key: cord-1038763-m1m431yn authors: Tiede, Andreas; Sachs, Ulrich J.; Czwalinna, Andreas; Werwitzke, Sonja; Bikker, Rolf; Krauss, Joachim K.; Donnerstag, Frank; Weißenborn, Karin; Höglinger, Günter; Maasoumy, Benjamin; Wedemeyer, Heiner; Ganser, Arnold title: Prothrombotic immune thrombocytopenia after COVID-19 vaccine date: 2021-04-30 journal: Blood DOI: 10.1182/blood.2021011958 sha: 327ece3fe2b07f548c01fb4cece078c9dfff0896 doc_id: 1038763 cord_uid: m1m431yn We report five cases of prothrombotic immune thrombocytopenia after exposure to the ChAdOx1 vaccine (AZD1222, Vaxzevria) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Patients presented 5 to 11 days after first vaccination. The spectrum of clinical manifestations included cerebral venous sinus thrombosis (CVST), splanchnic vein thrombosis (SVT), arterial cerebral thromboembolism, and thrombotic microangiopathy (TMA). All patients had thrombocytopenia and markedly elevated D-Dimer. Autoantibodies against platelet factor 4 (PF4) were detected in all patients although they had never been exposed to heparin. Immunoglobulin from patient sera bound to healthy donor platelets in an AZD1222-dependant manner, suppressed by heparin. Aggregation of healthy donor platelets by patient sera was demonstrated in the presence of buffer or AZD1222 and was also suppressed by heparin. Anticoagulation alone or in combination with eculizumab or intravenous immunoglobulin (IVIG)resolved the pathology in three patients. Two patientshad thromboembolic eventsdespite anticoagulation at a time when plateletswere increasing after IVIG. In summary, an unexpected autoimmune prothrombotic disorder is described after vaccination with AZD1222. It is characterized by thrombocytopenia and anti-PF4 antibodies binding to platelets in AZD1222-dependent manner. Initial clinical experience suggests a risk of unusual and severe thromboembolic events. We report five cases of prothrombotic immune thrombocytopenia after exposure to the ChAdOx1 vaccine (AZD1222, Vaxzevria) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Patients presented 5 to 11 days after first vaccination. The spectrum of clinical manifestations included cerebral venous sinus thrombosis (CVST), splanchnic vein thrombosis (SVT), arterial cerebral thromboembolism, and thrombotic microangiopathy (TMA). All patients had thrombocytopenia and markedly elevated D-Dimer. Autoantibodies against platelet factor 4 (PF4) were detected in all patients although they had never been exposed to heparin. Immunoglobulin from patient sera bound to healthy donor platelets in an AZD1222-dependant manner, suppressed by heparin. Aggregation of healthy donor platelets by patient sera was demonstrated in the presence of buffer or AZD1222 and was also suppressed by heparin. Anticoagulation alone or in combination with eculizumab or intravenous immunoglobulin (IVIG) resolved the pathology in three patients. Two patients had thromboembolic events despite anticoagulation at a time when platelets were increasing after IVIG. In summary, an unexpected autoimmune prothrombotic disorder is described after vaccination with AZD1222. It is characterized by thrombocytopenia and anti-PF4 antibodies binding to platelets in AZD1222-dependent manner. Initial clinical experience suggests a risk of unusual and severe thromboembolic events.  Patients presented with a broad spectrum of unusual thromboembolic manifestations after AZD1222 exposure  A triad of thromboembolic events, thrombocytopenia and anti-PF4 autoantibodies was characteristic of the disease The Oxford-AstraZeneca vaccine AZD1222 is projected to play a vital role in combating the COVID-19 pandemic. 1 The vaccine is less expensive and easier to store compared to other vaccines against SARS-CoV-2. 2 Published interim results of four randomized controlled trials in the UK, Brazil and South Africa indicated a favorable safety profile, 3 and the vaccine is now licensed in over 100 countries. Since 15 th March 2021, reports of an unexpected accumulation of thrombotic events in association with thrombocytopenia have emerged and resulted in a temporary suspension of inoculation in several European countries. 4 We report our initial experience in five cases, providing insight into disease pathology, its broad spectrum of clinical manifestations, and response to therapy. This consecutive single-center cohort includes all patients presenting to Hannover Medical School between 8 th March and 4 th April with known or suspected thromboembolic events and thrombocytopenia within 2 weeks after vaccination with AZD1222. Demographic and clinical characteristics are presented in Table 1 . Detailed case descriptions are provided in the supplemental information. Patients or representatives gave informed consent before publication. Anti-PF4 autoantibodies were assessed with enzyme-linked immunosorbent assay (ELISA) and chemiluminescent immunoassay (CLIA). Detailed methods including flow cytometry and modified heparin-induced platelet aggregation (HIPA) assays are described in the Supplement. The patients were women between 41 and 67 years of age and presented 5 to 11 days after their first vaccination with AZD1222 (2.5x10 10 particles). Patient 1 had CVST and progressive signs of TMA including Coombs-negative hemolytic anemia, abundant schistocytes and renal failure. Patient 2 had multiple cortical emboli and aortic arch thrombi. Patient 3 presented with headache and visual disturbance but had no abnormal imaging results. Patient 4 initially presented to a remote hospital without signs of thrombosis but developed a thromboembolic event during the first week. Patient 5 presented with cerebral artery thrombosis. Platelet counts were reduced, and D-Dimer markedly elevated in all patients (comprehensive laboratory data presented in Supplement Table S1 ). None of the patients had previously been exposed to heparin or previous COVID-19. On admission, all patients were SARS-CoV-2 negative by PCR test. Because the clinical picture reminded of heparin-induced thrombocytopenia (HIT), patients were tested for anti-PF4 antibodies but CLIA results were negative. Samples were retested by ELISA and proved strongly positive in all patients ( Figure 1A ). Reactivity was inhibited by heparin in all samples. Sera induced aggregation of normal donor platelets independent of heparin (supplemental Figure S5 ). AZD1222 induced binding of patient immunoglobulin to healthy donor platelets ( Figure 1B ). All patients received anticoagulation with full-dose unfractionated heparin (patient 1) or argatroban (patients [2] [3] [4] [5] . No further treatment was given to patient 3, who recovered quickly. Patients 1, 2 and 5 received a dexamethasone pulse for 4 days. Intravenous immunoglobulin (IVIG, 1 g/kg on two consecutive days) was given in patients 2, 4 and 5 according to ad hoc recommendations by the German, Austrian and Swiss Society on Thrombosis and Haemostasis (GTH). 5 Patient 2 had a favorable outcome with no further events. Patient 4 developed extensive splanchnic vein thrombosis (SVT), when platelets had recovered to 115 /nl on day 6. Patient 5 had a popliteal artery occlusion, when platelets had recovered to 172 /nl on day 8. Eculizumab was given in patient 1 (900 mg weekly) because of TMA and renal failure. Signs of TMA resolved, and platelets recovered over the next week. Eculizumab was also given as a salvage therapy in patient 4 because of a severe thromboembolic event after IVIG and while on anticoagulation. She quickly improved and had no further event. Additional information on treatment and outcome is provided in the Supplement and in Figure 1D . Reviewing our experience with this new kind of prothrombotic complication after vaccination, it is strikingly similar to spontaneous autoimmune HIT. This is an ultrarare disorder occurring in patients never exposed to heparin, and polyanionic substances of bacterial origin have been implicated in its pathogenesis. 6 Although it remains to be demonstrated, how AZD1222 could provoke anti-PF4 autoantibody formation, our observation of vaccine-dependent binding of patient antibodies to healthy donor platelets suggests its direct involvement. Some of our observations may be important for clinical practice. The triad of thromboembolic events, thrombocytopenia and anti-PF4 antibodies appears to be characteristic of the syndrome. This triad was also reported in other cases registered by German medical regulatory agencies. 7 The GTH expert committee currently suggests to screen for anti-PF4 antibodies, if thromboembolic events or thrombocytopenia occurred within 2 weeks after vaccination. 5 Our experience indicates that such antibodies can be detected by HIT IgG ELISA of at least two manufacturers but not by the HIT IgG CLIA that is often used for screening by clinical laboratories. The choice of anticoagulant requires careful consideration. Heparin is strictly avoided in patients with classical HIT, 8 but was apparently harmless in our first patient. In fact, we did not see evidence of heparin enhancing platelet aggregation in our patients. However, caution is required as the polyanion heparin could possibly increase the aggregation potential of anti-PF4 antibodies in some patients, even if antibodies were not caused by heparin itself. Argatroban appears to be a safe and effective alternative. The effect of IVIG is uncertain according to our initial experience. It was suggested by the GTH expert panel because of its reported efficacy in autoimmune HIT. 5, 9, 10 Monomeric IgG in high concentrations can block Fc gamma receptor 2A (FcR2A), thereby inhibiting platelet activation and aggregation by PF4/anti-PF4 immune complexes. 11 Platelet counts improved but two out of three treated patients in our series developed a new major thromboembolic event. Eculizumab is perhaps an alternative but its effect is also uncertain. FcR2A-dependent platelet activation (addressed by IVIG) may not be the only prothrombotic mechanism in this syndrome. PF4/anti-PF4 immune complexes activate the complement cascade. 12 We saw the full picture of TMA in our first patient, reminiscent of atypical hemolytic uremic syndrome (AHUS). Eculizumab (but not anticoagulation alone or plasma exchange) appeared to have reversed the pathology in this patient, suggesting that it was due to uncontrolled complement activation, just like in AHUS. 13 Evidence of complement consumption was noted in patient 4, who received eculizumab because IVIG and anticoagulation had failed. A striking improvement of SVT was noted after eculizumab in this patient. Our series indicates a broad spectrum of disease severity, similar to classical HIT. The presenting symptoms ranged from very mild to unusually severe. Awareness and early treatment can possibly prevent complications as suggested by the example of patient 3, who presented with mild symptoms already on day 5 and improved without sequelae upon anticoagulation. As the knowledge about this syndrome continues to unfold, we suggest a high level of suspicion and prompt testing in patients with thrombocytopenia or signs of thromboembolism after vaccination. Despite the suggestive temporal relationship between vaccination and the prothrombotic immune thrombocytopenia reported here, caution is required when drawing conclusions about the safety of AZD1222 in the general population. There is a high level of uncertainty in estimating the incidence of this complication. EMA's safety committee PRAC had 62 and 24 cases of CVST and SVT, respectively, reported to its drug safety database as of 22 nd Mar 2021. Most cases were from the UK, where around 25 million people received the vaccine at this time. 14 The risks of COVID-19, including its own risk of thromboembolic complications, appear to be greater than the unexpected thromboembolic risk of the vaccine suggested by this and other emerging reports. [15] [16] [17] Table 1 Baseline characteristics, thromboembolic events, treatment overview and outcome What scientists do and don't know about the Oxford-AstraZeneca COVID vaccine Immunotherapy for COVID-19: Evolving treatment of viral infection and associated adverse immunological reactions Safety and efficacy of the ChAdOx1 nCoV-19 vaccine (AZD1222) against SARS-CoV-2: an interim analysis of four randomised controlled trials in Brazil, South Africa, and the UK Autoimmune heparin-induced thrombocytopenia American Society of Hematology 2018 guidelines for management of venous thromboembolism: heparin-induced thrombocytopenia Autoimmune heparininduced thrombocytopenia and venous limb gangrene after aortic dissection repair: in vitro and in vivo effects of intravenous immunoglobulin Intravenous Immune Globulin (IVIG) for Treatment of Autoimmune Heparin-Induced Thrombocytopenia: A Systematic Review High-dose intravenous immunoglobulin for the treatment and prevention of heparin-induced thrombocytopenia: a review Polyphosphate/platelet factor 4 complexes can mediate heparin-independent platelet activation in heparin-induced thrombocytopenia Complement in hemolytic anemia Thrombosis and Thrombocytopenia after ChAdOx1 nCoV-19 Vaccination Thrombotic Thrombocytopenia after ChAdOx1 nCov-19 Vaccination Pathologic Antibodies to Platelet Factor 4 after ChAdOx1 nCoV-19 Vaccination Author Contributions: AT and AG wrote the paper. AT, AG, JKK and FD analyzed clinical data. UJS, AC, SW, and RB performed laboratory analysis. FD performed imaging analysis. Disclosures: No conflicts of interest to disclose. Data sharing: For original data