key: cord-1056064-36wqulk8 authors: Parreño, V.; Costantini, V.; Cheetham, S.; Blanco Viera, J.; Saif, L. J.; Fernández, F.; Leoni, L.; Schudel, A. title: First Isolation of Rotavirus Associated with Neonatal Diarrhoea in Guanacos (Lama guanicoe) in the Argentinean Patagonia Region date: 2008-07-07 journal: J Vet Med B Infect Dis Vet Public Health DOI: 10.1111/j.1439-0450.2001.00486.x sha: 7d44260707305e15ac0a35c039db71fe2d17fbd5 doc_id: 1056064 cord_uid: 36wqulk8 Group A rotavirus (RV) and coronavirus (CV) are common viral pathogens associated with neonatal diarrhoea in numerous animal species. The purpose of this work was to investigate the presence of these viral agents in two farm populations of captured guanacos (Lama guanicoe) in the Argentinean Patagonia region, that developed severe diarrhoea outbreaks. Stool and serum samples were analysed for RV and bovine CV antigen and antibody by enzyme‐linked immunosorbent assay. Rotavirus was detected in faeces from two new‐born guanacos with acute diarrhoea, one in each farm. After electrophoretic analysis, each isolated strain, showed a distinctive long dsRNA electropherotype characteristic of group A rotaviruses (4:2:3:2). In addition, 95% (38 of 40) of the sampled animals were positive for RV antibodies, suggesting a high prevalence of RV infection in the populations tested. No evidence of CV circulation by antigen or antibody analysis was observed. To our knowledge, this is the first report of the detection and isolation of RV associated with neonatal diarrhoea in Lama guanicoe. Lama guanicoe commonly known as guanaco is one of the four species of South American camelids that include: llama (Lama glama), alpaca (Lama pacos), guanaco (Lama guanicoe) and vicun Äa (Lama vicugna). Guanaco exist as a wild species in most of the Argentinean arid areas, particularly in the Patagonia region. As there is increasing interest in Camelidae ®bres, in recent years, well-controlled programmes for capture, temporary housing, shearing and release of young guanacos have been implemented. There are relatively few reports concerning viral diseases of South American camelids. In particular, in Lama guanicoe there is only one report in which, sera from 20 free-ranging guanacos from Chubut province, Argentina, were tested for antibodies against bluetongue, bovine respiratory syncytial virus, bovine viral diarrhoea virus, parain¯uenza-3, equine herpesvirus-1, vesicular stomatitis virus and foot and mouth disease virus, and all animals were seronegative for all the antigens tested (Karesh et al., 1998) . However, there is no information regarding the circulation of enteric viral agents such as rotavirus (RV) and coronavirus (CV) among these animals. Rotaviruses are a major cause of neonatal diarrhoea in humans and numerous animal species world-wide (Kapikian and Chanock, 1996) . In Argentina, RV is considered one of the most important causes of diarrhoea in calves (Barrandeguy et al., 1988; Bellinzoni et al., 1989 Bellinzoni et al., , 1990 Costantini et al., 1999) , and its presence has been reported in piglets and foals Parren Äo et al., 1997) . Coronavirus is commonly associated with calf diarrhoea and winter dysentery in adult cattle in countries of the northern hemisphere (Saif, 1990; Clark, 1993) . Although serologic surveys of adult cattle indicate that bovine CV circulates among Argentinean cattle (Panighi, 1990) , its incidence associated with neonatal calf diarrhoea in Argentina is very low (Parren Äo et al., 1996) . Coronaviruses have been detected by electron microscopy in the faeces of llama with diarrhoea (Mattson, 1994) . There are also two previous reports of the detection of antibodies against RV in alpacas in Peru (Rivera et al., 1987) and llamas in Argentina (Puntel et al., 1999) , but to the authors knowledge there have been no reports of the detection or isolation of RV. The aim of this study was to investigate the presence of RV and CV as possible agents associated with severe diarrhoea outbreaks, with high morbidity and mortality, affecting young animals during the calving season of 1998, in two farms dedicated to Lama guanicoe domestication in the Argentinean Patagonia region. This investigation was conducted in two farms working under the permission of the regulatory agency for`controlled capture of young guanacos'. The farms were located 700 km apart, in the Provinces of Rio Negro (farm A) and Chubut (farm B), in the Patagonia region. Young wild guanacos (1 day to 4 months old) were captured, maintained in small yards and fed with bovine milk substitute twice a day. By November/December 1998, outbreaks of severe acute diarrhoea with 100% morbidity and 83% mortality rates were observed in both farms. The affected animals were from 7 to 40 days old, and all developed an acute dark-green diarrhoea, hypothermia (rectal temperature lower than 38°C) and anorexia, followed by dehydration and death in a period of 2±6 days. Initial diagnosis was bacterial diarrhoea, but speci®c antibiotic treatment proved to be ineffective and no reduction in morbidity or mortality rates were observed. However, in the analysed cases, necropsy results indicated the presence of Escherichia coli (in three dead animals in farm A) and Salmonella sp. (in one new-born guanaco in farm B), with septicaemia as the ®nal causes of death. Both farms were sampled approximately 30 days after the peak of the outbreak. A total of 22 faecal and 16 serum samples were collected in farm A and 30 faecal and serum samples were obtained in farm B, belonging to the animal categories described in Table 1 . All faecal samples were initially screened for the presence of RV and bovine coronavirus (BCV) antigen by enzyme-linked immunosorbent assay (ELISA), using the reagents and techniques previously described by Cornaglia et al. (1989) for RV antigen detection; and Smith et al. (1996) for CV antigen detection (source: L. J. Saif, Food Animal Health Research Program, The Ohio State University, Wooster, Ohio, USA). Additionally, both ELISA techniques were adapted for RV and CV antibody detection in guanaco serum samples. Clari®ed supernatants of NCDV-Lincoln BRV or Mebus BCV were used for the antigen-coated wells and mock-infected MA-104 or HRT 18 cell culture control lysates for the control-coated wells (cell source: L.J. Saif). Each guanaco serum sample was assayed in serial Rotavirus antigen was detected in two of 53 faecal samples tested, corresponding to two new-born guanacos at 7 and 2 days of age, showing severe acute diarrhoea at the time of sample collection. The former animal belonged to farm A, was captured 5 days before sampling, and was fed with commercial milk. The latter animal was captured in farm B and died the day after sample collection and necropsy results indicated septicaemia by Salmonella sp. (data not shown). Both animals were negative for serum RV antibodies by ELISA. (Table 1, Fig. 1 ) and showed low levels of total protein in serum determined by a glutaraldehyde test (data not shown). The rest of the young guanacos had recovered from the disease at the time of sample collection (7±50 days after the outbreak), and the adult animals did not show any clinical signs during the outbreak. In agreement with this observation no RV shedding was detected in any of the convalescent animals or in the healthy young guanacos (none of 51). However, the serum of all sampled animals, except for the sick new-borns, was positive for RV antibodies, including the adult guanacos (45 of 45) ( Table 1 ). The high antibody prevalence detected in both farms (95%) adds evidence indicating that guanacos, as observed in previous studies of other South American camelids, such as Lama pacos (Rivera et al., 1987) and Lama glama (Puntel et al. 1999 ) are susceptible to rotavirus infection. Further serologic analysis indicate that RV antibody titres in convalescent animals in farm A ranged from 64 to 4096 [geometric mean titre (GMT) 637], whereas in farm B, they ranged from 256 to 16 384 (GMT 1326). Young guanacos captured after the outbreak in farm A had an RV antibody GMT of 320 (64±1024), whereas the antibody titres of healthy adults in farm B ranged from 64 to 4096 (GMT 193) (Fig. 1) . These results showed a similar distribution of high antibody titres in the groups of convalescent and healthy guanacos. As it was not possible to obtain paired serum samples from the affected animals, corresponding to the acute and convalescent phase of the disease, serological con®rmation of the viral infection by seroconversion was not possible. However, the presence of very high antibody titres in some of the convalescent animals (1:16 384) suggests recent exposure to RV. In contrast, all animals tested were negative for BCV antigen in faeces (none of 53) and BCV antibodies in serum (none of 47), suggesting that this pathogen was not circulating in the guanaco population under study and was not involved in the diarrhoea outbreak. The two RV strains detected by ELISA were successfully propagated in roller tubes containing con¯uent monolayers of MA-104 cells in the presence of pancreatin (5 lg/ml) (Hoshino et al., 1983) . Viral cytopathic effects were detected 24 h post-inoculation and the culture supernatants were positive for RV by ELISA after the ®rst and second passages. In order to con®rm RV diagnosis in stool specimens and its presence in tissue culture supernatants, both materials were examined by immuno-electron-microscopy (IEM) using described methods (Saif et al., 1977) to evaluate virus morphology. Typical rotavirus particles in size and morphology (60±75 nm icosahedral particles) were observed as shown in Fig. 2a,b. In addition, to investigate the electropherotype of each virus strain, RV genomic ds-RNA was extracted from the original faecal samples and tissue culture, using conventional techniques (Sambrook et al., 1989) . Double-stranded RNA segments were analysed by electrophoresis in 7.5% polyacrylamide gels (PAGE) and visualized by silver staining (Laemmli, 1970) . The detected strains showed 11 dsRNA segments with a band distribution pattern (4:2:3:2) that is characteristic of group A RV. However, each strain had a distinctive long electropherotype (minor migration differences), suggesting the (Fig. 3) . In the present study, in addition to previous reports made in llamas and alpacas, the circulation of RV was con®rmed among another species of South American camelids, Lama guanicoe, not only by positive serology but also by the detection and isolation of RV from faecal samples from new-born guanacos with acute diarrhoea. No evidence for the circulation of other viral agents associated with neonatal diarrhoea, such as BCV was observed by ELISA or IEM. However the detection of E. coli in farm A and Salmonella sp. in farm B indicated that several enteric pathogens were involved in these two diarrhoea outbreaks. The absence of RV antibodies in the two sick animals could be related to a failure of passive transfer of maternal antibodies from the dam to the new-born. Previous studies in camelids have shown that intestinal absorption of immunoglobulins occurs during the ®rst 24 h after birth, being maximal between 8 and 12 h post-partum (Murphy, 1989; Adams and Garry, 1994) , and that failure of passive transfer is a major factor leading to mortality in neonatal camelids (Drew and Fowler, 1995) . The present data suggests an absence of adequate levels of passive protection that may be associated with the high mortality observed after the diarrhoea outbreak. The capture of animals at several months of age, instead of a few days of age was recommended to the farmers to avoid the possible interruption of proper colostral and lactogenic antibody intake. Rotavirus diagnosis was con®rmed by IEM and PAGE. As infections with group A RV have been detected in animal species in Argentina (Barrandeguy et al., 1988; Bellinzoni et al., 1989 Bellinzoni et al., , 1990 Mattion et al., 1989; Parren Äo et al., 1997; Costantini et al., 1999) , it would be interesting to investigate whether these rotavirus outbreaks in Lama guanicoe were due to an autochthonous guanaco RV strain or transmission from a domestic species. Characterization assays and sequence analysis of the isolated strains are in progress to investigate these possibilities. To the authors' knowledge, this is the ®rst report of detection and isolation of RV associated with neonatal diarrhoea in Lama guanicoe. 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Cornell Vet Molecular Cloning. A Laboratory Manual Evaluation of two antigen-capture ELISAs using polyclonal or monoclonal antibodies for the detection of bovine coronavirus The authors are indebted to Adriana McGuirre from Fundacio Ân Habitat, D. Garrido from Chacay' farm, Dr C. Cabral and personnel from`Direccio Ân de Fauna de la Secretarõ Âa de Recursos Renovables' for their valuable collaboration. This work was supported with Grants from UBACyT/ I2623/99-IVO1, Academia Nacional de Agronomia y Veterinaria, FONCyT 08/01740 and PICT-08/04687 from ScyT-Argentina and a grant from The Ohio Agricultural Research and Development Center (OARDC) for International Collaboration, The Ohio State University-USA. A.A.S. is member of the CONICET Research Career (CONICET-Argentina). V.P.'s work is supported by a CONICET internal post-degree scholarship and V.C. by a CIC PBA scholarship.