abnormalities 5 . These asymptomatic carriers could result in person-to-person transmission and are considered a source of the COVID-19 infection [6] [7] [8] [9] . Although Liu et al. reported the clinical features of in elderly patients 10 , the manifestation of asymptomatic cases or the disease duration are not well understood. The aim of this study was to investigate the clinical manifestation and disease duration of elderly patients, including both symptomatic and asymptomatic cases. J o u r n a l P r e -p r o o f We retrospectively analyzed 192 patients with confirmed COVID-19 who were admitted to our hospital from February 11 to April 30, 2020. All patients were diagnosed with COVID-19 infections by a viral reverse transcription polymerase chain reaction (RT-PCR). The RT-PCR using a pharyngeal swab was performed when the patients had clinical symptoms or close contact with a known COVID-19 patient. In the symptomatic patients, an initial RT-PCR from a pharyngeal source was performed 2 days after the resolution of the clinical symptoms. In the asymptomatic patients, the RT-PCR was performed 2-5 days after admission. The RT-PCR was repeatedly performed every 1-2 days. Discharge criteria included two consecutive negative RT-PCR results. The definition of asymptomatic cases was as follows: COVID-19 was confirmed by the RT-PCR, but carriers did not have any clinical symptoms (some despite radiological abnormalities). Severe cases were defined as those with hypoxia requiring oxygen therapy (due to the pneumonia). Antiviral therapy or corticosteroids were administered for severe cases at the discretion of the clinical attending physician. Our hospital treated all COVID-19 patients in the general wards; therefore, no J o u r n a l P r e -p r o o f cases were treated in the intensive care unit. Patients younger than 18 years old were excluded from this analysis. A total of 187 patients were enrolled in the study and divided into an elderly group (≧60 years old) and young group (<60 years old). For each COVID patient, the laboratory results and chest computed tomography (CT) studies were evaluated within the 48 hours after the first admission. Our clinical data were collected retrospectively from the institutional medical records by two physicians (H.M. and H.O.). The following data were extracted: clinical information, clinical symptoms, and laboratory findings. The disease duration was defined as that from the symptom onset to the first RT-PCR negative result in symptomatic patients and from a RT-PCR positive test to the first RT-PCR negative test in asymptomatic patients. The CT findings were reviewed by two radiologists with 5 and 11 years of experience (W.M. and Y.S.) blinded to the clinical information, and the final decision was reached by consensus. For all the patients, axial CT images were evaluated semi-quantitatively using a scoring system for the J o u r n a l P r e -p r o o f 8 COVID-19 findings based on the previous studies 11, 12 . Each lung was divided into six zones without regard to the anatomical lobe; for the cranial-caudal dimension, three zones were defined as upper (above the carina), middle (below the carina and above the inferior pulmonary vein), and lower (below the inferior pulmonary vein). For the medial-lateral dimension, each outer third of the axial slice was defined as peripheral, and the middle third area was defined as central. Therefore, a total of 12 lung zones were presented in each patient. Each zone was graded according to a visual assessment of the involvement as follows: 0 (0%), 1 (1-5%), 2 (6-25%), 3 (25-50%), 4 (51-75%), and 5 (75-100%). The final score was summed over all 12 zones and ranged from 0 (no involvement) to 60 (maximum involvement). The clinical characteristics and clinical manifestations were compared between the two groups. The correlation between the patient age and disease duration was also analyzed. Statistical analyses were performed using JMP® Pro software, version 11.2 (SAS Institute). A Shapiro-Wilk test was performed to assess the data distribution. The continuous variables were represented by median values with interquartile ranges and compared using a Mann-Whitney test for J o u r n a l P r e -p r o o f non-parametric data. The categorical data were compared using a chi-square test. A Spearman's correlation test was performed to assess the relationship between the age and number of admission days or disease duration. The Cochran-Armitage test was used for a trend analysis. A value of P < 0.05 indicated statistical significance. A total of 187 patients were enrolled in our study, 87 elderly patients and 100 young patients. Table 1 shows the clinical characteristics of the two groups. The median age of the elderly group was 72 years (IQR; 67-76 years), and the median age of the young group was 41 years (IQR; 35-48 years). The patents in the elderly group were more often female and more likely to have hypertension, diabetes, and cardiovascular disease. Although the number of asymptomatic cases was significantly higher in the elderly group (elderly group 31 (35.6%) than young group 10 (10%), p<0.0001), the number of severe cases was significantly higher in the elderly group (elderly group 25 (28.7%) than young group 8 (8.0%), p=0.0002). The younger group patients had a fever, fatigue, headache, cough, myalgia, and diarrhea J o u r n a l P r e -p r o o f with statistical significance. The most common symptom among the young group was a fever (elderly group 41 [47.1%] vs. young group 77 [77%], p<0.0001). A trend analysis was performed to assess the proportion of clinical characteristics. One 18 year-old patient was included in the 20-year group. The proportions of asymptomatic patients and severe patients both increased across the age groups ( Figure 1A and 1B) . On the other hand, the proportion of patients with a fever decreased across the age groups ( Figure 1C ) A total of 185 patients underwent chest CT studies. Two patients with severe emphysema, one with severe interstitial pneumonia, one with non-tuberculous mycobacteria, and one with excessive motion artifact were excluded from the CT analysis. The remaining 180 CT studies were reviewed. Table 3 shows the CT findings of the asymptomatic patients separated into the two groups. There was also no significant difference in the total CT score Table 4 shows the disease duration and days of admission in the two groups. Two symptomatic elderly patients, whose disease onset was not recorded, and ten symptomatic young patients, who were discharged before the RT-PCR was confirmed negative, were excluded. One asymptomatic elderly patient, in whom the day of the positive RT-PCR result was not recorded, and one asymptomatic elderly patient, who underwent the RT-PCR on day 8 after admission, were also excluded. The days of admission in the symptomatic patients among the elderly group were significantly longer than that in the young group. The disease duration and days of admission in the asymptomatic patients in the elderly group were also significantly longer than that in the young group. Spearman's for admission days, ρ=0.5583, 95% CI 0.2104-0.6998, p=0.0002). To the best of our knowledge, this is the first report to compare the clinical manifestation and duration of the disease between elderly and young COVID-19 patients, which includes both symptomatic cases and asymptomatic cases. The main findings of this study were: (1) while there were many severe patients in the elderly group, elderly patients were more often asymptomatic and afebrile; (2) there was no significant difference in the total CT score and number of abnormal cases between the elderly and young groups; and (3) the duration of the disease had a significant positive correlation with the patient age in the asymptomatic group. Although there were no significant differences in the CT score, the elderly patients had a higher incidence of severe and asymptomatic cases. Such cases with CT findings and a lack of clinical J o u r n a l P r e -p r o o f show any statistical difference between the two groups. This may indicate that lung damage from COVID-19 was similar between the two groups, despite the varying clinical manifestations. Differences in the elderly group's lung anatomy, including muscle atrophy, may account for the higher severity among the elderly, with reduced the airway clearance 13 , lung reserve 14 , and defense barrier function 15 . A prior study associated hypertension with the COVID-19 disease severity 16 . Higher blood pressure may be associated with the clinical manifestations and outcomes. A lower lymphocyte ratio in the elderly group may also be associated with the greater disease severity among the elderly group. Another study reported a significant positive correlation between the age and peak viral load 18 . Our study showed that the duration of the disease had a significant positive correlation with the patient age in the asymptomatic group. The viral load in the asymptomatic patients was reported to be similar to that in the symptomatic patients, which suggested the transmission potential of asymptomatic patients 8 . Elderly patients are more likely to be asymptomatic with a higher viral load. Special consideration should be given to this population for the purpose of infection control during this pandemic. Our study had a few sources of potential selection bias. This study was a single center retrospective study with a relatively small sample size. Although our study included 33 severe cases, including four intubated patients, COVID-19 patients were triaged before admission, and severe cases were admitted preferentially to a different hospital. In addition, CT images were obtained and analyzed at an early stage of COVID-19. Presumably, later CT images would show the disease progression not captured in this study. Finally, we were unable to address the relationship between the mortality and patient age. Due to the limitation in the number of RT-PCR tests during the initial phase of the COVID-19 pandemic, the timing of the RT-PCR test for evaluating negative results was irregular among the patients. Table 3 . CT findings of the two groups (asymptomatic patients) J o u r n a l P r e -p r o o f Duration of disease, days 10 (7.5-15.5) 7.5 (7.0-10.0) 0.0496* Clinical features of patients infected with 2019 novel coronavirus in Wuhan Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus-Infected Pneumonia in Wuhan, China Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study WHO Coronavirus disease (COVID-2019) Dashboard The clinical characteristics of COVID-19: a retrospective analysis of 104 patients from the outbreak on board the Diamond Princess cruise ship in Japan Presumed Asymptomatic Carrier Transmission of COVID-19 Transmission of 2019-nCoV Infection from an Asymptomatic Contact in Germany SARS-CoV-2 Viral Load in Upper Respiratory Specimens of Infected Patients Clinical characteristics of 24 asymptomatic infections with COVID-19 screened among close contacts in Nanjing Clinical features of COVID-19 in elderly patients: A comparison with young and middle-aged patients Time Course of Lung Changes On Chest CT During Recovery From 2019 Novel Coronavirus (COVID-19) Pneumonia. 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