key: cord-0912799-uu0je9zx
authors: Hamley, Ian W.
title: Lipopeptides for Vaccine Development
date: 2021-07-06
journal: Bioconjug Chem
DOI: 10.1021/acs.bioconjchem.1c00258
sha: df7f71985269c2056da5b42701c03bb4e8fef906
doc_id: 912799
cord_uid: uu0je9zx

[Image: see text] The development of lipopeptides (lipidated peptides) for vaccines is discussed, including their role as antigens and/or adjuvants. Distinct classes of lipopeptide architectures are covered including simple linear and ligated constructs and lipid core peptides. The design, synthesis, and immunological responses of the important class of glycerol-based Toll-like receptor agonist lipopeptides such as Pam(3)CSK(4), which contains three palmitoyl chains and a CSK(4) hexapeptide sequence, and many derivatives of this model immunogenic compound are also reviewed. Self-assembled lipopeptide structures including spherical and worm-like micelles that have been shown to act as vaccine agents are also described. The work discussed includes examples of lipopeptides developed with model antigens, as well as for immunotherapies to treat many infectious diseases including malaria, influenza, hepatitis, COVID-19, and many others, as well as cancer immunotherapies. Some of these have proceeded to clinical development. The research discussed highlights the huge potential of, and diversity of roles for, lipopeptides in contemporary and future vaccine development.

Vaccination has been a highly successful life-saving method to prevent viral infections, since its development by Jenner in 1796 to treat smallpox, which has now been eradicated. Vaccines based on live, attenuated pathogens have been developed for many diseases; however, for other infections, such vaccines have not been successful or they are ineffective since they fail to provide long-lasting immunity (for example, influenza). In this case, vaccines that use inactivated viruses or other subunits may be created. The current COVID-19 pandemic has focused attention on the impact of viral disease on human society and already vaccines have been developed, remarkably rapidly, based on mRNA technology (the genetic material is delivered in liposomes) or the use of genetically modified (nonhuman) adenovirus carriers (which incorporate spike protein genes), or simply nanoparticle formulations containing virus spike protein subunits as well as whole inactivated viruses. Further information on this is widely available. 1−6 Peptides and lipopeptides are attractive in the development of vaccines, both as antigens and as adjuvants. Peptide and lipopeptide antigens can be developed based on sequences from antigenic proteins, and they can be used to stimulate cell surface receptors in a highly specific manner. Peptides offer advantages in the ease of design and preparation using automated synthesis methods, and they can also be conjugated to lipids and other molecules in the development of adjuvants (or for antigen presentation) in subunit vaccines. Conjugates of peptides and lipids, giving lipidated peptides, termed lipopeptides, are the focus of the current Review which discusses various approaches to the synthesis and application of these molecules, a type of peptide amphiphile. Peptides are relatively inexpensive and safe to produce and can be synthesized at high purity, avoiding contaminants such as lipopolysaccharides that can be present in bioderived protein materials. A further feature of certain classes of peptide-based molecules including lipopeptides is their propensity to self-assemble into nanostructures such as fibrils, micelles, and other structures. 7−13 This property is of interest because it leads to presentation of bioactive peptide motifs at high density, leading potentially to improved antigen and/or adjuvant efficacy, and some examples utilizing this strategy are discussed in this Review. As yet, lipopeptide-based vaccines are not used in clinical practice, although several peptide-based vaccines have reached clinical trials and/or are currently in active development. 14−19 Many vaccines require formulation with adjuvants, which are biomolecules that stimulate immune responses in order to enhance the activity of a vaccine. The roles of lipopeptides as antigens and/or adjuvants is the focus of the current Review, and it includes the important class of self-adjuvanting lipopeptides, which incor-porate both antigen and adjuvant activities. This Review does not consider proteins or lipoproteins and is focused on lipopeptides. Although there is no rigorous distinction between long peptides and proteins, here we consider peptide-based systems with less than about 100 residues.

Potential treatments for cancer also include novel immunotherapies. Cancer immunotherapies include potential vaccines, monoclonal antibodies, T cell transfer therapy including CAR (chimeric antigen receptor) T cell therapy, immune checkpoint inhibitors (which modulate immune response), and immune system modulators such as cytokines (for example, interferons and interleukins). Lipopeptides have potential roles in many of these approaches; in particular, TLR (Toll-like receptor) agonist peptide-based molecules have attracted attention in cancer immunotherapies, as discussed in more detail here, and in a recent review focused on lipopeptides with applications as adjuvants for cancer vaccines. 20 This Review is organized as follows. Section 2 provides a brief overview of the immune system and provides the background on the cellular processes associated with the immune response, as well as introducing key terminology. Section 3 concerns ligated lipopeptides, in which lipid chains and peptides are coupled by ligation methods. Section 4 covers TLR agonist lipopeptides, one of the most intensely researched types of peptide amphiphiles, because some of this class provoke a strong and specific immune response, and indeed such molecules are used as model TLR agonists. Section 5 discusses lipid core peptides which have more complex architectures than those of ligated and TLR agonist lipopeptides, indeed generally offering a multivalent presentation of peptide epitopes. Lipopeptide micelle self-assembled structures relevant to immunotherapies are discussed in section 6. Concluding remarks in section 7 close this Review.

INTRODUCING KEY TERMS The immune system comprises the innate and adaptive systems. The innate immune system relies on macrophages, neutrophils, natural killer cells, dendritic cells, and others. The adaptive immune system is activated by the presentation of antigens by antigen-presenting cells (APCs) of the innate immune system. Antigen presentation involves the binding of antigen to the major histocompatibility complex (MHC), the complex then being transported to the cell surface where it is presented and is capable of recognition by a T cell receptor (TCR). This receptor binds peptides presented by class I or class II major histocompatibility complexes (also known as human leukocyte antigen, HLA, for humans) on APCs (Figure 1 ). Whole antigens are processed by proteolysis by APCs into short peptides (8−11 residues in length for class I MHCs and 11−30 residues in length for class II MHCs), which are presented via MHCs at APC surfaces. 19 TCRs that are specific for particular peptide epitopes then bind the peptide−MHC complexes, and a variety of proteins at the T cell/APC interface orchestrate expansion of clones of the T cells.

There are two types of MHC, one is involved with intracellular peptides from the cytosol (MHC class I), while MHC class II molecules bind peptides in endocytotic vesicles after internalization. The MHC-I/peptide complex activates cytotoxic T cells (T c or killer T cells or cytotoxic T-lymphocyte CTL, a type of white blood cell) via TCRs, these cells also expressing CD8 + co-receptors ( Figure 1 ) (here, CD refers to cluster of differentiation, a class of cell surface glycoproteins that can act as receptors or ligands). On the other hand, presentation of antigens via MHC class II is to CD4 + T cells (T h helper T cells, another type of white blood cell which sends signals to other cells including T c cells) (Figure 1 ). Only specific types of APCs such as dendritic cells, B cells, or macrophages present MHC-II at high levels, so expression of MHC-II molecules is more cell-specific than MHC-I. The innate immune system relies on pattern recognition receptors (PRRs) to detect infecting microbes ( Figure 1 ). PRRs are present in germline cells. They can detect a wide range of pathogens, although they lack the specificity of somatic T and B cells. Most adjuvants are ligands for PRRs. The innate immune response involves the recognition by PRRs of pathogenassociated molecular patterns (PAMPs) which include TLRs, NOD-like receptors (NLRs), C-type lectin agonists (CLRs), RIG-I (retinoic acid-inducible gene I), stimulator of interferon (IFN) genes (STINGs), and others. 21−23 Adjuvants based on these and other PAMPs can enhance the immune response (act as agonists) by interacting with PRRs on antigen-presenting cells. PAMPs elicit specific antigen presentation and cytokine production. The role of TLRs which are a key target for lipopeptides, as discussed later in this Review, has been reviewed extensively. 24−27 PRRs signal through a variety of pathways involving distinct intermediates and key transcriptional factors NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells), and interferon regulatory factors IRF-3 and IRF-7 (IRFs primarily regulate type I interferons, IFNs, in the host after pathogen infection) are activated, leading to the production of cytokines and chemokines that prime and expand the immune response.

The activated adaptive immune system relies on antigenrecognizing species including T cells, B cells, dendritic cells, and antibodies (immunoglobulins). As mentioned above, the adaptive immune system produces T-helper cells which release cytokines to "help" other immune cells. T-Helper cells proliferate into differentiated Th1 cells or Th2 cells ( Figure  1 ). The former lead to a cell-mediated response and the latter to a humoral response which refers to the production of antibodies and antimicrobial peptides and other agents in extracellular fluid (and is also known as antibody-mediated immunity) . The main  effector cells of Th1 immunity are macrophages as well as CD8 +  T cells, IgG B cells, and IFN-γ CD4 + T cells. The main effector  cells for Th2 are leucocytes (white blood cells) including  basophils, eosinophils, and mast cells as well as B cells and IL-4/  IL-5 CD4 + T cells. Th1 cells produce cytokines including INF-γ  (interferon-gamma) and TNF-β (tumor necrosis factor-beta). The Th2 response leads to interleukin cytokines including IL-4, IL-5, IL-6, IL-9, IL-10, and IL-13. Th1-stimulated IFN-γ increases the production of IL-12 by dendritic cells and macrophages.

The activity of a vaccine may be enhanced using adjuvants, and adjuvant development is currently attracting immense interest as a means to substantially boost the performance of vaccines active against a number of diseases. Many vaccines confer humoral immunity, although adjuvants have been/are being developed to stimulate cellular (Th1) immunity. 21 The most effective licensed vaccines elicit persistent T cell and B cell memory as well as long-term antigen-specific antibody responses by plasma cells. 28 Adjuvants are used to increase antibody production or to modulate the adaptive response. They boost the performance of vaccines, enabling a lower dose of antigen and/or a smaller number of required vaccinations. They hence increase the level of immunization within the general population that has been vaccinated and can boost immunity for groups with lowered responsiveness such as older or infant populations. 21 As well as generally boosting the immune response, adjuvants can be used to adjust the nature of the immune response, for example, to change the Th1 versus Th2 response (so-called polarization of helper T cells) or the balance of cytotoxic T cells versus helper CD4 + T cells or T cell memory as well as altering the rate of immune response and its specificity. 21 The type of adjuvant selected will depend on the nature of the desired CD4 + T cell response. Adjuvants should elicit a protective CD8 + response depending on the type of vaccine. Vaccines that cause direct infection of cells including viral carriers or RNA/DNA induce CD8 + immunity through the endogenous class I presentation pathway; however, other vaccines require cross-presentation. 21 Adjuvants have a number of roles including enhanced presentation of antigens and direct stimulation of an immune response using inactivated toxin or virus components or other immune-stimulating molecules such as bacterial lipopolysaccharides or Toll-like receptor agonists including lipopeptides, as discussed in section 4.

Traditionally, alum has been used as a common vaccine adjuvant, and recent reviews discuss other widely used inorganic adjuvants; see, for example, refs 21, 22, and 29. Later developments include oil-in-water emulsions such as Freund's complete adjuvant which contains heat-killed Mycobacterium tuberculosis (incomplete Freund's adjuvant lacks the mycobacteria) or squalene oil-in-water emulsions AS03 or MF59 (squalene is more readily metabolized than paraffin, used in Freund's adjuvants). 21, 22 Alum and emulsion adjuvants are generally considered to have good safety profiles. 21, 30, 31 However, Freund's aduvant contains many innate immune stimulants and can cause side serious effects; indeed, use of both Freund's complete and incomplete adjuvants was discontinued for this reason. 22,31−33 Organic adjuvants such as those based on peptides, proteins, lipids, polysaccharides, and lipopeptides offer scope to tailor more specific immune responses and are the focus of considerable interest in the development of new vaccines. 

A series of lipopeptides (sequences given in ref 36) containing palmitoyl chains at lysine ε-amino groups has been produced as part of an HIV vaccine development program. 36−38 Although the conformation and self-assembly of these lipopeptides has not been examined, they show promise as vaccines, since B and T cell anti-HIV responses were detected in >85% of the vaccinated volunteers after one month, and the research proceeded to clinical trials. 14, 38, 39 In another study, palmitoylation at the lysine residue was also used to increase the immunogenicity of a series of peptides derived from four malaria parasite P. falciparum antigens. 40 Again, although information on peptide and lipopeptide conformation and ordering is not provided, these molecules generate multi-epitopic and long-lasting antigen-specific CD8 + CTL responses in chimpanzees, showing potential also as human vaccines. 40 N-Terminal lipidation (palmitoylation) of the model antigen herpes simplex virus type 1 (HSV-1) gD 1−23 peptide increases uptake and maturation of dendritic cells via TLR2 and triggers Th1-dependent protective immunity. 41 The lipidation was performed by N-terminal attachment of a single N ε -palmitoyl-lysine to the N-terminal lysine ε-amino in the peptide using chemoselective ligation. 41 This method avoids problems with solid phase lipidation methods that arise due to the amphiphilicity of the lipopeptides which can complicate solution separation of the target lipopeptide. 42 The method can be used to prepare mixtures of lipopeptides (demonstrated with peptides based on simian immunodeficiency virus fragments and a Clostridium tetani sequence) and involves the site-specific introduction of a fatty acyl moiety in solution to a mixture of individually prepurified peptides. 42 The lipidation is based on the quasi-stoichiometric and high-yield ligation of a glyoxylyl lipid with hydrazinoacetyl peptides. This method was used to produce the HIV lipopeptide cocktails mentioned above, along with other antigen sequences. 43−45 A modification of this method was used to attach three N-terminal palmitoyl chains (via three K residues) to two peptide epitopes in the development of a herpes simplex virus vaccine for intravaginal delivery via the genital mucosa. 35 The peptide sequence includes CD4 + Th and CD8 + CTL sequences (Scheme 1), and the attachment of one, two, or three palmitoyl chains was motivated by the demonstrated TLR2 agonist properties of related Pam n Cys lipopeptides (discussed in section 4). 35 

Lipoproteins and lipopeptides are important components of the cell wall of both Gram-negative and Gram-positive bacteria. Gram-negative bacterial membranes tend to contain lipopeptides with three lipid chains, whereas Gram-positive cell walls contain lipopeptides bearing two lipid chains. 27 These are heterogeneous in terms of fatty acid chain length and degree of saturation. Lipoproteins derived from Gram-negative bacteria, such as E. coli, 49 Borrelia burgdorferi, 50 Neisseria gonorrheae, 51 Neisseria meningitidis (meningococcus), 52 and Porphyromonas gingivalis, 53 have been shown to be TLR2 agonist triacylated lipoproteins (N. meningitidis lipoproteins are the basis of the vaccine Trumenba 53 ). Lipoproteins from Gram-positive bacteria such as Staphylococcus aureus act on TLR2 and are diacylated derivatives. 54, 55 A number of lipopeptide adjuvants have been developed based on stimulation of TLRs. The core structure with one, two, or three lipid chains linked through a glyceryl cysteine linker to a peptide sequence is shown in Scheme 2, which shows the main peptide sequences that have been used and developed commercially. Peptide-and lipopeptide-based TLR agonist systems for vaccine development have been reviewed. 20, 27, 47, 56, 57 Tripalmitoyl-S-glyceryl cysteine (Pam 3 Cys) is derived from the N-terminal moiety of Braun's lipoprotein that spans the inner and outer membranes of Gram-negative bacteria. On the other hand, dipalmitoyl-S-glyceryl cysteine (Pam 2 Cys) corresponds to the lipid moiety of macrophageactivating lipopeptide 2 isolated from mycoplasma (bacteria which lack cell walls). It has also been reported that Pam 2 Cys is a more potent stimulator of splenocytes and macrophages than Pam 3 Cys. 58 It also has a negligible pro-inflammatory response, as assessed in terms of cytokine (TNF-α, IL-1β, IL-6, and IL-8) release in a study using whole human blood. 59 Pam 3 Cys activates TLR2 to produce a Th2 response, as evidenced by the production of associated effector molecules including IL-13 and IL-1β but not Th1-related cytokines. 60 Other groups found that TLR2 stimulated by Pam 3 CSK 4 promotes Th2 response, 61 as well as that of Th17 which is implicated in several autoimmune conditions. 61−63 The TLR2 activation was found to occur via other TLRs (TLR4 and TLR7/8), since cytokine production in human DCs induced by these TLRs was inhibited by TLR2. 61 These reports seem to be contradicted by another study, in which it is reported that Pam 3 CSK 4 and MALP-2 trigger Th1 cell function via TLR2 but do not stimulate any Th2 cell response. 64 The X-ray crystal structure of the Pam 3 Cys ligand co-crystallized with the TLR1/2 dimer has been reported, 65 as has the crystal structure of Pam 2 CSK 4 with the TLR2/TLR6 dimer. 66 In a pioneering paper, Braun reported that a lipoprotein component of the outer membrane of E. coli is a specific and potent antigen. 67 This group had earlier identified Pam 3 CSSNAK as the N-terminal domain from the murein (peptidoglycan) lipoprotein obtained from the outer cell membrane of E. coli. 68, 69 Shorter fragments of the SSNAK peptide were also isolated, 69 and synthetic lipopeptides bearing these sequences were prepared. All of them were found to be mitogenic and stimulated B lymphocytes into immunoglobulin secretion, as shown by a hemolytic plaque assay. 70, 71 The activity of all lipopeptides bearing different peptide fragments was comparable to that of the native E. coli lipoprotein, although those bearing only cysteine were hardly active. 71 The Pam 3 CSSNAK lipopeptide was shown to also have adjuvant activity, as shown in a study of antibody response in sheep. 72 This group also compared the activity of Pam 3 CSSNA, Pam 3 CSK 4 , Pam 3 CAG, and Pam 3 CS, and all lipopeptides were shown to have excellent adjuvant activity. 73 The lipopeptide Pam 3 CSK 4 was found to be a potent antigen able to fully replace Freund's complete adjuvant (PCS) enhancing immunoglobulin production in mice. 73 Earlier studies on Pam 3 Cys-based peptides with adjuvant or antibody-stimulating properties or self-adjuvating forms have been summarized. 74 Peptides and lipopeptides such as Pam 2 CSK 4 and Pam 3 CSK 4 were developed with enhanced amphiphilicity (compared to the native E. coli murein lipoprotein), with the additional lysine residues conferring solubility. 73 These molecules are agonists of TLR2 in particular and the Pam 3 Cys variants also with TLR1. 20, 58, 59, 75, 76 The peptide conformation and self-assembly properties of the mono-, di-, and tri-palmitoylated CSK 4 lipopeptides were compared using a combination of CD spectroscopy, cryo-TEM, and SAXS. 77 This revealed that PamCSK 4 and Pam 2 CSK 4 form spherical micelles ( Figure  2a ,b) in which the peptide adopts an unordered conformation. In contrast, Pam 3 CSK 4 forms a population of nanotape structures (Figure 2c ), based on β-sheet aggregation (these disorder on heating). 77 The experimental findings were confirmed by later atomistic molecular dynamics computer simulations. 78 Due to the potent immunogenic properties of Pam n Cys lipopeptides, much work has been undertaken to determine the structural features required for activity, as well as efforts to improve the response. Jung and co-workers prepared a range of analogues of Pam 2 CSK 4 with variation in the length of the two alkyl chains and analogues of Pam 3 CSK 4 with different N-acyl groups. 79 They also determined the activity of the synthesized lipopeptides using HEK293 cells transfected with human TLR2 and firefly luciferase reporter gene, measuring the release of IL-8 in the culture medium and NF-κB translocation using luciferase reporter assay. They found that a critical length of more than eight carbons in the two ester chains is required to elicit a biological response, whereas the amide (N-acyl) chain length is less important. 79 Triacylated lipopeptides with short esterbound fatty acids such as PamOct 2 CSSNASK 4 induce no response in TLR1-deficient cells, 80 and Pam 2 CSK 4 and the Cterminally elongated MALP-2 derivative Pam 2 CGNNDESN-ISFKEKSK 4 (MALP2-SK 4 ) induce cell activation in a TLR6independent manner. 81 These molecules can be used as TLRindependent controls in studies of immunogenic response. This group also screened randomized Pam 2 Cys variants (Scheme 3) for B cell proliferation activity using murine splenic lymphocytes. 82 This led to the identification of two lipopeptides, Pam 2 CGQHHM-NH 2 and Pam 2 CSSHHM-NH 2 , with en-hanced activity compared to Pam 3 CSK 4 . As well as the variants shown in Scheme 3, this group also investigated the effect of lipid chain length from C 6 −C 20 in Pam-Cys(Dhp)-SSNASKKKKbased [Dhp: 3,4-dihydropyran] homologues, and the lipopeptide-induced IL-8 release from HEK 293-TLR2 cells was assayed. 83 The activity was found to be significantly reduced for lipopeptides with lipid chain lengths shorter than C 10 . Esterbound oleic acid and/or linoleic acid gave the best results in the IL-8 release assay. 83 As mentioned above, a number of groups have investigated structure−activity relationships in terms of the structure of the S-(2,3-dihydroxypropyl)-L-cysteine linker present in several TLR2 agonist lipopeptides. Scheme 4 summarizes the molecular structures in these studies. 20 Studying MALP-2 (Scheme 2), Takeuchi et al. reported the significantly greater TLR2 activity of the R stereoisomer 4 (Scheme 4) compared to the S version 5, based on the analysis of cytokines, chemokines, or NO released by cells. 84 Substitution of the thioether moiety with either a methylene (6) 85, 86 or oxyether (7) 87 group leads to a loss in TLR2 activity, whereas substitution of S with Se (in 8) causes an upregulation of p38 mitogen-activated kinase (p38MAPK) in neutrophils. 88 The Rcysteinyl form of Pam 2 CSK 4 is also maximally active in reporter Bioconjugate Chemistry pubs.acs.org/bc Review gene assays using human TLR2, as revealed in a study of the role of the highly conserved Cys residue as well as the geometry and stereochemistry of the Cys-Ser dipeptide unit. 87 Changing the length of the spacer between the thioether and the α-center of cysteine (9 containing a penicillamine-based linker or homocysteine-based 10) leads to dramatic reductions in TLR2 activity. 88 Substitution of the ester functionalities for amide (in 11) 89 or ether (in 12) 90 groups in lanthionine scaffolds strongly inhibits TLR2 activity. Compound 13 (corresponding to PamCys, Scheme 2) has pronounced TLR2 agonist activity. 88 However, inversion of the ester bond (in 14 and 15) or extension of the chain between the thioether and ester groups leads (in 16) to a significant reduction in activity. 88 A derivative with a thioethanol bridge in place of the thiogylcerol unit retained TLR2-specific NF-κB induction activity. 88 The role of stereochemistry (R-and S-stereoisomers of the glycerol moiety) has been examined for Pam 3 CSK 4 , and it was found that the R-stereoisomer has greater activity, 74, 91 similar to the case of MALP-2. This was ascribed to enhanced TLR2 triggering due to greater DC activation which stimulates CD8 + T cell responses, in particular higher IL-12 secretion, and upregulates relevant markers for DC maturation. 91 Methylene substitution [to give 2-(palmitoylamino)-6,7-bis(palmitoyloxy)heptanoic acid) (6)] leads to lower activity than Pam 3 CSK 4 at a given concentration, although the maximal activity obtained at significantly higher concentration is similar to that of Pam 3 CSK 4 . 86 The role of stereochemistry was also examined, all four stereoisomers being prepared, the 6S form was active in terms of radiolabeled [ 3 H]thymidine incorporation (a cell proliferation assay) into the DNA of mice splenocytes after stimulation with lipopeptides than the 6R form, with little influence of the other (2S/2R) stereocenter. 86 The authors also noted that these lipopeptides constitute potent macrophage activators with anticancer activity: in particular, the (2S,6S) stereoisomeric form was able to induce tumor cytotoxicity. 86 Further details of the synthesis of the derivatives discussed in this section are provided by Brimble and co-workers. 20, 57 A series of Pam 2 CSK 4 analogues (Scheme 5) were prepared that contain a urea (carbamyl) linker (hence, they were termed UPam) to one lipid chain and a range of substitutions for the serine residue. 92 The motivation for the N-tetradecylcarbamyl substitution was the expectation that immunostimulatory activity would be enhanced, on the basis of modeling of the crystal structure of the X-ray structure of the Pam 3 Cys ligand cocrystallized with the TLR1/2 dimer. 65 Probing DC maturation, the authors observed that UPam derivatives 3−5, 7, 9, and 10 (Scheme 5), which contain small, mostly hydrophobic side chains substituting for the hydroxymethyl of 1 (UPam-Ser), show an increased potency compared to Pam 3 CSK 4 and those containing amino acids with straight alkyl chains of moderate size terminated with a polar functional group (12 and 13) also have enhanced activity (compared to Pam 3 CSK 4 ). In contrast, the derivatives 8, 14, 15, 20, and 22 were found to have minimal activity. 92 Lipopeptide Pam 3 CSK 4 has been investigated as an adjuvant. For example, it can elicit immunogenicity, i.e., CTL responses to Plasmodium berghei circumsporozoite (CS, a parasite that can cause malaria in rodents) peptide epitope SYIPSAEKI either in mixtures or by conjugation of this sequence onto Pam 3 CSK 4 (to give Pam 3 CSK 4 SYIPSAEKI), as assayed using mouse splenocytes. 93 In addition, a conjugate of Pam 3 Cys with poly(ethylene glycol) (PEG) also known as polyoxyethylene (the synthesis of the conjugate being described earlier 94 ) was investigated as part of the range of formulations studied, and this conjugate mixed with CS also generated a CTL response. 93 The influence of the structure of Pam 2 Cys analogues on TLR2 agonist activity prepared as potential adjuvants for cancer vaccines has been examined. 20 The effects of homologation between the two ester functionalities, N-terminal acylation, and acyl group stereochemistry (Scheme 6) were examined in lipopeptides bearing the SK 4 sequence linked to an epitope from the tumor associated NY-ESO-1 protein based on the sequence SLLMWITQC (Scheme 6b). 20 For the bioactivity assays, HEK-Blue-hTLR2 cells were transfected with the TLR2-NF-κB-SEAP reportergene system. This system has been taken forward into commercial development by SapVax. 95 Pam 3 CSK 4 has also been used in the development of an HIV vaccine, by conjugation to the 32-amino-acid group-specific antigen peptide containing at least five CTL epitopes, NPPIPVGEIYKRWIILGLNKIVRM-YSPTSILD. 96 Direct thiol−ene coupling has been used to alkylate CSK 4 with an S-palmitoyl chain at the N-terminus. 97, 98 In addition, as a model vaccine cytomegalovirus (CMV) ppUL83 protein sequence NLVPMVATV 99 was attached to produce an antigenic lipopeptide bearing the SK 4 NLVPMVATV sequence. Strong up-regulation of the co-stimulatory molecule CD80 on human monocytes in fresh blood samples was observed using , and conditions to obtain either mono-or bis-palmitoylated cysteine derivatives were identified. 100 The molecule in Scheme 7 is a homologue of Pam 2 Cys with an extra methyl group in the glyceryl linker and was termed homoPam2Cys. It was isolated as a mixture of stereoisomers and was tested for NF-κB induction, and it showed the same activity as Pam 2 Cys itself. 20 The effect of the stereochemistry was investigated by preparing R and S enantiomers of Pam 2 Cys, homoPam2Cys, and analogues. 20 Based on the NY-ESO-1 protein sequence SLLMWITQC mentioned above and the HEK-Blue TLR2 reporter system, it was found that the R diastereomers have activity, whereas the S versions show a pronounced reduction in TLR2 activity. 20 Derivatives based on Pam 2 Cys bearing branched highly cationic or anionic N-termini have been designed (Scheme 8) in order to facilitate interaction with oppositely charged soluble protein antigens. 101, 102 The binding of lipopeptide to oppositely charged antigens leads to the formation of complexes at physiological pH. 101 The complexes elicit a CD8 + T cell immune response in mice, with concomitant pro-inflammatory cytokine production. Model antigens selected were ovalbumin (OVA) or hen egg lysozyme (HEL) with a net calculated charge of −11 or +8, respectively. The complexes were also shown to protect vaccinated mice against challenge with a live chimeric influenza virus engineered to contain the OVA CTL epitope SIINFEKL. The induced CD8 + T cell response correlated with the ability of lipopeptide to facilitate antigen uptake by dendritic cells (DCs). Oppositely charged lipopeptides were more effective in DC uptake and trafficking. Substantial antibody titers were produced by vaccination with complexes composed of oppositely charged lipopeptide and protein, whereas innoculation with similarly charged constituents resulted in measurable but lower antibody production. 101 Peptide R 4 Pam 2 Cys (Scheme 8) shows a strong ability to bind OVA, leading to the formation of large complexes (ca. 400 nm radius from DLS). 102 Excellent adjuvant activity was noted, from measurements of antibodies in mice and cytokine production. Antibody titers were higher for the lipopeptide/OVA mixture than those elicited by OVA in the presence of alum and were comparable to those elicited by OVA formulated with complete Freund's adjuvant (CFA). The activity of the D-Arg homologue of R 4 Pam 2 Cys was also examined and was shown to stimulate similar levels of antibody production, although CD8 + T cell responses (IFN-γ secretion) were reduced. 102 Covalent conjugates of Pam 3 CSK 4 with OVA 247−264 peptide epitope DEVSGLEQLESIINFEKL (or another peptide with this sequence and an A 5 K C-terminal extension) were prepared in a study of the TLR2 processing. 103 It was found that the uptake of the conjugates is TLR-independent, and inhibition of clathrin-or caveolin-dependent endocytosis greatly reduced uptake and antigen presentation of the Pam 3 CSK 4 conjugates. The lipopeptides induce a strong and specific T cell response due to the combined effects of enhanced antigen uptake, improved MHC class I antigen presentation, and dendritic cell maturation. 103 The Pam 2 Cys scaffold has been employed in the development of a range of immunogenic lipopeptides. In one example focused on the development of self-adjuvanting immunocontraceptive vaccines, Pam 2 Cys linkers (incorporated via lysine ε-amino units) were compared to Pam 3 Cys and control sequences just bearing lysine linkers, or other constructs with only C-terminal peptide sequences. 58 These bifunctional lipopeptides bear both the LHRH (luteinizing hormone releasing hormone) sequence EHWSYGLRPG and a T-helper epitope GALNNRFQIKGVELKS (from the L chain of influenza virus hemagglutinin). The lipopeptides induce antibodies against the "self" hormone LHRH in inoculated mice, without requiring additional adjuvant. The Pam 2 CSS lipopeptide attached laterally via a central K residue linking the two epitopes was highly effective, inducing high antibody titers, which were able to efficiently sterilize female mice when administered in saline by s.c. or intranasal routes. 58 The architecture with pendant Pam n CSS linked via the ε-amine group of lysine (Scheme 9) is also most effective in stimulating DC maturation via TLR2. 104 This was confirmed in a study using lipopeptides with this architecture bearing a Th sequence ALNNRFQIKGV-ELKS from influenza hemeagglutinin that elicits CD4 + T cells and a CTL peptide epitope TYQRTRALV, i.e., NP 147−155 , derived from the nucleoprotein of the influenza virus which is the dominant CD8+ T cell epitope recognized by BALB/c mice in all type A influenza strains. 104 In another example, bifunctional Pam 2 Cys-based derivatives were designed as a self-adjuvanting vaccine that induces neutralizing antibodies against heat-stable enterotoxin from enterotoxigenic E. coli. 105 Three constructs were synthesized with an N-terminal helper T cell epitope (GALNNRFQIKGV-ELKS, as above) and a C-terminal heat-stable enterotoxin (ST) tricylic peptide 106 NSSNYCCELCCNPACTGCY (C6−C11, C7−16, and C10−C18 disulfide links) with different linker groups to the ST peptide (Scheme 10). All three compounds generated specific anti-ST antibodies; however, the low titer antibodies (using a mouse model) induced by the oximecontaining derivative demonstrated better neutralizing activity when administered via the intranasal mucosal route. 105 As pointed out by Moyle and Toth, 47 the delivery of antigens mixed with, or conjugated to, lipopeptide adjuvants has the potential to produce vaccines that are immunogenic via mucosal routes, in particular nasal or oral.

The Jackson group showed that in fact this modular approach can be extended to produce immunogenic lipopeptides bearing LHRH or acid polymerase (PA) of influenza virus with sequence SSLENFRAYV or the ST enterotoxin mentioned above linked by oxime, thioether, or disulfide units to one of three T helper cell motifs. 107 The first was T h (OVA) derived from OVA, with sequence ISQAVHAAHAEINEAGR, the second was T h (FLU), . The authors note that using a thioether bond to conjugate the two components has the advantage of simpler chemistry and somewhat higher yield of product, whereas disulfide or oxime bond formation requires an additional synthesis step. Oxime bond formation, however, does have the advantage that the unwanted side products resulting from thiol groups present in cysteine residues (if present) may be avoided. The antibody and CD8 + T cell responses were found to depend on both the linker group and the Th epitope employed. 107 In a related study, the Th sequence LNNRFQIKGVELK derived from the light chain of influenza hemagglutinin was linked via the same linker with appended Pam 2 CSS (Scheme 9) to the CTL epitope, with sequence TYQRTRALV, mentioned above. 108 The Th domain was selected because it elicits CD4 + T cells that are cross-reactive with all H3 influenza viruses and the CTL (CD8 + ) epitope is common to all type A influenza strains. This lipopeptide can be delivered intranasally and is able to prime lung-resident memory CD8 + T cells for long-term pulmonary protection against influenza. 108 In another case, three Pam 2 Cys-based conjugates were prepared which contain a solubilizing spacer composed of either sequences of lysine residues or polyethylene glycol (PEG). 109 The model proteins HEL or bovine insulin were lipidated with the three types of lipid-spacer moieties, and the immunogenicity of the lipidated species was determined in mice by measuring antibody responses. The lipopeptides contain C-terminal cysteine residues which allow for formation of either a thioether or a disulfide bond with proteins (or peptides) derivatized with MCS [N-(ε-maleimidocaproyloxy acid succinimide ester] or SPDP [succinimidyl 3-(2-pyridyldithio)propionate], respectively. 109 The Pam 2 Cys scaffold has been used to attach both helper T cell Th and a target epitope that is either recognized by CD8 + T cells or B cells, using a diversity of Th and target epitopes. 110 These examples highlight research showing that the modular ("subunit") approach to the self-adjuvant vaccine development offers considerable scope to tune the immune response. A review is available that details methods to synthesize the lipopeptides designed by the Jackson group. 111 The Pam 3 CSS scaffold has been used along with the 16 Nterminal amino acids of the M. tuberculosis 19 kDa lipoprotein to give Pam 3 CSSNKSTTGSGETTTA which has been shown to be an agonist of RP105, a member of the TLR family that interacts with TLR2 and facilitates recognition of mature lipoproteins expressed by mycobacteria. 112 Mono-and dipalmityol analogues were also examined, as were peptide variants. These studies showed that, although the lipid moiety is required for macrophage activation, it is not a determinant of RP105 dependency. However, substitution of the T7 and T8 residues with nonpolar alanine residues led to reduced RP105 dependency. 112 Like Pam 3 CSK 4 , the M. tuberculosis 19 kDa lipoprotein (lipopeptide) stimulates T cell proliferation via TLR2 activity, producing IFN-γ in an accessory cell-dependent manner. Sieling et al. compared the activities of these two lipopeptides along with the Treponema pallidum lipoprotein TP47. 75 Treponema pallidum and Borrelia burgdorferi are the spirochetal pathogens responsible for syphilis and Lyme disease, respectively, and lipoproteins from the membranes of these organisms elicit a strong immunoinflammatory response, as potent activators of monocytes/macrophages. 113, 114 The OspA outer surface protein from B. burgderfori contains a post-translational amino-terminal Pam 3 Cys lipid moiety, and this component is essential in producing an antibody response. 115 The full OspA lipoprotein showed significantly better adjuvant activity than Pam 3 CSK 4 in a study of intranasal delivery. Synthetic lipohexapeptides corresponding to the N termini of the B. burgdorferi strain B31 OspA lipoprotein (CKQNVS) and the T. pallidum subspecies pallidum 47-kDa lipoprotein (CGSSHH) prepared as glyceryl cysteine derivatives (with a Pam 3 C motif) show similar activity, as does the unlipidated OspA hexapeptide. 114 The binding domains of the p19 M. tuberculosis 19 kDa lipoprotein to MHC class I binding motifs H-2D b and H-2L d have been identified. 116 Several samples from a series of PamCSS-based lipopeptides investigated elicit a CTL response, assayed as IFN-γ production from mouse splenocytes; in particular, lipopeptides which upregulated MHC-I molecules (with a H-2D b binding motif) showed this activity. 116 Self-adjuvant breast cancer vaccines based on Pam 3 CSK 4 were prepared using the HER2 (human epidermal growth factor receptor-2) epitope CH401, YQDTILWKDIFHKNNQ-LALT. 117 Other formulations included a lipopeptide based on this sequence in a single palmitoylated peptide with a diethylene glycol linker and mixtures of this with Pam 3 CSK 4 or the sphingolipid α-GalCer (a strong immunostimulant with antitumor properties) or Lipid A or a Pam-Th epitope QYIKANSKFIGITE (tetanus toxin-derived epitope). The conjugate self-adjuvanting vaccine produced a stronger antibody response (IgG titers from mice) than that of Pam 3 CSK 4 itself. The conjugate Pam 3 CKS 4 −CH401 conjugate self-assembles into globular aggregates, as revealed by TEM, whereas mixtures of PamCSK 4 and Pam-CH401 showed fibrillar co-assemblies, also observed for other mixtures containing Pam-CH401. The co-assembled structures also elicited a robust immune response. 117 The conformational and self-assembly properties of MALP-2 have been examined in detail. 118 MALP-2 is a macrophageactivating lipopeptide (hence the name) originally isolated from Mycoplasma fermentas and contains a 13-residue peptide GNNDESNISFKEK attached to a Pam2Cys linker (Scheme 2). 119 Whereas three lipid chain lipopeptides such as Pam 3 CSK 4 signal via TLR1 and TLR2 as discussed above, 20, 48, 58, 59, 75, 76 MALP-2, in common with other two-lipid-chain lipopeptides, 27 signals via TLR2/TLR6 heterodimers with CD36 as coreceptor. 76, 120, 121 Both MALP-2 and the constituent peptide adopt β-sheet conformations with different morphologies, with MALP-2 forming fibril rafts, whereas the peptide alone forms nanotape structures. 118 Pro-inflammatory responses in mouse lungs induced by MALP-2 and Pam 3 CSK 4 have been compared, considering the fact that the lungs have the highest expression of TLR2 receptors. The authors observed that the MALP-2dependent induction of Tnc (tenascin C, a glycoprotein involved in inflammation) may indicate the existence of TLR2/6-specific pathways. 76 The recognition of MALP-2 by TLR6 cooperatively with TLR2 was noted, together with the observation that TLR6 appears to discriminate between the Nterminal lipidated structures of MALP-2 and Pam 3 CSK 4 . 120 Indeed, by comparing MALP-2 with the variant of MALP-2 containing three rather than two palmitoyl chains, it was inferred that three-chain lipopeptides are recognized by TLR2 while twochain molecules (MALP-2 or lipoteichoic acid) require additional cooperation with TLR6. 121 In subsequent work, Muḧlradt's group compared the macrophage-stimulating activities of two lipopeptides isolated from Mycoplasma hyorhinis and several synthetic peptides including Pam 2 CSK 4 and Pam 3 CSK 4 with MALP-2. 122 The assays measured nitric oxide release assay with peritoneal macrophages from C3H/HeJ mice. The isolated peptides were analogues of MALP-2 containing (in some cases) different lipid chains (and in all cases different peptide sequences), i.e., S-[2,3-bisacyl(C16:0/C18:0)oxy-propyl]cysteinyl-GQTDNNSSQSQQPGSGTTNT and S-[2,3bisacyl (C16:0/C18:0)oxypropyl]cysteinyl-GQTN and an additional synthetic peptide S-[2,3-bis(palmitoyloxy)propyl]cysteinyl-GQTNT was examined. The GQTNT motif is from the determined variable lipopeptide C (VlpC) sequence. The lipopeptides showed pM activity, i.e., were as active as MALP-2, except for Pam 3 CSK 4 which showed activity above 100 pM. 122 A PEGylated version of MALP-2 has been shown to produce strong humoral and cellular immune responses against enterohemorragic E. coli after intranasal vaccination in a mixture with enterohemorragic E. coli antigens. 123 The MALP-2 derivative was effective as an adjuvant in this mucosal (nasal) vaccine study due to its improved solubility. MALP-2 has also been used in combination with gemcitabine (a chemotherapy medication) in a phase I/II clinical trial of an immunotherapy for patients with pancreatic carcinoma. 15 Lipopeptides have also been derived from the 44 kDa membrane-bound lipoprotein of Mycoplasma salivarium, including the N-terminal lipopeptide S-(2,3-bisacyloxypropyl)-cysteine-GDPKHPKSFTEWVA-, which was used as the basis to create the synthetic analogue S-(2,3-bispalmitoyloxypropyl)-cysteine-GDPKHPKSF. 124, 125 The latter is commercially available as FSL-1 (fibroblast-stimulating lipopeptide) 125 and, like other Mycoplasma derivatives such as MALP-2, is recognized by TLR2 and TLR6. 126 Variants of FSL-1 with a substitution of the C-terminal F for R or substitution of the palmitoyl lipid chains for stearoyl chains were also reported. 126 The fact that TLR2/6 agonists can reduce virus levels in the upper respiratory tract has motivated the recent investigation of the use of a Pam 2 Cys analogue in the treatment of COVID-19 caused by the SARS-Cov-2 virus. 127 Intranasal prophylactic administration of a compound INNA-051, a PEGylated Pam 2 Cys analogue (structure not disclosed but related to that shown in ref 109) , was shown to reduce SARS-CoV-2 transmission and to provide protection against COVID-19. Stimulation of TLR2 leads to activation of the innate immune response, reduced inflammation and tissue damage, and improved local epithelial barrier function. 127 However, investigating respiratory syncytial virus infections, Nguyen et al. concluded that modulation of infection using Pam 3 CSK 4 is independent of TLR activation. 128 This was based on the observation that two structurally related lipopeptides (PamCSK 4 and PHCSK, a negative control for Pam 3 CSK 4 lacking a methylene group in the thioether spacer) without TLR-signaling capacity did modulate RSV infection, whereas Pam 3 CSK 4 -based TLR1/2 agonists did not. 128 Studying COVID-19 disease severity and its relationship to TLR2 Conjugates of peptides with nucleotides have been prepared as TLR-dependent immunogens. In one example, the 2-alkoxy-8-hydroxyadenylpeptide conjugates shown in Scheme 11 were prepared. 130 The nucleotide component is believed to act as a TLR7 ligand. These conjugates bear the MHC class I epitope SIINFEKL from OVA. In comparison with a mixture of their individual components, these conjugates gave rise to enhanced antigen presentation in vitro but were found not to be able to induce DC activation.

Toth's group has developed so-called lipid core peptide (LCP) systems to deliver peptide epitopes at high density for a variety of applications, including the development of self-adjuvant vaccines. 47,131 Scheme 12 shows an example from a study that aimed to develop a peptide-subunit-based vaccine for hookworm infection. 132 The LCP in this example comprises three lipidic amino acid chains and a four-arm amine functionalized Bioconjugate Chemistry pubs.acs.org/bc Review dendrimer. The constructs prepared bear one of two peptide epitopes shown in Scheme 12. One is a B cell peptide epitope from the apical enzyme in the hemoglobin digestion cascade, the aspartic protease Na-APR-1 termed A291Y (peptide 1, Scheme 12), while chimeric peptide 2 contains the A 291 Y sequence flanked on either side by helix-promoting sequences from the yeast GCN4 protein. It was found that, while A291Y alone or the chimeric peptide with or without Freund's adjuvants induce negligible antibody responses, the LCP construct incorporating the chimeric peptide induces a strong IgG response in mice. The active chimeric peptide was designed to induce the native helical A291Y epitope conformation. 132 This group also developed lipopeptide-based conjugates in an effort to produce a Group A Streptococcus (GAS) vaccine. 133−136 The architectures of other constructs prepared by this group are shown in Scheme 13, and all contain the J14 peptide epitope KQAEKVKASREAKKQVEKALEQLEDKVK, in which the 14-residue peptide GAS M-protein C region (B cell epitope) is shown in bold, along with a universal helper T cell epitope KLIPNASLIENCTKAEL (P25). The LCP D in Scheme 13 contains two copies of J14 and P25. The vaccine of type A (Scheme 13) featuring a C-terminal palmitoyl lipid moiety, with P25 located at the N-terminus, and J14 attached to the side chain of a central lysine residue was capable of inducing the optimal antibody response after intranasal immunization in mice. 133, 136 This group had earlier developed a LPC bearing the 88/30 GAS sequence DNGKAIYERARERALQELGC and the J8 GAS peptide sequence QAEDKVKQSREAKKQVEKALK-QLEDKVQ (with bold core sequence related to that in J14) in the development of a self-adjuvant intranasal vaccine targeting the GAS M protein. 137, 138 The LCP vaccine formulation induced the elicitation of antigen-specific IgG production when administered with or without mucosal adjuvant cholera toxin B subunit, whereas cholera toxin B subunit was required for the induction of antigens. This group also developed human papilloma virus (HPV) vaccines based on an LCP bearing four copies of a 19 amino acid long sequence QAEPDRAHYNIV-TFCCKCD E 744−762 from the HPV16 E7 protein. 139 The sugar D-mannose was conjugated (N-terminally) to the LCP molecules to probe the effect of targeting dendritic cell mannose receptors on vaccine efficacy. The vaccines were able to clear or reduce the size of HPV-16 associated tumors in mice, the conjugates bearing mannose causing clearance or reduction in size of tumors to a greater extent than non-mannose-containing vaccines. 139 LCPs can be prepared by native chemical ligation (NCL), as exemplified in a study in which four copies of the thioester-modified 88/30 GAS peptide antigen (with C to P terminal residue substitution) were ligated using a C-terminal mercaptopropionic acid leucine inker onto an LCP scaffold featuring four cysteine residues. 140 In another report, multiple peptide epitopes including two 88/30 GAS epitopes, the J8 sequence, and PL1 VLTRRQSQDPKYVTQRIS, an S. pyogenes antigen, were attached to an LCP framework using NCL. 141, 142 Studies with mice revealed that high levels of systemic IgG antibodies were elicited against each of the incorporated peptides. 141 A triepitopic analogue with just one 88/30 GAS peptide attached was also prepared, and its immune response in terms of antibody titers and dendritic cell activation was assayed. 143, 144 6. LIPOPEPTIDE MICELLES Lipopeptide micelles have been developed as nanoparticles for self-adjuvant vaccines to treat Herpes simplex virus (HSV) infection. 145 The micelles (presumably spherical) self-assembled from PAs containing HSV envelope glycoprotein B (gB) and glycoprotein D (gD) fragments. The critical micelle concentrations were obtained from fluorescence probe assays, and micelles were sized using dynamic light scattering. The (IL-6, IL-8, IL-17, and IL-23) , macrophage inflammatory protein (MIP-2), and tissue necrosis factor TNF-α. 145 Lipopeptides that form cylindrical micelles have been shown to generate a strong IgG1 antibody response, and these peptide amphiphiles bearing a GAS B cell antigen act as self-adjuvanting vaccines for infections caused by these bacteria. 146 The peptide having a α-helical structure was conjugated to a dialkyl (dipalmitoyl, di-C 16 ) chain. The micelles were shown not to stimulate the pathogen-recognition receptor TLR2 in vitro, indicating that this is not the origin of the self-adjuvancy, in contrast to the Pam 3 CSK4 TLR agonist used for comparison (section 4); instead, this was ascribed to the nanoparticlemediated delivery of the peptide epitope. Thus, the lipopeptide system is non-immunogenic. 146 The Tirrell group also developed a micelle-forming lipopeptide containing a cytotoxic T cell epitope (SIINFEKL from OVA, discussed in section 4), linked to a di-palmitoyl (diC 16 ) chain. 147 This molecule forms rod-like micelles which have a self-adjuvant property and which were shown to act not via TLR2 but instead they likely enhance uptake by DCs by anchoring of the hydrophobic tails into cell membranes. In addition, the micelles induce an immune response by acting as antigen depots and providing a high density surface presenting the epitopes and reducing degradation. 147 The lipopeptides induce an antitumor antibody response in mice immunized with the micelles, in particular a reduction in tumor volume, improved survival rate, and cytotoxic T cell response. 147 

As the studies in this Review make clear, lipopeptides have considerable potential to play a valuable role in the development of vaccines, as antigens and/or adjuvants. There are pros and cons to the use of lipopeptides. Advantages include the ease of design and synthesis (and ability to perform large scale syntheses) of peptides along with the ability to tune conformation and self-assembly by lipidation, using established physicochemical principles. As also highlighted herein, there is scope to design active lipopeptides with a variety of architectures, i.e., configurations of lipids and peptides. In addition, lipopeptides offer the potential to tailor highly specific responses that can be achieved by precision sequence engineering and lipopeptide architecture. Other positives include the ability to prepare peptides at high purity, avoiding biological contamination and reduced allergic response. Disadvantages include the lack of the three-dimensional folded structure of a protein and hence restricted binding properties to human cells compared to actual antigens such as virus coat proteins. In addition, peptides containing native L-amino acid residues are susceptible to proteolysis and they can also have low immunogenicity. The limited stability of peptides in vivo can be overcome by a variety of strategies using non-natural amino acids, cyclization, etc. 13, 16 In addition, lipidation enhances stability in vivo, since it confers albumin-binding properties which enable longer circulation without degradation. 13,148−152 Lipopeptides have as yet found limited practical use in vaccines, although a considerable number of peptide and lipopeptide immunotherapies are in development. 16−19 To be developed for practical uses, immune responses in humans should be studied as part of later stage clinical trials. Most research discussed above has focused on in vitro studies and those using rodent models of immune response. However, there can be significant differences in the PRRs presented in human and animal cells. The use of humanized mice or nonhuman primates as better models has thus been proposed. Also, the delivery route for many animal studies differs from the subcutaneous or intramuscular delivery used for practical vaccine delivery. 21 It has also been pointed out that the dose in many animal studies may differ substantially from that applicable to human use. A further limitation is the use of cell culture methods for in vitro studies, since these do not detect inflammatory responses generated by noncirculating tissue cells. 21 The choice of antigen used together with a particular adjuvant will affect the response of DCs, for example whether a whole protein or fragment antigen such as peptide sequences discussed in this Review is selected. As highlighted in this Review, the presentation of the antigen at the surface of a self-assembled structure such as those formed by lipopeptides can also influence the immune response. In many of the cases discussed in this Review, the self-assembly and conformation of the lipopeptides has not been examined, and this is a subject worth further examination, since self-assembly may have a significant effect on bioactivity.

For successful application, lipopeptide-based adjuvants and antigens will clearly need to have an excellent safety profile. Fortunately, current adjuvanted nonlive vaccines (i.e., the type corresponding to lipopeptides) are considered to generally be insufficiently immunogenic to trigger autoimmunity. This has been well established for the widely used oil-in-water adjuvants MF59 and AS03, 21 and as discussed herein, some lipopeptide systems have been developed to the stage of clinical trials and do not seem to cause serious adverse reactions.

As mentioned in section 4, TLRs including those that are agonized by lipopeptides such as the Pam n Cys type are important in COVID-19 pathogenesis, 153, 154 and this has led to a Pam 2 Cys-based intranasal prophylactic INNA-051 which is under commercial development. 127 This area is likely to be the focus of further intense research activity as the current pandemic continues to have major global effects. As outlined in this Review, lipopeptides also hold great promise in the development of vaccine treatments for many other serious infectious diseases and cancer, and thus have the potential to deliver valuable novel therapeutics based on a diversity of modes of action. 

SARS-CoV-2 vaccines in development

Current status of COVID-19 (pre)clinical vaccine development

COVID-19 vaccines: where we stand and challenges ahead

Vaccine formulations in clinical development for the prevention of severe acute respiratory syndrome coronavirus 2 infection

Immunogenicity of clinically relevant SARS-CoV-2 vaccines in nonhuman primates and humans

COVID-19 vaccines: rapid development, implications, challenges and future prospects

Peptide based amphiphiles

Molecular self-assembly and applications of designer peptide amphiphiles

Peptide selfassembly for crafting functional biological materials

Self-Assembly of amphiphilic peptides

Selfassembling amphiphilic peptides

Lipopeptides: from self-assembly to bioactivity

Introduction to Peptide Science

Clinical safety of HIV lipopeptides used as vaccines in healthy volunteers and HIVinfected adults

Intratumoural injection of the Toll-like receptor-2/6 agonist 'macrophage-activating lipopeptide-2′ in patients with pancreatic carcinoma: a phase I/II trial

More than one reason to rethink the use of peptides in vaccine design

Synthetic peptide vaccines

Peptide-based synthetic vaccines

Peptide-based vaccines: Current progress and future challenges

Synthesis and evaluation of novel TLR2 agonists as potential adjuvants for cancer vaccines

Vaccine adjuvants: Putting innate immunity to work

Applications of Immunomodulatory Immune Synergies to Adjuvant Discovery and Vaccine Development

Enhancing immunity with nanomedicine: Employing nanoparticles to harness the immune system

Toll-like receptor signalling

Microbial recognition by Tolllike receptors

Newly described pattern recognition receptors team up against intracellular pathogens

Modern subunit vaccines: development, components, and research opportunities

Contributions of humoral and cellular immunity to vaccine-induced protection in humans

Tailoring inorganic nanoadjuvants towards next-generation vaccines

Adjuvants -A classification and review of their modes of action

Advances in vaccine adjuvants

Adjuvants -a balance between toxicity and adjuvanticity

Vaccine adjuvants revisited

Emerging adjuvants for cancer immunotherapy

A genital tract peptide epitope vaccine targeting TLR-2 efficiently induces local and systemic CD8 + T cells and protects against herpes simplex virus type 2 challenge

Long-term specific immune responses induced in humans by a human immunodeficiency virus type 1 lipopeptide-vaccine: Characterization of CD8 + -T-cell epitopes recognized

Characterization of a multi-lipopeptides mixture used as an HIV-1 vaccine candidate

Multiepitopic B-and T-cell responses induced in humans by a human immunodeficiency virus type 1 lipopeptide vaccine

Sustained control of viremia following therapeutic immunization in chronically HIV-1-infected individuals

Long-term multiepitopic cytotoxic-T-lymphocyte responses induced in chimpanzees by combinations of Plasmodium falciparum liver-stage peptides and lipopeptides

Lipopeptide epitopes extended by an N εpalmitoyl-lysine moiety increase uptake and maturation of dendritic cells through a Toll-like receptor-2 pathway and trigger a Th1-dependent protective immunity

Simultaneous lipidation of a characterized peptide mixture by chemoselective Ligation

Chemoselective acylation of fully deprotected hydrazino acetyl peptides. Application to the synthesis of lipopeptides

Single-chain lipopeptide vaccines for the induction of virus-specific cytotoxic T cell responses in randomly selected populations

Chemoselective ligation and antigen vectorization

Th-cytotoxic T-lymphocyte chimeric epitopes extended by N-epsilon-palmitoyl lysines induce herpes simplex virus type 1-specific effector CD8(+) Tc-1 responses and protect against ocular infection

Self-adjuvanting lipopeptide vaccines

Cutting edge: Role of Toll-like receptor 1 in mediating immune response to microbial lipoproteins

Two lipoproteins extracted from Escherichia coli K-12 LCD25 lipopolysaccharide are the major components responsible for Toll-like receptor 2-mediated signaling

Cutting edge: Inflammatory signaling by Borrelia burgdorferi lipoproteins is mediated by Toll-like receptor 2

The Lip lipoprotein from Neisseria gonorrhoeae stimulates cytokine release and NF-sB activation in epithelial cells in a Toll-like receptor 2-dependent manner

) the dual role of lipids of the lipoproteins in Trumenba, a self-adjuvanting vaccine against meningococcal meningitis B disease

Separation and structural analysis of lipoprotein in a lipopolysaccharide preparation from Porphyromonas gingivalis

Characterization of N-terminal structure of TLR2-activating lipoprotein in Staphylococcus aureus

Lipoproteins of grampositive bacteria: key players in the immune response and virulence. Microbiol

TLR ligand-peptide conjugate vaccines: Toward clinical application

agonists and their structure-activity relationships

Highly immunogenic and totally synthetic lipopeptides as self-adjuvanting immunocontraceptive vaccines

Immunoprofiling toll-like receptor ligands. Comparison of immunostimulatory and proinflammatory profiles in ex vivo human blood models

Cutting edge: Activation of Toll-like receptor 2 induces a Th2 immune response and promotes experimental asthma

Promotes Th2/Th17 responses via TLR4 and TLR7/8 by abrogating the type I IFN amplification loop

TLR2-activated human Langerhans cells promote Th17 polarization via IL-1 beta, TGF-beta and IL-23

Toll-like receptor 2 signaling in CD4 + T lymphocytes promotes T helper 17 responses and regulates the pathogenesis of autoimmune disease

Cutting edge: TLR2 directly triggers Th1 effector functions

Crystal structure of the TLR1-TLR2 heterodimer induced by binding of a tri-acylated lipopeptide

Recognition of lipopeptide patterns by Toll-like receptor 2-Toll-like receptor 6 heterodimer

Covalent lipoprotein from outer membrane of Escherichia coli

Covalent binding of lipid to protein -diglyceride and amide-linked fatty-acid at N-terminal end of murein-lipoprotein of Escherichia coli outer membrane

Induction of lymphocyte-proliferation and membrane changes by lipopeptide derivatives of lipoprotein from outer membrane of Escherichia coli

B-Lymphocyte mitogenicity in vitro of a synthetic lipopeptide fragment derived from bacterial lipoprotein. Hoppe-Seylers

Synthetic lipopeptide analogs of bacterial lipoprotein are potent polyclonal activators for murine B lymphocytes

A synthetic analogue of Escherichia coli lipoprotein, tripalmitoyl pentapeptide, constitutes a potent immune adjuvant

Lipopeptide derivatives of bacterial lipoprotein constitute potent immune adjuvants combined with or covalently coupled to antigen or hapten

Solidphase peptide-synthesis of lipopeptide vaccines eliciting epitopespecific B-helper, T-helper and T-killer cell response

Toll-like receptor 2 ligands as adjuvants for human Th1 responses

Effects of the TLR2 agonists MALP-2 and Pam 3 Cys in isolated mouse lungs

Toll-like receptor agonist lipopeptides self-assemble into distinct nanostructures

Self-assembled micellar structures of lipopeptides with variable number of attached lipid chains revealed by atomistic molecular dynamics simulations

Lipopeptide structure determines TLR2 dependent cell activation level

TLR1-and TLR6-independent recognition of bacterial lipopeptides

Toll-like receptor 6-independent signaling by diacylated lipopeptides

Immunostimulants and Toll-like receptor ligands obtained by screening combinatorial lipopeptide collections

Synthetic lipopeptide adjuvants and Toll-like receptor 2 -structure-activity relationships

Preferentially the Rstereoisomer of the mycoplasmal lipopeptide macrophage-activating lipopeptide-2 activates immune cells through a Toll-like receptor 2-and MyD88-dependent signaling pathway

Synthesis of N-alpha-Fmoc protected derivatives of S-(2,3-dihydroxypropyl)-cysteine and their application in peptide-synthesis

Lipopeptides containing 2-(palmitoylamino)-6,7-bis(palmitoyloxy)heptanoic acid -synthesis, stereospecific stimulation of B-lymphocytes and macrophages, and adjuvanticity in vivo and in vitro

Structure-activity relationships in Toll-like receptor-2 agonistic diacylthioglycerol lipopeptides

) structureactivity relationships in Toll-like receptor 2-agonists leading to simplified monoacyl lipopeptides

Lipolanthionine peptides act as inhibitors of TLR2-mediated IL-8 secretion. Synthesis and structure-activity relationships

Physicochemical and biological analysis of synthetic bacterial lipopeptides -Validity of the concept of endotoxic conformation

Chirality of TLR-2 ligand Pam 3 CysSK 4 in fully synthetic peptide conjugates critically influences the induction of specific CD8 + T-cells

N-Tetradecylcarbamyl lipopeptides as novel agonists for Toll-like receptor 2

Comparison of adjuvant formulations for cytotoxic T cell induction using synthetic peptides

Lipopeptide-polyoxyethylene conjugates as mitogens and adjuvants

Evaluation of a lipopeptide immunogen as a therapeutic in HIV type 1-seropositive individuals

Direct peptide lipidation through thiol-ene coupling enables rapid synthesis and evaluation of self-adjuvanting vaccine candidates

Direct peptide lipidation through thiol-ene coupling enables rapid synthesis and evaluation of self-adjuvanting vaccine candidates (erratum)

Sequence flexibility of the immunodominant HLA A*0201 restricted ppUL83 CD8 T-Cell epitope of human cytomegalovirus

Lipidation of cysteine or cysteine-containing peptides using the thiol-ene reaction (CLipPA)

Soluble Proteins Induce Strong CD8 + T cell and antibody responses through electrostatic association with simple cationic or anionic lipopeptides that target TLR2

Structure-function relationships of proteinlipopeptide complexes and influence on immunogenicity

Distinct uptake mechanisms but similar intracellular processing of two different Toll-like receptor ligand-peptide conjugates in dendritic cells

Lipid-containing mimetics of natural triggers of innate immunity as CTL-inducing influenza vaccines

A totally synthetic lipopeptide-based self-adjuvanting vaccine induces neutralizing antibodies against heat-stable enterotoxin from enterotoxigenic Escherichia coli

Heat-Stable enterotoxin of Enterotoxigenic Escherichia coli as a vaccine target

A modular approach to assembly of totally synthetic selfadjuvanting lipopeptide-based vaccines allows conformational epitope building

Intranasal lipopeptide primes lung-resident memory CD8 + T cells for long-term pulmonary protection against influenza

Lipidation of intact proteins produces highly immunogenic vaccine candidates

A totally synthetic vaccine of generic structure that targets Toll-like receptor 2 on dendritic cells and promotes antibody or cytotoxic T cell responses

Peptide-based drug design

The N-terminal peptide moiety of the Mycobacterium tuberculosis 19 kDa lipoprotein harbors RP105-agonistic properties

Lipoproteins of Borrelia burgdorferi and Treponema pallidum activate cachectin tumornecrosis-factor synthesis -analysis using a cat reporter construct

Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides activate monocytic cells via a CD14-dependent pathway distinct from that used by lipopolysaccharide

OspA lipoprotein of Borrelia burgdorferi is a mucosal immunogen and adjuvant

Evaluation of T-cell responses to peptides and lipopeptides with MHC class I binding motifs derived from the amino acid sequence of the19-kDa lipoprotein of Mycobacterium tuberculosis

Immunological evaluation of coassembling a lipidated peptide antigen and lipophilic adjuvants: selfadjuvanting anti-breast-cancer vaccine candidates

Self-assembly of the Toll-like receptor agonist macrophage-activating lipopeptide MALP-2 and of its constituent peptide

Isolation, structure elucidation, and synthesis of a macrophage stimulatory lipopeptide from Mycoplasma fermentans acting at picomolar concentration

Discrimination of bacterial lipoproteins by Toll-like receptor 6

Differential recognition of structural details of bacterial lipopeptides by Toll-like receptors

Structure and specific activity of macrophage-stimulating lipopeptides from Mycoplasma hyorhinis

Efficient immune responses against Intimin and EspB of enterohaernorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant

The N-terminal lipopeptide of a 44-kDa membrane-bound lipoprotein of Mycoplasma salivarium is responsible for the expression of intercellular adhesion molecule-1 on the cell surface of normal human gingival fibroblasts

Signaling pathways induced by lipoproteins derived from Mycoplasma salivarium and a synthetic lipopeptide

Relationship between structures and biological activities of mycoplasmal diacylated lipopeptides and their recognition by Toll-like receptors 2 and 6

Prophylactic intranasal administration of a TLR2/6 agonist reduces upper respiratory tract viral shedding in a SARS-CoV-2 challenge ferret model

The synthetic bacterial lipopeptide Pam3CSK4 modulates respiratory syncytial virus infection independent of tlr activation

2021) TLR2 senses the SARS-CoV-2 envelope protein to produce inflammatory cytokines

Synthesis of 2-alkoxy-8-hydroxyadenylpeptides: Towards synthetic epitope-based vaccines

Lipid core peptide system for gene, drug, and vaccine delivery

Peptide-based subunit vaccine against hookworm infection

Structure-activity relationship of a series of synthetic lipopeptide selfadjuvanting group A streptococcal vaccine candidates

Design of three-component vaccines against group a streptococcal infections: importance of spatial arrangement of vaccine components

Structure-activity relationship of lipopeptide Group A streptococcus (GAS) vaccine candidates on Tolllike receptor 2

Immunological evaluation of Lipopeptide Group A streptococcus (GAS) vaccine: structure-activity relationship

Enhancing the immunogenicity and modulating the fine epitope recognition of antisera to a helical group A streptococcal peptide vaccine candidate from the M protein using lipid-core peptide technology

Intranasal administration is an effective mucosal vaccine delivery route for self-adjuvanting lipid core peptides targeting the group A streptococcal M protein

Toward the development of prophylactic and therapeutic human papillomavirus type-16 lipopeptide vaccines

A technique for the synthesis of highly-pure, monoepitopic, multi-valent lipid core peptide vaccines

Method for the synthesis of highly pure vaccines using the lipid core peptide system

Method for the synthesis of multiepitopic Streptococcus pyogenes lipopeptide vaccines using native chemical ligation

Synthesis of a highly pure lipid core peptide based selfadjuvanting triepitopic group A Streptococcal vaccine, and subsequent immunological evaluation

A novel synthetic adjuvant enhances dendritic cell function

Selfassembled or mixed peptide amphiphile micelles from Herpes simplex virus glycoproteins as potential immunomodulatory treatment

Peptide amphiphile micelles self-adjuvant group a streptococcal vaccination

Self-assembled peptide amphiphile micelles containing a cytotoxic T-Cell epitope promote a protective immune response in vivo

Potent derivatives of glucagon-like peptide-1 with pharmacokinetic properties suitable for once daily administration

Long-acting lipidated analogue of human pancreatic polypeptide is slowly released into circulation

Peptide hormones and lipopeptides: from self-assembly to therapeutic applications

The effect of lipidation on the self-Assembly of the gut-derived peptide hormone PYY 3−36

Targeting human TLRs to combat COVID-19: A solution?

Role of Toll-like receptors in the pathogenesis of COVID-19