key: cord-0899953-8milanir authors: Haak, Bastiaan W; Brands, Xanthe; Davids, Mark; Peters-Sengers, Hessel; Kullberg, Robert F J; van Houdt, Robin; Hugenholtz, Floor; Faber, Daniël R; Zaaijer, Hans L; Scicluna, Brendon P; van der Poll, Tom; Joost Wiersinga, W title: Bacterial and Viral Respiratory Tract Microbiota and Host Characteristics in Adults With Lower Respiratory Tract Infections: A Case-Control Study date: 2021-06-22 journal: Clin Infect Dis DOI: 10.1093/cid/ciab568 sha: 49fd7a6d4772c9f17b8c782a874fdd9c46d3ea4b doc_id: 899953 cord_uid: 8milanir BACKGROUND: Viruses and bacteria from the nasopharynx are capable of causing community-acquired pneumonia (CAP), which can be difficult to diagnose. We aimed to investigate whether shifts in the composition of these nasopharyngeal microbial communities can be used as diagnostic biomarkers for CAP in adults. METHODS: We collected nasopharyngeal swabs from adult CAP patients and controls without infection in a prospective multicenter case-control study design. We generated bacterial and viral profiles using 16S ribosomal RNA gene sequencing and multiplex polymerase chain reaction (PCR), respectively. Bacterial, viral, and clinical data were subsequently used as inputs for extremely randomized trees classification models aiming to distinguish subjects with CAP from healthy controls. RESULTS: We enrolled 117 cases and 48 control subjects. Cases displayed significant beta diversity differences in nasopharyngeal microbiota (P = .016, R(2) = .01) compared to healthy controls. Our extremely randomized trees classification models accurately discriminated CAP caused by bacteria (area under the curve [AUC] .83), viruses (AUC .95) or mixed origin (AUC .81) from healthy control subjects. We validated this approach using a dataset of nasopharyngeal samples from 140 influenza patients and 38 controls, which yielded highly accurate (AUC .93) separation between cases and controls. CONCLUSIONS: Relative proportions of different bacteria and viruses in the nasopharynx can be leveraged to diagnose CAP and identify etiologic agent(s) in adult patients. Such data can inform the development of a microbiota-based diagnostic panel used to identify CAP patients and causative agents from nasopharyngeal samples, potentially improving diagnostic specificity, efficiency, and antimicrobial stewardship practices. Community-acquired pneumonia (CAP) is the leading cause of hospitalization and death worldwide [1] [2] [3] . The diagnosis of CAP can often be challenging for clinicians, as symptoms of acute heart failure, chronic obstructive pulmonary disease (COPD), or pulmonary embolism may mimic the presentation of the disease [1] . In addition, patients with viral and bacterial lower respiratory tract infections clinically present similarly, and a confirmed microbiological diagnosis is only obtained in approximately half of all cases [1, 4] . An increasing amount of research has focused on studying the composition and function of commensal micro-organisms in the nasopharynx, which has shown to be an ecological proxy for the lower airway microbiota [5] [6] [7] . Although lower in total biomass compared to the gastrointestinal tract, the upper respiratory tract harbors a surprisingly diverse and stable ecosystem of bacteria, viruses, and fungi. Common respiratory pathogens, such as Streptococcus pneumoniae and Haemophilus influenzae are frequently identified in the healthy nasopharynx, yet a balanced community of other commensal bacteria prevents the overgrowth of these pathobionts [8, 9] . However, studies have shown that a loss of these mechanisms of protection by commensal bacteria, otherwise known as colonization resistance, facilitates the enrichment of single bacterial taxa, ultimately leading to respiratory infections [10] [11] [12] [13] [14] [15] . The observed relationship between the loss of colonization resistance and respiratory infections has fueled the hypothesis that shifts in nasopharyngeal bacterial communities could potentially be employed to facilitate the diagnosis of CAP. A recent matched case-control study in neonates strengthened this hypothesis by demonstrating that a classifier model based on nasopharyngeal bacterial and viral communities can accurately identify lower respiratory tract infections [5] . These findings could have implications for future treatment protocols, potentially leading to a reduction in the inappropriate use of antibiotics and, although currently underexplored, are therefore of equal interest to adult CAP patients [16] . However, most studies have limited their scope to identifying the absence or presence of a single nasopharyngeal bacterial species, which does not account for the role that shifts in bacterial communities as a whole could play in the acquisition of CAP [8] . Therefore, this study aimed to investigate if shifts in bacterial and viral communities of the nasopharynx can aid in diagnosis of CAP in adults who present to the hospital. Details of recruitment have been published previously [17] . In brief, consecutive patients older than 18 years admitted to the Amsterdam UMC, location Academic Medical Center (AMC) or BovenIJ hospital in the Netherlands during the influenza seasons (October 2016 to June 2017 and October 2017 to June 2018) were screened by trained research physicians. Patients were included if they were admitted with a clinical suspicion of a community-acquired pneumonia. Patients exposed to antibiotics within 48 hours prior to hospital admission, with the clinical suspicion of an aspiration pneumonia or a hospitalassociated pneumonia were excluded. Subjects of comparable age and sex, who presented for periodical control of cardiovascular management, diabetes care or cancer follow up at the outpatient clinic of the Amsterdam UMC, location AMC, served as controls without acute infection. Nasopharyngeal swabs in Universal Transport Medium (UTM™, Copan) were taken within 24 hours of hospital admission and 1 month thereafter and stored immediately at −80°C. Clinical data and host characteristics were retrieved from electronic medical records and standardized case report forms. We also collected microbiological data regarding causative pathogens (based on a combination of viral nasal/throat swab polymerase chain reaction [PCR], urine antigen tests, blood cultures, and sputum cultures). Microbiological results were deemed to be clinically relevant if the physician caring for the patient deemed an infection was present and elicited a treatment plan based on these findings. Written informed consent was obtained from all eligible participants, or their legal representatives. The study protocol was approved by the local institutional review boards (ref number NL57847.018.16). A detailed methodology of the bacterial sequencing procedure is described in the Supplementary Materials and in prior publications of our group [18] . In short, 16S rRNA gene amplicons were generated using a single step PCR protocol targeting the V3-V4 region. The libraries were sequenced using a MiSeq platform using V3 chemistry with 2 × 251 cycles. amplified sequence variants (ASVs) were inferred for each sample individually with a minimum abundance of 4 reads [19] . Previously detected contaminating sequences, identified using negative controls and the decontam package, were removed [20] . Nasopharyngeal viral communities were analyzed by multiplex real-time PCR (RespiFinder SMARTfast 22, Maastricht, Netherlands). Statistical analysis was performed in R (Version 3.6.1, Vienna, Austria). To assess alpha diversity and richness, we calculated the Inverse Simpson Index and Observed Taxa Richness index with the phyloseq package [21] . Data were not normally distributed and were therefore analyzed using either a Wilcoxon rank sum or Kruskal-Wallis test. Beta diversity was assessed using the weighted and unweighted UniFrac distance metrics, and differences among groups were tested using PERMANOVA as implemented in the vegan package. DESeq2 analysis was used to identify differentially abundant bacterial genera [22] . Finally, extremely randomized trees classification models were used to assess the value of a combination of clinical variables and nasopharyngeal bacterial and viral communities to distinguish CAP of bacterial, viral, and mixed etiology from health [23] . Extremely randomized trees classification models are considered one of the best models to identify bacterial taxa associated with disease [24, 25] . The relative abundance of the top 40 nasopharyngeal bacterial genera, viral presence, and host characteristics (for details see Supplementary Table 1 ) were used as input for the models. We performed 100 iterations of 5-fold cross validation on 75% of each of the data sets, with subsequent testing on the remaining 25% of the samples, and assessed the performance of these classifiers by calculating the mean area under the receiver operating characteristic curve (AUC-ROC) of all 100 shuffles. The models were implemented in Python (v. 3.7.4) using numpy (v. 1.16.4), pandas (v. .25.1), and scikit-learn (v. .21.2) packages. We validated this approach using a dataset of publicly available 16S rRNA V4 sequences (accession number SRP132207), of nasopharyngeal samples from 140 patients with influenza A virus admitted at New York Presbyterian Hospital and 38 healthy controls [12] . In total, 117 cases and 48 control subjects were enrolled in this study (CONSORT flow diagram is provided in Supplementary Figure 1 ). Median age of cases (69.0 years; interquartile range [IQR] 60.0-78.0) and controls (70.5 years; IQR 63.8-75.0) were similar. Demographic characteristics, dietary habits, prior antibiotic exposure, and comorbidities (diabetes, cardiovascular disease, malignancy, gastrointestinal disease, and/or chronic renal disease) were comparable between CAP patients and controls. However, cases had a lower body mass index (P = .014) and a higher prevalence of COPD (P = .004) and immunocompromised status (P = .022) compared to controls ( Table 1 ). The median pneumonia severity index (PSI) class [26] at admission was 4 (IQR 3-4). Intensive care unit admission was required for 9 CAP patients (7.8%), and 28-day mortality was 4.4%. Blood cultures, sputum cultures, and urine antigen tests were obtained in 109 (93.2%), 68 (58.1%), and 73 (62.4%) patients, respectively. Viral data were available for all CAP cases and 47 controls (97.9%). A causative pathogen was identified in 68 patients (59.0%), with 12 patients (10.3%) having copathogen infection. Nineteen patients (16.2%) were infected by S. pneumoniae, 11 patients (9.4%) by H. influenzae, and 5 by Staphylococcus aureus (4.3%). Thirty-seven patients (31.6%) were diagnosed with a respiratory virus, of which 13 (17.9%) were attributed to influenza A or influenza B virus; other prevalent viruses were rhinovirus (10 patients), parainfluenza virus 1-4 (7 patients), and coronavirus NL63 (5 patients) ( Figure 1 ). Two asymptomatic healthy control subjects displayed colonization with a respiratory virus-one with human metapneumovirus (hMPV) and one with rhinovirus. In addition, 16S rRNA gene sequencing of nasopharyngeal samples yielded 11 658 346 high-quality reads (average 45 187 per sample, range 2181-135 753), classified into 397 amplified sequence variants (ASVs). We first investigated the microbiota diversity profiles of the nasopharyngeal samples of control subjects and cases at admission and follow-up. Inverse Simpson diversity and observed Taxa richness indexes were comparable between all groups (Figure 2A , 2B). Unweighted UniFrac beta diversity of the nasopharyngeal microbiota of cases at admission differed significantly from controls (P = .016, R 2 = .01; Immunosuppressive disease was defined as clinically suspected or proven immunodeficiency, the use of immunosuppressive therapy or immunomodulating medication in the past 3 months, including chemotherapy, or the use of more than 10 mg prednisone or equivalent each day for the past 3 months. Figure 2C ), whereas the weighted UniFrac remained comparable between all groups ( Figure 2D ). These findings are supported by the large heterogeneity of nasopharyngeal microbiota composition among all study participants, with varying degrees of nasopharyngeal domination of the genera Corynebacterium, Staphylococcus, and Dolosigranulum (Supplementary Figure 2) . Although it has been shown that COPD is associated with airway microbiota alterations [27] , we found no interindividual dissimilarities in nasopharyngeal microbiota composition and diversity between cases at admission with and without COPD (Supplementary Figure 3) . Next, we verified if elevated nasopharyngeal relative abundances (proportion of total 16s rRNA reads) of common causative CAP agents corresponded with detection in blood cultures, sputum cultures, and urinary antigen tests. We observed that at admission, cases with a confirmed H. influenzae and S. pneumoniae pneumonia harbored higher nasopharyngeal relative abundances of Haemophilus and Streptococcus species, respectively, in comparison to cases with other causative pathogens and controls (P = .002 and P = .0099, Figure 3 ). These findings were independent of the microbial detection method by which the causative pathogen was identified (Supplementary Figure 4) . Nasopharyngeal samples collected following antimicrobial treatment (1 month following admission for CAP) displayed normalized abundances of the corresponding pathogens. This infers that the nasopharynx acts as a proxy of clinically meaningful lower respiratory tract infections. Given these observations, we aimed to explore if nasopharyngeal bacterial community structures, rather than single bacterial taxa, were capable of distinguishing CAP patients at hospital admission from controls. We employed extremely randomized trees classification models using the relative abundance of the top 40 nasopharyngeal bacterial genera, viral presence and clinical variables (depicted in Supplementary Table 1 ) as input. The accuracy of the combined use of these three parameters on the classification of CAP versus health was high, with a mean cross validation AUC of .81 (standard deviation [SD] ± .05; Figure 4A ). Important bacterial discriminatory features of pneumonia were, among others, a high abundance of Haemophilus, Streptococcus, Actinomyces, and Curvibacter in the nasopharynx, and low abundance of several nasopharyngeal commensals, such as Corynebacterium, Cutibacterium, and Lawsonella ( Figure 4B ). Most of these genera were among the most differentially abundant features between cases and controls (Supplementary Figure 5) . Clinical discriminators of CAP were prior antibiotic treatment in the past 3 months, COPD, low body mass index (BMI), immunosuppressive disease, and diabetes ( Figure 4B ). This combined classification system outperformed the models based on bacterial microbiota (AUC .71 ± .08), viral microbiota (AUC .63 ± .04), and clinical characteristics alone (AUC .71 SD ± .06) (Supplementary Figure 6) . Separate models for isolated bacterial CAP (33 cases) and viral CAP (27 cases) showed even higher accuracy, with an AUC of .83 (SD ± .07) and .95 (SD ± .04), respectively ( Figure 4A ). Of interest, most discriminating factors for bacterial CAP ( Figure 4C ), specifically the absence of commensal nasopharyngeal communities, such as Corynebacterium, Staphylococcus, Cutibacterium, and Lawsonella, were similar to the mixed analysis, with viral pathogens dominating the discriminatory power of the viral CAP model ( Figure 4D ). Given the discriminatory power of these communities in distinguishing CAP from health, we validated our approach in a recently published data set of hospitalized patients with influenza A infection and controls from New York, USA [12] . In correspondence with the results obtained in our data set, we observed that the use of nasopharyngeal bacterial community structures alone allowed for excellent distinguishing capacity between influenza patients and control subjects with a mean holdout cross validation AUC of .93 (SD ± .04; Supplementary Figure 7 ). Combined, these findings show that lower respiratory tract infections are associated with consistent shifts in nasopharyngeal community compositions. Such data can inform the development of a microbiota-based diagnostic panel used to identify CAP patients and causative agents from nasopharyngeal samples. DISCUSSION We demonstrate that CAP in adults can be robustly differentiated from health using a small set of nasopharyngeal bacteria, viruses, and clinical variables. This study sheds light on the complexity of the composition of nasopharyngeal microbes during infections, as direct comparison of the microbiota between cases and controls revealed only subtle changes. Specifically, we observed no differences in alpha diversity and richness of nasopharyngeal communities of patients with CAP and controls in this study. In addition, CAP patients displayed small but significant beta diversity differences that were characterized by a higher prevalence of pathogenic bacterial taxa, such as S. pneumoniae and H. influenzae. The observed differences were not driven by altered exposure to antibiotics or altered dietary habits, as these exposures were similar between patients and controls. Patients with microbiologically confirmed S. pneumoniae or H. influenzae infection displayed increased abundance of the corresponding pathogens in the nasopharynx, which is in line with the hypothesis that the nasopharynx can indeed be considered an important source of these microorganisms in respiratory infections [5, 14, 15] . However, a large proportion of CAP patients displayed low levels of these pathogens, indicating that no one-size-fits-all community composition exists during CAP. In support of this theory, a recent human challenge model with influenza suggests that specific baseline microbiota communities, rather than single bacterial species alone, might be most relevant in controlling colonization and spread of S. pneumoniae [9] . Given these observations, we aimed to investigate if shifts beyond microbiota diversity metrics and single bacterial taxa comparisons could be employed to discriminate CAP from health. Despite the observed heterogeneity in both clinical variables and causative agents underlying the disease, our model was capable of robustly discriminating health and disease. The community structures were driven by alterations in the core microbial taxa Staphylococcus, Dolosigranulum, Cutibacterium, and Corynebacterium as well as rare and low abundant taxa, such as Paracoccus and Gemella, although the abundance of causative pathogens attributed to a lesser extent to the distinguishing capacity of the model. Upon validation of our approach in a publicly available dataset of nasopharyngeal 16S rRNA sequences [12] , the distinguishing capacity of Influenza A virus infection based on nasopharyngeal bacterial markers was nearly perfect. The discrepancy in performance of both classification models could potentially be explained by the homogeneity of the validation cohort, which only consisted of patients infected with a single pathogen, compared to the heterogeneity of both viral and bacterial pathogens of the original cohort. However, the distinguishing capacity of the classification model remained robust even in a setting of CAP with a wide variety of viral, bacterial, and unknown-causative pathogens. Given the rapid decrease in sequencing costs and time in recent years [28] , our findings in adults and those of others in children [5] indicate that microbiota-targeted tools could improve the diagnostic specificity and efficiency of identifying respiratory infections. Of interest, a proof-of-principle study using rapid microbiota sequencing with MinION technology in adult intensive care unit patients with pneumonia has shown that such diagnostic tools hold promise and could be clinically applied [16, 29] . There are several potential explanations for the observed shifts in nasopharyngeal microbiota composition during CAP. First, the changes observed in the nasopharyngeal microbiome may precede and contribute to increased susceptibility to infection. For example, recent preclinical evidence shows that mice display larger perturbations of the upper respiratory microbiome with Relative feature importance is calculated as the decrease in node impurity weighted by the probability of reaching that node. Node probability is depicted as a percentage, which can be calculated by the number of samples that reach the node, divided by the total number of samples. The higher the value, the more important the feature. Abbreviations: AUC, area under the curve; CAP, community-acquired pneumonia; COPD, chronic obstructive pulmonary disease; ROC, receiver operating characteristic. age, leading to an enrichment and increased risk of infection by S. pneumoniae [30] . A recent cross-sectional study investigating the upper respiratory tract microbiota revealed that increasing age was associated with a loss of common nasopharyngeal commensals Corynebacterium, Dolosigranulum, Staphylococcus, and Cutibacterium, and a relative enrichment of oral flora, such as Actinomyces, which could predispose to reduced colonization resistance and an increased risk of infections [10] . Longitudinal studies in neonates have shown that early airway colonization with pathogenic bacteria occurred prior to the microbiological detection of (viral) pathogens and acute symptoms, and this overgrowth is therefore considered an increased risk for respiratory infections [13] [14] [15] . In our data, this mode of action is supported by the finding that prior antibiotic use is a strong marker for bacterial CAP. Second, the host response induced by the respiratory infection itself allows for selective enrichment of certain bacteria, therefore leading to an altered composition of the nasopharyngeal microbiota. For example, it has been shown that separate mechanisms involving Th17-cell responses and interferonlambda production disrupt the nasopharyngeal microbiome and predispose the occurrence of S. pneumoniae and S. aureus colonization [31, 32] . Support for these mechanisms has also been demonstrated in the context of viral infection, as 2 recent human rhinovirus challenge models and one study investigating the nasopharyngeal microbiome during coronavirus disease 2019 (COVID-19) pneumonia showed the potential of disturbing bacterial nasopharyngeal communities, further elucidating the close relationship between bacterial and viral kingdoms [33] [34] [35] . Longitudinal observational studies should further elucidate the role of the observed communities in the development of respiratory infections. Regardless of the directionality of the observed shifts, this study adds to the growing body of work that supports a common pathway for the development of viral and bacterial CAP, in which a loss of microbial colonization resistance and individual host factors are more important drivers of disease than the characteristics of individual pathogens [5, 36] . Strengths of this study include the use of validated classification models and the prospective case-control design. A limitation of this investigation is the use of amplicon-based 16S rRNA gene sequencing, which provides limited taxonomic resolution at species level. In addition, PCR bias did not allow us to directly validate the yielded models from our dataset (which sequenced the V3-V4 region of the 16S rRNA gene) to the validation data set (which only addressed the V4 region) [37] . It is warranted to increase the taxonomic resolution and use reproducible metagenomic sequencing based tools in order to validate our approach across multiple longitudinal cohorts and clinical settings. In conclusion, this study shows that the relative proportions of bacteria and viruses-based on 16S rRNA gene sequencing and multiplex PCR-can be leveraged to diagnose CAP and identify etiologic agents in adult patients. Future studies are warranted to validate these classification models in the clinical setting, specifically aiming to distinguish patients with respiratory infections from those presenting with heart failure, pulmonary embolism or inflammatory pneumonitis. In addition, further study is warranted to validate if nasopharyngeal communities could discriminate bacterial CAP from viral etiologies with sufficient sensitivity and specificity. If addressed, such data can inform the development of a microbiota-based diagnostic panel used to identify CAP patients and causative agents from nasopharyngeal samples, potentially improving diagnostic specificity, efficiency, and antimicrobial stewardship practices. Community-acquired pneumonia CDC EPIC Study Team. Communityacquired pneumonia requiring hospitalization among U.S. adults World Health Organization. The top 10 causes of death Clinician-targeted interventions to influence antibiotic prescribing behaviour for acute respiratory infections in primary care: an overview of systematic reviews Bacterial and viral respiratory tract microbiota and host characteristics in children with lower respiratory tract infections: a matched case-control study Dynamics of bacterial colonization with Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis during symptomatic and asymptomatic viral upper respiratory tract infection Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia The microbiota of the respiratory tract: gatekeeper to respiratory health Interaction between the nasal microbiota and S. pneumoniae in the context of live-attenuated influenza vaccine Dysbiosis of upper respiratory tract microbiota in elderly pneumonia patients The nasopharyngeal microbiota in patients with viral respiratory tract infections is enriched in bacterial pathogens Microbial composition of the human nasopharynx varies according to influenza virus type and vaccination status The infant nasopharyngeal microbiome impacts severity of lower respiratory infection and risk of asthma development Airway microbiota dynamics uncover a critical window for interplay of pathogenic bacteria and allergy in childhood respiratory disease Early respiratory microbiota composition determines bacterial succession patterns and respiratory health in children Rapid pathogen identification in bacterial pneumonia using real-time metagenomics Concurrent immune suppression and hyperinflammation in patients with community-acquired pneumonia Long-term impact of oral vancomycin, ciprofloxacin and metronidazole on the gut microbiota in healthy humans DADA2: High-resolution sample inference from Illumina amplicon data Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 Extremely randomized trees Best practices for analysing microbiomes Machine learning performance in a microbial molecular autopsy context: a cross-sectional postmortem human population study A prediction rule to identify low-risk patients with community-acquired pneumonia Community dynamics and the lower airway microbiota in stable chronic obstructive pulmonary disease, smokers and healthy non-smokers Exploring the human microbiome: the potential future role of next-generation sequencing in disease diagnosis and treatment Towards an ecology of the lung: new conceptual models of pulmonary microbiology and pneumonia pathogenesis Respiratory microbiota dynamics following Streptococcus pneumoniae acquisition in young and elderly mice IL-17 signalling restructures the nasal microbiome and drives dynamic changes following Streptococcus pneumoniae colonization Lambda interferon restructures the nasal microbiome and increases susceptibility to Staphylococcus aureus superinfection Differences in the nasopharyngeal microbiome during acute respiratory tract infection with human rhinovirus and respiratory syncytial virus in infancy Changes in microbiota during experimental human Rhinovirus infection Metagenomic next-generation sequencing of nasopharyngeal specimens collected from confirmed and suspect COVID-19 patients Integrating host response and unbiased microbe detection for lower respiratory tract infection diagnosis in critically ill adults Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis Acknowledgments. The authors thank all patients and their families who participated in the study. They also thank Jorn Hartman, Stijn Klarenbeek, and Rosan van der Lee for their help with the workup of the nasopharyngeal microbiota samples.Availability of data. Raw sequencing data (bacterial ASVs) have been submitted to the European Nucleotide Archive (accession number PRJEB42265).Financial support. This work was supported by the Netherlands Organization for Scientific Research (VIDI grant number 91716475 to W. J. W.), and the Netherlands Organization for Health Research and Development (ZonMW grant number 50-53000-98-139 to X. B.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Potential conflicts of interests. W. J. W. served as consultant for GSK (DSMB committee), fees paid to institution. All other authors report no potential conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed. Supplementary materials are available at Clinical Infectious Diseases online. Consisting of data provided by the authors to benefit the reader, the posted materials are not copyedited and are the sole responsibility of the authors, so questions comments should be addressed to the corresponding author.