key: cord-0951735-87vofkx5 authors: Cao, Xing; Wang, Yafei; Li, Panyun; Huang, Wei; Lu, Xiaojuan; Lu, Hongda title: HBV Reactivation During the Treatment of Non-Hodgkin Lymphoma and Management Strategies date: 2021-07-01 journal: Front Oncol DOI: 10.3389/fonc.2021.685706 sha: 7e4c23fa98e022a44aa211c743defe355c8163b6 doc_id: 951735 cord_uid: 87vofkx5 Hepatitis B virus reactivation (HBV-R), which can lead to HBV-related morbidity and mortality, is a common and well-known complication that occurs during the treatment of non-Hodgkin lymphoma (NHL) patients with current or past exposure to HBV infection. HBV-R is thought to be closely associated with chemotherapeutic or immunosuppressive therapies. However, immunosuppressive agents such as anti-CD20 antibodies (e.g., rituximab and ofatumumab), glucocorticoids, and hematopoietic stem cell transplantation (HSCT) administered to NHL patients during treatment can cause deep immunodepression and place them at high risk of HBV-R. In this review, we explore the current evidence, the guidelines of several national and international organizations, and the recommendations of expert panels relating to the definition, risk factors, screening and monitoring strategies, whether to use prophylaxis or pre-emptive therapy, and the optimal antiviral agent and duration of antiviral therapy for HBV-R. Hepatitis B virus (HBV) is a double-stranded DNA virus belonging to the family Hepadnaviridae (1) . HBV infection has reached epidemic proportions globally. It is estimated that over one-third of the world's population has been infected with HBV, with approximately 248 million of them suffering from chronic infection (2, 3) . Compared with uninfected individuals, those infected with HBV have a 2-3-fold greater risk of developing non-Hodgkin's lymphoma (NHL), particularly diffuse large B-cell lymphoma (DLBCL), which represents the major NHL subtype (4) (5) (6) (7) (8) (9) . Although the mechanism underlying this phenomenon remains unclear, it is likely to be due to the hepatotropic and lymphotropic nature of HBV, which can assure HBV replication in lymphoid tissue (10) (11) (12) . Interestingly, it has been reported that HBV infection is uncorrelated with Hodgkin's lymphoma (HL) (13, 14) . HBV reactivation (HBV-R) is defined as resolved/occult HBV that becomes active again, leading to adverse consequences. Occult HBV infection is characterized by the presence of replication-competent HBV DNA (i.e., covalently closed circular DNA [cccDNA] comprising the episomal HBV genome) in the liver and/or blood of hepatitis B surface antigen (HBsAg)-negative individuals as determined by currently available assays (15) . Over recent years, a close link has been established between HBV-R and cytotoxic chemotherapeutic drugs, such as anthracyclines, cyclophosphamide, vincristine, and prednisone, as well as anti-CD20 monoclonal antibody therapy (e.g., rituximab) (16) (17) (18) (19) (20) (21) . Nevertheless, an R-CHOP regimen comprising rituximab, anthracyclines, cyclophosphamide, vincristine, and prednisone is now widely used as the first-line treatment for NHL, highlighting the need to be vigilant for HBV-R in concerned patients. The rate of HBV-R in HBsAg(+) patients who undergo rituximab-containing therapy is reported to range from 33 to 65% and between 6 and 24% in those negative for HBsAg and positive for antihepatitis B core antibody (HBcAb), respectively (22) . The clinical manifestations of HBV-R can vary from asymptomatic hepatitis to lethal liver failure (23) . Additionally, patients with HBV-R may postpone scheduled chemotherapy or present with abnormal liver function, leading to adverse effects on treatment outcome for the primary disease (24, 25) . There are no standard screening, monitoring, or management strategies for HBV-R, and recommendations for the clinical management of HBV-R for patients treated with R-CHOP differ among institutions. Here, we review the recent literature relating to HBV-R, as the appropriate and timely identification of HBV-R, as well as suitable strategies for its management, remain crucial for improving the quality of life of NHL patients. No uniform criteria for the definition of HBV-R currently exist and different guidelines have heterogeneous definitions for HBV-R diagnosis and management. The American Association for the Study of Liver Diseases (AASLD), the Asian Pacific Association for the Study of the Liver (APASL), and the American Gastroenterological Association (AGA) give clear definitions of HBV-R (26) (27) (28) , whereas the European Association for the Study of the Liver (EASL) does not (29) . Table 1 summarizes the definitions of HBV-R based on the different guidelines. Additionally, the AASLD also provides explicit concepts for HBV-associated hepatitis, namely, acute serum alanine aminotransferase (ALT) levels ≥3-fold higher than that at baseline and an absolute value >100 U/L (26) . Interestingly, it has been reported that monitoring ALT levels can lead to the earlier detection of HBV-R because ALT levels increase 2-3 weeks before a rise in HBV DNA levels is detected (31) . Collectively, HBV-R can be identified in a timely manner by combining ALT levels with HBV DNA and HBsAg. In HBsAg(+) patients, a dramatic rise in HBV DNA concentrations (usually 100-fold or more) are indicative of HBV-R, while in patients with resolved HBV infection (HBsAg[−] and HBcAb[+]), HBV-R usually means the reappearance of HBsAg or an increase in serum HBV DNA concentrations with or without HBsAg seroconversion and ALT exacerbation. Notably, HBV-R can be induced by coinfection with HBV and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (32) (33) (34) . Other coinfections, such as that with HCV or HDV, can also increase the likelihood of HBV-R (35) . The crucial risk factors for HBV-R can be classified into three types, namely, host-related, virus-related, and medication-related (35) . Medication-related factors are usually associated with underlying disease that requires immunosuppressive therapy, such as chemotherapy, solid organ or bone marrow transplantation, rheumatological conditions, dermatological conditions, or inflammatory bowel disease) (36) . As mentioned above, the drugs that are currently used to treat NHL (anthracyclines, cyclophosphamide, vincristine, prednisone, and anti-CD20 monoclonal antibody) are usua lly immunosuppressive, and constitute the focus of this review. One of the following: 1) a ≥100-fold elevation in the HBV DNA load compared with the baseline level; 2) ≥1,000 IU/ml of HBV DNA with an undetectable baseline level; 3) ≥10,000 IU/ml HBV DNA if the baseline level is not available. One of the following: 1) HBV DNA is detectable; 2) reappearance of HBsAg The Asian Pacific Association for the Study of the Liver. (APASL, 2016) (27) One of the following: 1) a ≥100-fold increase in the HBV DNA load from baseline levels; 2) the reappearance of HBV DNA to a level of 100 IU/ml if the baseline level is undetectable; 3) ≥20,000 IU/ml HBV DNA if the baseline level is not available. One of the following: 1) reverse HBsAg seroconversion (reappearance of HBsAg); 2) the appearance of HBV DNA in serum if HBsAg is negative American Gastroenterological Association. (AGA, 2015) (30) One of the following: 1) de novo detectable HBV DNA if baseline level is undetectable; 2) a ≥10-fold increase in HBV DNA levels if the baseline DNA level is detectable. Males are more prone to undergoing HBV-R than females. Yeo et al. reported that in 600 HBsAg(+) cancer patients exposed to chemotherapy, the HBV-R ratio was almost three-fold higher in men than women (37, 38) . Additionally, people that are more than 50 years old, HBeAg(+), and with underlying disease that requires immunosuppressive therapy (e.g. lymphomas) are at greater risk of developing HBV-R (37). The virus-related, high-risk factors for HBV-R identified to date include detectable HBV DNA, HBsAg and HBcAb positivity, mutations in HBsAg, and HBV genotype (23, 39) . Among these, detectable HBV DNA was reported to be the most important predictive factor for HBV-R. In one study, 37 Among the HBsAg mutations identified, 61.5% were found to reside in a major hydrophilic region, while some were known immune escape-associated mutations, such as the sD144E mutation that disrupts humoral response-mediated HBsAg recognition. The remaining mutations were found to reside in class-I/II-restricted Tcell epitopes, suggesting that they were important for HBV escape from T-cell-mediated responses (40) . HBsAg(+) patients have an eight-fold higher likelihood of HBV-R when compared with patients with resolved infection (HBsAg[−] and HBcAb[+]) (41) . Notably, HBV genotype A is rarely involved in HBV-R, while other genotypes are reported (35, 42) . Coinfection with other viruses, such as HCV, HDV, HIV, or SARS-CoV-2, also puts patients at higher risk of HBV-R, as mentioned before (26, 35) . Numerous studies have indicated that immunosuppressive and chemotherapeutic medications represent the major risk for HBV-R (43) (44) (45) . The greatest and most reported risk for HBV-R is associated with B-cell-depleting therapy, such as that with the anti-CD20 antibody, rituximab (35) . The AGA and the AASLD have stratified the HBV-R-related risk of individual medications or therapies (26, 30) . Additionally, the risk of HBV-R has been graded through different immunosuppressive treatments and HBV infection status, as follows ( Table 2) : (1) Very high risk, greater than 20% chance of reactivation, and is associated with anti-CD20 antibody therapy (rituximab or ofatumumab) and hematopoietic stem cell transplantation (HSCT); (2) high risk, between 10 and 20% chance of reactivation, and is mainly related to high-dose glucocorticoid and anthracycline treatment; (3) moderate risk, between 1 and 10% chance of reactivation; (4) low risk, less than 1% chance of reactivation (30, 35, (45) (46) (47) . Importantly, the immunosuppressive therapies included in the very-high-and high-risk groups are those usually administered to NHL patients, rendering them prone to HBV-R and incidental adverse events. Rituximab, approved in 1997, is used for the treatment of NHL and chronic lymphocytic leukemia. Numerous cases of HBsAg(+) lymphoma patients treated with rituximab-containing therapy developing HBV-R have been documented since 1999 (48, 49) . Rituximab and ofatumumab are humanized anti-CD20 monoclonal antibodies targeting CD20, a cell-surface marker on B lymphocytes, resulting in B-cell depletion and the subsequent impairment of Bcell antigen-presenting function, with the consequent reduction of specific anti-HBV CD4-positive T-cell activation and proliferation (50) . Rituximab affects cell signaling by directly inducing the apoptosis of malignant B cells and activating complementdependent cytotoxicity. These effects result in the rapid death of rituximab-targeted cells and the activation of natural killer (NK) cells, which then produce interferon-gamma (IFNg), and thereby induce antibody-dependent cellular cytotoxicity when they interact with rituximab-coated target cells (51) . Ofatumumab functions through a similar mechanism. These types of drugs are commonly associated with serious HBV-R- related events and can increase the risk of hepatocyte dysfunction and mortality if HBV-R is not quickly identified and cleared (35, 52) . Few retrospective data are available for the incidence of HBV-R in HBsAg(+) patients treated with rituximab-containing therapy without any antiviral prophylaxis. The HBV-R rate in NHL patients has been reported to vary between 18.2 and 80%. HBV-R occurs from after a few weeks to up to 55 months after rituximab administration, indicating that rituximab represents a very high-risk factor for HBV-R (48, (53) (54) (55) (56) (57) . Between 1997 and 2009, the US Food and Drug Administration (FDA) MedWatch Database reported 118 cases of HBV-R. Among these patients, those administered rituximab-containing therapy had a prominently increased risk of HBV-R compared with those given non-rituximab-containing therapy (OR 5.73, 95% CI 2.01-16.33, P = 0.0009) without heterogeneity (19) . Consequently, the FDA alerted healthcare professionals that rituximab and ofatumumab were associated with a high risk of HBV-R and added "boxed warnings" (the strongest warnings) to the product labels in September 2013 (58) . In the Emerging Trends Conference sponsored by the AASLD held in 2015, entitled "Reactivation of Hepatitis B," HBV-R was reported to be a possible underestimated clinical challenge related to ofatumumab or rituximab treatment. Furthermore, it was suggested that all patients undergoing ofatumumab-or rituximab-containing therapies should be screened for HBV-R, and that HBsAg(+) or HBcAb(+) patients should start prophylactic antiviral therapy to prevent HBV-R (59). Collectively, these data indicate that rituximab and other Bcell-depleting therapies pose the greatest risk for HBV-R (35, 46, 60) , which warrants vigilance by the medical community. Corticosteroids were first reported to be associated with HBV-R by Sagnelli et al. in 1980 , which was subsequently widely confirmed (38, 61) . The risk for HBV-R with corticosteroid use is deemed to be dose-and time-dependent, with studies having indicated that a high dose (>20 mg/day) of chronic prednisone therapy for longer than 4 weeks is associated with a high risk of HBV-R (28, 62). Cheng et al. conducted a randomized study on 50 HBsAg(+) lymphoma patients and compared the HBV-R rate in patients undergoing an identical chemotherapeutic regimen receiving or not corticosteroid treatment. The authors reported that the cumulative incidence of HBV-R at 9 months was significantly higher in the corticosteroid treatment group (38 vs. 73%, P = 0.03) (63) . In a recent 6-year prospective cohort study, HBV-R was reported to occur a median of 10 months (range, 4-32) after steroid administration (53) . Corticosteroids enhance HBV replication mainly through two mechanisms. First, they suppress cell-mediated immunity via the inhibition of interleukins, which then prevents T and B cell proliferation (43) . Second, corticosteroids stimulate the glucocorticoid-responsive element present in the HBV genome, thereby exerting a direct suppressive effect on T-cell-mediated immunity (23, 64) . Corticosteroids were also reported to be able to increase HBsAg secretion via inducing autophagy, i.e., inhibiting autophagy using 3-MA, an autophagy inhibitor, decreased HBV replication and HBsAg secretion (65); however, this possibility requires further investigation. These observations suggest that the reported HBV-R in patients coinfected with SARS-CoV-2 and HBV might be associated with corticosteroid use during the treatment of COVID-19. The R-CHOP regimen, which contains prednisone, is still conventionally administered to patients with NHL as first-line standard therapy. Hence, the risk for HBV-R should be taken into consideration when treating NHL patients with a corticosteroid-containing regimen. Anthracyclines, such as doxorubicin and epirubicin, have also been linked to a high risk of HBV-R (35, 66) . This class of chemotherapeutic drugs is applied to treat a variety of solid and hematological cancers, including lymphoma, bladder cancer, softtissue sarcoma, leukemia, breast cancer, and multiple myeloma. Doxorubicin exerts its HBV-R-related effects by increasing the expression of the cell cycle regulator p21 (Waf1/Cip1), which upregulates the expression level of enhancer-binding protein a (C/EBPa) and, consequently, promotes the binding of C/EBPa to the HBV promoter; this, in turn, enhances HBV transcription, and thus also viral replication (67) . Nevertheless, it is difficult to evaluate the anthracycline-associated risks for HBV-R as these drugs are often used in combination with other immunosuppressive or chemotherapeutic agents, such as rituximab. Immune checkpoint inhibitors (ICPIs), such as antibodies targeting programmed cell death protein 1/programmed cell death 1 ligand 1 (PD-1/PD-L1) (anti-PD-1: pembrolizumab and nivolumab; anti-PD-L1: atezolizumab, durvalumab, and avelumab) and cytotoxic T-lymphocyte-associated protein 4 (anti-CTLA4: ipilimumab and tremelimumab), have been used as immunotherapy in the treatment of various types of cancer. Pembrolizumab is an FDA-approved agent used to treat relapsed or refractory primary mediastinal large B-cell lymphoma after ≥2 prior lines of therapy. It has been suggested that this class of drugs may give rise to HBV-R. Given that their mechanism of action involves activating the immune system, it seems unlikely that ICPIs induce HBV-R (35); however, sporadic incidents of HBV-R after ICPI administration have been described in case reports and a retrospective cohort study (68) (69) (70) (71) (72) . In the latter, of 114 HBsAg(+) patients given anti-PD-1/PD-L1 antibody therapy, six (5.3%) developed HBV-R in a median time of 18 weeks after treatment initiation (72) . Therefore, given the concern that ICPI treatment may lead to HBV-R, prophylactic antiviral therapy should be an appropriate option for HBVinfected or resolved patients undergoing ICPI therapy. Other biological agents, such as TNF-a inhibitors (infliximab, etanercept, golimumab, and adalimumab), tyrosine kinase inhibitors (TKIs: imatinib, dasatinib, and nilotinib), the Janus kinase (JAK) 1/2 inhibitor ruxolitinib, and the proteasome inhibitor bortezomib, are also thought to be related to HBV-R; however, they are not currently applied to lymphomas (38, 44) . The prevention of HBV-R begins with patient screening. Numerous approaches have been adopted by different organizations and institutions to address the issue of screening for HBV infection before the initiation of immunosuppressive therapy. There is a consensus among various cancer governing bodies that all patients at high risk of HBV-R or those receiving B-cell-depleting therapies should be screened before the initiation of therapy (26, 30, 35) . The AGA, AASLD, EASL, and the American Society of Clinical Oncology (ASCO) screening guidelines are presented in Table 3 (26, 28, 29, 73) . The National Comprehensive Cancer Network (NCCN) Guidelines for B-cell lymphomas suggest that both HBsAg and HBcAb should be tested among NHL patients before the beginning of immunosuppressive treatment, especially that involving anti-CD20 antibody-containing regimens. The baseline HBV DNA burden should be obtained if patients are positive for HBsAg or HBcAb to quickly detect HBV-R and take the appropriate measures (74). Owing to the immunosuppressive agents used in the treatment of NHL that place patients at a very high, high, or moderate risk of HBV-R, all NHL patients should be tested for HBV infection (at least for HBsAg and HBcAb) before the initiation of therapy to minimize the risk of HBV-R and related complications, including mortality. After screening, the next challenge is the management of HBV-R in individuals receiving immunosuppressive agents. There are two strategies targeting HBV-R, namely, antiviral prophylaxis and pre-emptive therapy. Antiviral prophylaxis means treating patients (usually at least 1 week before immunosuppressive therapy) with HBsAg(+) or HBcAb(+) regardless of viral load or whether or not there are clinical symptoms of HBV-R. Preemptive therapy refers to the close surveillance of HBV DNA, in which antiviral therapy begins at the first sign of an increase in the HBV DNA load (26, 74, 75) . The guidelines for management strategies are also presented in Table 3 . In oncological practice, it is usually too late to take measures to deal with HBV-R and schedule treatments are interrupted when HBV-R begins to take shape causing poor outcomes for NHL patients. Consequently, we think that it is appropriate to initiate antiviral prophylaxis before immunosuppressive therapies are given to NHL patients. To date, no antiviral medication has been approved for the prevention of HBV-R. Two types of treatment are available for patients with chronic HBV infection, including nucleoside analogs (NAs) and interferon-alpha. However, interferons are no longer conventionally used to treat lymphoma patients owing to the associated intolerance, adverse effects, and selective effectiveness (46) . Hence, several NAs, such as entecavir (ETV), lamivudine (LMV), adefovir, and tenofovir, are the only effective options available for the treatment of HBV-R among NHL patients. LMV is a cost-effective alternative for HBV treatment and was the first NA approved for this purpose. Consequently, it has been widely used for antiviral prophylaxis among NHL patients (76) . In the first study, none of 30 HBsAg(+) patients with lymphoma undergoing intensive chemotherapy experienced HBV-R in the LMV prophylaxis group, whereas 8 out of 15 patients (53%) in the no-prophylaxis arm had HBV-R (P = 0.002). Survival free from HBV-R-related hepatitis in the group receiving LMV prophylaxis was significantly longer than that of the control group (P = 0.002 on the log-rank test) (77) . In the second randomized trial, among 51 patients undergoing CHOP chemotherapy, HBV-R was detected in 30.8% (95% CI, 14.3-51.8%) of those undergoing LMV prophylaxis vs. 60% (95% CI, 38.7-78.9%) for those not receiving LMV treatment (P = 0.05) (78) . A meta-analysis involving 16 studies reported that the HBV-R rate was significantly lower in patients receiving LMV prophylaxis than in those of the control group (8.6% [11/127] (79) . A recent retrospective study (80) on consecutively enrolled HBsAg(−) and HBcAb(+) NHL patients who received rituximab-based chemotherapy found that none of the patients who were given LMV prophylaxis experienced HBV-R or treatment-related side effects (81) . However, the long-term use of LMV is liable to generate a high rate of drug resistance, especially when used beyond 1 year. The incidence of resistance to LMV has been reported to be as high as 20% in patients treated for more than 1 year with non-immunosuppressive-containing medication (30) ; this incidence escalated dramatically to 30% after 2 years, and then increased exponentially with continued use (76) . The most commonly identified mutation conferring resistance to LMV occurs in the tyrosine-methionine-aspartateaspartate (YMDD) motif of the HBV-DNA polymerase gene (82) . Hence, owing to the low threshold for the generation of resistance to LMV (83) , this drug was replaced by nextgeneration NAs such as ETV or tenofovir that possess a high barrier to resistance, as demonstrated by multiple studies and meta-analyses (28, 80, 84, 85) . ETV and tenofovir disoproxil fumarate are new-generation NAs that have a high barrier to drug resistance and superior viral suppressive capability (23, (86) (87) (88) . A retrospective analysis of HBsAg(+) NHL patients (stage III-IV) who received antiviral prophylaxis with ETV (n = 34) or LMV (n = 89) during chemotherapy suggested that ETV-treated patients had lower rates of hepatitis (5.9 vs. 27.0%, P = 0.007) and HBV-R (0 vs. 12.4%, P = 0.024), as well as fewer interruptions of chemotherapy (5.9 vs. 20.2%, P = 0.042) (89) compared with those treated with LMV. A prospective, randomized, multicenter clinical trial conducted in China that included 121 patients compared the efficacy of ETV (n = 61) and LMV (n = 60) in preventing HBV-R among HBsAg(+) patients undergoing R-CHOP treatment for DLBCL. The results indicated that patients in the ETV group had a markedly lower incidence of HBV-related hepatitis (0 vs. 13.3%, P = 0.003) and HBV-R (6.6 vs. 30%, P = 0.001), as well as fewer interruptions of chemotherapy (1.6 vs. 18.3%, P = 0.002), with respect to those in the LMV group. No difference in terms of incidence of adverse events was identified between these two agents (90) . Another meta-analysis that included 770 lymphoma patients also confirmed that patients with HBsAg(+) receiving LMV prophylaxis during chemotherapy had a significantly higher chance of HBV-R compared with those receiving ETV (OR 5.0, P < 0.001) (80) . In a study conducted to evaluate the effectiveness of tenofovir for the treatment of HBV-R in patients undergoing immunosuppressive treatment, 25 of 38 patients were given tenofovir as prophylaxis, and 13 were administered the drug as pre-emptive therapy. None of the patients receiving tenofovir as prophylaxis developed HBV-R during immunosuppression. In addition, the remaining 13 patients received tenofovir at the first sign of HBV-R and all had a complete biochemical and virological response within 9 months (91). Based on the above observations, ETV or tenofovir (especially ETV) is recommended as the standard agent for the prevention and treatment of HBV-R by NCCN, AASLD, and others as they possess superior viral suppressive ability and a high barrier to resistance (26, 35, 74, 92) . The optimal duration of prophylactic antiviral therapy remains controversial. Data derived from multiple sources indicate that antiviral prophylaxis should last for at least 6 months after the cessation of immunosuppressive therapy, and should be lengthened to 12 months for patients receiving regimens with B-cell-depleting therapies or antiviral prophylaxis (46, 86) . The reason for extending the duration of antiviral prophylaxis use is that immune recovery may take longer, while the immunosuppressive effects of rituximab have been reported to persist for longer than 1 year after the last delivery (35, 93) . HBV-R has also been reported to occur more than 2 years after the completion of rituximab-containing chemotherapy (54, 94) . The AGA, AASLD, EASL, and ASCO guidelines regarding the duration of therapy and monitoring are presented in Table 3 . Additionally, the NCCN guidelines for B-cell lymphomas recommend that monitoring and antiviral prophylaxis should be continued for at least 12 months after the completion of anticancer treatment, and that HBV DNA levels should be tested monthly during treatment, and then every 3 months after completion of antiviral prophylaxis (74). In the clinic, prophylactic antiviral therapy tends to be delivered before or at the onset of anticancer therapy to patients with prior HBV infection, regardless of baseline HBV DNA levels, because HBV-R has been reported to occur even after 1 year of infection. Consequently, close monitoring and longer use of antiviral prophylaxis should be considered, particularly in patients receiving anti-CD20-antibodycontaining therapy (84, (95) (96) (97) . HBV-R in NHL patients undergoing immunosuppressive therapies, especially rituximab-containing treatment, is now a well-recognized and preventable complication in clinical practice. Nevertheless, there is a large knowledge gap in our understanding of this disease process, making HBV-R a vexing and persistent problem. Among NHL patients with chronic HBV infection, HBV-R has gained extensive attention because of the associated significant morbidity and mortality. The immunosuppressive therapies used during the entire NHL treatment period, such as anti-CD20 antibody therapy, glucocorticoid treatment, and HSCT, usually amplify the odds of HBV-R, as mentioned before. Whether to screen patients at risk, stratify patients for risk based on HBV serological status and type of immunosuppression, whether to use prophylaxis or preemptive therapy, and the identification of the optimal antiviral agent and treatment duration remain unresolved issues. Several screening strategies have been proposed by different organizations, namely, risk factor-based, risk-adaptive, and universal screening. HBV testing rates in cancer patients before therapy based on risk factors have been estimated to be low (19-55%) (98-100); however, the morbidity associated with HBV risk factors among patients with cancer may be high (101) . Universal screening is the preferred option for the AGA and the AASLD given the limitations of risk factor-based and risk-adaptive screening. These two organizations recommend universal HBV screening as a reasonable and cost-effective strategy before anticancer therapies are administered to reduce the risk of HBV-R (26, 30) . Accordingly, it is appropriate to test HBsAg, HBcAb, and HBsAb (if available) among all NHL patients to identify and cure HBV-R earlier and provide better clinical outcomes. The current guidelines agree that HBsAg(+) patients or those receiving anti-CD20 antibody therapy or HSCT are at high risk of HBV-R and should be given prophylaxis until after the cessation of anticancer therapy. Among moderate or low-risk HBV-R patients, pre-emptive antiviral therapy initiating at the first sign of HBV-R may also be a decent option. Recent studies and multiple meta-analysis have demonstrated the greater efficacy and lesser drug resistance of ETV and tenofovir as first-line agents for the prevention of HBV-R. The optimal duration of prophylaxis for HBV-R remains unclear. Indeed, Tasuku et al. reported a rare case of a 54-year-old woman diagnosed with DLBCL and HBsAg(−) and HBcAb(+) in whom HBV-R occurred 55 months after the completion of chemotherapy (54) . Owing to the deep immunodepression exerted by rituximab or HSCT, lifelong antiviral treatment may be the better option (23, 102) . In the era of immunotherapy, numerous novel agents have emerged targeting the treatment of NHL and other solid cancers; however, prospective data showing how they interact with the immune system and their relation to HBV-R are limited. Additional, well-designed, prospective studies are needed to allow the stratification of patients at risk of HBV-R as well as a better understanding of the appropriate antiviral therapy and the optimal duration of prophylaxis. With accumulating evidence and experience, it is expected that HBV-R can be avoided, identified, controlled, and, perhaps cured. HL conceived and designed the study and reviewed the manuscript. XC and YW collected the data and wrote the manuscript. PL, WH, and XL revised the manuscript. XC designed and wrote the table. All authors contributed to the article and approved the submitted version. Potential Mechanisms of Hepatitis B Virus Induced Liver Injury Hepatitis B Virus Infection Estimations of Worldwide Prevalence of Chronic Hepatitis B Virus Infection: A Systematic Review of Data Published Between Association of Risk of non-Hodgkin's Lymphoma With Hepatitis B Virus Infection: A Meta-Analysis Chronic Hepatitis B is Associated With an Increased Risk of B-cell non-Hodgkin's Lymphoma and Multiple Myeloma Role of Virological Serum Markers in Patients With Both Hepatitis B Virus Infection and Diffuse Large B-cell Lymphoma Both Chronic HBV Infection and Naturally Acquired HBV Immunity Confer Increased Risks of B-cell non-Hodgkin Lymphoma Hepatitis B and C Viruses and Risk of Non-Hodgkin Lymphoma: A Case-Control Study in Italy Concurrent Infection of Hepatitis B Virus Negatively Affects the Clinical Outcome and Prognosis of Patients With non-Hodgkin's Lymphoma After Chemotherapy Hepatitis B in Patients With Hematological Diseases: An Update Hepatitis B and Risk of Non-Hepatocellular Carcinoma Malignancy Hepatitis Viruses and non-Hodgkin Lymphoma: Epidemiology, Mechanisms of Tumorigenesis, and Therapeutic Opportunities The Association of Hepatitis B Virus Infection With B-Cell Non-Hodgkin Lymphoma -a Review Update of the Statements on Biology and Clinical Impact of Occult Hepatitis B Virus Infection Chemotherapy-Related Reactivation of Hepatitis B Infection: Updates in 2013 Risk of HBV Reactivation in Patients With B-Cell Lymphomas Receiving Obinutuzumab or Rituximab Immunochemotherapy Hepatitis B Reactivation During Cancer Chemotherapy: An International Survey of the Membership of the American Association for the Study of Liver Diseases Rituximab-Associated Hepatitis B Virus (HBV) Reactivation in Lymphoproliferative Diseases: Meta-Analysis and Examination of FDA Safety Reports Hepatitis B Virus Reactivation in Lymphoma Patients With Prior Resolved Hepatitis B Undergoing Anticancer Therapy With or Without Rituximab High Rate of Hepatitis B Viral Breakthrough in Elderly Non-Hodgkin Lymphomas Patients Treated With Rituximab Based Chemotherapy Hepatitis B Reactivation After Chemoimmunotherapy: Screen Before Treatment Prevention of Hepatitis B Reactivation in the Setting of Immunosuppression Long-Term Hepatic Consequences of Chemotherapy-Related HBV Reactivation in Lymphoma Patients Inasl Guidelines on Management of Hepatitis B Virus Infection in Patients Receiving Chemotherapy, Biologicals, Immunosupressants, or Corticosteroids Update on Prevention, Diagnosis, and Treatment of Chronic Hepatitis B: Aasld 2018 Hepatitis B Guidance Asian-Pacific Clinical Practice Guidelines on the Management of Hepatitis B: A 2015 Update American Gastroenterological Association Institute Technical Review on Prevention and Treatment of Hepatitis B Virus Reactivation During Immunosuppressive Drug Therapy Electronic Address Eee, European Association for the Study of the L. Easl 2017 Clinical Practice Guidelines on the Management of Hepatitis B Virus Infection American Gastroenterological Association I. American Gastroenterological Association Institute Guideline on the Prevention and Treatment of Hepatitis B Virus Reactivation During Immunosuppressive Drug Therapy Diagnosis, Prevention and Management of Hepatitis B Virus Reactivation During Anticancer Therapy Covid-19 Induced Hepatitis B Virus Reactivation: A Novel Case From the United Arab Emirates The Global Impact of the COVID-19 Pandemic on the Prevention, Diagnosis and Treatment of Hepatitis B Virus (HBV) Infection Sars-CoV-2 and the Liver: Considerations in Hepatitis B and Hepatitis C Infections Hepatitis B Reactivation Associated With Immune Suppressive and Biological Modifier Therapies: Current Concepts, Management Strategies, and Future Directions NIH Consensus Development Conference: Management of Hepatitis B. Introduction Frequency of Hepatitis B Virus Reactivation in Cancer Patients Undergoing Cytotoxic Chemotherapy: A Prospective Study of 626 Patients With Identification of Risk Factors Reactivation of Hepatitis B Virus Infection in Patients With Hemo-Lymphoproliferative Diseases, and its Prevention Comprehensive Analysis of Risk Factors Associating With Hepatitis B Virus (HBV) Reactivation in Cancer Patients Undergoing Cytotoxic Chemotherapy Hepatitis B Surface Antigen Genetic Elements Critical for Immune Escape Correlate With Hepatitis B Virus Reactivation Upon Immunosuppression Immunosuppression and HBV Reactivation Clinical and Virological Factors Associated With Hepatitis B Virus Reactivation in HBsAg-negative and Anti-HBc Antibodies-Positive Patients Undergoing Chemotherapy and/or Autologous Stem Cell Transplantation for Cancer Hepatitis B Reactivation in the Treatment of Non-Hodgkin Lymphoma Hepatitis B Virus Reactivation: Risk Factors and Current Management Strategies Reconsidering the Management of Patients With Cancer With Viral Hepatitis in the Era of Immunotherapy Optimizing Antiviral Agents for Hepatitis B Management in Malignant Lymphomas Hepatitis B Virus Reactivation and Alemtuzumab Therapy Antiviral Therapies for Managing Viral Hepatitis in Lymphoma Patients A Study of Hepatitis B Virus Reactivation Associated With Rituximab Therapy in Real-World Clinical Practice: A Single-Center Experience Reversal of B-cell Hyperactivation and Functional Impairment Is Associated With HBsAg Seroconversion in Chronic Hepatitis B Patients Rituximab: Mechanism of Action Hepatitis B Reactivation in HBsAgnegative/HBcAb-positive Patients Receiving Rituximab for Lymphoma: A Meta-Analysis Efficient Prophylactic Management of HBV Reactivation by an Information Technology Encoding System: Results of a 6-Year Prospective Cohort Study Hepatitis B Virus Reactivation 55 Months Following Chemotherapy Including Rituximab and Autologous Peripheral Blood Stem Cell Transplantation for Malignant Lymphoma Reactivation of Hepatitis B Virus Following Rituximab-Based Regimens: A Serious Complication in Both HBsAg-positive and HBsAg-negative Patients Hepatitis B Screening, Prophylaxis and Re-Activation in the Era of Rituximab-Based Chemotherapy The Effect of Prophylactic Lamivudine on Hepatitis B Virus Reactivation in HBsAg-Positive Patients With Diffuse Large B-cell Lymphoma Undergoing Prolonged Rituximab Therapy Increased HBV Reactivation Risk With Ofatumumab or Rituximab Recent US Food and Drug Administration Warnings on Hepatitis B Reactivation With Immune-Suppressing and Anticancer Drugs: Just the Tip of the Iceberg? Reactivation of Hepatitis B Virus Infection in Patients With Hematologic Disorders Serum Levels of Hepatitis B Surface and Core Antigens During Immunosuppressive Treatment of HBsAg-positive Chronic Active Hepatitis Management of Hepatitis B Reactivation in Immunosuppressed Patients: An Update on Current Recommendations Steroid-Free Chemotherapy Decreases Risk of Hepatitis B Virus (HBV) Reactivation in HBV-carriers With Lymphoma Hepatitis B Virus DNA Contains a Glucocorticoid-Responsive Element Dexamethasone Stimulates Hepatitis B Virus (Hbv) Replication Through Autophagy Hepatitis B Virus Reactivation and Prophylaxis During Solid Tumor Chemotherapy: A Systematic Review and Meta-Analysis Doxorubicin Activates Hepatitis B Virus Replication by Elevation of p21 (Waf1/Cip1) and C/EBPalpha Expression Hepatitis B Reactivation in a Long-Term Nonprogressor Due to Nivolumab Therapy A Rare Case of Pembrolizumab-Induced Reactivation of Hepatitis B Pembrolizumab-Induced Immune-Mediated Hepatitis and Concurrent Hepatitis B Reactivation in a Patient With Non-Small Cell Lung Cancer HBV-Related Acute Hepatitis Due to Immune Checkpoint Inhibitors in a Patient With Malignant Melanoma Hepatitis B Virus Reactivation in Cancer Patients With Positive Hepatitis B Surface Antigen Undergoing PD-1 Inhibition Hepatitis B Virus Screening and Management for Patients With Cancer Prior to Therapy: Asco Provisional Clinical Opinion Update How I Treat and Monitor Viral Hepatitis B Infection in Patients Receiving Intensive Immunosuppressive Therapies or Undergoing Hematopoietic Stem Cell Transplantation Chronic Hepatitis B: Update Early is Superior to Deferred Preemptive Lamivudine Therapy for Hepatitis B Patients Undergoing Chemotherapy A Revisit of Prophylactic Lamivudine for Chemotherapy-Associated Hepatitis B Reactivation in non-Hodgkin's Lymphoma: A Randomized Trial Effect of Prophylactic Lamivudine for Chemotherapy-Associated Hepatitis B Reactivation in Lymphoma: A Meta-Analysis of Published Clinical Trials and a Decision Tree Addressing Prolonged Prophylaxis and Maintenance Comparison of Entecavir and Lamivudine in Preventing HBV Reactivation in Lymphoma Patients Undergoing Chemotherapy: A Meta-Analysis Lamivudine Prophylaxis Prevents Hepatitis B Virus Reactivation in anti-HBc Positive Patients Under Rituximab for Non-Hodgkin Lymphoma Fatal Postlymphoma Chemotherapy Hepatitis B Reactivation Secondary to the Emergence of a YMDD Mutant Strain With Lamivudine Resistance in a Noncirrhotic Patient Systematic Review: The Effect of Preventive Lamivudine on Hepatitis B Reactivation During Chemotherapy Systematic Review With Network Meta-Analysis: Comparative Efficacy of Oral Nucleos(T) Ide Analogues for the Prevention of Chemotherapy-Induced Hepatitis B Virus Reactivation Meta-Analysis of Prophylactic Entecavir or Lamivudine Against Hepatitis B Virus Reactivation Hepatitis B Virus Screening for Patients With Cancer Before Therapy: American Society of Clinical Oncology Provisional Clinical Opinion Update Comparative Effectiveness of Nucleos(T)Ide Analogues in Chronic Hepatitis B Patients Undergoing Cytotoxic Chemotherapy Clinical Profile and Efficacy of Antivirals in Hepatitis B Virus Reactivation, in Patients With Cancer Receiving Chemotherapy Comparison of Entecavir and Lamivudine in Preventing Hepatitis B Reactivation in Lymphoma Patients During Chemotherapy Entecavir vs Lamivudine for Prevention of Hepatitis B Virus Reactivation Among Patients With Untreated Diffuse Large B-cell Lymphoma Receiving R-CHOP Chemotherapy: A Randomized Clinical Trial The Role of Tenofovir in Preventing and Treating Hepatitis B Virus (HBV) Reactivation in Immunosuppressed Patients. A Real Life Experience From a Tertiary Center Comparative Efficacy of Oral Nucleotide Analogues for the Prophylaxis of Hepatitis B Virus Recurrence After Liver Transplantation: A Network Meta-Analysis Late Hepatitis B Virus Reactivation After Lamivudine Prophylaxis Interruption in an anti-HBs-positive and anti-HBc-negative Patient Treated With Rituximab-Containing Therapy Reactivation of Occult Hepatitis B Virus Infection 27 Months After the End of Chemotherapy Including Rituximab for Malignant Lymphoma Persistent Risk of HBV Reactivation Despite Extensive Lamivudine Prophylaxis in Haematopoietic Stem Cell Transplant Recipients Who Are anti-HBc-positive or HBV-negative Recipients With an anti-HBc-positive Donor Hepatitis B Virus Reactivation After Withdrawal of Prophylactic Antiviral Therapy in Patients With Diffuse Large B Cell Lymphoma Delayed HBV Reactivation in Rituximab-Containing Chemotherapy: How Long Should We Continue Anti-Virus Prophylaxis or Monitoring HBV-DNA? Low Rates of Hepatitis B Virus Screening at the Onset of Chemotherapy Trends in Hepatitis B Virus Screening at the Onset of Chemotherapy in a Large US Cancer Center Poor Recognition of Risk Factors for Hepatitis B by Physicians Prescribing Immunosuppressive Therapy: A Call for Universal Rather Than Risk-Based Screening Models to Predict Hepatitis B Virus Infection Among Patients With Cancer Undergoing Systemic Anticancer Therapy: A Prospective Cohort Study Lamivudine Treatment for Reverse Seroconversion of Hepatitis B 4 Years After Allogeneic Bone Marrow Transplantation