key: cord-0875390-sqpkw5nt
authors: van de Pol, Alma C.; Wolfs, Tom F.W.; Tacke, Carline E.A.; Uiterwaal, Cuno S.P.; Forster, Johannes; van Loon, Anton M.; Kimpen, Jan L.L.; Rossen, John W.A.; Jansen, Nicolaas J.G.
title: Impact of PCR for respiratory viruses on antibiotic use: Theory and practice
date: 2010-12-30
journal: Pediatr Pulmonol
DOI: 10.1002/ppul.21385
sha: c185afd51440eb8102a60ce7b3858e9a85a24c94
doc_id: 875390
cord_uid: sqpkw5nt

RATIONALE FOR THE STUDY: Real‐time polymerase chain reaction (PCR) for respiratory viruses is more sensitive, yet more expensive, than conventionally used direct immunofluorescence (DIF). We determined the impact of real‐time PCR, additional to DIF, on antibiotic prescription in ventilated children with lower respiratory tract infection (LRTI) at admission to the pediatric intensive care unit (PICU). METHODS: First, a multicenter survey study was performed. Subsequently, in a prospective study, children (≤5 years) with LRTI were tested at admission by DIF and PCR. Positive DIF results were reported at the end of the first working day. PICU physicians reported antibiotic treatment on the second working day. After informing them of the PCR result antibiotic treatment was reevaluated. RESULTS: The multicenter survey study (94 respondents) showed that PCR decreased antibiotic use (P < 0.001). In the prospective study 38 children were included, of which 19 (50%) were DIF positive. Of the 19 DIF negative patients 12 (63%) were treated with antibiotics before revealing the PCR result; the PCR test was positive in 9 out of 12. Revealing PCR results did not alter antibiotic treatment. In 7 DIF negative patients antibiotics not given, the PCR test was positive. CONCLUSION: In contrast to their responses to the survey study, in real‐life PICU physicians did not let their antibiotic prescription be influenced by respiratory real‐time PCR in children ventilated for LRTI. Pediatr. Pulmonol. 2011; 46:428–434. © 2010 Wiley‐Liss, Inc.

Lower respiratory tract infection (LRTI) in young children is a common reason for admission to the pediatric intensive care unit (PICU). 1, 2 The large majority of LRTI in these children is believed to be of viral origin, in which case antibiotic treatment would be unnecessary. 3, 4 However, the differentiation between viral, bacterial or mixed infections is clinically impossible and bacteria can be cultured in a considerable proportion of patients. 5 Therefore, most children ventilated with LRTI are treated empirically with antibiotics. 6, 7 It is unclear whether the introduction of new rapid respiratory viral tests into the work-up of LRTI impacts antibiotic prescription. 8 The limited number of studies evaluating the effect of rapid diagnosis on antibiotic use yielded contradictory results. [9] [10] [11] [12] [13] [14] The impact of viral testing in the PICU setting has not been investigated previously.

Rapid viral testing in the hospital setting has traditionally been performed with direct immunofluorescence tests (DIF). Currently, molecular amplification tests such as real-time polymerase chain reaction (PCR) have become the new golden standard for the detection of respiratory viruses because of their increased sensitivity. 15, 16 Realtime PCR assays have been shown to be quick and reliable in the PICU population, leading to a large increase in diagnostic yield of respiratory viruses. [17] [18] [19] [20] At present, the high costs of PCR assays are considered a disadvantage.

The aim of our study was to evaluate whether a viral diagnosis obtained by the more sensitive real-time PCR as compared to DIF, would impact antibiotic prescription in children admitted with LRTI at the PICU. To answer this question, we first performed a multicenter survey study among PICU physicians. Subsequently, we conducted a 2 years prospective study at our PICU.

A total of six PICU's from three large Dutch medical centers (University Medical Center Utrecht, Amsterdam Medical Center, Erasmus Medical Center Rotterdam) and three large German medical centers (University Medical Center Freiburg, Universitätsklinikum Mainz, Charité Berlin, Germany) participated in this multicenter study. A senior pediatric intensivist of each PICU listed the PICU physicians involved in the care of infants with LRTI at their PICU. Physicians were individually approached by the investigator or the pediatric intensivist for a response.

Questionnaires consisted of five case descriptions, constructed from clinical data from five infants with typical signs of LRTI ventilated at our PICU in 2004. The infants were selected because they had a negative or indeterminate DIF test and a positive real-time PCR result. Characteristics of the five cases are shown in Supplementary Table 1 . After each case description the following question was asked: ''Would you start/continue antibiotic therapy for this child?'' Questions were first asked with the real-time PCR result concealed, and the same questions were repeated after disclosing the real-time PCR result. Anonymity of the respondents was assured. The questionnaire was pilot-tested to assure clarity and coherence, and it was subsequently modified in response to the pilot test results. The order of cases was random in the different questionnaires. Due to the low burden of the survey, ethical approval/informed consent was not needed according to the institutional review board of our hospital.

Ventilated patients under 5-year old admitted with LRTI to the PICU of the Wilhelmina Children's Hospital, who had a DIF test performed as part of routine investigation, were enrolled from October 2006 through October 2008. The Wilhelmina Children's Hospital is a tertiary care medical center with a 16 bed PICU facility. It serves as a referral center for the central part of the Netherlands. Exclusion criteria were asthma exacerbation, immune compromised state, indication for antibiotics other than LRTI, and repeated PICU admission for LRTI during the study period. Baseline clinical data were obtained from medical charts using standardized forms (gender, age, symptom onset, disease severity, antibiotics before admission, preterm birth, underlying illness, duration of ventilation, length of PICU stay, white blood count, and C-reactive protein). Underlying illness was defined as chronic pulmonary disease, congenital heart disease, neurological disease, or gastrointestinal disease. 21 For the assessment of illness severity the oxygenation index (OI) was calculated 12 hr after admission (OI ¼ fraction of inspired oxygen  mean airway pressure  100/partial oxygen pressure).

Nasopharyngeal aspirates were taken by trained nursing staff, the morning of the first working day after admission (working days: Monday-Friday). Specimens were examined with DIF and real-time PCR as previously described. 18, [22] [23] [24] In short, part of the sample was subjected to DIF assays to detect respiratory syncytial virus (RSV), influenza viruses, parainfluenza viruses 1-3, and adenoviruses using Imagen kits (DaKo, Glostrup, Denmark), in accordance with the manufacturer's recommended protocol. Indeterminate DIF results were considered negative for the analyses. The remaining material was used for real-time PCR testing for RSV, influenza viruses, parainfluenza viruses 1-4, adenoviruses, rhinoviruses, coronaviruses, human metapneumovirus, and Mycoplasma pneumoniae. During the second year, the newly ABBREVIATIONS LRTI lower respiratory tract infection PICU pediatric intensive care unit DIF direct immunofluorescence PCR polymerase chain reaction OI oxygenation index RSV respiratory syncytial virus detected human bocavirus was added to the panel. 25, 26 Briefly, after nucleic acid extraction using the MagNA pure LC nucleic acid isolation system (Roche Diagnostics, Basel, Switzerland) amplification was carried out in a 25 ml reaction mixture on a 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA). The positive control for human bocavirus was provided by T. Allander, Karolinska Institute, Stockholm, Sweden.

Internal control viruses were used to monitor for efficient extraction and amplification.

Patient care of enrolled patients was left at the discretion of the attending PICU physician. Results of the DIF assays had a turn-around time of approximately 5 hr, and positive results were reported at the end of the first working day to the attending clinician. Results of the real-time PCR assays had a turn-around time between 16 and 24 hr and the results were reported at the beginning of the second working day to the investigator only. The investigator approached the attending physician before revealing the PCR result, using a questionnaire with the following questions: (1) ''What is the patients' suspected clinical diagnosis?'' (To be answered with checkboxes for viral bronchiolitis, viral pneumonia, and bacterial pneumonia), and (2) ''Is the patient treated with antibiotics?'' Subsequently, the physician was provided with the PCR result. The questions were repeated one hour later. Finally, PICU physicians were asked what the reason was for (dis)continuing antibiotics. Since the prospective study did not involve a change in patient management, ethical approval, and/or informed consent of parents/guardian of infants was not required according to the institutional review board of our hospital.

For statistical analysis of the percentage of antibiotic use before and after disclosure of the real-time PCR results the McNemar test was used.

Ninety-four PICU physicians (20 from Utrecht, 12 from Amsterdam, 16 from Rotterdam, 17 from Freiburg, 12 from Mainz, and 17 from Berlin) completed the questionnaire.

Before disclosure of the real-time PCR result, the percentage of PICU physicians that would treat the five infants with antibiotics was 37-98% (range for the five cases; Fig. 1 ). After disclosure of the real-time PCR result the percentage of physicians that would treat the infants with antibiotics decreased to 19-82% (McNemar test: P < 0.001 for all cases). The absolute reduction was largest for case 1 and 5 (37% and 42%). Although the percentage of antibiotic use pre-PCR test differed between centers, the reduction of antibiotic use following PCR was similar in the different centers.

Thirty-eight children with LRTI were enrolled in this study. Twenty (53%) children were clinically suspected of viral bronchiolitis, 9 (24%) of viral pneumonia, and 9 (24%) of bacterial pneumonia. Demographics and clinical characteristics of the enrolled patients are provided in Table 1 . 9 .0 (5.0) Length of PICU stay in days, median (IQR) 9.5 (6.0) White blood count, median (IQR) 2 8.2 (3.8) C-reactive protein mg/L, median (IQR) 2 27.5 (49.0) IQR, interquartile range; PICU, pediatric intensive care unit; OI, oxygenation index. 1 Oxygenation index 12 hr after admission; available for 23 patients with an arterial oxygen pressure measured at that time. 2 First value after admission. 

Nineteen patients were positive with DIF (50%), all for RSV; no (para)influenza or adenoviruses were detected (Fig. 2) . Real-time PCR confirmed all 19 positive DIF results and detected 7 additional viruses in 6 of them ( Table 2 ). The remaining 19 patients were DIF negative. In 16 of the 19 DIF negative patients real-time PCR detected a total of 20 viruses. In 3 (8%) patients with LRTI no virus could be identified either by DIF or by PCR. Therefore, an infection with one or more viruses was detected in 35 of 38 patients (92%).

In the group of 19 DIF positive patients, 9 (47%) were treated with antibiotics before the real-time PCR result was made available. Antibiotic prescription did not change after revealing the concordant positive real-time PCR results (Fig. 2) .

In the group of 19 DIF negative patients, 12 (63%) patients were treated with antibiotics before the PCR result was made available. The real-time PCR was positive in 9 of these 12 patients. In none of the 9 patients, the positive real-time PCR result prompted the PICU physicians to discontinue antibiotics. In only 1 of these 9 patients the suspected clinical diagnosis was changed by the attending physician from bacterial to viral pneumonia, nevertheless antibiotic treatment was continued. When asked for the reason to continue antibiotics, physicians listed (a) clinical suspicion of bacterial superinfection (ill-appearance, high-ventilator settings, high-C-reactive protein levels; n ¼ 7) and (b) waiting for the final result of the bacterial cultures (n ¼ 2). Finally, in the 7 DIF negative patients that were not treated with antibiotics, a negative real-time PCR result could have prompted physicians to start antibiotics. However, all 7 patients were found to be positive by real-time PCR for respiratory viruses; that is, real-time PCR confirmed the clinical suspicion of a viral infection and no antibiotics were started.

In summary, 21 (55%) of the included 38 patients were treated with antibiotics and this number was not influenced by the real-time PCR results. Of the 29 patients that were believed by the PICU physician to have a viral LRTI (viral bronchiolitis or viral pneumonia), 12 (41%) had antibiotics prescribed.

The present study investigated the impact of respiratory virus real-time PCR, in addition to DIF, on antibiotic prescription in ventilated children with LRTI at the PICU, first in a survey study and then in a prospective study. Although physicians indicated that real-time PCR would have an effect on antibiotic use in the survey study, this effect was not found in practice in the prospective study. Conventional rapid viral tests such as antigen detection by DIF are now being replaced by more sensitive, molecular assays such as real-time PCR. This has led to an increase in the detection of viruses, 18,27-29 with a substantial increase in costs. 22 To determine if real-time PCR theoretically could influence daily practice at the PICU, first a multicenter survey study was performed. Since the survey study indicated that the availability of PCR result would reduce antibiotic use, a 2 years prospective study was undertaken at a single center to test the impact of real-time PCR in real-life.

Three limitations of our study deserve further discussion. First, the number of evaluated PICU children in the prospective study was relatively small. However, realtime PCR did not influence antibiotic prescription in one single patient. Therefore, we believe that the inclusion of additional patients is not likely to influence our observation that the impact of real-time PCR is minor. Second, the fact that physicians were aware of the study may have influenced their decision on antibiotic prescription. However, this would probably have led to an overestimation of the effect of real-time PCR results on antibiotic use which would only make the conclusion of our study stronger. Third, the turn around time of real-time PCR in our study was between 16 and 24 hr and PCR was performed on working days, so the results were available only at the second working day. Possibly, if results had been available earlier this could have had more effect on antibiotic prescription practices (physicians will probably be more reluctant to stop antibiotic treatment than not to start it). However, we aimed to evaluate the impact of real-time PCR in daily practice, and in most hospitals both DIF and real-time PCR are only performed on working days (i.e., Monday-Friday) with the same turn around times for both tests. 30 In agreement with previous studies we showed that realtime PCR increased the diagnostic yield considerably when compared to DIF. The proportion of virus-infected children increased from 50% (DIF) to 92% (real-time PCR), and the number of viruses from a total of 19 (all RSV) to 46 (8 different types). This increased diagnostic yield was not, however, accompanied by a decrease in antibiotic prescription. This finding is in contrast with our survey study in which real-time PCR decreased the percentage of antibiotic description by 16-42% (absolute reduction; P < 0.001 for all cases). Apparently, the treatment of paper patients is not the same as treating patients in daily PICU practice, in which physicians seem to prefer to stay on the safe side. In daily practice, physicians seem to make clinical decisions on other grounds than results from diagnostic investigations. Reasons for not stopping antibiotic treatment in PCR positive patients were the clinical suspicion of a (secondary) bacterial super infection, and the fact that the results of bacterial cultures were still unknown. Additional to the lack of effect of real-time PCR on antibiotic use found in this study, one may wonder what the effect of any viral test such as DIF may be, since a considerable proportion of patients (47%) received antibiotics regardless of a positive DIF result.

Previous studies on the impact of rapid viral testing on antibiotic use in children have been published. Our results are in agreement with Doan et al. 10 and Poehling et al., 12 who did not find a reduction in antibiotic prescription in children randomized to have rapid testing performed at the emergency department. Our results are in contrast with studies from Bonner et al., 9 Noyola and Demmler, 11 Sharma et al., 13 and Woo et al., 14 who found that physicians prescribed less antibiotics in patient groups tested with a rapid viral test. These studies were all performed at the emergency department or at pediatric wards, and not at the PICU, and assessed different age groups. It can be expected that physicians in a PICU setting are more reluctant to withhold antibiotic treatment in the severely ill population with LRTI who frequently has underlying diseases. This is Pediatric Pulmonology Direct immunofluorescence (DIF) was performed for respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses. 3 The DIF positive patients were all positive for respiratory syncytial virus. reflected in our population by the finding that in the 38 ventilated LTRI patients 21 children started with antibiotics at admission (55%) and antibiotic prescription was not changed in any of the groups, not even in the 9 children with a positive PCR and negative DIF.

In addition, virtually all LRTI patients at our PICU (35 out of 38; 92%) were found to be positive by real-time PCR for one or more viruses. This means that in our PICU population virus positivity does not help clinicians anymore to differentiate between those patients who qualify for antibiotics from those who could safely be treated without antibiotics. Therefore, we propose that future real-time PCR studies should be directed to settings were not all patients are found to harbor viruses, such as the population of children with less severe respiratory tract infection in the community. 10 Limiting antibiotic prescriptions in patients with viral LRTI is not the only objective of performing respiratory real-time PCR assays. Real-time PCR is valuable as a very sensitive tool to further explore the clinical presentation and impact of respiratory viruses as well as their epidemiology and it will become an even more important diagnostic tool when antiviral treatments become available. However, when real-time PCR in LRTI patients at the PICU does not affect daily practice, this test could be left out of the diagnostic work-up and costs could be saved.

In conclusion, in daily practice real-time PCR, in addition to DIF testing, has no impact on the prescription of antibiotics in ventilated LRTI patients at the PICU. This is in contrast to the response of physicians on paper case patients in a theoretical survey. This study shows a discrepancy between theoretical considerations and daily practice at the pediatric intensive care. In daily practice physicians seemed reluctant to stop antibiotic prescriptions in the severely ill population of LRTI patients at the PICU.

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