key: cord-1009610-z1z9wd9e
authors: Noh, Inhwan; Lee, Kyuri; Rhee, Yun-Seok
title: Microneedle systems for delivering nucleic acid drugs
date: 2022-01-04
journal: J Pharm Investig
DOI: 10.1007/s40005-021-00558-4
sha: ea3cd6d09e757821ac059c411ea192cbdca69e9e
doc_id: 1009610
cord_uid: z1z9wd9e

BACKGROUND: Nucleic acid-based gene therapy is a promising technology that has been used in various applications such as novel vaccination platforms for infectious/cancer diseases and cellular reprogramming because of its fast, specific, and effective properties. Despite its potential, the parenteral nucleic acid drug formulation exhibits instability and low efficacy due to various barriers, such as stability concerns related to its liquid state formulation, skin barriers, and endogenous nuclease degradation. As promising alternatives, many attempts have been made to perform nucleic acid delivery using a microneedle system. With its minimal invasiveness, microneedle can deliver nucleic acid drugs with enhanced efficacy and improved stability. AREA COVERED: This review describes nucleic acid medicines' current state and features and their delivery systems utilizing non-viral vectors and physical delivery systems. In addition, different types of microneedle delivery systems and their properties are briefly reviewed. Furthermore, recent advances of microneedle-based nucleic acid drugs, including featured vaccination applications, are described. EXPERT OPINION: Nucleic acid drugs have shown significant potential beyond the limitation of conventional small molecules, and the current COVID-19 pandemic highlights the importance of nucleic acid therapies as a novel vaccination platform. Microneedle-mediated nucleic acid drug delivery is a potential platform for less invasive nucleic acid drug delivery. Microneedle system can show enhanced efficacy, stability, and improved patient convenience through self-administration with less pain.

Gene therapy is a promising technology for treating lifethreatening diseases by engineering a specific gene target (Mulligan 1993) . In response to the recent COVID-19 pandemic, DNA and mRNA vaccines have been shown the utility of nucleic acid-based vaccines with a rapid response to emerging infectious diseases as safe and useful treatment options (Haynes 2021; Dagan et al. 2021 ). In addition, nucleic acid drug has been demonstrated to have a great potential for decades as a cancer vaccine that may not be achievable with conventional vaccine approaches (including previous small molecules and protein-based therapeutics approach or tools) for cellular reprogramming or trans-differentiation (Kwon et al. 2018; Pardi et al. 2018; Hu et al. 2020; Morshed et al. 2020) .

Despite its potential, delivering a nucleic acid to target sites such as the nucleus and the cytoplasm inside the cell of target tissues faces several hurdles due to its charged and macromolecular nature (McCaffrey et al. 2015; Hu et al. 2020) . First, an exogeneous nucleic acid medication must pass through the skin's tight junctions and then the cell membrane or nucleus. Then it needs to resist degradation by endogenous enzymes such liver metabolism and nucleases until its internalization into the cell (Sorrentino 2010) . As a result, it relies on a drug delivery system that can protect against nuclease and protein binding while allowing for enhanced membrane penetration (McCaffrey et al. 2015; Pardi et al. 2018; Hu et al. 2020) . Many previous studies on nucleic acid medicines have employed intramuscular or subcutaneous injection as a parenteral method coupled with nanocarriers (Pardi et al. 2018; Hu et al. 2020) . However, these parenteral injection routes might be unpleasant (I-Hong Hsu 2016 ). In addition, they need medical personnel to perform the injection. Furthermore, nucleic acid medicines are less stable since the medication must be reconstituted in a liquid form prior to injection, which may present distribution, storage, and shelf-life concerns. Significant stability issues of mRNA vaccines for COVID have recently been addressed in this respect (Schoenmaker et al. 2021) .

Microneedle (MN) can be used as one promising approach for nucleic acid delivery. As one of transdermal drug delivery systems, MN can create physical channels for drugs to bypass the stratum corneum layer, which serves as a strong barrier for transdermal administration. With a minimally invasive manner, MN system allows nucleic acid penetration and self-application. It also improves patient compliance by enhancing user convenience due to its simple application technique with reduced discomfort compared to parenteral preparations. It also has the benefit of improved stability of drugs because drugs are encapsulated in a dried state (Pearton et al. 2012; Liao et al. 2017; Schoenmaker et al. 2021) .

This review examines the current state and characteristics of nucleic acid drugs and delivery systems, as well as a brief overview of the various types and characteristics of microneedle systems. Recent advancements in MN-based nucleic acid drugs for vaccination against infectious diseases or cancer are also discussed. Furthermore, research on microneedle formulation and morphology, as well as combination approaches of microneedles with other delivery systems, is reviewed as strategies to improve the transfection efficacy of microneedle-based nucleic acids. Finally, limitations and factors to consider when developing microneedles for nucleic acid delivery are discussed.

Plasmid is a circular double-stranded DNA molecule that originates from the cytoplasm of bacteria (Wolff et al. 1990 ). It can be applied to gene therapy by expressing target protein in cells. It has the advantages of easy handling (such as storage and transport due to its high stability) and easy mass production. As a DNA vaccine, it can be used for treating infectious disease or cancer by encoding tumor-associated antigen (Lopes et al. 2019; Eusébio et al. 2021) . Plasmid DNA has been studied in various applications such as regenerative medicine using induced pluripotent stem cells (iPSCs) by transfecting plasmid DNA encoding reprogramming factors like Yamanaka factor (Karow et al. 2011 ).

However, because plasmid DNA may contain bacteriaderived foreign genes, it may induce adverse effects such as inflammation (Yew et al. 2000) . Furthermore, it faces an issue of poor transfection efficiency that needs to be addressed. Researchers have utilized viral vectors, non-viral vectors, and physical delivery system to improve its transfection efficiency .

In the case of using a viral vector, it may show a relatively high expression level. However, it has limited payload due to the size of the viral vector with a poor productivity (McClements and Maclaren 2017) . Additionally, immunogenicity of viral vector itself limits repeated administration such as boost inoculations (Hardee et al. 2017) . More critically, the viral-vector delivery system has shown significant risks of potential insertion mutation and carcinogenicity. As an alternative, a non-viral vector using cationic polymer or lipid nanoparticles can alleviate immunogenicity and decrease safety concerns associated with a viral vector (Schleef 2013; Hardee et al. 2017) . However, it also has the issue of low expression efficiency that needs to be addressed. Recent achievements in non-viral vectors and physical delivery systems have enhanced the transfection efficiency and reduced the toxicity (Hardee et al. 2017; Kulkarni et al. 2018). siRNA In 1998, Fire et al. discovered that RNA interference (RNAi) could be utilized for gene therapy (Fire et al. 1998) . siRNA can mediate target mRNA degradation by forming RNA-molecules induced Silencing Complex (RISC) in a sequence-specific manner, followed by knock-down expression of a target gene encoding a protein (Setten et al. 2019; Hu et al. 2020 ). Unlike monoclonal antibodies or small molecules medicines designed to recognize complex structures of target proteins using screening technologies such as structure activity relationship (SAR), siRNA can inhibit a specific mRNA with a sequence-specific manner, allowing researcher to target specific genes of interest (Zhang et al. 2021) . Therefore, siRNA can be applied to treat a wide spectrum of diseases with a comparatively short development period (Hu et al. 2020) .

However, in the early stage of research on siRNA-based therapies, systematically administered siRNA without a carrier (naked) was rapidly degraded by nuclease and rigorously excreted owing to its small size, resulting in poor pharmacokinetics and low efficacy (Huang et al. 2011) . siRNA shows immunostimulatory properties by binding to pattern recognition receptors (PRRs) such as TLR3 at several locations (for example surface of target cell, endosomal, or cytosol level), which induces adverse effects such as inflammation (Cho et al. 2009 ). Furthermore, siRNA may face difficulties of escaping from the endosome or lysosome to the cytoplasm after siRNA enter the cell, it may show a low efficiency Hu et al. 2020) .

These difficulties can be alleviated by the development of chemical modification and delivery systems such as nanocarriers, which can protect systemically administered siRNA from various degrading enzymes or serum protein binding, thus greatly improving its stability and pharmacokinetic behaviors (Khvorova and Watts 2017; Setten et al. 2019; Hu et al. 2020) . These developments have also increased siRNA intracellular permeability with improved efficacy. As a result, various therapeutics using advanced siRNA system have been reported, including cancer therapy. ONPATTRO® (Patisiran), the first authorized siRNA medicine, was successfully introduced in 2018, demonstrating the promise of siRNA treatment, followed by GIVLAARI® (Givosiran) in 2019 and OXLUMO® (Lumasiran) in 2020, all of which were successfully commercialized. Recently, the European Commission (EC) approved LEQVIO® (inclisiran) for the treatment of adults with hypercholesterolemia or mixed dyslipidemia. In September 2021, LEQVIO® received NICE (National Institute for Health and Care Excellence) approval for use by the National Health Service in the UK. Most of siRNA medicines including many other siRNA candidates in clinical trials are targeting rare diseases or diseases that urgently need novel and effective therapies (Zhang et al. 2021 ). mRNA mRNA is a single-stranded RNA that encodes proteins. It usually has a rapid onset and better protein expression than plasmid DNA because protein expression from mRNA through translation is feasible at the cytoplasm level, as opposed to pDNA which must enter the nucleus (Kwon et al. 2018; Liu 2019) . In addition, it is safe since mRNA poses no potential risk of insertional mutagenesis or carcinogenesis shown in plasmid DNA treatment. mRNA is finally degraded by endogenous enzymes inside or outside of cells.

However, like an siRNA, the intrinsic poor stability of mRNA limits its applications in its early stage of development (Kwon et al. 2018) . mRNA can also induce immune responses due to its innate immunostimulatory properties by stimulating several pattern recognition receptors (PRRs) such as TLR7 and TLR8 on the cell surface and endosomal levels (Heil 2004; Pardi et al. 2018 ). These immunological properties can also lead to decreased expression levels of mRNA caused by type-1 interferon secretion (Pardi et al. 2018) . Furthermore, an efficient synthesis method of mRNA is lacking. As a result, mRNA has not received much attention compared to other nucleic acid drugs in the early stage of gene therapy development.

In the 1990s, along with the development of a simple and efficient method for in vitro transcription (IVT) of mRNA (Wolff et al. 1990; Sahin et al. 2014) , the stability and efficacy of mRNA were much improved by introducing chemically modified nucleosides or nanocarrier complexes, similar to siRNA (Karikó et al. 2008; Kwon et al. 2018 ). Furthermore, expression efficiency could be greatly increased by sequence optimization each structure of mRNA (for example, 3' and 5' untranslated regions and poly A tail) (Kwon et al. 2018 ) and minimizing immune stimulation caused by dsRNA contamination through additional purification such as size exclusive chromatography (Karikó et al. 2011) . In recent years, these advances have made mRNA a promising alternative to plasmid DNA. Through global inoculation, the safety and efficacy of mRNA vaccines have been considerably established (Polack et al. 2020) . Not only mRNA vaccines for infectious diseases, but also mRNA technologies can be applied as regenerative medicine via induced pluripotent stem cells or cancer vaccines using tumor-associated antigen encoding mRNA in a sequence-specific manner, showing significant potential and feasibility of gene therapy (Kwon et al. 2018; Pardi et al. 2018 ).

To be effective, a nucleic acid drug should enter the body via a parenteral route and permeate the lipid membrane barrier of the cell so that it could be delivered to its site of action such as the cytoplasm or nucleus (McCaffrey et al. 2015; Hu et al. 2020) . Although researchers have discovered several types of cells that can absorb nucleic acids, permeation of nucleic acid into cells is regarded a difficult process for most cells due to characteristics of nucleic acid such as a large size and surface charge. Therefore, a well-designed delivery system for nucleic acid to reach the target cell is a critical element that affects its clinical efficacy (McCaffrey et al. 2015; Pardi et al. 2018; Hu et al. 2020 ).

A naked nucleic acid delivery system refers to a system in which a nucleic acid drug has no covalent or non-covalent association with the delivery system. Because it is directly exposed to the environment it makes contact with, naked nucleic acid cannot be protected from various degrading enzyme or binding proteins. Thus, it can be metabolized or excreted rapidly (Huang et al. 2011; Hu et al. 2020) . It is also difficult for a naked nucleic acid to permeate the cell membrane due to its negative charge and large size. Even when nucleic acid is introduced into cells, it is usually difficult to escape from the endosome or lysosome Patel et al. 2019 ). In addition, as mentioned above, nucleic acids can activate the immune system by stimulating various PRRs at the surface, cytosol, or endosome levels, inducing interferon secretion, thereby causing inflammation or reducing protein expression efficiency. Therefore, to utilize naked nucleic acid, it is necessary to use chemical modified nucleosides to prevent degradation or reduce immune stimulation (Hu et al. 2020 ).

On the other hand, facilitated excretion of naked nucleic acid drugs by kidney allows chemically modified nucleic acids to target renal diseases (Huang et al. 2011; Hu et al. 2020) . Rather than systemic injection, naked nucleic acid drugs can be administered directly into specific organs or tissues such as the eye with comparatively low excretion or low nuclease levels (Hu et al. 2020 ). In addition, immune stimulating properties by activating PRRs can be an effective strategy to deliver nucleic acid vaccine (Pardi et al. 2018; Hu et al. 2020 ).

Non-viral vectors for the delivery of nucleic acid drugs are expected to increase efficacy of nucleic acid drugs by preventing them from degradation and renal excretion, thus facilitating transcellular delivery by endocytosis with reduced charged nature of the complex or inhibiting their interactions with non-specific plasma proteins (Ramamoorth and Narvekar 2015) . They are expected to induce endosomal escape through a variety of mechanisms, including pore formation by endosomolytic agents, membrane fusion by fusogenic agents such as peptides or lipids, and an osmotic pressure-based proton-sponge mechanism Hu et al. 2020) . Non-viral vectors can be utilized with nucleic acids by forming cationic lipid/polymer complexes, cationic or ionizable lipid nanoparticles (LNPs) (Kulkarni et al. 2018) , cell penetrating peptides (CPPs) such as RALA peptides (McCaffrey et al. 2016) , or by bioconjugating with N-acetylgalactosamine (GalNAc) (Hu et al. 2020) . Several non-viral vectors and their characteristics are described in Table 1 . The structure or composition of nanocarriers can be optimized with mRNA followed by characterization such as net charge, size, and pKa of the delivery system (Yin et al. 2013; Yang et al. 2017; Ahmad et al. 2019 ). These non-viral vector systems have shown enhanced efficiency for delivering nucleic acid drugs with less toxicity (Hardee et al. 2017; Kulkarni et al. 2018) . Furthermore, by conjugation with a specific ligand to a nanocarrier capable of interacting with a surface receptor of a target cell, a nucleic acid drug can be more selectively delivered to a target tissue or cell, thus increasing its therapeutic efficacy and reducing side effects (Kim et al. 2014; Hu et al. 2020) .

The escape of nucleic acids from endosome or lysosome into the cytoplasm in cell has been an important issue in recent years . Several studies have found that only a very small fraction (less than 2%) of endocytosed nucleic acids can escape into the cytoplasm (Gilleron et al. 2013; Kim et al. 2019) . Several strategies for endosomal escape have been evaluated. One of such strategies using pH-sensitive components such as ionizable lipids has been shown to be able to enhance the escape of nucleic acids from endosome (Pack et al. 2005; Ahmad et al. 2019) . Its mechanism is called proton-sponge mechanism. When the pH inside the endosome or lysosome changes, the pH-sensitive unit becomes positively charged, allowing waters and ions to flow into the endosome due to increased osmotic pressure, which causes endosomal instability and escape of nucleic acids (Ahmad et al. 2019 ). In addition, various endosomolytic agents are being tested with materials such as peptides, toxins, polymers, and small molecules (Ahmad et al. 2019 ). However, cytotoxic effects of these agents significantly limit their applications. Recently, Herrera et al. (2021) have shown that cholesterol substitution in LNPs can enhance endosomal escape of mRNA encoding a galectin8-GFP reporter protein capable of tracking damaged endosomes, supporting the strategy of modifying the structural relationship of components interacting with sterol components.

LNP is a lipid-based carrier composed of lipids, phospholipids (for structure support), cholesterol (stabilizing agents), and polyethylene glycol (PEG)-lipids (increase half-life) (Cullis and Hope 2017) . Depending on charge properties of LNP at neutral pH conditions, LNPs can be classified into three types: ionizable LNPs, cationic LNPs, and neutral LNPs. Ionizable LNPs appears in an uncharged state throughout circulation. However, in low-pH environments such as endosomes and lysosomes, they can be protonated to contribute to endosomal escaping of nucleic acid to the cytoplasm as mentioned above (Ahmad et al. 2019; Hu et al. 2020) . Cationic LNPs usually show positive surface charge in circulation. They have immunogenicity or interactions with serum proteins induced by static electricity, resulting less efficiency than ionizable LNP (Hu et al. 2020) . Cationic LNPs also show higher in vivo toxicity than ionizable LNPs. As a result, most pharmaceutical companies and institutes are devoting a lot of resources to the development of novel ionizable lipids.

For siRNA, conjugation with a nanocarrier or siRNA with some ligands has been well demonstrated as a good delivery strategy (Osborn and Khvorova 2018; Hu et al. 2020 ). These conjugated siRNAs can enhance their delivery and cellular uptake, promote targeting due to ligand properties of conjugation molecules, and improve dose efficiency with fewer side effects (Hu et al. 2020 ). In addition, as compared to LNP, conjugation has the advantage of having smaller size with less toxicity and immunogenicity (Osborn and Khvorova 2018; Hu et al. 2020) . N-acetylgalactosamine (GalNAc) sugar is a representative conjugation molecule. Two commercially available siRNA drugs (givosiran and lumasiran) employ this platform. GalNAc has ligand properties for Table 1 Non-viral vectors and physical delivery systems PEI, polyethylenimine; PLGA, poly(lactic-co-glycolic acid); PEG, polyethylene glycol; DOTMA, 1,2-di-O-octadecenyl-3-trimethylammonium-propane; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOTAP, 1,2-dioleoyl-3-trimethylammonium-propane; LNP, lipid nanoparticle; DLin-DMA, 1,2-dilinoleyloxy-N,N-dimethyl-3-aminopropane; DLin-MC3-DMA, (6Z,9Z,28Z,31Z)-heptatriaconta-6,9,28,31-tetraen-19-yl-4-(dimethylamino) butanoate; DSPC, 1,2-distearoyl-sn-glycero-3-phosphocholine Gene gun The fine particles containing nucleic acids are shot to enhance their intracellular penetration Gene gun shows good efficiency but causes tissue damage. Deliverable depth is limited, and additional equipment is required specificity on asialoglycoprotein receptor which is widely distributed in the liver. It has demonstrated an enhanced efficacy with a long half-life, making it an important platform for siRNA delivery (Zhang et al. 2021 ).

Physical delivery systems of nucleic acids can be classified according to their delivery method which might be direct injection, electroporation, gene gun, or MNs. Although a physical delivery system uses a straightforward delivery process to enhance delivery efficiency by a series of mechanisms, it can show limitations with single utilization itself since it can cause instability related to naked nucleic acid drugs previously discussed or tissue damage which may cause apoptosis of cells Pardi et al. 2018 ). Physical delivery system coupled with a non-viral vector can be used as a possible strategy to deliver nucleic acids into cells more effectively than when either a physical delivery system or a non-viral vector is used alone (Huang et al. 2018 ).

Physical delivery system using electroporation method can cause electrical pulses to cells or tissue to form pores on their membranes so that nucleic acid drugs can be delivered directly to the cytoplasm, enabling bypass of endosomerelated problems (Pardi et al. 2018; Bernelin-Cottet et al. 2019) . Gene gun is a kind of microparticle delivery system with a needle-free method. It takes gene-loaded microparticles to cells/tissues through an accelerator. However, these methods can significantly damage tissues and cause cell death Pardi et al. 2018 ). In addition, they require expensive equipment.

The MN system is a one of transdermal drug delivery systems that can promote drug permeation through skin by punching a physical channel through a metal or polymer needle of 1500 μm or less on the stratum corneum layer which is considered the largest barrier for transdermal delivery (Kaushik et al. 2001; Arora et al. 2008; Chen et al. 2016) . With minimal invasiveness, permeation of nucleic acid drugs such as pDNA and mRNA as well as other macromolecules can be greatly enhanced with less pain (Kim et al. 2013; Yin et al. 2013) . This straight method can be applied by selfadministration without a medical expert help, thus increasing user convenience and providing enhanced stability of the nucleic acid drug due to a dried formulation (Pearton et al. 2012; McCaffrey et al. 2016) . According to drug loading location and properties as shown in Fig. 1, MN can be divided into four types: solid MN, coated MN, dissolving MN, and hollow MN . Characteristics of MNs used as nucleic acid delivery systems are described below and summarized in Table 2 .

To enhance drug penetration, solid MN is composed of metal, silicone, polymer, or ceramic to pierce the skin before or after casting the drug solution on the skin. It is generally manufactured using a dry or wet etch technique (Chabri et al. 2004; Martanto et al. 2004; Birchall et al. 2005) . Because solid MN does not contain drugs, administration method using solid MN may be accomplished in two methods: 1) Apply a drug-loaded solution/gel on the skin and then apply the solid MN, 2) Apply solid MNs on the skin and then apply a drug-loaded solution/gel loading. When these two solid MN application methods were compared using a reporter gene encoding plasmid DNA (Yan et al. 2014) , the method of applying the microneedle after applying the plasmid solution showed 87 times higher luciferase expression than the method of applying the microneedle before applying the plasmid solution.

Solid MN is expected to have great penetration efficiency since it employs a material like metal or silicon to provide strong mechanical characteristics. However, fine fragments such as a sharp tip may be left as a residue in the skin. Furthermore, the dosage given to the body is significantly lower 

Coated MN is manufactured by dipping or spraying a solution containing a drug onto the surface of a solid MN (Gill et al. 2010; Li et al. 2021) . The drug and polymer vehicle (such as sodium carboxymethylcellulose) on coated MN dissolve quickly after epidermal application, allowing the loaded medication to permeate into neighboring tissues or the bloodstream. Unlike solid MN, coated MN does not require an extra step such as drug solution deposition on skin before or after MN application. In addition, coated MN has less drug loss in the administration procedure than solid MN, and adding a stabilizer such as trehalose can enhance the drug's stability (Pearton et al. 2012; Kim et al. 2013) . Several coating techniques, such as dip coating, inkjet printing, drop coating, or spray coating, for manufacturing coated MN have been demonstrated and characterized in a recent review paper (Ingrole and Gill 2019) . The coating height may be changed based on the dipping depth (Gill and Prausnitz 2007) . The dose of the nucleic acid might depend on the number of dipping (Pearton et al. 2012) . The coating efficiency can be enhanced utilizing a layer-by-layer coating technique and the electrostatic principle (Demuth et al. 2013; Kim et al. 2014; Seok et al. 2017; Duong et al. 2018a; Li et al. 2019b ). According to a previous report, the number of dipping can increase the dosage of loaded pDNA on the coated MN. It has been confirmed that coated MN array has a content of 100 μg (Pearton et al. 2012 ). However, when manufacturing coated MN with dipping, a high-concentration (8 mg/mL) of drug solution may need to be prepared ), inducing considerable loss or waste by excessive consumption of nucleic acid. In addition, sharp tip pieces of MN components may remain in the skin, as shown in solid MN. Since the MN and active pharmaceutical ingredients (APIs) are always in contact, the compatibility between the MN material and APIs should also be considered.

When applied to the skin, dissolving MN is rapidly dissolved by the tissue fluid, releasing drug molecules. Dissolving MN is composed of biodegradable polymers or saccharides, containing drug vehicle inside the MN. Because it contains drug molecules, dissolving MN, like coated MN, can be administered in a single step. This can be manufactured using a micro-molding technique or a drawing lithography. The loading dose of dissolving MN is considered to be greater than that of coated MN ). In addition, accurate and flexible dose adjustment is possible. There is no concern about the sharp tip of metal MN remaining in the skin. Because release properties of drugs from dissolving MN are greatly affected by the composition of polymers and additives, its therapeutic efficacy could be enhanced by controlling release properties of dissolved MN. However, the mechanical strength of the dissolving MN must be sufficient enough to penetrate the skin. In addition, dissolving MN is sensitive to storage conditions owing to moisture, which might affect its mechanical strength. It is also critical to consider degradation of the polymer that is absorbed into the body and biocompatibility of biodegradable polymers as these aspects can severely limit the range of materials. Waste of drugs in the manufacturing process, such as sidewalls of MNs and the baseplate part of MN considered less bioavailable than the needle tip itself appears to be considerable (approximately 50% of drugs are discarded after manufacturing) (Cole et al. 2017 ). Thus, a manufacturing process using a micro-molding system should be developed to minimize such drug loss.

A hollow MN is often a MN into which a liquid drug from the reservoir located underside of MN is delivered via a needle tube or an opened channel (Hickerson et al. 2013 ). The flow rate and pressure are generally controlled by a separate device (Mansoor et al. 2015; Bae et al. 2019; Cárcamo-Martínez et al. 2021) . A comparatively large amount of drug can be delivered precisely with a hollow MN than with other types of MN. However, exceeding the threshold pressure or flow rate may induce backflow through the side wall around the needle (Mansoor et al. 2015) . Therefore, it needs to be delivered with a suitable pressure and speed, this limiting the delivery rate. A flow rate of 200 nL/min used as a reference appears to be inefficient (Mansoor et al. 2015) . Furthermore, because the drug is delivered in a liquid form, the stability of the drug may cause a problem compared to coated or dissolving MNs with a dry condition for encapsulated drugs ( 

Many studies have proven the efficacy of nucleic acid drug delivery systems using various types of MNs (Table 3) . Figure 2 shows schematic configurations of several types of MN systems and physical delivery systems combined with MN for nucleic acid drug delivery. Many of them have focused on nucleic acid vaccine for cancer/infectious diseases. Several investigations have compared the effectiveness of MN systems for nucleic acid drug delivery to other delivery routes by adopting reporter gene encoding or reporter gene silencing nucleic acids (González-González et al. 2011; Demuth et al. 2013; Dul et al. 2017; Liang et al. 2020; Liu et al. 2021 ). A MN system coupled with well-designed nonviral vectors, or a physical delivery method often exceeds the MN alone with naked nucleic acid delivery. This section discusses recent advances in MN-based nucleic acid vaccination for infectious diseases or cancer. Furthermore, we highlighted research on the effects of MN morphology or formulation on the efficacy of nucleic acid-encapsulated MNs. In addition, studies on MN combined with other nucleic acid delivery systems are described. Finally, limitations and considerations for developing MNs for nucleic acid delivery are discussed.

Because of the abundance of immune cells in the skin, conventional antigen vaccines and nucleic acid vaccines can successfully elicit an immune response with an MN system. Prow et al. (2010) have visualized MN-induced migration of thousands of antigen presenting cells (APCs) in epidermal area of mice after activating with a conventional antigen or DNA vaccine through MN application (Prow et al. 2010) . In addition to serving as a delivery system, MN system may serve as a physical adjuvant by increasing cell death-induced immunity during application Takeuchi et al. 2016 ). Furthermore, because of its benefits of stability and simplicity, using MN is considered as a practical and efficient method for nucleic acid vaccination.

Compared to traditional delivery methods such as intramuscular (IM) or subcutaneous (SC) injection, MNbased nucleic acid vaccines can produce efficient immune responses (Gill et al. 2010; Kim et al. 2012; Song et al. 2012; Ma et al. 2014; Fernando et al. 2016; Yang et al. 2017; Liao et al. 2017; Moreno et al. 2017; Duong et al. 2018b Duong et al. , 2020 Zhang et al. 2018; Yan et al. 2018) . Song et al. (2012) have demonstrated that naked influenza hematogglutinin (HA)encoding plasmid DNA coated onto metal MN has protective effect. Furthermore, the dose utilizing MN system may be reduced more than five times when compared to IM. Yang et al. (2017) have reported that the thermostability of pDNA vaccine using a dissolving MN system for six weeks by encapsulating pDNA vaccine against Ebola virus with poly(lactic-co-glycolic acid) based nanoparticles carrier is enhanced. The pDNA MN for Ebola virus demonstrated higher IgG titer than an IM route, showing the efficacy of Ebola immunization using MN-based pDNA vaccine. In another research, Liao et al. (2017) have fabricated a dissolving MN vaccine utilizing a pDNA polyplex coupled with cationic branched PEI (polyethylenimine), which is 3.5 times more efficient than IM.

The effectiveness and potentials of nucleic acid based cancer vaccines using MN system by loading tumor-associated antigen-encoding mRNA, pDNA, or siRNA which interferes with mRNA encoding tumor associated protein into MN have been demonstrated (Hooper et al. 2007; Prow et al. 2010; Gill et al. 2010; Song et al. 2012; Kim et al. 2012 Kim et al. , 2014 Ma et al. 2014; Kines et al. 2015; Fernando et al. 2016; Ali et al. 2017; Pamornpathomkul et al. 2017; Xu et al. 2017; Cole et al. 2018 Cole et al. , 2019 Koh et al. 2018; Pan et al. 2018; Ruan et al. 2018; Duong et al. 2018b Duong et al. , 2020 Li et al. 2019b ). Cole et al. (2019) have utilized a prostate stem cell antigen (PSCA) encoding pDNA with dissolving MN using RALA peptide (one of the cell penetrating peptides) nanoparticle carrier to establish prophylactic and therapeutic efficacy. Li et al. (2019a) have generated pH-triggered release to promote rapid release at a relatively low pH (5.5) by coating PEI and p53-encoding pDNA on a pH-responsive poly-electrolyte multilayer (PEM), confirming its tumor suppression ability.

Several research studies have used adjuvants with MNbased nucleic acid vaccinations (Zhou et al. 2010; Qiu et al. 2016; Xu et al. 2017; Duong et al. 2018b Duong et al. , 2020 . Adjuvants can boost the vaccination effectiveness by inducing additional immunological response. Adjuvants such as poly I:C (Duong et al. 2018b (Duong et al. , 2020 and paclitaxel (Xu et al. 2017) have been used in some cases for MN-based cancer vaccines. It has been demonstrated that the immunogenicity of MNbased vaccine is improved in the group with adjuvant added.

In one work, photothermal therapy with IR820 NIR dye has been used in a hyaluronic acid based dissolving MN encapsulating p53-encoding pDNA. This complexed system substantially suppressed the growth of melanoma (Xu et al. 2020 ).

Since MNs operate by physically punching a hole in the stratum corneum, mechanical strength and morphology known to directly impact penetration properties of MN are critical for efficient delivery. Solid MN and coated MN are largely free of these issues in terms of mechanical strength because these MNs are composed of strong enough materials such as metals. Nevertheless, mechanical strength is an essential factor for dissolving MN which is made of polymer or sugar. Mechanical strength of dissolving MN can be influenced by formulation, storage conditions such as humidity, and structure . Dissolving MNs fabricated with four other biocompatible polymers (hyaluronate, chitosan, gelatin, or polyvinyl alcohol) were evaluated in this study. The findings Pan et al. (2018) suggest that mechanical strength and in vivo mouse skin insertion ability may differ significantly depending on the MN material under high humidity conditions, emphasizing the importance of formulation and optimal storage conditions for reproducible administration of dissolving MNs. In addition, morphological characteristics have substantial impacts on MN penetration regardless of the needle type (Gittard et al. 2013) . It has been shown that morphological characteristics such as needle length are critical as they may impact transfection efficiency in nucleic acid delivery (Yan et al. 2014; Koh et al. 2018; Wang et al. 2020 ). Transfection efficiency and kinetics of naked mRNA depending on needle height have been demonstrated. The longer MNs encapsulating the naked mRNA encoding luciferase (one of the reporter genes) demonstrated better transfection efficiency with a longer duration than the shorter MNs in the (Koh et al. 2018) . Similarly, Yan et al. (2014) have examined in vivo expression of reporter genes encoding pDNA by varying the height of solid MN to 0.25, 0.5, and 0.75 mm. They also found that longer needles and longer application durations could lead to a more efficient transfection. Wang et al. (2020) have evaluated siRNAloaded dissolving MN by varying the length of the drugloaded tip according to hyaluronic acid concentration and suggested that increasing the length of the tip itself to which the drug is exposed might lead to a more efficient nucleic acid exposure to antigen presenting cells. This suggests that more cell contact induced by longer needles can lead to a more effective expression, although this remains further investigation (Yan et al. 2014; Wang et al. 2020) . Although formulation is a crucial part in any pharmaceutical approach, studies on the formulation of MN associated with nucleic acid therapeutics are very rare, with many unclear parts despite decades of research (Cole et al. 2017) . The transfection efficiency of nucleic acid drug in dissolving MN formulations can be greatly affected by the type of polymer and the additive content Cole et al. 2017; Koh et al. 2018) . The most crucial factor to consider is the selection of a polymer that is compatible with nucleic acid. In the case of selecting a biodegradable polymer to fabricate dissolving MN, available options are quite restricted, with the risk of DNase and RNase contamination. Because further contamination is possible during the fabrication process of microneedle, the integrity of the nucleic acid must be validated when blending with polymer and after fabrication of microneedle. It should be noted, however, that the transfection efficiency of a nucleic acid might be significantly diminished by several polymers, even when the nucleic acid integrity in the formulation is adequate. In a nucleic acid MN formulation research, the recovery and integrity of pDNA encapsulated in MNs was evaluated by screening four polymers utilized in the manufacturing process of soluble MNs that can form complex with reporter gene-encoding pDNA through RALA peptide (Cole et al. 2017 ). In addition, the efficiency of in vitro transfection was evaluated. According to molecular weight, two varieties of polyvinylpyrrolidone (PVP) and two types of polyvinyl alcohol (PVA) were examined in that study. A conformational change of the nucleic acid was observed in the pDNA of the PVP-based formulation after 7 days of fabrication. The pDNA encapsulated in the PVP-based formulation had a lower transfection efficiency than the PVA-based formulation in this regard. In another example, naked mRNA was administered with a dissolving needle using polyvinylpyrrolidone (Koh et al. 2018) . Although the mRNA in the PVP-based formulation maintained its integrity for 15 days, the in vivo transfection efficiency decreased as the PVP concentration was increased. In the case of coated MN, carboxymethyl cellulose (CMC) is frequently used to enhance the loading quantity by increasing the viscosity in coated MN. However, according to one research, CMC exhibited a significant decrease in pDNA expression ). Based on the increased particle size of DNA in sodium CMC solution, the authors of this study suggested that DNA aggregation in sodium CMC solution might be the cause of the decrease in transfection efficiency. Because the mechanism of how a polymer influences nucleic acid remains largely unknown, more research is required. Alternatively, in the case of restricted polymer alternatives, screening is necessary to determine the optimum choice that can maximize efficiency.

In the case of coated MN formulation, improvements have been made not only in the coating process, but also in the coating technique and MN structure to enhance the limited dose and transfection efficiency. To increase the dose of a nucleic acid drug, which was previously considered to be a limitation of coated MN, the number of dipping (Pearton et al. 2012) , nanopatterning (Jung et al. 2017 ), multilayered coating system (Demuth et al. 2013; Kim et al. 2014; Seok et al. 2017) , and other parameters have been examined. The multiple dipping method, as described in the coated MN section, significantly increased the nucleic acid loading dosage to about 100 μg. However, as the number of dips increased, the nucleic acid dose uniformity appeared to deteriorate, and the performance of MN may be affected by changes in MN shape caused by coating, such as sharpness (Pearton et al. 2012; Vora et al. 2018) .

A salinized layer with multiple amine groups that show a partially positive charge was introduced to the surface of coated MN to constitute a multilayer with nucleic acid to improve coating efficiency by utilizing the negative charge of nucleic acid in research on multilayered (layer-by-layer) coated MN (Seok et al. 2017) . Kim et al. (2014) also fabricated coated MNs by assembling positively charged albumin and negatively charged heparin on the MN surface at pH 4.0. The authors demonstrated pH-dependent rapid release of plasmid DNA by inducing charge inversion of albumin (isoelectric point of 4.9) to a negative charge at physiological pH. In the Alzheimer's disease vaccination study, the multilayered MN group had a better immune response than the subcutaneously administered group, indicating that the multilayered MN system was an effective delivery system. However, the impact of the release property, in which the drug is dissolved from the MN itself, on transfection efficiency is still insufficient in detailed theory. In one study, the MN system with sustained release properties of nucleic acid produced a better immune response than intramuscular vaccination (Kumar et al. 2012) . Thus, further research on release properties is needed.

Several studies on effects of MN components on the stability of encapsulated nucleic acid on the surface or inside of MN have revealed that several disaccharides (trehalose, sucrose, maltose) (Pearton et al. 2012; Seok et al. 2017; Yang et al. 2017; Cole et al. 2018) , carbohydrate-enriched formulations (Chong et al. 2013) , and some polymers (Cole et al. 2017) can increase stability. Simultaneously, as the use of non-viral carriers, particularly lipid nanoparticles, increases, new strategies for improving stability are required (Schoenmaker et al. 2021) . The approach might involve the use of antioxidants or chelators to control catalysis by reactive oxygen species (ROS) and metal ions, or it could involve formulation of helper lipids required for LNP stability (Pardi et al. 2018) . The issue of mRNA stability has recently grown severe enough to impact the availability of nucleic acid medications beyond conventional storage and shipping issues, highlighting the importance of ensuring stability which demands constant attention (Schoenmaker et al. 2021 ).

With a few exceptions, many MN-based nucleic acid delivery systems coupled with other non-viral vectors or physical delivery systems such as electroporation and gene guns show improved performance compared to MN-based naked nucleic acids.

Nucleic acid complexed with non-viral vectors, notably cationic lipoplex such as DOTAP (Haigh et al. 2014; Bellefroid et al. 2019; Liang et al. 2020) , lipofectamine (Saurer et al. 2010; Kim et al. 2012; Pearton et al. 2012; Chong et al. 2013; Yan et al. 2014; Dul et al. 2017; Pamornpathomkul et al. 2017 Pamornpathomkul et al. , 2018 Yang et al. 2017) , cationic polymer such as polyethylenimine (PEI) (Yin et al. 2013; Hu et al. 2014; Yan et al. 2014; Jung et al. 2017; Liao et al. 2017; Seok et al. 2017; Duong et al. 2018b; Pan et al. 2018; Li et al. 2019b Li et al. , 2021 , and RALA peptide (McCaffrey et al. 2016; Ali et al. 2017; Cole et al. 2017 Cole et al. , 2018 Cole et al. , 2019 have been reported. The LNP system was designed after several characterizations based on the vector to nucleic acid ratio (such as gel retardation assay, particle size, zeta-potential). Welldesigned nanocarriers for nucleic acids have demonstrated better transfection efficiency in most cases than naked ones in vitro and in vivo. In recent years, many nucleic acid delivery systems have employed ionizable LNP including cholesterol and PEG-lipids due to their low toxicity and great efficiency compared to conventional cationic LNPs. However, they have not yet been combined with MN system. Nonviral vector systems are developed constantly. Significant safety concern about an LNP system has just been resolved through worldwide inoculation. More advanced vectors with MN may show promising future, considering the synergistic impact of several current non-viral vector combined systems. Nevertheless, in a study of Yan et al. (2014) , a nucleic acid drug solution was casted and then motorized solid MN was administered with naked, lipoplexed (lipofectamine), or polyplexed (PEI) with reporter-gene expressing pDNA on rat skin tissue in vivo. Results revealed that naked pDNA via solid MN was expressed the most efficiently. However, given the considerable amount of evidence showing that a combined system has better results, this case may suggest the importance of a well-designed nanocarrier system even when variables might have influences. In addition, in the case of lipoplex, it is possible to impair transfection efficiency by forming aggregates in the process of drying or storage (Chong et al. 2013) . Thus, it is also important to examine the effect of drying or carriers that occurs during the manufacturing process of MN on the stability of the system (Crommelin et al. 2021) .

Studies utilizing a combination of MN systems with other physical methods such as gene guns (Gill et al. 2010; Zhang et al. 2013 Zhang et al. , 2014 Zhang et al. , 2015 or electroporation (Hooper et al. 2007; Choi et al. 2010 Choi et al. , 2012 Daugimont et al. 2010; Lee et al. 2011; Wei et al. 2014; Pamornpathomkul et al. 2017; Huang et al. 2018 ) have been reported, leading to a substantial improvement in permeation (Pamornpathomkul et al. 2017) or transfection efficiency compared to a single method (Lee et al. 2011; Choi et al. 2012; Huang et al. 2018) . To increase the delivery efficiency of nucleic acid drugs, the convergence of an MN system with various physical delivery technologies should be continuously studied and developed.

Despite their great potential, there are several considerations and challenges that must be overcome before microneedles for nucleic acid delivery can be commercialized and used in clinical. First, nucleic acid drug stability in the microneedle should be considered. Biopharmaceuticals that are dispersed in liquid formulations frequently promote degradation, and there is also a risk of microbial growth. The dissolving MN or coated MN could minimize these stability issues by removing the solvent, making it a viable strategy for extending nucleic acid shelf life (Pearton et al. 2012; Demuth et al. 2013; Cole et al. 2017; Tucak et al. 2020 ). However, even if the nucleic acid integrity in the formulation is adequate, several polymers can significantly reduce the transfection efficiency of a nucleic acid Cole et al. 2017; Koh et al. 2018 ). More research is needed because the mechanism by which polymers influence nucleic acids is largely unknown. Furthermore, optimal MN formulations that can stabilize nucleic acids at room temperature, as well as appropriate sterilization processes that do not damage the nucleic acids in MN, must be developed. Second, the MN delivery system should be reproducible to deliver defined amounts of nucleic acid drugs consistently. Patient-related parameters such as age, gender, and patient condition influence the amount of drug delivered because the skin penetration rate of the microneedle varies depending on pressing force and training experience. To minimize the impact on the relevant patient-related parameters, optimizing the geometry and arrangement of the MN (Makvandi et al. 2021) , as well as the development of the applicator (Lahiji et al. 2015) , should be considered. Moreover, biomimetic microneedles can improve drug delivery reproducibility by increasing needle adhesion , and drug localization at the needle tip (Jun et al. 2018; Li et al. 2019a) can reduce patient-related variables.

In recent years, nucleic acid drugs have shown great promise beyond the limitation of conventional small molecules or protein therapeutics. In particular, recent vaccination research and current COVID-19 pandemic highlight the importance of nucleic acid therapies as a novel vaccination platform, unlike the conventional vaccine development system with huge limitation in rapidly responding to emerging infectious diseases with constraints of regulation, development, and production. As a result, there is a growing demand for more advanced nucleic acid drugs that can quickly respond to various diseases including infection, cancer, and intractable diseases.

Nucleic acid drugs shown so far are promising. However, they have shown limited stability related to recent cold-chain storage issues (Schoenmaker et al. 2021) . They also need to improve their efficacies. Furthermore, the growing severity of epidemics highlights the need for more effective, selfapplicable, and safe delivery methods. MN-mediated nucleic acid drug delivery is a promising platform for painless and less invasive nucleic acid drug delivery. Compared to the traditional injection approach, it shows enhanced efficacy and improved patient convenience through self-administration with less pain. It also shows increased stability derived from a dried encapsulation, offering a potential alternative to deliver nucleic acid drugs. However, MN systems also face a number of challenges. As with recent mRNA vaccination, relevant regulations must be revised. If clinical relevance is established with efficient manufacturing technology and standardization of repeatable systems, this promise will be realized in the future.

Strategies in the design of endosomolytic agents for facilitating endosomal escape in nanoparticles

DNA vaccination for cervical cancer; a novel technology platform of RALA mediated gene delivery via polymeric microneedles

Micro-scale devices for transdermal drug delivery

Snake fang-inspired stamping patch for transdermal delivery of liquid formulations

In vitro skin penetration enhancement techniques: A combined approach of ethosomes and microneedles

Electroporation of a nanoparticle-associated DNA vaccine induces higher inflammation and immunity compared to its delivery with microneedle patches in pigs

Cutaneous DNA delivery and gene expression in ex vivo human skin explants via wet-etch microfabricated microneedles

Hollow microneedles: a perspective in biomedical applications

Microfabricated silicon microneedles for nonviral cutaneous gene delivery

Microneedles as a delivery system for gene therapy

Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth

Intracellular protein delivery and gene transfection by electroporation using a microneedle electrode array

An electrically active microneedle array for electroporation

Gene silencing following siRNA delivery to skin via coated steel microneedles: In vitro and in vivo proof-of-concept

DNA vaccination for cervical cancer: Strategic optimisation of RALA mediated gene delivery from a biodegradable microneedle system

DNA vaccination via RALA nanoparticles in a microneedle delivery system induces a potent immune response against the endogenous prostate cancer stem cell antigen

Dissolving microneedles for DNA vaccination: improving functionality via polymer characterization and RALA complexation

Minimally invasive cutaneous delivery of macromolecules and plasmid DNA via microneedles

Addressing the cold reality of mRNA vaccine stability

Lipid nanoparticle systems for enabling gene therapies

BNT162b2 mRNA Covid-19 Vaccine in a Nationwide Mass Vaccination Setting

Hollow microneedle arrays for intradermal drug delivery and DNA electroporation

Polymer multilayer tattooing for enhanced DNA vaccination

Transdermal delivery of siRNA through microneedle array

Colocalization of cell death with antigen deposition in skin enhances vaccine immunogenicity

Hydrodynamic gene delivery in human skin using a hollow microneedle device

Microneedle arrays coated with charge reversal pH-sensitive copolymers improve antigen presenting cells-homing DNA vaccine delivery and immune responses

Smart vaccine delivery based on microneedle arrays decorated with ultra-pH-responsive copolymers for cancer immunotherapy

Highly potent intradermal vaccination by an array of dissolving microneedle polypeptide cocktails for cancer immunotherapy

Methods to improve the immunogenicity of plasmid DNA vaccines

Influenza nucleoprotein DNA vaccination by a skin targeted, dry coated, densely packed microprojection array (Nanopatch) induces potent antibody and CD8+ T cell responses

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

Coated microneedles for transdermal delivery

Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine

Image-based analysis of lipid nanoparticle-mediated siRNA delivery, intracellular trafficking and endosomal escape

The effects of geometry on skin penetration and failure of polymer microneedles

Intradermal delivery of synthetic mRNA using hollow microneedles for efficient and rapid production of exogenous proteins in skin

Visualization of plasmid delivery to keratinocytes in mouse and human epidermis

CXCL1 gene silencing in skin using liposome-encapsulated siRNA delivered by microprojection array

4D printing of a bioinspired microneedle array with backward-facing barbs for enhanced tissue adhesion

Advances in non-viral DNA vectors for gene therapy

A new vaccine to battle Covid-19

Species-Specific Recognition of Single-Stranded RNA via Toll-like Receptor 7 and 8. Science (-80

Illuminating endosomal escape of polymorphic lipid nanoparticles that boost mRNA delivery

Gene silencing in skin after deposition of self-delivery siRNA with a motorized microneedle array device

Smallpox DNA vaccine delivered by novel skin electroporation device protects mice against intranasal poxvirus challenge

Therapeutic siRNA: state of the art

A mannosylated cell-penetrating peptidegraft-polyethylenimine as a gene delivery vector

Efficient delivery of nucleic acid molecules into skin by combined use of microneedle roller and flexible interdigitated electroporation array

Elimination pathways of systemically delivered siRNA

Microneedle coating methods: a review with a perspective

Microneedle patch use among those with needle phobia-are all needles the same?

Immediate separation of microneedle tips from base array during skin insertion for instantaneous drug delivery

Nano-patterning of a stainless steel microneedle surface to improve the dip-coating efficiency of a DNA vaccine and its immune response

Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA

Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability

Site-specific recombinase strategy to create induced pluripotent stem cells efficiently with plasmid DNA

Lack of pain associated with microfabricated microneedles

The chemical evolution of oligonucleotide therapies of clinical utility

Advances in intracellular delivery through supramolecular self-assembly of oligonucleotides and peptides

Polyplex-releasing microneedles for enhanced cutaneous delivery of DNA vaccine

Increased immunogenicity of avian influenza DNA vaccine delivered to the skin using a microneedle patch

Cross-protection by coimmunization with influenza hemagglutinin DNA and inactivated virus vaccine using coated microneedles

Vaccination with human papillomavirus pseudovirus-encapsidated plasmids targeted to skin using microneedles

Formulation, characterization and evaluation of mRNA-loaded dissolvable polymeric microneedles (RNApatch)

Lipid nanoparticles enabling gene therapies: from concepts to clinical utility

Microneedle-mediated transcutaneous immunization with plasmid DNA coated on cationic PLGA nanoparticles

Emergence of synthetic mRNA: In vitro synthesis of mRNA and its applications in regenerative medicine

A patchless dissolving microneedle delivery system enabling rapid and efficient transdermal drug delivery

A high-capacity, hybrid electromicroneedle for in-situ cutaneous gene transfer

Rapidly separable microneedle patch for the sustained release of a contraceptive

A gene-coated microneedle patch based on industrialized ultrasonic spraying technology with a polycation vector to improve antitumor efficacy

Cutaneous microenvironment responsive microneedle patch for rapid gene release to treat subdermal tumor

Skin delivery of siRNA using sponge spicules in combination with cationic flexible liposomes

Self-assembly DNA polyplex vaccine inside dissolving microneedles for high-potency intradermal vaccination

Transdermal delivery of adipocyte phospholipase A2 siRNA using microneedles to treat thyroid associated ophthalmopathy-related proptosis

A comparison of plasmid DNA and mRNA as vaccine technologies

Cancer DNA vaccines: current preclinical and clinical developments and future perspectives

Vaccine delivery to the oral cavity using coated microneedles induces systemic and mucosal immunity

Engineering microneedle patches for improved penetration: analysis, skin models and factors affecting needle insertion

A microneedlebased method for the characterization of diffusion in skin tissue using doxorubicin as a model drug

Transdermal delivery of insulin using microneedles in vivo

Microneedles: an innovative platform for gene delivery

Transcending epithelial and intracellular biological barriers; a prototype DNA delivery device

Adeno-associated virus (AAV) dual vector strategies for gene therapy encoding large transgenes

Skin vaccination using microneedles coated with a plasmid DNA cocktail encoding nucleosomal histones of Leishmania spp

Non-viral delivery systems of DNA into stem cells: Promising and multifarious actions for regenerative medicine

The basic science of gene therapy

Improving siRNA delivery in vivo through lipid conjugation

Design and development of polymers for gene delivery

Cationic niosomes for enhanced skin immunization of plasmid DNA-encoding ovalbumin via hollow microneedles

A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin

Intradermal delivery of STAT3 siRNA to treat melanoma via dissolving microneedles

mRNA vaccinesa new era in vaccinology

Brief update on endocytosis of nanomedicines

Microneedle delivery of plasmid DNA to living human skin: formulation coating, skin insertion and gene expression

Safety and efficacy of the BNT162b2 mRNA Covid-19 vaccine

Nanopatch-targeted skin vaccination against West Nile virus and chikungunya virus in mice

DNA-based vaccination against hepatitis B virus using dissolving microneedle arrays adjuvanted by cationic liposomes and CpG ODN

Non viral vectors in gene therapy-an overview

Coated microneedles mediated intradermal delivery of octaarginine/BRAF siRNA nanocomplexes for anti-melanoma treatment

MRNA-based therapeutics-developing a new class of drugs

Layer-by-layer assembly of DNA-and protein-containing films on microneedles for drug delivery to the skin

The Future of Nonviral and Viral Gene Transfer

mRNAlipid nanoparticle COVID-19 vaccines: Structure and stability

Effective humoral immune response from a H1N1 DNA vaccine delivered to the skin by microneedles coated with PLGA-based cationic nanoparticles

The current state and future directions of RNAi-based therapeutics

DNA vaccination in the skin using microneedles improves protection against influenza

The eight human "canonical" ribonucleases: molecular diversity, catalytic properties, and special biological actions of the enzyme proteins

Application of microneedles to skin induces activation of epidermal Langerhans cells and dermal dendritic cells in mice

Microneedles: Characteristics, materials, production methods and commercial development. Micromachines 11:1-30

Novel nanosuspensionbased dissolving microneedle arrays for transdermal delivery of a hydrophobic drug

Upconversion nanoparticle powered microneedle patches for transdermal delivery of siRNA

Effect of humidity on mechanical properties of dissolving microneedles for transdermal drug delivery

A flexible microneedle array as low-voltage electroporation electrodes for in vivo DNA and siRNA delivery

Direct gene transfer into mouse muscle in vivo

Microneedle-assisted, DC-targeted codelivery of pTRP-2 and adjuvant of paclitaxel for transcutaneous immunotherapy

Rapidly dissolving microneedle patch for synergistic gene and photothermal therapy of subcutaneous tumor

Enhancing DNA delivery into the skin with a motorized microneedle device

Enhancement of Ag85B DNA vaccine immunogenicity against tuberculosis by dissolving microneedles in mice

Ebola vaccination using a DNA vaccine coated on PLGA-PLL/γPGA nanoparticles administered using a microneedle patch

Reduced inflammatory response to plasmid DNA vectors by elimination and inhibition of immunostimulatory CpG motifs

Hepatitis B DNA vaccine-polycation nano-complexes enhancing immune response by percutaneous administration with microneedle

An experimental study of microneedle-assisted microparticle delivery

Microneedle Assisted Micro-Particle Delivery from Gene Guns: experiments using skinmimicking agarose gel

Microneedle-Assisted microparticle delivery by gene guns: Experiments and modeling on the effects of particle characteristics

The growth of siRNA-based therapeutics: Updated clinical studies

Microneedles Improve the Immunogenicity of DNA Vaccines

Augmented humoral and cellular immune response of hepatitis B virus DNA vaccine by micro-needle vaccination using Flt3L as an adjuvant