key: cord-1008354-qz6y4ozo authors: Krueger, Mariana Braatz; Montenegro, Raquel Carvalho; de Araújo Coimbra, Pablo Picasso; de Queiroz Lemos, Luanna; Fiorenza, Regiane Martins; da Silva Fernandes, Carla Jéssica; Pessoa, Mariana Santos Leite; Rodrigues, Cleonísio Leite; da Cruz, Camilla Gomes; de Araújo Verdiano, Verlene; de Carvalho Araújo, Fernanda Montenegro; Braga-Neto, Pedro; Sobreira-Neto, Manoel Alves title: A wide spectrum of neurological manifestations in pediatrics patients with the COVID-19 infection: a case series date: 2021-08-26 journal: J Neurovirol DOI: 10.1007/s13365-021-01004-9 sha: 939c646aa25eac8d07b319a8e18eae756c83cff8 doc_id: 1008354 cord_uid: qz6y4ozo Neurological symptoms in COVID-19 patients can also be found in the pediatric population, but they are usually described as mild symptoms. Herein, we described a case series of four pediatric patients with severe and highly heterogeneous central and peripheral nervous system manifestations. The objective was to report neurological manifestations of COVID-19 in children and adolescents. The design is case series. The participants are four children and adolescents with confirmed COVID-19. The main outcome and measures are as follows: Clinical data were gathered from electronic medical records, and data of all neurologic symptoms were checked by a trained neurologist. We reported four pediatric patients with COVID-19 and different neurologic symptoms. Case 1 was a 16-year-old girl with a sensory and motor polyradiculopathy with RT-qPCR for COVID-19 and dengue both detected in CSF that improved after appropriate treatment. Case 2 was a 15-year-old boy with Guillain–Barre syndrome and had good response after using human immunoglobulin. Case 3 was a 5-year-old girl with acute intracranial hypertension that improved after going through lumbar puncture and using acetazolamide. Case 4 was a 2-month-old male infant with focal epileptic seizures that recovered after antiepileptic treatment. We highlight the need to consider different neurologic manifestations as part of the COVID-19 clinical spectrum. in the pediatric population it is commonly described as a disease with mild symptoms (She et al. 2020; Dong et al. 2020) . In children, fever and cough are the most common clinical manifestations, which sometimes is combined with fatigue, myalgia, nasal congestion, runny nose, sneezing, sore throat, vomiting and abdominal pain (She et al. 2020) . The most common neurological manifestations in adults infected by COVID-19 infection in adults are dizziness, headache, anosmia, stroke, ataxia, and epilepsy (Abdel-Mannan et al. 2020; Mao et al. 2019) . Neurological symptoms could also be found in the pediatric population, such as headache, muscle weakness, and encephalopathy (She et al. 2020; Dong et al. 2020; Abdel-Mannan et al. 2020 ). Herein, we described a case series of four pediatric patients with COVID-19, from a pediatric hospital at Fortaleza, There are no prior publications or submissions with any overlapping information, including studies and patients. A 16-year-old girl with diarrhea presented, after 5 days, paresthesia and progressive difficulty to walk. Neurological examination showed acute and progressive paraparesis, with hypoesthesia below Th6 level. No sphincter dysfunction was reported. Brain, cervical, thoracic, and lumbar MRI showed contrast enhancement in the anterior roots of the medullary cone, as well as in the bilateral cranial nerves VII/VIII complex ( Fig. 1 ). Cerebrospinal fluid (CSF) analysis showed pleocytosis with predominance of lymphocytes (Table 1 ). Real-time qPCRs for SARS-CoV-2 (RT-qPCR-COVID) and dengue virus, both in CSF, were positive. COVID-19 serological rapid test IgM/IgG (COVID-RT) was also positive. Serial electroneuromyography (EMG) assessment showed normal nerve conduction studies (NCS) associated with absence of H reflex, widespread fibrillations, and positive waves (including in paraspinal muscles) associated with acute neurogenic motor recruitment, compatible with polyradiculopathy. The patient was treated at day 9 with acyclovir, for 14 days, followed by intravenous human immunoglobulin 0.4 g/kg/day, for 5 days. At day 17, we used methylprednisolone 1 g for 5 days. She presented with progressive A 15-year-old boy presented, initially, rhinorrhea and dry cough. The disease evolved after 15 days with pain, paresthesia, and weakness in the lower limbs followed by the upper limb's involvement, without respiratory impairment. No bladder or bowel dysfunctions were reported. Neurological examination showed absence of deep tendon reflexes, quadriparesis, and reduction of superficial and deep sensitivity at the four limbs. COVID-RT was positive. CSF analysis showed mild pleocytosis with increased protein (Table 1 ). Brain MRI was normal, but thoracic and lumbosacral MRI showed enhancement of the nerve roots, mainly at ventral roots (Fig. 1) . Electrophysiological examination showed demyelination features in peripheral nerves with prolonged distal motor latencies, non-uniform decrease of motor nerve conduction velocities, and prolonged F wave latencies with no active denervation in needle EMG. The patient received 0.4 g/kg/day of intravenous human immunoglobulin for 5 days with a significant improvement, restoring the ability to walk independently after 2 weeks. A 5-year-old girl, with previous hypertensive arachnoid cyst treated with ventriculoperitoneal derivation, was asymptomatic since surgery. She was admitted with intense headache, fever, vomiting, and horizontal diplopia. Neurological examination confirmed the horizontal diplopia, with no other findings. Brain MRI and digital angiograph were normal (Fig. 2) . CSF showed an open pressure of 70 cmH 2 O (normal < 25cmH 2 O) and normal analysis (Table 1) . COVID-RT was positive. The symptoms improved after CSF withdrawal and the use of acetazolamide, with gradual reduction after 1 month. A 2-month-old male infant, with a term deliver without any problem, presented dry cough, fever, and diarrhea. After 15 days, these symptoms were followed by dyspnea and hypoxemia, needing mechanical ventilation. His mother was diagnosed with COVID-19, and the COVID-RT of the patient was also positive. After 3 days of the admission, he presented with the deviation of the eyes and automatic masticatory movements. Brain MRI, angiography MRI, and CSF analysis were normal (Table 1) . Electroencephalography with synchronized video showed acute transients and brief ictal-appearing rhythmic discharges (BIRDs) at posterior regions (bilateral temporo-occipital region) (Fig. 2) . He was treated with intravenous phenobarbital with an improvement of the epileptic events and was discharged after 25 days without any apparent neurologic deficits. After 3 months, the patient was reevaluated, without new epileptic seizures and with normal neurological examination. Herein, we described four pediatric patient cases with different neurological manifestations associated with COVID-19, involving both the central and peripheral nervous systems, and their responses to different treatments. The first report of Guillain-Barré Syndrome (GBS) associated with COVID-19 occurred with a 61-year-old woman in the end of January 2020 (Zhao et al. 2020) . From that date up to now, other cases were described, but neither of them in pediatric patient (Badat et al. 2018; Li et al. 2020) . Possible mechanism for this coexistence is a molecular mimicry between the new coronavirus and the peripheral nerve, creating an immune-mediated process (Finsterer et al. 2020) . The virus was found in CSF of patient 1 together with dengue virus, an endemic condition in our region. This coinfection could exacerbate the immune response, but we should also consider direct virus lesion. As documented in the previous papers, the onset of the neuropathy symptoms of patient 1 and 2 overlapped with the period of the infection (Li et al. 2020; Toscano et al. 2020 ). This pattern is distinct from the classic postinfectious profile, reported in GBS. A recent case of idiopathic intracranial hypertension (IHH) was described in a 35-year-old woman with COVID-19, and, as patient 3, she also had an acute increase of intracranial hypertension (Noro et al. 2020) . Possibly, the pathological process of the disease interfered with cerebrospinal liquid dynamic, but the mechanism is not clear. Recently, a large population-based case-control study demonstrated an association between infectious and inflammatory disorders with the IHH (Sundholm et al. 2020) . The epileptic seizure is a common symptom in COVID-19 patients, and recently, a study using electroencephalography in COVID-19 critically ill patients documented seizure in 63.6% of them (Galanopoulou et al. 2020 ). This study reported epileptiform activity more often in the frontal region, but patient 4 presented the epileptiform activity at the brain's posterior regions. The reason for the seizures in our patient is probably related to hypoxia and its consequences. However, we could not preclude the virus invasion and the immune attack to the central nervous system (Zhao et al. 2020) . A causal relationship between the virus and the different neurologic disorders in children should be confirmed with a larger population and a longitudinal study. Although these manifestations are rare in this population, the cases reported here reinforce the need to evaluate them in COVID-19 pediatric patients. Neurologic and radiographic findings associated with COVID-19 infection in children Risk of transverse myelitis following dengue infection: a systematic review of the literature Epidemiology of COVID-19 among children in China COVID-19 polyradiculitis in 24 patients without SARS-CoV-2 in the cerebro-spinal fluid [published online ahead of print EEG findings in acutely ill patients investigated for SARS-CoV-2/COVID-19: a small case series preliminary report Involvement of the nervous system in SARS-CoV-2 infection Neurologic manifestations of hospitalized patients with coronavirus Disease COVID-19 and benign intracranial hypertension: a case report COVID-19 epidemic: disease characteristics in children Nilsson Remahl AIM (2020) Infectious and inflammatory disorders might increase the risk of developing idiopathic intracranial hypertension -a national case-control study Cephalalgia 333102420928079 Guillain-Barré Syndrome associated with SARS-CoV-2 Guillain-Barré syndrome associated with SARS-CoV-2 infection: causality or coincidence?