key: cord-0830367-vlnmmd93
authors: Akhter, Sohail; Berchel, Mathieu; Jaffrès, Paul-Alain; Midoux, Patrick; Pichon, Chantal
title: mRNA Lipoplexes with Cationic and Ionizable α-Amino-lipophosphonates: Membrane Fusion, Transfection, mRNA Translation and Conformation
date: 2022-03-07
journal: Pharmaceutics
DOI: 10.3390/pharmaceutics14030581
sha: 47a020c6a9fd77e049abd05e70fd71a38804f723
doc_id: 830367
cord_uid: vlnmmd93

Cationic liposomes are attractive carriers for mRNA delivery. Here, mRNA lipoplexes (LX) were prepared with the cationic lipids α-aminolipophosphonate (3b) or imidazolium lipophosphoramidate (2) associated with various α-aminolipophosphonates co-lipids comprising protonable groups (imidazole or pyridine) and DOPE. Physicochemical parameters of liposomes and their membrane fusion activity were measured. LXs comprising either 3b- or 2- allowed transfection of ~25% and 40% of dendritic cells with low cytotoxicity, respectively; the efficiency increased up to 80% when 2 was combined with the imidazole-based co-lipid 1. The transfections were high with 3b/1, 3b/DOPE, 2/1 and 2/DOPE LXs. We observed that the transfection level was not well correlated with the acid-mediated membrane fusion activity of liposomes supposed to destabilize endosomes. The mRNA release from LXs and its translation capacity after release were studied for the most efficient LXs. The results showed that the more mRNA was condensed, the poorer the translation efficiency after release was. In contrast to DNA, circular dichroism performed on mRNA complexed with 2/DOPE revealed the presence of denatured mRNA in LXs explaining this lack of translation efficiency. This is an important parameter that should be stressed for the preparation of mRNA LXs with a conserved mRNA translation activity.

Today, the effectiveness of mRNA vaccines is demonstrated through the development and use of approved vaccines for the COVID-19 virus [1] [2] [3] [4] [5] [6] . This efficiency now makes it possible to consider using synthetic mRNAs for multiple therapeutic applications such as viral infections, cancer, gene therapy, cell reprogramming, and genome editing (CRISPR/Cas9) [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] . For these purposes, mRNA delivery systems are required to protect mRNA against nucleases and ultimately to deliver mRNA to the target cells in vivo, to release it intracellularly and to allow for its efficient translation. For vaccines, lipid nanoparticles (LNP) have demonstrated efficacy to protect synthetic mRNA or self-amplifying mRNA from RNAse degradation and increase their cellular uptake upon intramuscular injection. LNP formulations consisting of (i) one ionizable lipid or polymer containing tertiary amines featuring a pKa < 6.5; (ii) one amphoteric lipid such as 1,2-distearoyl-sn-glycero-3phosphocholine (DSPC) mimicking the lipid membrane in cells; (iii) cholesterol; and (iv) one polyethylene glycol (PEG)-lipid conjugate. Besides LNP, cationic liposomes remain attractive vectors for mRNA delivery. For all formulations, the cytosolic delivery of mRNA liposomes remain attractive vectors for mRNA delivery. For all formulations, the cytosolic delivery of mRNA is a crucial step. Lipoplexes (LX) are mostly prepared by the association of a cationic lipid and a co-lipid. Cationic lipids allow electrostatic interactions with nucleic acids while the co-lipids contribute to reduce cytotoxicity and induce the destabilization of the endosomal membrane that favors mRNA transfer in the cytosol [19] . DOPE (1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine) is the most common co-lipid used because it has the propensity to induce favorable supramolecular changes (from lamellar to inverted hexagonal supramolecular assemblies), thus promoting membrane fusion in a mild acid environment typical of the endosome lumen [20] [21] [22] [23] [24] . Likewise, lipids containing histidine or imidazole polar head group promote the endosomal escape of the nucleic acid through protonation of imidazole moiety in the acid lumen of endosomal vesicles by contributing to the proton-sponge phenomena and/or the membrane fusion effect [25] [26] [27] . Liposomes made with an equimolar ratio of the O,O-dioleyl-N-(3-N-(N-methylimidazolium iodide)propylene) phosphoramidate (2) and O,O-dioleyl-N-histamine phosphoramidate (1) were successfully used for in vitro DNA and RNA transfection with negligible cytoxicity [28, 29] and for in vivo transfection in association with cationic polymers ( Figure  1 ) [30] [31] [32] . Here, we evaluated mRNA transfection with cationic liposomes comprising either the lipid 2 or the α-amino-lipophosphonate featuring tertiary amine (3b) as cationic lipids associated with either DOPE or pH responsive α-amino-lipophosphonate lipids as co-lipids ( Figure 1 ) [33] . The membrane fusion activity of liposomes were measured at pH 7.4 and pH 5.5 and transfection efficiencies of lipoplexes with mRNA encoding EGFP were evaluated in the murine dendritic DC2.4 cell line. The mRNA release capacity and its postrelease translational efficiency were investigated for the most efficient LXs. Conformation of mRNA within 2/DOPE lipoplexes was determined. Lastly, mRNA transfection was compared with plasmid DNA transfection. Here, we evaluated mRNA transfection with cationic liposomes comprising either the lipid 2 or the α-amino-lipophosphonate featuring tertiary amine (3b) as cationic lipids associated with either DOPE or pH responsive α-amino-lipophosphonate lipids as colipids ( Figure 1 ) [33] . The membrane fusion activity of liposomes were measured at pH 7.4 and pH 5.5 and transfection efficiencies of lipoplexes with mRNA encoding EGFP were evaluated in the murine dendritic DC2.4 cell line. The mRNA release capacity and its postrelease translational efficiency were investigated for the most efficient LXs. Conformation of mRNA within 2/DOPE lipoplexes was determined. Lastly, mRNA transfection was compared with plasmid DNA transfection.

All the chemicals used in this study were purchased from Sigma (St. Quentin Fallavier, France) unless otherwise stated. Phosphate-buffered saline (PBS) was supplied by PAA Laboratories (Les Mureaux, France). O,O-dioleyl-N-histamine phosphoramidate (1) and (2) were Pharmaceutics 2022, 14, 581 3 of 16 synthesized as previously described [25, 26] . All α-amino-lipophosphonates were synthesized as previously described [33] .

Synthetic mRNA-EGFP and mRNA-Luc were produced as previously described [34] . pGEM4Z-EGFP-A64 and pGEM-4Z-Luc-A64 plasmids were linearized with SpeI. Linearized plasmids were used as a template for in vitro transcription using the mMessage mMachine T7 Transcription Kit (Ambion, Oxfordshire, UK) to produce anti-reverse cap analogue (ARCA)-A100 mRNAs. Synthetic mRNA-EGFP and mRNA-Luc had an ARCA modified cap and a 100-adenosine poly(A) tail. The RNA concentration was determined by measuring the absorbance at 260 nm; RNA had 260:280 ratios ≥2 and was stored at −80 • C in small aliquots. IVT mRNA-Luc was labelled with the fluorescein Label IT kit from MIRUS (Madison, WI, USA) and purified by ethanol precipitation.

pCMV-EGFP (5130 bp) was homemade plasmid DNA encoding the jellyfish Aequorea victoria enhanced green fluorescent protein (EGFP) under the control of the human cytomegalovirus (CMV) promoter. Supercoiled pDNA was isolated from Escherichia coli DH5 at supercompetent bacteria (Invitrogen, Cergy Pontoise, France) by alkaline lysis and purification was performed by using a QIAGEN EndoFree Plasmid Mega Kit (Qiagen, Courtaboeuf, France).

The murine dendritic cell line (DC2.4 cells) was kindly given by Dr P. Jeannin and Dr Yves Delneste (INSERM UMR 892 Nantes-Angers, France) [35] . They were grown at 37 • C in a humidified atmosphere that contains 5% CO 2 in Dulbecco's modified Eagle's medium (PAA Laboratories) supplemented with 10% fetal calf serum (PAA Laboratories) with 100 units per mL of antibiotics penicillin and 100 mg per mL of streptomycin (PAA Laboratories). The mycoplasma-free condition of the cells was evidenced using the MycoAlerts Mycoplasma Detection Kit (Lonza, Levallois Perret, France).

Liposomes were prepared by the film hydration method followed by sonication. For this, we used the required volume of a 3.5 mM or 1.75 mM co-lipid to 3.5 mM cationic lipid in either equimolar or 2/1 cationic/co-lipid ratio with absolute ethanol to the total volume of 500 mL in a round bottle flask. The solution was evaporated under vacuum in the rotary evaporator until a dry film remained at the base of the flask. The film was further hummed with nitrogen followed by the hydration with 10 mM HEPES buffer (pH 7.4) overnight at 4 • C. Finally, the dispersion was sonicated for 10 min at the room temperature at 37 kHz using a bath sonicator (Fischer Bioblock Scientific, Illkirch, France).

LX were prepared by upside down pipetting of mRNA or pDNA to liposomes at mRNA or DNA/liposomes charge ratio of 1/0.5, 1/1 and 1/2. For this, 2.5 µg mRNA or pDNA at 1 mg/mL were added to 0.75 µL, 1.5 µL and 3 µL of 5 mM liposomes in 10 mM HEPES buffer, pH 7.4, and the solution was kept stationary for 30 min at room temperature before being diluted in 0.5 mL of serum-free culture medium for transfection. Agarose gel (0.6% w/v) electrophoresis was performed to affirm the complex formation of mRNA or pDNA with liposomes at these ratios.

Liposomes and LX size distribution and ζ potential were measured at 25 • C on an SZ-100 Analyser (Horiba Scientific, les Ulis, France). For the analysis, the samples were prepared in cuvettes by adding 50 µL of the liposomes or LX solution to 1.45 mL of 10 mM HEPES buffer, pH 7.4. Size distribution by the hydrodynamic diameter was determined by dynamic light scattering using the photon correlation spectroscopy technique and calculated from the size distribution by volume (generated by the Stokes-Einstein equation for polydisperse samples), provided by the inbuilt software, and are reported as the average of three independent measurements ± the deviation from the mean. The uniformity of size distribution was recorded as the polydispersity index (PDI) obtained with the particle size. The ζ potential, the electrophoretic mobility of liposome dispersion, was recorded on the same instrument by using the zeta mode to monitor the global surface charge.

The membrane fusion potential of liposomes was measured using the fluorescence resonance energy transfer (FRET) assay as described [36] . N-(7-nitro-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (NBD-PE;) (Thermo Fisher Scientific, Les Ulis, France) and Rhodamine linked to 1,2-dihexadecanoyl-sn-glycero-3phosphoethanolamine (Rho-PE; Thermo Fisher Scientific) were used as donor and acceptor fluorescent lipids, respectively. 

Transfection was evaluated on the murine DC line (DC2.4 cells), one established clone from bone marrow of C57BW6 mice cultured in GM-CSF [37] . The cells were grown at 37 • C in a humidified atmosphere containing 95% air and 5% CO 2 in DMEM supplemented with 10% fetal calf serum (PAA Laboratories, Les Mureaux, France), 100 Units/mL of penicillin and 100 mg/mL of streptomycin (Fischer Bioblock, Illkirch, France). Two days before transfection, cells were seeded in 24 well culture plates at a density of 1 × 10 5 cells per cm 2 . At the time of transfection, cells were 80% confluent. The culture medium was discarded; cells were washed with serum-free medium and then incubated for 4 h at 37 • C with LX (2.5 µg of mRNA or pDNA diluted in 500 mL serum-free culture medium). Afterwards, the medium was removed and replaced with fresh medium containing 10% serum and cultured at 37 • C without any LX for 24 h and 48 h in the case of mRNA and DNA transfection, respectively. Finally, the cells were washed twice with PBS, harvested with trypsin, centrifuged (800× g for 5 min at 4 • C) and suspended in PBS. EGFP expression was quantified by flow cytometry (LSR, Becton Dickinson, Grenoble, France) by measuring the cell-associated fluorescence intensity at ň ex = 488 nm and ň em = 520 ± 24 nm.

48 h after transfection, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 100 mL of 5 mg per mL solution in PBS) was added to each well followed by incuba-Pharmaceutics 2022, 14, 581 5 of 16 tion at 37 • C for 4 h. MTT converted in Formazan was solubilized with acidic isopropanol and quantified by measuring the absorbance at 570 nm with a spectrophotometer. Cell viability was calculated for each treatment as "absorbance of LX treated cells/absorbance of control cells" × 100. Absorbance measured for the non-transfected cells cultured under the same conditions as those of transfected cells was used for the control cells. The results were presented as the mean of three experiments with each measurement in triplicate.

Fluorescein labelled mRNA-LXs were observed by using a Zeiss Axiovert 200 M microscope coupled with a Zeiss LSM 510 Meta scanning device (Carl Zeiss Co., Ltd., Iena, Germany). The inverted microscope was equipped with a Plan-Apochromat 63× objective (NA 1 /4 1.4) and with a temperature-controlled stage. Cells were seeded at 1 × 10 4 cells per wells in 0.5 mL of culture medium in a 4-well Lab-Tek chambered coverglass (Nunc, Dutsher S.A., Brumath, France). The day of transfection, cells were washed three times in a serum-free medium. Then, 0.5 mL of LX with 2.5 µg fluorescein labelled mRNA in serum-free medium was added and cells were incubated for 2 h at 37 • C. Then, cells were fixed in 3% p-formaldehyde in cold PBS.

In vitro (cell-free) luciferase activity was tested using a Rabbit Reticulocyte Lysate System, Nuclease treated (Promega, Madison, WI, USA) following the manufacturer's instruction. In 1.5 mL tube, 2.6 µL HEPES buffer (10 mM, pH 7.4) containing 0.5 µg of synthetic mRNA-Luc free, complexed with LX or upon DS treatment of LX were adjusted to the total volume of 7µL, and then mixed with 18µL of reticulocyte mixture and incubated for 1 h 30 min at 30 • C. Finally, 2.5 µL of this mixture was added to 50 µL of LAR (Luciferase Assay Reagent) and the luciferase activity was measured two times for 10 s with a 2 s interval using a luminometer (LUMAT LB 9507, Berthold, Wildbach, Germany).

CD spectra were recorded on a Jasco J-715 spectropolarimeter equipped with a Peltier device for the temperature control operating at 1.0 nm resolution. Quartz cells of suitable path length were used. Spectra are the average of at least four runs, performed in the range of 220-330 nm. All the spectra were normalized by the nucleotide concentration previously determined by absorption spectroscopy. CD spectra were normalized and reported as molar circular dichroism, ∆ε (M −1 cm −1 ).

The physicochemical characteristics of lipid 3b-based liposomes are presented in Table 1 . The average size was 147 nm (0.1< PDI < 0.5), the formulation 3b/3e featured the smallest size (85 nm) while the 3b/3a liposomes had the biggest size (235 nm). Their ζ potential averaged at 69 mV; 3b/3a (49 mV) and 3b/3c (56 mV) being less positively charged. For lipid 2-based liposomes, they had a size distribution of 104 nm in average (0.1< PDI < 0.5) with the biggest one at 207 nm (2/DOPE) and two intermediates at 148 nm (2/3d) and 163 nm (2/4b) ( Table 2 ). Their ζ potential averaged at 64 mV, the more positively charged being 2/1 at 81 mV. Table 1 . Physicochemical characterization of 3b-based liposomes.

Mol. Ratio £ Size (nm) PDI * ζ Potential (mV)

£ Molecular ratio 3b/co-lipid; * PDI stands for the polydispersity index. Values are means ± SD of three different measurements. Table 2 . Physicochemical characterization of 2-based liposomes.

£ Molecular ratio 2/co-lipid; * PDI stands for the polydispersity index. Values are means ± SD of three different measurements.

For the series 3b, the most fusogenic liposomes at acidic pH were those including co-lipids 3a or 1 ( Table 3) . The presence of an imidazole group close to the phosphonate of the co-lipid is not a determining factor when associated with lipid 3b. Other 3b-liposomes with co-lipids 3c or 4a featured an intermediate fusogenic behaviour. Finally, 3b-liposomes associated with co-lipids 3d, 3e, 4b or DOPE were not or only weakly fusogenic. Of note, the presence of a terminal pyridine as polar head group (3d and 4b) did not promote membrane fusion both at neutral and acidic pH. Overall, the presence of an imidazole in the head group of co-lipids of α-amino-lipophosphonates featuring two ionizable azaheterocycles (imidazole, 3a or pyridine, 4a) induced membrane fusion at pH 5.5. Liposomes of the series 2 were not or weakly fusogenic at neutral pH. Only liposomes with co-lipids 1 or DOPE were highly fusogenic at pH 5.5 which is the pH of the lumen of late endosomes. To sum-up, the presence of an imidazole group close to the phosphonate did not promote membrane fusion when associated with lipid 2. Moreover, the association of DOPE with lipid 2 and 3b at a lipid molar ratio of 1/1 and 2/1 increased the fusogenicity of liposomes at pH 5.5. Table 3 . Membrane fusion potential of liposomes (%).

Co-Lipids pH 7.4 pH 5.5 pH 7.4 pH 5.5 3a

Membrane fusion potential was calculated as percent of lipid inter-mixing extent (% fusion) after 15 min. DOTAP/DOPE (1/1) used as a control had fusion of 0 and~34% at pH 7.4 and pH 5.5, respectively. Values are means ± SD of three different measurements.

It is well known that the charge ratio of mRNA to liposomes (mRNA/L) directly influences the transfection efficiency of LX. Therefore, optimization of this ratio is crucial to find out the right balance between the efficacy and the cytotoxicity. Thus, in vitro transcribed (IVT) mRNA encoding EGFP was complexed with liposomes at three different mRNA/L ratios and used to transfect murine dendritic DC2.4 cells. Figure 2 shows an agarose gel electrophoresis of mRNA complexed with 2-based liposomes at different ratios. Membrane fusion potential was calculated as percent of lipid inter-mixing extent (% fusion) after 15 min. DOTAP/DOPE (1/1) used as a control had fusion of 0 and ~34% at pH 7.4 and pH 5.5, respectively. Values are means ± SD of three different measurements.

It is well known that the charge ratio of mRNA to liposomes (mRNA/L) directly influences the transfection efficiency of LX. Therefore, optimization of this ratio is crucial to find out the right balance between the efficacy and the cytotoxicity. Thus, in vitro transcribed (IVT) mRNA encoding EGFP was complexed with liposomes at three different mRNA/L ratios and used to transfect murine dendritic DC2.4 cells. Figure 2 shows an agarose gel electrophoresis of mRNA complexed with 2-based liposomes at different ratios.

For all tested mRNA/L ratios, the mRNA was quantitatively complexed with liposomes and no migration of free mRNA was observed ( Figure 2 ). The brightness of mRNA in the well decreased when the strength of the mRNA condensation in LX increased in line with liposomes quantity. Similar observations were found with mRNA complexed with 3b-based liposomes (data not shown). The transfection outcomes with the 3b-LXs indicated that the percentage of transfected cells with 3b/3e, 3b/4a, 3b/4b, 3b/DOPE LXs averaged around 30%, and the high of 40% was obtained with the two 3b/DOPE LXs, while it reached only 22% for 3b/3a, 3b/3c, 3b/3d and 3b/1 LXs ( Figure 3C ). The mRNA translation was quite low (MFI < 200) except for 3b/1 LX, which led to a high MFI value with the level being inversely proportional to the mRNA/L ratio (1000, 800 and 200 at 1/0.5, 1/1 and 1/2 mRNA/L ratio, respectively). Overall, formulations made with 3b and co-lipids 3d (one imidazole close to the phosphonate and a polar head with a terminal pyridine), 4b (one pyridine close to the phosphonate and a polar head with a terminal pyridine) and DOPE were not very efficient in terms of translation when compared to the 3b-LXs prepared with the co-lipid 1 (polar head with a terminal imidazole). The transfection outcome with 2-based LXs indicated that the highest transfection efficiencies were obtained at mRNA/L ratio of 1/0.5. Only a few formulations had a positive impact on the number of transfected cells (average around 40%; the highest with 2/1 LX being at 86%) and on the EGFP mRNA translation (MFI) level ( Figure 3D,E) . Overall, 2-based LXs allowed a higher number of transfected cells than the 3b-based LXs. For 2based LX, high transfections were obtained with co-lipids 3a (an imidazole close to the phosphonate and a polar head with a terminal imidazole), 3d (one imidazole close to the phosphonate and a polar head with a terminal pyridine), 1 (a polar head with a terminal imidazole) and DOPE.

The cationic lipid 3b having one imidazole close to the phosphonate and one terminal quaternary ammonium seems to accept only co-lipids that do not have a heterocycle (imidazole, pyridine) close to the phosphonate moiety. Conversely, the cationic lipid 2 accommodates co-lipids with imidazole close to the phosphonate and imidazole (3a) or pyridine (3d) at the terminal group.

The percentages of cell viability upon transfection with 3b-and 2-based LXs are shown in Table 4 . Transfection with 3b-based LXs was weakly cytotoxic as indicated by the high percentage of viable cells (>80%) except for 3b/3e LX that resulted in~30% of dead cells at an mRNA/L ratio of 1/1. It is worth noting that transfection with 3b/1 LX, the most efficient LX, resulted in 83 ± 6 and 100 ± 8% of viable cells at mRNA/L ratio of 1/0.5 and 1/1, respectively. For the 2-based LXs, cell viabilities depended on the nature of the co-lipid and on mRNA/L ratios. At mRNA/L ratio of 1/0.5, most of 2-based LX were not cytotoxic too (>80% viable cells), except for 2/4b and 2/1 LXs. 2/1 and 2/DOPE LXs showed high transfection efficacies. At an mRNA/L ratio of 1/1, 2/1 LX was more cytotoxic-~30% of viable cells-than at a ratio 1/0.5. In contrast, cytotoxicity remained low with 2/DOPE LX at ratio 1/1. The cytotoxicity of 2/1 LX could come from LX per se or free 2/1 liposomes. For 3b-based liposomes, the most efficient in terms of transfection were obtained with liposomes 3b/1 and the two 3b/DOPE. 3b/1 and 3b/DOPE (2/1) have a high membrane fusion potential at pH 5.5 of 71% and 58%, respectively. Despite the high membrane fusion potential of 3b/3a liposomes (79%, at pH 5.5), the corresponding LX did not promote high transfection. Among 2-based liposomes, the most efficient in terms of transfection were liposomes 2/3a, 2/3d, 2/1 and 2/DOPE, while the most fusogenic were 2/1 and 2/DOPE liposomes (Table 3 ). These results suggest that liposomes inducing more than 60% of membrane fusion at pH 5.5 lead to an interesting global transfection efficiency as observed for the formulations 3b/1, 2/1, and 2/DOPE. LXs made with 2/3a and 2/3d liposomes that are not or weakly fusogenic at pH 5.5 promote high transfection when used at mRNA/L ratio of 1/0.5. Altogether, these data indicate the absence of direct correlation between the transfection efficiencies and the liposome fusion properties. This is in line with the fact that the fusogenic potential of liposomes is likely impacted by its interaction with the mRNA. Fluorescence microscopy experiments were performed to observe the intracellular fate of mRNA upon delivery. Figure 4 shows confocal microscopy images recorded 2 h after incubation of the cells with LXs made with fluorescein-labelled IVT mRNA at mRNA/L 1/0.5 (the ratio giving the highest transfection efficiency as shown in Figure 3 ). Representative images of LXs made with fusogenic and non-fusogenic 3b liposomes were presented. For 2-series, only LXs with the most acid-fusogenic 2/1 and 2/DOPE liposomes are shown in this figure. Efficient mRNA delivery in the cytosol was evidenced by the presence of high diffuse fluorescence in the cytosol. This was observed for 3b LXs containing 3a, 3c, 3e, 4a or DOPE as co-lipids as well as for 2/1 LX. By contrast, the fluorescence staining with 3b/d was more compacted (vesicular) and less diffuse in the cytosol. The high acid-mediated membrane fusion of 3b-liposomes with co-lipids 3a (79%), 3c (41%), 4a (37%) and DOPE (58%) as well as liposomes 2/1 (97%) suggested that it contributed to endosome escape after endocytosis.

with the fact that the fusogenic potential of liposomes is likely impacted by its interaction with the mRNA. Fluorescence microscopy experiments were performed to observe the intracellular fate of mRNA upon delivery. Figure 4 shows confocal microscopy images recorded 2 h after incubation of the cells with LXs made with fluorescein-labelled IVT mRNA at mRNA/L 1/0.5 (the ratio giving the highest transfection efficiency as shown in Figure 3 ). Representative images of LXs made with fusogenic and non-fusogenic 3b liposomes were presented. For 2-series, only LXs with the most acid-fusogenic 2/1 and 2/DOPE liposomes are shown in this figure. Efficient mRNA delivery in the cytosol was evidenced by the presence of high diffuse fluorescence in the cytosol. This was observed for 3b LXs containing 3a, 3c, 3e, 4a or DOPE as co-lipids as well as for 2/1 LX. By contrast, the fluorescence staining with 3b/d was more compacted (vesicular) and less diffuse in the cytosol. The high acid-mediated membrane fusion of 3b-liposomes with co-lipids 3a (79%), 3c (41%), 4a (37%) and DOPE (58%) as well as liposomes 2/1 (97%) suggested that it contributed to endosome escape after endocytosis. In addition to endosomal escape, the mRNA must be released in an intact structure after the compaction by liposomes to be efficiently translated by ribosomes. Therefore, we investigated the mRNA release, translation capacity of post-release mRNA. Moreover, the mRNA conformation in the presence of liposomes was assessed. These investigations were conducted with 2/1 and 3b/1 liposomes (lipid molar ratio of 1/1) and 2/DOPE and 3b/DOPE liposomes (lipid molar ratio 2/1) which showed good transfection. 3b/DOPE and 2/DOPE LXs exhibited a similar size of 220 ± 10 nm and 200 ± 10 nm, respectively. 3b/DOPE LX was less positive than 2/DOPE with a ζ potential of 17 ± 1 mV and 33 ± 2 mV, respectively. 3b/1 LX were larger and less positive (260 ± 15 nm; 20 ± 1 mV) than 2/1 LX (160 ± 9 nm; 27 ± 2 mV). In addition to endosomal escape, the mRNA must be released in an intact structure after the compaction by liposomes to be efficiently translated by ribosomes. Therefore, we investigated the mRNA release, translation capacity of post-release mRNA. Moreover, the mRNA conformation in the presence of liposomes was assessed. These investigations were conducted with 2/1 and 3b/1 liposomes (lipid molar ratio of 1/1) and 2/DOPE and 3b/DOPE liposomes (lipid molar ratio 2/1) which showed good transfection. 3b/DOPE and 2/DOPE LXs exhibited a similar size of 220 ± 10 nm and 200 ± 10 nm, respectively. 3b/DOPE LX was less positive than 2/DOPE with a ζ potential of 17 ± 1 mV and 33 ± 2 mV, respectively. 3b/1 LX were larger and less positive (260 ± 15 nm; 20 ± 1 mV) than 2/1 LX (160 ± 9 nm; 27 ± 2 mV).

First, the release of mRNA from LXs incubated in the presence of dextran sulphate (DS) as polyanions was assessed by agarose gel shift assay. For 2/DOPE and 3b/DOPE LXs, there was a high release of mRNA (80-90% of mRNA) from LX upon incubation in the presence of 90 µM DS whatever the mRNA/L ratio used ( Figure 5 ).

First, the release of mRNA from LXs incubated in the presence of dextran sulphate (DS) as polyanions was assessed by agarose gel shift assay. For 2/DOPE and 3b/DOPE LXs, there was a high release of mRNA (80-90% of mRNA) from LX upon incubation in the presence of 90 µM DS whatever the mRNA/L ratio used ( Figure 5 ). Figure 5 . mRNA release from LX. mRNA was first complexed with (black bar) 2/DOPE (lipid molar ratio 2/1) or (white bar) 3b/DOPE (lipid molar ratio 2/1) liposomes at various mRNA/L ratio. Then, LX were incubated in the absence or the presence of 90 µM dextran sulphate of 500 kDa (DS) for 1 hr before electrophoresis on a 0.6% agarose gel. Gel running buffer was TAE (40 mM Tris-acetate, 1 mM EDTA) and mRNA gel was stained with ethidium bromide (0.5 µg/mL). mRNA fluorescence was revealed under UV lamp and quantified by image J. The percentage of mRNA release was determined from the intensity of mRNA that migrated after DS treatment of LX and the intensity of the same amount of free mRNA.

Next, in vitro transcription of mRNA was performed with mRNA coding luciferase complexed with 2/1, 2/DOPE, 3b/1, 3b/DOPE and also DOTAP/DOPE liposomes as reference. The experiments were conducted as a function of mRNA/L ratio before and after LX destabilization (i.e., mRNA release) in the presence of DS, as done above. Compared to free mRNA, the luciferase activity was impaired with the increased liposomes quantity (Figure 6 ). The decrease was 60% and 57% for 2/1 and 2/DOPE, respectively; it was 53% and 42% for 3b/1 and 3b/DOPE LXs at mRNA/L ratio 1/1. It looked less drastic than with DOTAP/DOPE LX (76%) at the same ratio. When LXs were treated with DS before in vitro transcription, the luciferase activity was partially recovered. The recovery was quasi complete at ratio 1/0.5 with 2/1, 2/DOPE, 3b/1 and 3b/DOPE LXs. Despite the efficient release of mRNA from both LXs, their transfection efficiency was different. 2/DOPE LXs were more efficient than 2/1, 3b/1 and 3b/DOPE ones; 2/1 and 3b/1 were close (Figure 3) . This difference could be due to the impact on the mRNA structure after interaction with liposomes. Indeed, the condensation of the mRNA could induce an irreversible denaturation of a part of mRNA, thus affecting its translation efficiency. We hypothesize that such a denaturation would increase when the amount of liposomes used in the formulation increased. Figure 5 . mRNA release from LX. mRNA was first complexed with (black bar) 2/DOPE (lipid molar ratio 2/1) or (white bar) 3b/DOPE (lipid molar ratio 2/1) liposomes at various mRNA/L ratio. Then, LX were incubated in the absence or the presence of 90 µM dextran sulphate of 500 kDa (DS) for 1 h before electrophoresis on a 0.6% agarose gel. Gel running buffer was TAE (40 mM Tris-acetate, 1 mM EDTA) and mRNA gel was stained with ethidium bromide (0.5 µg/mL). mRNA fluorescence was revealed under UV lamp and quantified by image J. The percentage of mRNA release was determined from the intensity of mRNA that migrated after DS treatment of LX and the intensity of the same amount of free mRNA.

Next, in vitro transcription of mRNA was performed with mRNA coding luciferase complexed with 2/1, 2/DOPE, 3b/1, 3b/DOPE and also DOTAP/DOPE liposomes as reference. The experiments were conducted as a function of mRNA/L ratio before and after LX destabilization (i.e., mRNA release) in the presence of DS, as done above. Compared to free mRNA, the luciferase activity was impaired with the increased liposomes quantity (Figure 6 ). The decrease was 60% and 57% for 2/1 and 2/DOPE, respectively; it was 53% and 42% for 3b/1 and 3b/DOPE LXs at mRNA/L ratio 1/1. It looked less drastic than with DOTAP/DOPE LX (76%) at the same ratio. When LXs were treated with DS before in vitro transcription, the luciferase activity was partially recovered. The recovery was quasi complete at ratio 1/0.5 with 2/1, 2/DOPE, 3b/1 and 3b/DOPE LXs. Despite the efficient release of mRNA from both LXs, their transfection efficiency was different. 2/DOPE LXs were more efficient than 2/1, 3b/1 and 3b/DOPE ones; 2/1 and 3b/1 were close ( Figure 3) . This difference could be due to the impact on the mRNA structure after interaction with liposomes. Indeed, the condensation of the mRNA could induce an irreversible denaturation of a part of mRNA, thus affecting its translation efficiency. We hypothesize that such a denaturation would increase when the amount of liposomes used in the formulation increased. 

To assess the occurrence of mRNA denaturation, we performed CD measurem of mRNA in the absence and the presence of increasing quantity of 2/DOPE liposo ( Figure 7A ). The CD spectrum of free mRNA showed a positive band at 222 nm wi shoulder at 235 nm, a negative minimum near 248 nm and positive peak at 267 nm. CD spectrum was typical to mRNA in the A-form double helix conformation to si stranded polynucleotides containing base stacking regions forming a double helix 39]. After complexation with the cationic liposomes, there is a decrease of the molar e ticity of the negative minimum near 248 nm and the positive peak at 267 nm, concomi with a small red shift, which increased with the mRNA/liposome ratio indicativ mRNA condensation. 

To assess the occurrence of mRNA denaturation, we performed CD measurements of mRNA in the absence and the presence of increasing quantity of 2/DOPE liposomes ( Figure 7A ). The CD spectrum of free mRNA showed a positive band at 222 nm with a shoulder at 235 nm, a negative minimum near 248 nm and positive peak at 267 nm. This CD spectrum was typical to mRNA in the A-form double helix conformation to single stranded polynucleotides containing base stacking regions forming a double helix [37] [38] [39] . After complexation with the cationic liposomes, there is a decrease of the molar ellipticity of the negative minimum near 248 nm and the positive peak at 267 nm, concomitant with a small red shift, which increased with the mRNA/liposome ratio indicative of mRNA condensation. 

To assess the occurrence of mRNA denaturation, we performed CD measurements of mRNA in the absence and the presence of increasing quantity of 2/DOPE liposomes ( Figure 7A ). The CD spectrum of free mRNA showed a positive band at 222 nm with a shoulder at 235 nm, a negative minimum near 248 nm and positive peak at 267 nm. This CD spectrum was typical to mRNA in the A-form double helix conformation to single stranded polynucleotides containing base stacking regions forming a double helix [37] [38] [39] . After complexation with the cationic liposomes, there is a decrease of the molar ellipticity of the negative minimum near 248 nm and the positive peak at 267 nm, concomitant with a small red shift, which increased with the mRNA/liposome ratio indicative of mRNA condensation. This conformational change in the presence of cationic liposomes was similar to that reported for mRNA with POPC/DOTAP liposomes showing a drastic denaturation of mRNA and a loss of translational activity [38] . Thus, we observed a nice correlation between CD spectra and protein expression in reticulocyte and transfection, which indicates that the compaction of mRNA during LX formation induced in turn an irreversible conformational change in mRNA, the so-called A-form [38, 40] , leading to decreased translation efficiency after intracellular release. To confirm this effect, a study should be carried out for other types of liposomes.

Last, we investigated the conformation of pDNA by CD spectra measurement in the absence and in the presence of increased quantity of 2/DOPE liposomes ( Figure 7B ). The CD spectrum of pDNA was characteristic to CD of B-type DNA with a negative band at 242 nm and a positive peak around 273 nm. Following the addition of cationic liposomes, the positive peak showed a marked intensity decrease accompanied by a strong red shift toward 283 nm while disappearing at a high DNA/liposomes ratio. The negative band at 242 nm progressively decreased and completely disappeared at the high DNA/liposomes ratio. These changes resulted from DNA compaction without denaturation when the amount of liposomes increased and were comparable to CD of calf-thymus DNA in the presence of DOTAP/DOPE liposomes. These changes resulted from DNA compaction without denaturation when the amount of liposomes increased and were comparable to CD of calf-thymus DNA in the presence of DOTAP/DOPE liposomes [41] . This is an important point to consider for all LXs.

Next, we compared the transfection efficiency of mRNA vs. pDNA as a function of liposomes and mRNA/L or pDNA/L ratio (Figure 8 ). 3b/4b, 2/4b, 3b/4a, 2/4a, 3b/3e, 2/3d, 2/DOPE and 3b/1 LXs allowed higher mRNA transfection at ratio 1/0.5. 3b/3d LX allowed better mRNA transfection at ratio 1/1. Overall, pDNA transfection was efficient at ratio 1/2 or 1/1, while that of mRNA transfection was at a ratio 1/0.5. In addition, the transfection level was often higher with mRNA than with DNA. For comparison, when lipofectamine (LFM) was used as the standard transfecting reagent, the global transfection (% * MFI) values were 28,138 and 8463 for mRNA and DNA transfection, respectively. This was probably related to their respective location of expression machinery. The pDNA must remain condensed until it reaches the nuclear envelope and released into the nucleus. Conversely, mRNA must be released into the cytosol and available to the ribosome machinery for translation. Thus, lipoplexes should be less stable with mRNA than with pDNA. mRNA and a loss of translational activity [38] . Thus, we observed a nice correlation between CD spectra and protein expression in reticulocyte and transfection, which indicates that the compaction of mRNA during LX formation induced in turn an irreversible conformational change in mRNA, the so-called A-form [38, 40] , leading to decreased translation efficiency after intracellular release. To confirm this effect, a study should be carried out for other types of liposomes. Last, we investigated the conformation of pDNA by CD spectra measurement in the absence and in the presence of increased quantity of 2/DOPE liposomes ( Figure 7B ). The CD spectrum of pDNA was characteristic to CD of B-type DNA with a negative band at 242 nm and a positive peak around 273 nm. Following the addition of cationic liposomes, the positive peak showed a marked intensity decrease accompanied by a strong red shift toward 283 nm while disappearing at a high DNA/liposomes ratio. The negative band at 242 nm progressively decreased and completely disappeared at the high DNA/liposomes ratio. These changes resulted from DNA compaction without denaturation when the amount of liposomes increased and were comparable to CD of calf-thymus DNA in the presence of DOTAP/DOPE liposomes. These changes resulted from DNA compaction without denaturation when the amount of liposomes increased and were comparable to CD of calf-thymus DNA in the presence of DOTAP/DOPE liposomes [41] . This is an important point to consider for all LXs.

Next, we compared the transfection efficiency of mRNA vs. pDNA as a function of liposomes and mRNA/L or pDNA/L ratio (Figure 8 ). 3b/4b, 2/4b, 3b/4a, 2/4a, 3b/3e, 2/3d, 2/DOPE and 3b/1 LXs allowed higher mRNA transfection at ratio 1/0.5. 3b/3d LX allowed better mRNA transfection at ratio 1/1. Overall, pDNA transfection was efficient at ratio 1/2 or 1/1, while that of mRNA transfection was at a ratio 1/0.5. In addition, the transfection level was often higher with mRNA than with DNA. For comparison, when lipofectamine (LFM) was used as the standard transfecting reagent, the global transfection (% * MFI) values were 28,138 and 8463 for mRNA and DNA transfection, respectively. This was probably related to their respective location of expression machinery. The pDNA must remain condensed until it reaches the nuclear envelope and released into the nucleus. Conversely, mRNA must be released into the cytosol and available to the ribosome machinery for translation. Thus, lipoplexes should be less stable with mRNA than with pDNA. 

This study reveals that the cationic α-aminolipophosphonate (3b) containing a tertiary aliphatic amine in the polar head group allows the efficient transfection of mRNA when formulated with either co-lipid 1 or DOPE. In addition, the imidazolium lipophosphoramidate, (2) when combined with co-lipid 3a, 3d, 1 or DOPE, also produces efficient formulations for mRNA delivery. Regarding the membrane fusion activity in acidic medium of the respective liposomes, it appears that the level of mRNA translation is not always linked only to an endosome leak occurring after the protonation of these co-lipids. When comparing mRNA transfection vs. pDNA transfection, mRNA transfection is higher, with a lower quantity of cationic liposomes. This is probably explained by the presence of denatured mRNA in LXs when the quantity of cationic liposomes increased, as shown by CD for 2/DOPE LX, in contrast to DNA. This study leads to the conclusion that the mRNA/liposomes ratio is a key parameter that must be considered with care. Indeed, the compaction is needed to protect mRNA in the extracellular medium and to favor cell internalization, but a too strong compaction produces conformation changes of mRNA that negatively affects the translation efficacies.

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The authors declare no conflict of interest.Pharmaceutics 2022, 14, 581 

Funding: This research was funded by Région Centre Val de Loire, France (ARD2020 Biomédicaments and APR of regional interest VACARME.

The authors declare no conflict of interest. 

This study reveals that the cationic α-aminolipophosphonate (3b) containing a tertiary aliphatic amine in the polar head group allows the efficient transfection of mRNA when formulated with either co-lipid 1 or DOPE. In addition, the imidazolium lipophosphoramidate, (2) when combined with co-lipid 3a, 3d, 1 or DOPE, also produces efficient formulations for mRNA delivery. Regarding the membrane fusion activity in acidic medium of the respective liposomes, it appears that the level of mRNA translation is not always linked only to an endosome leak occurring after the protonation of these co-lipids. When comparing mRNA transfection vs. pDNA transfection, mRNA transfection is higher, with a lower quantity of cationic liposomes. This is probably explained by the presence of denatured mRNA in LXs when the quantity of cationic liposomes increased, as shown by CD for 2/DOPE LX, in contrast to DNA. This study leads to the conclusion that the mRNA/liposomes ratio is a key parameter that must be considered with care. Indeed, the compaction is needed to protect mRNA in the extracellular medium and to favor cell internalization, but a too strong compaction produces conformation changes of mRNA that negatively affects the translation efficacies. Funding: This research was funded by Région Centre Val de Loire, France (ARD2020 Biomédicaments and APR of regional interest VACARME.