key: cord-1036041-zpgges77 authors: Loinaz, Carmelo; Marcacuzco, Alberto; Fernández‐Ruiz, Mario; Caso, Oscar; Cambra, Félix; San Juan, Rafael; Justo, Iago; Calvo, Jorge; García‐Sesma, Alvaro; Manrique, Alejandro; Pérez‐Jacoiste Asín, María Asunción; Folgueira, María Dolores; Aguado, José María; Lumbreras, Carlos title: Varied clinical presentation and outcome of SARS‐CoV‐2 infection in liver transplant recipients: initial experience at a single center in Madrid, Spain date: 2020-06-20 journal: Transpl Infect Dis DOI: 10.1111/tid.13372 sha: fa8ddde9e33895fa5d2cf5bd37455de58f7d3633 doc_id: 1036041 cord_uid: zpgges77 BACKGROUND: Which are the consequences of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV2) infection in liver transplant (LT) recipients? METHODS: We attempted to address this question by reviewing our single‐center experience during the first two months of the pandemics at a high incidence area. RESULTS: Nineteen adult patients (5 females) were diagnosed by May 5th, 2020. Median age was 58 (range 55‐72), and median follow‐up since transplantation was 83 (range 20‐183) months. Cough (84.2%), fever (57.9%) and dyspnea (47.4%) were the most common symptoms. Thirteen patients (68.4%) had pneumonia in X‐ray/CT scan. Hydroxychloroquine was administered in 11 patients, associated with lopinavir/ritonavir and interferon β in 2 cases each. Immunomodulatory therapy with tocilizumab was used in 2 patients. Immunosuppression (IS) was halted in one patient, and modified in only other two due to potential drug interactions. Five (26.3%) patients were managed as outpatient. Two patients (10.5%) died, 10 (52.6%) were discharged home, and 2 (10.5%) were still hospitalized after a median follow‐up of 41 days from the onset of symptoms. Baseline IS regimen remained unchanged in all surviving recipients, with good liver function. CONCLUSIONS: Our preliminary experience shows a broad spectrum of disease severity in LT patients with COVID‐19, with a favorable outcome in most of them without needing to modify baseline IS. The first case of SARS-CoV2 (Severe Acute Respiratory Syndrome coronavirus 2) infection, now termed as Coronavirus Disease 2019 , was diagnosed in Madrid on February 25th, 2020. Within a few weeks, the infection spread extraordinarily. By the first week of March some surgical procedures were already cancelled in our institution in anticipation of the lack of beds to admit patients with the infection. Actually, our last liver transplant (LT) procedure was performed on March 7th and the abdominal transplants program was closed shortly after. By the end of March, there were around 900 patients admitted with COVID-19 in a hospital that usually has 1200 beds running. Some other transplant programs suffered similar circumstances, shortly before, as in Milano 1 , or afterwards, as in New York 2 , the most punished city in U.S.A. till the end of March 2020. Meanwhile, we started to diagnose SARS-CoV-2 infection in liver transplant recipients in our hospital. The first recipient was admitted to the hospital on March 15th and due to the scarce information available on the effects of this emerging infection in immunocompromised hosts, we started a prospective data collection. Information regarding the clinical presentation and outcome in liver transplant recipients is till the date, limited to small case series and preliminary data from registries. There is a report of a LT recipient with perioperative infection 3 , and two cases during follow-up in China 4,5 , 3 cases from Lombardy 6 , 6 cases from Madrid 7 , 9 from an International Registry 8 , 24 from a survey in Italy 9 and 3 more in the U.S.A. 10-12 . Therefore, we aimed at presenting our preliminary experience involving COVID-19 in the LT population during the first two months of this pandemic crisis in Madrid. This article is protected by copyright. All rights reserved We included adult LT recipients consecutively diagnosed with COVID-19 at our tertiary-care center between March 15th and May 5th, 2020. Diagnosis was made by means of epidemiological, clinical, laboratory and radiological data, and confirmed by RNA amplification. In the case of a PCR negative but showing highly suggestive clinical symptoms, laboratory and radiologic data of COVID-19, the final decision about diagnosis was made if all 3 expert infectious disease specialist (MFR, APJ and RS) agree with the diagnosis. This observational study has been performed with the support of an "ad hoc" database set up since the first LT recipient was admitted, being completed with the information from the hospital electronic medical records (HCIS, DXC technology, Las Rozas, Spain). Demographic and clinical features, laboratory and radiology results, therapy, and recipient and graft outcomes were collected. The local Clinical Research Ethics Committee approved the study protocol (ref. no. 20/151) and granted a waiver of informed consent due to its retrospective observational design. All immunocompromised patients admitted to the hospital were managed following a defined pathway designed by the the Unit of Infectious Diseases, General Internal Medicine, and the different specialties involved in transplant patient care. In the case of LT patients, general surgeons evaluated these patients at the emergency room upon a suspicion of COVID-19 diagnosis and located in a specific area with contact precautions, where they were tested for SARS-CoV-2 through real-time reverse transcription polymerase chain reaction (rRT-PCR). Depending on the clinical situation they were admitted to the hospital, discharged home with presciptions and isolation measures, or to a medicalized hotel if they cannot fulfill isolation measures at home. As there is not a specific and efficacious treatment of COVID-19, the treatment protocol has changed during the study time. The first pneumonia cases were treated with hydroxichloroquine (HC) (400 mg/12 hours during the first day, then 200 mg/12 h during 4-9 days) and lopinavir/ritonavir (LPV/r )(200/100 mg twice a day for 14 days), after oral or written informed consent. Some patients also received subcutaneous β interferon (IFN-β )(250 μg every 48 hours). A single IV dose of tocilizumab (400 or 600mg if body weight < 75 kg or ≥75 kg, respectively) was added in selected cases with excessive inflammatory response (C reactive protein [CRP] levels >15 mg/dL and/or interleukin [IL]-6 levels >40 pg/mL) and worsening respiratory failures (bilateral lung infiltrates, oxygen saturation <92% at room air and/or partial pressure of arterial oxygen [PaO 2 ]/FiO 2 ratio <300). Patients with mild infection could be discharged home with an outpatient HC prescription. Immunosuppressive regimen was adjusted according to the severity of illness and the risk of drug-drug interactions (i.e. LPV/r). Statistical analysis comprised mean ± standard deviation (SD) or the median with interquartile range (IQR) for quantitative data, and absolute and relative frequencies for qualitative variables. Nineteen adult LT recipients with COVID19 were analyzed (one of them had undergone liver-kidney transplantation). Only one had a suspected nosocomial SARS-CoV-2 infection, after being admitted for a liver biopsy and a percutaneous transhepatic cholangiography without any symptoms of COVID-19. The first six patients of this series have been briefly reported in a previous study that also included kidney and heart transplant recipients diagnosed at our institution by March 23th 7 The test was done twice, one at admission and the other 5 days later. His symptoms started one week before being evaluated and most probably he became then rRT-PCR negative or the result was related to swab sampling or less probably to test sensitivity 13 . His spouse had also typical symptoms, one week before the patient. Patient #18 was admitted in March due to cough and diarrhea, with related renal failure. He had a negative PCR result for SARS-CoV-2. Hepatitis A, CMV and C. difficile infection were ruled out. He improved and was discharged after 4 days, but he had shared room with another patient that later had a positive PCR. He has been admitted after 18 days with cough, fever and bilateral pneumonia, highly suspicious of COVID-19, again with negative PCR. Lowest lymphocyte count during admission ranged from 0.2 x 10 9 /L to 1.2 x 10 9 /L. CRP values ranged from 1.47 to 216.1 mg/dl (table 2 shows laboratory results, treatment and outcome). Five patients received outpatient care. HC was prescribed in two of them. One (case #7), asymptomatic when a follow-up CT (ordered as a scheduled follow-up of an incidental hepatocellular carcinoma found in the resected This article is protected by copyright. All rights reserved specimen) showed bilateral ground glass infiltrates ( Figure 2 ). He had mild symptoms afterwards: some weakness, dysthermic feeling without fever and some head pressure without headache. Patient #5 had fever, cough, thoracic pain and dyspnea when seen at the Emergency Department (ED), with a normal chest X-ray. The third patient (#6) lost her mother due to COVID-19, but she only showed transient nausea and vomiting, with weakness and myalgias. Other two patients (#15, #16) were managed by their primary care physicians. All five of them remained at home in isolation. HC was used in 9 of the admitted patients. In 2 with LPV/r , and in one of these with IFN-β. Another patient received HC plus IFN-β, without LPV/r. Two more patients were treated with tocilizumab, one in addition to HC. Both had bilateral pneumonia and high PCR, LDH, ferritin and D-dimer levels (data not shown). None of them showed infectious complications, being discharged after two weeks of admission. Only two patients received high doses of steroids (250 methyl-prednisolone for 3 days, and 60 mg/12 hours, then tapered), one of them treated with tocilizumab. Both patients were discharged in less than two weeks. Patient #4 arrived to a nearby district hospital in a very severe condition, after more than two weeks of symptoms and two previous visits to the ED where he was diagnosed of possible viral pneumonia. He began to show symptoms while he was vacationing at the seaside in other region, before the health personnel were fully aware of the pandemics in our country. He was admitted to the ICU, and rapidly deteriorated, without receiving any specific antiviral treatment. As only five patients were receiving their graft in the previous 2 years, most of our patients were receiving a mild immunosuppressive regimen (drug levels nor shown). Fifteen patients were on IS monotherapy, four of them with mTOR inhibitors and seven with MMF/MPA. IS was halted in patient #4 when admitted in the ICU. Two patients (# 1,2) treated with LPV/r were under everolimus before admission. They were shifted to prolonged-release tacrolimus, in very low dose (0,5 mg/week) due to the CYP3A4 inhibition by ritonavir 14 Two patients (10.5%) died before the end of 3 weeks of symptom onset. Both due to respiratory failure, though the addition of pulmonary embolism was suspected in patient #4. As of May 7, fifteen patients are at home in good condition. Discharged patients stayed from 5 to 27 days, with a mean of 11.4 days. The liver-kidney transplant recipient had the longest admission. He has an ileostomy due to post-transplant complications, he began with olfactory and taste disorders and diarrhea, without respiratory symptoms and had a normal chest X-ray. He stayed without oxygen support, low CRP levels (1.6 mg/dl), with slow improvement of diarrhea and hyponatremia. Only two patients (#13,#18) remained at the hospital at the time of this writing. Patient #13 is the one with hospital-acquired infection, and is recovering from a severe cholangitis and without showing any symptoms of COVID-19 and with a SARS-CoV-2 PCR negative. He is waiting to be transferred to a nearby chronic care Accepted Article hospital. Patient # 18 had been recently admitted, and he is now needing nasal cannula oxygen support, correctly evolving, with good prognosis. None of the patients was readmitted excepting the one previously admitted for diarrhea. There is no case of recurrence. All living patients are with the same immunosuppression treatment that had before the infection, with good liver function. This article is protected by copyright. All rights reserved Little is known yet about COVID19 infection in LT recipients. Transplant patients are considered at risk for any kind of infection, and this risk is a function of two factors: epidemiological exposures and the net state of IS 15 . The exposure to SARS-CoV-2 has been very high in our area during the recent weeks, with more than 61500 cases confirmed by PCR in the region of Madrid 16 , out of a population of 6.6 million (0.95%) 17 . Thus, the rate of infection in our liver recipients is quite similar to the general population, 19 out of 1200 living patients. In our series, factors contributing to the net state of IS are immunosuppresive therapy, that was relatively low in the majority of our patients due to the long median follow-up since transplantation,. On the other hand, comorbidities were present in many of them, mainly diabetes, and advanced age which have been associated with an increasing risk of severe disease in SARS-CoV-2 infection 18 . A recent report from China 19 on two patients, one with a kidney transplant (51years old) and the other with a bone marrow transplant (58y) is quite discouraging. They were severe cases, with respiratory failure and needing mechanical ventilation, and both died. Maintenance IS (mycophenolate mofetil and steroids , and cyclosporine A), was discontinued and methylprednisolone and prophylactic antibiotics were initiated, but still both developed nosocomial bacterial infection. Table 3 . Bin et al described a LT patient that developed a severe COVID-19 pneumonia and survived 4 . IS (tacrolimus) was temporarily withdrawn and treated with low-dose steroids. He also received umifenovir, LPV/r, intravenous immunoglobulin, IFN-α, prophylactic cefoperazone, and nutrition support. The authors suggested that reduction or temporary withdrawal of IS may be beneficial for rehabilitation of immunity. Another recent case from China 5 , showed a rapid progression of respiratory insufficiency, several nosocomial infections and multi-organ failure, dying one month and a half after admission. A 37 years old LT recipient had a perioperative SARS-CoV-2 infection 3 , starting with persistent fever on transplant postoperative day 9. He was treated with oseltamivir, recombinant human granulocyte colony-stimulating factor (rh-GCSF) and intravenous immunoglobulin. Tacrolimus and steroids were maintained though titrated to lower doses, and he received oxygen through high-flow nasal cannula, maintaining a saturation between 95% and 99%. He was discharged 51 days after transplantation. There were 3 deaths in long-term (>10 years) LT recipients at the Istituto Nazionale Tumori in Milano 6 . They were receiving low dose IS, had community-acquired pneumonia, needed oxygen at admission and rapidly developed severe respiratory distress syndrome requiring mechanical ventilation. All died between 3 and 12 days after the onset of pneumonia and tested positive for SARS-CoV-2 by PCR. They were male, older than 65 years, overweight, diabetic, and had hypertension. However, three other patients with a follow-up of less than 2 years since LT that tested SARS-CoV-2 positive had an uneventful disease. In contrast, data on 39 cases collected in two registries 8 did not show significant differences in comorbidity among those patients that died, nine, and those who survived. A recent survey from Italy collected 24 cases in the Northern-Central regions 9 . Seven did not require hospitalization, and mortality affected 21% of the patients. There are 3 recent cases reported from the U.S.A. 10-12 , with good outcome. Lagana et al 12 describe the pathology of a postoperative liver biopsy from an infant whose donor tested positive for COVID-19, what could be the first case of SARS-CoV-2 transmission by a transplant. The biopsy showed moderate acute hepatitis with prominent clusters of apoptotic hepatocytes and associated celular debris. Lobular lyphohistiocytic inflammation, and typical portal features of mild to moderate acute cellular rejection were noted. All but one of our cases have been community acquired. The patient with a posible hospital-acquired SARS-CoV-2 did not show virus related symptoms so far. Excluding the two patients that did nor survive, only other four showed liver enzymes deterioration. There was not any case of biopsy proven acute rejection as they spontaneously improved (one after IF-β withdrawal). The balance between IS and infection progression is very difficult to establish in these patients especially when we do not know much about the pathophysiology of the disease. The efficacy of the currrent antivirals used in the COVID-19 therapy is not clear, but seems low in any case. A recent publication of a trial with LPV/r failed to demonstrate a clear benefit in patients with severe COVID-19 20 . Such results, the ocurrence of drug shortages at our center and the risk of interactions with IS agent lead us to reconsider the role of LPV/r for treating LT recipients. Considering that the first report of pathological characteristics of the patient who died from severe infection with SARS-CoV-2 showed a very high concentration of proinflammatory cytokines 21 , the use of the anti-IL-6-receptor tocilizumab in moderate to severe cases with markers (CRP, and/or IL6 levels) of intense inflammatory response, has been promoted in our institution and elsewhere 11, 22, 23 . For this reason, in our institution, those patients with elevated acute phase reactants and moderate/severe disease are treated with tocilizumab. It is interesting to note that two of our patients had thrombotic events that could be related to the infection. Recent literature supports the fact that SARS-CoV-2 infection could predispose to thrombotic disease 24-25 . This is the reason why prophylactic antithrombotic therapy is now widely used in these patients. In our experience with COVID-19 in LT recipients we have seen a broad spectrum of severity. Two patients died of respiratory failure at the beginning of our experience. Both were male in the 8th decade of life, diabetic and had hypertension. Out of the remaining 17 patients, 5 could be managed as outpatient with close communication with the hospital staff, and the highest oxygen concentration administered was 40% in 2 of the admitted patients that survived . Regarding immunosuppressive drug changes, we stopped mTOR inhibitors in two of our first patients on LPV/r and shifted to tacrolimus in low dose, and MMF was halted in patient 4 shortly before his fatal outcome. All the other patients maintained their IS treatment, and only 2 of them had a transient deterioration of their liver profile that is difficult to know if it is due to drug toxicity or the disease itself, as the IS drug levels were closely monitored (data not shown). The majority of our patients were transplanted long time before the infection, allowing a mild IS. But, based on our experience with the first cases, we maintained the IS even in those 5 patients with a transplant time of less than 2 years, with a good outcome. Our preliminary experience does not permit to know which is the best treatment for LT patients with COVID-19 disease. Our experience with LT recipients and SARS-CoV-2 infection shows a varied picture in terms of clinical presentation and severity. Though 7 of our patients were older than seventy, there were only 2 deaths. IS dose adjustment does not seem needed in long term survivors that usually have low maintenance drug levels. This article is protected by copyright. All rights reserved Coronavirus Disease 2019 and Transplantation: a view from the inside Lest we forget Perioperative presentation of COVID-19 Disease in a liver transplant recipient Successful treatment of severe COVID-19 pneumonia in a liver transplant recipient Fatal outcome in a liver transplant recipient with COVID-19 COVID-19 in long-term liver transplant patients: preliminary experience from an Italian transplant centre in Lombardy COVID-19 in solid organ transplant recipients: a single-center case series from Spain Determining risk factors for mortality in liver transplant patients with COVID-19 Preliminary Analysis of the Impact of COVID-19 Outbreak on Italian Liver Transplant Programs Earliest cases of coronavirus disease 2019 (COVID-19) identified in solid organ transplant recipients in the United States Clinical course of COVID-19 in Liver Transplant Recipient on Hemodialysis and Response to Tocilizumab Therapy: A Case Report COVID-19 Associated Hepatitis Complicating Recent Living Donor Liver Transplantation Correlation of Chest CT and RT-PCR testing in Coronavirus Disease 2019 (COVID-19) in China: A Report of 1014 Cases CYP3A4-mediated lopinavir bioactivation and its inhibition by ritonavir. Drug Metab Dispos Accepted Article This article is protected by copyright Informe sobre la situación de COVID-19 en España nº 28, 4 de mayo de 2020 Pathological findings of COVID-19 associated with acute respiratory distress syndrome Clinical and coagulation characteristics of 7 patients with critical COVID-2019 penumonia and acro-ischemia. Zhongua Xue Ye Xue Za Zhi COVID-19 and Thrombotic or Thromboembolic Disease: Implications for Prevention, Antithrombotic Therapy Author contributions All authors contributed to the concept, data collection, analysis, drafting, and final approval of the manuscript This article is protected by copyright. All rights reserved