key: cord-0981273-iufcx7i3 authors: Coelho, Rocío M.; Periolo, Natalia; Duhalde, Carolina Perez; Alonso, Daniel O.; Bellomo, Carla M.; Corazza, Marisa; Iglesias, Ayelén A.; Martinez, Valeria P. title: Hantavirus Pulmonary Syndrome in a COVID-19 Patient, Argentina, 2020 date: 2022-04-03 journal: Emerg Infect Dis DOI: 10.3201/eid2804.211837 sha: d8d37df0e2d364160af9009b5bb138e8b6cab1ce doc_id: 981273 cord_uid: iufcx7i3 We describe a patient in Argentina with severe acute respiratory syndrome coronavirus 2 infection and hantavirus pulmonary syndrome (HPS). Although both coronavirus disease and HPS can be fatal when not diagnosed and treated promptly, HPS is much more lethal. This case report may contribute to improved detection of co-infections in HPS-endemic regions. T he current coronavirus disease (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in substantial illness and death rates worldwide. Orthohantaviruses are zoonotic viruses responsible for another severe respiratory infectious disease in the Americas, hantavirus pulmonary syndrome (HPS). Although humans generally become infected with HPS through inhaling excreta generated by infected rodents, person-to-person transmission has been well documented in Argentina and Chile (1) (2) (3) . Humans become infected with SARS-CoV-2 and orthohantaviruses in similar ways, through inhaling contaminated aerosols, and can have onset of similar respiratory syndromes. Despite these similarities, the incubation period is shorter in COVID-19 patients (2-14 days) than in HPS patients (7-45 days) . Furthermore, at the time the case we describe was reported, the cumulative case-fatality rate for CO-VID-19 in Argentina was 2.7% (4); for HPS, it was 22%-40% (5). HPS is characterized by the onset of symptoms such as fever, myalgia, cough, dyspnea, diarrhea, and sweating. Rapid progression to shock or respiratory distress can occur within hours. Symptom-based We describe a patient in Argentina with severe acute respiratory syndrome coronavirus 2 infection and hantavirus pulmonary syndrome (HPS). Although both coronavirus disease and HPS can be fatal when not diagnosed and treated promptly, HPS is much more lethal. This case report may contribute to improved detection of co-infections in HPS-endemic regions. therapy with oxygen and ventilatory or circulatory support is needed (6, 7) . We describe a case of SARS-CoV-2 and Andes virus co-infection in central Argentina. The patient, a 22-year-old woman without relevant pathologic records, sought care at a local hospital in November 2020 for fever, headache, myalgia, and gastrointestinal manifestations. A nasopharyngeal swab sample tested positive for SARS-CoV-2 by reverse transcription PCR at the Instituto Biológico "Tomás Perón" (Appendix, https://wwwnc.cdc.gov/EID/ article/28/4/21-1837-App1.pdf). Five days after the onset of fever, the patient's clinical status worsened, and she was admitted to the hospital. Clinical laboratory findings at admission indicated thrombocytopenia, high leukocyte count, lymphopenia, and elevated hepatic enzymes (Appendix). Computed tomography revealed bilateral pleural effusion associated with interstitial infiltration, and capillary filtration with slight peripheral pulmonary ground-glass opacity ( Figure) . Within a few hours after admission, the patient had onset of marked respiratory distress. She was then referred to the intensive care unit for orotracheal intubation and treated with ampicillin/sulbactam and azithromycin. The epidemiologic investigation established that the patient resided in a hantavirus-endemic area. Consequently, HPS was suspected, despite the COVID-19-positive diagnosis. According to the confirmation criteria used by the Hantavirus National Reference Laboratory (8), Andes virus infection was confirmed by the detection of specific IgM and IgG by ELISA and genomic viral RNA by quantitative reverse transcription PCR in blood (Appendix). Three days after the co-infection was confirmed, the patient was extubated and progressed favorably. Twenty days after onset of symptoms, she was discharged from the hospital. To determine the viral genotype of Andes virus, we conducted a nucleotide sequence analysis from 2 partial fragments of viral small (496-bp) and medium (611-bp) segments, and we submitted the sequences obtained to GenBank (accession nos. OL840325 and OL840326). The highest nucleotide identities matched previous published sequences corresponding to Plata genotype of Andes virus (GenBank accession nos. EU564720 [96% identity] and AY101185 [97.8 identity]). This viral genotype is one of the prevalent pathogenic orthohantaviruses circulating in central Argentina and Uruguay (9) . Because the incubation period for HPS is longer than that for COVID-19, we might speculate that hantavirus infection occurred before coronavirus infection. The respiratory distress syndrome appeared 5 days after the onset of fever, which coincided with the characteristic prodromal period described for HPS. This condition, during the incubation period of HPS, could have induced a higher susceptibility to COVID-19. Because HPS can evolve rapidly to respiratory failure in most patients with severe disease, resulting in high case-fatality rates, alerting healthcare workers from HPS-endemic areas is warranted to detect co-infections in the context of the COVID-19 pandemic. In particular, at least 2 genotypes of Andes virus can be transmitted person-to-person, and these species are prevalent in 2 of the 3 hantavirus-endemic regions of Argentina (10) . In conclusion, we detected co-infection with SARS-CoV-2 and Andes virus causing HPS in a patient from a hantavirus-endemic area. Clinicians should be aware of the possibility of co-infection for patients originating, residing, or traveling in hantavirus-endemic areas. Computed tomography scan results on the second day of hospitalization (day 7 after fever onset) for a patient with severe acute respiratory syndrome coronavirus 2 and hantavirus coinfection, Argentina, 2020, showing pleural effusion, interstitial compromise, vascular congestion, and glass-ground opacities. Miss Coelho works in the Virology Department, Instituto Malbran, Buenos Aires, Argentina. Her primary research interests include epidemiology of infectious diseases and the diagnosis of respiratory viruses. Dr. Periolo works in the Virology Department, Instituto Malbran, Buenos Aires, Argentina. Her primary research interests include the virology of infectious diseases, immunology, and infectious respiratory diseases. Person-to-person transmission of Andes virus in hantavirus pulmonary syndrome Super-spreaders" and person-to-person transmission of Andes virus in Argentina Hantavirus pulmonary syndrome, southern Chile Boletín integrado de vigilancia N525 SE49 Hantavirus pulmonary syndrome in Argentina Hantavirus pulmonary syndrome Treatment of hantavirus pulmonary syndrome Epidemiological description, case-fatality rate, and trends of hantavirus pulmonary syndrome: 9 years of surveillance in Argentina Genetic diversity, distribution, and serological features of hantavirus infection in five countries in South America Person-to-person transmission of Andes virus Early Circulation of SARS-CoV-2 Niama Author affiliations: Laboratoire National de Santé Publique A fter coronavirus disease (COVID-19) was reported in China in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly spread around the world; most countries officially reported their first cases within the first 3 months of 2020. However, reports from China show a possible earlier first case on November 17, 2019, detected retrospectively in Hubei Province (1). Furthermore, phylogenetic analysis places the date of emergence as sometime during October-December 2019 (2) . These data suggest possible virus spread outside China before the first officially reported case in December 2019. Indeed, several retrospective studies that analyzed stored respiratory samples and wastewater for RNA detection, as well as serologic studies, suggest that SARS-CoV-2 may have been circulating in France, Spain, and Italy (3-7) before December 2019, months before the first official cases were reported.In central Africa, the first cases were officially reported during March 6-April 6, 2020; in Congo, the first case was reported on March 14, 2020. However, a 1 These first authors contributed equally to this article.To determine when severe acute respiratory syndrome coronavirus 2 arrived in Congo, we retrospectively antibody tested 937 blood samples collected during September 2019-February 2020. Seropositivity significantly increased from 1% in December 2019 to 5.3% in February 2020, before the first officially reported case in March 2020, suggesting unexpected early virus circulation.