key: cord-1035031-48yw56zj
authors: Teixeira, Inessa Solek; Leal, Fidel Silveira; Tateno, Ricardo Yudi; Palma, Luiz Felipe; Campos, Luana
title: Photobiomodulation and antimicrobial photodynamic therapies for orofacial lesions in patients with COVID-19: a case series
date: 2021-04-01
journal: Photodiagnosis Photodyn Ther
DOI: 10.1016/j.pdpdt.2021.102281
sha: 516da2673cef083e28f56706242836c8a47db023
doc_id: 1035031
cord_uid: 48yw56zj

Oral lesions related to the novel Coronavirus Disease 2019 (COVID-19) have been increasingly described; however, clinical and epidemiological information is still scant. Although a diversity of therapeutic strategies for the management of these lesions are present in the literature, one can note a lack of standardization and doubtful effectiveness. Thus, the present study aimed to report a series of cases in which a combination of antimicrobial photodynamic therapy (aPDT) and photobiomodulation therapy (PBMT) was used for orofacial lesions in patients suffering from COVID-19. It was noted, in all cases, a marked improvement in tissue repair and pain relief within a few days; moreover, the patients recovered their orofacial functions satisfactorily. Based on the present series of cases and having in mind the conspicuous lack of information on the different aspects of COVID-19, the protocol which combined aPDT with PMBT seemed to be effective in the management of COVID-19-related orofacial lesions.

Severe Acute Respiratory Syndrome Coronavirus 2, a single-chain RNA virus, is responsible for the novel Coronavirus Disease 2019 (COVID-19) pandemic [1] .

According to the World Health Organization website on 19 February 2021, almost 110 million cases of COVID-19 have been confirmed globally since the start of the pandemic, resulting in over 2 million deaths [2] .

A very recent study reported a frequency of oral lesions in patients with COVID-systemic drugs (antifungals, antibiotics, antivirals, corticosteroids) , and even photobiomodulation therapy (PBMT) singly [1] or in combination with antimicrobial photodynamic therapy (aPDT) [5] have been used empirically for treating these orofacial lesions, presenting varying effectiveness.

In light of these facts, the present study aims to report a series of clinical cases in which PBMT and aPDT were used to manage orofacial lesions in patients suffering from COVID-19.

A Brazilian 57-year-old male, with a history of well-controlled hypertension, sought medical help owing to headache, myalgia, and dyspnea. The patient was diagnosed with COVID-19 by the real-time reverse transcription-polymerase chain reaction (RT-PCR) test, and hospitalized with the initial treatment proposal including azithromycin and ceftriaxone; however, after about 12 days, he had to be sent to the Intensive Care Unit (ICU) for endotracheal intubation and mechanical ventilation, lasting 10 days. Besides supportive measures, dexamethasone was administered, as well as piperacillin with tazobactam for Candida albicans present in urine culture and multi-resistant Pseudomonas aeruginosa in tracheal secretion culture.

Approximately 4 days after extubation, it was noted a painful lip lesion covered by crust, which was then removed and caused bleeding (Fig. 1A ). An association of aPDT and PBMT was performed and repeated on the following day. For PBMT, a laser device Laser DUO® (MMOptics, São Carlos, SP, Brazil) was used at 660 nm, on contact mode, point by point, with 100 mW, 33 J/cm2, 0.5 J, and 5 s per point. A total of 6 points were distributed throughout the lesion. Shortly thereafter, an aPDT technique was performed, with methylene blue at 0.01 % applied over all lesions, and after 3 min (time pre-irradiation), the same laser parameters were used, but delivering 40 s (4 J) per lesion. Within 72 hours, the lesion was fully Nineteen days after the first symptoms, even receiving azithromycin and ivermectin, she rapidly developed severe and progressive pneumonia. Thus, after being sent to the ICU for endotracheal intubation and mechanical ventilation, within 30 days was noted painful and extensive lip lesions covered by crust (Fig.   J) . Just after clinical evaluation and diagnosis, the same aPDT and PBMT protocol (described in case 1) were proposed, also daily. However, due to the greater extent of the lesions, more irradiation points were used: 5 points of 4 J on the upper lip and 5 on the lower lip. The complete tissue repair was achieved in 4 days (Fig. 1K) .

In the cases presented, the association of aPDT and PBMT resulted in an important clinical improvement of the patients' orofacial condition within few days.

To the best of the authors' knowledge, only two papers have previously addressed the use of these techniques to treat orofacial lesions in patients suffering from COVID-19. Brandão et al. [6] presented some clinical cases in which the PBMT sessions lasted 10 consecutive days but not all lesions responded satisfactorily to the light approach; however, Ramires et al. [5] combined aPDT and PBMT in extensive lip lesions during a total of 2 days and the patient showed complete healing at the fourth day.

The light protocol applied herein relies on that aPDT is considered to be highly effective in the treatment of opportunistic infections of the oral cavity (including virucidal activity against viruses with an envelope such as the coronaviruses) and that PBMT can reduce or inhibit substances that play important roles in pain and inflammatory processes [5] . Even given the complexity of the referred cases, aPDT and PBMT were shown to be safe and effective in both controlling and treating orofacial lesions in patients who were presenting the signs and symptoms of COVID-19 in different degrees of severity. Furthermore, the technique was simple to perform in a hospital setting and was neither timeconsuming nor expensive.

Concerning the study limitations, case series with no comparison group are ranked at the lowest level of evidence. This kind of study, however, is still highly necessary, especially on COVID-19, since it may provide clinicians early information and novel insights for evolving illnesses. During the rapidly evolving J o u r n a l P r e -p r o o f global pandemic experienced today, any study should not be dismissed, as the entire process of developing, carrying out, and publishing the findings of a highquality study may delay the spread of quite needed data [7] .

Based on the present series of cases and having in mind the conspicuous lack of information on the different aspects of COVID-19, the protocol which combined aPDT with PMBT seemed to be effective in the management of COVID-19related orofacial lesions. 

Oral manifestations of COVID -19 disease: A review article

World Health Organization, WHO Coronavirus Disease (COVID-19)

Oral lesions in Covid 19 positive patients

Oral Manifestations in Patients with COVID-19: A Living Systematic Review

A combination of phototherapy modalities for extensive lip lesions in a patient with SARS-CoV-2 infection

Oral lesions in patients with SARS-CoV-2 infection: could the oral cavity be a target organ?, Oral Surg Oral Med Oral Pathol Oral Radiol