key: cord-0873668-pcknl33c
authors: DA FONSECA, ISABEL PEREIRA; FAZENDEIRO, ISABEL; ANTUNES, FRANCISCO
title: Genetic Characterization of Cryptosporidium parvum Isolates from Cattle in Portugal: Animal and Human Implications
date: 2005-07-11
journal: J Eukaryot Microbiol
DOI: 10.1111/j.1550-7408.2001.tb00443.x
sha: 6a282e06ff1945059838f8cc5e181aa0a5cd726f
doc_id: 873668
cord_uid: pcknl33c

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transmission does occur and several genotypes of C. parvwn are well phenylindole (DAPQ was used to evaluate oocysts viability. known. PCR-RFLP is one of the techniques applied to study genetic polymorphisms.

In Portugal, the prevalence of bovine CVPtmwdiosis ranges between 12% in the North and 44% in the and 34-04 (33/97) in "Ribatejo e Oeste" regions using the W-ELISA technique. GOUP 3 with 47.2% (17/36) and group 2 with 41.7% (53/127) were the age groups which showed higher prevalences of cryptosporidiosis. Infections of 9.5% (2/21) and 10.8% (4/37) were detected in calves (1-7days old) and adults, respectively. These animals were assymptomatic carriers.

results of COWp and TRAp-Cl genes by PCR in the two studied regions showed that 58.6% (14/24) of the isolates were positive for TRAPc1 from which 71.4% were from "Ribatejo e Oeste" and 40.0% from "Alentejo". For COWP, the genomic DNA was amplified in 25.0% (6/24) of the isolates; 14.3% were from "Ribatejo e Oeste" and 40.0% from "Alentejo". TRAP-C1+ COWP were detected, in 16.7% (4/24) of the samples; 14.3% were from "Ribatejo e Oeste" and 20.0% were from "Alentejo". The sample sizes were too small to the cattle genotype was me MATERIALS AND METHODS Epidemiolo&al study -A total of 553 faecal samples from cattle were collected between 1996 and February 1998. At allow meaningful statistical analysis on geographical differences. "Alentejo", 456 samples were obtained and at "Ribatejo e Oeste" the number of samples was 97. Twelve age groups were defined: Group 1 (1-7 days old), group 2 (8-15), group 3 (16-24). group 4 (25-32), group After ER-RFLP of the 24 isolates, found ( Genotyping of C. parvurn isolates -Genomic DNA was extracted from 186 samples (128 positives and 58 negatives) and purified by two methds: guanidinium thiocyanate/silica and proteinase w e a t followed by ethanol precipitation. Two pairs of primers were used in the PCR [3, 4] . In conclusion, the prevalence of bovine cryptosporidiosis was high at tbc two studied regions. The only genotype found was the bovine type, which is common to animals and man, suggesting that C. parvum transmission is not necessarily a zoonosis. although zoonotic &ansmission can also occurs. Bovine play an important role as nservoirS for human cryptosporidiosis and also as a source of mvirmental contamination. Although viability studies with DAPI were not conclusive. oocysts were observed in water used for calves. 'his fact can contribute for the spread of animal cryptosporidiosis, environmental contamination and public health risks. Due to the small number of oocysts found in the water samples, determination of the irolates'genotype was not possible. Cryptosporidiosis study at Portugal needs to be pursuit by genotyping more isolates from faecal, animal drinking water and farm effluent samples using other molecular markers. 

Detection of Cryptosporidium oocysts in waters from animal farms. Lucrari dinttrue -Medicina Veterinaria

Importincia epidemiol6gica da detemh@o da origem dos isolados de Cryptosporidium parvum por PCR-RFLP (reaqiio em cadeia da polimerase -polimorfismo do cornprimento dos fragmentos de d @ o )

Cryptosporidium parvum: PCR-RFLP analysis of TRAP-Cl (thrombospondin-related adhesive protein of Cryptosporidium-1) gene discriminates between two alleles differentially associated with parasite isolates of animal and human origin

-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin

Natural history and biology of Cryprosporidium parvwn

Method 1622: Cryptosporidium in Water by Filtration/IMS/FA. Publication n* EPA 821-R-97-023