key: cord-0977356-rl19x0rx authors: Beverina, Ivo; Borotto, Erika; Novelli, Chiara; Radrizzani, Danilo; Brando, Bruno title: Iatrogenic anaemia and transfusion thresholds in ICU patients with COVID-19 disease at a tertiary care hospital date: 2021-01-22 journal: Transfus Apher Sci DOI: 10.1016/j.transci.2021.103068 sha: 5da266d2786b92c095cdebc2c58619d984b551e8 doc_id: 977356 cord_uid: rl19x0rx Background Patients with severe COVID-19 disease frequently develop anaemia as the result of multiple mechanisms and often receive transfusions. The aims of this study were to assess the impact of repeated blood samplings on patients’ anaemic state using standard-volume tubes, in comparison with the hypothetical use of low-volume tubes and to evaluate the transfusion policy adopted. Study design and methods Transfusion data of mechanically ventilated non-bleeding patients with COVID-19 disease hospitalized in ICU for a minimum of 20 days were recorded. The total volume of blood drawn for samplings with standard-volume tubes and the corresponding red blood cell mass (RBCM) removed during hospitalization for each patient were calculated and compared with the hypothetical use of low-volume tubes. Results Twenty-four patients fulfilled the inclusion criteria. Ten patients were anaemic at ICU admission (41.7%). Overall, 6,658 sampling tubes were employed, for a total of 16,786 mL of blood. The median RBCM subtracted by blood samplings per patient accounted for about one third of the total patients’ RBCM decrease until discharge. The use of low-volume tubes would have led to a median saving of about one third of the drawn RBCM. Eleven patients were transfused (45.8%) at a mean Hb value of 7.7 (± 0.5) g/dL. Conclusion The amount of blood drawn for sampling has a significant role in the development of anaemia and the use of low-volume tubes could minimize the problem. Large high-powered studies are warranted to assess the more appropriate transfusion thresholds in non-bleeding critically ill patients with COVID-19 disease. The development of anaemia is common in patients during hospitalization in Intensive Care Unit (ICU) [1] . Several causes can lead to a reduction of the Red Blood Cell Mass (RBCM) in this setting. Bleeding caused by the underlying disease or induced by invasive procedures, impaired iron-restricted hematopoiesis secondary to inflammation [2] and reduced red blood cell lifespan are known as the most frequent causes [3] . Moreover, the daily amount of blood drawn for the frequent blood testing can represent a worsening factor. With the aim to reduce, inter alia, the impact of iatrogenic anaemia, the Society of Critical Care Medicine and the American Association of Blood Banks support the Choosing Wisely campaign: "Do not order diagnostic test at regular intervals (such as every day), but rather in response to specific questions" [4] and "Don't perform serial blood counts on clinically stable patients" [5] , respectively. After January 30 th 2020, when the first two imported cases of SARS-CoV-2 infection in Italy were documented, a rapid increase in admissions of patients with severe acute respiratory syndrome to ICU has been observed during the following weeks. Overall, from March 8 th until May 20 th 2020 eighty-four SARS-CoV-2 patients were treated in ICU at the tertiary care Legnano General Hospital. Our first aim was to retrospectively evaluate the amount of RBCM drawn for blood testing during the ICU stay as a causative factor of anaemia, and the consequent transfusion approach. Since standard-volume sampling tubes were currently in use at our hospital, the second aim of this study was to estimate the theoretical RBCM that would have been spared with the use of reduced-volume sampling devices and the potential changes in the consequent transfusion policy. J o u r n a l P r e -p r o o f After exclusion of patients hospitalized in ICU for less than twenty days, with a non-invasive respiratory support only, those with overt bleeding and patients who died, twenty-four patient fulfilled the inclusion criteria and represented our study cohort. The baseline and transfusion data of patients admitted to ICU with SARS-CoV-2 infection from March 8 th to May 20 th 2020 were retrospectively collected. The analysis was restricted to patients undergoing orotracheal intubation and discharged alive from ICU after at least 20 days. In ICU routine blood testing were usually drawn with a closed blood conservation device (VAMP adult system, Edwards Lifescience, Irvine, CA, USA), with the aim to reduce the waste of patients' blood. Anthropometric, hematological and transfusion data were recorded, along with the number and type of tubes used for tests throughout the ICU stay. The total volume of blood drawn with standard Vacutainer TM tubes, Bactec TM culture vials (Becton Dickinson, Milan, Italy), SafePICO TM arterial blood gas syringes (Radiometer, Brønshøj, Denmark) and the corresponding RBCM removed during hospitalization for each patient were calculated, based on the daily record of ordered testing. The resulting parameters were then recalculated in a hypothetical scenario in which commercially available low-volume sampling tubes intended for use in adults were employed (S-Monovette ® , Sarstedt AG & Co., Nümbrecht, Germany). Both the actual and the simulated RBCM losses were evaluated as potential triggers for transfusion, taking into account that one unit of Packed Red Blood Cells (PRBC) for transfusion contains an average of 170 grams of RBCM. The Nadler's formula was used to calculate the patients' total blood volume. Baseline patient RBCM was calculated multiplying the total blood volume by the baseline hematocrit. The drawn RBCM was calculated by multiplying the tube numbers and volumes by the corresponding daily hematocrit. The RBCM at discharge from ICU was calculated for each patient. In case of transfusion, the corresponding administered RBCM was subtracted from the final calculated value. J o u r n a l P r e -p r o o f Data from the 24 eligible patients were summarized in Table 1 . Median length of stay was 29 days (min 20max 43), male/female ratio was 1.6 (15/9) and median age 65. Nineteen patients (79%) were overweight, with BMI > 25 Kg/m 2 and 41.7% (10/24) were anaemic at ICU admission, as defined by World Health Organization criteria in adults and non-pregnant women [6] (i.e. Haemoglobin < 13.0 g/dL for men and < 12.0 g/dL for women). One patient had a previous episode of myocardial infarction. The mean Hb level decreased progressively throughout the ICU stay ( Figure 1 ). Among nonanaemic patients, the development of anaemia started at a median of three days (min 2max 9). Overall, 6,658 sampling tubes were employed, for a total 16,786 mL of blood drawn. The distribution of tubes used for blood testing is reported in Table 2 . Median blood drawn volume per patient per day was 21.7 mL, with a total median 719 mL loss throughout the ICU stay. Overall, the median RBCM subtracted by blood samplings accounted for about one third of the total patients' RBCM decrease until discharge: -31.1% [21.7 -49.5] , with a nadir of -75.5% in one case. A simulation was run with the hypothetical usage of commercially available low-volume sampling tubes, as detailed in Table 2 . Blood gas analysis requires an irreducible sampling of 1 mL, as well as blood cultures with Bactec™ vials cannot be performed in lower-volume containers. Overall, median differences between standard and low-volume tubes in terms of mL/day were 21.7 [18.7 -26.7 ] versus 14.0 [11.3 -18] , respectively, p < 0.001, corresponding to a median decrease in blood drawn per day of 35.5% ( Figure 3 ). The differences in RBCM decrease per patient using the low-volume tubes ranged from 28.5% to 45.9% with a median value of 33.3%. Focusing on tubes for blood cell count, coagulation and clinical chemistry, that have their respective low-volume counterparts, the decrease in drawn blood volume would have been 49.2% (overall 5,618.2 mL versus 11,054 mL). The comparison between the actual and the simulated RBCM drawn for each patient is shown in In this study we analyzed data of 24 patients admitted to ICU and undergoing orotracheal intubation due to Acute Respiratory Distress Syndrome (ARDS) induced by SARS-CoV-2 infection. The reasons for restricting our evaluation to surviving and non-acutely bleeding patients were the gathering of a homogeneous cohort of subjects with an ICU stay of at least twenty days, excluding the confounding effect of ongoing hemorrhages on RBCM loss calculations. The COVID-19 disease outbreak had a tremendous impact on the Italian health system in general, especially putting in strain the ICUs' capacity of many hospitals. Moreover, in the first week of March 2020 the epidemic caused the crisis of donated blood inventory, although mitigated by a reduction in blood product consumption (e.g. breaking non urgent admissions and postponing programmed surgery) [7] . Hospital-acquired anaemia is a common condition in critical patients admitted to ICU and is associated with worse clinical outcomes [8] . Anaemia in SARS-CoV-2 infection seems to be correlated with illness severity and [9] is the results of a multiple pathogenetic mechanisms [10] . The cytokine storm, induced by the virus in the more severe cases, leads to an impaired iron utilization through the interference on the hepcidin/ferroportin axis, that ultimately causes the sequestration of iron into storage cells [11] . In our cohort, 79% of patients were overweight (i.e. BMI > 25 Kg/sqm) and obesity is also a recognized cause of impaired iron absorption [12] . Moreover, data are accumulating about the development of hemolytic autoimmune anaemia with a positive direct anti-globulin test in some infected patients [13] [14] [15] . Inflammation can also lead to a reduced RBC lifespan, through a membrane alteration [16, 17] . Finally, a direct infection of precursor cells by the virus itself could be a further mechanism inducing anaemia [18] . Iatrogenic anaemia is a well-recognized cause of anaemia in patients in ICU [1, 19] and the minimization of clinically unnecessary blood sampling is a recommended practice through all the three pillars of the patient blood management approach [20] [21] [22] . However, in the context of acute COVID-19 disease the frequency and the amount of blood samplings have been overlooked as an important factor causing the continuous drop in patients' haemoglobin and the need of blood transfusion throughout the ICU stay. In our studied cohort a total of more than sixteen liters of blood was subtracted from 24 patients over about 20 days and the median value of RBCM drawn by blood sampling accounted for about a third of patients' total RBCM decrease until discharge, with a nadir of -75% in one patient. In five cases the amount of RBCM subtracted by phlebotomy approached or exceeded the corresponding volume of two RBC units. In a recent study performed in a mixed ICU population of 7,273 patients [23] , the authors found a median daily blood loss due to laboratory testing of 25 mL [IQR 14 -43] . Interestingly, in the same cohort the cumulative blood loss from day 2 to 7 was independently associated with the risk of transfusion. The daily median volume of blood drawn in our study population approached that of the abovementioned study, i.e. 21.7 mL , with a difference between transfused and nontransfused patients (2.7 mL) that reached the statistical significance. Although this difference may appear small, nevertheless similar values were associated with a doubling of the odds of transfusion in a previous study [24] . Various approaches have been recommended and put into practice with the aim to prevent and minimize iatrogenic anaemia in ICU [21, [25] [26] [27] [28] . In accord with our simulation, the use of lowvolume tubes would have reduced the RBCM drawn of about one third. However, it is not possible to predict accurately the efficacy of this intervention on transfusion rate, and studies evaluating the impact of reducing blood samplings on transfusion requirements have given conflicting results [26, 29] . Nevertheless, in a recent systematic review based on twenty-one studies performed in J o u r n a l P r e -p r o o f mixed settings, the Authors concluded that the routine use of low-volume tubes as blood-sparing devices can be recommended [30] . The mean amount of blood needed for tests, blood culture excluded, by modern analyzers range between 100 to 200µL, about twenty-fold more than blood collected by standard tubes (i.e. 2 -5 mL) . This leads to a more than 90% of blood discarded [31] . In the face of this evidence, the extended use of low-volume tubes should be advisable. Despite we have selected a group of non-bleeding patients, the overall transfusion rate was rather high (48.5%). In a study by Chant et al., the authors reported a transfusion rate of 62% in a mixed ICU population. However, the reason for transfusion was active bleeding in 17% of cases, whereas in 26% of cases the reason of the transfusion was not identifiable [24] . In absence of specific haemoglobin thresholds tailored for patients with COVID-19 disease, transfusion therapy with RBC can be safely performed according to current guidelines recommending a restrictive approach in critically ill patients [32] . However, as recently reaffirmed [33, 34] , transfusion is a complex decision process. A transfusion policy guided only by strict transfusion thresholds could be harmful for the frail ICU population affected by a possible impairment of peripheral perfusion and limited oxygen delivery and/or extraction. Nevertheless, data based on large observational studies can be unaffected by the biases of randomized clinical trials, thus possibly giving more detailed answers on patients' outcomes depending on transfusion policy. Moreover, despite current guidelines, as shown by a recent worldwide audit, the transfusion thresholds applied in "real-life" seems slightly higher than recommended (Hb 8.3 ± 1.7 g/dL) [35] . In our cohort, the mean haemoglobn at transfusion was 7.7 g/dL (SD ± 0.5). Nine out eleven patients transfused (81.8%) were older than 60 years, and possibly with age-dependent comorbidities (i.e. cardiorespiratory insufficiency), conditioning less restrictive transfusion thresholds. [36] . In absence of sound evidence, more recent guidelines in critically ill patients [37] give no or weak transfusion recommendations in a number of acute clinical conditions that are J o u r n a l P r e -p r o o f represented in our series. Moreover, we can justify the slightly liberal approach that was applied as a consequence of facing a new, clinically unknown and lethal disease. Actually, a just published retrospective study [38] comparing patients with ARDS transfused at low (< 8.0 g/dL) versus high hemoglobin threshold (< 10.0 g/dL), although did not found differences in 28-days mortality and within 28-days ECMO-free, sedation-free and organ dysfunction-free composites, demonstrated a delayed weaning from mechanical ventilation in the low threshold group (p=0.02). Our study has some limitations. First of all, the number of cases is low to draw definitive conclusions. The reasons for repetitive withdrawals by ICU's attending physician was not strictly protocol-based, leading to a possible over-sampling respect to a real clinical usefulness. Furthermore, we could not verify that the blood conservation device was always used, with a possible underestimation of iatrogenic blood loss in some cases. In this regard, our decision to focus on RBCM was aimed at obtaining an haemodilution-independent parameter, and this could be a point of strength of the present study. Lastly, any theoretical simulation based on calculations is intrinsically limited. The amount and frequency of blood sampling considerably account for the development of anaemia in COVID-19 patients in ICU and the use of low-volume sampling tubes could minimize the progressive fall of haemoglobin and possibly the risk of transfusion. Larger high-powered studies are warranted to assess the more appropriate transfusion thresholds in non-bleeding critically ill patients with COVID-19 disease. All the Authors declare no conflicts of interest. The study was not funded. Authorship contribution IB: study design, data collection, analysis and interpretation; manuscript writing; CN data collection, manuscript comment and review; EB, DR, BB: manuscript comment and review. All the authors have read and approved the final manuscript. 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