key: cord-0982003-683iav3q authors: Shahid, Saba; Raza, Mohammad; Junejo, Samina; Maqsood, Sidra title: Clinical features and outcome of COVID‐19 positive children from a tertiary healthcare hospital in Karachi date: 2021-07-14 journal: J Med Virol DOI: 10.1002/jmv.27178 sha: c0732d90dbaab7b177cabc17d3d8fe37c6627964 doc_id: 982003 cord_uid: 683iav3q As the coronavirus disease 2019 (COVID‐19) pandemic continues to evolve, differences in epidemiological and clinical features among pediatrics have been noticed across different countries. We describe the spectrum of COVID‐19 in pediatric patients treated in tertiary health care. We conducted a retrospective chart review of pediatric patients admitted to Indus Hospital & Health care network, Korangi campus, Karachi; from April 1st, 2020 to July 31st, 2020. A total of 141 COVID‐19 cases were reported, males were 81 (57%) and the median age was 8 (0.3–17) years. Moderate and severe infections were noted in 36(26%), and 17(12%) children respectively. Fever (50%) was the most common clinical feature. The SF ratio less than 264 was significantly associated with severe disease (p < .05). Lab investigations that differed significantly across disease severity groups included IL‐6 levels (p < .01) and Prothrombin time (p < .05). Majority of children were advised home isolation 89 (63%), 29 (20.5%) were admitted while mortality was observed in 10 (7%) children. No significant difference was observed between children with and without malignancy. Pre‐existing comorbidities are significantly associated with COVID‐19 infections among children. Reduced SF ratio, elevated Prothrombin time, and interleukin‐6 levels are associated with greater disease severity. Although most COVID-19 infections in children are mild recently, there have been reports of severe COVID-19 infections in children known as Multisystem inflammatory syndrome (MIS-C). This syndrome shares common features with other pediatric inflammatory conditions, including Kawasaki Disease, and staphylococcal and streptococcal toxic shock syndromes. 10 MIS-C cases have been reported from Europe, 10 Italy, 11 and recently from Pakistan. 3 Most of the local literature on COVID-19 is based on the adult population, and gaps exist regarding clinical manifestations, lab investigations, and MIS-C management in children in Pakistan. It may not be prudent to follow adult guidelines for the management of COVID-19, as many infectious diseases affect children differently from adults. The current study is done to identify gaps in knowledge for pediatric covid infection in our population. Therefore, the current study is undertaken to describe epidemiological and clinical features, imaging data, laboratory findings, response to treatments, and outcomes of pediatric patients with COVID-19 in Karachi and compare the severity of illness with laboratory investigation through a retrospective chart review. A retrospective study of pediatric patients admitted to Indus Hospital and Health care network, Korangi campus, Karachi from April 2020 to July 2020 with laboratory-confirmed COVID-19 infection. All children between the ages of one month and 18 years, with evidence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection by a polymerase chain reaction in nasal swabs, were included. We also included patients admitted for other reasons but tested positive for COVID-19 infection during screening. Children from the Pediatric ward, intensive care, day care, and oncology unit were included. As per institutional policy tele-clinics were conducted for children who were advised home isolation. The outcomes noted in tele-clinic were recorded in computerized hospital records. A predesigned questionnaire was used to review data obtained from computerized health records of the hospital. The questionnaire was pilot tested for accuracy before the collection of data. Information was obtained regarding age, gender, pre-existing comorbidities, like heart disease, diabetes, immune compromise, rheumatologic disorders, and malignancy. The mode of the presentation was noted, which included contact tracing or screening for admission or day care management. Clinical features were noted along with the clinical course in terms of the presence and nature of organ failure, respiratory support (like oxygen therapy, noninvasive ventilation, high-flow nasal cannula, or invasive ventilation) and additional organ support, like vasoactive medications and renal replacement therapy. Information was collected for lab investigations and imaging studies. Laboratory investigations included in the study consisted of complete blood count (absolute leukocyte count, absolute neutrophil count, neutrophil-lymphocyte ratio, and hemoglobin), serum biochemistry (C-reactive protein, Procalcitonin, lactate dehydrogenase, alanine aminotransferase, total bilirubin, creatinine, prothrombin time, partial thromboplastin time and D-dimer), interleukin-6 levels (IL-6), Troponin-I, B-type natriuretic peptide (Pro-BNP) levels, imaging studies (chest X-ray and computed tomography [CT] chest, CT brain), and echocardiography. Chest imaging (chest X-ray and CT scan chest) was categorized in typical, indeterminate, and atypical findings based on the expert consensus statement. 12 Pharmacotherapy details were noted, which included antibiotics, Steroids, Remdesivir, Tocilizumab, intravenous Immunoglobulin, and Aspirin. Absolute leukocyte count less than 1.5 × 10 9 /L and less than 1.0 × 10 9 /L were considered low for children less rhgan 6 years and between 6 and 18 years of age, respectively. ANC of less than 1.5 × 10 9 /L was considered low, and neutrophil to lymphocyte ratio greater than 3.1 was considered significant. COVID-19 infection was classified into mild, moderate, and severe categories based on institutional and WHO guidelines. 13 Clinical features, lab investigations, and chest imaging findings were used to categorize illness. The same classification was used to categorize disease severity in children with and without malignancy. The mild/asymptomatic category included children who had mild symptoms or were asymptomatic and identified during screening or contact tracing. Moderate category consisted of at least two-organ involvement, which included severe diarrhea, dehydration, pneumonia, motor weakness with or without loss of consciousness, hepatitis, presence of lab derangements or SpO2 > 90%. Severe disease included multiorgan dysfunction, Spo 2 < 90% on five liters of oxygen pediatric acute respiratory distress syndrome (PF ratio <300 or SF ratio <264), respiratory failure, shock, central nervous system involvement, cardiac manifestations, or child requiring assisted ventilation. MIS-C category with features of either Kawasaki or toxic shock syndrome was included in severe disease. Approval from the ethical review board of the hospital was taken before starting the study. The admitted patients' clinical outcomes were noted at discharge or death while for the nonadmitted patients, the outcome was noted from tele-clinic records. The study's main outcomes were clinical characteristics and trajectory of the disease and outcomes of children with SHAHID ET AL. | 5989 confirmed COVID-19 infection. Secondary outcomes included the association of clinical severity with laboratory investigations. SPSS version 20 was used to calculate the frequency of qualitative variables, that is, gender, mode of presentation, symptoms, medication history, chest imaging, echocardiographic features, treatment received, and outcome. The mean, median, and standard deviation, interquartile range, and confidence interval were calculated for quantitative variables, such as age, duration of hospital stay, SF ratio, hematological markers, biochemistry markers, inflammatory markers, and myocardial enzymes. The χ 2 test was applied to compare differences between categorical variables. An independent sample t test and one-way analysis of variance were calculated to find the mean differences. p < .05 was considered as significant. A total of 165 cases were included, out of these 24 children had unknown outcomes and were excluded. The remaining 141 children were included in the final analysis, out of which 80 (57%) had malignancies while 61 (43%) children had nonmalignant conditions ( Figure 1 and Table 1 ). The median age was 8 years, 3-12 males were 81 (57%) while females were 60 (43%). Contact tracing identified 42 (30%) children, 97 (69%) children tested positive on admission screening and 2 (1%) came positive during hospital stay ( Table 1 ). The maximum number of cases were seen in June (55% 78/141) followed by May (28%; 40/141). Asymptomatic children were 88 (62%), while moderate and severe disease were seen in 36 (26%) and 17(12%) children, respectively. Overall the most common presenting symptoms were fever 71/141 (50%), gastrointestinal complaints 36/141 (25%), and respiratory tract symptoms 32/141 (22%) ( Table 1) . Neurological complications were less commonly seen (7/141; 5%). Among seven children with neurological complications, seizures and neuromuscular weakness were the common symptoms (Table 1 ). Oxygen therapy was given to 16 (11%) children while 6 (4%) required invasive ventilation. The mean duration of oxygen therapy in the severe group was 6.3 ± 6.6 days. Inotropic support was given to 6/16 (37%) children in the severe group ( Thirteen (8%) and 10 (59%) children received injectable steroids while Remdesivir and IVIG were given to three children (Table 3 ). No difference was noted in clinical features and demographic features between the two groups. B-type natriuretic peptide (p = 0.040) and D-dimer (p = 0.07) were statistically significant between the two groups ( Table 4 ). We observed a median age of 8 (0.3-17) years, which is consistent with other studies that report the ages ranging from 6 to 11 years. 14 Slight male preponderance was noted in our cohort, 81 (57%). World-wide data reports the mixed-gender distribution of COVID- 19, 15, 16 which suggests that regional variations of demographic variables may exist among children with COVID-19. The clinical course of the disease was mild in most of the children. The majority of the children 88/141 (62%) were asymptomatic or had mild clinical manifestations. Similar findings have been reported in other parts of the world. 4, 8, 14 There is evidence that ACE-2 proteins serve as receptors for both SARS-CoV and SARS-CoV-2. These proteins are immature in children, thereby allowing less virus adherence and milder infection in children. 17 Presence of a robust immunity in children also protects them from viral replication 18 Cardiac involvement has also been reported in a cohort of European children. 24 Evidence shows that hypoxia and electrolyte imbalance in the acute phase of the disease is linked to cardiac arrhythmias, 25 while raised inflammatory markers like IL-1β, IFN-γ, and IL-6 are associated with myocardial injury. 24 These factors may have caused impaired cardiac function in our children. We found a significant association of raised Prothrombin time and D-dimer with disease severity, which has been observed in other children with severe COVID-19. 26 adults. 4 On the contrary, our findings showed lymphocytosis in severe disease. Kainth et al. 27 also reported lymphocytosis in a cohort of 65 children in the United State. 28 These differences in hematological parameters suggest that it may not be prudent to draw parallels between adults and children in terms of complete blood count. Instead, leukocytosis and lymphocytosis in pediatric COVID-19 infections should alert physicians for the possibility of severe disease, and may be used as a prognostic marker. The participants of our study were children admitted to the hospital. Therefore, our results are not representative of children with COVID-19 in ambulatory settings. The study site was limited to a single tertiary care hospital and the results may not be generalizable to the whole population. This study adds valuable information to the body of literature on COVID-19 infections in children. Males are more prone to higher mortality compared to females. There was no significant difference among children with or without malignancy. Elevated Prothrombin time, D-dimer, B-type natriuretic peptide, and IL-6 levels are shown to be associated with disease severity. They may be studied further for consideration as prognostic markers in children with severe COVID-19 disease. 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The authors declare that there are no conflict of interests. The research data are confidential.