key: cord-1049002-4iqcg7ki authors: Muhammad, Zeinab A.; Farghaly, Thoraya A.; Althagafi, Ismail; Al‐Hussain, Sami A.; Zaki, Magdi E. A.; Harras, Marwa F. title: Synthesis of antimicrobial azoloazines and molecular docking for inhibiting COVID‐19 date: 2021-03-21 journal: J Heterocycl Chem DOI: 10.1002/jhet.4257 sha: 82eddb78635479eb1ff126f8ffd0492ffbec7c13 doc_id: 1049002 cord_uid: 4iqcg7ki Diverse new azoloazines were synthesized from the reaction of fluorinated hydrazonoyl chlorides with heterocyclic thiones, 1,8‐diaminonaphthalene, ketene aminal derivatives, and 4‐amino‐5‐triflouromethyl‐1,2,4‐triazole‐2‐thiol. The mechanistic pathways and the structures of all synthesized derivatives were discussed and assured based on the available spectral data. The synthesized azoloazine derivatives were evaluated for their antifungal and antibacterial activities through zone of inhibition measurement. The results revealed promising antifungal activities for compounds 4, 5, 17a,b, 19, and 25 against the pathogenic fungal strains used; Aspergillus flavus and Candida albicans compared to ketoconazole. In addition, compounds 4, 5, 19, and 25 showed moderate antibacterial activities against most tested bacterial strains. Molecular docking studies of the promising compounds were carried out on leucyl‐tRNA synthetase active site of Candida albicans, suggesting good binding in the active site forming stable complexes. Moreover, docking of the synthesized compounds was performed on the active site of SARS‐CoV‐2 3CLpro to predict their potential as a hopeful anti‐COVID and to investigate their binding pattern. Multidrug resistance of several strains of microorganisms, due to antibiotics misuse, has been emerged as one of the main serious problems. [1] Thus, novel antimicrobial drugs with new targets should be developed to overcome the increased occurrence of microbial resistance. Additionally, the increased incidences of fungal infections in an invasive form, particularly in immunecompromised patients, have led to many deaths globally. [2] Accordingly, the need for novel antifungal agents is also an increasing concern. The leucyl-tRNA synthetase is one of the highly validated targets for antimicrobial agents. It plays a key role in the tRNA aminoacylation and synthesis of the microbial proteins. The effective antifungal, 5-fluoro-1,3-dihydro-1-hydroxy-2,1-benzoxaborole (AN2690), was reported to inhibit leucyl-tRNA synthetase by trapping the editing site of the enzyme. [3] [4] [5] From another side, triazolopyrimidines showed in several reports different types of potent biological activities including antimalarial, [6] anti-Alzheimer, [7] antihypertensive, [8] leishmanicidal cardiac stimulant, [9] anti-HBV, [10] antifungal, [11] anticancer, [12] antimicrobial, [11] and herbicidal activities. [13] Pogaku and coworkers synthesized new derivatives of triazolopyrimidine, which revealed α-glucosidase inhibitor activity. [14] In addition, the presence of fluoride atom on the skeleton of heterocyclic drugs improves their activity. [15, 16] Recently, the widespread of COVID-19, caused by the new coronavirus 2 (SARS-CoV-2), all over the world has emerged as a serious concern to public health. [17] Up to now, millions of infections and deaths have been recorded worldwide. In this consequence, there is a critical need for new antiviral agents targeting the virus life cycle, membrane fusion, or the virus replication. [18] The main protease affords a greatly validated antiviral target for the design and discovery of inhibitors. SARS-CoV-2 main protease (Mpro) is a vital protein in the lifecycle of SARS-CoV, making it an ideal target to anti-COVID-19 agents. [19] [20] [21] All previous facts, together with our experiences in the field of synthesizing bioactive heterocyclic ring systems, [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] lead us in this context to synthesize new fluorinated derivatives of triazolopyrimidines pyridopyrimidine, naphthodiazipene, triazolothiadiazine, and triazolotetrazine. The newly synthesized azoloazine derivatives were evaluated for their antimicrobial activities against different antibacterial and antifungal strains. Moreover, molecular docking analysis was done to predict the possible binding interactions between derivatives with promising activities and the editing domain of leucyl-tRNA synthetase. Furthermore, a docking study of the synthesized compounds was used to demonstrate the probability of these compounds to inhibit SARS-CoV-2 main protease. Several reviews and our previous articles [33] [34] [35] [36] [37] indicated the efficacy of the various derivatives of hydrazonoyl halides in the synthesis of bioactive heterocyclic systems. Herein, we used two fluorinated hydrazonoyl chlorides 4 and 5 to synthesis fused azole derivatives. Scheme 1 indicates the synthetic procedure of the two reactive fluorinated hydrazonoyl chlorides as described previously in our lab. [38] Firstly, the two hydrazonoyl chlorides 4 and 5 were reacted with quinazoline-2-thione derivative 6 in dioxane/Et 3 N as indicated in the experimental part to afford [1, 2, 4] triazolo [3,4-b] quinazoline derivatives 9a,b. The structure of the latter products 9a,b were assured based on the data obtained from their spectral data and their comparison with the literature reports. The disappearance of the absorption band for the carbonyl group from the IR spectra of the products 9a,b proved the cyclized products of such reactions. Moreover, 1 H NMR of the two derivatives 9a,b were free from any NH signals and showed the expected aliphatic and aromatic protons' signals. The suggested pathway for the formation of [1, 2, 4] triazolo [3,4-b] quinazoline derivatives 9a,b is outlined in Scheme 2, starting from the formation substituted intermediate 7 followed by the smiles rearrangement [39] to give the intermediate 8. Target triazolopyrimidines 13a-d have been synthesized through the same pathway as compounds 9a,b (Scheme 3). The stoichiometric quantities of 6-substituted pyrimidine thione derivatives 10a,b and hydrazonoyl chlorides 4 or 5 were refluxed in a suitable amount of dioxane in the presence of Et 3 N. Good yields (69%-81%) with high degree of purity of the products 13a-d were obtained. The structure of the isolated triazolopyrimidines 13a-d was confirmed based on the results obtained from their spectroscopic analyzes. We selected two charts of spectra one 1 H NMR of compound 13b ( Figure 1 ) and one 13 C NMR of derivative 13d ( Figure 2 ) to discuss their structures. 1 H NMR of triazolopyrimidine derivative 13b showed three signals for 3CH 3 groups with the expected splitting and one quartet signal for CH 2 of ester-CH 2 CH 3 group, in addition to the presence of the pyrimidine-5-H as well as the three signals for aromatic-H(s). 13 C NMR is an excellent tool to prove the structure of the triazolopyrimidine derivatives 13a-d. As an example for the prepared series 13a-d, 13 C NMR of derivative 13d ( Figure 2 ) showed six splitted carbons due to the coupling between fluoride and carbon atoms at δ = 14.3 (CH 3 , dd, 3 JCF, 3 Hz), 108.0 (dd, 2 JCF, 28 Hz), 116.8 (dd, 3 JCF, 3 Hz), 124.2 (dd, 2 JCF, 17 Hz), 132.0 (dd, 3 JCF, 6 Hz), and 159.4 (dd, 1 JCF, 243 Hz). [40] Also, the appearance of a carbon signal of the amidic CO at δ = 162.9 (C O) ppm proved the cyclization of compounds 13 on the amidic nitrogen N3 not on the N1 in the pyrimidine ring. [11, 34] Under the same reaction condition, perimidine-2-thione 14 was reacted with each of the fluorinated All the chemical structures of the synthesized products 17a,b, 19, and 21a,b were confirmed based on the corrected data, which were extracted from their spectra (NMR, Mass, IR). For example, the mass spectra of all derivatives 17a,b, 19, and 21a,b showed correct molecular ion peaks at the expected molecular weight for each derivative and all elemental analyses in the expected range as the calculated numbers. Furthermore, the IR spectra of derivatives carbonyl group region (ν = 1630-1800 cm −1 ). However, in the case of compound 28, its IR spectrum has sharp absorption band at ν = 1736 cm −1 for the ester carbonyl group. In addition, the mass spectra for each compound 25 and 28 showed molecular ion peaks at the expected values corresponding to the suggested structures in Scheme 6. Antifungal and antibacterial activities of the target compounds were done at the Regional Center for Mycology and biotechnology (RCMB), Al-Azhar University, Cairo, Egypt. Compounds 4, 5, 9a,b, 13a-d, 17a,b, 19, 21a,b, 25, and 28 were examined for their in vitro antifungal, and antibacterial activities, by inhibition zone (IZ) technique. Two fungi: Aspergillus flavus (A. flavus, RCMB 002008) and Candida albicans (C. albicans, RCMB 005003), three Gram-positive bacteria, Staphylococcus aureus (S. aureus, RCMB 010010), Bacillus subtilis (B. subtilis, RCMB 015), Enterococcus faecalis (E. faecalis, ATCC 29212), and three Gram-negative bacteria: Escherichia coli (E. coli, RCMB 010052), Proteus vulgaris (P. vulgaris, RCMB 004), and Enterobacter cloacae (E. cloacae, RCMB 001), were used for this study compared to ketoconazole and gentamycin as reference antifungal and drugs antibacterial, respectively. The hydrazonoyl chlorides 4 and 5 revealed superior antifungal activities with IZ = 17 and 15 mm against A. flavus and 18 and 20 mm against C. albicans, respectively, compared to ketoconazole (IZ = 17 and 20 mm against A. flavus and C. albicans, respectively). Additionally, the triazolo thiadiazine derivative 25 exhibited potent antifungal activity (IZ = 15 and 18 mm against A. flavus and C. albicans, respectively). Moreover, the triazolopyrimidines 17a,b, and dihydronaphtho diazepin-2 (1H)-one derivative 19 displayed moderate activities with IZ values ranging from 9 to 14 mm. On the other hand, the triazolopyrimidines 9a,b, and 13a-d in addition to compounds 21a,b, and 28 did not show any antifungal activities (Table 1 ). Concerning the antibacterial activities of the synthesized compounds, moderate activities were observed for compounds 4 and 5 against most tested Gram-positive and Gram-negative bacterial strains showing IZ values at 13-25 mm. Regarding triazolopyrimidines 9a,b and 13a-d, no activity was seen against the Gram-positive B. subtilis or Gram-negative E. coli, while mild activities were noticed against P. vulgaris and E. cloacae with the exception of 9a that was inactive against E. cloacae. Furthermore, compound 19 showed moderate antibacterial activities against E. faecalis and P. vulgaris with IZ = 20 mm in comparison to gentamycin (IZ = 26 and 25 mm against E. faecalis and P. vulgaris, respectively). Also, compound 25 revealed moderate activity against P. vulgaris (IZ = 18 mm). The rest of the compounds displayed weak or no antibacterial activities ( Table 2 ). As a general talk, we can conclude that the hydrazonoyl chlorides 4 and 5 showed the best antimicrobial activities among the tested compounds and that cyclization of the hydrazonoyl chloride moiety with different cyclic systems did not improve the activity. Leucyl-tRNA synthetase (LeuRS) is a member of the aminoacyl tRNA synthetases family. It plays a critical role in the synthesis of protein of the microorganism, and thus essential for its survival, making this enzyme an attractive antimicrobial target. [41] Many recent studies in the literature have discussed the significance of aminoacyl tRNA synthetases family in developing antifungal and antibacterial agents. [42] [43] [44] [45] Here, we performed molecular docking study of compounds 4, 5, 17a,b, 19, and 25, with significant antifungal activities, into the editing domain of Candida albicans cytosolic leucyl-tRNA synthetase (PDB code: 2WFG) to analyze their binding mode and affinity using MOE 2014 program. Analysis of the docking simulation results (Table 3 , Figures 3-8 ) revealed good fitting in the active site with docking scores from −5.014 to −6.851 kcal/mol, forming different types of interactions as hydrogen bond, π-cation, π-H, and hydrophobic interactions. All docked compounds formed interaction with the key amino acid residue Leu317 either through hydrogen bond or π-H interaction. The carbonyl group of the hydrazonoyl chlorides 4 and 5 interacted through H-bond with Leu317, while their phenyl moieties interacted through arenecation interactions with Lys483 and Lys407, respectively. Also, the carbonyl group of 17a was responsible for hydrogen bonding with Leu317, and the triazoloperimidine group formed two arene-H interactions with Lys483. On the other hand, triazoloperimidine moiety of compound 17b was oriented in close contact with Leu317 forming arene-H interaction, however the ester oxygen was involved in hydrogen bonding with Lys483. Additional πcation interaction was observed between the triazolo moiety of 17b and Lys483. Regarding the binding mode of the dihydronaphtho diazepin-2(1H)-one derivative 19, arene-H interaction was formed between the diazepine and Leu317. Two hydrogen bonds were also seen, one is formed between the carbonyl oxygen and Thr316 and the second is between the hydrazono nitrogen and Lys483. Furthermore, compound T A B L E 2 Antibacterial activity of the target compounds expressed as mean zones of inhibition (mm) The 3CLpro-2, the main protease of SARS-CoV-2, is vital in the maturation and replication process of the SARS-CoV-2, making it an important target for discovering new anti-coronavirus drugs. [18] The structure of the 3CLprotease enzyme comprises three distinctive domains (Domain I, Domain II, and Domain III) made of 13 β-strands and 9 α-helices. In addition, there is a catalytic dyad in its structure formed of the conserved His41 and Cys145 residues, while the main site for substrate-binding appears as splitting between Domains I and II. [46] To predict the binding mode and affinity of compounds 4, 5, 9a,b, 13a-d, 17a,b, 19, 21a,b, 25, and 28 into the binding site of COVID-19 3CLpro (PDB: 6LU7), molecular docking simulation was performed. As represented in Table 4 , most of the tested compounds fit properly in the COVID-19 3CL protease active site with binding energies ranging from −5.510 to −7.529 kcal/mol. Generally, all of the compounds, with the exception of 9a, formed hydrogen bond interaction with the key amino acid Cys145. Besides, residues Asn142, Gly143, Ser144, His163, His164, and Gln189 were also contributed in hydrogen bonding. While His41, Gly143, Met165, and Gln189 were involved in π-H interactions. Focusing on the mode of binding of the hydrazonoyl chlorides 4 and 5, strong binding with docking score values of −7.158 and −7.529 Kcal/mol, respectively, were observed. As shown in Figures 9 and 10 , hydrogen bond interactions were formed between the hydrazono NH groups of 4/5 and both Cys145 and His164 residues. Another hydrogen bonds were noticed between the ester group of 5 and Asn142. Also, the triazolopyrimidine derivatives 13c and 13d could fit well in the active site (docking score −6.954 and −7.239 Kcal/mol, respectively). One of their triazolo nitrogen atoms formed hydrogen bond with Gly143, while the acetyl/ester carbonyl groups were involved in hydrogen bonding with the important Cys145 residue. Moreover, the phenyl groups of 13c and 13d were found to form H-π interactions with His41 ( Figures 11 and 12) . Concerning the triazolo thiadiazine derivative 25, the hydrazono NH group was responsible for the hydrogen bonding with Cys145 and His164, while the triazolo nitrogen atom formed hydrogen bond with His163. Besides, π-H interaction was seen between the triazolo thiadiazine moiety and Met165 as displayed in Figure 13 . Ultimately, this research article contains valuable diverse azoloazine derivatives that we synthesized from the reaction of two fluorinated hydrazonoyl chlorides with heterocyclic thiones, 1,8-diaminonaphthalene, ketenaminal derivative, and 4-amino-5-triflouromethyl-1,2,4-triazole-2-thiol. The mechanistic pathways and the structures of all synthesized derivatives were discussed and assured based on the available spectral data. The antimicrobial evaluation of the target compounds revealed that the hydrazonoyl chlorides 4 and 5 were the most active as antimicrobials among the tested compounds. Additionally, compounds 17a,b, 19, and 25 Melting points of all new isolated heterocyclic derivatives 9a,b, 13a-d, 17a,b, 19, 21a,b, 25, and 28 were detected on a Gallenkamp device without correction. IR spectra of these derivatives 9a,b, 13a-d, 17a,b, 19, 21a,b, 25, and 28 were determined after converted to disc with potassium bromide using spectrophotometer (Pye-Unicam SP300). Moreover, solution of deuterated DMSO-d 6 was used to determine the spectra of 1 H and 13 C NMR on a Varian Gemini 300 NMR spectrometer (400 MHz for 1 H NMR and 100 MHz for 13 C NMR) and the two chemical shifts values at δ: 2.5 and 3.3 ppm were corresponding to the utilized solvent (DMSOd 6 ). Mass spectra for all synthesized derivatives were recorded on a GCMS-Q1000-EX Shimadzu and GCMS 5988-A HP spectrometers, the ionizing voltage was 70 eV. Elemental analyses of the products were carried out at the Microanalytical Centre of Cairo University, Giza, Egypt. The biological evaluation of the products was carried out at Regional Center for Mycology and Biotechnology at Al-Azhar University, Cairo, Egypt. The two fluorinated hydrazonoyl chlorides were prepared according to our literature report. [38] 3. The synthesized azoloazine derivatives 4, 5, 9a,b, 13a-d, 17a,b, 19, 21a,b, 25, and 28 were assayed in vitro for their antimicrobial activity using the agar diffusion method. [47] The activity of 30 μg/mL concentration of each azoloazine derivatives was investigated separately on each microorganism. The IZD diameter of the mm/mg azoloazine compounds is used as a standard for antimicrobial impact. Initially, using a sterile cotton swab, the microorganisms were spread uniformly on fresh Petri dishes with agar-agar and nutrient agar. One hundred μl of each sample was taken and added to each well (10 mm holes cut in an agar gel, 20 mm away from each other). At a temperature of 36 C, the Petri dishes were incubated for bacteria, and the fungi were incubated at 28 C. After about 24 hours of incubation, areas of inhibition were measured in millimeters. Each test was repeated three times and the average value was recorded. The fungicide amphotericin, gentamicin, and ampicillin were used as reference to evaluate the effectiveness of compounds tested under the same conditions. The results of the antimicrobial and antifungal effects test are summarized in Tables 1 and 2. Molecular docking analysis was done by using MOE-Dock 2014 software. [48] Chemical structures of the tested compounds were sketched by the builder of the MOE and minimized using the program force field MMFF94x. Then, the protein was prepared, hydrogen atoms were added, and undesirable water molecules were removed. After that, docking of the 3D conformers was done; using rescoring 1(London dG) and rescoring 2 (GBVI/WSA dG). "Ligand Interactions" tool was utilized for the visualization of the 2D protein-ligand interactions, showing the different formed interactions. The data that supports the findings of this study are available in the supplementary material of this article ORCID Zeinab A. Muhammad https://orcid.org/0000-0003-1711-8497 Thoraya A. Farghaly https://orcid.org/0000-0002-9620-8548 1054 cm −1 ; 1 H NMR (DMSO-d 6 ): δ 2.28 (s, 3H, CH 3 ), 2.35 (s, 3H, CH 3 ), 2.70 (s, 3H -fluoro-4-methylphenyl)-7-methyl-5-oxo-1,5-dihydro White solid, 69% yield; mp. 170 to 172 C 1120 cm −1 ; 1 H NMR (DMSO-d 6 ): δ 1.35 (t, J = 7 Hz, 3H, CH 3 ), 2.31 (s, 3H, CH 3 ), 2.51 (s, 3H, CH 3 ), 4.46 (q | 3-Acetyl-1-(3-fluoro-4-methylphenyl)-7-phenyl Yellow solid, 77% yield; mp. 210 to 212 C 1109 cm −1 ; 1 H NMR (DMSO-d 6 ): δ 2.20 (s, 3H, CH 3 ), 2.47 (s, 3H, CH 3 ), 6.30 (s, 1H MS m/z (%): 364 (M + +2, 10), 362 (M + , 29) -fluoro-4-methylphenyl)-5-oxo-7-phenyl-1,5-dihydro cm −1 ; 1 H NMR (DMSO-d 6 ): δ 1.38 (t MS m/z (%): 392 (M + , 21) ]perimidin-10-yl)ethan-1-one (17a) Orange solid, 70% yield; mp. 235 to 237 C; IR (KBr): v 3086, 3052 (sp 2 C H), 2974 (sp 3 C H), 1709 (C O), 1643, 1618 (C N) MS m/z (%): 359 (M + +1, 7), 358 (M + , 48) cm −1 ; 1 H NMR (DMSO-d 6 ): δ 1.38 (t, J = 9 Hz, 3H, CH 3 ), 2.26 (s, 3H, CH 3 ), 4.50 (q MS m/z (%): 390 (M + +2, 20), 388 (M + , 35) -Fluoro-4-methylphenyl) hydrazono)-3,4-dihydronaphtho[1,8-ef Brown solid, 73% yield; mp. 155 to 157 C; IR (KBr): ν 3397 (br., 3NH), 3046 (sp 2 C H) cm −1 ; 1 H NMR (DMSO-d 6 ): δ 2.35 (s, 3H MS m/z (%): 336 (M + +2, 43), 334 (M + , 32) -fluoro-4-methylphenyl)hydrazono)-7,9-dihydro-10H-pyrrolo[1,2-a]perimidin-10-one (21a) Orange solid, 70% yield 2NH), 3058 (sp 2 C H), 2922 (sp 3 C H), 1678, 1648 (2C O), 1619 (C N) MS m/z (%): 402 (M + , 39), 400 (M + , 29) -fluoro-4-methylphenyl)hydrazono)-7,9-dihydro-10H-pyrrolo[1,2-a]perimidin-10-one (21b) Orange solid, 76% yield 1692, 1648 (2C O), 1622 (C N) MS m/z (%): 462 (M + , 9 -Fluoro-4-methylphenyl) hydrazono)-6-methyl-3-(trifluoromethyl)-7H MS m/z (%): 360 (M + +2, 34), 358 (M + , 100) -fluoro-4-methylphenyl)-3-(trifluoromethyl)-5,8-dihydro 2982 (sp 3 C H), 1736 (C O), 1626 (C N) MS m/z (%): 374 (M + +2, 31), 372 (M + , 35) SUPPORTING INFORMATION Additional supporting information may be found online in the Supporting Information section at the end of this article. 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