key: cord-0825534-hxdzelz9 authors: Montana, Marc; Montero, Vincent; Khoumeri, Omar; Vanelle, Patrice title: Quinoxaline Derivatives as Antiviral Agents: A Systematic Review date: 2020-06-16 journal: Molecules DOI: 10.3390/molecules25122784 sha: d972da0798ff9f53345ef49e788eb6042949f977 doc_id: 825534 cord_uid: hxdzelz9 Background: In recent decades, several viruses have jumped from animals to humans, triggering sizable outbreaks. The current unprecedent outbreak SARS-COV-2 is prompting a search for new cost-effective therapies to combat this deadly pathogen. Suitably functionalized polysubstituted quinoxalines show very interesting biological properties (antiviral, anticancer, and antileishmanial), ensuring them a bright future in medicinal chemistry. Objectives: Focusing on the promising development of new quinoxaline derivatives as antiviral drugs, this review forms part of our program on the anti-infectious activity of quinoxaline derivatives. Methods: Study compiles and discusses recently published studies concerning the therapeutic potential of the antiviral activity of quinoxaline derivatives, covering the literature between 2010 and 2020. Results: A final total of 20 studies included in this review. Conclusions: This review points to a growing interest in the development of compounds bearing a quinoxaline moiety for antiviral treatment. This promising moiety with different molecular targets warrants further investigation, which may well yield even more encouraging results regarding this scaffold. Humans have a long history of viral infections. For some viral diseases, vaccines and antiviral drugs have made it possible to prevent infections or have helped those infected to recover. Recent antiviral drug development has led to the discovery of effective new treatments to control Human Immunodeficiency Virus (HIV) and Hepatitis C virus (HCV) infections [1] . However, several viruses have jumped from animals to humans triggering sizable outbreaks. One example is the 2014-2016 outbreak of Ebola in West Africa, which resulted in over 28,000 infected patients and was responsible for over 11,000 deaths [2] , making it the most lethal member of the Ebola family. The SARS-COV-2 outbreak worldwide continues to pose a serious threat to public health, with no reliable treatment yet available. In addition, the last decade has seen the development of only a few novel antivirals as remdesivir and favipiravir, initially used respectively as Ebola and influenzae treatment and proposed for repurposing in the SARS-CoV-2 outbreak, or sofosbuvir and daclatasvir, which have dramatically changed the prognosis in HCV infection [3, 4] . However, this unprecedented SARS-CoV-2 crisis underlines the urgency of developing new cost-effective therapies to combat the deadly pathogen. Nitrogen-containing heterocycles are promising compounds for the development of new drugs or novel potential lead molecules [5, [6] [7] [8] . The quinoxaline scaffold, a bioisoster of quinoline and naphthalene, is one of the heterocycles currently attracting attention. Quinoxaline, formed by the fusion of benzene and pyrazine rings, is a white crystalline powder whose melting point is 29-30 °C and whose molecular formula is C8H6N2 [9, 10] . Its synthesis has been intensively studied in the past. The classic method of quinoxaline preparation is to condense ophenylenediamine with a dicarbonyl compound. This procedure requires high temperatures, a strong acid catalyst, and long reaction times [11, 12] . Other strategies described for the synthesis of quinoxaline derivatives involve condensation of 1,2-diamines with α-diketones [13] , 1,4-addition of 1,2-diamines to diazenylbutenes [14] , cyclization-oxidation of phenacyl bromides [15] , and oxidative coupling of epoxides with ene-1,2-diamines [16] . There are also several green synthetic methods, like using recyclable catalysts [17] , one-pot synthesis [18] , microwave-assisted synthesis [19, 20] , and reactions in aqueous medium [21] . Suitably functionalized polysubstituted quinoxalines show very interesting biological properties (antiviral [22] , anticancer [23] , and antileishmanial [24] ), ensuring them a bright future in medicinal chemistry [11, 25] . Many drug candidates bearing quinoxaline core structures have been identified, such as S-2720 ( Figure 1 ), found to be a very potent inhibitor of HIV-1 reverse transcriptase [26] . This review investigates the new quinoxaline derivatives that are showing promise as antiviral drugs as part of our program focused on the anti-infectious activity of quinoxaline derivatives. It compiles and discusses recently published studies concerning the therapeutic potential of the antiviral activity of quinoxaline derivatives, covering the literature between 2010 and 2020. The search method employed in this systematic review was to select studies that evaluated the biological activity and mechanism of action of quinoxaline derivatives. Three different databases were used to conduct a comprehensive survey: MEDLINE/PubMed (National Library of Medicine-www.ncbi.nlm.nih.gov/pubmed), Web of Science (Thomson Reuters Scientific-www.webofknowledge.com/), and Science Direct (Elsevier www.sciencedirect.com). The search terms "quinoxaline" and "antiviral" were chosen so as to detect everything published about quinoxaline before applying exclusion criteria. Searches were conducted using the limit dates of 1 January 2010 and 1 May 2020. The review was performed in three main stages by three independent reviewers. In the first stage, articles' titles and abstracts were assessed according to the eligibility criteria (Table 1 ). In the second stage, duplicated articles were deleted. Finally, the authors read each selected full text and This review investigates the new quinoxaline derivatives that are showing promise as antiviral drugs as part of our program focused on the anti-infectious activity of quinoxaline derivatives. It compiles and discusses recently published studies concerning the therapeutic potential of the antiviral activity of quinoxaline derivatives, covering the literature between 2010 and 2020. The search method employed in this systematic review was to select studies that evaluated the biological activity and mechanism of action of quinoxaline derivatives. Articles that evaluate the biological activity of quinoxaline derivatives Articles that concern non-human or animal viruses The following information was extracted from all the selected studies: type of study, biological matrix used, compound structure and nomenclature, and main conclusions. The database search identified 216 records. After the first evaluation phase (title/abstract), 175 records were excluded. Eight repeated files were discarded, leaving 33 articles. No other paper was added from the reference lists of the identified studies (which had not been found in the initial search). A second phase was therefore conducted with a total of 33 articles. After the full-text reading, 13 articles were excluded and one was included, leaving a final total of 21 studies included in this review. This process is illustrated by a flow diagram in Figure 2 . After critical reading, articles were divided into categories according to the activity assessed in each study (Table 2) . A manuscript could be assigned to more than one category if a single study evaluated more than one type of antiviral activity. DNA viruses have DNA genomes that are replicated by either host or virally encoded DNA polymerases. DNA viruses with a large genome, particularly the families of Herpesviridae and Poxviridae, encode a number of proteins that counter host defenses. [48] Double-stranded DNA viruses can be subdivided into three groups: (1) those with a small size DNA genome (<10 kb), such as polyomaviruses and papillomaviruses; (2) those with a medium-sized DNA genome (ca., 35 kb), such as adenoviruses; and (3) those with a large DNA genome (ca., 130-250 kb), such as herpesviruses and poxviruses [49] . The Herpesviridae are a ubiquitous worldwide family responsible for viral infections. Among its members frequently encountered are, herpes simplex viruses (HSV-1 and HSV-2), human cytomegalovirus (HCMV), and Epstein Barr virus (EBV) [28, 50, 51] . Usually, these infections remain latent and patients are often asymptomatic, particularly immunocompetent populations. However, in immunocompromised patients especially (e.g., AIDS, cancer, etc.), there can be clinical symptoms such as meningitidis or pneumoniae leading to death. Treatments against HSV or HCMV like ganciclovir, valganciclovir, foscarnet, or cidofovir, are currently available but they are limited by toxicity and/or poor oral bioavailability [28, 52, 53] . In addition, drug resistance is emerging. Hence there is a need to identify improved agents that circumvent one or more of these problems. In 2012, a series of new [1, 2, 4] triazolo[4,3-a]quinoxaline derivatives and their pyrimido-quinoxaline isosters were synthesized and evaluated as potential antiviral agents. Twenty-two novel compounds were obtained. Among them, 1-(4-chloro-8-methyl [1, 2, 4] triazolo [4,3a] quinoxaline-1-yl)-3-phenyl thiourea 1 showed the highest antiviral activity in a plaque-reduction assay against Herpes simplex virus grown on Vero African monkey kidney cells, reducing the number of plaques by 25% at 20 µg/mL ( Figure 3 ). Nine other compounds reduced the number of plaques by less than 25% at 80 µg/mL, leading the authors to the conclusion that the thiourea moiety may be responsible for antiviral activity and highlighting the importance of the moieties selected in developing antiviral activity [29] . However, antiviral activity remained disappointing compared to positive control aphidicolin, which reduced the number of plaques by 100% at 5 µg/mL, even though compound 1 showed lower cytotoxicity. Figure 3 . Chemical structure of 1-(4-chloro-8-methyl [1, 2, 4] triazolo [4,3a] quinoxaline-1-yl)-3-phenyl thiourea 1. The synthesis of four new aldehydo-sugar-N-(3-phenylquinoxalin-2-yl)hydrazones 2a-d and their acyclic C-nucleoside analogues, 1-(4-phenyl- [1, 2, 4] triazolo[4,3-a]quinoxalin-1-yl)alditols 3a-d ( Figure 4 ) indicated that these compounds exhibited very weak antiviral activity against HSV-1 in a plaque reduction infectivity assay in comparison to aphidicolin taken as reference [30] . Recently, nine novel quinoxaline derivatives were synthetized via different nucleophilic reactions using ethyl (6,7-dimethyl-2-oxo-3,4-dihydroquinoxalin-3-yl)acetate 4 and ethyl (6-methyl-2-oxo-3,4-dihydroquinoxalin-3-yl)acetate 5, 3-methylquinoxalin-2(1H)-one, and 1,4dihydroquinoxaline-2,3-dione as precursors [31] (Table 3) . When their antiviral activity against HCMV was compared to the standard drug ganciclovir (EC50 = 0.059 µM), two derivatives demonstrated higher activity, each with EC50 < 0.05 µM. Notably, the toxicity of 4 and 8 (CC50 = 108.47 and >150 µM, respectively) was comparable to the reference drug (CC50 >150 µM) and compound 6 showed the poorest safety profile with CC50 = 2.34 µM. The synthesis of four new aldehydo-sugar-N-(3-phenylquinoxalin-2-yl)hydrazones 2a-d and their acyclic C-nucleoside analogues, 1-(4-phenyl- [1, 2, 4] triazolo[4,3-a]quinoxalin-1-yl)alditols 3a-d ( Figure 4 ) indicated that these compounds exhibited very weak antiviral activity against HSV-1 in a plaque reduction infectivity assay in comparison to aphidicolin taken as reference [30] . The synthesis of four new aldehydo-sugar-N-(3-phenylquinoxalin-2-yl)hydrazones 2a-d and their acyclic C-nucleoside analogues, 1-(4-phenyl- [1, 2, 4] triazolo[4,3-a]quinoxalin-1-yl)alditols 3a-d ( Figure 4 ) indicated that these compounds exhibited very weak antiviral activity against HSV-1 in a plaque reduction infectivity assay in comparison to aphidicolin taken as reference [30] . Recently, nine novel quinoxaline derivatives were synthetized via different nucleophilic reactions using ethyl (6,7-dimethyl-2-oxo-3,4-dihydroquinoxalin-3-yl)acetate 4 and ethyl (6-methyl-2-oxo-3,4-dihydroquinoxalin-3-yl)acetate 5, 3-methylquinoxalin-2(1H)-one, and 1,4dihydroquinoxaline-2,3-dione as precursors [31] (Table 3) . When their antiviral activity against HCMV was compared to the standard drug ganciclovir (EC50 = 0.059 µM), two derivatives demonstrated higher activity, each with EC50 < 0.05 µM. Notably, the toxicity of 4 and 8 (CC50 = 108.47 and >150 µM, respectively) was comparable to the reference drug (CC50 >150 µM) and compound 6 showed the poorest safety profile with CC50 = 2.34 µM. Recently, nine novel quinoxaline derivatives were synthetized via different nucleophilic reactions using ethyl (6,7-dimethyl-2-oxo-3,4-dihydroquinoxalin-3-yl)acetate 4 and ethyl (6-methyl-2-oxo-3,4dihydroquinoxalin-3-yl)acetate 5, 3-methylquinoxalin-2(1H)-one, and 1,4-dihydroquinoxaline-2,3-dione as precursors [31] (Table 3) . When their antiviral activity against HCMV was compared to the standard drug ganciclovir (EC 50 = 0.059 µM), two derivatives demonstrated higher activity, each with EC 50 < 0.05 µM. Notably, the toxicity of 4 and 8 (CC 50 = 108.47 and >150 µM, respectively) was comparable to the reference drug (CC 50 >150 µM) and compound 6 showed the poorest safety profile with CC 50 = 2.34 µM. Four other compounds showed promising antiviral activity. Antiviral activity was observed to depend on varying chemical characteristics, like the presence of a dimethylquinoxalinyl methylene nucleus as a common structural feature and the presence of a lipophilic ester function ( Figure 5 ). Four other compounds showed promising antiviral activity. Antiviral activity was observed to depend on varying chemical characteristics, like the presence of a dimethylquinoxalinyl methylene nucleus as a common structural feature and the presence of a lipophilic ester function ( Figure 5 ). The presence of a second CH 3 favors antiviral actvity A lipophilic side chain enhances the activity The methylene bridge is necessary for activity In addition, several quinoxaline derivatives are of current interest as anti HCMV agents [28] . Some 2-aryl-2-hydroxy ethylamine substituted 1H,7H-pyrido[1,2,3-de]quinoxaline-6-carboxamides were synthetized and tested ( Figure 6 ). Four other compounds showed promising antiviral activity. Antiviral activity was observed to depend on varying chemical characteristics, like the presence of a dimethylquinoxalinyl methylene nucleus as a common structural feature and the presence of a lipophilic ester function ( Figure 5 ). The presence of a second CH 3 favors antiviral actvity A lipophilic side chain enhances the activity The methylene bridge is necessary for activity In addition, several quinoxaline derivatives are of current interest as anti HCMV agents [28] . Some 2-aryl-2-hydroxy ethylamine substituted 1H,7H-pyrido[1,2,3-de]quinoxaline-6-carboxamides were synthetized and tested ( Figure 6 ). Four other compounds showed promising antiviral activity. Antiviral activity was observed to depend on varying chemical characteristics, like the presence of a dimethylquinoxalinyl methylene nucleus as a common structural feature and the presence of a lipophilic ester function ( Figure 5 ). The presence of a second CH 3 favors antiviral actvity A lipophilic side chain enhances the activity The methylene bridge is necessary for activity In addition, several quinoxaline derivatives are of current interest as anti HCMV agents [28] . Some 2-aryl-2-hydroxy ethylamine substituted 1H,7H-pyrido[1,2,3-de]quinoxaline-6-carboxamides were synthetized and tested ( Figure 6 ). Four other compounds showed promising antiviral activity. Antiviral activity was observed to depend on varying chemical characteristics, like the presence of a dimethylquinoxalinyl methylene nucleus as a common structural feature and the presence of a lipophilic ester function ( Figure 5 ). The presence of a second CH 3 favors antiviral actvity A lipophilic side chain enhances the activity The methylene bridge is necessary for activity In addition, several quinoxaline derivatives are of current interest as anti HCMV agents [28] . Some 2-aryl-2-hydroxy ethylamine substituted 1H,7H-pyrido[1,2,3-de]quinoxaline-6-carboxamides were synthetized and tested ( Figure 6 ). Four other compounds showed promising antiviral activity. Antiviral activity was observed to depend on varying chemical characteristics, like the presence of a dimethylquinoxalinyl methylene nucleus as a common structural feature and the presence of a lipophilic ester function ( Figure 5 ). The presence of a second CH 3 favors antiviral actvity A lipophilic side chain enhances the activity The methylene bridge is necessary for activity In addition, several quinoxaline derivatives are of current interest as anti HCMV agents [28] . Some 2-aryl-2-hydroxy ethylamine substituted 1H,7H-pyrido[1,2,3-de]quinoxaline-6-carboxamides were synthetized and tested ( Figure 6 ). Four other compounds showed promising antiviral activity. Antiviral activity was observed to depend on varying chemical characteristics, like the presence of a dimethylquinoxalinyl methylene nucleus as a common structural feature and the presence of a lipophilic ester function ( Figure 5 ). The presence of a second CH 3 favors antiviral actvity A lipophilic side chain enhances the activity The methylene bridge is necessary for activity In addition, several quinoxaline derivatives are of current interest as anti HCMV agents [28] . Some 2-aryl-2-hydroxy ethylamine substituted 1H,7H-pyrido[1,2,3-de]quinoxaline-6-carboxamides were synthetized and tested ( Figure 6 ). Ganciclovir -0.059 EC 50 : Compound concentration that reduces viral replication by 50%. In addition, several quinoxaline derivatives are of current interest as anti HCMV agents [28] . Some 2-aryl-2-hydroxy ethylamine substituted 1H,7H-pyrido[1,2,3-de]quinoxaline-6-carboxamides were synthetized and tested ( Figure 6 ). In this study, the pyridoquinoxaline nucleus proved to be a useful nucleus, as some of the synthetized compounds showed a favorable profile for established drugs like as ganciclovir, acyclovir, foscarnet, and aphidicolin [28] (Table 4 ), In this study, the pyridoquinoxaline nucleus proved to be a useful nucleus, as some of the synthetized compounds showed a favorable profile for established drugs like as ganciclovir, acyclovir, foscarnet, and aphidicolin [28] (Table 4) , Table 4 . HCMV pol/pra IC 50 for lead compounds. Structure HCMV pol Activity IC 50 (nM) HCMV pra Activity IC 50 (nM) 9 Figure 6 . General structure of 2-aryl-2-hydroxy ethylamine substituted 1H,7H-pyrido[1,2,3de.]quinoxaline-6-carboxamides synthetized. In this study, the pyridoquinoxaline nucleus proved to be a useful nucleus, as some of the synthetized compounds showed a favorable profile for established drugs like as ganciclovir, acyclovir, foscarnet, and aphidicolin [28] (Table 4) , The morpholinomethyl side chain afforded good levels of both enzymatic and antiviral activity whereas the benzofuran moiety resulted in extremely potent enzymatic and antiviral activity. However, despite excellent biological activity, the calculated log P of compound 10 proves the need to continue pharmacomodulation efforts aimed at improving its hydrosolubility. Finally, EBV is a very common virus that can increase the risk of developing certain rare cancers. The malignant transformation of normal human epithelial cells results from exposure to EBV and that transformation is dependent on the presence of phorbol esters, which stimulate cell proliferation through rapid activation of protein kinase C [32, 54] . Novel 3-amioquinoxalin-2(1H)-one derivatives and derivatives with pyrimidine ring linked to quinoxaline through sulfur ( Figure 7 ) exhibited properties against EBV antigen activation. In this study, the pyridoquinoxaline nucleus proved to be a useful nucleus, as some of the synthetized compounds showed a favorable profile for established drugs like as ganciclovir, acyclovir, foscarnet, and aphidicolin [28] (Table 4) , The morpholinomethyl side chain afforded good levels of both enzymatic and antiviral activity whereas the benzofuran moiety resulted in extremely potent enzymatic and antiviral activity. However, despite excellent biological activity, the calculated log P of compound 10 proves the need to continue pharmacomodulation efforts aimed at improving its hydrosolubility. Finally, EBV is a very common virus that can increase the risk of developing certain rare cancers. The malignant transformation of normal human epithelial cells results from exposure to EBV and that transformation is dependent on the presence of phorbol esters, which stimulate cell proliferation through rapid activation of protein kinase C [32, 54] . Novel 3-amioquinoxalin-2(1H)-one derivatives and derivatives with pyrimidine ring linked to quinoxaline through sulfur ( Figure 7 ) exhibited properties against EBV antigen activation. Ganciclovir -1300 Acyclovir ->20,000 The morpholinomethyl side chain afforded good levels of both enzymatic and antiviral activity whereas the benzofuran moiety resulted in extremely potent enzymatic and antiviral activity. However, despite excellent biological activity, the calculated log P of compound 10 proves the need to continue pharmacomodulation efforts aimed at improving its hydrosolubility. Finally, EBV is a very common virus that can increase the risk of developing certain rare cancers. The malignant transformation of normal human epithelial cells results from exposure to EBV and that transformation is dependent on the presence of phorbol esters, which stimulate cell proliferation through rapid activation of protein kinase C [32, 54] . Novel 3-amioquinoxalin-2(1H)-one derivatives and derivatives with pyrimidine ring linked to quinoxaline through sulfur ( Figure 7 ) exhibited properties against EBV antigen activation. On a series of 22 original compounds, six derivatives demonstrated stronger inhibitory effect on EBV than oleanolic acid as reference, without showing any cytotoxicity. The structure-activity relationship proved that disubstitution with alkyl groups on both nitrogen of hydrazine and quinoxaline was crucial for activity especially for the allyl group. This high activity could result from a hydrophobic interaction between the alkyl group and the hydrophobic region of the binding site of the receptor. The presence of a methoxy group on the phenyl group and substitution with a pyrimidine nucleus linked to quinoxaline through sulfur were also conducive to activity [32] . The Poxviridae family include 38 viruses that can infect a wide range of hosts, including On a series of 22 original compounds, six derivatives demonstrated stronger inhibitory effect on EBV than oleanolic acid as reference, without showing any cytotoxicity. The structure-activity relationship proved that disubstitution with alkyl groups on both nitrogen of hydrazine and quinoxaline was crucial for activity especially for the allyl group. This high activity could result from a hydrophobic interaction between the alkyl group and the hydrophobic region of the binding site of the receptor. The presence of a methoxy group on the phenyl group and substitution with a pyrimidine nucleus linked to quinoxaline through sulfur were also conducive to activity [32] . The Poxviridae family include 38 viruses that can infect a wide range of hosts, including mammals, birds, reptiles, and insects [55] . The causative agent of Smallpox and Molluscum contagiosum, two human specific diseases, belongs to the poxviruses. Although variola was globally eradicated, Molluscum contagiosum results from a usually benign infection with mild skin disease characterized by lesions that may appear anywhere on the body. Within 6-12 months, Molluscum contagiosum typically resolves without scarring, but may take as long as many years in some people with weakened immune systems [56] . In a series of nine new halophenyl pyrrolo[2,3-b]quinoxaline derivatives, none of the compounds proved inhibitory at subtoxic concentration except ethyl 2-(4-chlorophenyl)-1-methyl-2,4-dihydro-1H-pyrrolo-[2,3-b]quinoxaline 11 (Figure 8) , which inhibited the vaccinia virus and was considered as a potential lead compound for poxvirus inhibition, with an EC 50 value of 2 µM in HEL cell cultures and moderate antiproliferative activity (CC 50 >20 µM) [33] . pyrimidine ring linked to quinoxaline through sulfur exhibiting anti EBV antigen activation. On a series of 22 original compounds, six derivatives demonstrated stronger inhibitory effect on EBV than oleanolic acid as reference, without showing any cytotoxicity. The structure-activity relationship proved that disubstitution with alkyl groups on both nitrogen of hydrazine and quinoxaline was crucial for activity especially for the allyl group. This high activity could result from a hydrophobic interaction between the alkyl group and the hydrophobic region of the binding site of the receptor. The presence of a methoxy group on the phenyl group and substitution with a pyrimidine nucleus linked to quinoxaline through sulfur were also conducive to activity [32] . The Poxviridae family include 38 viruses that can infect a wide range of hosts, including mammals, birds, reptiles, and insects [55] . The causative agent of Smallpox and Molluscum contagiosum, two human specific diseases, belongs to the poxviruses. Although variola was globally eradicated, Molluscum contagiosum results from a usually benign infection with mild skin disease characterized by lesions that may appear anywhere on the body. Within 6-12 months, Molluscum contagiosum typically resolves without scarring, but may take as long as many years in some people with weakened immune systems [56] . In a series of nine new halophenyl pyrrolo[2,3-b]quinoxaline derivatives, none of the compounds proved inhibitory at subtoxic concentration except ethyl 2-(4-chlorophenyl)-1-methyl-2,4-dihydro-1H-pyrrolo-[2,3-b]quinoxaline 11 (Figure 8) , which inhibited the vaccinia virus and was considered as a potential lead compound for poxvirus inhibition, with an EC50 value of 2 µM in HEL cell cultures and moderate antiproliferative activity (CC50 >20 µM) [33] . Hepatitis B virus (HBV) is a member of the Hepadnaviridae family. This virus can cause liver infections that lead to various hepatic diseases such as hepatitis, cirrhosis, and hepatic cancer. A new class of 3-(1',2'-dihydroxyeth-1'-yl)-1-phenylpyrazolo [3,4-b] quinoxaline demonstrated encouraging anti-hepatitis B activity at 100 µM, but the five most potent compounds were associated with high cytotoxicity (cytotoxicity >30% at 100 µM) [34] . Hepatitis B virus (HBV) is a member of the Hepadnaviridae family. This virus can cause liver infections that lead to various hepatic diseases such as hepatitis, cirrhosis, and hepatic cancer. A new class of 3-(1 ,2 -dihydroxyeth-1 -yl)-1-phenylpyrazolo [3,4-b] quinoxaline demonstrated encouraging anti-hepatitis B activity at 100 µM, but the five most potent compounds were associated with high cytotoxicity (cytotoxicity >30% at 100 µM) [34] . Human disease-causing RNA viruses include Orthomyxoviruses, Hepatitis C Virus (HCV), Ebola disease, SARS, and retroviruses including adult human T-cell lymphotropic virus type 1 (HTLV-1) and human immunodeficiency virus (HIV). RNA viruses have RNA as genetic material, either single-stranded RNA or double-stranded RNA. Viruses may exploit the presence of RNA-dependent RNA polymerases for replication of their genomes or, in retroviruses, reverse transcriptase produces viral DNA, which can be integrated into the host DNA under its integrase function [57] . Enteroviruses, which include coxsackievirus A and B, belong to Picornaviridae, a single-stranded RNA virus family. They are implicated in various diseases, with a wide range of symptoms; and exceptionally, coxsakieviruses can be responsible for more severe diseases, such as flaccid paralysis myocarditis, pericarditis, encephalitis, or systemic neonatal disease [35, 58, 59] . To date, there are conventional treatments or vaccines against coxsackieviruses, which cause acute or chronic disease in infants, children, and immunocompromised persons. In order to develop more effective antivirals, 14 new quinoxaline derivatives (Figure 9 ) were synthetized and tested against a panel of viruses for which the efficacy of therapeutic agents was unsatisfactory [35] . Enteroviruses, which include coxsackievirus A and B, belong to Picornaviridae, a single-stranded RNA virus family. They are implicated in various diseases, with a wide range of symptoms; and exceptionally, coxsakieviruses can be responsible for more severe diseases, such as flaccid paralysis myocarditis, pericarditis, encephalitis, or systemic neonatal disease [35, 58, 59] . To date, there are conventional treatments or vaccines against coxsackieviruses, which cause acute or chronic disease in infants, children, and immunocompromised persons. In order to develop more effective antivirals, 14 new quinoxaline derivatives ( Figure 9 ) were synthetized and tested against a panel of viruses for which the efficacy of therapeutic agents was unsatisfactory [35] . Table 5 ). The absence of cytotoxicity towards the Vero-76 cells of compound 11 led to further experimental/in silico investigation aimed at determining its mechanism of action. These investigations demonstrated that compound 11 inhibits CBV5 by targeting the early events of attachment, entry or uncoating, as it can favorably insert into a hydrophobic pocket on the VP1 chain of the capsid protomer implicated in the protein conformational changes during infection of the host cell. Table 5 ). The absence of cytotoxicity towards the Vero-76 cells of compound 11 led to further experimental/in silico investigation aimed at determining its mechanism of action. These investigations demonstrated that compound 11 inhibits CBV5 by targeting the early events of attachment, entry or uncoating, as it can favorably insert into a hydrophobic pocket on the VP1 chain of the capsid protomer implicated in the protein conformational changes during infection of the host cell. Influenza viruses can cause contagious respiratory disease in humans and are responsible every year for flu pandemics. Based on the planar polyaromatic system (chromophore), quinoxaline derivatives are good candidates to combat influenza viruses because of their potential to target the NS1 protein, a highly conserved influenza virus encoded protein. Since the N-terminal domain of the NS1A protein results in a six-helical chain fold with a deep cavity at the center of the double-stranded RNA-binding surface, a small molecule could fit into the cavity and block virus replication [36, [60] [61] [62] . 2,3,6substitued quinoxaline has also yielded compounds identified as having valuable antiviral activity, particularly under bis-2-furyl substitution. Maintaining bis-2-furyl substitution, a novel series of quinoxaline derivatives was synthetized to determine the influence of substitution at position 6. Two derivatives, one with 3-methoxyphenyl group and one with 2-furyl at position 6, showed good activity with an IC50 of 6.2 and 3.5 µM, respectively (Table 6 ). RNA intercalation experiments showed that both compounds could bind to the NS1A RNA-binding domain, demonstrating the antiviral potential of these quinoxaline derivatives [36] . Influenza viruses can cause contagious respiratory disease in humans and are responsible every year for flu pandemics. Based on the planar polyaromatic system (chromophore), quinoxaline derivatives are good candidates to combat influenza viruses because of their potential to target the NS1 protein, a highly conserved influenza virus encoded protein. Since the N-terminal domain of the NS1A protein results in a six-helical chain fold with a deep cavity at the center of the double-stranded RNA-binding surface, a small molecule could fit into the cavity and block virus replication [36, [60] [61] [62] . 2,3,6substitued quinoxaline has also yielded compounds identified as having valuable antiviral activity, particularly under bis-2-furyl substitution. Maintaining bis-2-furyl substitution, a novel series of quinoxaline derivatives was synthetized to determine the influence of substitution at position 6. Two derivatives, one with 3-methoxyphenyl group and one with 2-furyl at position 6, showed good activity with an IC50 of 6.2 and 3.5 µM, respectively (Table 6 ). RNA intercalation experiments showed that both compounds could bind to the NS1A RNA-binding domain, demonstrating the antiviral potential of these quinoxaline derivatives [36] . Influenza viruses can cause contagious respiratory disease in humans and are responsible every year for flu pandemics. Based on the planar polyaromatic system (chromophore), quinoxaline derivatives are good candidates to combat influenza viruses because of their potential to target the NS1 protein, a highly conserved influenza virus encoded protein. Since the N-terminal domain of the NS1A protein results in a six-helical chain fold with a deep cavity at the center of the double-stranded RNA-binding surface, a small molecule could fit into the cavity and block virus replication [36, [60] [61] [62] . 2,3,6substitued quinoxaline has also yielded compounds identified as having valuable antiviral activity, particularly under bis-2-furyl substitution. Maintaining bis-2-furyl substitution, a novel series of quinoxaline derivatives was synthetized to determine the influence of substitution at position 6. Two derivatives, one with 3-methoxyphenyl group and one with 2-furyl at position 6, showed good activity with an IC50 of 6.2 and 3.5 µM, respectively (Table 6 ). RNA intercalation experiments showed that both compounds could bind to the NS1A RNA-binding domain, demonstrating the antiviral potential of these quinoxaline derivatives [36] . Influenza viruses can cause contagious respiratory disease in humans and are responsible every year for flu pandemics. Based on the planar polyaromatic system (chromophore), quinoxaline derivatives are good candidates to combat influenza viruses because of their potential to target the NS1 protein, a highly conserved influenza virus encoded protein. Since the N-terminal domain of the NS1A protein results in a six-helical chain fold with a deep cavity at the center of the double-stranded RNA-binding surface, a small molecule could fit into the cavity and block virus replication [36, [60] [61] [62] . 2,3,6-substitued quinoxaline has also yielded compounds identified as having valuable antiviral activity, particularly under bis-2-furyl substitution. Maintaining bis-2-furyl substitution, a novel series of quinoxaline derivatives was synthetized to determine the influence of substitution at position 6. Two derivatives, one with 3-methoxyphenyl group and one with 2-furyl at position 6, showed good activity with an IC 50 of 6.2 and 3.5 µM, respectively (Table 6 ). RNA intercalation experiments showed that both compounds could bind to the NS1A RNA-binding domain, demonstrating the antiviral potential of these quinoxaline derivatives [36] . Table 6 . Structure and activity of 2,3,6-substitued quinoxaline. Structure % Binding at 50 µM % Intercalation at 50 µM year for flu pandemics. Based on the planar polyaromatic system (chromophore), quinoxaline derivatives are good candidates to combat influenza viruses because of their potential to target the NS1 protein, a highly conserved influenza virus encoded protein. Since the N-terminal domain of the NS1A protein results in a six-helical chain fold with a deep cavity at the center of the double-stranded RNA-binding surface, a small molecule could fit into the cavity and block virus replication [36, [60] [61] [62] . 2,3,6substitued quinoxaline has also yielded compounds identified as having valuable antiviral activity, particularly under bis-2-furyl substitution. Maintaining bis-2-furyl substitution, a novel series of quinoxaline derivatives was synthetized to determine the influence of substitution at position 6. Two derivatives, one with 3-methoxyphenyl group and one with 2-furyl at position 6, showed good activity with an IC50 of 6.2 and 3.5 µM, respectively (Table 6 ). RNA intercalation experiments showed that both compounds could bind to the NS1A RNA-binding domain, demonstrating the antiviral potential of these quinoxaline derivatives [36] . Table 6 . Structure and activity of 2,3,6-substitued quinoxaline. Influenza viruses can cause contagious respiratory disease in humans and are responsible every year for flu pandemics. Based on the planar polyaromatic system (chromophore), quinoxaline derivatives are good candidates to combat influenza viruses because of their potential to target the NS1 protein, a highly conserved influenza virus encoded protein. Since the N-terminal domain of the NS1A protein results in a six-helical chain fold with a deep cavity at the center of the double-stranded RNA-binding surface, a small molecule could fit into the cavity and block virus replication [36, [60] [61] [62] . 2,3,6substitued quinoxaline has also yielded compounds identified as having valuable antiviral activity, particularly under bis-2-furyl substitution. Maintaining bis-2-furyl substitution, a novel series of quinoxaline derivatives was synthetized to determine the influence of substitution at position 6. Two derivatives, one with 3-methoxyphenyl group and one with 2-furyl at position 6, showed good activity with an IC50 of 6.2 and 3.5 µM, respectively (Table 6 ). RNA intercalation experiments showed that both compounds could bind to the NS1A RNA-binding domain, demonstrating the antiviral potential of these quinoxaline derivatives [36] . Ebola and Marburg belong to the Filoviridae family of single-stranded RNA viruses. These viruses were responsible for the 2014-2015 outbreak of hemorrhagic fever in Western Africa, resulting in a total of 28,616 infected people, including 11,310 deaths, for a case-fatality rate of 40%. There is currently no antiviral drug licensed by the U.S. Food and Drug Administration (FDA) to treat Ebola infection; however, four drugs called ZMapp remdesivir, Mab114, and REGN-EB3, are under investigation, as each has reduced the risk of death from Ebola [63, 64] . Actually, the outbreak is not yet over, with new cases identified in the Democratic Republic of Congo, and the need for antiviral candidates remains strong. A critical virus-host interaction required for virus egress and dissemination involves late-budding domains containing PPxY motifs, which are highly conserved in the matrix protein of a large number of RNA viruses. Targeting this interaction, a novel series of quinoxaline-2-mercapto-acetyl-urea analogues ( Figure 10 ) were synthetized and evaluated for their ability to inhibit viral egress of Marburg and Ebola in VP40 VLP budding assay in HEK293T cells [2] . Among them, four compounds demonstrated strong RNA viral egress inhibition potential. Molecules 2020, 25, x FOR PEER REVIEW 11 of 20 Ebola and Marburg belong to the Filoviridae family of single-stranded RNA viruses. These viruses were responsible for the 2014-2015 outbreak of hemorrhagic fever in Western Africa, resulting in a total of 28,616 infected people, including 11,310 deaths, for a case-fatality rate of 40%. There is currently no antiviral drug licensed by the U.S. Food and Drug Administration (FDA) to treat Ebola infection; however, four drugs called ZMapp remdesivir, Mab114, and REGN-EB3, are under investigation, as each has reduced the risk of death from Ebola [63, 64] . Actually, the outbreak is not yet over, with new cases identified in the Democratic Republic of Congo, and the need for antiviral candidates remains strong. A critical virus-host interaction required for virus egress and dissemination involves latebudding domains containing PPxY motifs, which are highly conserved in the matrix protein of a large number of RNA viruses. Targeting this interaction, a novel series of quinoxaline-2-mercapto-acetylurea analogues ( Figure 10 ) were synthetized and evaluated for their ability to inhibit viral egress of Marburg and Ebola in VP40 VLP budding assay in HEK293T cells [2] . Among them, four compounds demonstrated strong RNA viral egress inhibition potential. HCV is responsible for both acute and chronic hepatitis, ranging in severity from a mild illness lasting a few weeks to a serious, lifelong illness. Globally, an estimated 71 million people have chronic HCV infection and in 2016, approximately 399,000 people died from HCV [65] . Antiviral drugs can cure more than 95% of HCV patients, but access to treatment is poor due to its high cost, which is why research is ongoing. Several quinoxaline derivatives were evaluated for their anti-HCV potential. Even though novel quinoxaline derivatives synthetized using ethyl (6,7-dimethyl-2-oxo-3,4-dihydroquinoxalin-3-yl)acetate 4 and ethyl (6-methyl-2-oxo-3,4-dihydroquinoxalin-3-yl)acetate 5, 3-methylquinoxalin-2(1H)-one, and 1,4-dihydroquinoxaline-2,3-dione as precursors failed to demonstrate any activity against HCV [31] , in pyrido[2,3-g]quinoxalinone series, 5-chloro-3-(thiophen-2-yl)pyrido[2,3-g]quinoxaline-2(1H)-one 16 ( Figure 11 ) was able to inhibit HCV replication in a subgenomic replication assay with EC 50 = 7.5 ± 0.5 µM. However, it was also cytotoxic for GS4.1 cells (CC 50 = 21 ± 20 µM) [37, 38, 55] . quinoxaline moiety Figure 10 . Structure-activity relationship of novel series of quinoxaline-2-mercapto-acetyl-urea analogues. HCV is responsible for both acute and chronic hepatitis, ranging in severity from a mild illness lasting a few weeks to a serious, lifelong illness. Globally, an estimated 71 million people have chronic HCV infection and in 2016, approximately 399,000 people died from HCV [65] . Antiviral drugs can cure more than 95% of HCV patients, but access to treatment is poor due to its high cost, which is why research is ongoing. Several quinoxaline derivatives were evaluated for their anti-HCV potential. Even though novel quinoxaline derivatives synthetized using ethyl (6,7-dimethyl-2-oxo-3,4-dihydroquinoxalin-3yl)acetate 4 and ethyl (6-methyl-2-oxo-3,4-dihydroquinoxalin-3-yl)acetate 5, 3-methylquinoxalin-2(1H)-one, and 1,4-dihydroquinoxaline-2,3-dione as precursors failed to demonstrate any activity against HCV [31] , in pyrido[2,3-g]quinoxalinone series, 5-chloro-3-(thiophen-2-yl)pyrido[2,3g]quinoxaline-2(1H)-one 16 ( Figure 11 ) was able to inhibit HCV replication in a subgenomic replication assay with EC50 = 7.5 ± 0.5 µM. However, it was also cytotoxic for GS4.1 cells (CC50 = 21 ± 20 µM) [37, 38, 55] . However, grazoprevir 17, a novel P2-P4 quinoxaline macrocyclic NS3/4a protease inhibitor with broad activity across genotypes and resistant variants, is currently approved for the treatment for HCV [39] (Figure 12 ). However, grazoprevir 17, a novel P2-P4 quinoxaline macrocyclic NS3/4a protease inhibitor with broad activity across genotypes and resistant variants, is currently approved for the treatment for HCV [39] (Figure 12) . The structure-activity relationship shows that grazoprevir's efficacy derives from lipophilic interaction at P2 position in addition to a contribution from the P2-P4 constraint [66] . The P2 quinoxaline moiety largely avoids direct interaction with residues Arg-155 and Asp-168, the two most common resistance-associated residues, but interacts with the catalytic His-57 and Asp-81, which explains its activity against most HCV genotypes and resistant variants [67] . Modeling studies showed that in patients who failed to achieve sustained virologic response with simeprevir, grazoprevir was efficacious because of a strong direct cation-quinoxaline interaction with the Lys-155 side chain of double substitution R155K/D168A [40] . More recently, eliminating the P2-P4 moiety was considered aiming at conformational flexibility and exploration of diverse quinoxalines at position P2 in order to improve potency and resistance profile. The structure-activity relationship indicated that a small hydrophobic substituent at position 3 of P2 quinoxaline effectively maintains activity against resistant variants, as derivatives with a larger group at position 3 of P2 quinoxaline shift out of the binding site [41, 42] . Further investigations involved replacement of the quinoxaline moiety by a quinoline scaffold, leading to interesting analogues [68] . Vesicular stomatis virus (VSV) is a virus in the family Rhabdoviridae. This virus is zoonotic and in infected humans leads to a flu-like illness characterized by fever, headache, myalgia, weakness, The structure-activity relationship shows that grazoprevir's efficacy derives from lipophilic interaction at P2 position in addition to a contribution from the P2-P4 constraint [66] . The P2 quinoxaline moiety largely avoids direct interaction with residues Arg-155 and Asp-168, the two most common resistance-associated residues, but interacts with the catalytic His-57 and Asp-81, which explains its activity against most HCV genotypes and resistant variants [67] . Modeling studies showed that in patients who failed to achieve sustained virologic response with simeprevir, grazoprevir was efficacious because of a strong direct cation-quinoxaline interaction with the Lys-155 side chain of double substitution R155K/D168A [40] . More recently, eliminating the P2-P4 moiety was considered aiming at conformational flexibility and exploration of diverse quinoxalines at position P2 in order to improve potency and resistance profile. The structure-activity relationship indicated that a small hydrophobic substituent at position 3 of P2 quinoxaline effectively maintains activity against resistant variants, as derivatives with a larger group at position 3 of P2 quinoxaline shift out of the binding site [41, 42] . Further investigations involved replacement of the quinoxaline moiety by a quinoline scaffold, leading to interesting analogues [68] . Vesicular stomatis virus (VSV) is a virus in the family Rhabdoviridae. This virus is zoonotic and in infected humans leads to a flu-like illness characterized by fever, headache, myalgia, weakness, and occasionally vesicular lesions of the mouth [69] . As VSV infection results in a short 3-5-day illness, no specific treatment is available and VSV is commonly used as a laboratory virus to study the properties of viruses. Indoloquinoxaline derivatives and their benzoindoloquinoxalines were synthetized and assessed for their anti-VSV activity, interferon-inducing ability, and cytotoxicity ( Figure 13 ). Anti-viral activity was significantly reduced with annulation of benzene ring in indoloquinoxaline derivatives [43, 44] . showed that in patients who failed to achieve sustained virologic response with simeprevir, grazoprevir was efficacious because of a strong direct cation-quinoxaline interaction with the Lys-155 side chain of double substitution R155K/D168A [40] . More recently, eliminating the P2-P4 moiety was considered aiming at conformational flexibility and exploration of diverse quinoxalines at position P2 in order to improve potency and resistance profile. The structure-activity relationship indicated that a small hydrophobic substituent at position 3 of P2 quinoxaline effectively maintains activity against resistant variants, as derivatives with a larger group at position 3 of P2 quinoxaline shift out of the binding site [41, 42] . Further investigations involved replacement of the quinoxaline moiety by a quinoline scaffold, leading to interesting analogues [68] . Vesicular stomatis virus (VSV) is a virus in the family Rhabdoviridae. This virus is zoonotic and in infected humans leads to a flu-like illness characterized by fever, headache, myalgia, weakness, and occasionally vesicular lesions of the mouth [69] . As VSV infection results in a short 3-5-day illness, no specific treatment is available and VSV is commonly used as a laboratory virus to study the properties of viruses. Indoloquinoxaline derivatives and their benzoindoloquinoxalines were synthetized and assessed for their anti-VSV activity, interferon-inducing ability, and cytotoxicity ( Figure 13 ). Anti-viral activity was significantly reduced with annulation of benzene ring in indoloquinoxaline derivatives [43, 44] . As these indoloquinoxalines were more active antivirals when they were added immediately after virus infection, it was supposed that their antiviral action was first mediated by interferon. As these indoloquinoxalines were more active antivirals when they were added immediately after virus infection, it was supposed that their antiviral action was first mediated by interferon. While there is no cure for HIV, there are very effective treatments that enable most people with the virus to live a long and healthy life. Combination antiretroviral therapy is required for durable virologic suppression. Reverse transcriptase is one of the most frequent targets for the treatment of HIV infection, since the blockage of this enzyme can stop an essential step in viral replication. However, a growing number of cases of resistant HIV strains and serious adverse events due to the antiretroviral therapy administered have encouraged attempts to develop new HIV agents, more active, less toxic, and with increased tolerability to mutation [70] . Some quinoxaline derivatives like HBY, HBQ, and S-2720 have demonstrated high potency as reverse transcriptase inhibitors ( Figure 14) . For these reasons, design, synthesis, and evaluation of new quinoxaline derivatives was investigated. Using a computational approach, 58 quinoxaline compounds were identified, and 25 new quinoxaline and quinoxaline-related compounds were synthetized and evaluated as inhibitors of reverse transcriptase (RT). Chemical features identified as crucial for reverse transcriptase inhibition were the presence of a five-or six-membered aromatic ring and a hydrophilic center that can be nitrogen, oxygen, or sulfur. Six of these derivatives presented the highest inhibitory activity at 100 µM, ranging from 56% to 99% of reverse transcriptase inhibition, and were considered as hit compounds. One compound was a particularly interesting derivative, with values comparable to those of commercial compound nevirapine when used at 10 µM (both showing reverse transcriptase inhibition % = 91) [45] (Table 7) . For these reasons, design, synthesis, and evaluation of new quinoxaline derivatives was investigated. Using a computational approach, 58 quinoxaline compounds were identified, and 25 new quinoxaline and quinoxaline-related compounds were synthetized and evaluated as inhibitors of reverse transcriptase (RT). Chemical features identified as crucial for reverse transcriptase inhibition were the presence of a five-or six-membered aromatic ring and a hydrophilic center that can be nitrogen, oxygen, or sulfur. Six of these derivatives presented the highest inhibitory activity at 100 µM, ranging from 56% to 99% of reverse transcriptase inhibition, and were considered as hit compounds. One compound was a particularly interesting derivative, with values comparable to those of commercial compound nevirapine when used at 10 µM (both showing reverse transcriptase inhibition % = 91) [45] (Table 7 ). Compound 19 displayed similar inhibitory activity with nevirapine, with an EC50 = 3.1 nM vs. EC50 = 6.7 nM respectively and can be considered a promising lead compound [45] . Moreover, this new class of integrase inhibitors proved very effective against HIV, showing a high therapeutic index. Three representatives of this class, raltegravir, elvitegravir, and dolutegravir are currently available. However, for two of them have led to reported cases of resistance, indicating to an urgent need to develop other new effective anti-HIV agents. In a structure-based drug design approach, the quinoxaline scaffold was identified as a core moiety to design potential novel anti-HIV agents. A series of seven new 6-chloro-7-fluoroquinoxaline derivatives with various substituents at position 2 and 3 was also synthetized. Among them, two derivatives 23 and 24 bearing bulky substitution at position 2 and 3 exhibited better activity compared to unsubstituted or less bulky substitutions [46] . In addition, these two compounds revealed no cytotoxicity on VERO cells (Table 8) . 56 7 NE NE Molecules 2020, 25, x FOR PEER REVIEW 14 of 20 Compound 19 displayed similar inhibitory activity with nevirapine, with an EC50 = 3.1 nM vs. EC50 = 6.7 nM respectively and can be considered a promising lead compound [45] . Moreover, this new class of integrase inhibitors proved very effective against HIV, showing a high therapeutic index. Three representatives of this class, raltegravir, elvitegravir, and dolutegravir are currently available. However, for two of them have led to reported cases of resistance, indicating to an urgent need to develop other new effective anti-HIV agents. In a structure-based drug design approach, the quinoxaline scaffold was identified as a core moiety to design potential novel anti-HIV agents. A series of seven new 6-chloro-7-fluoroquinoxaline derivatives with various substituents at position 2 and 3 was also synthetized. Among them, two derivatives 23 and 24 bearing bulky substitution at position 2 and 3 exhibited better activity compared to unsubstituted or less bulky substitutions [46] . In addition, these two compounds revealed no cytotoxicity on VERO cells (Table 8) . Compound 19 displayed similar inhibitory activity with nevirapine, with an EC50 = 3.1 nM vs. EC50 = 6.7 nM respectively and can be considered a promising lead compound [45] . Moreover, this new class of integrase inhibitors proved very effective against HIV, showing a high therapeutic index. Three representatives of this class, raltegravir, elvitegravir, and dolutegravir are currently available. However, for two of them have led to reported cases of resistance, indicating to an urgent need to develop other new effective anti-HIV agents. In a structure-based drug design approach, the quinoxaline scaffold was identified as a core moiety to design potential novel anti-HIV agents. A series of seven new 6-chloro-7-fluoroquinoxaline derivatives with various substituents at position 2 and 3 was also synthetized. Among them, two derivatives 23 and 24 bearing bulky substitution at position 2 and 3 exhibited better activity compared to unsubstituted or less bulky substitutions [46] . In addition, these two compounds revealed no cytotoxicity on VERO cells (Table 8) . Compound 19 displayed similar inhibitory activity with nevirapine, with an EC50 = 3.1 nM vs. EC50 = 6.7 nM respectively and can be considered a promising lead compound [45] . Moreover, this new class of integrase inhibitors proved very effective against HIV, showing a high therapeutic index. Three representatives of this class, raltegravir, elvitegravir, and dolutegravir are currently available. However, for two of them have led to reported cases of resistance, indicating to an urgent need to develop other new effective anti-HIV agents. In a structure-based drug design approach, the quinoxaline scaffold was identified as a core moiety to design potential novel anti-HIV agents. A series of seven new 6-chloro-7-fluoroquinoxaline derivatives with various substituents at position 2 and 3 was also synthetized. Among them, two derivatives 23 and 24 bearing bulky substitution at position 2 and 3 exhibited better activity compared to unsubstituted or less bulky substitutions [46] . In addition, these two compounds revealed no cytotoxicity on VERO cells (Table 8) . Compound 19 displayed similar inhibitory activity with nevirapine, with an EC50 = 3.1 nM vs. EC50 = 6.7 nM respectively and can be considered a promising lead compound [45] . Moreover, this new class of integrase inhibitors proved very effective against HIV, showing a high therapeutic index. Three representatives of this class, raltegravir, elvitegravir, and dolutegravir are currently available. However, for two of them have led to reported cases of resistance, indicating to an urgent need to develop other new effective anti-HIV agents. In a structure-based drug design approach, the quinoxaline scaffold was identified as a core moiety to design potential novel anti-HIV agents. A series of seven new 6-chloro-7-fluoroquinoxaline derivatives with various substituents at position 2 and 3 was also synthetized. Among them, two derivatives 23 and 24 bearing bulky substitution at position 2 and 3 exhibited better activity compared to unsubstituted or less bulky substitutions [46] . In addition, these two compounds revealed no cytotoxicity on VERO cells (Table 8) . Compound 19 displayed similar inhibitory activity with nevirapine, with an EC 50 = 3.1 nM vs. EC 50 = 6.7 nM respectively and can be considered a promising lead compound [45] . Moreover, this new class of integrase inhibitors proved very effective against HIV, showing a high therapeutic index. Three representatives of this class, raltegravir, elvitegravir, and dolutegravir are currently available. However, for two of them have led to reported cases of resistance, indicating to an urgent need to develop other new effective anti-HIV agents. In a structure-based drug design approach, the quinoxaline scaffold was identified as a core moiety to design potential novel anti-HIV agents. A series of seven new 6-chloro-7-fluoroquinoxaline derivatives with various substituents at position 2 and 3 was also synthetized. Among them, two derivatives 23 and 24 bearing bulky substitution at position 2 and 3 exhibited better activity compared to unsubstituted or less bulky substitutions [46] . In addition, these two compounds revealed no cytotoxicity on VERO cells (Table 8) . Table 8 . HIV activity and cytotoxicity study of the two most potent new 6-chloro-7fluoroquinoxaline derivatives. Recently, an approach aimed at dysregulation of gelatinase and pathogenesis of HIV led to the synthesis of two new classes of gelatinase inhibitors bearing a quinoxalinone motif, based on this coplanar scaffold being able to penetrate into the relatively broad S1 binding domain of gelatinase. The acylamide (Series I) and acylhydrazone (Series II) linkage can also act as potent H-bonding acceptor/donor to interact with the active amino acid of the enzyme [47] (Figure 15 ). Derivatives in series 1 displayed moderate activity with gelatinase enzymatic inhibition ranging from 34.79 ± 6.3 µM to >500 µM against 5.64 ± 0.6 µM for LY52 taken as reference. The best activity was observed for a para-chloro phenyl substituent. In series II, two derivatives 25 and 26 demonstrated similar activity with LY52, probably because the substituents introduced have enough space and the right orientation to guide the compounds to fit into the binding cavity (Table 9 ). In addition, as compound 26 displayed slightly more potent activity than compound 25, it was concluded that the phenolic hydroxyl group could provide a more effective hydrogen-bonding interaction, resulting in increased affinity. Substitution with an aliphatic group led to inactive compounds. Molecules 2020, 25, x FOR PEER REVIEW 15 of 20 Recently, an approach aimed at dysregulation of gelatinase and pathogenesis of HIV led to the synthesis of two new classes of gelatinase inhibitors bearing a quinoxalinone motif, based on this coplanar scaffold being able to penetrate into the relatively broad S1 binding domain of gelatinase. The acylamide (Series I) and acylhydrazone (Series II) linkage can also act as potent H-bonding acceptor/donor to interact with the active amino acid of the enzyme [47] (Figure 15 ). Derivatives in series 1 displayed moderate activity with gelatinase enzymatic inhibition ranging from 34.79 ± 6.3 µM to >500 µM against 5.64 ± 0.6 µM for LY52 taken as reference. The best activity was observed for a para-chloro phenyl substituent. In series II, two derivatives 25 and 26 demonstrated similar activity with LY52, probably because the substituents introduced have enough space and the right orientation to guide the compounds to fit into the binding cavity (Table 9 ). In addition, as compound 26 displayed slightly more potent activity than compound 25, it was concluded that the phenolic hydroxyl group could provide a more effective hydrogen-bonding interaction, resulting in increased affinity. Substitution with an aliphatic group led to inactive compounds. Recently, an approach aimed at dysregulation of gelatinase and pathogenesis of HIV led to the synthesis of two new classes of gelatinase inhibitors bearing a quinoxalinone motif, based on this coplanar scaffold being able to penetrate into the relatively broad S1 binding domain of gelatinase. The acylamide (Series I) and acylhydrazone (Series II) linkage can also act as potent H-bonding acceptor/donor to interact with the active amino acid of the enzyme [47] (Figure 15 ). Recently, an approach aimed at dysregulation of gelatinase and pathogenesis of HIV led to the synthesis of two new classes of gelatinase inhibitors bearing a quinoxalinone motif, based on this coplanar scaffold being able to penetrate into the relatively broad S1 binding domain of gelatinase. The acylamide (Series I) and acylhydrazone (Series II) linkage can also act as potent H-bonding acceptor/donor to interact with the active amino acid of the enzyme [47] (Figure 15 ). Derivatives in series 1 displayed moderate activity with gelatinase enzymatic inhibition ranging from 34.79 ± 6.3 µM to >500 µM against 5.64 ± 0.6 µM for LY52 taken as reference. The best activity was observed for a para-chloro phenyl substituent. In series II, two derivatives 25 and 26 demonstrated similar activity with LY52, probably because the substituents introduced have enough space and the right orientation to guide the compounds to fit into the binding cavity (Table 9 ). In addition, as compound 26 displayed slightly more potent activity than compound 25, it was concluded that the phenolic hydroxyl group could provide a more effective hydrogen-bonding interaction, resulting in increased affinity. Substitution with an aliphatic group led to inactive compounds. Derivatives in series 1 displayed moderate activity with gelatinase enzymatic inhibition ranging from 34.79 ± 6.3 µM to >500 µM against 5.64 ± 0.6 µM for LY52 taken as reference. The best activity was observed for a para-chloro phenyl substituent. In series II, two derivatives 25 and 26 demonstrated similar activity with LY52, probably because the substituents introduced have enough space and the right orientation to guide the compounds to fit into the binding cavity (Table 9 ). In addition, as compound 26 displayed slightly more potent activity than compound 25, it was concluded that the phenolic hydroxyl group could provide a more effective hydrogen-bonding interaction, resulting in increased affinity. Substitution with an aliphatic group led to inactive compounds. Quinoxaline represent an important class of nitrogen-containing heterocycles with a wide range of potential biological activities. This review points to a growing interest in the development of compounds bearing a quinoxaline moiety for antiviral treatment. Regarding the antiviral activity of quinoxaline derivatives, studies showed that these derivatives represented very encouraging agents for investigators as they exhibit some activity against a large number of different viruses. Future investigations of this moiety requiring analysis of structure-activity relationships, as well as the mechanisms of action of these compounds could give some more encouraging results and may provide to new useful therapeutics. 9.39 ± 0.7 >100 Molecules 2020, 25, x FOR PEER REVIEW 16 of 20 Quinoxaline represent an important class of nitrogen-containing heterocycles with a wide range of potential biological activities. This review points to a growing interest in the development of compounds bearing a quinoxaline moiety for antiviral treatment. Regarding the antiviral activity of quinoxaline derivatives, studies showed that these derivatives represented very encouraging agents for investigators as they exhibit some activity against a large number of different viruses. Future investigations of this moiety requiring analysis of structure-activity relationships, as well as the mechanisms of action of these compounds could give some more encouraging results and may provide to new useful therapeutics. 7.17 ± 0.95 >100 5.64 ± 0.6 >100 Quinoxaline represent an important class of nitrogen-containing heterocycles with a wide range of potential biological activities. This review points to a growing interest in the development of compounds bearing a quinoxaline moiety for antiviral treatment. Regarding the antiviral activity of quinoxaline derivatives, studies showed that these derivatives represented very encouraging agents for investigators as they exhibit some activity against a large number of different viruses. Future investigations of this moiety requiring analysis of structure-activity relationships, as well as the mechanisms of action of these compounds could give some more encouraging results and may provide to new useful therapeutics. Current Landscape of Antiviral Drug Discovery Quinoxaline-based inhibitors of Ebola and Marburg VP40 egress Remdesivir, Sofosbuvir, Galidesivir, and Tenofovir against SARS-CoV-2 RNA dependent RNA polymerase (RdRp): A molecular docking study Daclatasvir-sofosbuvir combination therapy with or without ribavirin for hepatitis C virus infection: From the clinical trials to real life An insight into the therapeutic potential of quinazoline derivatives as anticancer agents Recent insight into the biological activities of the synthetic xanthone derivatives Recent developments in steroidal and nonsteroidal aromatase inhibitors for the chemoprevention of oestrogen-dependent breast cancer An important class of organic compounds with diverse biological activities Biological activity of quinoxaline derivatives Current Landscape of Antiviral Drug Discovery Quinoxaline-based inhibitors of Ebola and Marburg VP40 egress Remdesivir, Sofosbuvir, Galidesivir, and Tenofovir against SARS-CoV-2 RNA dependent RNA polymerase (RdRp): A molecular docking study Daclatasvir-sofosbuvir combination therapy with or without ribavirin for hepatitis C virus infection: From the clinical trials to real life An insight into the therapeutic potential of quinazoline derivatives as anticancer agents Recent insight into the biological activities of the synthetic xanthone derivatives Recent developments in steroidal and nonsteroidal aromatase inhibitors for the chemoprevention of oestrogen-dependent breast cancer An important class of organic compounds with diverse biological activities Biological activity of quinoxaline derivatives Current Landscape of Antiviral Drug Discovery Quinoxaline-based inhibitors of Ebola and Marburg VP40 egress Remdesivir, Sofosbuvir, Galidesivir, and Tenofovir against SARS-CoV-2 RNA dependent RNA polymerase (RdRp): A molecular docking study Daclatasvir-sofosbuvir combination therapy with or without ribavirin for hepatitis C virus infection: From the clinical trials to real life An insight into the therapeutic potential of quinazoline derivatives as anticancer agents Recent insight into the biological activities of the synthetic xanthone derivatives Recent developments in steroidal and nonsteroidal aromatase inhibitors for the chemoprevention of oestrogen-dependent breast cancer An important class of organic compounds with diverse biological activities Biological activity of quinoxaline derivatives Quinoxaline: An insight into the recent pharmacological advances Condensed Pyrazines Quinoxaline, its derivative and applications: A state of the art review The Chemistry of Heterocyclic Compounds Straightforward access to pyrazines, piperazinones, and quinoxalines by reactions of 1,2-diaza-1,3-butadienes with 1,2-diamines under solution, solvent-free, or solid-phase conditions An efficient synthesis of quinoxalines catalyzed by monoammonium salt of 12-tungstophosphoric acid Direct and catalytic synthesis of quinoxaline derivatives from epoxides and ene-1,2-diamines Gallium triflate catalyzed synthesis of quinoxaline derivatives One-pot efficient green synthesis of 1,4-dihydroquinoxaline-2,3-dione derivatives Synthesis of potential chemotherapic quinoxalinone derivatives by biocatalysis or microwave-assisted Hinserg reaction The synthesis of quinoxalines under microwave irradiation Recent developments in the synthesis of quinoxaline derivatives by green synthetic approaches The mechanism of action of the anti-herpes virus compound 2,3-dimethyl-6(2-dimethylaminoethyl)-6H-indolo-(2,3-b)quinoxaline Antitumoral activity of quinoxaline derivatives: A systematic review Quinoxaline derivatives as potential antitrypanosomal and antileishmanialagents Quinoxaline derivatives: A patent review (2006-present) Activity of a novel quinoxaline derivative against human immunodeficiency virus type 1 reverse transcriptase and viral replication Preferred Reporting Items for Systematic Reviews and Meta-Analyses: The PRISMA Statement The design and development of 2-aryl-2-hydroxy ethylamine substituted 1H,7H-pyrido[1,2,3-de]quinoxaline-6-carboxamides as inhibitors of human cytomegalovirus polymerase ] quinoxaline: Synthesis, antiviral, and antimicrobial activities Synthesis, antioxidant, anticancer and antiviral activities of novel quinoxaline hydrazone derivatives and their acyclic C-nucleosides Synthesis, Characterization, and Biological Evaluation of Some Novel Quinoxaline Derivatives as Antiviral Agents Part I: Synthesis, cancer chemopreventive activity and molecular docking study of novel quinoxaline derivatives A novel and easy two-step, microwave-assisted method for the synthesis of halophenyl pyrrolo[2,3-b]quinoxalines via their pyrrolo precursors Synthesis and novel chemical reaction for a new class of 3-(1 ,2 -dihydroxyeth-1 -yl)-1-phenylpyrazolo [3,4-b] quinoxaline series "C-nucleosides" as antiviral agents Quinoxaline derivatives as new inhibitors of coxsackievirus B5 Synthesis and evaluation of quinoxaline derivatives as potential influenza NS1A protein inhibitors Quinoline tricyclic derivatives. Design, synthesis and evaluation of the antiviral activity of three new classes of RNA-dependent RNA polymerase inhibitors Synthesis, cytotoxicity and antiviral evaluation of new series of imidazo[4,5-g]quinoline and pyrido[2,3-g]quinoxalinone derivatives MK-5172, a selective inhibitor of hepatitis C virus NS3/4a protease with broad activity across genotypes and resistant variants Unraveling the structural basis of grazoprevir potency against clinically relevant substitutions in hepatitis C virus NS3/4A protease from genotype 1a Quinoxaline-Based Linear HCV NS3/4A Protease Inhibitors Exhibit Potent Activity against Drug Resistant Variants Hepatitis C Virus NS3/4A Protease Inhibitors Incorporating Flexible P2 Quinoxalines Target Drug Resistant Viral Variants Synthesis and biological activity of 7H-benzo[4,5]indolo[2,3-b]-quinoxaline derivatives Synthesis, cytotoxicity, antiviral activity and interferon inducing ability of 6-(2-aminoethyl)-6H-indolo[2,3-b]quinoxalines Design, synthesis and biological evaluation of quinoxaline compounds as anti-HIV agents targeting reverse transcriptase enzyme Design, synthesis and anti-HIV activity of novel quinoxaline derivatives Design, synthesis and anti-HIV-1 evaluation of hydrazide-based peptidomimetics as selective gelatinase inhibitors González-Scarano, F. 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