key: cord-0966111-9z2zw44u authors: Diffley, John F.X. title: Author's overview: identifying SARS-CoV-2 antiviral compounds date: 2021-07-16 journal: Biochem J DOI: 10.1042/bcj20210426 sha: b879d182821c5cbb701834cdaa52bf07af155abe doc_id: 966111 cord_uid: 9z2zw44u In response to the COVID-19 pandemic, we began a project in March 2020 to identify small molecule inhibitors of SARS-CoV-2 enzymes from a library of chemical compounds containing many established pharmaceuticals. Our hope was that inhibitors we found might slow the replication of the SARS-CoV-2 virus in cells and ultimately be useful in the treatment of COVID-19. The seven accompanying manuscripts describe the results of these chemical screens. This overview summarises the main highlights from these screens and discusses the implications of our results and how our results might be exploited in future. In response to the COVID-19 pandemic, we began a project in March 2020 to identify small molecule inhibitors of SARS-CoV-2 enzymes from a library of chemical compounds containing many established pharmaceuticals. Our hope was that inhibitors we found might slow the replication of the SARS-CoV-2 virus in cells and ultimately be useful in the treatment of COVID-19. The seven accompanying manuscripts describe the results of these chemical screens. This overview summarises the main highlights from these screens and discusses the implications of our results and how our results might be exploited in future. Coronaviruses encode multiple enzymes important for viral replication [1] , and any of these could be attractive drug targets. We identified inhibitors of seven enzymes, described in the accompanying papers. Table 1 summarises the viral enzymes that control SARS-CoV-2 replication, and the best hits obtained for each that we screened. Our screens identified several drugs that might be repurposed to treat COVID-19. Suramin, which we identified as inhibiting both nsp13 RNA helicase and nsp12/7/8 RdRp, is used to treat African sleeping sickness and river blindness [2, 3] . Interestingly, suramin also affects the growth of a wide range of viruses, in part by interfering with virus-receptor interactions [2, [4] [5] [6] . Two of the nsp14 cap methyltransferase inhibitors are also clinically relevant. Trifluperidol is used in the treatment of mania and schizophrenia. Lomeguatrib is an inhibitor of O 6 -methylguanine-DNA-methyltransferase [7] and has been used to enhance the effects of DNA alkylating agents including Temozolomide. A better understanding of the side effects associated with the doses of these drugs required to suppress viral growth is needed before considering them for clinical use. Other inhibitors are further from the clinic. PF-03882845, another nsp14 cap methyltransferase inhibitor, is a non-steroidal mineralocorticoid antagonist [8] which has been shown to be orally bioavailable and well-tolerated in humans. GSK-650394, which we found as an RdRp inhibitor, is an inhibitor of serum and glucocorticoid-activated kinase (SGK1) [9] . Interestingly, GSK-650394 has previously been shown to inhibit Influenza virus replication in cell models [10] . Dihydrotanshinone I was the best nsp3/PLpro inhibitor we found and also inhibited viral growth in vitro. Dihydrotanshinone I is a naturally occurring compound from the plant Salvia miltiorrhiza and did not exhibit cytotoxicity in our experiments. Further tests of efficacy and toxicity in animal models might determine whether dihydrotanshinone I has potential in the clinic. Our screens also identified compounds that might be leads for further drug development. For example, by optimising the peptide sequence of a peptidomimetic Caspase inhibitor we found in the screen, we were able to generate an inhibitor of nsp5 Main protease with an IC 50 of less than 1 nanomolar. Though this drug (Z-AVLD-FMK) was relatively ineffective in preventing virus replication in cell culture, it represents a good starting point for developing better, perhaps more bioavailable, inhibitors. It may ultimately be important to obtain atomic resolution structures of each of the enzymes with their inhibitors to inform the chemistry required to improve inhibitor efficacy. Our inhibitors, taken together, comprise a nascent 'chemical toolbox' for studying SARS-CoV-2. Such a toolbox could be used to study the roles of these enzymes in the intracellular phase of the coronavirus cycle, identifying major vulnerabilities. Moreover, by determining the effects of combinations of inhibitors on viral growth, novel approaches to combination therapies may be identified. In this regard, the efficacy of the nsp14 methyl transferase inhibitors was enhanced by combination with remdesivir, suggesting an interesting possibility for a combination therapy. Nsp14/10 has been hypothesised to act as a 'proofreading exonuclease' during virus replication [11] [12] [13] . Interestingly, nsp14/10 exonuclease inhibitors did not exhibit any additive effects with remdesivir. Remdesivir acts as a delayed chain terminator, terminating three nucleotides after its incorporation [14, 15] , and thus may evade proofreading by nsp14/10. It will be interesting to examine this possibility with the purified proteins and to determine if nsp14/10 inhibitors make direct chain terminators more effective in inhibiting viral replication. Many of the enzymes we examined are highly conserved in coronaviruses. It will be important to test these inhibitors across a wide range of coronaviruses. By interfering with the intracellular phase of the virus life cycle, these inhibitors may cause accumulation of partially replicated, or partially capped cytoplasmic RNAs. Such structures are known to elicit cellular antiviral responses, so the inhibitors we have identified may, in addition to directly inhibiting viral replication, also facilitate cellular responses to viral infection. Further work is needed to investigate this possibility. There are many other classes of viruses that have the potential to cause serious health problems in the future. Structural viral coat proteins tend to evolve rapidly, making it difficult to develop vaccines before new Not screened n/a n/a n/a 1 Cytotoxic compounds. threats emerge; however the enzymology involved in viral replication evolves more slowly, suggesting that inhibitors with broad efficacy across viral groups could be developed and would be useful as frontline defence while vaccines are developed. We hope that this project will help convince governments to coordinate efforts to develop antivirals to virus groups before they become problems. The nonstructural proteins directing coronavirus RNA synthesis and processing years of suramin Human African trypanosomiasis Suramin inhibits SARS-CoV-2 infection in cell culture by interfering with early steps of the replication cycle The approved pediatric drug suramin identified as a clinical candidate for the treatment of EV71 infection-suramin inhibits EV71 infection in vitro and in vivo The suramin derivative NF449 interacts with the 5-fold vertex of the enterovirus A71 capsid to prevent virus attachment to PSGL-1 and heparan sulfate Lomeguatrib, a potent inhibitor of O6-alkylguanine-DNA-alkyltransferase: phase I safety, pharmacodynamic, and pharmacokinetic trial and evaluation in combination with temozolomide in patients with advanced solid tumors PF-03882845, a non-steroidal mineralocorticoid receptor antagonist, prevents renal injury with reduced risk of hyperkalemia in an animal model of nephropathy Development of a small-molecule serum-and glucocorticoid-regulated kinase-1 antagonist and its evaluation as a prostate cancer therapeutic Serum-and glucocorticoid-regulated kinase 1 is required for nuclear export of the ribonucleoprotein of influenza A virus RNA 3 0 -end mismatch excision by the severe acute respiratory syndrome coronavirus nonstructural protein nsp10/nsp14 exoribonuclease complex Infidelity of SARS-CoV Nsp14-exonuclease mutant virus replication is revealed by complete genome sequencing Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency Mechanism of SARS-CoV-2 polymerase stalling by remdesivir The author declares that there are no competing interests associated with this manuscript.