key: cord-1029838-54t6jdcn authors: Williams, Eloise; Isles, Nicole; Chong, Brian; Bond, Katherine; Yoga, Yano; Druce, Julian; Catton, Mike; Ballard, Susan A.; Howden, Benjamin P.; Williamson, Deborah A. title: Detection of SARS-CoV-2 in saliva: implications for specimen transport and storage date: 2020-12-03 journal: J Med Microbiol DOI: 10.1099/jmm.0.001285 sha: d8b23bb5651d6c4cb3e5a09be838e81da530d4e9 doc_id: 1029838 cord_uid: 54t6jdcn Saliva has recently been proposed as a suitable specimen for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Use of saliva as a diagnostic specimen may present opportunities for SARS-CoV-2 reverse transcription polymerase chain reaction (RT-PCR) testing in remote and low-resource settings. Determining the stability of SARS-CoV-2 RNA in saliva over time is an important step in determining optimal storage and transport times. We undertook an in vitro study to assess whether SARS-CoV-2 could be detected in contrived saliva samples. The contrived saliva samples comprised 10 ml pooled saliva spiked with gamma-irradiated SARS-CoV-2 to achieve a concentration of 2.58×10(4) copies ml SARS-CoV-2, which was subsequently divided into 2 ml aliquots comprising: (i) neat saliva; and a 1 : 1 dilution with (ii) normal saline; (iii) viral transport media, and (iv) liquid Amies medium. Contrived samples were made in quadruplicate, with two samples of each stored at either: (i) room temperature or (ii) 4 °C. SARS-CoV-2 was detected in all SARS-CoV-2 spiked samples at time point 0, day 1, 3 and 7 at both storage temperatures using the N gene RT-PCR assay and time point 0, day 1 and day 7 using the Xpert Xpress SARS-CoV-2 (Cepheid, Sunnyvale, USA) RT-PCR assay. The ability to detect SARS-CoV-2 in saliva over a 1 week period is an important finding that presents further opportunities for saliva testing as a diagnostic specimen for the diagnosis of SARS-CoV-2. Saliva has recently been proposed as a suitable diagnostic specimen for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) [1] [2] [3] . Saliva has a number of advantages compared to nasopharyngeal swab (NPS) sampling including (i) reduction of risk to healthcare workers during the close contact and potential aerosol generation involved in collection of NPS, (ii) reduction of patient discomfort involved in NPS collection and (iii) absence of requirements for specialized consumables and trained healthcare workers. Compared to NPS specimens, the sensitivity of saliva for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has ranged between 78-100 % in the published literature [1] [2] [3] [4] [5] [6] [7] [8] . Studies have also demonstrated the feasibility and performance of saliva sampling for SARS-COV-2 as diagnostic specimens in the ambulatory setting [1, 3] . Use of saliva as a diagnostic specimen may present opportunities for SARS-CoV-2 reverse transcription polymerase chain reaction (RT-PCR) testing in remote and low-resource settings, as well as allowing scalable population-level screening. Determining the stability of SARS-CoV-2 RNA in saliva over time is an important step in determining optimal storage and transport times but to date, there are no studies describing the stability of SARS-CoV-2 RNA in saliva. Here, we undertook an in vitro study to assess whether SARS-CoV-2 could be detected in saliva by RT-PCR using three different transport media at different temperatures over a 7 day period. A 'mock' sample matrix was constructed, consisting of 10 ml of pooled saliva that tested negative to SARS-CoV-2 using an in-house E gene RT-PCR using previously published primers [4] . This pooled sample was spiked with gammairradiated SARS-CoV-2 to achieve a final concentration of 2.58×10 4 copies ml of SARS-CoV-2 strain VIC001 [9] , and OPEN ACCESS subsequently divided into 2 ml aliquots comprising: (i) neat saliva; and a 1 : 1 dilution with (ii) normal saline; (iii) viral transport media (University of Melbourne Media Preparation Unit, Melbourne, Australia; product no. 2512), and (iv) liquid Amies medium (University of Melbourne Media Preparation Unit, Melbourne, Australia; product no. 2162). In total, 200 ul aliquots of each contrived sample were made in quadruplicate, with two samples of each stored at either: (i) room temperature (average temperature over the study period of 16 °C) or (ii) 4 °C. At time point 0, day 1, 3 and 7, aliquots underwent RNA extraction using the QIAamp 96 Virus QIAcube HT Kit (QIAGEN, Hilden, Germany) and were eluted in 60 ul. Reverse transcription was performed using the BioLine SensiFAST cDNA kit (Bioline, London, UK) as previously described [9] . cDNA underwent PCR using an in-house real-time assay using previously described primers targeting the SARS-CoV-2 N gene [10] . In addition, 300 ul aliquots of each contrived sample were tested at day 0, 1 and 7 using the Xpert Xpress SARS-CoV-2 assay (Cepheid, Sunnyvale, USA) on the GeneXpert Infinity platform (Cepheid, Sunnyvale, USA), which targets the E and N2 genes [3] . SARS-CoV-2 was detected in all SARS-CoV-2 spiked samples at all time points, and at both storage temperatures using the N gene assay (Table 1) and was not detected in 18 negative control samples comprising six replicates of normal saline, liquid Amies and viral transport media at timepoint 0. In addition, SARS-CoV-2 was detected in neat saliva, and saliva combined with various transport media (Table 1) . One replicate (1/64; 1.5 %) did not have SARS-CoV-2 detected; this was an aliquot of saliva mixed 1 : 1 with liquid Amies and stored at room temperature for 7 days. Moreover, SARS-CoV-2 was detected using the Xpert Xpress SARS-CoV-2 (Cepheid, Sunnyvale, USA) by both the E and N2 gene targets at day 0, 1 and 7 at both storage temperatures, and in all transport conditions (Table S1 , available in the online version of this article). The variability in sensitivity of SARS-CoV-2 detection by RT-PCR in saliva specimens reported in the published literature likely reflects various patient, sampling and analytical factors. The majority of studies show a higher rate of detection of SARS-CoV-2 by RT-PCR in NPS specimens [2] [3] [4] [5] 8] , however some studies have demonstrated a higher rate of detection in saliva specimens compared to paired NPS specimens [7, 11] , or detection of SARS-CoV-2 in a number of saliva samples with paired negative NPS [1, 3, 8] . There is biological plausibility that samples collected from the oral cavity may be an appropriate specimen type, with a recent study demonstrating high ACE2 receptor expression in the epithelial cells of oral mucosa and the base of the tongue [12] . Defining the ideal specimen collection, transport and processing methods for saliva samples is key to optimizing the role of saliva samples in the diagnosis of SARS-CoV-2. The ability to detect SARS-CoV-2 in saliva over a 1 week period is an important finding that presents further opportunities for saliva testing as an initial screening test in hard-toreach populations where there may be few alternative options. Further studies are required to assess the reproducibility of SARS-CoV-2 RNA detection in saliva samples over this period in tropical climates with higher room temperature and humidity and with RT-PCR targets using other regions of the SARS-CoV-2 genome. Coupled with the use of a 'near care' technology such as the Xpert Xpress SARS-CoV-2, this may significantly increase access to SARS-CoV-2 testing in some settings [3] . These include: (i) remote locations with limited healthcare providers, where samples may require significant transit time; Table 1 . SARS-CoV-2 N gene RT-PCR results for saliva samples combined with various transport media and exposed to various storage conditions over a 7 day period (ii) low resource settings in which sampling consumables and personal protective equipment are limited and (iii) vulnerable populations in which nasopharyngeal swab collection is not an acceptable sampling method. Repeat sampling with a nasopharyngeal swab may then be reserved as a second-line test for those with an ongoing high clinical index of suspicion. This study was supported by a philanthropic grant awarded to the Peter Doherty Institute for Infection and Immunity (D.A.W.) from an anonymous donor. Saliva as a non-invasive specimen for detection of SARS-CoV-2 Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study Saliva as an alternate specimen source for detection of SARS-CoV-2 in symptomatic patients using Cepheid Xpert XprCepheid Xpert Xpress SARS-CoV-2 Comparisons of viral shedding time of SARS-CoV-2 of different samples in ICU and non-ICU patients Consistent detection of 2019 novel coronavirus in saliva Saliva is a reliable tool to detect SARS-CoV-2 Saliva is more sensitive for SARS-CoV-2 detection in COVID-19 patients than nasopharyngeal swabs Saliva sample as a non-invasive specimen for the diagnosis of coronavirus disease 2019: a cross-sectional study Isolation and rapid sharing of the 2019 novel coronavirus (SARS-CoV-2) from the first patient diagnosed with COVID-19 in Australia Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR Effect of throat washings on detection of 2019 novel coronavirus High expression of ACE2 receptor of 2019-nCoV on the epithelial cells of oral mucosa The authors declare that there are no conflicts of interest. 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