About the Author(s)


Anthony J. Mills Email symbol
Department of Soil Science, Faculty of AgriSciences, Stellenbosch University, Stellenbosch, South Africa

Tercia Strydom symbol
Scientific Services, South African National Parks, Skukuza, South Africa

Jessica L. Allen symbol
OneWorld Sustainable Investments, Cape Town, South Africa

Julia Baum symbol
African Wildlife Economy Institute, Stellenbosch University, Stellenbosch, South Africa

Citation


Mills, A.J., Strydom, T., Allen, J.L. & Baum, J., 2023, ‘Potential geochemical constraints on tree seedlings in northern Kruger National Park grasslands’, Koedoe 65(1), a1773. https://doi.org/10.4102/koedoe.v65i1.1773

Short Communication

Potential geochemical constraints on tree seedlings in northern Kruger National Park grasslands

Anthony J. Mills, Tercia Strydom, Jessica L. Allen, Julia Baum

Received: 19 Apr. 2023; Accepted: 27 June 2023; Published: 26 July 2023

Copyright: © 2023. The Author(s). Licensee: AOSIS.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

In the northern Kruger National Park (KNP), South Africa, there are extensive areas of treeless grassland interspersed within treed savannas (Figure 1) (Pienaar 1963). To the authors’ knowledge, there have been no investigations into the factors preventing trees from establishing in these grasslands.

FIGURE 1: Study site location and habitats within the Kruger National Park (KNP), South Africa, showing (a) the distribution of treeless grasslands in the northern Kruger National Park, photographs of (b) grassland and (c) adjacent treed savanna and (d) the location of sampling plots (~100 m2) in grassland (squares) and savanna (triangles).

There are many potential factors that could lead to poor recruitment of tree seedlings in these grasslands relative to the savanna matrix. Such factors, including for example competition from grass and herbivory pressure (Morrison et al. 2019; Porensky & Veblen 2012; Riginos 2009; Van Auken & Bush 1988), warrant further investigation. Soil nutrients are likely to interact with herbivory and grass competition in the tree constraint given that sub-optimal availability of any particular nutrient will adversely affect a recently germinated tree seedling’s rate of growth as well as recovery from herbivory (Cohn, Van Auken & Bush 1989; Kambatuku, Cramer & Ward 2011; Vadigi & Ward 2012). Consequently, the soil surface of the grasslands and adjacent savannas was sampled as a first step in assessing the potential influence of nutrition on grass competition as well as tree seedling growth immediately after germination.

Forty composite soil samples (0 cm–2 cm) were taken from an extensive treeless grassland and adjacent savanna north of Letaba Rest Camp in the KNP, South Africa with permission from the South African National Parks. The dominant tree in the savanna is Colophospermum mopane. The underlying geology of both vegetation types is Letaba basalt. The study did not include excavation of soil pits and consequently the researchers did not classify the soils. However, a study by Venter (1990) near the study site found that the two most common soil forms were Oakleaf and Valsrivier. The Oakleaf soil form is a cumulic soil characterised by young, unconsolidated sediment, either colluvial, alluvial or aeolian in origin (Fey 2010) with A horizons ~10 cm–20 cm deep (SCWG 2018). The Valsrivier soil form is a clay-enriched duplex soil (Fey 2010) with A horizons ~15 cm–25 cm deep (SCWG 2018). Each composite sample comprised 8–10 sub-samples taken from 100 cm2 within a plot of 100 m2. The samples in savannas were taken outside of tree canopies. The influence of land use on the geochemistry of the 0 cm–2 cm surface layer of the soils sampled is likely to be insignificant because no agriculture has taken place in the area for at least one century.

Soil samples were air-dried and sieved to < 2 mm. pH was determined using a 1:2.5 soil:1M KCl solution. Inductively coupled plasma mass spectrometry was used to analyse: (1) potassium; calcium; magnesium and sodium (1% citric acid extract) (Division of Chemical Services 1956); (2) phosphorous (1% citric acid extract) (Du Plessis & Burger 1965); (3) sulfur (calcium phosphate extract) (Beaton, Burns & Platou 1968); (4) boron (hot water extract) (Bingham 1982); and (5) manganese, copper, zinc (0.02M di-ammonium ethylenediaminetetraacetic acid extract) (Beyers & Coetzer 1971; Trierweiler & Lindsay 1969). Organic carbon was determined using the Walkley-Black method (Nelson & Sommers 1982; Walkley 1935).

The results showed that relative to the savanna the soil surface in the grassland was enriched in phosphorous, magnesium and zinc, impoverished in boron and more alkaline (Figure 2). The only nutrient that emerged from this study as potentially constraining tree seedlings shortly after germination in the grassland was boron, with a mean concentration of 0.4 mg kg−1. Assessing whether a concentration of 0.4 mg B kg−1, extracted using hot water from a specific soil, will impede growth in a particular plant species is complex, as multiple soil properties such as pH, clay mineralogy, soil water content and phosphate concentration influence boron uptake by roots (Bell 1997; Shorrocks 1997). Notwithstanding this complexity, on finding such concentrations of B and assuming that the goal (at any given site, regardless of soil properties, climate, or plant species involved) was to optimise plant growth, it would be prudent, based on the existing literature (see e.g., Bell 1997; Tariq & Mott 2007), to experimentally determine whether boron is limiting plant growth. Particularly relevant studies in this context include those by Li et al. (1978), Rashid, Rafique and Bughio (1994), Rafique et al. (2002) and Yadav et al. (2017), which found that concentrations of 0.4, 0.5, 0.6 and 0.5 mg B kg−1, respectively, restricted the growth of the plants under investigation.

FIGURE 2: The soil chemistry of composite pedoderm samples from grassland (n = 20) and adjacent treed savanna (n = 20) at the study site in the northern Kruger National Park, South Africa.

It is noteworthy that boron requirements of dicotyledonous plants, such as the tree seedlings at the study site, are likely to be considerably greater than those of the grass species (Bell 1997; Tariq & Mott 2007). Scarcity of boron would consequently be expected to constrain the tree seedlings more than the competing grasses. It is also noteworthy that within fertilisation experiments in the Brazilian Cerrado, plant growth was constrained by deficiencies of boron more than deficiencies of any other nutrient (Lannes et al. 2020), and that a pot experiment in South Africa, using a wide range of soils from natural ecosystems, found that boron was the nutrient most strongly correlated with growth of indigenous tree seedlings (Wakeling, Cramer & Bond 2010). Pot experiments with a wide range of local grass and tree species are likely to be of value for further investigation into the hypothesis that a relative scarcity of boron in the northern KNP grasslands enhances the competitive strength of grasses more than recently germinated tree seedlings. Given that nutrient deficiencies in plants are usually identified most effectively using foliar nutrient analyses, the authors suggest that such pot experiments should include nutrient analyses of foliage as well as soil.

Acknowledgements

The authors would like to thank SANParks for permission to take soil samples in the Kruger National Park, the Western Cape Department of Agriculture laboratory at Elsenburg for soil chemical analysis, Antoni Milewski for discussions on concepts presented, section ranger Karien Keet and game guard Desmond Siza for support in the field, Adelé Cormac, Selina Mochrie, Zurelda M. le Roux and Ruan de Wet for technical support, and Nicholas Salonen and Ruan van Mazijk for preparation of the manuscript for submission.

Competing interests

The authors declare that they have no financial or personal relationships that may have inappropriately influenced them in writing this article.

Authors’ contributions

A.J.M. conceived and carried out the study and acquired funding. J.B. and T.S. provided administrative assistance and T.S. facilitated access to the field site where J.B. and A.J.M. conducted field work. J.B. and J.L.A. conducted a literature review for this study. With A.J.M., J.L.A. conducted the statistical analyses. J.L.A. prepared the figures. A.J.M. wrote the manuscript, with all authors approving its contents.

Ethical considerations

Ethical clearance to conduct this study in the Kruger National Park was obtained from South African National Parks (permit reference number MILA1364).

Funding information

The authors would like to gratefully acknowledge the South African Department of Environmental Affairs, Natural Resources Management Programme and the National Research Foundation (grant number FA2005040700027) for funding this research.

Data availability

The raw data from this study are available at the Stellenbosch University Institutional Research Data Repository ‘SUNScholarData’: https://doi.org/10.25413/sun.23659920.

Disclaimer

The views and opinions expressed in this article are those of the authors and do not necessarily reflect the official policy or position of any affiliated agency of the authors.

References

Beaton, J.D., Burns, G.R. & Platou, J., 1968, Determination of Sulphur in soils and plant material, Sulphur Institute, Washington, DC.

Bell, RW., 1997, ‘Diagnosis and prediction of Boron deficiency for plant production’, Plant and Soil 193(1), 149–168. https://doi.org/10.1023/A:1004268110139

Beyers, C. P. De L. & Coetzer, F.J., 1971, ‘Effect of concentration, pH and time on the properties of Di-Ammonium EDTA as a multiple soil extractant’, Agrochemophysica 3(4), 49–53.

Bingham, F.T., 1982, ‘Boron’, in AL (ed.), Methods of soil analysis: Part 2 chemical and microbiological properties, pp. 431–47, 9, American Society of Agronomy, Soil Science Society of America, Madison, WI.

Cohn, E.J., Van Auken, O.W. & Bush, J.K., 1989, ‘Competitive interactions between Cynodon Dactylon and Acacia Smallii seedlings at different nutrient levels’, American Midland Naturalist 121(2), 265–272. https://doi.org/10.2307/2426030

Division of Chemical Services, 1956, Analytical methods, Pretoria, South Africa.

Du Plessis, R.F. & Burger, R.D.T., 1965, ‘A comparison of chemical extraction methods for the evaluation of phosphate availability of top soils’, South African Journal of Agricultural Science 8(4), 1113–1122.

Fey, M.V., 2010, ‘Introduction to the soil groups and the naming of forms and families’, in M.V. Fey (ed.), Ch. 1 in Soils of South Africa, p. 11, Cambridge University Press, Cambridge.

Kambatuku, J.R., Cramer, M.D. & Ward, D., 2011, ‘Savanna tree–grass competition is modified by substrate type and herbivory’, Journal of Vegetation Science 22(2), 225–237. https://doi.org/10.1111/j.1654-1103.2010.01239.x

Lannes, L.S., Venterink, H.O., Leite, M.R., Silva, J.N. & Oberhofer, M., 2020, ‘Boron application increases growth of Brazilian Cerrado Grasses’, Ecology and Evolution 10(13), 6364–6372. https://doi.org/10.1002/ece3.6367

Li, B.H., Li, W.H., Kui, M.C., Chao, W.S., Jern, H.P., Li, C.R. et al., 1978, ‘Studies on the cause of sterility of wheat’, Journal of the Northeastern Agriculture College 3(1), 19.

Morrison, T.A., Holdo, R.M., Rugemalila, D.M., Nzunda, M. & Anderson, T.M., 2019, ‘Grass competition overwhelms effects of herbivores and precipitation on early tree establishment in Serengeti’, Journal of Ecology 107(1), 216–228. https://doi.org/10.1111/1365-2745.13010

Nelson, D.W. & Sommers, L.E., 1982, ‘Total carbon, organic carbon and organic matter’, in: A.L. Page, R.H. Miller & D.R. Keeney (eds.), Methods of soil analysis: Chemical and microbiological properties, pp. 539–579, American Society of Agronomy, Soil Science Society of America, Madison, WI.

Pienaar, U.D.V., 1963, ‘The large mammals of the Kruger National Park – Their distribution and present-day status’, Koedoe 6(1), 1–37. https://doi.org/10.4102/koedoe.v6i1.810

Porensky, L.M. & Veblen, K.E., 2012, ‘Grasses and browsers reinforce landscape heterogeneity by excluding trees from ecosystem hotspots’, Oecologia 168(3), 749–759. https://doi.org/10.1007/s00442-011-2123-9

Rafique, E., Rashid, A., Bhatti, A.U., Rasool, G. & Bughio, N., 2002, ‘Boron deficiency in cotton grown on Calcareous Soils of Pakistan I. Distribution of B availability and comparison of soil testing methods’, in H.E. Goldblach, P.H. Brown, B. Rerkasem, M. Thellier, M.A. Wimmer & R.W. Bell (eds.), Boron in plant and animal nutrition, pp. 349–358, Kluwer Academic/Plenum Publishers, New York, NY.

Rashid, A., Rafique, E. & Bughio, N., 1994, ‘Diagnosing boron deficiency in rapeseed and mustard by plant analysis and soil testing’, Communications in Soil Science and Plant Analysis 25(17&18), 2883–2897. https://doi.org/10.1080/00103629409369232

Riginos, C., 2009, ‘Grass competition suppresses Savanna tree growth across multiple demographic stages’, Ecology 90(2), 335–340. https://doi.org/10.1890/08-0462.1

Shorrocks, V.M., 1997, ‘The occurrence and correction of Boron deficiency’, Plant and Soil 193(1), 121–148. https://doi.org/10.1023/A:1004216126069

Soil Classification Working Group (SCWG), 2018, Soil Classification: A Natural and Anthropogenic System for South Africa, ARC-Institute for Soil, Climate and Water, Pretoria.

Tariq, M. & Mott, C.J.B., 2007, ‘The significance of Boron in plant nutrition and environment – A review’, Journal of Agronomy 6(1), 1–10. https://doi.org/10.3923/ja.2007.1.10

Trierweiler, J.F. & Lindsay, W.L., 1969, ‘EDTA-Ammonium Carbonate Soil Test for Zinc’, Soil Science Society of America Journal 33(1), 49–54. https://doi.org/10.2136/sssaj1969.03615995003300010017x

Vadigi, S. & Ward, D., 2012, ‘Fire and nutrient gradient effects on the sapling ecology of four Acacia species in the presence of grass competition’, Plant Ecology 213(11), 1793–1802. https://doi.org/10.1007/s11258-012-0134-1

Van Auken, O.W. & Bush, J.K., 1988, ‘Competition between Schizachyrium Scoparium and Prosopis Glandulosa’, American Journal of Botany 75(6 Part 1), 782–789. https://doi.org/10.1002/j.1537-2197.1988.tb13501.x

Venter, F.J., 1990, ‘Letaba land system’, in A classification of land for management planning in the Kruger National Park, pp. 277–279. PhD thesis. University of South Africa.

Wakeling, J.L., Cramer, M.D. & Bond, W.J., 2010, ‘Is the lack of leguminous savanna trees in grasslands of South Africa related to nutritional constraints?’, Plant and Soil 336, 173–182. https://doi.org/10.1007/s11104-010-0457-4

Walkley, A., 1935, ‘An examination of methods for determining organic carbon and nitrogen in soils1. (with One Text-Figure.)’, The Journal of Agricultural Science 25(4), 598–609. https://doi.org/10.1017/S0021859600019687

Yadav, S.N., Singh, S.K., Kumar, O., Latare, A.M. & Kumar, M., 2017, ‘Evaluation of a suitable extractant and determination of critical limit of Boron in soil and mustard (Brassica Juncea L.) plant by various extractants in inceptisols of Varanasi’, Communications in Soil Science and Plant Analysis 48(10), 1145–1153. https://doi.org/10.1080/00103624.2017.1323103