key: cord-0829166-rrzvlktb
authors: Krepel, Stacey A; Wang, Ji Ming
title: Chemotactic Ligands that Activate G-Protein-Coupled Formylpeptide Receptors
date: 2019-07-12
journal: Int J Mol Sci
DOI: 10.3390/ijms20143426
sha: 9c85321b0b17004a81cb40cc67c05d8939d03f12
doc_id: 829166
cord_uid: rrzvlktb

Leukocyte infiltration is a hallmark of inflammatory responses. This process depends on the bacterial and host tissue-derived chemotactic factors interacting with G-protein-coupled seven-transmembrane receptors (GPCRs) expressed on the cell surface. Formylpeptide receptors (FPRs in human and Fprs in mice) belong to the family of chemoattractant GPCRs that are critical mediators of myeloid cell trafficking in microbial infection, inflammation, immune responses and cancer progression. Both murine Fprs and human FPRs participate in many patho-physiological processes due to their expression on a variety of cell types in addition to myeloid cells. FPR contribution to numerous pathologies is in part due to its capacity to interact with a plethora of structurally diverse chemotactic ligands. One of the murine Fpr members, Fpr2, and its endogenous agonist peptide, Cathelicidin-related antimicrobial peptide (CRAMP), control normal mouse colon epithelial growth, repair and protection against inflammation-associated tumorigenesis. Recent developments in FPR (Fpr) and ligand studies have greatly expanded the scope of these receptors and ligands in host homeostasis and disease conditions, therefore helping to establish these molecules as potential targets for therapeutic intervention.

Formylpeptide receptors (FPR, Fpr for expression in mice) are G-protein-coupled receptors and were incidentally the first GPCRs to be identified in neutrophils [1] . Though initially cloned from neutrophils, FPRs have since been identified in macrophages, endothelial cells, intestinal epithelial cells, fibroblasts, and others [2] [3] [4] [5] . Humans possess three different forms of FPRs: FPR1, FPR2, and FPR3. FPR1 was the first named of the receptors, and it was initially discovered as the receptor for the formylated bacterial product formyl-methionine-leucyl-phenylalanine (fMLF), the name of which gave rise to the naming of the receptor in question [6] . FPR1 is most highly expressed in cells in the bone marrow and immune system, though it has some expression notable in cells of the lungs, brain, and gastrointestinal (GI) tract, among others [7, 8] . FPR2 was the second discovered of these receptors, but it tends to be the more ubiquitously expressed of the two. The expression is primarily in cells of the bone marrow, immune system, GI tract, female organ tissues, and endocrine glands, though there are also some lower levels of expression in cells of the brain, liver, gallbladder, and pancreas [8, 9] . There is little known about the biological significance of FPR3, and very little research has been done to elucidate its role. This receptor is mainly expressed in monocytes and dendritic cells but not neutrophils, and it resides in intracellular vesicles rather than on the cell surface like its counterpart receptors. FPR3, contrary to the other FPR variants, also has only one known endogenous peptide agonist [10, 11] .

The prototypic FPR ligand is fMLF, which was the first classified FPR agonist and also represents the shortest sequence to elicit a potent receptor response. While Fpr2 is generally considered the more promiscuous FPR, fMLF preferentially activates FPR1 with high affinity [29] . It has been shown that non-formylated bacterial peptides are much less potent than their formylated counterparts; a suggested reason for this is due to the use of formyl-methionine as a Gram-negative start codon, therefore marking a protein as pathogenic from the perspective of the host immune system [30] . There are many derivatives of fMLF which elicit FPR responses, many of which preferentially activate FPR2 rather than FPR1. Such derivatives include the peptide sequences fMLFII, fMLFIK, fMLFK, and fMLFW, among others [31] . Liu et al. [32] summarized many other formylated peptides that elicit responses through both FPR1 and FPR2, including f-MIFL, f-MIVIL, f-MIGWII, and f-MFEDAVAWF. Dozens of similar peptides have been isolated from equally numerous bacterial genera including Streptococcus, Haemophilus, Salmonella, Hydrogenobacter, Listeria, Neisseria, Staphylococcus, and others [27] . PSMα is another formylated peptide that has shown great efficacy in FPR activation. Thus far, the phenol-soluble modulins that have demonstrated a capacity for FPR activation include β2, α1, and α2, all of which are virulence factors isolated from Staphylococcus aureus. All three peptides activate FPR2, though the structural basis for activation remains unknown [33] .

It is interesting to note that mammalian cell mitochondria, well-known for being bacterial in origin, also contain peptides that elicit FPR-mediated responses. However, while bacterial formylated peptides are considered pathogen-associated molecular patterns (PAMPs), the mitochondrial peptides are generally associated with cellular damage and are thus considered damage-associated molecular patterns (DAMPs) that elicit an inflammatory response [34] . These peptides, some of which include MMYALF, MFADRW, and Nle-LF-Nle-YK, have been tied to constriction of airways in the lungs, as well as neutrophil accumulation and other proinflammatory responses [35, 36] . Likewise, lung diseases-including acute respiratory distress syndrome-have been found to have a higher presence of formylated mitochondrial peptides in bronchoalveolar lavage fluid, suggesting that lung inflammation is tightly tied to Fpr1 activation via these DAMPs [37] . Mitocryptide-2 (MCT-2) is another mitochondrial peptide. It is related to Cytochrome B of the electron transport chain and activates FPR2. Interestingly, while the N-terminal formylated methionine is entirely necessary for FPR2 activation by MCT-2, the carboxy-terminal residues are also important for receptor activation. Additionally, sequence analysis has revealed that the presence of the residues Thr7 and Ser8 can activate FPR2, though the peptides were most potent with Ile7 and Asn8 residues [38] .

Another important mitochondrial peptide is the nicotinamide adenine dinucleotide (NADH) reductase subunit 1 (ND-1), which elicits a strong inflammatory response via FPR1 [39] . Bufe and Zufall [40] have successfully created a model that accurately predicts known mitochondrial peptide agonists for FPRs. Therefore, there is a strong likelihood for the discovery of many new mitochondrial, host-derived FPR agonist peptides. Another DAMP that elicits FPR-mediated responses is Mitochondrial Transcription Factor A (Tfam). While this necrosis-associated peptide has been previously shown to activate FPRs, activation does not appear to play a crucial role in the inflammatory responses of monocytic microglia in the brain; this may be due to lower FPR expression in this cell type [41, 42] . The formylated peptide ligands for FPRs (Fprs) are listed in Table 1 , and the mitochondrial peptides are listed in Table 2 . Table 3 lists the microbe-derived FPR (Fprs) ligands. While formylated peptides first drew the attention of the scientific community to FPRs (Fprs), there are many other bacterial/viral peptides that are not necessarily formylated but which nevertheless elicit receptor responses. Although the majority of formylated microbial peptides preferentially activate FPR1, the preferred receptor for non-formylated peptides is FPR2 [26] . A large percentage of these non-formylated microbe-derived peptides are viral, and many of them are derived from the Human Immunodeficiency Virus (HIV) envelope proteins, including gp41 T20/DP178, gp41 T21/DP107, gp120 V3 loop, gp41 N36, gp120 F, and gp41 MAT-1 [44] [45] [46] . Despite the potential importance of FPRs in HIV research, very little work has been done to further explore this connection. However, Li et al. [47] demonstrated that persistent FPR activation desensitized host CCR5 and CXCR4 co-receptors to HIV proteins, thus reducing viral entry and subsequent replication. Still other viruses, including Hepatitis C Virus, HKU-1 coronavirus, and Herpes Simplex Virus, produce chemotactic ligands C5a, N-formyl HKU-1 coronavirus peptide, and gG-2p20, respectively, for FPR1 or FPR2 activation [48] [49] [50] . There is, however, some argument as to the efficacy of the Herpes Simplex viral peptide as an FPR agonist, as the overlapping sequence gG-2p19 was unable to definitively demonstrate that FPR activation played a significant role in the NK response to this virus [51] . Mills [49] used the sequence homology of T20/DP178 to further determine that the OC43 Coronavirus, 229E Coronavirus, NL36 Coronavirus, and even the Ebola Spike Protein were all peptides with aromatic-rich domains that elicited FPR-dependent cell activation. Interestingly, when examined from the context of the FPRs rather than the ligands, it was found that domain variability in the receptors determined ligand binding and subsequent cellular responses. This led to the conclusion that the variability of receptors among individuals might predispose or protect against certain viral infections, the susceptibility of which may be determined by receptor activation. In terms of non-viral and non-formylated microbe-derived peptides, there are few FPR agonists. Certain peptides from different strains of Enterococcus faecium have demonstrated FPR activation properties, though the ligand activity is not entirely predictable based on structure. Interestingly, E. faecium strains that are resistant to vancomycin contain potent FPR2 agonists, suggesting a potential role for FPR2 in antibiotic-resistant infections [52] .

There are many different host-derived ligands that elicit strong FPR responses, though they have different biological implications. Misfolded proteins implicated in a variety of pathologies are one class of host-derived proteins eliciting FPR activation. Amyloid β-42 (Aβ-42), a peptide fragment well-documented in Alzheimer's Disease (AD), interacts with FPR2. Additionally, part of FPR2's role includes interaction with the Macrophage Receptor with Collagenous Structure (MARCO) scavenger receptor, which is responsible for reducing inflammation and alleviating inflammation-associated symptoms [60] . Interestingly, the symptoms that are viewed as a hallmark of AD are due to this same ligand binding an entirely different group of receptors which do not internalize them, therefore leading to an increased inflammatory pathology [61] . The prion peptide fragment, PrP 106-126 also interacts with FPR2 on astrocytes and microglia, and the internalization of this peptide is detrimental to the host and contributes to disease progression [62] . Another neuropeptide that activates FPR2-though other studies claim it also activates FPR1-is the pituitary adenylate cyclase-activating polypeptide 27 (PACAP27), which has been shown to induce migration and Ca 2+ mobilization, as well as upregulation of CD11b in neutrophils [63, 64] . Another FPR agonist, the Vasoactive Intestinal Peptide (VIP), activates monocytes via FPR2 and may initiate an inflammation-resolving process [64, 65] .

Other host-derived FPR ligands are CKβ8-1 and the SHAAGtide sequence, as well as various uPAR domains from the Urokinase-Type 1 Plasminogen Activator Receptor (uPAR). CKβ8-1 is also known as the CCL23 chemokine, and it acts as an agonist for FPR2, along with its truncated N-terminal peptide called the SHAAGtide sequence that activates a chemokine GPCR [66, 67] . It has been demonstrated that several uPAR peptides elicit FPR responses, including uPAR [88] [89] [90] [91] [92] , uPAR [84] [85] [86] [87] [88] [89] [90] [91] [92] [93] [94] [95] , D2D3, and the SRSRYp sequence [68] [69] [70] . These sequences may foster the transition between fibroblasts to myofibroblasts, therefore increasing the pathology of fibrosis via FPR2 activation [68] . Another host-derived peptide, F2L, is derived from the N-terminus of the heme-binding protein, HEPB1. As the sole agonist specific for FPR3, F2L activates macrophages and possibly dendritic cells (DCs) as well [71] . However, other studies have demonstrated that FPR2 in neutrophils also exhibits a moderate affinity for F2L, though in this scenario F2L appears to have an inhibitory rather than a stimulatory effect [72, 73] . A newer FPR ligand is Family with Sequence Similarity 3 (Member D), or FAM3D. This chemokine-like peptide is most highly expressed in the GI tract, though it is also expressed in cells of the immune system [8, 74] . FAM3D has demonstrated a high affinity for both FPR1 and FPR2 and has been implicated in playing an important role in both inflammation and GI homeostasis via FPR activation [75] . Additional studies have found that FAM3D may also be involved in the beneficial role of glucagon secretion in Type 2 diabetes, as well as the detrimental development of abdominal aortic aneurysms [76, 77] .

In addition to endogenous peptides associated with cell-surface proteins and functional units, there are many ligands that are secreted by cells in response to tissue damage. Annexin-1 (AnxA1), also called Lipocortin-1, is an anti-inflammatory protein which is upregulated as a result of the stress responses of multiple host systems [78] . Some studies demonstrate AnxA1 to be an FPR1 agonist, while others show it as an FPR2 agonist; hence, it likely activates both. One study demonstrated its role in the attenuation of rheumatoid arthritis symptoms by decreasing fibroblast-like synoviocyte proliferation via FPR2 [79] . However, another study showed that AnxA1 initiated autocrine signaling in breast cancer via FPR1 and led to an increase in tumor growth and metastasis [80] . Additionally, the absence of AnxA1 has been tied to increased disease severity in both rheumatoid arthritis and obstructive pulmonary disease, leading to the hypothesis that treatment with exogenous AnxA1 may help reduce symptoms associated with different inflammatory pathologies [81, 82] . In addition to AnxA1, multiple derivatives of the parent protein, including Ac 1-25 , Ac 2-26 , and Ac 9-25 , activate FPR2 [83] [84] [85] . These peptides have protective effects in ischemia-induced lung injury and atherosclerosis [84, 86] . As with its pro-survival property, AnxA1 acts as a double-edged sword: secretion of either AnxA1 or Ac 2-26 by tumor-associated fibroblasts induces the acquisition of stem-like features in prostate cancer cells, thus leading to a worse prognosis [87] .

Serum-amyloid alpha (SAA) is an endogenous FPR2 agonist secreted by liver or macrophages in response to inflammatory stress and, more notably, tissue damage. In endothelial cells, SAA enhances the expression and activity of Tissue Factor-a protein necessary for clotting and wound repair-while additionally inhibiting the activity of Tissue Factor Pathway Inhibitor. Both functions were demonstrated to be the result of FPR2 activation [88] . SAA, via FPR2 activation, additionally increases the production of the wound-healing chemokine, CCL2, by vascular endothelial cells [89] . More recent studies have demonstrated the role of an SAA-FPR2 axis in neovascularization in the cornea as well [90, 91] .

Human LL-37 is an antimicrobial peptide that induces Cxcl13 and Tnfsf13b transcription, as well as B cell activation and proliferation via FPR2. It also contributes to the maintenance of B-cell germinal centers in Peyer's Patches of the gut [92] . LL-37 also promotes the growth of both colorectal and ovarian cancer cells [93, 94] . The murine homologue of LL-37, Cathelicidin-related antimicrobial peptide (CRAMP), is similarly an Fpr2 agonist and has been shown to promote atherosclerosis and DC maturation [95, 96] . CRAMP also plays a pivotal role in maintaining the homeostasis of the colon mucosa and microbiota balance, demonstrating its potential as a therapeutic molecule [97] . The list of host-derived FPR (Fpr) ligands is shown in Table 4 . Vasoactive intestinal protein FPR2 [65] Lipoxin-A4/Apsirin-triggered lipoxins Fatty Acid FPR2 [103] 

By far the most extensive category of FPR ligands includes the synthetic and small-molecule ligands, of which there are over 40 currently known, as shown in Table 5 . W-peptides are among the better-known synthetic peptides acting as FPR agonists, and they include the sequences WKYMVm-NH 2 , WKYMVM-NH 2 , as well as many derivatives. Bufe et al. [27] demonstrated that both FPR1 and FPR2 mediate Ca 2+ mobilization responses in leukocytes to more than 20 different combinations and derivatives of the W-peptide. A breakdown of the data suggests that certain residues in the peptide sequence are more important than others: C3 tyrosine, C4 methionine, and C6 D-methionine are all required for ligand activity, as is the carboxy-terminal NH 2 . Peptides may additionally be shortened on the N-terminus by two amino acids or elongated by three amino acids before FPR activation capacity is severely diminished. While the applications of WKYMV-sequences have been little explored, one recent study showed that the activation of FPR2 by WKYMVm may enhance the homing of endothelial cells, thus improving tissue healing, especially in ischemic neovasculogenesis in injured limbs [3] . Interestingly, another study showed that WKYMVm was capable of desensitizing HIV coreceptors CXCR4 and CCR5, therefore decreasing the entry of HIV-1 into macrophages and CD4+ T cells [47] .

M-peptides are another subclass of synthetic/peptide library isolates and include MMK-1 and MMWLL. MMK-1, an FPR2 agonist, is by far the more commonly used peptide, and studies have shown that it may be useful as an anti-anxiety drug, as well as a drug to counteract hair loss from chemotherapy [98, 104] . However, there has been some concern about its use in certain drug regimens, as it may amplify the response of monocytes to SIO 2 -coated nanoparticles, making it an important player in calculating the proper dosing when using such nanoparticles [105] . MMWLL is another M-peptide specific for FPR1. It is not classically a formylated peptide, though the addition of a formylated methionine induces a more potent FPR1 response than even the classic prototypic fMLF [106] . A much newer class of synthetic peptides are the FPR1-agonistic AApeptides, based on the general structure of N-acylated-N-aminoethyl amino acid residues. There are three different AApeptide subgroups called the α-peptides, α-AApeptides, and γ-AApeptide, all of which have different R-groups at the designated α or γ position. Most of these derivatives of AApeptides induce Ca 2+ mobilization in rat basophil leukemia (RBL) cells transfected with human FPR1, though the γ-AApeptide Compound 7 at 10 µM elicited a more potent cell response than fMLF at the same concentration, making it a reasonably high-affinity ligand for FPR1 at this concentration, though not at lower concentrations [107] . See Table 5 for all synthetic peptides. Some newer, non-peptide synthetic compounds being studied are various derivatives of ureidopropanamide molecules. They have demonstrated a capacity for protection against LPS-induced microglial death via an FPR2-dependent pathway, and pre-treatment with concentrations as low as 1 µM showed the protective effects [24] . Thus, these ligands show promising potential as treatment for diseases associated with inflammation in the Central Nervous System (CNS). Another pro-resolving synthetic ligand is the quinazolinone derivative Quin-C1. It is an Fpr2 agonist and has been shown to be effective at reducing inflammatory cytokines and clearing neutrophils and lymphocytes in murine models of lung injury [114] . Schepetkin et al. [115] demonstrated that three different synthetic molecules are agonists for both FPR1 and FPR2. Two of these are bombesin-related BB 1 /BB 2 antagonists called PD168368 and PD176252, and they induce Ca 2+ flux as well as neutrophil degranulation with EC50 values in the nanomolar range, thus making them potent FPR agonists. The third agonist is the Cholecystokinin-1 receptor agonist A-71623, which exhibits FPR1 and FPR2 agonism, though with a much higher EC50. In structural studies, these ligands were cross-reactive with FPR1/2 and possessed both Trp and N-phenylurea moieties. This led to the hypothesis that the combination of moieties greatly increases the chance that an agonist will activate both receptors.

Kirpotina et al. [116] screened over 6000 compounds and isolated nearly 30 different FPR1 and/or FPR2 agonists, all of which are derivatives of acetohydrazide, 2-(N-piperazinyl)acetamide, N'-phenylurea, and benzimidazole. The acetohydrazide derivatives (compounds AG-07/7, AG-09/92, AG-09/96, AG-09/101, and AG-09/102) and N-phenylurea derivatives (AG-09/3, AG-09/4, AG-09/73 through AG-09/77, and AG-09/82) are all FPR2-specific, though the acetohydrazide compounds tend to have lower efficacies on average. Also, the benzimidazole derivatives are either FPR1-specific (AG-09/1, AG-09/2, AG-09/13, AG-09/18, AG-09/19, and AG-09/21) or are agonists for both FPR1 and FPR2 (AG-09/16, AG-09/17, AG-09/20, and AG-09/22 through AG-09/24); no FPR2-specific benzimidazole derivatives have yet been identified. Pyridazines are another class of non-peptide, synthetic molecules which can have many different derivatives. Currently, only two compounds have been identified as potent mixed FPR1 and FPR2 agonists with an EC50 of around 2 µM each. These two compounds are referred to as compounds 8b and 8c with the Pyridazin-3(2H)-one structure. Additionally, they have R substitutions of SCH3 and OCH3, as well as R1 substitutions of I and SCH3, respectively. Both R and R1 substitutions are on substituted benzene rings [117] . (See Table 6 for the list of synthetic/small molecule non-peptide agonists). 

As the search for disease treatments continues, many investigators have turned to developing compounds isolated from various plants and animals for potential therapeutic uses. Some of these new compounds have been shown to activate human or murine FPRs. The first of these is a series of compounds isolated from the centipede Scolopendra subspinipes mutilans, which has classically been used in Oriental medicine and is now being studied for therapeutic potential [126] . New studies show that compounds Scolopendrasin III and V both cause human neutrophil migration via FPR1, while Scolopendrasin IX seems to work through FPR2 to promote neutrophil chemotaxis. The two former compounds have not yet been studied for effectiveness against particular pathologies, though Compound IX has traditionally been an effective treatment for rheumatoid arthritis in Oriental medicine. New evidence confirms this activity, citing the activation of FPR2 as the mechanism [9, 127] . Temporins are another class of FPR agonists and consist of antimicrobial peptides isolated from the Rana Temoraria frogs. Temporin A and Rana-6 are two such peptides, and both activate FPR2 to promotion leukocyte migration. There are also two distinct synthetic peptides, I4S10-C and I4G10-C, that are modeled after temporins and activate FPR2 [128] .

Rubimetide is a peptide (Met-Arg-Trp) isolated from the digest of Rubisco in spinach. While it has been studied for some time, it has just recently been classified as an FPR2 agonist and has further demonstrated an ability to produce anxiolytic-like effects, thus alleviating some symptoms associated with anxiety [98] . The same investigators also isolated soymetide from the α' subunit of β-conglycinin from soybeans and then demonstrated its activity as an FPR1 agonist [129] . Furthermore, the antimicrobial peptides Piscidin-1 and -3, which were isolated from fish, have been shown to induce myeloid cell chemotaxis via both FPR1 and FPR2. As a testament to the harms of water pollution by metals, this study also demonstrated that conjugation of Cu 2+ with either of these compounds reduces the chemotactic activity of mammalian neutrophils [130] (See Table 7 for the list of peptides from other non-human sources). 

FPRs are a class of seven-transmembrane, G-protein-coupled receptors (GPCRs) that interact with a remarkably diverse range of ligands. As demonstrated, these ligands may originate from pathogens, the host, the synthetic peptide or compound library, or even non-host multicellular organisms. With such diverse agonist binding capacity, it is not surprising that FPRs may be either detrimental or beneficial in different pathophysiological conditions. Though the majority of these agonists have been known for more than a decade, newer studies are finding novel roles for these ligands in treatments for conditions ranging from anxiety and mental health disorders to arthritis and wounds. The field of FPR agonist studies has demonstrated the potential of these molecules to have therapeutic mechanisms useful for medicine. In addition to the vast number of agonists summarized here, there are also extensive lists of antagonistic ligands that may also provide protective mechanisms in various diseases [23, 44] . Thus, further exploration of FPRs and ligands as therapeutic targets would be highly beneficial to diseases including cancer, sceptic shock, arthritis, and many other inflammatory pathologies. 

The role of formylated peptides and formyl peptide receptor 1 in governing neutrophil function during acute inflammation

Formyl-peptide receptors revisited

WKYMVm-induced activation of formyl peptide receptor 2 stimulates ischemic neovasculogenesis by promoting homing of endothelial colony-forming cells

Expression and functional role of formyl peptide receptor in human bone marrow-derived mesenchymal stem cells

Expression and function of formyl peptide receptors on human fibroblast cells

Synthesis and use of a novel N-formyl peptide derivative to isolate a human N-formyl peptide receptor cDNA

V18.1.proteinatlas.org. FPR1 Tissue Expression

Tissue-based map of the human proteome

V18.1.proteinatlas.org. FPR2 Tissue Expression

N-formyl peptide receptor 3 (FPR3) departs from the homologous FPR2/ALD receptor with regard to the major processes governing chemoattractant receptor regulation, expression at the cell surface, and phosphorylation

Functional expression of formyl peptide receptor family in human NK cells

G-protein-coupled formyl peptide receptors play a dual role in neutrophil chemotaxis and bacterial phagocytosis

FAM19A4 is a novel cytokine ligand of formyl peptide receptor 1 (FPR1) and is able to promote the migration and phagocytosis of macrophages

Formylpeptide receptors mediate rapid neutrophil mobilization to accelerate wound healing

Formylpeptide receptor-2 contributes to colonic epithelial homeostasis, inflammation, and tumorigenesis

The G-protein coupled chemoattractant receptor FPR2 promotes malignant phenotype of human colon cancer cells

The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis

The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis

Designer cells programming quorum-sensing interference with microbes

Nonredundant protective properties of FPR2/ALX in polymicrobial murine sepsis

Formyl peptide receptor as a novel therapeutic target for anxiety-related disorders

Formyl peptide receptor-1 activation exerts a critical role for the dynamic plasticity of arteries via actin polymerization

The formyl peptide receptors: Diversity of ligands and mechanism for recognition

Novel ureidopropanamide based N-formyl peptide receptor 2 (FPR2) agonists with potential application for central nervous system disorders characterized by neuroinflammation

Ligand-specific conformational change of the G-protein-coupled receptor ALX/FPR2 determines proresolving functional responses

Formylated MHC Class Ib binding peptides activate both human and mouse neutrophils primarily through formyl peptide receptor 1

Recognition of bacterial signal peptides by mammalian formyl peptide receptors: A new mechanism for sensing pathogens

Peptide length and folding state govern the capacity of staphylococcal B-type phenol-soluble modulins to activate human formyl-peptide receptors 1 or 2

Purification and identification of formyl-methionyl-leucyl-phenylalanine as the major peptide neutrophil chemotactic factor produced by Escherichia coli

Formyl-methionine as a degradation signal at the N-termini of bacterial proteins. Microb

Structural determinants for the interaction of formyl peptide receptor 2 with peptide ligands

Chapter 91: FPR Ligands

Solution structures of phenol-soluble modulins a1, a2, B2, virulence factors from Staphylococcus aureus

Circulating mitochondrial DAMPs cause inflammatory responses to injury

Mitochondrial N-formyl peptides cause airway contraction and lung neutrophil infiltration via formyl peptide receptor activation

Mitochondrial peptides cause proinflammatory responses in the alveolar epithelium via FPR-1, MAPKs, and AKT: A potential mechanism involved in acute lung injury

Novel role for endogenous mitochondrial formylated peptide-driven formyl peptide receptor 1 signaling in acute respiratory distress syndrome

Identification of residues critical for FPR2 activation by the cryptic peptide mitocryptide-2 originating from the mitochondrial DNA-encoded cytochrome b

Peptides from the amino-terminus of mouse mitochondrially encoded NADH dehydrogenase subunit 1 are potent chemoattractants

The sensing of bacteria: Emergin principles for the detection of signal sequences by formyl peptide receptors

Mitochondrial transcription factor A (Tfam) is a pro-inflammatory extracellular signaling molecule recognized by brain microglia

DAMPs in Cardiovascular Disease Working Group. Mitochondrial damage-associated molecular patterns and vascular function

FITC conjugation markedly enhances hepatic clearance of N-formyl peptides

Chemotactic Peptide Ligands for Formylpeptide Receptors Influencing Inflammation

Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes

The HIV-1 gp41 ectodomain is cleaved by matriptase to produce a chemotactic peptide that acts through FPR2

The synthetic peptide WKYMVm attenuates the function of the chemokine receptors CCR5 and CXCR4 through activation of formyl peptide receptor-like 1

C5a), an amphipathic a-helical peptide of hepatitis Virus C, is an activator of N-formyl receptor in human phagocytes

Peptides derived from HIV-1, HIV-2, Ebola virus, SARS coronavirus and coronavirus 229E exhibit high affinity binding to the formyl peptide receptor

A proinflammatory peptide from herpes simplex virus type 2 glycoprotein G affects neutrophil, monocyte, and NK cell functions

A monocyte-specific peptide from herpes simplex virus type 2 glycoprotein G activates the NADPH-oxidase but not chemotaxis through a G-protein-coupled receptor distinct from the members of the formyl peptide receptor family

Enterococcus faecium stimulates human neutrophils via the formyl-peptide receptor 2

A proinflammatory peptide from Helicobacter pylori activates monocytes to induce lymphocyte dysfuntion and apoptosis

T21/DP107, a synthetic leucine zipper-like domain of the HIV-1 envelope gp41, attracts and activates human phagocytes by using G-protein-coupled formyl peptide receptors

Activation of the chemotactic peptide receptor FPRL1 in monocytes phosphorylates the chemokine receptor CCR5 and attenuates cell responses to selected chemokines

N36, a synthetic N-terminal heptad repeat domain of the HIV-1 envelope protein gp41, is an activator of human phagocytes

A synthetic peptide derived from human immunodeficiency virus type 1 gp120 downregulates the expression and function of chemokine receptors CCR5 and CXCR4 in monocytes by activating the 7-transmembrane G-protein-coupled receptor FPRL1/LXA4R

The neurotoxic prion peptide fragment PrP(106-126) is a chemotactic agonist for the G protein-coupled receptor formyl peptide receptor-like1

Formyl peptide receptor 2 plays a deleterious role during influenza A virus infection

Functional and physical interactions between formyl-peptide-receptors and the scavenger MARCO and their involvement in amyloid beta 1-42-induced signal transduction in glial cells

Amyloid-B receptors: The good, the bad, and the prion protein

Internalization of PrP(106-126) by the formyl-peptide-receptorlike-1 in glial cells

Pituitary adenylate cyclase-activating polypeptide 27 is a functional ligand for formyl peptide receptor-like 1

Vasoactive intestinal peptide dampens formyl-peptide-induced ROS production and inflammation by targeting a MAPK-p47 phox phosphorylation pathway in monocytes

VIP modulates the ALX/FPR2 receptor axis toward inflammation resolution in a mouse model of bacterial keratitis

A truncated form of CKβ8-1 is a potent agonist for human formyl peptide-receptor-like 1 receptor

International union of basic and clinical pharmacology. LXXIII. Nomenclature for the formyl peptide receptor family

Upregulation of the N-formyl peptide receptors in Scleroderma Fibroblasts fosters the switch to myofibroblasts

Urokinase induces basophil chemotaxis through a urokinase receptor epitope that is an endogenous ligand for formyl peptide receptor-like 1 and -like 2

Cross-talk between fMLP and vitronectin receptors triggered by urokinase receptor-derived SRSRY peptide

Processing of HEBP1 by chathepsin D gives rise to F2L, the agonist of formyl peptide Receptor 3

F2L, a peptide derived from heme-binding protein, chemoattracts mouse neutrophils by specifically activating Fpr2, the low-affinity N-formylpeptide receptor

F2L, a peptide derived from heme-binding protein, inhibits LL-37-induced cell proliferation and tube formation in human umbilical vein endothelial cells

V18.1.proteinatlas.org. FAM3D Tissue Expression

Identification of FAM3D as a new endogenous chemotaxis agonist for the formyl peptide receptors

FAM3D inhibits glucagon secretion via MKP1-dependent suppression of ERK1/2 signaling

Deficiency of FAM3D (Family with sequence similarity 3, member D), a novel chemokine, attenuates neutrophil recruitment and ameliorates abdominal aortic aneurysm development

Stress: Neuroendocrinology and Neurobiology: Handbook of Stress Series

Formyl peptide receptor activation inhibits the expansion of effector T cells and synovial fibroblasts and attenuates joint injury in models of rheumatoid arthritis

Inhibition of the AnxA1/FPR1 autocrine axis reduces MDA-MB-231 breast cancer cell growth and aggressiveness in vitro and in vivo

A formyl peptide receptor agonist suppresses inflammation and bone damage in arthritis

Defective formyl peptide receptor 2/3 and annexin A1 expression associated with M2a polarization of blood immune cells in patients with chronic obstructive pulmonary disease

Transcriptional profiling of human monocytes reveals complex changes in the expression pattern of inflammation-related genes in response to the annexin A1-derived peptide Ac1-25

Ac2-26, an Annexin A1 peptide, attenuates ischemia-repurfusion-induced acute lung injury

Neutrophil NADPH-oxidase activation by an annexin A1 peptide is transduced by the formyl peptide receptor (FPR), whereas an inhibitory signal is generated independently of the FPR family receptors

Targeted nanoparticles containing the proresolving peptide Ac2-26 protect against advanced atherosclerosis in hypercholesterolemic mice

CAF-secreted annexin A1 induces prostate cancer cells to gain stem cell-like features

Impact of serum amyloid A on tissue factor and tissue factor pathway inhibitor expression and activity in endothelial cells

A pertussis toxin sensitive G-protein-independent pathway is involved in serum amyloid A-induced formyl peptide receptor 2-mediated CCL2 production

Serum Amyloid A1 is an epithelial prorestitutive factor

Serum amyloid A and pairing formyl peptide receptor 2 are expressed in corneas and involved in inflammation-mediated neovascularization

Cutting edge: LL-37-mediated formyl peptide receptor-2 signaling in follicular dendritic cells contributes to B cell activation in Peyer's Patch germinal centers

Antimicrobial peptide LL-37 in macrophages promotes colorectal cancer growth

Roles and mechanisms of human cathelicidin LL-37 in cancer

Lack of neutrophil-derived CRAMP reduces atherosclerosis in mice

The formylpeptide receptor 2 (Fpr2) and its endogenous ligand cathelin-related antimicrobial peptide (CRAMP) promote dendritic cell maturation

The antimicrobial peptide CRAMP is essential for colon homeostasis by maintaining microbiota balance

Rubimetide, humanin, and MMK1 exert anxiolytic-like activities via the formyl peptide receptor 2 in mice followed by the successive activation of DP 1 , A 2A , and GABA A receptors

Antiflammin-2 activates the human formyl-peptide receptor like 1

Identification of neutrophil granule protein cathepsin G as a novel chemotactic agonist for the G protein-coupled formyl peptide receptor

FPR2/ALXR agonists and the resolution of inflammation

Aspirin triggered resolvin D1 reduces inflammation and restores saliva secretion in a Sjogrens syndrome mouse model

Lipoxin A4 reduces inflammation through formyl peptide receptor 2/p38MAPK signaling pathway in subarachnoid hermorrhage rats

Orally administered FPRL1 receptor agonist peptide MMK-1 inhibits etoposide-induced alopecia by a mechanism different from intraperitoneally administered MMK-1. Peptides

Formyl-peptide receptor agonists and amorphous SiO2-NPs synergistically and selectively increase the inflammatory responses of human monocytes and PMNs

Tethered ligand library for discovery of peptide agonists

Design, synthesis and characterization of fMLF-mimicking AApeptides

A novel peptide agonist of Formyl-peptide receptor-like 1 (ALX) displays anti-inflammatory and cardioprotective effects

A pepducin designed to modulate P2Y2R function interacts with FPR2 in human neutrophils and transfers ATP to an NADPH-oxidase-activating ligand through a receptor cross-talk mechanism

Formyl peptide receptors in mice and men: Similarities and differences in recognition of conventional ligands and modulating lipopeptides

Identification of novel peptides that stimulate human neutrophils

The lipidated peptidomimetic Lau-((S)-Aoc)-(Lys-Bnphe)6-NH2 is a novel formyl peptide receptor 2 agonist that activates both human and mouse neutrophil NADPH oxidase

Apolipoproteins and apolipoprotein mimetic peptides modulate phagocyte trafficking through chemotactic activity

Characterization of Quin-C1 for its anti-inflammatory property in a mouse model of bleomycin-induced lung injury

Gastrin-releasing peptide-neuromedin B receptor antagonist PD176252, PD168368, and related analogs are potent agonists of human formyl-peptide receptors

Identification of novel small-molecule agonists for human formyl peptide receptors and pharmacophore models of their recognition

Synthesis and pharmacological evaluation of new pyridazine-based thioderivatives as formyl peptide receptor (FPR) agonists

New 'chemical probes' to examine the role of the hFPRL1 (or ALXR) receptor in inflammation

Synthesis, enantioresolution, and activity profile of chiral 6-methyl-2,4-disubstituted pyridazine-3(2H)-ones as potent N-formyl peptide receptor agonists

Selective agonists and antagonists of formylpeptide receptors: Duplex flow cytometry and mixture-based positional scanning libraries

Novel 3-(1H-Indol-3-yl)-2-[3-(4-nitrophenyl)ureido]propanamides as selective agonists of human formyl-peptide receptor 2

The anti-infective peptide, innate defense-regulator peptide, stimulates neutrophil chemotaxis via a formyl peptide receptor

Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment

Small-molecule-biased formyl peptide receptor agonist compound 17b protects against myocardial ischaemia-reperfusion injury in mice

6-methyl-2,4-disubstituted pyridazin-3(2H)-ones: A novel class of small-molecule agonists for formyl peptide receptors

Centipede venoms and their components: Resources for potential therapeutic applications

A novel antimicrobial peptide acting via formyl peptide receptor 2 shows therapeutic effects against rheumatoid arthritis

Temporin A and related frog antimicrobial peptides use formyl peptide receptor-like 1 as a receptor to chemoattract phagocytes

Soymetide, an immunostimulating peptide derived from soybean β-conglycinin, is an fMLP agonist

Copper regulates the interactions of antimicrobial piscidin peptides from fish mast cells with formyl peptide receptors and heparin

Promotion of formyl peptide receptor 1-mediated neutrophil chemotactic migration by antimicrobial peptides isolated from the centipede Scolopendra subspinipes mutilans

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license

The authors thank Cheri Rhoderick for secretarial assistance.

The authors declare no conflict of interest.