key: cord-0840215-kch5yfui authors: Kadmon, Gili; Levy, Itzhak; Mandelboim, Michal; Nahum, Elhanan; Stein, Jerry; Dovrat, Sara; Schonfeld, Tommy title: Polymerase‐chain‐reaction‐based diagnosis of viral pulmonary infections in immunocompromised children date: 2013-03-28 journal: Acta Paediatr DOI: 10.1111/apa.12207 sha: 99eb0431d4cb92d25f599d2a4ab953a6d9aeff37 doc_id: 840215 cord_uid: kch5yfui AIM: Viral pneumonia is a serious complication in immunocompromised children. Its aetiology is difficult to identify owing to the limitations of conventional microbiological tests. The aim of this study was to determine whether polymerase chain reaction (PCR) assays for respiratory viruses increase the diagnostic yield of bronchoalveolar lavage (BAL) in immunocompromised children. METHODS: BAL samples obtained from immunocompromised children hospitalized with pneumonia were processed for respiratory viruses by viral culture, rapid antigen test and PCR (for CMV, adenovirus, influenza, parainfluenza, herpesvirus, RSV and hMPV). RESULTS: The study group included 42 patients (mean age 7.2 ± 5.1 years) with 50 episodes of clinical pneumonia (50 BAL samples). Forty viral pathogens were identified in 30 episodes (60%). PCR increased the diagnostic rate by fourfold (75% identified by PCR alone, p < 0.0001). When viral culture and rapid antigen test were used as the gold standard, PCR was found to have high sensitivity (86–100% when assessed) and specificity (80–96%). The PCR results prompted the initiation of specific antiviral therapy and the avoidance of unnecessary antibiotic treatment in 17 (34%) episodes. CONCLUSION: PCR‐based diagnosis from BAL may increase the rate of pathogen detection in immunocompromised children, decrease the time to diagnosis and spare patients unnecessary antimicrobial treatment. INTRODUCTION Viral pneumonia is a serious infectious complication in immunocompromised children and adults. It is associated with high rates of morbidity, including respiratory and multiorgan failure, increased rates of hospitalization and need for intensive care, and mortality rates of up to 50% (1) (2) (3) (4) . Diagnosis is often difficult because the clinical and radiological findings lack sensitivity and specificity (4, 5) ; viral cultures are time-consuming and have low sensitivity (1, 4, 5) ; and direct antigen assays lack sensitivity and, although they allow for rapid diagnosis, are unavailable for a wide range of viral pathogens (1) (2) (3) (4) (5) (6) (7) . In recent years, molecular techniques of gene amplification have been applied for the rapid and sensitive detection of infectious agents. Polymerase chain reaction (PCR) assays for viral respiratory pathogens were shown to have both high sensitivity and specificity (1) (2) (3) (4) (5) (6) (7) (8) (9) . Studies reported a two-to threefold increase in the diagnostic yield when PCR was added to the standard microbiological investigations (1) (2) (3) (4) 6, 7, 10) . In different studies, only 20-42% of cases that were virus-positive by PCR were also positive by viral culture (1, 4, 5) ; the positive rate for rapid antigen tests was about 40% of viral PCR-positive cases (3, 4) . In children, several studies have validated the use of PCR for the detection of viral pathogens, including influenza virus, parainfluenza virus, adenovirus, respiratory syncytial virus Abbreviations BAL, bronchoalveolar lavage; BMT, bone marrow transplantation; PCR, polymerase chain reaction; RSV, respiratory syncytial virus. In immunocompromised children with pneumonia, viral PCR assays, as compared with standard microbiological assays (viral cultures and rapid antigen tests) from bronchoalveolar lavage, increased the diagnosis yield by fourfold. When viral culture and rapid antigen test were used as the gold standard, PCR was found to have high sensitivity and specificity. PCR assays also supported the discontinuation of unnecessary antibiotic treatment in 34% of episodes. (RSV), human metapneumovirus (hMPV), rhinovirus, coronavirus and herpesvirus (4, 7, 9, 10) . However, data on immunocompromised children in particular are still sparse, especially for PCR tests of bronchoalveolar lavage (BAL) fluid. The aim of the present study was to determine whether PCR tests for respiratory viruses add to the diagnostic yield of BAL in immunocompromised children with pneumonia. This retrospective study was conducted in the paediatric intensive care unit of Schneider Children's Medical Center of Israel, a tertiary care, university-affiliated, 250-bed paediatric facility. All infants and children with primary or secondary immunodeficiency who were hospitalized with pneumonia between January 2007 and June 2009 needing BAL were included in the study. Only episodes in which BAL samples were analysed using both the standard microbiological tests (culture, rapid antigen tests) and PCR-based assays were included in the analysis. The study was approved by the Institutional Review Board of Rabin Medical Center which waived the need for informed consent. The patients' medical files were reviewed for the following data: age and sex, underlying disease and predisposing factors, duration of hospitalization in the intensive care unit, prior viral infections, presence of fever and respiratory distress, laboratory data (blood cell count, chemistry results), radiological findings, antimicrobial therapy before and after BAL fluid analysis, results of BAL fluid analysis, clinical decisions based on BAL test results and clinical outcome of each episode. The present study was conducted simultaneously with a study of fungal pathogens using PCR and standard tests (fungal culture, calcofluor staining and immunofluorescence staining for Pneumocystis jirovecii) in the same cohort (11) . BAL procedure BAL was performed in the bronchoscopy suite within the paediatric intensive care unit. Patients were prepared for the procedure with inhalation of adrenaline and lidocaine and sedated with midazolam, atropine and propofol. A fiberoptic bronchoscope was wedged into a subsegmental bronchus of the affected lobe/lobes, and 5-15 mL aliquots of sterile normal saline solution were aspirated. The BAL fluid was transferred for examination. Each sample of BAL fluid was tested for the presence of adenovirus, cytomegalovirus (CMV), parainfluenza virus and herpesvirus, RSV, adenovirus and influenza virus. Realtime PCRs were performed (using the TaqMan probe detection system) for adenovirus, CMV, herpesvirus, and real-time reverse transcriptase PCR assays were performed for influenza virus, RSV and hMPV. Reverse transcriptase PCRs were used for parainfluenza virus. Viral cultures and rapid antigen tests (for RSV) were performed in the Microbiology Department of Rabin Medical Center. The PCR assays were performed in the Central Virology Laboratory of Chaim Sheba Medical Center. Adenovirus was detected through the amplification of a conserved region in the hexon gene (12) . To detect HSV-1 infection, the junction between glycoprotein G/J was amplified. HSV-2 was detected by amplification of glycoprotein G (13) . For influenza A virus, specific primers were selected to amplify part of the influenza virus M-protein gene and for influenza B virus, and specific primers were designated to amplify part of the HA gene (14) . For the detection of RSV A and B, the N gene of RSV was chosen as the target (15), and for hMPV the L gene was selected (16) . Parainfluenza 1, 2 and 3 viruses were detected using multiplex RT-PCR assays that detect and differentiate (17, 18) . Viral genomic DNA was extracted from the BAL fluid with a QIAamp DNA Blood Mini kit (Qiagen GmbH, Hilden, Germany), and viral genomic RNA was extracted with a High Pure Viral RNA kit (Roche Diagnostics GmbH, Mannheim, Germany). To test for CMV, DNA was extracted from 200 lL BAL fluid using the MagNaPure LC Total Nucleic Acid Isolation kit (Roche Diagnostics), according to the manufacturer's instructions. The ABI 7500 Real-Time PCR system (Applied Biosystems, Foster City, CA, USA) was used. One primer set was designed from the highly conserved region of glycoprotein B, the major envelope glycoprotein of the CMV gene, and a second set was designed from the highly conserved immediate early region (19, 20) . Immunosuppression was defined as current treatment of chemotherapy or immunosuppressive therapy, bone marrow transplantation or known primary immune deficiency. Clinical pneumonia was diagnosed when at least two of the following four criteria were met: fever, a new pulmonary infiltrate, worsening of pulmonary infiltrates and dyspnoea. The indication for BAL was clinical pneumonia that did not respond to empirical therapy within 48-72 h. The decision to add PCR testing was based on patient history, findings on physical examination, history of viral infections, radiological findings and presence of specific risk factors. Clinical improvement was defined as resolution of at least two of three diagnostic criteria (fever, respiratory symptoms and pulmonary infiltrates on chest films) within 2 weeks of BAL. The diagnostic rates of the standard microbiological methods (viral culture, rapid antigen test) and PCR were calculated as the percentage of viral pathogens identified by each method. The differences in diagnostic rates between the standard methods and PCR were analysed by chi-square test. Culture and rapid antigen test served as the reference (gold standard) for calculating PCR sensitivity and specificity for each viral pathogen. Positive and negative predictive values were not calculated because of the low number of positive findings for each pathogen. Episodes of respiratory illness were divided into two groups: those diagnosed by standard methods (with positive or negative PCR results) and those diagnosed by PCR alone. The groups were compared for percentage of episodes in which clinical improvement occurred out of the total episodes in each group and the percentage of patients who died during the same hospitalization out of the total number of patients in each group. Differences in rates of clinical improvement and mortality between the two groups were analysed by chisquare test. A p value of 0.05 was considered significant. The study cohort included 42 patients with a total of 50 episodes of clinical pneumonia (50 BAL samples). The patient characteristics are presented in Table 1 . In 46 episodes (92%), antibiotic treatment was started prior to BAL because of a suspected bacterial pulmonary infection. In I5 episodes (30%), antiviral therapy (ribavirin, cidofovir or ganciclovir) was also started prior to BAL because of a suspected viral pulmonary infection, based on previous infection (weeks to months before the current pneumonia episode), with CMV and/or adenovirus in most cases. In additional four episodes, patients were receiving antiviral prophylaxis with acyclovir at the time of BAL. In 31 episodes, patients were treated with antifungal therapy (i.e. amphotericin, voriconazole or fluconazole) at the time of BAL examination. Samples from 20 episodes (40%) were negative for a viral antigen by both standard tests (culture and rapid antigen test) and PCR. In 30/50 episodes (60%), a viral pathogen was identified. In these 30 episodes, 40 viral pathogens were identified ( Table 2 ). Ten of the pathogens (25%) were isolated by standard tests (viral culture, nine pathogens; rapid antigen test, 1 pathogen -RSV), with either negative or positive results on PCR assays. The other 30 pathogens (75%, p < 0.0001) were detected by PCR alone. The addition of PCR to the BAL evaluation increased the diagnostic yield by fourfold. The pathogen most often detected was adenovirus, in 12 episodes (24%), followed by CMV in nine episodes (18%), RSV in eight episodes (16%), Influenza virus in four episodes (8%, Influenza B in three episodes and A in one episode), parainfluenza in four episodes (8%) and herpesvirus two in three episodes (6%). RSV and Influenza were positive in episodes occurring during the winter and early spring months. None of the episodes was positive for hMPV. When viral culture and rapid antigen test were used as the gold standard, PCR was found to have high sensitivity (86-100% when assessed) and specificity (80-96%). Of the 30 episodes positive for viral infection, coinfection with other pathogens was observed in 18 (Table 3) : bacterial pathogens in 13 episodes (43%, as compared with 28% in viral-negative episodes), other viral pathogens in eight episodes (27%) and fungal pathogens in six episodes (20%, as compared with 28% in viral-negative episodes). In 18 of the 20 episodes (90%) in which findings for all three diagnostic methods were negative, empiric antiviral therapy was discontinued or withheld (36% of all episodes included in the study). Among the 30 episodes in which a viral pathogen was identified, antiviral therapy was initiated or continued in 17 (57%). Treatment in these cases consisted of ganciclovir for CMV infection (four episodes), foscarnet for CMV infection (four episodes), adenovirus infection (one episode), cidofovir for adenovirus infection (six episodes), ribavirin for RSV infection (one episode) and valganciclovir for CMV infection (one episode). On the basis of the diagnosis of a viral infection, empiric antibiotic treatment was discontinued or withheld in 17 episodes (36%). Clinical improvement was noted in 28 of the 47 episodes (60%) for which follow-up data were available. The 47 episodes included 18 of the 20 episodes diagnosed by PCR alone. Within this subgroup, clinical improvement was observed in 10 episodes (56%, compared with 43% for episodes diagnosed by standard methods, p = 0.34). Nine patients died during the same hospitalization in which BAL was performed (18% in-hospital mortality). These included 4 of the 20 patients (and 20 episodes) diagnosed by PCR alone. The in-hospital mortality rate of PCR-based diagnosis group was lower than the mortality rate of the standard methods-diagnosed group (20% and 37%, respectively, p = 0.54). In the present study, BAL samples from immunocompromised children with clinical pneumonia were tested by PCR assay in addition to standard microbiological methods of viral culture and rapid antigen test. Compared with the standard tests, PCR had high rates of sensitivity (86-100%, when assessed) and specificity (78-96%). The addition of PCR increased the rate of diagnosis of each viral pathogen tested, especially adenovirus, RSV, influenza virus and parainfluenza virus and significantly increased (by fourfold) the diagnostic yield of BAL sampling (30 pathogens identified by PCR alone vs 10 pathogens identified by standard methods). PCR assays also promoted initiation or continuation of specific anti-viral therapy in 17 of the 30 positive episodes. To the best of our knowledge, this is the only published study to compare effectiveness of viral PCR to standard microbiological methods using BAL fluid from immunocompromised children. Our 60% rate of diagnosis of viral infections with the addition of PCR is consistent with previous findings in immunocompromised patients (38-80%) (1,3,4,6). Gerna and colleagues found that 50% of all respiratory infections in adult lung transplant recipients were positive for PCR sensitivity could not be assessed, as no episode was positive for the same virus by standard and PCR assays. **PCR sensitivity and specificity could not be assessed, as no conventional assay for parainfluenza was done. (4) . Accordingly, viral infections appear to play a very significant role in immunocompromised patients. The information provided by the PCR assays was also important in preventing unnecessary treatment. Antiviral treatment was discontinued or withheld in 18 of the 20 episodes (90%) in which both standard tests and PCR yielded negative findings. Antibiotic therapy was discontinued or withheld in 17 of 30 episodes (57%) in which a viral pathogen was identified. It is noteworthy that bacterial cultures from BAL may have been largely negative because in 46 of the 50 episodes, patients were already treated with antibiotics at the time of BAL. The detection of a viral pathogen by PCR further decreased the likelihood of bacterial infection and supported the avoidance of antibiotic therapy. Antibiotic therapy was discontinued after 7-10 days in most cases. This was the average time between the occurrence of fever and the receiving of PCR results of the BAL (which was done 2-3 days after antibiotic treatment was started in most cases). Co-infection of viral pathogen with bacterial, viral and fungal pathogens was common in our study (20-43%). The two most common viruses in co-infections were RSV and adenovirus. Co-infection has been reported in other studies of viral PCR assays, especially in children and immunocompromised patients, with rates of up to 50% (2,3,7). A possible pitfall in relying on PCR assays is the risk of false positives. As healthy subjects may carry viral particles in their respiratory secretions (mostly due to a previous infection), viral PCR assays may sometimes yield positive results even in the absence of respiratory illness. (4) Thavagnanam and colleagues tested non-bronchoscopic BAL samples from healthy and asthmatic children by PCR assays for 13 common viral pathogens. The rate of positive results in the children without a respiratory illness was 35% (21) . However, this problem may be less relevant in immunocompromised patients. In one study of adult lung transplant recipients, the false-positive rate of PCR assays for viral pathogens was only 4% in the patients with no respiratory symptoms compared with 55% in the patients with a respiratory tract infection. When BAL samples taken after the respiratory illness were evaluated for the same viral pathogens by PCR, all were negative. These findings suggest that respiratory viruses do not cause latent infections (1). In the present study, we could not accurately evaluate the false-positive rate because the PCR results were analysed against standard tests which are known to have low sensitivity. Nevertheless, although the findings were not statistically significant, patients in whom treatment was directed solely by the (positive) PCR results had higher rates of clinical improvement and lower mortality. We could not have expected this finding had some of the PCR results been falsely positive, supporting the assumption that the falsepositive rate of PCR is low. Another limitation is the potential risks of BAL. Bronchoscopy is an invasive procedure which carries several risks, especially in immunocompromised patients with respiratory disease, among them are bleeding and respiratory failure. In our study, bronchoscopy was done in the paediatric intensive care unit under sedation (not general anaesthesia) and no complications were observed. There were also no cases of mortality in the 10 days following the procedure. However, the benefit of BAL should be considered against the potential risk of the procedure in every patient. Other limitations of the study are the retrospective, single-centre design of the study, the relatively small number of episodes evaluated and the heterogeneity of the patients (e.g. primary immunodeficiency, bone marrow transplantation patients). In addition, some PCR assays were not available to us, including coronavirus and rhinovirus, which are increasingly acknowledged to be significant pathogens in patients with respiratory tract infections, especially immunocompromised patients. It is possible that the diagnostic yield would have been even higher with these additional examinations. In conclusion, viral PCR assays increased the diagnostic yield of BAL in immunocompromised children with pneumonia, prompted the initiation of specific antiviral treatment and spared patients unnecessary treatment. 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