key: cord-1042950-cdl3ijzq authors: Ramlal, Preshod S.; Lin, Johnson; Buckley, Christopher A.; Stenström, Thor Axel; Amoah, Isaac D. title: An assessment of the health risks associated with shared sanitation: a case study of the community ablution blocks in Durban, South Africa date: 2022-02-10 journal: Environ Monit Assess DOI: 10.1007/s10661-022-09815-x sha: e082a94a3056ca6f2e31c6e7c425a6a7eca1f784 doc_id: 1042950 cord_uid: cdl3ijzq Shared sanitation facilities have been hailed as an innovative approach to solve the challenge with sanitation access. However, these facilities may act as hotspots for disease transmission due to unhygienic conditions. In this study we used quantitative (based on Escherichia coli contamination) techniques to assess the health risks associated with the use of community ablution blocks (CABs). The most contaminated surfaces were the cistern handle (5.7 Log(10) cfu/cm(2)) and internal pull latch (5.8 Log(10) cfu/cm(2)). Based on the E. coli contamination, at least two people out of 100 CAB users might be potentially infected when they touch “hot” surfaces. These risks were modelled assuming transfer of potentially pathogenic E. coli from these surfaces to the mouth. The incorporation of risk-reduction measures, such as wiping of these surfaces or washing of hands, could potentially result in significant reduction of infection risks. The most significant risk-reduction intervention was determined to be wiping of the contact surfaces, especially twice prior to contact. A combination of risk-reduction interventions could further reduce the risks. This study shows that contamination of contact surfaces within shared CABs could lead to increased risks of infections, requiring measures aimed at reducing the associated risks. The risk assessment framework used in this study could therefore be applied in similar settings to estimate associated health risks with the use of such facilities. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10661-022-09815-x. Shared sanitation can play a critical role in achieving sanitation coverage (Garn et al., 2017; Pickering et al., 2015) . For many people living in densely populated Abstract Shared sanitation facilities have been hailed as an innovative approach to solve the challenge with sanitation access. However, these facilities may act as hotspots for disease transmission due to unhygienic conditions. In this study we used quantitative (based on Escherichia coli contamination) techniques to assess the health risks associated with the use of community ablution blocks (CABs). The most contaminated surfaces were the cistern handle (5.7 Log 10 urban areas, such as informal settlements, the alternative to open defecation is the use of shared sanitation (Busquet, 2015) . However, despite the increase in the use of shared sanitation, there is divided opinion of its appropriateness. While some researchers contend that shared or public toilets (as opposed to individual household toilets) are the best option for densely populated urban slums due to space constraints (Katukiza et al., 2012; Schouten & Mathenge, 2010) , others have shown that shared sanitation may be a contributory factor to increased frequency of infections (Fenn et al., 2012; Heijnen et al., 2014; Patil et al., 2014; Pickering et al., 2015) . This relates to increased risk of diarrhoea, soil-transmitted helminths (STHs), and trachoma (Guerrant et al., 2013) due to direct contamination of contact surfaces with pathogens. Airborne dissemination may also occur, where viruses and bacteria have been found on bathroom surfaces, during toilet flushing (Barker & Bloomfield, 2000; Gerhardts et al., 2012) . The ambient conditions in the sanitation environment are ideal for microorganism survival and proliferation, thereby increasing faecal-oral exposure (Kagan et al., 2002; Kay et al., 2006) . Survival of microorganisms on surfaces such as plastics and metals has been reported extensively (Alsallaiy et al., 2016; Barker & Bloomfield, 2000; Curtis et al., 2003; Neely & Maley, 2000) . Furthermore, contamination of contact surfaces within shared sanitation facilities could be more frequent, with higher microorganism concentrations, than single-household facilities due to higher user numbers and frequency. This has led to several reports of shared sanitation acting as hotspots for diarrhoeal diseases (Baker et al., 2016; Crocker & Bartram, 2016; Rah et al., 2015; Ramlal et al., 2019) . Disease transmission in sanitation facilities could occur either through toilets-to-hands-to-mouth contact or from contaminated hands to surfaces (Barker & Bloomfield, 2000; Curtis et al., 2003) . Private toilets will not always be cleaner than shared facilities (Ahmed et al., 2012) but the number of users may play a significant role in the cleanliness of these facilities (Günther et al., 2012) . When the number of households were below 4 per facility, the cleanliness of shared facilities was comparable to private ones (80% clean), while the sharing of the facilities by more than 10 households resulted in 40% drop in cleanliness (Günther et al., 2012) . In most settings where shared sanitation is practiced, the responsibility of cleaning is shared by the users or volunteers (Kwiringira, 2017) . This has been reported to result in apathy towards the hygienic maintenance of these facilities (Kwiringira, 2017; Tumwebaze & Mosler, 2015) . Therefore, the employment of caretakers, living in the communities where shared sanitation facilities are located, like the community ablution blocks (CABs) in the informal settlements of the eThekwini Municipality, has been recommended as an alternative. This study present the assessment of the health risks associated with the use of shared sanitation facilities, such as the CABs. The quantitative approach adopted in this study presents the assessment of potential health and safety risks associated with the use of shared sanitation facilities. This study aims to make a significant contribution by presenting techniques that can be used to estimate health risks associated with shared sanitation and possible mitigation measures that can be implemented to reduce the risks of infections. CABs located in two informal settlements in the eThekwini Municipality (Durban) of South Africa were selected for this study. Male and female CABs within these settlements were chosen and the health risks associated with its usage were determined based on the concentration of Escherichia coli on key contact surfaces within the CABs. The selected type of contact surfaces was based on recent studies in the field (Bohnert et al., 2016; Mpotane et al., 2013) . The contact surfaces selected included cistern handle, toilet seat, floor surface in front of the toilet, internal pull latch of cubicle door, external door handle of cubicle, tap handle in shower cubicle, internal common floor surface, and tap in wash hand basin (Fig. 1) . These contact surfaces were chosen based on recommendations made in studies by Mpotane et al. (2013) and Bohnert et al. (2016) . Samples were taken with the LABOCARE ™ sterile swab sticks, with transport media (Amies), following the protocol 1 3 Vol.: (0123456789) proposed by Park et al. (2017) . One cubicle each in the male and female CAB toilets was sampled on three different occasions over a period of one month. Aliquots of 0.1 mL of each sample were spread plated on agar plates containing specific media for E. coli (Chromocult media) after a serial of 10 −4 to 10 −10 dilutions. The plate lids were slightly left open after spread plating for 2-3 min to allow for sample absorption. Incubation was made at 37 °C for 24 h. The area swabbed for each surface is presented in Table S1 (Appendix I) and used to calculate the concentration of E. coli per cm 2 of surface area. Ten colonies per sampling site from the Chromocult agar plates were selected based on morphological characteristics and colour (Lange et al., 2013) . These were confirmed biochemically using the IMVic test (Lupindu, 2017) and polymerase chain reaction (PCR) (Abid & AL-zuwainy, 2015) where the uidA gene was used as the marker for the confirmation of E. coli isolates. The primers used are presented in Table S2 (Appendix I). For quality control, each analysis had an additional control, swab sticks taken through the sample analysis process but without actual swabbing. This was intended to ensuring that there was no contamination during the sample processing. The risk of infection was calculated using the quantitative microbial risks assessment (QMRA) approach. This is an approach that combines mathematical equations with knowledge and information on microbial ecology, disease epidemiology, and transmission. This tool has been recommended as a useful technique in assessing the risks associated with bioaerosols, drinking water, reclaimed water, and irrigation water (Carducci et al., 2016; Ezzat, 2020; Girardi et al., 2019; Gularte et al., 2019; Petterson & Ashbolt, 2016) . It therefore could be adopted to provide an assessment approach for the use of shared toilets, such as the CABs. QMRA consists of four interrelated steps: (a) hazard identification; (b) exposure assessment; (c) dose-response assessment, and (d) risk characterization . The hazard of choice for this study was pathogenic E. coli. E. coli isolated from environmental samples could include non-pathogenic environmental strains and the mean E. coli counts per cm 2 would therefore be relatively higher than the pathogenic strains. Therefore, the risk of infection was calculated assuming that 8% of average E. coli counts are pathogenic (George et al., 2013; Howard et al., 2006; Machdar et al., 2013) . The pathogenic E. coli concentrations were then used as doses that were incorporated into the QMRA at the dose-response modelling stage to ascertain the risks. The exposure scenarios used in the health risk assessment were contact with contaminated surfaces within the CABs. The frequency of exposure to these contaminated surfaces was determined based on the frequency of use of the CABs as provided by the respondents during this study. The exposure assessment framework is presented in Fig. 2 . Several dose-response models have been developed for the estimation of risk posed by exposure to pathogenic E. coli. In this study, the beta-Poisson dose-response model was used . The beta-Poisson model is defined by the following equation: With p(d) being the risk of infection and "d" the total concentration of pathogenic E. coli ingested . N 50 is the median infection dose representing the number of organisms that will infect 50% of the exposed population and α the dimensionless infectivity constant. The input values for N 50 and α are presented in Table 1 . The dose of pathogenic E. coli ingested was calculated based on the concentration of E. coli measured on the contact surfaces. This considered that about 8% of total E. coli are pathogenic. Probability distribution functions (PDFs) were fitted to the concentration of pathogenic E. coli using @Risk (Palisade Inc., USA) with the best distribution selected based on the Akaike information criterion (AIC). The dose of E. coli ingested via the framework presented in Fig. 2 was then determined by accounting for the fraction of the bacterial load on the surfaces that will be transferred to the hands and the fraction on these bacteria that will eventually be transferred to the mouth/lips. The transfer efficiency from contact surfaces to hands and hands to mouth/lips was modelled using the input values presented in Table 1 . In the risk characterization, all the outcomes of the hazard identification, exposure assessment, and dose-response assessment were combined to characterize the infection risks for exposed individuals. The risk of infection associated with multiple exposures [P1(A)] was determined using the following formula: is the risk of infection from a single exposure to a dose d of the pathogen, calculated using the beta-Poisson dose-response model presented above; and n being the number of times/days of exposure to the single dose d (Sakaji & Funamizu, 1998) . Two different multiple exposure scenarios were considered, daily and yearly exposures. To determine the risks of infection from multiple exposures within a day, a user frequency survey was conducted as part of a larger household questionnaire. Inhabitants of the two settlements were asked how many times they used these CABs within a day. This gives information on the number of times the users could potentially be exposed to the pathogen. This survey led to modelling the exposures per day with a uniform distribution of a minimum of 1 and maximum of 2 times per day. The yearly exposure risks were modelled assuming that the CABs are used every day of the year; therefore, an n value of 365 was used to calculate the yearly risks. The outcome of the daily risks was used as input for the P(d) in this case to determine the yearly risks. The potential reduction in risks of infection after implementation of risks reduction measures was also determined. Five risk-reduction interventions were considered in this study; these can be further grouped into three categories: wiping of contact surfaces, washing of hands (for at least 20 s), and a combination of these interventions. The various interventions considered were the following: 1. Wiping of contact surfaces once: According to Tuladhar et al. (2012) a simple wipe of surfaces with soap may lead to 1 log 10 reduction in bacterial concentration. 2. Wiping of contact surfaces twice: A second wipe could potentially further reduce the bacterial concentration by 1-3 log 10 . 3. Hand washing with soap: Jensen et al. (2015) reported that washing of hands with soap could achieve a 1.7 (± 0.8) log 10 reduction of pathogens. 4. Hand washing without soap: Washing without the use of soap could potentially result in 1.0 (± 0.4) log 10 reduction. 5. Combination of risk-reduction interventions: The combined effect of wiping of the contact surfaces once and washing of hands without soap was also modelled. These two risks reduction measures were chosen based on the interventions that require the least effort and resources. The input values used for modelling the risks reduction are presented in Table 1 . All risk models were subjected to Monte Carlo simulations of 10,000 iterations for the probability of infections. These models were constructed using the @Risk 7.5 (Palisade Corporation, USA) software add-on to Excel (Microsoft Cooperation, USA). Comparison of the concentration of E. coli on the various surfaces and the associated risk was performed with the Kruskal-Wallis test followed by Dunn's post-test at a 95% confidence interval (Dinno, 2015) . Determination of the statistical significance of the risk-reduction measures was performed with the Mann-Whitney U test. All these comparative statistical analyses were performed using GraphPad Prism (version 7). Quantitative assessment of risks from contact with surfaces within the CABs E. coli concentration on contact surfaces in the CABs E. coli concentration on the contact surfaces varied, although not significantly. Irrespective of the study location, the highest concentration of E. coli was detected on contact surfaces within the female toilets (Fig. 3) . The highest concentration of E. coli was on the cistern handle (6.01 log 10 cfu/cm 2 ), floor surface in front of toilet (6.23 log 10 cfu/cm 2 ), and tap handle (6.25 log 10 cfu/cm 2 ), all within female toilets (Fig. S1, Appendix I) . The difference between the male and female toilets in relation to the concentration of E. coli was statistically significant (p-value ≤ 0.05), on the following surfaces irrespective of sampling location; cistern handle, floor surface in front of toilet, and tap handle in wash basin. However, in some instances the statistically significant differences between the female and male toilets were only observed in one settlement (sampling location) (Fig. 3) . Comparing the different surfaces irrespective of gender, using the toilet and location, the highest mean concentration of E. coli was detected on the cistern handle (5.7 log 10 cfu/cm 2 ), internal pull latch (5.8 log 10 cfu/cm 2 ), external door handle (5.7 log 10 cfu/ cm 2 ), and tap handle in shower cubicle (5.7 log 10 cfu/ cm 2 ) (Fig. 3) . The Mann-Whitney tests showed a statistically significant difference (p-value ≤ 0.05) in E. coli concentrations between the cistern handle and Sampling points E. coli concentration (Log10 cfu/cm 2 ) Fig. 3 Concentration of E. coli on key contact surfaces in community ablution blocks (CABs) within the two settlements toilet seat, the toilet seat and internal pull latch, and between the toilet seat and tap handle in shower. Potential risks of infection with pathogenic E. coli on the contact surfaces before and after incorporation of risk-reduction measures The potential risks of infection with pathogenic E. coli based on the concentration on these contact surfaces varied in a similar fashion to the variation in the E. coli concentration measured and presented in Fig. 3 . Table 2 presents the calculated median risks of infection. Briefly, considering only daily risks, at least two people out of 100 users of the CABs may be infected when they touch surfaces such as the cistern handle, internal pull latch, external door handle, and the tap handles in both the shower and wash basin (10 −2 ) ( Table 2) . However, the highest risk of infection was determined to be contact with the internal pull latch (2.5 × 10 −2 ). Based on the earlier user survey conducted in the study areas, it was observed that the populations within these informal settlements use the CABs up to twice a day. We modelled the frequency of exposure in a day and assessed the risks thereof (daily risks). The daily risks of infection were higher than the one-time exposure risks reported; for instance, the risks of infection after contact with the internal pull latch increased to almost four out of 100 people exposed being infected, compared to the almost three out of 100 for one-time exposure. This increase in risk of infection was also observed for contact with the rest of the other contact surfaces (Table 2) . Multiple exposures over the course of the year may also lead to a statistically significant increase in the risks of infection with pathogenic E. coli. As shown in Table 2 , yearly exposure may result in almost every person who touches these surfaces been infected. This is due to the measured risks of either 1 or 9.9 × 10 −1 per person per year; with the exception of the floor surface in front of the toilet cubicle, which had a risk of infection of 8.6 × 10 −1 (± 6.1 × 10 −3 ) per person per year. Potential risk reduction based on hypothetical risk-reduction measures Implementation of risk-reduction measures, such as cleaning of the surfaces and washing of hands with and without soap, could potentialy reduce the risks as calculated. For instance, wiping of the internal pull latch surfaces could potentially lead to risk estimates of 2.2 × 10 −2 (± 3.1 × 10 −4 ), compared to 2.5 × 10 −2 (± 3.0 × 10 −4 ) for the uncleaned surfaces. Wiping of the surfaces twice reduced the risks further but not significantly. Furthermore, washing of hands without soap reduced the risks to 2.4 × 10 −2 (± 3.0 × 10 −4 ) after contact with the internal pull latch. Washing of hands without soap also reduces the risks as well (Fig. 4) . Comparatively, wiping the surface twice achieves the highest reduction of risks among the four singular risk interventions modelled. Wiping the surface once achieves similar risk reduction compared to wiping the surfaces twice. Combining one wipe of the contact surfaces with hand washing without soap reduces the risks further; however, these risk estimates are not significantly lower than the risks when the surfaces are cleaned (Fig. 4) . Detailed information on the calculated risks are presented in Appendix I (Table S3 ). The detection of E. coli on almost all key contact surfaces in our study shows the potential for these surfaces 9.9 × 10 −1 (± 7.3 × 10 −3 ) 8.6 × 10 −1 (± 6.1 × 10 −3 ) 1 (± 4.8 × 10 −4 ) 9.9 × 10 −1 (± 3.1 × 10 −3 ) 1 (± 3.8 × 10 −3 ) 9.9 × 10 −1 (± 5.4 × 10 −3 ) 9.9 × 10 −1 (± 1.8 × 10 −3 ) to act as possible avenues or routes of pathogen transmission. The observation that the cistern handle, floor, latch of toilet, door handle, and tap handle were the most contaminated surfaces corroborates with other studies (Abiose, 2019; De Alwis et al., 2012; Fankem et al., 2006; Flores et al., 2011; McGinnis et al., 2019; Sabra, 2013; Verani et al., 2014) . For instance, the study by Fankem et al. (2006) observed that the most contaminated surfaces in public toilet facilities found in airports, bus terminals, and universities were toilet seats, sinks, floors, and napkin dispensers. However, that study represented different physical environments, where the prevalence of contamination can be expected to be much lower (3-21%) compared to the findings in this study where all surfaces were contaminated. Furthermore, the toilet facilities in their study were in areas that perhaps had lesser user numbers or frequency of use compared to the CABs located in the informal settlements. In our study area, the CABs serve as the only source of sanitation for the inhabitants in these settlements. Sabra (2013) reported higher occurrence of contamination of contact surfaces within female public toilets, like the findings in our study (100%). They also demonstrated that over 91% of toilet handles were contaminated. Several reasons could account for the contamination. These include direct deposition of faeces on these surfaces, unclean hands and soil. For instance, surfaces such as the cistern handle, the tap handle, and latch of the toilet door could have been contaminated through unclean hands. A study by De Alwis et al. (2012) observed that ntamination of door handles in male toilets were highly contaminated compared to female toilets. This was followed by a survey of the users, who reported that over 50% of the males using these toilets did not wash their hands. Therefore, unclean hands could have accounted for the high contamination rate of regularly touched surfaces. The contamination of the toilet seats and floors next to the toilets could be due to the direct deposition or aerosolization of faecal matter. Flushing of toilets has been reported to play a role in toilet seat contamination due to the generation of droplets or aerosols that may contain some faecal matter (Flores et al., 2011) . Studies have shown that droplets or aerosols generated after multiple flushing could still contain bacteria, although in reduced concentrations. For instance, Johnson et al. (2017) reported a 3 log 10 reduction in bacterial indicators in the bowl water after one flush, 1-2 log 10 after two flushes, and thereafter, less than 1 log 10 reduction. These reports therefore support our hypothesis that the contamination of the toilet seats is primarily due to the presence of E. coli in faecal matter that is deposited either directly on these toilet seats or due to droplets or aerosols generated during flushing. One of the other most contaminated surfaces was the floor, which could be attributed to soil from footwear (Flores et al., 2011) . The presence of these bacteria in the soil could be from faecal contamination or normal microflora. During the study we observed that children were playing on the floor within these toilet cubicles, thus highlighting a significant health risk. In addition to soil being the main source of floor contamination, it could also account for contamination of the cistern handles, in addition to unclean hands (Flores et al., 2011) . This conclusion was based on the observation that some people used their feet for flushing of the toilets and the presence of a similar bacterial community on the toilet floors and the cistern handles. It has also been reported that some persons within the study area wipe faeces with their hands and smear these faeces on walls, either due to habit or religious reasons. This was corroborated by our findings, where faecal contamination on the CAB walls was commonly observed as shown in results above. This practice could have contributed to the contamination of the other contact surfaces. The detection of E. coli on the various contact surfaces highlights the potential risks of infection. These surfaces could, therefore, be harbouring large concentration of other potentially pathogenic microbes, as inferred from the concentration of E. coli on these surfaces. The calculated risk due to contact with these surfaces is high. For instance, almost 3 people out of 100 exposed to the internal pull latch of the toilet door could be infected. This risk is for one-time use, with increasing risks when one considers the multiple uses during a day and the reliance on these facilities throughout the year. Although the risks were lower for the other surfaces, these were not statistically significant except when compared to the internal common floor. The risk of infection based on the bacterial concentration on the toilet floor in the common area was 9.7 × 10 −3 (± 1.8 × 10 −3 ) per person. This is much lower than the risks associated with the other seven surfaces, mainly due to the likelihood of such exposure occurring. As presented in Fig. 2 , hand contact was assumed as the main route of exposure; therefore, the possibility that users of the CABs will touch the toilet floor is lesser compared to touching the other surfaces. Therefore, the potential risk calculated is less than the other contact surfaces. None the less when one considers the fact that on the average uses of the CABs will result in touching more than one surface then the risks presented in Table 1 could be higher. For instance, on average all users of the CABs will be exposed to the external door handle of the toilet cubicle, the toilet seat, and cistern handle. If they wash their hands, the tap handle is included. Therefore, the combined risks from exposure to these multiple surfaces will be higher. The risk estimates calculated in this study are higher than the estimates reported by Ryan et al. (2014) for touch with surfaces contaminated with E. coli 0147:H7. In that study a single touch risks of 1.5 × 10 −4 was reported. The risk estimates in this study are considerably higher than the tolerable risk figure of 1 in 1 million (10 −6 ) recommended by Ryan et al. (2014) for touch with contaminated surfaces. This calls for the implementation of risk-reduction measures. The risks of infection when simple mitigation measures such as thorough cleaning of the surfaces and washing of hands with and without soap are implemented were calculated to be lower. A simple wipe of surfaces with soap could lead to about 1 log 10 bacterial reduction on surfaces (Tuladhar et al., 2012) . A second wipe achieved a further 1-3 log 10 reduction. Therefore, regular wiping on these surfaces by either the users or caretakers could potentially reduce the contaminations further. This will subsequently lead to lower risks of infections. A further reduction in the risks is achievable if the users of these facilities washed their hands either with or without soap. Incorporating these possible risk-reduction strategies resulted in a reduced risk as presented in Fig. 4 . The most important or significant risk-reduction measure among those considered in this study is wiping the contact surfaces especially twice. For instance, the risk of infection due to contact with the internal pull latch of the toilet door after wiping once was reduced to about two out of a 100 people infected (2.2 × 10 −2 (± 3.1 × 10 −4 )) per person per one-time exposure (Fig. 4; Table S3 ). Although hand washing was observed to possibly lead to risk reduction, the estimates achieved in this study indicate that this is not the most significant intervention. The reduction in pathogen concentration when hands are washed with soap was assumed to be only 1.7 (± 0.8) log 10 (Jensen et al., 2015) . This therefore could be the main reason for the little effect of hand washing on its own. It must also be noted that none of the risk-reduction options modelled in this study could reduce the risks to the tolerable/acceptable risks estimate of (10 −6 ) recommended by Ryan et al. (2014) . The detection of E. coli, an indicator for faecal pollution, and the associated risks estimated shows the potential for other pathogens on these surfaces. E. coli is widely used and accepted as a faecal indicator organism (McLellan & Eren, 2014; Wen et al., 2020) . However, the utility of E. coli as an indicator for determination of risk of infection with other pathogens has been questioned due to reported differences in survival and infectivity. For instance, most STHs require some period of latency before becoming infectious. Ascaris spp. ova/eggs, as an example, require a latency period of 2-4 weeks at temperatures between 15 and 38 °C before becoming infectious (Bogitsh et al., 2012) . Therefore, although the detection of E. coli on the contact surfaces gives an indication of the potential risks of infection for other faecal pathogens, it must be noted that it may either over-or underestimate the risks. This study shows that contamination of key contact surfaces within shared sanitation facilities is a common occurrence. Contamination could be due to several factors pertaining to hygiene practices and general habits of the users of these facilities including direct deposition, aerosol generation from toilet flushing, unclean hands, and soil from footwear. Contamination of key contact surfaces poses potentially higher risks of infection, with almost everyone at risk of infection over the course of a year due to reliance on these facilities. The incorporation of risk-reduction strategies, such as wiping of surfaces and washing of hands, has the potential to reduce infection risks; however, the use of these CABs still poses significant health risks. Furthermore, this study has shown that QMRA can be adapted to present a powerful tool for measuring the potential risks associated with the use of shared sanitation facilities. Therefore, to reduce the risks associated with the use of CABs within the study area, the following recommendations are made: 1. Effective cleaning of key contact surfaces: Cleaning of key contact surfaces could potentially reduce the concentration of potential pathogens and associated risks of infection. 2. Re-training of caretakers: To achieve effective cleaning, re-training of caretakers is suggested. Focus on the importance of cleaning key surfaces that are regularly touched by users should be considered. 3. Improved hygiene of users: There is the need for public education among users of the CABs to encourage hygiene practices such as washing of hands, with or without soap, to reduce contamination of other surfaces. The provision of soap for handwashing could potentially reduce the prevalence of contamination even further. 4. Provision of refuse bins at each CAB: Regular waste collection will deter littering both internally and externally of the facility. 5. Community education: The contamination levels within the CABs could also be considerably reduced if the community members are educated on the consequences of contamination of these areas and how to ensure proper hygiene practices. This could be in the form or posters and audiovisual materials. Despite the significant findings made in this study, some limitations were identified. These limitations include the following: 1. Sampling frequency and sampling points: This study was impacted by the COVID-19 pandemic, limiting the ability to sample more frequently and include more sampling points. Additionally, lockdown restrictions were imposed in the city of Durban, which affected the use of the CABs within the study locations. These limitations have the potential to affect the concentration of E. coli, with subsequent impact of the risk estimations. 2. Probabilistic risk estimations: The practice of estimating risks based on probability introduces a level of uncertainty which could be a limitation with respect to the reliability of the data. In this study, variability in E. coli concentrations was accounted for using Monte Carlo simulations with up to 10,000 iterations. However, it must be acknowledged that the use of probabilistic risk estimations could be a limitation. 3. Pathogenic E. coli concentration: In this study the concentration of pathogenic E. coli was estimated based on the percentage of total E. coli that is reported to be pathogenic. This could lead to either under-or overestimation of the concentration of pathogenic E. coli in the study area, which could have effect of the level of risks estimated. The findings of this study have implications for future practice in relation to sanitation delivery. Evaluation of sanitation facilities has largely focused on the use of these facilities and their physical state. However, this study presented an approach that can be used to estimate risks associated with the use of these facilities. This approach could be added to the evaluation of sanitation facilities to provide an input on the potential risks. This would be very instrumental in ensuring that sanitation facilities do not become hotspots for disease transmission. 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