key: cord-0875042-mquzxdks authors: Lange, Clemens; Wolf, Julian; Auw-Haedrich, Claudia; Schlecht, Anja; Boneva, Stefaniya; Lapp, Thabo; Agostini, Hansjürgen; Martin, Gottfried; Reinhard, Thomas; Schlunck, Günther title: What is the significance of the conjunctiva as a potential transmission route for SARS-CoV-2 infections? date: 2020-11-03 journal: Ophthalmologe DOI: 10.1007/s00347-020-01255-7 sha: 4162541309b7a066baa60cbb6251124a63867bec doc_id: 875042 cord_uid: mquzxdks Recent studies have described conjunctivitis in approximately 1% of COVID-19 patients and speculated that SARS-CoV‑2 can be transmitted via the conjunctiva. In this article we recapitulate the molecular mechanisms of host cell entry of SARS-CoV‑2 and discuss the current evidence for a potential conjunctival transmission of SARS-CoV‑2. The current body of evidence indicates that SARS-CoV‑2 requires the membrane-bound angiotensin-converting enzyme 2 (ACE2) and the membrane-bound serine protease TMPRSS2 to enter cells. Recent studies suggest that COVID-19 patients rarely exhibit viral RNA in tear film and conjunctival smears and that, ACE2 and TMPRSS2 are only expressed in small amounts in the conjunctiva, making conjunctival infection with SARS-CoV‑2 via these mediators unlikely. Nevertheless, we consider the current evidence to be still too limited to provide a conclusive statement and recommend appropriate protective measures for healthcare personnel who are in close contact with suspected and confirmed COVID-19 patients. The WHO declared the outbreak of COVID-19 a health emergency of international concern. COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and is associated with symptoms such as fever, cough, impaired taste, fatigue, and severe pneumonia [19] . SARS-CoV-2 is highly infectious and primarily transmitted via inhalation of aerosol droplets released by an infected individual, as well as possibly via the feco-oral route [6] . Potential conjunctival transmission of SARS-CoV-2 has not been conclusively elucidated and would have a significant [8, 9] ). The German version of this article can be found under https://doi.org/10.1007/s00347-020-01150-1. impact on public health. For example, a handful of studies postulate that SARS-CoV-2 can be transmitted via the mucous membranes, including the conjunctiva [2, 3] , and that all ophthalmologists are at increased risk and should therefore wear protective eyewear when examining suspected cases [14] . Like SARS-CoV, SARS-CoV-2 uses the membrane-bound angiotensin-converting enzyme 2 (ACE2) and the membrane-bound serine protease TMPRSS2 to enter the host cell [7] . During this process, SARS-CoV-2 binds to ACE2 with its spike (S) glycoprotein and is able to fuse with the host membrane via TMPRSS2mediated proteolytic activation (. Fig. 1 ; [7, 16] ies have investigated the expression of ACE2 in a variety of human tissues and, in addition to expression in lung tissue, demonstrated marked expression of the receptor in stomach, colon, liver, and kidney tissue [15, 23] . This highlights the susceptibility of a wide variety of tissues to SARS-CoV-2 infection and explains the clinical observation of possible multiple organ involvement in SARS-CoV-2 infection [19] . It has not yet been definitively elucidated whether ocular surface cells express ACE2 or TMPRSS2 and are therefore susceptible to SARS-CoV-2 infection. There are three fundamental questions related to the possibility of virus transmission via the ocular surface. Can SARS-CoV-2 infect the conjunctiva and replicate there? Recent studies describe subjective ocular symptoms in 7% of all COVID-19 patients [22] and signs of conjunctivitis in approximately 1% [5] . However, these observations were only rarely based on ophthalmological examinations and the inclusion criteria were heterogeneous [10] . In addition, the cited studies did not investigate adequate control cohorts and it is unclear whether the symptoms were caused by SARS-CoV-2 or whether they were an epiphenomenon unrelated to SARS-CoV-2, e.g., resulting from intensive care treatment. There is also controversyas towhetherocularsurface cells, e.g., conjunctival epithelial cells, express ACE2 or TMPRSS2 and are therefore susceptible to SARS-CoV-2 infection. In light of the lack of data, we recently investigated the expression levels of ACE2 and TMPRSS2 in 38 healthy and diseased conjunctival samples [11] . To this end, RNA was isolated from formalin-fixed paraffin-embedded conjunctival samples as previously described, sequenced [12, 17] , and the sequencing data were then bioinformatically analyzed [1] . Whereas the conjunctival samples exhibited significant mRNA expression of the epithelial marker keratin 19, healthy and diseased conjunctival samples showed no relevant expression of the SARS-CoV-2 receptor ACE2 (. Fig. 2a) . Consistent with the virtually undetectable expression of ACE2 at the transcriptional level, we also found negligible ACE2 immunoreactivity in eight healthy conjunctival samples [11] , suggesting the absence of relevant ACE2 protein expression in the conjunctiva (. Fig. 2b) . Furthermore, our data show that the serine protease TMPRSS2 in conjunctival tissue is also not significantly transcribed (. Fig. 2a) . These results argue against an ACE2-mediated conjunctival route of infection for SARS-CoV-2 and are in line with histological investigations conducted on patients having died from COVID-19 that did not detect relevant conjunctivitis [13] . Therefore, SARS-CoV-2 appears to differ from other viruses, such as hepatitis C virus, for which conjunctival infection and transmission are described [8] . Although evidence is lacking to date, it remains to be elucidated whether individual factors such as hypoxia or smoking can trigger ACE2 expression in the conjunctiva [21] . A recent investigation using an ex-vivo model reported the possibility of cell infection, particularly conjunctival stromal cells, by SARS-CoV-2 [9] . However, it remains unclear to what extent this observation can be extrapolated to the in vivo situation. In addition, more aggressive pretreatment of histological conjunctival specimens appears to induce ACE2 immunoreactivity of the conjunctival epithelium; however, the clinical relevance of this is unclear [4] . Other investigations, e.g., on autopsy material from COVID-19-deceased patients, are needed in order to gain insight into actual infectivity and possible sites of virus replication. There are currently no data on SARS-CoV-2 infection of healthy individuals via the tear film. While some data (see above) suggest that SARS-CoV-2 does not infect the conjunctiva, viruses in the tear film could gain access to the nasal mucosa and respiratory tract via the lacrimal drainage system, thereby triggering infection of respiratory epithelial cells. It is currently unclear to what extent rubbing the eyes with contaminated hands can cause infection via the tear film. One can only speculate at present on the importance of virus uptake via the tear ducts compared to direct uptake of virus-containing aerosols via the airways. Due to the protective blinking of the eye and the smaller surfaces, a purely ocular route of SARS-CoV-2 infection likely plays a minor role. However, eye protection appears to be urgently required in the case of close contact with COVID-19 patients or a high risk of exposure, e.g., during intubation or extubation of COVID-19 patients. Ophthalmologe https://doi.org/10.1007/s00347-020-01255-7 © Springer Medizin Verlag GmbH, ein Teil von Springer Nature 2020 What is the significance of the conjunctiva as a potential transmission route for SARS-CoV-2 infections? Due to the low number of COVID-19 patients investigated, the current evidence does not permit a conclusive statement to be made on possible SARS-CoV-2 infection of the conjunctiva. However, since COVID-19 patients only rarely exhibit clinical signs of conjunctivitis and SARS-CoV-2 RNA has been detected only sporadically in tear fluid, conjunctival SARS-CoV-2 infection appears to be unlikely. These clinical observations are supported by basic scientific research that describes low expression of ACE2 and TMPRSS2. This makes conjunctival SARS-CoV-2 transmission via these mediators unlikely-but does not rule out other routes of infection via hitherto unknown receptors. In addition, inoculation of SARS-CoV-2 could occur via tears transporting the virus through the nasolacrimal drainage system to the nose and throat region where cells get infected. Other investigations, e.g., on autopsy material from COVID-19deceased patients, are needed in order to gain insight into actual infectiosity and possible sites of virus replication. Until these possibilities are reliably ruled out, effective prophylactic measures that protect the mouth, nose, and, where necessary, the eyes, should be used by physicians coming into close contact with COVID-19. In routine ophthalmological practice, the risk of infection via respiratory aerosols and close contact to patients during certain ophthalmological examinations is likely to be higher than via the tear film and ocular surface of patients. 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