key: cord-1008831-4y3x7ijd authors: Echternach, Matthias; Gantner, Sophia; Peters, Gregor; Westphalen, Caroline; Benthaus, Tobias; Jakubaß, Bernhard; Kuranova, Liudmila; Döllinger, Michael; Kniesburges, Stefan title: Impulse Dispersion of Aerosols during Singing and Speaking: A Potential COVID-19 Transmission Pathway date: 2020-12-01 journal: Am J Respir Crit Care Med DOI: 10.1164/rccm.202009-3438le sha: 03c8abcf31d365713bd4c40cc4956d017df9629c doc_id: 1008831 cord_uid: 4y3x7ijd nan Three full HD Sony HDC 1700R cameras recorded the experiment from side (camera 1) and top view (camera 2) perspectives. All measurements were performed in a Bavarian Broadcasting television network studio (dimensions, 27 m 3 22 m 3 9 m). The walls were at least 4 m away and covered in black. The smoke was illuminated with three spotlights positioned in a distance of at least 3.5 m. Before each task, the studio was aired out. After aeration, all people present were instructed not to move for another 2 minutes. The temperature was measured at mean (SD) 23.27 8 C (0.46) and the relative humidity at 46.12% (0.95). The cloud of smoke was segmented in each video frame using a threshold-based region-growing algorithm yielding the area of the cloud and its contour as a function of time in three dimensions from the mouth of the singer (x-dimension to the front, y-dimension from the left to the right, and z-dimension from the bottom to the top). The dimensions of the region of interest (ROI) were ROI x 3 ROI y 3 ROI z = 260 cm 3 270 cm 3 180 cm for camera 1 and 190 cm 3 270 cm 3 180 cm for camera 2. The impulse dispersion in x-direction was found to be greater than in y-or z-direction. The median distance to the front was 0.86 m for MT1, 0.78 m for MT2, 0.82 m for T1, and 0.74 m for T2 at the end of the tasks. The M tasks revealed distinctly lower values with 0.62 m (M1) and 0.49 m (M2), respectively (Friedmann/Wilcoxon/Bonferroni-correction P values: MT6 vs. M6 = 0.003, T6 vs. M6 = 0.015, and MT6 vs. T6 = nonsignificant). The intersubject variability was large, ranging from 0.61 m to 1.36 m for the MT tasks ( Figure 1 ). Once a task was completed, the motion of the aerosol cloud decreased with an additional median movement to the front (x-direction) between 0.04 m and 0.11 m for all tasks 3 seconds after the end of task. The dispersion to the side was much less ( Figure 2 ). However, the distances in y-direction show a lateralization imbalance for some subjects, presumably because of a small convectional flow generated by the singer's motion immediately before the beginning of the task. The y-diameter between left and right exhibited median values between 0.57 m and 0.88 m at the end of the task. The With regard to both breathing and coughing tasks, detected distances were much greater than all phonation related tasks. After 6 seconds of exhalation, the median distance in the x-direction was 1.19 m (maximum 1.71 m), and after coughing, the median distance in the x-direction was 1.32 m (maximum 1.89 m). Although the median distance to the front reached values ,1 m for the MT1 and MT2 tasks, many subjects reached greater distances of up to 1.4 m. The dispersion distance to the side was much lower. Because of the maximum dispersion, no distances lower than 2-2.5 m between persons to the front and 1.5 m to the side should be recommended as safety distance. However, safety is not only dependent on the measured near field under controlled laboratory conditions but also on the accumulation of aerosols over time during phonation and the convectional flow in realistic environments. Therefore, a continuous ventilation and/or filtration of the air volume during singing could diminish the amount of aerosols and therefore reduce the risk of infection transmissions. Furthermore, wearing masks could affect the speed of aerosol dispersion; however, it could also restrain the articulation. The softer tasks showed a tendentially lower dispersion to the front than the louder tasks. Loudness is dependent on the transglottical pressure difference (3), which generates greater airflow. In agreement with previous studies (4, 5) , the largest frontal dispersion was found for coughing. Furthermore, Asadi and colleagues found that the absolute aerosol production was greater for louder phonation (6) . As a consequence, the potential transmission risk appears increased for loud phonation, resulting from both the absolute aerosol production and the tendentially greater dispersion distance to the front. The generalization of this study is limited by its inclusion of only professional singers and a consequently low number of subjects. Also, the gas from the e-cigarette might have influenced the singing. Lastly, the study used an artificially added aerosol with a comparable amount of aerosols for each task. The real number of aerosols expelled during phonation is, however, much lower. It has been found that for voiced counting, the number of expelled droplets with sizes of 0.3-20 mm was 0.322 cm 23 and was approximately three times higher during singing (7) . n To the Editor: Mutations in the BMPR2 (bone morphogenetic protein receptor type 2) gene account for most heritable pulmonary arterial hypertension (PAH) cases. However, pathologic expression only occurs in approximately 42% of female, and 14% of male, carriers, with onset of BMPR2-associated PAH (BMPR21PAH) varying across the lifespan (1). Limited data exist regarding the onset of pulmonary artery pressure (PAP) elevation, etiologies for phenotypic expression, and the optimal frequency of screening. We report two cases that highlight challenges for adolescents and young adults at risk of BMPR21PAH. A healthy 17-year-old female, whose case timeline is detailed in Figure 1 , was evaluated because of a family history of BMPR21PAH. At initial evaluation, she had no signs or symptoms of PAH, a normal physical exam, and a history of regular exercise without exertional symptoms. Her echocardiogram demonstrated normal biventricular function and no evidence of right ventricular pressure (RVP) elevation. Six months later, echocardiography demonstrated normal RVP estimate and normal biventricular function. Fifteen months following catheterization, and 9 months following her last normal echocardiogram, she presented with a syncopal episode and increasing dyspnea over the preceding 6 weeks after starting college. Echocardiography showed mild right ventricular dilation with elevated RVP. Her laboratory evaluation was remarkable only for a slight elevation in white blood cell count of unclear significance. Evaluation for pulmonary embolism with D-dimer, _ V/ _ Q scan, and single-photon emission computed tomographic/computed tomographic scan was negative. Of note, she had an etonogestrel/ ethinyl estradiol vaginal ring in place for birth control, which had been used since before her genetic testing with negative pregnancy tests. She denied illicit drug use. Catheterization revealed a CI of 3.9 L/min/m 2 , mean PAP of 52 mm Hg, and PVRI of 5.1 WU, unresponsive to acute vasodilator treatment. Sildenafil and ambrisentan were initiated. Repeat catheterization 6 months later demonstrated a CI of 3.5 L/min/m 2 , PAP of 40 mm Hg, and PVRI of 4.8 WU. Selexipag was added. A healthy 16-year-old male presented for evaluation because of a family history of BMPR21PAH associated with a heterozygous missense mutation in BMPR2 (c.354T.G [p.Cys118Trp]). Of note, he had an echocardiogram at age 12 for murmur, which showed normal anatomy and function. At initial evaluation, he reported a history of albuterol-responsive wheezing, occasional chest pains but none in over a year, and an admitted lack of interest in physical fitness. His exam was notable for a body mass index of 35.3 kg/m 2 (.97th percentile) and a pronounced second heart sound. His echocardiogram demonstrated normal heart structure, normal biventricular function, mild septal flattening, and elevated tricuspid regurgitant velocity gradient suggestive of RVP elevation (z50 mm Hg). Routine fasting bloodwork for new PAH diagnoses (including HIV, C-reactive protein, and complement levels) were normal except for a blood glucose of 115 mg/dl consistent with a diagnosis of prediabetes. Cardiac catheterization revealed a CI of 2.5 L/min/m 2 , mean PAP of 38 mm Hg, and PVRI of 10.9 WU, unresponsive to acute vasodilator testing. He was started on sildenafil. Comprehensive workup confirmed the family's BMPR2 gene mutation. At All authors have made substantial contributions to the conception of the work, have revised the work critically for important intellectual content, have approved the final version to be published, and agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. This letter has a related editorial. Originally Published in Press as DOI: 10.1164/rccm.202005-1611LE on July 21, 2020 High SARS-CoV-2 attack rate following exposure at a choir practice -Skagit County Electronic cigarette aerosol particle size distribution measurements The science of the singing voice Turbulent gas clouds and respiratory pathogen emissions: potential implications for reducing transmission of COVID-19 Epidemiology of primary tuberculosis in an industrial school Aerosol emission and superemission during human speech increase with voice loudness Size distribution and sites of origin of droplets expelled from the human respiratory tract during expiratory activities