key: cord-0841260-9zyt0xvx authors: Brumme, Zabrina L.; Mwimanzi, Francis; Lapointe, Hope R.; Cheung, Peter; Sang, Yurou; Duncan, Maggie C.; Yaseen, Fatima; Agafitei, Olga; Ennis, Siobhan; Ng, Kurtis; Basra, Simran; Lim, Li Yi; Kalikawe, Rebecca; Speckmaier, Sarah; Moran-Garcia, Nadia; Young, Landon; Ali, Hesham; Ganase, Bruce; Umviligihozo, Gisele; Omondi, F. Harrison; Atkinson, Kieran; Sudderuddin, Hanwei; Toy, Junine; Sereda, Paul; Burns, Laura; Costiniuk, Cecilia T.; Cooper, Curtis; Anis, Aslam H.; Leung, Victor; Holmes, Daniel; DeMarco, Mari L.; Simons, Janet; Hedgcock, Malcolm; Romney, Marc G.; Barrios, Rolando; Guillemi, Silvia; Brumme, Chanson J.; Pantophlet, Ralph; Montaner, Julio S.G.; Niikura, Masahiro; Harris, Marianne; Hull, Mark; Brockman, Mark A. title: Humoral immune responses to COVID-19 vaccination in people living with HIV receiving suppressive antiretroviral therapy date: 2021-10-15 journal: medRxiv DOI: 10.1101/2021.10.03.21264320 sha: 8d32047e697da816ddf13cd9aff518a9b499b02a doc_id: 841260 cord_uid: 9zyt0xvx Humoral responses to COVID-19 vaccines in people living with HIV (PLWH) remain incompletely understood. We measured circulating antibodies against the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein, ACE2 displacement and live viral neutralization activities one month following the first and second COVID-19 vaccine doses in 100 adult PLWH and 152 controls. All PLWH were receiving suppressive antiretroviral therapy, with median CD4+ T-cell counts of 710 (IQR 525–935) cells/mm(3). Nadir CD4+ T-cell counts ranged as low as <10 (median 280; IQR 120–490) cells/mm(3). After adjustment for sociodemographic, health and vaccine-related variables, HIV infection was significantly associated with 0.2 log(10) lower anti-RBD antibody concentrations (p=0.03) and ~11% lower ACE2 displacement activity (p=0.02), but not lower viral neutralization (p=0.1) after one vaccine dose. Following two doses however, HIV was no longer significantly associated with the magnitude of any response measured. Rather, older age, a higher burden of chronic health conditions, and having received two ChAdOx1 doses (versus a heterologous or dual mRNA vaccine regimen) were independently associated with lower responses. After two vaccine doses, no significant correlation was observed between the most recent or nadir CD4+ T-cell counts and vaccine responses in PLWH. These results suggest that PLWH with well-controlled viral loads on antiretroviral therapy and CD4+ T-cell counts in a healthy range will generally not require a third COVID-19 vaccine dose as part of their initial immunization series, though other factors such as older age, co-morbidities, vaccine regimen type, and durability of vaccine responses will influence when this group may benefit from additional doses. Further studies of PLWH who are not receiving antiretroviral treatment and/or who have low CD4+ T-cell counts are needed. As people living with HIV (PLWH) may be at increased risk for severe possibly as a result of immunosuppression, higher rates of multi-morbidity and social determinants of health [1] [2] [3] [4] , COVID-19 vaccination is expected to benefit this group 5 . Our understanding of immune responses to COVID-19 immunization in PLWH however remains limited, in part because relatively few PLWH were included in the clinical trials for the COVID-19 vaccines that have now been widely administered in Canada and Europe (~196 for the BNT162b2 mRNA vaccine 6, 7 , 176 for the mRNA-1273 mRNA vaccine 8 and 54 and 103 PLWH respectively in the UK and South Africa for the ChAdOx1 viral vectored vaccine 9 ). Furthermore, immune response data from PLWH in these trials are currently only available for ChAdOx1 10, 11 . "Real-world" COVID-19 vaccine immune response data from PLWH are also limited. While all three of these vaccines have shown effectiveness following their initial mass rollouts [12] [13] [14] , and while clinical trial and observational data have shown robust vaccine-induced humoral immune responses in the general population [15] [16] [17] , impaired responses have been reported in certain immunocompromised groups including solid organ transplant recipients 18, 19 , cancer patients [20] [21] [22] , and individuals on immunosuppressive or immune-depleting therapies [23] [24] [25] . While antiretroviral therapy durably suppresses HIV to undetectable levels in plasma, restores CD4+ T-cell numbers, and can reverse HIV-induced immune dysfunction to a substantial extent [26] [27] [28] [29] , persistent immunopathology can nevertheless lead to blunting of immune responses to vaccination in PLWH [30] [31] [32] . Though "real world" COVID-19 vaccine immunogenicity data in PLWH are emerging [33] [34] [35] [36] , these studies have featured limited numbers of PLWH and/or controls, and none have adjusted for chronic health conditions that may impair immune responses 37 . Here, we characterize SARS-CoV-2-specific humoral immune responses 4 after immunization with one and two doses of a COVID-19 vaccine in 100 PLWH and 152 control participants ranging from 22 to 88 years of age. Characteristics of the 100 PLWH and 152 controls are shown in Table 1 PLWH and controls were similar in terms of age, but were different in terms of sex and ethnicity, with the PLWH group including more males and white ethnicity ( Table 1 ). PLWH and controls had similar numbers of chronic health conditions (median 0; IQR 0-1; range 0-3 in both groups); the most common conditions were hypertension and asthma. At study entry, 8% of PLWH and 10% of controls were identified as COVID-19 convalescent based on the presence of anti-N antibodies. An additional one (1%) PLWH and two (1.5%) controls developed anti-N antibodies during follow-up consistent with SARS-CoV-2 infection after one vaccine dose. These participants were retained in the "COVID-19 naive at study entry" group, as excluding them did not affect results (not shown). 5 All participants received two COVID-19 vaccine doses between December 2020 and August 2021, with 97% of controls receiving an mRNA vaccine for their first dose compared to 83% of PLWH ( Table 1) . This is because health care workers, who represent 59% of controls, were eligible for vaccination before ChAdOx1 was approved in Canada, while members of the public, including PLWH, received the vaccine(s) recommended for their age group during the mass rollout. More PLWH received heterologous (ChAdOx1/mRNA) regimens compared to controls (8% and 2%, respectively). Heterologous regimens were administered in Canada after mRNA vaccines were universally recommended as second doses 38 , after reports of rare thrombotic events associated with the ChAdOx1 vaccine emerged 39 . The between-dose interval was also longer for the controls (median 89 days, versus 58 for PLWH). This is because the province of British Columbia (BC) extended the dose interval to 112 days beginning on March 1, 2021 due to limited vaccine supply 40 , which meant that health care workers who were vaccinated around that time waited the longest for their second doses, while those vaccinated later waited a shorter time, as supplies increased. Samples were collected prior to vaccination where possible (66% of PLWH and 97% of controls), one month after the first vaccine dose (98% of both PLWH and controls) and one month after the second dose (96% of PLWH and 99% of controls). Among participants naive to COVID-19 at study entry, all but three (one PLWH and two controls) developed anti-RBD antibodies after one vaccine dose, though overall concentrations in Figure 1A ). In contrast, and consistent with prior studies demonstrating robust immune responses after one vaccine dose in previously infected individuals 41, 42 , anti-RBD antibody concentrations in COVID-19 convalescent participants (median 3.91 log 10 U/mL) were >2 log 10 higher than in the COVID-19 naive PLWH or control participants (both p<0.0001) (convalescents were analyzed as a single group, since there was no statistically significant difference between PLWH and controls in this category; Mann-Whitney p=0.17). In multivariable analyses controlling for sociodemographic, health and vaccine-related variables, the strongest independent predictors of lower antibody responses after one dose were older age (every decade of age associated with an adjusted ~0.1 log 10 lower response; p=0.0002), and a higher number of chronic health conditions (every additional condition associated with an adjusted 0.14 log 10 lower response; p=0.0058) ( Table 2) . HIV infection was also associated with an adjusted 0.2 log 10 lower antibody response after one vaccine dose (p=0.031). Prior COVID-19 was associated with an adjusted 1.88 log 10 higher response after one dose (p<0.0001). The second vaccine dose substantially boosted anti-RBD binding antibody concentrations in all but two participants: one PLWH with immunodeficiency due to a chronic blood disorder, and one >80 year old control participant with three chronic health conditions ( Figure 1B) . Overall, the second dose boosted anti-RBD levels in COVID-19 naïve individuals, both PLWH and controls, by a median of >2 log 10 , but in COVID-19 convalescent participants only marginally (median 0.14 log 10 ), compared to that measured one month after the first dose (Supplemental Figure 1B) CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not certified by peer review) The copyright holder for this preprint this version posted October 15, 2021. ; 7 log 10 lower than in convalescent participants (median 4.13 [IQR 3.87-4.29] log 10 U/mL; Mann-Whitney p=0.02) ( Figure 1B) . In multivariable analyses, HIV infection was no longer associated with antibody concentrations after two vaccine doses (p=0.91, Table 2 ). Rather, older age, a greater number of chronic conditions and having received two ChAdOx1 doses were independently predictive of weaker responses, with every 10 years of older age, each additional chronic condition and having received dual ChAdOx1 doses associated with 0.052 log 10, 0.11 log 10 and 0.64 log 10 lower antibody concentrations, respectively (all p<0.02). A longer dose interval was also associated with marginally higher antibody concentrations (0.023 log 10 per additional week, p=0.049). After two doses, there was no longer a significant association between prior COVID-19 infection and antibody response (p=0.50). Among PLWH who were naive to COVID-19 at study entry, we observed a weak positive relationship between the most recent CD4+ T-cell count and antibody concentration after one dose that was not statistically significant (Spearman's correlation ρ=0.18, p=0.09), but no significant relationship after the second dose (Spearman's ρ=0.11, p=0.3; Figure 1C ). Similarly we observed a weak positive relationship between nadir CD4+ T-cell count and antibody concentration after one dose that was not statistically significant (Spearman's ρ=0.19, p=0.07), but no significant relationship after the second dose (Spearman's ρ=0.05, p=0.6) (Supplemental Figure 2A) . We next assessed the ability of plasma to block the RBD-ACE2 interaction, which represents a higher throughput approach to estimate potential viral neutralization activity (also . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not certified by peer review) The copyright holder for this preprint this version posted October 15, 2021. ; https://doi.org/10.1101/2021.10.03.21264320 doi: medRxiv preprint 8 referred to as a surrogate viral neutralization test, sVNT 43 ). After one vaccine dose, PLWH and controls who were COVID-19 naive at study entry exhibited median 44% (IQR 27-64%) and 58% (IQR 47-71%) ACE2 displacement activities, respectively, indicating lower function among PLWH (Mann-Whitney p<0.0001) (Figure 2A) . In contrast, convalescent participants exhibited a median 99.7% (IQR 97.8-99.9%) ACE2 displacement activity after one vaccine dose (Mann-Whitney p<0.0001 compared to both naive groups). In multivariable analyses, HIV infection remained significantly associated with an adjusted 11% lower ACE2 displacement activity after one vaccine dose (p=0.023), with male sex (adjusted ~7% lower activity compared to female sex, p=0.031) and having received ChAdOx1 as the first dose (adjusted 18.8% lower activity compared to an mRNA vaccine as first dose, p=0.0001) remaining additional independent predictors of lower ACE2 displacement activity. Prior COVID-19 remained associated with an adjusted 36% higher ACE2 displacement activity following one vaccine dose (p<0.0001). Following two vaccine doses, the median ACE2 displacement activity in COVID-19 naive PLWH and controls rose to >95% in both groups and there was no longer a statistically remained statistically significantly higher than both naïve groups (both p<0.02), the magnitude of this difference was marginal. In fact, the second dose boosted ACE2 displacement activities in PLWH to an overall greater extent than in controls (Supplemental Figure 1C, D) . Consistent with this, multivariable analyses identified older age, a larger number of chronic conditions, and dual ChAdOx1 vaccination − but not HIV − as being independently associated with lower ACE2 displacement function after two vaccine doses (adjusted 1.6% lower ACE2 displacement . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 15, 2021. ; 9 function for every decade of older age, 2.7% lower function for every additional health condition, and 29% lower function for dual ChAdOx1 vaccination; all p<0.02) ( Table 2) . Among PLWH who were naive to COVID-19 at study entry, we observed a weak positive correlation between recent CD4 + T-cell count and ACE2 displacement activity after one dose that was not statistically significant (Spearman's ρ=0.18, p=0.09), and no association following two doses (Spearman's ρ=0.13, p=0.24; Figure 2C ). Similarly we observed a weak positive relationship between nadir CD4+ T-cell count and ACE2 displacement activity after one dose that was not statistically significant (Spearman's ρ=0.2, p=0.06), but no significant relationship after the second dose (Spearman's ρ=0.098, p=0.36) (Supplemental Figure 2B ). After one vaccine dose, plasma from most COVID-19 naive participants displayed weak or no ability to neutralize live SARS-CoV-2, with no significant differences between PLWH and controls (median/IQR undetectable in both groups; Mann-Whitney p=0.26) ( Figure 3A ). In contrast, neutralization activities in COVID-19 convalescent individuals were significantly higher, where reciprocal plasma dilutions needed to achieve neutralization were a median of 320 (IQR 80-320; p<0.0001 compared to both naive groups) ( Figure 3A) . Consistent with this, only COVID-19 convalescent status was significantly associated with higher neutralization activity in multivariable analyses after one dose ( Table 2) . Following two vaccine doses, viral neutralization activities in COVID-19 naive PLWH and controls increased an average of 8-fold in both groups (Supplemental Figures 1E, D) , with naive PLWH achieving neutralization at median reciprocal plasma dilution of 160 (IQR 40-320) compared to a median of 80 (IQR 40-160) in controls (p=0.08) ( Figure 3B) . The viral . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not certified by peer review) 10 neutralization activities of COVID-19 convalescent individuals (median reciprocal dilution of 160; IQR 140-640) remained marginally higher than COVID-19 naive individuals after two doses (p<0.02 for both comparisons). Consistent with the other humoral functions evaluated, multivariable analyses identified older age, a higher number of chronic conditions, and dual ChAdOx1 vaccination − but not HIV − as being independently associated with lower viral neutralization activity after two COVID-19 vaccine doses (p<0.02; Table 2 ). Among PLWH who were naive to COVID-19 at study entry, we observed a weak positive correlation between recent CD4 + T-cell count and viral neutralization activity after one dose (Spearman's ρ=0.21, p=0.04), but this association did not remain following two doses (Spearman's ρ=0.12, p=0.28; Figure 3C ). We observed no significant correlations between nadir CD4+ T-cell count and viral neutralization activity after either vaccine dose (Supplemental Figure 2C ). Given recent concerns that certain SARS-CoV-2 variants may be more transmissible or evade aspects of host immunity 44, 45 , we examined the ACE2 displacement activity in plasma against the widespread B.1.617.2 (Delta) variant. After one vaccine dose, plasma from all groups was impaired in its ability to block ACE2 receptor engagement by the Delta RBD compared to the original (Wuhan) RBD, where the magnitude of this impairment was a median of ~8%, ~19% and ~1% for COVID-19 naive PLWH, naive controls and convalescents, respectively (Wilcoxon matched pairs signed rank test, all p≤0.0001) ( Figure 4A ). After two vaccine doses, these impairments remained, albeit at a much lower magnitude (a median of ~2%, ~8% and ~1% for naive PLWH, naive controls and convalescents, respectively, all p<0.0001; Figure 4B ). Given . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not certified by peer review) the strong correlations between ACE2 displacement and viral neutralization activities observed in our study (Spearman's ρ≥0.58, p<0.0001; Supplemental Figure 3 ), these results suggest that vaccine-elicited humoral responses may be less able to prevent infection by the Delta variant, which is consistent with a recent report showing reduced ability of plasma from convalescent and vaccinated individuals to neutralize this strain 46 . Our results add to a growing body of evidence that adult PLWH receiving stable antiretroviral therapy, who have suppressed plasma HIV loads and who have CD4+ T-cell counts in a healthy range, generally mount robust humoral immune responses to COVID-19 vaccines 10, [33] [34] [35] . Though HIV infection was associated with marginally (0.2 log 10 ) lower overall anti-RBD antibody concentrations and ~11% lower ACE2 displacement activities following a single vaccine dose after adjustment for sociodemographic, health and vaccine-related variables, we observed no effects of HIV infection on anti-RBD antibody concentrations, ACE2 displacement or viral neutralization activities after two vaccine doses. Rather, older age and a higher burden of chronic health conditions were independently associated with weaker humoral responses after two vaccine doses, consistent with previous reports 37, [47] [48] [49] [50] . In addition, having received two ChAdOx1 doses, as opposed to a heterologous or autologous mRNA vaccine regimen, was associated with significantly lower "peak" humoral responses (measured one month following the second dose), which is also consistent with previous reports 51, 52 . Recent data however have indicated that, while humoral responses to the mRNA vaccines initially reach high levels but wane considerably thereafter, immune responses induced by a COVID-19 viral vectored vaccine induced lower median titers that remain more steady over time 53 . A longer interval between . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 15, 2021. ; 12 doses (where the maximum dose interval among study participants was 122 days) was also associated with marginally higher binding antibody concentrations, though not ACE2 displacement or viral neutralization activities, which is partially consistent with reports of improved antibody and T-cell responses using extended dosing intervals of the BNT162b2 mRNA vaccine 54 . Indeed, Canada's unique adoption of a very long (up to 112 days) interval between first and second COVID-19 vaccine doses yields insight into the magnitude of peak humoral responses following such an extended regimen. It is interesting that the anti-RBD antibody concentrations following two doses measured in the present study are generally higher than in studies of individuals who had shorter dose intervals that also employed the Roche Elecsys Anti-SARS-CoV-2 S assay, even though responses following dose 1 were similar [55] [56] [57] [58] . Comparing values across studies should be done with caution however, as the assay quantitative range will vary based on the maximum sample dilution performed. Importantly, among PLWH in our study, all of whom were receiving suppressive antiretroviral treatment, we observed only a very weak positive correlation between the most recent CD4+ T-cell count and humoral responses after the first vaccine dose. Moreover, this association disappeared following the second vaccine dose. While CD4+ T-cell counts <250 cells/mm 3 have been associated with lower antibody levels following one COVID-19 vaccine dose 36 , we were unable to confirm this finding as only two PLWH in the present study had CD4+ T-cell counts in this range, and both of them mounted strong vaccine responses. Moreover, although we found weak positive correlations between nadir CD4+ T-cell counts (which were as low as <10 cells/mm 3 in our cohort) and both anti-RBD antibody concentrations and ACE2 displacement activities following one dose, these associations no longer remained following the second dose. Furthermore, we observed no association between viral neutralization . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 15, 2021. ; https://doi.org/10.1101/2021.10.03.21264320 doi: medRxiv preprint 13 activity and nadir CD4 T+ cell count after either vaccine dose. This indicates that, for PLWH currently receiving suppressive antiretroviral therapy and whose CD4+ T-cell counts are currently in a healthy range, having had low CD4 T+ cell counts in the past will not necessarily compromise immune responses to COVID-19 vaccines presently. We also observed that the ability of vaccine-induced plasma antibodies to disrupt the ACE2/RBD interaction was modestly yet significantly reduced against the RBD of the now widespread SARS-CoV-2 Delta variant compared to the original strain for all participant groups. Given the ability of SARS-CoV-2 variants to evade at least some aspects of vaccine-elicited immunity 44 , this suggests that all individuals, regardless of HIV status, will remain more susceptible to infection by this variant, even after vaccination. Our study has several limitations. Our results may not be generalizable to PLWH who are not receiving antiretroviral therapy and/or who have CD4+ T-cell counts <200 cells/mm 3 . Our study did not include children or adolescents living with HIV. As the precise immune correlates of protection for SARS-CoV-2 transmission and disease severity remain incompletely characterized 59 , the implications of our results on individual-level protection from SARS-CoV-2 infection and COVID-19 remain uncertain. The relationship between vaccine-induced antibody concentrations in blood and at mucosal sites, which may be a better correlate of protection, is also incompletely understood, though a recent study identified anti-RBD IgG antibodies in saliva in 100% of participants following a two-dose COVID-19 vaccine series 60 . We did not investigate vaccine-induced T-cell responses, though two recent studies have demonstrated comparable anti-Spike T-cell responses in PLWH compared to controls 10, 34 . Our study was not designed to investigate potential differences in immune responses between the two mRNA . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. These results suggest that PLWH whose viral loads are well-controlled on antiretroviral therapy and whose CD4+ T-cell counts are in a healthy range will generally not require a third COVID-19 vaccine dose as part of their initial immunization series, though other factors such as older age, co-morbidities, type of initial vaccine regimen and durability of vaccine responses will influence when this group may benefit from additional doses. Further studies of PLWH who are not receiving antiretroviral treatment and/or who have low CD4+ T-cell counts are needed. . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. Health Care and Simon Fraser University Research Ethics Boards. All participants provided written informed consent. information were collected by self-report and confirmed through medical records where available. We assigned a score of 1 for each of the following 11 chronic health conditions: hypertension, diabetes, asthma, obesity (defined as having a body mass index ≥30), chronic diseases of lung, liver, kidney, heart or blood, cancer, and immunosuppression due to chronic . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 15, 2021. ; https://doi.org/10.1101/2021.10.03.21264320 doi: medRxiv preprint conditions or medication, to generate a total score ranging from 0-11. Clinical information for PLWH was recovered from the BC Centre for Excellence in HIV/AIDS Drug Treatment Program Database, which houses clinical records for all PLWH receiving care in BC. For PLWH, having a recent CD4+ T-cell count <200 cells/mm 3 was classified as "immunosuppression" in the chronic health conditions score. exhibiting only partial or no neutralization at 1/20 were coded as having a reciprocal dilution of "10", defined as below the limit of detection in this assay. Fisher's exact test. Comparisons of continuous variables between groups were performed using the Mann-Whitney U-test (for unpaired data) or Wilcoxon test (for paired data). Correlations between continuous variables were performed using Spearman's correlation. Multiple linear regression was employed to investigate the relationship between sociodemographic, health and vaccine-related variables and humoral outcomes. Analyses performed following one dose included age (per decade increment), sex at birth (female as reference group), ethnicity (nonwhite as reference group), number of chronic health conditions (per number increment), sampling date following vaccine dose (per day increment), and type of vaccine received (mRNA vaccine as reference group). Analyses performed following two doses additionally included the interval between doses (per week increment) and having received two ChAdOx1 doses (versus having received a heterologous or dual mRNA vaccine regimen). For assays that tested plasma . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 15, 2021. ; https://doi.org/10.1101/2021.10.03.21264320 doi: medRxiv preprint 18 (ACE2 displacement and viral neutralization), models also corrected for the anticoagulant used (ACD as the reference group). All tests were two-tailed, with p=0.05 considered statistically significant. Analyses were conducted using Prism v9.2.0 (GraphPad). . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 15, 2021. ; The authors have no competing interests to declare. . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 15, 2021. . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 15, 2021. ; https://doi.org/10.1101/2021.10.03.21264320 doi: medRxiv preprint are statistically significant are in bold ^ analyses performed on plasma (i.e. ACE2 displacement and viral neutralization) also correct for the anticoagulant used, with ACD as the reference category. Analyses of anti-RBD concentration do not correct for this variable because this assay was performed on serum. * for viral neutralization activity, reciprocal plasma dilutions were log 2 transformed prior to multivariable analysis, so estimates and 95% CI should be interpreted accordingly. c . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. 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Committee Statement Update on the use of COVID-19 vaccines in Persons Living with HIV Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine through 6 Months Efficacy and Safety of the mRNA-1273 SARS-CoV-2 Vaccine Safety and efficacy of the ChAdOx1 nCoV-19 vaccine (AZD1222) against SARS-CoV-2: an interim analysis of four randomised controlled trials in Brazil, South Africa, and the UK Safety and immunogenicity of the ChAdOx1 nCoV-19 (AZD1222) vaccine against SARS-CoV-2 in HIV infection: a single-arm substudy of a phase 2/3 clinical trial Safety and immunogenicity of the ChAdOx1 nCoV-19 (AZD1222) vaccine against SARS-CoV-2 in people living with and without HIV in South Africa: an interim analysis of a randomised, double-blind, placebo-controlled, phase 1B/2A trial BNT162b2 mRNA Covid-19 Vaccine in a Nationwide Mass Vaccination Setting Sustained Effectiveness of Pfizer-BioNTech and Moderna Vaccines Against COVID-19 Associated Hospitalizations Among Adults -United States Impact and effectiveness of mRNA BNT162b2 vaccine against SARS-CoV-2 infections and COVID-19 cases, hospitalisations, and deaths following a nationwide vaccination campaign in Israel: an observational study using national surveillance data Safety and Immunogenicity of Two RNA-Based Covid-19 Vaccine Candidates Durability of Responses after SARS-CoV-2 mRNA-1273 Immune responses to a single dose of the AZD1222/Covishield vaccine in health care workers Low immunogenicity to SARS-CoV-2 vaccination among liver transplant recipients Reduced humoral response to mRNA SARS-CoV-2 BNT162b2 vaccine in kidney transplant recipients without prior exposure to the virus Evaluation of Seropositivity Following BNT162b2 Messenger RNA Vaccination for SARS-CoV-2 in Patients Undergoing Treatment for Cancer Safety and immunogenicity of one versus two doses of the COVID-19 vaccine BNT162b2 for patients with cancer: interim analysis of a prospective observational study High seroconversion rate but low antibody titers after two injections of BNT162b2 (Pfizer-BioNTech) vaccine in patients treated with chemotherapy for solid cancers Humoral and cellular responses to mRNA vaccines against SARS-CoV-2 in patients with a history of CD20 B-cell-depleting therapy (RituxiVac): an investigator-initiated, single-centre, open-label study Effect of Immunosuppression on the Immunogenicity of mRNA Vaccines to SARS-CoV-2 : A Prospective Cohort Study Cellular and humoral immune responses following SARS-CoV-2 mRNA vaccination in patients with multiple sclerosis on anti-CD20 therapy Immunological benefits of antiretroviral therapy in very early stages of asymptomatic chronic HIV-1 infection Rapid restoration of CD4 T cell subsets in subjects receiving antiretroviral therapy during primary HIV-1 infection Immunological and virological responses in HIV-1-infected adults at early stage of established infection treated with highly active antiretroviral therapy Initiation of Antiretroviral Therapy in Early Asymptomatic HIV Infection Vaccination in the Adult Patient Infected with HIV: A Review of Vaccine Efficacy and Immunogenicity Long-term immune responses to vaccination in HIV-infected patients: a systematic review and meta-analysis Immunization for HIV-positive individuals Immunogenicity and safety of the BNT162b2 mRNA COVID-19 vaccine in people living with HIV-1 The BNT162b2 mRNA Vaccine Elicits Robust Humoral and Cellular Immune Responses in People Living with HIV Safety and antibody response to two-dose SARS-CoV-2 messenger RNA vaccination in persons with HIV Covid-19 vaccine immunogenicity in people living with HIV-1. bioRxiv, 2021 Factors That Influence the Immune Response to Vaccination National_Advisory_Committee_on_Immunization_(NACI). 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The variant gambit: COVID-19's next move Reduced sensitivity of SARS-CoV-2 variant Delta to antibody neutralization Targeting Inflammation and Immunosenescence to Improve Vaccine Responses in the Elderly Impact of Aging and Cytomegalovirus on Immunological Response to Influenza Vaccination and Infection Age-related immune response heterogeneity to SARS-CoV-2 vaccine BNT162b2 Age-dependent immune response to the Biontech/Pfizer BNT162b2 COVID-19 vaccination Heterologous ChAdOx1 nCoV-19 and mRNA-1273 Vaccination Immunogenicity and reactogenicity of heterologous ChAdOx1 nCoV-19/mRNA vaccination Differential Kinetics of Immune Responses Elicited by Covid-19 Vaccines Comparison of SARS-CoV-2 Antibody Response Following Vaccination With BNT162b2 and mRNA-1273 Initial SARS-CoV-2 vaccination response can predict booster response for BNT162b2 but not for AZD1222 Antibodies against severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) in individuals with and without COVID-19 vaccination: A method comparison of two different commercially available serological assays from the same manufacturer Evaluation of Three anti-SARS-CoV-2 Serologic Immunoassays for Post-Vaccine Response. The journal of applied laboratory medicine Correlates of protection against symptomatic and asymptomatic SARS-CoV-2 infection. medRxiv Antibody Responses to SARS-CoV-2 mRNA Vaccines Are Detectable in Saliva Effectiveness of Pfizer-BioNTech and Moderna Vaccines in Preventing SARS-CoV-2 Infection Among Nursing Home Residents Before and During Widespread Circulation of the SARS-CoV-2 B.1.617.2 (Delta) Variant -National Healthcare Safety Network Neutralizing antibody responses to SARS-CoV-2 variants in vaccinated Ontario long-term care home residents and workers. medRxiv Reduced magnitude and durability of humoral immune responses by COVID-19 mRNA vaccines among older adults. medRxiv We thank the leadership and staff of Providence Health Care for their support of this study. We thank the phlebotomists and laboratory staff at St. Paul's Hospital, the BC Centre for Excellence in HIV/AIDS and Simon Fraser University for assistance. Above all, we thank the participants, without whom this study would not have been possible.