key: cord-0913839-jezr42eh
authors: Alexiev, Ivailo; Ivanov, Ivan; Philipova, Ivva; Korsun, Nelly; Stoikov, Ivan; Dimitrova, Reneta; Grigorova, Lyubomira; Gancheva, Anna; Trifonova, Ivelina; Dobrinov, Veselin; Grigorova, Iliana; Savov, Alexey; Asenova, Boryana; Ciccozzi, Massimo; Kantardjiev, Todor
title: Postvaccination SARS‐CoV‐2 Alpha (B.1.1.7) lineage infection among healthcare workers on the background of IgG antibodies
date: 2021-10-20
journal: J Med Virol
DOI: 10.1002/jmv.27394
sha: b122dc3fad0085d0167d85a7f7baf49bded4c8b4
doc_id: 913839
cord_uid: jezr42eh

Like most vaccines, the effectiveness of COVID-19 vaccines developed so far is not 100% and a small percentage of fully vaccinated individuals still develop symptomatic or asymptomatic SARS-CoV-2 infections. 1 In addition, the emerging of novel mutations is likely to help the virus evade vaccines especially in regions with low vaccination coverage. 2 This article is protected by copyright. All rights reserved.

Pangolin COVID-19 Lineage Assigner Tool v3. 1.7 . was used to define the variant classification. 3 The dataset for the phylogenetic analysis contained sequences from both samples under investigation together with 112 other randomly selected SARS-CoV-2 sequences isolated in Bulgaria and the reference sequence employed by GISAID (EPI_ISL_402124).

Sequence alignments were performed using MAFFT version 7. 4 All Bulgarian SARS-CoV-2 sequences were deposited in GISAID databases, the dataset and sequence accession IDs are available upon request.

The potential phylogenetic relationship of the SARS-CoV-2 S gene clades was evaluated by approximate maximum-likelihood phylogenies using the GTR nucleotide substitution model in FastTree v2. 1.10. 5 Nonsynonymous mutations were defined by the Internet available Genome Detective Coronavirus Typing Tool. 6 Case 1 is a 43-year-old woman, working in the COVID-19 ward during the second pandemic wave in the country, and Case 2 is a 44-year-old woman, who works in the Endocrinology and metabolic diseases ward, both with no evidence of chronic illness, see Table 1 . 

The authors declare no conflict of interest. 

Postvaccination COVID-19 among Healthcare Workers

Escape from neutralizing antibodies by SARS-CoV-2 spike protein variants

A dynamic nomenclature proposal for SARS-CoV-2 lineages to assist genomic epidemiology

MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization

FastTree 2-approximately maximumlikelihood trees for large alignments

Genome Detective Coronavirus Typing Tool for rapid identification and characterization of novel coronavirus genomes

Safety and efficacy of the BNT162b2 mRNA Covid-19 vaccine

Risk related to the spread of new SARS-CoV-2 variants of concern in the EU/EEA -first update