key: cord-0867021-obdguvo8 authors: Fu, Zhao; Tang, Ningning; Chen, Yanqing; Ma, Longbai; Wei, Youyong; Lu, Yumin; Ye, Kun; Liu, Hang; Tang, Fen; Huang, Guangyi; Yang, Yingxia; Xu, Fan title: CT features of COVID-19 patients with two consecutive negative RT-PCR tests after treatment date: 2020-07-14 journal: Sci Rep DOI: 10.1038/s41598-020-68509-x sha: f57ec60a031b07fe03a369446a14298ae2653ef7 doc_id: 867021 cord_uid: obdguvo8 The objective of this study is to expound the CT features of COVID-19 patients whose throat swab samples were negative for two consecutive nucleic acid tests after treatment. We retrospectively reviewed 46 COVID-19 patients with two consecutive negative RT-PCR tests after treatment. The cases were divided into moderate group and severe/critical group according to disease severity. Clinical and CT scanning data were collected. CT signs of pulmonary lesions and the score of lung involvement were expounded. Thirty-nine moderate cases and seven severe/critical cases were included. Residual pulmonary lesions were visible in CT images. Moderate patients showed peripheral lesions while severe/critical cases exhibited both central and peripheral lesions with all lobes involvement. Mixed ground glass opacity (GGO) and pulmonary consolidation were noted. A larger proportion of severe patients showed reticular pulmonary interstitium thickening. Air bronchogram, pleural effusion, vascular enlargement, bronchial wall thickening, bronchiectasis, pleural thickening and pleural adhesion were more frequently observed in severe/critical group. The severe/critical group showed higher CT score. Pulmonary lesions persisted even after twice consecutive negative nucleic acid tests. We strongly recommended regular follow-up of CT scans after nucleic acid tests conversion. Evaluation of complete remission should base on chest CT. Clinical characteristics. A total of 46 patients were included in this study, including 39 cases with moderate COVID-19 and 7 cases with severe/critical COVID-19 (shown in Table 1 ). The average age was greater in the severe/critical group than in the moderate group (46.2 ± 13.5 vs 57.9 ± 17.0, P = 0.049). There was no statistical difference in gender between the two groups (P = 0.68). There was no statistically significant difference in symptoms between the two groups. Only 4 (10%) moderate patients and 1 (14%) severe patient presented with fever on admission. Other clinical symptoms of COVID-19 included dry cough, cough with or without sputum, chest tightness, polypnea, fatigue, diarrhea and throat discomfort. CT findings. Residual pulmonary lesions were visible despite two consecutive negative RT-PCR tests (shown in Table 2 and Fig. 1 ). Multiple lesions were showed in both moderate and severe/critical group (92% vs 100%, P = 1.0). There was a statistically significant difference in lesion distribution between the two groups (P = 0.005). Peripheral lesions were predominant in moderate group (85%) while lesions in both peripheral and central regions were common in severe/critical group (71%). Extensive lesions with five lobes involvement were more significant in severe/critical group than in the moderate group (100% vs 44%, P = 0.01). Mixed ground glass opacity and pulmonary consolidation were more frequently observed in severe/critical group than moderate group (100% vs 41%, P = 0.009; 57% vs 10%, P = 0.012, respectively). Comparison of lesion shape revealed no statistical difference except fan-shaped lesions, which is more common in severe/critical group than moderate group (100% vs 49%, P = 0.014). A larger proportion of patients showed reticular pulmonary interstitium thickening in severe/critical group than moderate group (86% vs 59%, P = 0.043). Air bronchogram and pleural effusion were significant more frequent within severe/critical group compared to moderate group (57% vs 3%, P = 0.003; 43% vs 3%, P = 0.009, respectively). In addition, vascular enlargement, bronchial wall thickening, bronchiectasis, pleural thickening and pleural adhesion were more frequently observed in severe/critical group, although the differences were not statistically significant (P = 0.316, 0.160, 0.089, 0.106, 0.083, respectively). Total CT score were significantly higher in severe/critical group compared to moderate group. Most patients in moderate group ranged from 0 to 5 (74%), whereas a majority of patients in severe/critical group ranged between 16 and 20 (57%), which was consistent with the extensive involvement of lesions in severe/critical group. Achieving two consecutive negative results of nucleic acid tests has currently been recognized as the most important treatment end point for COVID-19 patients. However, our study demonstrated that the pulmonary lesions persisted even after RT-PCR conversion. Multiple lesions such as GGO, pulmonary interstitium thickening and pleural effusion remained common when nucleic acid tests were negative, indicating the presence of dyssynchrony between SARS-CoV-2 nucleic acid tests and chest CT abnormalities. Multiple lesions with multiple lung lobes involvement were noted in the CT images. The moderate group typically presented with lung peripheral lesions, while the severe group exhibited both peripheral and central lesions. This was similar with other earlier COVID-19 reports [5] [6] [7] [9] [10] [11] . The main pattern of lesions was irregular in this study, which was different from the circular and fan-shaped lesions in early stage of the disease 5, 12 . It is probably related to the natural progression of COVID-19. Irregular signs might result from unsynchronized lesion absorption and inter-fusion. In addition, pulmonary fibrosis was observed in five severe patients, which was the result of lesion absorption and recovery. GGO remained the most common finding after nucleic acid test conversion. In contrast to the early stage of the disease, mixed GGO and consolidation was dominant after treatment 9 . What we have to point out is that air bronchogram could be found in consolidation lesion and some patients had visible bronchial wall thickening and bronchiectasis, which showed inflammation in the bronchi of the lungs. Chest tightness 2 (5%) 0 (0%) - www.nature.com/scientificreports/ Pulmonary interstitium thickening is another important sign of COVID-19 pneumonia, which showed more apparent in CT images after nucleic acid tests conversion. Linear pulmonary interstitium thickening was dominated in the early stage, while reticular thickening was dominated after treatment. Pleural thickening and pleural adhesion in COVID-19 patients were rarely reported to date 6, 10 . However, visible pleural thickening was observed in half of the moderate patients, of whom a majority presented with pleural adhesion simultaneously. Pleural thickening and pleural adhesion were even more common in severe cases. In addition, small amount of bilateral pleural effusion was observed in one moderate patient and three severe patients. The pleural abnormalities indicated pleural inflammation in COVID-19 patients, especially in severe cases. The lung scoring method was used to reflect the approximate range of COVID-19 pneumonia. The score of moderate patients were mostly (29 of 39 patients) between 0 and 5, while the score of severe patients were mostly (four of five patients) between 16 and 20. The result showed that the range of residual pulmonary lesion was wider in severe/critical patients than in moderate patients despite two consecutive negative RT-PCR tests. In clinical practice, two consecutive negative nucleic acid tests were regarded as the most important basis for discharge, however, it should be interpreted with caution, since, in our study, two consecutive negative RT-PCR tests did not signify complete cure of COVID-19 pneumonia. Even though antiviral treatment resulted in progressively lower levels of SARS-CoV-2 until the virus is no longer detectable, the tissue damage caused by overexuberant inflammatory response 13 was far from complete restoring, instead, aggravation coexists with recovery, as observed in the CT images. Although we cannot exclude the possibility that laboratorial error could have contributed to some of the inconsistency between nucleic acid tests and chest CT manifestations, the patterns of CT lesions observed in www.nature.com/scientificreports/ this study suggest that the bulk of the discrepant results reflected the persistence of pulmonary damage despite negative nucleic acid tests. Based upon these results, we would specifically discourage the use of nucleic acid tests results alone for treatment discontinuation and quarantine release decisions, while regular chest CT scans were strongly recommended even after nucleic acid tests conversion to monitor post-treatment cure. There are some limitations in this study. Firstly, the time from negative nucleic test to CT scanning was not exactly the same, as CT reexaminations were carried out at different time of intervals according to each patient's condition, which was longer for moderated cases and shorter for severe cases. Secondly, we had not performed further investigation of the pulmonary lesions, due to the lack of inspection equipment in temporary isolation wards. Bronchoscopy, bronchoalveolar lavage and lung biopsy are required to further confirm the nature of the lesions. Thirdly, although a specialized feedback and information sharing system was established between our hospital and other local hospitals to monitor the status of patients after discharge, no re-positive results of nucleic acid tests have been reported up to now. Therefore, this study failed to compare the CT findings of patients with re-positive RT-PCR tests and those with persistent negative RT-PCR tests. We will continue to pay close attention to it. In conclusion, residual pulmonary lesions remained significant after nucleic acid tests were negative, and became more sophisticated and diverse in comparison with that in earlier stage. These findings provided important insights for pathological mechanism and therapeutic efficacy evaluation of COVID-19, suggesting that chest CT was better than nucleic acid conversion in assessing the final treatment outcomes of the patients. Our results highlighted the importance of using both chest CT and nucleic acid test rather than nucleic acid test alone for monitoring of COVID-19 patients. Evaluation of complete remission should base on chest CT. Study population. Forty-six consecutive patients were included in this retrospective study. The inclusion criteria were as follows: (1) COVID-19 patients who were treated in the People's Hospital of Guangxi Zhuang Autonomous Region from February 16, 2020 to March 8, 2020; (2) the throat swab samples were negative for two consecutive nucleic acid tests (obtained at least 24 h apart) after treatment; (3) chest CT was performed after the two negative nucleic acid tests. Patients without CT findings were excluded. The patients were grouped based on the illness severity defined by the National Health Commission of China 14 . 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Diagnosis and treatment protocols of pneumonia caused by a novel coronavirus The authors wish to thank the medical, nursing and support staff at People's Hospital of Guangxi Zhuang Autonomous Region, for their help and support during the outbreak of COVID-19 and during the preparation of this review. This research was supported by Guangxi Critical Infectious Disease Center (2020281). The authors declare no competing interests.