key: cord-0852173-w7b2dfcd authors: Luo, Xiumei; Wu, Wenxian; Feng, Li; Treves, Haim; Ren, Maozhi title: Short Peptides Make a Big Difference: The Role of Botany-Derived AMPs in Disease Control and Protection of Human Health date: 2021-10-21 journal: Int J Mol Sci DOI: 10.3390/ijms222111363 sha: 032162b3b9554afdc2e423a042c883a641dd5cdd doc_id: 852173 cord_uid: w7b2dfcd Botany-derived antimicrobial peptides (BAMPs), a class of small, cysteine-rich peptides produced in plants, are an important component of the plant immune system. Both in vivo and in vitro experiments have demonstrated their powerful antimicrobial activity. Besides in plants, BAMPs have cross-kingdom applications in human health, with toxic and/or inhibitory effects against a variety of tumor cells and viruses. With their diverse molecular structures, broad-spectrum antimicrobial activity, multiple mechanisms of action, and low cytotoxicity, BAMPs provide ideal backbones for drug design, and are potential candidates for plant protection and disease treatment. Lots of original research has elucidated the properties and antimicrobial mechanisms of BAMPs, and characterized their surface receptors and in vivo targets in pathogens. In this paper, we review and introduce five kinds of representative BAMPs belonging to the pathogenesis-related protein family, dissect their antifungal, antiviral, and anticancer mechanisms, and forecast their prospects in agriculture and global human health. Through the deeper understanding of BAMPs, we provide novel insights for their applications in broad-spectrum and durable plant disease prevention and control, and an outlook on the use of BAMPs in anticancer and antiviral drug design. Pathogenic microorganisms pose a great threat to plants, animals, and humans. Global crop losses caused by bacteria, fungi, and viruses amount to USD 220 billion annually. Humans have also fought against pathogenic microorganisms throughout history. Catastrophic events such as smallpox, plague, and influenza change the course of human development time and time again. At present, we are still suffering from the devastating influence of SARS-CoV-2. During the "arms race" against pathogens, all organisms have evolved strategies to survive pathogenic infections. Among them, plants have developed unique, multi-level defense mechanisms against pathogens, including the use of physical barriers in the cell wall, induction of hypersensitive defense responses, expression of resistance proteins, and synthesis of antimicrobial peptides (AMPs) [1] . Botany-derived AMPs (BAMPs), a class of ubiquitous cationic polypeptides with less than 10 kDa of molecular weight, are the first line of defense in the non-specific innate immune system of plants [2] . cytoplasm after internalization into pathogen cells, targets mitochondria, inhibits the respiratory chain, produces ROS, and induces apoptosis, thus leading to pathogenic cell death [36] . Interestingly, defensins such as Psd1 from Pisum sativum can also enter the nucleus via nuclear migration to target and inhibit cyclin F, leading to cell cycle arrest and cell death [37] . The various interaction mechanisms between plant defensins and pathogens explain why these short peptides have contributed to innate plant immunity as potent antimicrobial molecules for thousands of years, and why they are attractive antimicrobial drug candidates for agricultural and clinical use. Although the mechanism by which plant defensins inhibit pathogen growth at the plasma membrane level through specific binding to sphingolipids (phospholipids) on the plasma membrane has been demonstrated and generally accepted, little is known about the internalization mechanism and the mode of action within pathogens. 6.15) . N and C represent N-terminus and C-terminus, respectively. Green represents the amino acid skeleton. Red represents cysteines and disulfide bonds between cysteines. PR1 is a small, secreted or vesicle-targeting antimicrobial protein specifically induced by salicylic acid (SA) [38] . The mature protein contains approximately six conserved cysteine residues, forming a tertiary α-β-α structure through three pairs of disulfide bonds ( Figure 1B) . As a marker protein for PTI and systemic acquired resistance (SAR), PR1 provides broad-spectrum resistance to a wide range of pathogens [39, 40] . PR1 is also found in yeast, insects, and vertebrates, including humans, which form the cysteine-rich 6.15) . N and C represent N-terminus and C-terminus, respectively. Green represents the amino acid skeleton. Red represents cysteines and disulfide bonds between cysteines. The various interaction mechanisms between plant defensins and pathogens explain why these short peptides have contributed to innate plant immunity as potent antimicrobial molecules for thousands of years, and why they are attractive antimicrobial drug candidates for agricultural and clinical use. Although the mechanism by which plant defensins inhibit pathogen growth at the plasma membrane level through specific binding to sphingolipids (phospholipids) on the plasma membrane has been demonstrated and generally accepted, little is known about the internalization mechanism and the mode of action within pathogens. PR1 is a small, secreted or vesicle-targeting antimicrobial protein specifically induced by salicylic acid (SA) [38] . The mature protein contains approximately six conserved cysteine residues, forming a tertiary α-β-α structure through three pairs of disulfide bonds ( Figure 1B) . As a marker protein for PTI and systemic acquired resistance (SAR), PR1 provides broad-spectrum resistance to a wide range of pathogens [39, 40] . PR1 is also found in yeast, insects, and vertebrates, including humans, which form the cysteine-rich secretory protein (CAP) superfamily, together with cysteine-rich secretory proteins (CRISPs) and antigen 5. Although PR1 is one of the most abundant proteins in the apoplast during pathogenic infection, little is known about its mechanism of action [18] . The role of PR1 in plant-pathogen interactions remains unclear. PR1a from tobacco was the first PR1 antimicrobial protein to be identified. Overexpression of PR1a significantly increases the tolerance of Nicotiana tabacum to tobacco downy mildew (Peronospora tabacina) and black shank (Phytophthora parasitica var. nicotianae) [41] . The synergistic overexpression of PR1a with P14c from tomato significantly inhibits the germination of Phytophthora infestans spores and suppresses its colonization in the host [42] . P. infestans is a sterol-auxotrophic pathogen that is highly sensitive to PR1 because PR1 sequesters sterols from the pathogen membrane, thus inhibiting pathogen growth [43] . Additionally, sterol-prototrophic pathogenic fungi exhibit high sensitivity to PR1 when their sterol biosynthesis is blocked, suggesting a positive correlation between the sterolbinding capacity of PR1 and its antifungal properties, and that the antimicrobial action of PR1 depends on the sterol synthesis ability of different microorganisms [43] . Further study demonstrated that PR1 bind sterols through its conserved CAP structural domain, which is essential for its antimicrobial activity and is conserved in the CAP superfamily and pathogen-related yeast (PRY) proteins [44] . In addition to sequestering sterols, PR1 can also inhibit programmed cell death upon pathogen infection [45] and induce the expression of host defense-related genes by releasing CAPE1 (CAP-derived peptide 1), a defense signal peptide. CAPE1, with the conserved motif PxGNxxxxxPY, originally derived from the last 11 amino acid residues of the Cterminus of tomato P14c, and is later cleaved at the C-terminus of PR1 and PR5 in a variety of plants [46, 47] . CAPE1 generates resistance to Pseudomonas syringae DC3000 and the larvae of Spodoptera litura by inducing the expression of defense-related genes, and induces immune responses against herbivores, pathogens, and abiotic stress [46, 47] . These data suggest that CAPE1 plays an important role in inducing host immune responses. Significant progress has been made in our understanding of PR1 function since CAPE1 peptides were identified and their roles in biotic stress responses were established. Moreover, an increasing number of studies have shown that PR1 promotes cell death by interacting with a variety of pathogen effectors [48] . For example, the ToxA effector of wheat pathogens Parastagonospora nodorum and Pyrenophora tritici-repentis interacts with the dimeric TaPR1-5 protein, leading to increased host cell necrosis [48] . Additionally, the P. nodorum effector SnTox3 interacts with multiple wheat PR1 family members [49] and prevents TaPR1 from releasing the TaCAPE1 peptide [40] . Furthermore, the important virulence factor SsCP1 in Sclerotinia sclerotiorum targets PR1 in Arabidopsis thaliana to inhibit its function, but SsCP1 can also be recognized by the plant, which triggers defense responses that lead to compatible interaction [50] . Interactions between PR1 and pathogen effectors suggest that when pathogens invade susceptible host plants, they secrete effectors to target PR1, thereby inhibiting its antimicrobial activity and achieving pathogen colonization and infection in the host. Conversely, resistant host plants express and secrete PR1 to target pathogen effectors or key virulence proteins associated with pathogenicity when invaded by pathogens, thereby achieving antimicrobial activity to control disease. Thionins are a class of small molecular weight (5 kDa), cysteine-rich, cytotoxic, basic BAMPs that belong to the PR13 family [51] . They contain 6 or 8 cysteines, and 3 or 4 disulfide bonds ( Figure 1C ). Originally isolated from cereals, approximately 100 thionins have since been identified in 15 monocotyledonous and dicotyledonous plant species [52] . Thionins are divided into two families with different structures, origins, and functions: α/β-thionins and γ-thionins. α/β-thionins are true PR13 family members, whereas γthionins are in common with defensins and belong to the PR12 family [53] . α/β-thionins have high sequence similarity with 45-48 amino acid residues and 3-4 disulfide bonds, and can be classified into types I-V [51] . Except for type IV thionins, which are neutral peptides, the other types of thionins are basic, with type V thionins consisting of a class of truncated peptides without cytotoxicity. The N-terminus of the thionin precursor protein contains a leading peptide of about 20 amino acid residues, while the C-terminus contains an acidic peptide of about 60 amino acid residues to neutralize the cationic bioactive peptide prior to its final maturation stage. Cleavage of the leading peptide is required for its toxic activity, and the mature domain is less conserved than the two flanking domains [54] . Thionins play an important role in the defense against pathogen invasion, with overexpression of thionin-encoding genes increasing disease resistance. For example, overexpression of endogenous Thi2.1 enhances the resistance of A. thaliana to F. oxysporum [55] , while heterologous overexpression of Thi2.1 from A. thaliana in tomato results in enhanced resistance to bacterial wilt and blight [56] . Further, high-level expression of the hordothionin gene from barley in tobacco confers resistance to Pseudomonas syringae [57] , while rice plants expressing the oat thionin gene display enhanced resistance to bacterial diseases [58] . Various studies have confirmed that thionins can act directly on cell membranes and exert antimicrobial activity by altering membrane permeability or forming ion channels [52] . Positively charged thionins have a strong electrostatic binding capacity to negatively charged phospholipids on the microbial membrane, which are naturally present as a protein-lipid complex [59] . In the resistance to pathogen invasion, thionins disrupt pathogenic membrane permeability in a dose-dependent manner, with a critical dose (approximately 1 µM) directly associated with antimicrobial activity and membrane lysis, and an approximately 1 h duration of activity [60] . In addition, thionins interact with lipid transfer proteins (LTPs) to exert synergistic antimicrobial activity, suggesting that these proteins may cooperate in membrane binding and/or permeation [52, 61] . Besides cytotoxicity, thionins are involved in other cellular processes. Experiments performed on artificial cell membranes and different cell lines demonstrated that thionin treatment depolarizes membranes, causes cell lysis, and increases Ca 2+ channel permeability [62] . After binding to calmodulin, thionins activate endogenous phospholipase A2 (PLA2) and adenylate cyclase, inhibit protein kinase C, and suppress DNA and protein synthesis in cell-free systems [52] . Furthermore, thionins possess thioredoxin activity and are involved in redox regulation of enzymes as a secondary messenger [62] . These events following cell membrane disruption amplify the initial toxic effects of thionins and disrupt many key cellular processes, ultimately leading to cell death [63] . Overall, thionins directly target negatively charged phospholipids in the cell membrane rather than specific protein receptors on the cell surface [52] . Any membrane containing neutral or moderately cationic phospholipids can also be disrupted by thionins [64] . LTPs are a class of multigene-encoded, abundant, soluble, and structurally compact cationic small peptides [65] . As the name implies, the primary function of LTPs is to facilitate the transfer of various types of lipids, including phosphatidylinositol, phosphatidylcholine, and galactolipids [66] . With their low specificity for lipid substrates, plant LTPs are also known as nonspecific lipid transfer proteins (nLTPs). Their protein structure contains four conserved disulfide bonds, and 4-5 α-helices fold to form a tight, heat-and denaturant-insensitive three-dimensional structure ( Figure 1D ). Hydrophobic cavities are formed to facilitate lipid binding and transport [67] . Based on the spacing between cysteine residues, sequence differences, and post-translational modifications, LTPs are divided into five major classes (LTP1, LTP2, LTPc, LTPd, LTPg) and five minor classes (LTPe, LTPf, LTPh, LTPj, LTPK) [68] . Among the major classes, LTPd and LTPg are present in all terrestrial plants, suggesting that they may be the earliest evolved LTPs, whereas LTP1 and LTP2, the most well-studied LTPs in flowering plants, likely evolved later and are not found in algae, mosses, or other non-seed plants [68] . The N-terminus of the LTP precursor contains a signal peptide for the cellular secretion pathway that locates LTPs in the intercellular space outside the plasma membrane [69] . LTPs have a variety of biological activities, such as promoting cell expansion and plant growth [70] , participating in lipid metabolism [71] , and being responsible for wax and lipid barrier polymer deposition [72] [73] [74] . In addition, LTPs are an important part of plant defense, with their encoding genes being abundantly expressed in response to pathogen infection. Furthermore, transgenic overexpression of LTP genes enhances host tolerance to pathogen infection; thus, LTPs are classified as members of the PR14 protein family. Homologous overexpression of LTP genes and heterologous overexpression of barley LTP genes in A. thaliana enhance plant tolerance to P. syringae and Botrytis cinerea [75] , while the expression of barley LTP genes in tobacco also enhances its resistance to P. syringae [76] . CaLTP1 isolated from Capsicum annuum seeds exerts antimicrobial activity against Saccharomyces cerevisiae, Pseudomonas tropicalis, and Colletotrichum lindemuthianum, causing morphological damage by penetrating the plasma, and leading to pseudo-hyphae formation [77] . All four nsLTP homologous peptides (CW18-21) isolated from barley and maize can inhibit Clavibacter michiganensis, P. solanacearum, and F. solani [61] . Other LTPs, such as Ha-AP10 [78] , Ace-AMP1 [79] , and NTP [61, 80] , can inhibit the growth, development, and pathogenicity of pathogenic fungi and bacteria to varying degrees. LTPs in plants exhibit broad-spectrum antimicrobial activity by inhibiting the growth of pathogens, and exhibit low toxicity to plant and mammalian cells [81] . However, little has been reported on their antimicrobial mechanism. It has been hypothesized that LTPs may interact with lipids in microbial membranes, causing the lipids to translocate to the extracellular compartment, thus leading to membrane permeation or apoptosis [82] . The detailed mechanism of action can be found in the model of LTPs associated with carcinogenesis (see Section 3.3). PIs, which belong to the PR6 family, are a subclass of tomato/potato inhibitor I-related serine PIs, with a molecular weight of 8 kDa and 4 disulfide bonds ( Figure 1E ) [83, 84] . Since all types of PIs can interact with pathogenic proteases to exert host defense functions, limiting PIs to serine protease inhibitors is controversial [85, 86] . PIs have multiple biological functions, including regulation of endogenous proteases during seed dormancy, mobilization of protein reserves [86] , and host defense [87] . During disease defense, PIs can reduce pathogen aggressiveness by inhibiting the lyase activity required for fungal pathogenicity [88] , blocking the replication cycle of viruses [89] , and inhibiting the digestive enzyme activity of nematodes and insects, thereby limiting the release of amino acids [90] . Both fungal and bacterial infections can induce substantial expression of PI genes. For example, P. infestans [91] and P. syringae [92] induce the expression of PI genes in tomato. In vitro experiments have shown that barley trypsin PI could inhibit Alternaria brassicola, Ascochyta pisi, F. culmorum, and Verticillium dahliae [93] , and the activity is synergistically enhanced when combined with thionin (PR13). In addition, HyPep, a serine PI isolated from C. annuum seeds, is able to completely inhibit the growth of S. cerevisiae and C. tropicalis at 25 mg/mL, leading to cell aggregation and pseudo-hyphae formation [94] . Buckwheat trypsin PI is able to inhibit the protease activity necessary for the pathogenicity of B. cinerea in vitro [88, 95] . Lorito et al. [96] concluded that PIs can inhibit fungal growth by inhibiting endogenous trypsin that is essential for chitin synthase, thus blocking chitin synthesis in fungal cell walls [97] . Since the role of specific microbial proteases in pathogenicity is unclear, the effects of plant PIs on the activity of these enzymes need further study. Table 1 . Main families of botany-derived antimicrobial peptides (BAMPs) and their modes of action. Representative Peptide Sources Mode of Action References Dahlia merkii DmAMP1 binds to M(IP)2C in the membrane, resulting in potassium efflux, calcium uptake, membrane permeability change, and CWI pathway activation. [25, 28, 29] RsAFP2 RsAFP2 binds to GlcCer in the cell wall and plasma membrane, resulting in ROS production, apoptosis, ion fluxes, and CWI pathway activation. [30, 31] Family Medicago sativa MsDef1 interacts with GlcCer in the cell wall and membrane, resulting in the activation of MAPK cascade in the CWI pathway, and disruption of Ca 2+ signaling and homeostasis, contributing to fungal cell death. [32, 33, 98] MtDef4 MtDef4 binds to PA and is internalized into the fungal cell, resulting in the disruption of Ca 2+ signaling and homeostasis in a different way to MsDef1. An interaction with unknown intracellular targets has been proposed. [34] Nicotiana alata NaD1 binds to PI(4,5)P2 and dimerizes in the membrane. The dimer is internalized into the cytoplasm and interacts with intracellular targets to trigger the ROS and NO production. [33, 35] HsAFP1 HsAFP1 binds to the fungal cell wall and plasma membrane via loop 5. It moves into the cytoplasm and targets mitochondria, produces ROS, and induces programmed cell death. [36] Pisum sativum Psd1 binds to GlcCer in the plasma membrane. It moves to the cytoplasm and interacts with cyclin F in the nucleus, which results in cell cycle arrest and fungal cell death. Nicotiana tabacum PR1 binds to sterol and sequesters it from pathogens. It inhibits programmed cell death at the pathogen infection sites, and induces the expression of host defense genes by releasing CAPE1 peptide. Solanum lycopersicum Upon application of purothionin, there is a depolarization of the membrane and Ca 2+ ion permeability increases. β-purothionin interacts with dimyristoyl-phosphatidylglycerol, and inhibits protein kinase C. [59, 99] Thionin Thionin leads to membrane depolarization, influx of exogenous Ca 2+ , and activation of PLA2 and adenylate cyclase. [62] viscotoxins A3 viscotoxins B Viscum album Viscotoxins directly interact with DNA and RNA, interfering with nucleic acid synthesis. [100] Lipid transfer proteins (LTPs, PR14) Capsicum annuum Ca-LTP1 penetrates the plasma membrane and causes morphological damage, accompanied by pseudo-mycelia formation. [77] Ha-AP10 Helianthus annuus Ha-AP10 interacts with phospholipids and produces a direct cytotoxic effect on fungal cells mediated by membrane permeabilization. [78] Proteinase inhibitor (PIs, PR6) HyPep Capsicum annuum HyPep inhibits α-amylase and serine proteinases, and causes cell aggregation and pseudo-mycelia formation. [94] In addition to defending against plant disease, BAMPs are also potential candidates for the treatment of human diseases. Here, we mainly focus on the antifungal, antiviral, and anticancer activity of BAMPs in plants and humans. Pathogenic fungi are considered a greater threat to plant and animal biodiversity than other taxonomic classes [101] . As part of the intrinsic immune system, BAMPs have great potential to be developed as novel antifungal agents due to their broad-spectrum activity, selective targeting, multiple mechanisms of action, and limited cell cytotoxicity [7, 102] . Elucidating the functional mechanism of BAMPs is key to uncovering their application potential and developing new therapeutic approaches. The mechanisms underlying the antifungal action of BAMPs can be grouped into the following categories ( Figure 2A ): (1) BAMPs interact with the fungal cell surface. Through electrostatic action, BAMPs adsorb onto the membrane surface of pathogens, and form a central lumen in the membrane through barrel-stave, carpet, or toroidal pore mechanisms, which induces lipid bending and eventually leads to pore formation [7] . (2) BAMPs bind to the components on the fungal cell membrane. The cell membrane is mainly composed of sterols, phospholipids, and sphingolipids. Based on the polar head groups, phospholipids can be further divided into PA, phosphatidylcholine, phosphatidylethanolamine (PE), phosphatidylglycerol, phosphatidylserine (PS), and inosinephosphatidylinositides, while sphingolipids are divided into sphingomyelin and glycosphingolipids. Many BAMPs can interact with cell membrane components, such as phospholipids and sphingolipids. For example, NaD1 kills fungal cells by binding to PA via the 39th arginine [103] . GlcCer, the most common glycosphingolipid in fungi [104, 105] , also plays an important role in the antifungal activity of BAMPs, including MsDef1, Psd1, Psd2, PvD1, Sd5, and RsAFP2. Interestingly, although most sphingolipids are not essential for cell survival, they are key regulators of the pathogenicity of various fungi and are essential components needed for infection in vivo [106] . (3) BAMPs interact with the fungal cell wall. Fungal cell walls are mainly composed of glucan, chitin, and glycosylated proteins [107] . GlcCer is also an important component of fungal cell walls [108] . A variety of BAMPs are able to bind to major cell wall components, such as RsAFP2 targeting GlcCer, and hevein-like peptides binding chitin, thereby inhibiting cell wall formation and pathogenic fungal growth [109] . Notably, BAMPs and human-derived AMPs such as histatin 5 and neutrophil defensin 1 (HNP-1) have a similar mechanism of inhibiting cell wall biosynthesis [110] . (4) BAMPs act on intracellular targets and participate in cellular signaling pathways. The antifungal mode of BAMPs in vivo mainly includes the induction of endogenous ROS production and programmed cell death, mitochondrial dysfunction, adenosine triphosphate (ATP) efflux, cell cycle disorder, cation homeostasis disruption, autophagy induction, and vesicular dysfunction [7] . BAMPs such as HsAFP1 and NaD1 are capable of internalization within the fungal cytoplasm through endocytosis to exert their antifungal effects [7] . Notably, the internalization process of BAMPs is often species-specific. For example, OefDef1.1 transfers to the cytoplasm at the plant germling and pathogenic conidia stages in F. oxysporum, whereas the internalization only occurs at the germ development stage in B. cinerea [111] . Furthermore, BAMP internalization is not indispensable for the induction of intracellular mechanisms. For example, RsAFP2 does not need to be internalized within C. albicans to induce ROS production and programmed cell death [31] . BAMPs can control viral infection by disrupting viral envelope structures, blocking the interaction of viruses with host cells, and inhibiting viral replication (Table 2) . StPIP1, a pathogen-associated molecular pattern (PAMP)-inducible peptide in potato, triggers plant defense responses against potato Y virus (PVY) through inducing ROS production, callose deposition, and defense-related gene expression during compatible interaction with PVY [112] . Peptides A22 and A64 interfere with viral replication by binding to the origin of replication loop structure (OriRep) of tomato golden mosaic virus (TGMV) [113] . Co-expression of these two peptides in tomato plants infected with tomato yellow leaf curl virus (TYLCV) or tobacco mottle virus (ToMoV) effectively reduces the disease symptoms [113] . Consistent with the action mechanism of A22 and A64, the AmPep1 peptide obtained from the globulin of Amaranthus hypochondriacus seed highly binds to the OriRep of TYLCV and pepper yellow vein virus (PHYVV), inhibiting viral replication and alleviating disease symptoms in Nicotiana benthamiana [114] . This was the first report of direct exogenous expression of a peptide for controlling plant DNA virus. Since then, Rudolph et al. [115] BAMPs can control viral infection by disrupting viral envelope structures, blocking the interaction of viruses with host cells, and inhibiting viral replication (Table 2) . StPIP1, a pathogen-associated molecular pattern (PAMP)-inducible peptide in potato, triggers plant defense responses against potato Y virus (PVY) through inducing ROS production, callose deposition, and defense-related gene expression during compatible interaction with PVY [112] . Peptides A22 and A64 interfere with viral replication by binding to the Meliacine prevents the hulling process of FMDV by inhibiting vacuolar acidification, and thus restrains the virus proliferation. [120] Pep-RTYM binds to the DENV particles to prevent viral interaction with host cell receptors and the subsequent nucleic acid release. [121, 122] Plants are also an excellent source of antiviral peptides for human viruses. For example, a 9 kDa nonspecific LTP (NTP) isolated from Narcissus tazetta var. chinensis L. inhibits the proliferation of influenza A (H1N1) virus by blocking the ceramidase on the viral envelope [16] . Further, NTP prevents respiratory syncytial virus (RSV) from entering host cells and interferes with RSV transmission by binding to viral glycoproteins or inhibiting other events in viral replication or assembly [16] . Moreover, ginkbilobin isolated from ginkgo seeds, ascalin from Allium ascalonicum bulbs, lunatusin from Phaseolus lunatus L. seeds, and vulgarinin from Phaseolus vulgaris seeds can inhibit HIV-1 proliferation by inhibiting HIV-1 reverse transcriptase activity [116] [117] [118] [119] . Meliacine (MA), a peptide isolated from the leaves of the Melia azedarach L., inhibits the proliferation of foot-andmouth disease virus (FMDV) in BHK-21 cells by blocking the uncoating process of the virus through inhibiting vesicular acidification [120] . Pep-RTYM, a novel active peptide isolated from the Asian medicinal plant Acacia catechu, demonstrates broad antiviral activity against four serotypes of dengue virus (DENV) in the early stages of viral infection [121, 122] . By binding to DENV particles, Pep-RTYM prevents interaction of the virus with cellular receptors and the subsequent release of nucleic acids, without apparent cytotoxicity [122] . Besides BAMPs, some proteins also function in plant antiviral resistance and have shown great potential for plant protection applications. JAX1, a jacalin-type lignan-like lectin protein identified from Bay-0 ecotype A. thaliana, significantly increases the plant's resistance to potato X virus (Potexvirus), and was further found to interact with the RNAdependent RNA polymerase (RdRp) of Potexvirus, inhibiting its replication activity and thus blocking viral infection [123, 124] . Beclin1 (ATG6) is an autophagy core protein that acts as a selective autophagy receptor, targeting viral replicase (a nuclear inclusion 'b' protein, NIb) and mediating ATG8a-dependent selective autophagy [125] . Additionally, Beclin1 is able to interact with viral NIb and inhibit its RdRp activity in an autophagy-independent manner [125] . Cyclophilin Cpr7p, a molecular chaperone in plants and animals, strongly inhibits viral RNA recruitment, the assembly of viral replicase complexes, and viral RNA synthesis during tomato bushy stunt virus (TBSV) replication [126] . Using the active regions of these antiviral proteins to prepare specific antiviral peptides would provide new resources for broad-spectrum disease control. Collectively, antiviral BAMPs may control viral infection and proliferation by ( Figure 2B) : (1) Disrupting the viral envelope, resulting in membrane destabilization and disruption, thus inhibiting the virus' ability to infect host cells [127] [128] [129] . (2) Blocking viral binding to host cells by competitively binding to viral capsid proteins or host cell surface receptors, thereby preventing viral uncoating and genome release into host cells [130] [131] [132] [133] [134] [135] . (3) Crossing the host cell membrane into the cytoplasm or nucleus to elicit the host defense system against the virus or regulating cellular pathways to block viral gene expression, thereby inhibiting viral replication [118, 119, 136] . (4) Targeting structural proteins to inhibit the assembly of viral particles, enhance host phagocytosis, etc., thereby preventing viral replication and transmission [127] . Integrating BAMPs resistance against plant and animal viruses and exploring their target proteins will provide new ideas and approaches for the development of antiviral peptide drugs for agricultural and medical use. BAMPs are important members of the anticancer drug family ( Table 3 ). The most well-known anticancer BAMP is lunasin, a small peptide consisting of 43 amino acid residues from the 2S albumin in soybean seed, which contains three functional domains: the chromosome-targeting domain, the cell adhesion (Arg-Gly-Asp, RGD) domain, and the cysteine tail that binds histones H3 and H4. By targeting cellular chromatin, this small peptide acts on the highly basic region of the N-terminal end of histones in the centromere, disrupting the normal formation of the mitotic complex, blocking normal mitosis, and ultimately leading to cancer cell death [137] . Now, lunasin has been identified in a variety of plants, including barley, wheat, loblolly, quinoa, and oats [138, 139] . In addition, cycloviolacin O2 (CyO2), a cyclic peptide isolated from Viola odorata, is a promising anticancer drug that causes necrosis of human lymphoma cells by disrupting cell membranes and is selectively toxic to tumor cells relative to normal cells [140] . The cyclic peptide MCo-PMI, obtained by engineering processing MCoTI-I, inhibits prostate tumor growth by activating the p53 tumor suppressor pathway [141] . The cyclic peptide HB7 from Hedyotis biflora significantly inhibits tumor proliferation and migration in an in vivo xenograft model [142] . The action mechanism of these cyclic peptides may be related to their ability to target and disrupt cell membranes; therefore, a better understanding of the membrane specificity of cancer cells will help design novel drugs based on the cyclic peptide framework, and allow specific peptide drugs to target different cell types. Additionally, NaD1 has been shown to inhibit the proliferation of monocytic lymphoma through direct binding to the phosphatidylinositol 4,5-bisphosphate (PIP2) on the plasma membrane [143] . Viscotoxin B2 inhibits rat osteogenic sarcoma through membrane lysis [144] , and ligatoxin B inhibits the proliferation of lymphoma and adenocarcinoma cells through inhibiting nucleic acid and protein synthesis [145] . Relative to other BAMPs, increasing evidence supports that LTPs play an important role in tumor progression and metastasis. Phosphatidylinositol, sphingolipids, and fatty acids act as second messengers in key signaling pathways that control cell survival, proliferation, and migration. LTPs such as NTP [16] mediate cancer-associated signaling cascades by regulating the distribution of lipids within the cell membrane, thereby inhibiting tumor cell infiltration and metastasis [146] . There are also many other antitumor BAMPs of which the action mechanisms are unclear, such as phoratoxins C-F [147] , Thi2.1 [148] , sesquin [149] , limenin [150] , and coccinin [151] . Besides BAMPs, other small peptides demonstrate anticancer activity in plants (Table 3) . For example, Cn-AMP1 derived from Cocos nucifera can reduce cancer cell viability without causing hemolysis [152] and Cr-ACP isolated from Cycas revoluta arrests the Hep2 cell cycle in the G0-G1 phase [153] . Further, the cyclic heptapeptide cherimolacyclopeptide C isolated from Annona cherimola seeds displays in vitro cytotoxicity against KB cells [154] , while a cell cycle-inhibiting octapeptide cyclosaplin purified from Santalum album L. inhibits breast cancer cell proliferation in a dose-and time-dependent manner [155] . Moreover, the cyclic peptides Poca A and B isolated from Pombalia calceolaria roots can inhibit breast cancer cell migration, which are inactive at less than 1 µM, and are toxic at higher concentrations [156] . IbACP, a small peptide of 16 amino acid residues obtained from the leaves of sweet potato, is able to rapidly alkalinize cell tissues and induce apoptosis in tumor cells through a mitochondria-dependent pathway [157] . Taken together, the above studies indicate that BAMPs can kill cancer cells by membrane lysis ( Figure 2C ) [158] . Owing to the composition of phosphatidylserine, O-glycosylated mucin, sialylated gangliosides, and heparan sulfate on the membrane surface of cancer cells, the membrane has a negative charge, which is in contrast to normal mammalian cell membranes [159] . Thus, positively charged BAMPs can selectively target cancer cells. However, the non-membrane solubilizing activity of BAMPs is involved in regulating processes such as angiogenesis, apoptosis, autophagy, and cell cycle that are critical for tumor proliferation and migration ( Figure 1C ) [160] . Normal and tumor cells differ in many ways, such as the presence of specific receptors such as integrin αvβx, aminopeptidase APN, peptide transporter protein PEPT1, and epidermal growth factor receptor EGFR on the surface of cancer cells. In addition to interfering with membrane permeability through electrostatic effects, do BAMPs bind specifically to cancer cell surface receptors and kill cancer cells by inducing host immune responses or by entering intracellular to target signaling pathways? Exogenous proteins have been shown to enter cells by integrin receptor-mediated means [161] . Therefore, ideas for novel anticancer drugs may be obtained by screening AMPs in medicinally valuable plants, animals, and microorganisms focusing on cancer cell surface receptors. Table 3 . Representative botany-derived antimicrobial peptides (BAMPs) and some other small peptides with anticancer activity and their modes of action. Representative Peptide Anticancer Activity BAMPs Lunasin Skin, colon, prostate, and breast cancers Lunasin binds directly to deacetylated histones, inhibits acetylation, and turns off the transcription. [137] Cycloviolacin O2 (CyO2) Breast cancer and lymphoma cells CyO2 causes tumor cell death by membrane permeabilization. [140] MCo-PMI Adenocarcinoma MCo-PMI inhibits tumor proliferation by activating the p53 tumor suppressor pathway. [141] HB7 Pancreatic cancer HB7 inhibits the proliferation and migration of tumors by membrane permeabilization. [142] NaD1 Monocyte lymphoma NaD1 inhibits the proliferation of monocyte lymphoma by directly binding to the plasma membrane phosphatidylinositol 4,5-diphosphate. [143] Viscotoxin B2 Osteogenic sarcoma Viscotoxin B2 inhibits tumor cells by membrane lysis. [144] Ligatoxin B Lymphoma and adenocarcinoma Ligatoxin B inhibits the proliferation of tumor cells by inhibiting nucleic acid and protein synthesis. [145] NTP mediates cancer-related signal transduction cascades by regulating the distribution of lipids in cell membranes, thereby inhibiting tumor cell invasion and metastasis. [16, 146] Phoratoxins C-F Different types of solid tumor cells and hematologic tumors Inhibiting tumor proliferation, while the mechanism of action is unknown. [147] Thi2.1 Tumor cells McF-7, A549, and HeLa [148] Sesquin McF-7 and leukemia M1 cells [149] Limenin Leukemia cells [150] Purple pole defensin HepG2, McF-7, and HT-29 cells [162] Coccinin HL60 and L1210 cells [151] Other small peptides Cn-AMP1 Caco-2 cells Cn-AMP1 reduces cancer cell viability without causing hemolysis. [152] Cr-ACP Hep2 cells Cr-ACP induces cell cycle arrest in G0-G1 phase. [153] Cherimolacyclopeptide C KB cells Cherimolacyclopeptide C shows in vitro cytotoxicity to KB cells. [154] Cyclosaplin Breast cancer Cyclosaplin inhibits cancer cell proliferation in a dose-and time-dependent manner. [155] Poca A and B Breast cancer Poca inhibits cancer cell migration. [156] IbACP Panc-1, a pancreatic cancer line IbACP regulates cellular proliferation by inducing and promoting apoptosis through the mitochondrial apoptotic pathway. [157] GLTSK HCT116, human colorectal cancer cells GLTSK decreases angiotensin II-dependent proliferation in HCT116 through the blockade of the renin-angiotensin system. [163] The in vitro and in vivo activities of many BAMPs are well-known and show clear potential for agricultural purposes. Plants expressing exogenous BAMP variants, synthetic BAMPs, and isogenous BAMPs are capable of producing resistance to a variety of pathogens [164, 165] . Particularly, defensins have been heterologously expressed in many economically important crops to enhance resistance to pathogenic fungi. The radish defensin Rs-AFP2 was the first heterologous BAMP to be expressed in other plants, inducing protection against Alternaria longipes in tobacco and tomato [13] , and against different fungi in wheat [14] . Other BAMPs also have been used to induce resistance in different plants, such as overexpression of Pn-AMP in tobacco and tomato enhances their resistance to Phytophthora nicotianae and Phytophthora capsici, respectively [166] . Further, overexpression of the barley LTP gene in A. thaliana enhances its resistance to Pseudomonas syringae and Staphylococcus griseus [167] . Compared with resistance enhancement by overexpression of a single gene, co-expression of multiple genes could incur stronger resistance. For example, co-expression of the defensins Dm-AMP1 and Rs-AFP2 in rice results in plants with stronger antifungal activity than the expression of either defensin alone [168] . In addition to their antimicrobial activity, BAMPs are able to promote plant growth and optimize crop traits. Therefore, combining their antimicrobial ability with growth-promoting effects can better control disease, improve crop yield, and ensure food security. Additionally, many non-plant AMPs also play an important role in plant protection in agriculture. For example, cecropin A and B from Hyalophora Cecropia are expressed in rice and tomato, enhancing the resistance of these crops to bacterial and fungal diseases [169] [170] [171] . The undecapeptide BP100 and its derivatives, which were identified from a library of synthetic cecropin A-melittin hybrids, can also improve the anti-pathogen activity of plants [172, 173] . Unfortunately, due to their limited stability and activity conditions, BAMPs and non-plant AMPs have not yet been directly prepared as commercial biofungicides. The rational, efficient, and innovative use of BAMPs will have a multiplier effect on the disease prevention and control. In summary, the following strategies are proposed ( Figure 3A ): (1) Exploring the expression regulation mode of BAMPs and optimizing BAMPs codons to increase their expression and activity. (2) Excluding the toxic effects of BAMP accumulation on host plants by using pathogens to induce high expression of BAMPs with high activity, multiple mechanisms of action, and low toxicity. (3) Inducing synergistic expression of BAMPs from different sources, with variable mechanisms of action to expand the range and variability of host resistance to pathogens. (4) Modifying BAMPs in vitro to enhance their stability and improve disease resistance for preparing them into biological agents for direct external application. Overall, the use of BAMPs for both molecular breeding and direct external application can reduce the use of chemical pesticides, control crop losses, and ensure food security. Combining multiple biotechnologies can better expand their application potential. and multigene-induced expression (c) can elevate BAMPs expression and broaden host resistance to pathogens. Furthermore, modification of BAMPs in vitro, such as cyclization, can enhance their stability and promote antimicrobial activity, which can be directly used as a biological agent in the future (d). (B) The application of BAMPs for human health. Using fresh-edible plants or chlorella as a bioreactor to prepare oral BAMPs drugs can not only break through the restriction of intravenous injection, but also greatly increase the yield and decrease the cost (e). On the one hand, BAMPs can be biosynthesized in organelles such as vacuoles and chloroplasts to protect them from being degraded by digestive enzymes during oral administration. On the other hand, BAMPs can be directly secreted in the fermentation broth and be prepared for intravenous injection when chlorella is used as a bioreactor. Additionally, N-and/or C-terminus modification of BAMPs can increase their stability and their ability to pass through the biological membrane (f). BAMPs have emerged as important novel candidates for the treatment of various human infections due to their high efficiency, specific selectivity, broad range of targets, high permeability in tissues, low immunogenicity, low toxicity, and tissue accumulation. In addition to antimicrobial effects, some BAMPs have shown promise as anticancer agents, as their activity against target cells and/or induced immune responses can effectively control infections and reduce tumorigenesis [10] . Further, BAMPs have shown promising therapeutic effects in combination with conventional therapies [10] . The most well-studied BAMP, lunasin [137] , has become the reference and standard for elucidating the anticancer mechanism of BAMPs, and provides a viable candidate for drug development. In addition, the reported anticancer mechanisms of BAMPs, such as viscotoxin B2 [144] , ligatoxin B [145] , NaD1 [143] , CyO2 [140] , MCo-PMI [141] , and NTP [16] , have indicated their potential to be developed as anticancer drugs. The numerous advantages of BAMPs have successfully attracted the attention of the pharmaceutical industry [174] . Currently, some BAMPs and their derivatives are in various clinical trial stages, such as Brilacidin (defensin mimetic), Surotomycin (cyclic lipopeptide), PAC-113 (12 amino acid antimicrobial peptide), and HB1275 (lipohexapeptide) [175] . Like other peptides of natural origin, BAMPs mostly exist at very low concentrations, and would need to be produced in large quantities for pharmaceutical or biological applications [10] . Although the development process is similar to that of traditional small molecule drugs, peptide drugs require unique process designs, preparation methods, structure confirmation, and production equipment. In particular, the large-scale commercial production of high-purity antimicrobial peptides is a practical demand for the treatment of diseases. The lack of a suitable manufacturing platform in terms of product yield, cost, and purity is a barrier to the medical use of BAMPs. Recent advances in biotechnology allow plants to be employed as bioreactors for BAMPs production, as mentioned above [176] . In addition, non-plant AMPs can also be expressed in plants for their use in a global health strategy. For example, retrocyclin-101 (RC101) and protegrin-1 (PG1) are two important antimicrobial peptides that can be used to treat bacterial and/or viral infections, especially those caused by HIV-1 or sexually transmitted bacteria. Lee et al. used plant molecular farming to achieve stable expression of these two AMPs in tobacco, which accounted for 38% and 26% of total soluble protein of chloroplast, respectively [177] . Similarly, lactostatin is an anti-hypercholesterolemic peptide derived from β-lactoglobulin in cow's milk. Cabanos et al. realized that the expression of lactostatin in rice, and its content in dry seeds, reached 2 mg/g, which has potential clinical application value as an anti-high-cholesterol peptide drug [178] . Therefore, the establishment of transgenic plants expressing bioactive BAMPs and non-plant AMPs is a promising strategy for the production of therapeutic AMPs. In addition, peptides have a short half-life and are easily broken down by enzymes in the body, making them difficult to absorb through the digestive system. Accordingly, peptide drugs are often administered by injection. Based on the limitations of peptide drug production and clinical delivery methods, the following solutions are proposed ( Figure 3B ): (1) Prioritizing cyclic peptides or cyclizing linear peptides with high antimicrobial activity and anticancer activity, since the head-to-tail cyclic backbone and the cysteine binding motif of cyclic peptides make them more stable and resistant to thermal or enzymatic degradation [179] . Additionally, the cyclization of linear peptides leads to a reduction in their conformational flexibility or actually decreases the number of hydrogen bonds formed by the peptide, thus increasing their ability to pass through biological membranes and their resistance to endopeptidases and exopeptidases [180] . (2) Using fresh-edible plants as bioreactors and BAMPs with medicinal value as a starting point, then, modify the N-terminus of BAMPs by compartmentalization and localization to allow targeted biosynthesis of BAMPs in organelles such as vesicles and chloroplasts, thereby adding a protective layer and reducing their degradation by the digestive tract during consumption. (3) Combined with synthetic biology, scaling up production of medicinal BAMPs using Chlorella pyrenoidosa as a bioreactor, which could be photoautotrophic and heterotrophic. C. pyrenoidosa is a single-celled algae that is rich in nutrients and protein content (more than 50% of dry weight), making it a new resource food with health value. Due to its fast growth rate, simple nutritional requirements, low cost, and that it can be cultivated via heterotrophic fermentation on a large scale, C. pyrenoidosa is an ideal chassis for biosynthesis. On the one hand, using C. pyrenoidosa as a chassis to synthesize BAMPs can directly treat diseases through oral administration. On the other hand, BAMPs could be synthesized and secreted outside the cells, enabling extraction from the fermentation broth and subsequent modification to enhance their stability for oral or intravenous administration. (4) Modifying the N-and/or C-terminus of the peptide sequence through N-acylation, N-esterification, or C-amidation using biotechnology to increase the stability of BAMPs and enhance their ability to pass through biological membranes [180] . Researchers have gained a profound understanding of BAMPs in recent decades. To date, they have isolated and identified hundreds of BAMPs, analyzed their functions against plant pathogens, and elucidated the mechanisms underlying the antimicrobial action of some BAMPs. However, there remains a big knowledge gap for the development of BAMPs into anti/fungicidal agents for application in plant protection. Firstly, the mechanism of action remains unclear. Some studies have reported that BAMPs interact with the cell membrane and cell wall components of pathogens, causing membrane perforation and leakage of cell contents, which achieves antimicrobial efficacy. However, the detailed process of membrane perforation caused by BAMPs requires further study. Further, some studies have reported that BAMPs can be internalized into the cytoplasm and interact with intracellular targets to mediate cellular signaling pathways, leading to apoptosis of pathogenic cells. However, it is unknown how BAMPs are internalized into cells, which targets they interact with, and how they mediate cellular signaling in pathogen cells. The lag in mechanistic studies has hindered our in-depth understanding of BAMPs. Secondly, multiple BAMPs are upregulated to participate together in the immune response when plants are infected by pathogens. In practical applications, BAMPs' synergism may be required to achieve optimal efficacy. Finally, external application of BAMPs faces limitations such as short half-lives, weaker resistance, and harsh activity conditions. Therefore, enhancing the stability of BAMPs is an inescapable problem that must be resolved prior to their industrial application. The field of peptide therapeutics is rapidly growing. To date, more than 80 peptide drugs have been approved by the FDA or the EU EMA, and dozens of AMPs are currently being evaluated in clinical trials. Although some studies report BAMP efficacy in cancer inhibition, as well as inhibition of viral replication and propagation, no drugs developed with a BAMP backbone have yet to be marketed. Lunasin is one of the more thoroughly studied BAMPs, which not only targets the chromatin in cells, but also disrupts the formation of the mitotic complex in the N-terminal region of histones and blocks normal mitosis. Additionally, lunasin is able to target tumor cell integrin receptors specifically through its RGD domain and mediate the integrin signaling pathway, thereby inhibiting tumor cell proliferation. Most importantly, lunasin is resistant to digestion and can be absorbed directly after oral administration to reach specific tissue sites, which provides a reference for the development of therapeutic foods and oral BAMP-derived drugs, and provides insight for basic research on BAMPs. Is it possible for BAMPs to interfere with signaling pathways by targeting specific tumor cell surface receptors, while being internalized intracellularly, to interact with target proteins and thus induce apoptosis or other biological process in cancer cells? Moreover, due to their short half-lives and instability, peptides are currently administered locally by intravenous injection. Developing BAMPs for direct therapeutic feeding or oral administration would be a meaningful and promising alternative. Based on synthetic biotechnology, whole edible plants or edible microalgae can be used as bioreactors, and compartmentalized synthesis and amino acid modification can be used to synthesize BAMPs, thus optimizing the structural stability of BAMPs and solving scale-up issues for the industrial production of BAMPs. Currently, the global market for peptide drugs has significant growth potential. Future in-depth research addressing the aforementioned limitations of BAMPs will enable their successful application in agriculture and human health. Due to the advantages of high yield, high quality, homogeneity, and post-translational modifications such as glycosylation and disulfide bond formation that are critical to BAMPs' activity in plant bioreactors, a plantbased BAMPs' production platform is more promising than other biological systems [176] . It is believed that plant molecular farms can play a greater role in the production of BAMPs if the yield and stability of the products can be further solved to meet the needs of the market, as well as the safety, quality, and effectiveness. Multitasking antimicrobial peptides in plant development and host defense against biotic/abiotic stress Properties and mechanisms of action of naturally occurring antifungal peptides Antimicrobial peptides of multicellular organisms Immunocontinuum: Perspectives in antimicrobial peptide mechanisms of action and resistance The antimicrobial domains of wheat puroindolines are cell-penetrating peptides with possible intracellular mechanisms of action VvAMP2, a grapevine flower-specific defensin capable of inhibiting Botrytis cinerea growth: Insights into its mode of action Membrane-interacting antifungal peptides Antimicrobial peptides modes of mechanism, modulation of defense responses PhytAMP: A database dedicated to antimicrobial plant peptides The use of versatile plant antimicrobial peptides in agribusiness and human health Antimicrobial peptides from plants Defensins-components of the innate immune system in plants SMALL cysteine-rich antifungal proteins from radish-their role in host-defense Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis Petunia floral defensins with unique prodomains as novel candidates for development of Fusarium wilt resistance in transgenic banana plants Isolation, characterization, molecular cloning and modeling of a new lipid transfer protein with antiviral and antiproliferative activities from Naycissus tazetta An approach towards structure based antimicrobial peptide design for use in development of transgenic plants: A strategy for plant disease management Significance of inducible defense-related proteins in infected plants The evolution, function and mechanisms of action for plant defensins The C-terminal propeptide of a plant defensin confers cytoprotective and subcellular targeting functions Plant defensins: Common fold, multiple functions Antibiotic activities of host defense peptides: More to it than lipid bilayer perturbation Plectasin, a fungal defensin, targets the bacterial cell wall precursor lipid II A. DmAMP1, an antifungal plant defensin from dahlia (Dahlia merckii), interacts with sphingolipids from Saccharomyces cerevisiae Interactions of antifungal plant defensins with fungal membrane components The plant defensin NaD1 introduces membrane disorder through a specific interaction with the lipid, phosphatidylinositol 4,5 bisphosphate Phosphoinositide-mediated oligomerization of a defensin induces cell lysis A gene encoding a sphingolipid biosynthesis enzyme determines the sensitivity of Saccharomyces cerevisiae to an antifungal plant defensin from dahlia (Dahlia merckii) Defensins from insects and plants interact with fungal glucosylceramides The antifungal activity of RsAFP2, a plant defensin from Raphanus sativus, involves the induction of reactive oxygen species in Candida albicans The plant defensin RsAFP2 induces cell wall stress, septin mislocalization and accumulation of ceramides in Candida albicans Antifungal plant defensins: Mechanisms of action and production Differential antifungal and calcium channel-blocking activity among structurally related plant defensins Glucosylceramide synthase is essential for alfalfa defensinmediated growth inhibition but not for pathogenicity of Fusarium graminearum Permeabilization of fungal hyphae by the plant defensin NaD1 occurs through a cell wall-dependent process The antifungal plant defensin HsAFP1 from Heuchera sanguinea induces apoptosis in Candida albicans Antifungal Pisum sativum defensin 1 interacts with Neurospora crassa cyclin F related to the cell cycle Recommendations for naming plant pathogenesis-related proteins The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins PR1-mediated defence via C-terminal peptide release is targeted by a fungal pathogen effector Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a Pathogenesis-Related PR-1 proteins are antifungal (isolation and characterization of three 14-kilodalton proteins of tomato and of a basic PR-1 of tobacco with inhibitory activity against Phytophthora infestans) The sterol-binding activity of pathogenesis-related protein 1 reveals the mode of action of an antimicrobial protein Pathogen-Related Yeast (PRY) proteins and members of the CAP superfamily are secreted sterolbinding proteins Plant and animal PR1 family members inhibit programmed cell death and suppress bacterial pathogens in plant tissues A salt-regulated peptide derived from the CAP superfamily protein negatively regulates salt-stress tolerance in Arabidopsis Quantitative peptidomics study reveals that a wound-induced peptide from PR-1 regulates immune signaling in tomato A dimeric PR-1-type pathogenesis-related protein interacts with ToxA and potentially mediates ToxA-induced necrosis in sensitive wheat PR-1 proteins are targeted by necrotrophic pathogen effector proteins A cerato-platanin protein SsCP1 targets plant PR1 and contributes to virulence of Sclerotinia sclerotiorum Plant thionins-the structural perspective Amino acid sequence of two polypeptides of a new family of thionins from wheat endosperm Processing of thionin precursors in barley leaves by a vacuolar proteinase Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum Transgenic tomato plants expressing an Arabidopsis thionin (Thi2.1) driven by fruit-inactive promoter battle against phytopathogenic attack Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens Enhanced resistance to seed-transmitted bacterial diseases in transgenic rice plants overproducing an oat cell-wall-bound thionin Interaction between beta-purothionin and dimyristoylphosphatidylglycerol: A P-31-NMR and infrared spectroscopic study Permeabilization of fungal membranes by plant defensins inhibits fungal growth Lipid transfer proteins (NSLTPS) from barley and maize leaves are potent inhibitors of bacterial and fungal plant-pathogens Cellular responses to Pyrularia thionin are mediated by Ca 2+ influx and phospholipase A2 activation and are inhibited by thionin tyrosine iodination Toxic proteins from European mistletoe (Viscum album L.: Increase of intracellular IL-4 but decrease of IFN-gamma in apoptotic cells Structure of beta-purothionin in membranes: A two-dimensional infrared correlation spectroscopy study Lipid transfer proteins: Classification, nomenclature, structure, and function Plant defense and antimicrobial peptides Structural stability and surface activity of sunflower 2s albumins and nonspecific lipid transfer protein Plant lipid transfer proteins: Are we finally closing in on the roles of these enigmatic proteins? Role of plant lipid transfer proteins in plant cell physiology-A concise review Lipid transfer proteins enhance cell wall extension in tobacco Nonspecific lipid transfer protein in castor bean cotyledon cells subcellular localization and a possible role in lipid-metabolism The Arabidopsis lipid transfer protein 2 (AtLTP2) is involved in cuticle-cell wall interface integrity and in etiolated hypocotyl permeability The nonspecific lipid transfer protein AtLtpI-4 is involved in suberin formation of Arabidopsis thaliana crown galls Arabidopsis LTPG Is a glycosylphosphatidylinositolanchored lipid transfer protein required for export of lipids to the plant surface Cuticular defects lead to full immunity to a major plant pathogen Enhanced tolerance to bacterial pathogens caused by the transgenic expression of barley lipid transfer protein LTP2 Characterisation, immunolocalisation and antifungal activity of a lipid transfer protein from chili pepper (Capsicum annuum) seeds with novel alpha-amylase inhibitory properties Stress induction and antimicrobial properties of a lipid transfer protein in germinating sunflower seeds A potent antimicrobial protein from onion seeds showing sequence homology to plant lipid transfer proteins In vitro antifungal activity of a radish (Raphanus sativus L.) seed protein homologous to nonspecific lipid transfer proteins The cytotoxic properties of a plant lipid transfer protein involve membrane permeabilization of target cells Lipid-transfer proteins in plants Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens Plant pathogenesis-related (PR) proteins: A focus on PR peptides Plant serine proteinase inhibitors Protein proteinase inhibitor genes in combat against insects, pests, and pathogens: Natural and engineered phytoprotection Role of inhibitors of proteolytic enzymes in plant defense against phytopathogenic microorganisms Proteinase inhibitors as antistress proteins in higher plants The use of cysteine proteinase inhibitors to engineer resistance against potyviruses in transgenic tobacco plants Expression of the maize proteinase inhibitor (mpi) gene in rice plants enhances resistance against the striped stem borer (Chilo suppressalis): Effects on larval growth and insect gut proteinases Increased proteinase-inhibitor activity in response to infection of resistant tomato plants by Phytophthora infestans Differential expression of tomato proteinase inhibitor-i and inhibitor-ii genes during bacterial pathogen invasion and wounding Synergistic enhancement of the antifungal activity of wheat and barley thionins by radish and oilseed rape-2S albumins and by barley trypsin-inhibitors Characterization of peptides from Capsicum annuum hybrid seeds with inhibitory activity against α-amylase, serine proteinases and fungi An aspartic proteinase gene family in the filamentous fungus Botrytis cinerea contains members with novel features Synergistic interaction between fungal cell-wall degrading enzymes and different antifungal compounds enhances inhibition of spore germination Purification and characterization of membrane-bound chitin synthase Two mitogen-activated protein kinase signalling cascades mediate basal resistance to antifungal plant defensins in Fusarium graminearum Refinement of purothionins reveals solute particles important for lattice formation and toxicity. Part 1: Alpha1-purothionin revisited Mistletoe viscotoxins induce membrane permeabilization and spore death in phytopathogenic fungi Emerging fungal threats to animal, plant and ecosystem health The proteome targets of intracellular targeting antimicrobial peptides X-ray structure of a carpet-like antimicrobial defensin-phospholipid membrane disruption complex Presence of glucosylceramide in yeast and its relation to alkali tolerance of yeast In vitro activity of the antifungal plant defensin RsAFP2 against Candida isolates and its in vivo efficacy in prophylactic murine models of candidiasis Systematic screens of a Candida albicans homozygous deletion library decouple morphogenetic switching and pathogenicity The fungal cell wall: Candida, Cryptococcus, and Aspergillus species Human antibodies against a purified glucosylceramide from Cryptococcus neoformans inhibit cell budding and fungal growth Pn-AMP1, a plant defense protein, induces actin depolarization in yeasts Human antimicrobial peptide LL-37 inhibits adhesion of Candida albicans by interacting with yeast cell-wall carbohydrates Antifungal potency and modes of action of a novel olive tree defensin against closely related ascomycete fungal pathogens StPIP1, a PAMPinduced peptide in potato, elicits plant defenses and is associated with disease symptom severity in a compatible interaction with Potato virus Y Peptide aptamers that bind to geminivirus replication proteins confer a resistance phenotype to tomato yellow leaf curl virus and tomato mottle virus infection in tomato A peptide derived from enzymatic digestion of globulins from amaranth shows strong affinity binding to the replication origin of Tomato yellow leaf curl virus reducing viral replication in Nicotiana benthamiana Peptide-mediated broad-spectrum plant resistance to tospoviruses Ginkbilobin, a novel antifungal protein from Ginkgo biloba seeds with sequence similarity to embryoabundant protein Ascalin, a new anti-fungal peptide with human immunodeficiency virus type 1 reverse transcriptaseinhibiting activity from shallot bulbs Lunatusin, a trypsin-stable antimicrobial peptide from lima beans (Phaseolus lunatus L.). Peptides Vulgarinin, a broad-spectrum antifungal peptide from haricot beans (Phaseolus vulgaris) Inhibition of foot and mouth disease virus (FMDV) uncoating by a plant-derived peptide isolated from Melia azedarach L leaves Novel bioactive peptides demonstrating anti-dengue virus activity isolated from the Asian medicinal plant Acacia Catechu A synthetic bioactive peptide derived from the asian medicinal plant Acacia Catechu binds to dengue virus and inhibits cell entry Lectin-mediated resistance impairs plant virus infection at the cellular level The Plant noncanonical antiviral resistance protein JAX1 inhibits potexviral replication by targeting the viral RNA-dependent RNA polymerase Beclin1 restricts RNA virus infection in plants through suppression and degradation of the viral polymerase The TPR domain in the host Cyp40-like cyclophilin binds to the viral replication protein and inhibits the assembly of the tombusviral replicase Antimicrobial host defence peptides: Functions and clinical potential Role of human beta-defensin-2 during tumor necrosis factor-alpha/NF-kappa B-mediated innate antiviral response against human respiratory syncytial virus Anti-HIV-1 activity of indolicidin, an antimicrobial peptide from neutrophils Mechanism of adenovirus neutralization by human alpha-defensins Multivalent binding of carbohydrates by the human alphadefensin, HD5 Activity of alpha-and thetadefensins against primary isolates of HIV-1 Human cytomegalovirus binding to heparan sulfate proteoglycans on the cell surface and/or entry stimulates the expression of human leukocyte antigen class I Theta defensins protect cells from infection by herpes simplex virus by inhibiting viral adhesion and entry Anti-HSV activity of lactoferrin and lactoferricin is dependent on the presence of heparan sulphate at the cell surface Cathelicidins display conserved direct antiviral activity towards rhinovirus Lunasin: A cancer-preventive soy peptide Barley lunasin suppresses ras-induced colony formation and inhibits core histone acetylation in mammalian cells The cancer preventive peptide lunasin from wheat inhibits core histone acetylation Anticancer and Chemosensitizing Abilities of cycloviolacin O2 from Viola odorata and psyle cyclotides from Psychotria leptothyrsa In vivo activation of the p53 tumor suppressor pathway by an engineered cyclotide Novel cyclotides from Hedyotis biflora inhibit proliferation and migration of pancreatic cancer cell in vitro and in vivo Nicotiana alata defensin chimeras reveal differences in the mechanism of fungal and tumor cell killing and an enhanced antifungal variant Determination of primary structure of a novel peptide from mistletoe and its antitumor activity Ligatoxin B, a new cytotoxic protein with a novel helix-turn-helix DNA-binding domain from the mistletoe Phoradendron liga Membrane lipids: Where they are and how they behave Small, novel proteins from the mistletoe Phoradendron tomentosum exhibit highly, selective cytotoxicity to human breast cancer cells Thionin Thi2.1 from Arabidopsis thaliana expressed in endothelial cells shows antibacterial, antifungal and cytotoxic activity Sesquin, a potent defensin-like antimicrobial peptide from ground beans with inhibitory activities toward tumor cells and HIV-1 reverse transcriptase Limenin, a defensin-like peptide with multiple exploitable activities from shelf beans Coccinin, an antifungal peptide with antiproliferative and HIV-1 reverse transcriptase inhibitory activities from large scarlet runner beans Cn-AMP1: A new promiscuous peptide with potential for microbial infections treatment Identification and characterization of a bactericidal and proapoptotic peptide from cycas revoluta seeds with DNA binding properties A cytotoxic cyclic heptapeptide from the seeds of Annona cherimola Identification and structural characterization of a new pro-apoptotic cyclic octapeptide cyclosaplin from somatic seedlings of Santalum album L Inhibition of breast cancer cell migration by cyclotides isolated from Pombalia calceolaria IbACP, a sixteen-amino-acid peptide isolated from Ipomoea batatas leaves, induces carcinoma cell apoptosis From antimicrobial to anticancer peptides. A review Cationic amphiphilic peptides with cancer-selective toxicity Anticancer potential of bioactive peptides from animal sources (Review) Cellular integrins function as entry receptors for human cytomegalovirus via a highly conserved disintegrin-like domain A defensin with highly potent antipathogenic activities from the seeds of purple pole bean Dietary peptides from the non-digestible fraction of Phaseolus vulgaris L. decrease angiotensin II-dependent proliferation in HCT116 human colorectal cancer cells through the blockade of the renin-angiotensin system Antimicrobial peptides as effective tools for enhanced disease resistance in plants Plant defensins: Types, mechanism of action and prospects of genetic engineering for enhanced disease resistance in plants Pn-AMPs, the hevein-like proteins from Pharbitis nil confers disease resistance against phytopathogenic fungi in tomato, Lycopersicum esculentum Identification of pathogen-responsive regions in the promoter of a pepper lipid transfer protein gene (CALTPI) and the enhanced resistance of the CALTPI transgenic Arabidopsis against pathogen and environmental stresses Transgene stacking and coordinated expression of plant defensins confer fungal resistance in rice Enhanced resistance to the rice blast fungus Magnaporthe grisea conferred by expression of a cecropin A gene in transgenic rice Production of cecropin A antimicrobial peptide in rice seed endosperm Expression of a synthesized gene encoding cationic peptide cecropin B in transgenic tomato plants protects against bacterial diseases The production of recombinant cationic α-helical antimicrobial peptides in plant cells induces the formation of protein bodies derived from the endoplasmic reticulum Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: Impact on rice host plant fitness Antimicrobial peptides: A promising therapeutic strategy in tackling antimicrobial resistance Antimicrobial peptides under clinical trials Antimicrobial peptide production and plant-based expression systems for medical and agricultural biotechnology Expression and characterization of antimicrobial peptides Retrocyclin-101 and Protegrin-1 in chloroplasts to control viral and bacterial infections High-level production of lactostatin, a hypocholesterolemic peptide, in transgenic rice using soybean A1aB1b as carrier From structure to function Synthetic therapeutic peptides: Science and market We acknowledge all the members of the functional plant innovation team for their valuable comments on the manuscript. The authors declare no competing interests.