key: cord-0865254-qmgj6tmk authors: Spoto, Silvia; Valeriani, Emanuele; Riva, Elisabetta; De Cesaris, Marina; Tonini, Giuseppe; Vincenzi, Bruno; Locorriere, Luciana; Beretta Anguissola, Giuseppina; Lauria Pantano, Angelo; Brando, Elisa; Costantino, Sebastiano; Ciccozzi, Massimo; Angeletti, Silvia title: A Staphylococcus aureus Coinfection on a COVID-19 Pneumonia in a Breast Cancer Patient date: 2020-09-30 journal: Int J Gen Med DOI: 10.2147/ijgm.s261760 sha: d46c2728c6e4cef00c70f9d35d25493dca82d3a2 doc_id: 865254 cord_uid: qmgj6tmk INTRODUCTION: Coronavirus disease 19 (COVID-19), due to severe acute respiratory syndrome-coronavirus 2 (SARS-CoV2), comprises a broad spectrum of clinical presentation ranging from flu-like syndrome to organ failure. The risk of coinfections is high and responsible for a worse prognosis, mainly in the case of bacterial involvement and in the presence of particular comorbidity. We present the clinical, laboratory, radiologic characteristic along with therapeutic management of a patient with COVID-19 and Staphylococcus aureus coinfection. CASE PRESENTATION: A 55-year-old Caucasian woman was admitted to our hospital due to a two-day history of fever and acute dyspnea with severe respiratory failure worsened after the administration of atezolizumab and nab-paclitaxel. Her medical history comprehended a triple negative, BRCA1-related, PD-L1 positive right breast cancer with multiple bone metastasis, causing bone marrow infiltration-related severe pancytopenia. Her physical examination revealed scattered wheezes, rales, and bilateral dry crackles in the middle and lower lung fields and lower limb paresis. The body mass index was 30 kg/m(2) and arterial blood gas evaluation revealed a stage III acute respiratory distress syndrome. Microbiological specimens revealed a Staphylococcus aureus positivity from endotracheal aspirate. The chest computed tomography (CT) scan showed the presence of large areas of parenchymal consolidation and aerial bronchogram, bilateral “ground glass” areas reaching the highest extension on the upper and middle zones. The high clinical and radiological suspicion of COVID-19 along with the negative result of nasopharyngeal specimen make necessary an endotracheal aspirate resulting positive for SARS-CoV2. Patient started an antimicrobial treatment and lopinavir-ritonavir plus hydroxychloroquine but, unfortunately, died five days after hospital admission. CONCLUSION: The high risk of mortality of our patient was due to viral-bacterial coinfection, advanced cancer status with active immunotherapy. This case highlights the need for a prompt clinical, laboratory, and radiological evaluation to allow a correct diagnosis and start a specific therapy. Severe acute respiratory syndrome-Coronavirus 2 (SARS-CoV2) is a new onset virus belonging to Coronaviridae family causing flu-like syndrome to organ failure with high risk of coinfections. 1 A wide range of computed tomography (CT) findings could be associated with COVID-19 pneumonia including both the interstitial involvement and pulmonary consolidation, respectively associated with a viral or bacterial infection. The early stage of the COVID-19 pneumonia, indeed, is characterized by ground-glass opacities that progress to a crazy-paving pattern and multifocal patchy consolidation. [2] [3] [4] [5] Currently, viral and bacterial pathogens are responsible for 38% and 11% of adult community-acquired pneumonia, respectively. 6 The 30-day mortality of a viral pneumonia is 7.1% and is associated with older age, viral-bacterial coinfection, underlying malignancy, and shock. 7 Coinfection by COVID- 19 and Influenza virus did not significantly worsen the outcome; 8 otherwise, viral-bacterial coinfection showed higher mortality rates than unique viral infection up to 60%. 7, 9 Viral infection could facilitate bacterial superinfection by virus-induced airway damage, promoting bacterial adherence, decreasing mucociliary clearance and impairing the immune system. 10 Coronavirus-related pneumonia (no COVID-19) showed higher mortality in cancer than in noncancer patients (24.3% vs 3%), 7 and in immunocompromised patients. 11 The prognosis of influenza virus infection in cancer patients was affected by many other factors such as bacterial or fungal superinfection, time to diagnosis, and immunosuppression therapy. 12 A 55-year-old Caucasian woman was admitted to our Hospital after a two-day history of fever (38.5°C) and acute dyspnea with severe respiratory distress and roomair oxygen saturation of 70% occurring few hours after the administration of chemoimmunotherapy. The patient was diagnosed with a triple negative, BRCA1-related, PD-L1 positive right breast cancer four years earlier and was treated with quadrantectomy, radiotherapy and adriamycin-cyclophosphamide and docetaxelbased adjuvant chemotherapy. The tumor had spread with multiple bone metastasis -skull, vertebral, sternum, scapula, ribs, and femurs-and caused bone marrow infiltration-related severe pancytopenia. She was treated with atezolizumab and nab-paclitaxel as first line chemotherapy. She suspended, two weeks before, the self-administration of Ganoderma lucidum medicinal mushrooms as an anticancer supplement. Patient recently received flu vaccination and her remaining medical history comprised chemical diabetes. The physical examination revealed Glascow Coma Scale 14, body temperature 37.7°C, blood pressure 105/ 60 mmHg, heart rate 117 beats/minute, respiratory rate 25 breaths/minute, oxygen saturation 90% while receiving a 100% fraction of inspired oxygen (FiO 2 ) on reservoir mask -pH 7.55; pCO 2 30 mmHg; pO 2 87 mmHg, arterial bicarbonate level of 27.5 mmol/L, arterial lactated level of 21, partial pressure of oxygen (pO 2 )/FiO 2 87 mmHg (stage III Acute Respiratory Distress Syndrome)-BMI 30 kg/m 2 . Chest examination showed scattered wheezes, rales, and bilateral dry crackles in the middle and lower lung fields; regular cardiac rhythm with paraphonic cardiac sounds. Neurological examination revealed lower limb paresis. Laboratory tests results included hemoglobin of 6.7 g/ dL, hematocrit of 20.2%, platelet count of 18,000/µL, white blood cells of 2210/µL -neutrophil and lymphocytic count of 1720 (77%) and 440/µL (19.9%), respectively-erythrocyte sedimentation rate of 76 mm/h, C-reactive protein of 43.06 mg/dL, procalcitonin of 0.55 ng/dL, and albumin of 2.6 g/dL. The microbiological sample results for Chlamydia pneumoniae, Mycoplasma pneumoniae, Respiratory Syncytial, Adenoviruses, Coxsackieviruses, Influenza A and B viruses turned out negative, as well as the nasopharyngeal swab for SARS-CoV2. Further tests included negative Streptococcus pneumoniae and Legionella pneumophila urinary antigens, serum galactomannan assay, beta D-glucan assay, immunofluorescence assay for the detection of Pneumocystis jirovecii and blood culture. Quantiferon TB Gold test resulted indeterminate, and Staphylococcus aureus was isolated from endotracheal aspirate. The chest computed tomography (CT) scan showed the presence of large areas of parenchymal consolidation with aerial bronchogram, bilateral "ground glass" areas reaching the highest extension on the upper and middle zones, and a moderate bilateral pleural effusion. Nonspecific subcentimetric ilo-mediastinal lymph nodes and several metastatic lytic bone lesions were also revealed ( Figure 1A -C). The artificial intelligence software (CT deep learning algorithm) estimated a positive predictive value of 64% for COVID-1-related pneumonia. The chest physical examination was suggestive for interstitial pneumonia that was confirmed by the CT scan. Furthermore, based on current epidemiology, the oncologic patient's frailty suggested the need for an endotracheal aspirate detecting spike protein (S) and envelope (E) genes for SARS-CoV2 molecular test that resulted positive (Figure 2A) . The bilateral lung consolidation along with the interstitial radiologic pattern and the recent history of G. lucidum administration questioned the real nature of the pneumonia. However, to date, no data are available on fungal pneumonia due to G. lucidum administration. The patient started an empiric antimicrobial treatment with vancomycin 2 g/day, meropenem 3 g/day, and levofloxacin 500 mg/day along with amphotericin B therapy (loading dose of 6 mg/kg bid, followed by 300 mg bid) and high flow oxygen supplementation (FiO 2 100%). Otherwise, the endotracheal aspirates showed positive results for SARS-CoV2 and for methicillin-sensible S. aureus. 13 Antimicrobial therapy was, therefore, de-escalated and lopinavir-ritonavir plus hydroxychloroquine therapy was started. Unfortunately, the patient died five days after hospital admission. Viral infection contributes in bacterial spread providing more adhesion sites, causing cell and tissue structural alteration, and impairing immune system response. Bacterial coinfection was responsible for a worse prognosis characterized by higher disease severity also influenced by viral and bacterial strains, density of bacterial colonization, and the time between viral infection and bacterial coinfection. 14 S. aureus, a Gram-positive coccus, may complicate Influenza infection that increase the S. aureus adherence to the host pharyngeal cells. This phenomenon increases patients' mortality within two to seven days from bacterial coinfection. 15 Available evidence does not allow an accurate estimation of the bacterial coinfection prevalence on COVID- 19. In this case, the bacterial coinfection has hidden the typical radiologic feature of COVID-19-related pneumonia. 2, 3 However, the clinical findings along with the high prevalence of the disease suggested the execution of an endotracheal rather than nasopharyngeal aspirate for SARS-CoV2 that showed a sensitivity of 95% when correctly collected. Interestingly, we performed a qSARS-CoV-2 IgG/IgM cassette rapid test two days after COVID-19 diagnosis showing negative results ( Figure 2B ). This could be the consequence of a failure of the immunological memory, the patient's immunosuppression state, or the lower test sensitivity (87.9%). Respiratory viruses are pathogens of 38% of communityacquired pneumonia, but they can also be responsible for coinfections or bacterial lung superinfections. 6 The influenza virus is known to increase the susceptibility to pneumonia caused by S. aureus. Furthermore, this latter caused 4% of sepsis among hospitalized patients with cancer. 16 Bacterial superinfection should never be excluded and, in this case, changed the classical radiological alteration lowering the positive predictive value of the artificial intelligence software. Otherwise, immunotherapy could mimic the infectious tomographic pattern, as previously described. 2, 3 Being the epidemic in Lazio Region represented mostly in big family and rest home clusters, it is important to monitor specific cases, when possible. Clinical diagnosis is a primary and important step sustained by CT and laboratory confirmation. The reported mortality ranged from 4.3% to 15% in China. The high mortality rate reported in Italy (actually, 11.4%) may be related to an underestimation of the prevalence of the disease related to the quote of asymptomatic population. [17] [18] [19] [20] We present a case of a breast cancer patient receiving immunotherapy, in which the bacterial coinfection could have covered the radiological diagnosis of Coronavirus pneumoniae, allowed only by the clinical-laboratory and radiological integration. The high risk of death of our patient related to viralbacterial coinfection was also increased by the advanced cancer status with active immunotherapy. Moreover, these latter agents may be involved in a more aggressive clinical behavior of viral infections, even if data are still premature and under investigation. COVID-19 is a systemic disease that can affect several organs or systems (respiratory, cardiac, nervous, gastrointestinal systems, kidney, and skin) with high risk of superinfections and pulmonary embolism, requiring several clinical skills along with a specific radiological and laboratory workup. COVID-19, coronavirus disease 19; SARS-CoV2, severe acute respiratory syndrome-coronavirus 2; CT, computed tomography; BMI, body mass index. The data that support the findings of this study are available on request from the corresponding author, SS. Based on the regulations of the department of research of the University Campus Bio-Medico of Rome, an institutional review board approval is not required for case reports. The patient provided informed consent for publication of the case details and images. The International Journal of General Medicine is an international, peer-reviewed open-access journal that focuses on general and internal medicine, pathogenesis, epidemiology, diagnosis, monitoring and treatment protocols. The journal is characterized by the rapid reporting of reviews, original research and clinical studies across all disease areas. The manuscript management system is completely online and includes a very quick and fair peer-review system, which is all easy to use. 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The authors declared that this case has received no financial support. The authors report no conflicts of interest in this work.