key: cord-0964158-i5c2rnda authors: Bouquegneau, Antoine; Huart, Justine; Lutteri, Laurence; Erpicum, Pauline; Grosch, Stéphanie; Résimont, Guillaume; Wiesen, Patricia; Rousseau, Anne-Françoise; Bovy, Christophe; Krzesinski, Jean-Marie; Thys, Marie; Lambermont, Bernard; Misset, Benoît; Pottel, Hans; Darcis, Gilles; Cavalier, Etienne; Jouret, François; Delanaye, Pierre title: Survivors of COVID-19 mostly recover from tubular proteinuria and acute kidney injury after hospital discharge date: 2021-06-05 journal: J Nephrol DOI: 10.1007/s40620-021-01075-1 sha: b87e7a481f26e9baa108b43d1f0d868fef0e14dd doc_id: 964158 cord_uid: i5c2rnda nan remain unknown. In the present observational single-center study, we report the follow-up (F-U) of both proteinuria and estimated glomerular filtration rate (eGFR) in COVID-19 patients post hospital discharge [3] . All data were anonymized and the study had been approved by the Ethics Committee of ULiège Academic Hospital. Patients older than 18 years of age admitted to Liège Academic Hospital between March 28th and April 30th, 2020 with PCR-confirmed COVID-19, for whom data concerning total proteinuria and/or urine α 1 -microglobulin were available were included in our study. A detailed description of the methodology has been previously published [3] . Briefly, serum creatinine and total proteinuria before COVID-19 were also collected when available. Total proteinuria (expressed in mg/g of urine creatinine), urine α 1microglobulin and β 2 -microglobulin were determined on a random spot. Proteinuria was staged according to the KDIGO (Kidney Disease Improving Global Outcomes). Urine α 1 -microglobulin was expressed in mg/g of urine creatinine with a pathological threshold at > 15 mg/g. Urine β 2 -microglobulin was expressed in mg/L, with a pathological threshold at > 0.19 mg/L. Hematuria was defined by the presence of more than 10 red blood cells per field. AKI during hospitalization (Hosp) was based on the KDIGO definition (after considering the serum level of creatinine at admission as the baseline value). Decreased kidney function was based on the CKD-Epidemiology equation (CKD-EPI) and using an age-calibrated definition. The same parameters were then followed after hospital discharge until August 28th, 2020 in the context of a global health follow-up of hospitalized COVID-19 patients established by the hospital. Data are expressed as median with quartiles. Comparison Antoine Bouquegneau and Justine Huart equally contributed as first authors. * Pierre Delanaye pierre_delanaye@yahoo.fr 1 Service de Dialyse, Department of Nephrology-Dialysis-Transplantation, CHU Sart Tilman, University of Liège (CHU ULiege), Liège, Belgium of independent and paired groups were performed using the Mann-Whitney U/Chi-square tests and Wilcoxon signed rank test/McNemar tests, respectively [3] . We included 153 hospitalized COVID-19 patients for whom urine data were available after a median period of 3 (2; 5) days post admission (Hosp). Among the 153 patients, 30 patients died (mortality rate of 19.6%). Of the 123 survivors, follow-up (F-U) urine analyses were available for 72 patients (58.5%) (Flow chart in Supplementary Figure S1 ). The median period between the day of hospital discharge and the last day of follow-up was 51 (19; 93) days. The median age of our 72-patient cohort was 64 (58-75) years old, with a M/F gender ratio of 37% (Supplementary Table S1 ). Median proteinuria levels (n = 72) at Hosp and F-U were 419 (239; 748) and 79 (47; 129) mg/g, respectively (p < 0.0001) ( Table 1 ). During hospitalization, 15% (n = 11) had category 1 proteinuria, 43% (n = 31) had category 2 and 42% (n = 30) had category 3. At F-U, 83% (n = 60) had category 1 proteinuria, 11% (n = 8) had category 2 and 6% (n = 4) had category 3. Of important note, 2 of these latter 4 patients already had proteinuria prior to COVID-19, in the context of chronic uropathy (n = 1), diabetic nephropathy (n = 1), and one patient had a rheumatological disorder treated by cyclosporine and maintained by methotrexate. At Hosp and F-U, the median concentrations of urinary α 1microglobulin (n = 66) were 50 (25; 81) and 8 (0; 19) mg/g, respectively (p < 0.0001). The α 1 -microglobulin concentration was higher than 15 mg/g in 88% and 29% of patients at Hosp and F-U, respectively (Table 1) . At Hosp and F-U, the median concentrations of urinary β 2 -microglobulin (n = 60) were 0.92 (0.41; 4.52) and 0.00 (0.00; 0.00) mg/L, respectively (p < 0.0001). Urine β 2 -microglobulin was higher than 0.19 mg/L in 82% and 13% of patients at Hosp and F-U, respectively. Data on hematuria were available at F-U in 51 patients. At Hosp and F-U, 19% and 12% had hematuria, respectively (ns). Hosp eGFR was lower than F-U eGFR: 81 (62; 92) versus 87 (66; 98) mL/min/1.73m 2 (p = 0.0222). Still, decreased renal function was observed in 10/72 (14%) patients at F-U compared to Hosp values. Among these 10 patients, 5 already presented with decreased renal function before COVID-19. In the remaining 5 patients with normal renal function before COVID-19, 4 developed category 3 AKI during hospitalization and 3 transiently required renal replacement therapy (RRT). Note that dialysis was withdrawn before hospital discharge in all 3 patients who required RRT during hospitalization. Our F-U observations suggest that, although COVID-19 is associated with a high prevalence of tubular proteinuria and AKI, most patients (~ 80%) recover statu quo ante within ~ a month post hospital discharge. Patients with persistent proteinuria at F-U already had previous clinical conditions favoring chronic proteinuria and/or suffered from the most severe AKI. The statistically significant, but probably not clinically relevant, difference between eGFR at Hosp and F-U remains in the range of normality. Patients with COVID-19 often presented with fever and gastrointestinal symptoms that may account for volume depletion and explain the slightly lower level of eGFR at Hosp compared to F-U. The main limitation of our study is the retrospective analysis of the data. Moreover, although we lack measurements regarding urine albumin (not reimbursed by the Belgian health system), protein immunofixation was available in more than 50% of patients at Hosp and F-U and clearly demonstrated a pattern of mixed proteinuria. Finally, the design of the current study did not allow us to formally distinguish acute tubular necrosis caused by septic conditions, potential toxicity of drugs or contrast agents during hospitalization or cytopathic lesions caused by SARS-CoV-2 itself, but the fact is that long-term consequences seem limited. The present study confirms first reports suggesting that long-term renal sequelae of COVID-19 appear uncommon [4, 5] . Similarly to other severe infectious diseases, a specific follow-up by nephrologists is recommended for survivors of COVID-19 who developed severe AKI and/or heavy proteinuria (~ nephrotic range). These reassuring data need to be confirmed in larger cohorts. The online version contains supplementary material available at https:// doi. org/ 10. 1007/ s40620-021-01075-1. Acute kidney injury in patients hospitalized with COVID-19 Kidney disease is associated with in-hospital death of patients with COVID-19 Proteinuria in COVID-19: prevalence, characterization and prognostic role High rates of long-term renal recovery in survivors of COVID-19-associated acute kidney injury requiring kidney replacement therapy AKI in hospitalized patients with COVID-19 We sincerely thank all physicians and nurses who take care of COVID-19 patients in the clinical wards and intensive care units. Conflict of interest All the authors declare that they have no conflict of interest involving the work under consideration for publication.Ethical approval All data were anonymized and the study had been approved by the Ethics Committee of ULiège Academic Hospital.Informed consent For this study, consent is not required.