; 587 J7 :opy 1 fexmiMENTS AND INVESTTOATIONS Office of Experiment Stations, CON DUCT KT) AT THE Ar;i I u PENNS YLV ANI A STATE COLLEGE, 1881-2, By Prof. W. H. JORDAN, Pennsylvania State College. HAKRI8BURG, PA.: LANE S. HART, PRINTER AND BINDER. 1882. EXPERIMENTS AND INVESTIGATIONS CONDUCTED AT THE PENNSYLVANIA STATE COLLEGE, 1881-2. By Prof. W. H. JORDAN, Pennsylvania Slate College. HARRISBURG, PA. : LANE S. HART, PRINTER AND BINDER. 1882. <^ (o^,^\ ■VIAft 11 1905 0. of 0. EXPERIMENTS AND INVESTIGATIONS. Effecp of Period of Cutting and of the Soil upon the Composition OF Timothy Hay. It is generally understood that hay varies in composition, and conse- quently in value, according to the conditions under which it is grown, and the time at which it is cut. Yery much has been learned concerning the nature and causes of such variations, and of their effect upon the nutritive value of hay ; but v;^e are still far from possessing the necessary amount of information on some points. To aid in securing the desired knowledge was the aim of this investigation. What is given in the following pages is simply a report of progress, as other samples of hay are to be analyzed. Of course all such work has for its object a better understanding of how to secure the maximum nutritive value in the grass or hay used for feeding, and in order that the utility of the analyses herewith reported, and of those to follow, may be seen, there is given a brief statement of some of the main facts concerning the ingredients of cattle foods, and their relation to animal nutrition, followed by a resume of present knowledge pertaining to the main points under consideration. Tlie Ingredients of Plants and tlielr Office In Animal Nutrition. All vegetable cattle foods are made up of four classes of substances, viz : Nitrogenous organic substances^ (included, heretofore, under the general names of albuminoids or protein,) carbo-hydroles, fats oro^7s,and mineral substa7ices, and the value of any given food stuff depends upon the relative percentage which it contains of these different ingredients. An animal grows, exercises muscular force, produces milk and youns,", and keeps up a supply of bodily heat, and these different food elements furnish the means whereby this is done. Moreover, each of these different classes of sub- stances has its own peculiar part to play in maintaining animal life. One class may be able to do what another cannot. Protein or Albuminoids. — These terms, sometimes one and sometimes the other, have been used to designate the organic nitrogenous constituents of plants as a class. It has been assumed, until lately, that essentially all of the nitrogen of plants is combined to form true albuminoids, compounds of which muscular tissue, (lean meat,) the white of an egg, the flesh of * For the plans of the experiments and investigations herewith reported, and for the accompanying analyses, I am responsible. For the c ireful and patient supervision necessary to tiie successful prosecution of experimental work, credit belonijs to the superintendents of the Central and Eastern Experimental Farms, Mr. W. C. Patter- son and Mr. J. F. Hickman. The work was undertaken and will be continued in the hofie that some conclusions will be reached that will prove to be oi advantage to the farmer as a producer. The results here reported may serve possibly to give some idea of what might be accomplished by a well equipped experiment station, where land, means, and men should be centralized in one efficient organizition. It is but fair to say that the analytical work has been possible through the great pro- gress that has been made in tlie development at the College of a working chemical laboratory within the past few years, so that analyses can now be executed with toler- able rapidity and according to modern methods. W. H. Jordan. fishes, and the gluten of wheat are good examples. It is now known that a portion of the nitrogen may be combined to form compounds not al- buminoid in their character, and to this latter class has been given the term amides. As epecial interest attaches to the occurrence and properties of these compounds, they will receive fuller mention later. The albuminoids proper are considered to be the mostim[)ortaut ingredients of cattle foods. From them alone can be i)rodnced the muscular tissue of the animal body, as well as the casein and albumen of milk. Butter fat is, undoubtedly, formed from them, and a portion cf the body fat, and muscular force is somehow dependent upon tlieir presence in the food. While the most important nutritive ot]lces of albuminoids are indicated al)Ove, they probably, at all times, directly aid in keeping the animal warm, and in case the ration is composed entirely of albuminoids, can be made to furnish all the fuel for the mamtenance of bodily heat. In short, there is nothing these compounds cannot do in sustaining life, unless it be the supplying of mineral substances. A fter having gone through the digestive processes, the products of their decomposition make up quite a portion of the fertilizing value of farm manures. Carbo-hydrates includes such compounds as crude fiber (cellulose and lignose,) starch, sugar, gums, &c. j^hese bodies contain no nitrogen, and, therefore, cannot serve as the source of llesh or the principal compounds of milk. Just what their relation is to muscular force is not yet fully de- tei'Vnined. Their chief office seems to be to supply fuel for keeping up animal heat, though they undoubtedly' aid in the formation of fat in the herbivora, but not in the carnivora. The fats serve the purpose of storing animal fiit, and are also burned to kee]) the animals warm, one pound of fat being worth, for fat and heat-form- ing purposes, probably not far from two pounds of starch or sugar. The percentages of tat, as given in fodder tables, are too large, owing to the fact that the ether used in extracting them dissolves out other sub- stances. Both the fats and carbo-hydrates have an indirect value in that they serve to protect the albuminoids from destruction, and thus make greater flesh or milk production possible. luflneucc of Fertility. Fodder manufactured from the same species of plant does not generally have the same comi)osition in two cases where there has been a dilf.rence in the conditicms of growth and treatment. Past investigations seem to indicate that the state of fertility of the soil has a prominent influence in determining not onl}^ the quantity, but the quality of farm crops. Shhes- ing* found that the ash of tobacco varied greatly in composition, according to the fertilizer applied. It is a well-known fact that the percentage of sugar in sugar beets can be diminished or increased according to the method of manuring. Ritthausen and Pott, f Krenslcr and Kern,| and especially Lawes and Gilbert, have found that the application of an abundance of nitrogenous manures to wheat, causes an increased percentage of nitrogen in the grain. EmmerlingS found that hay grown upon low land of good quality con- tained nearly two per cent, more of protein than hay grown upon poor land of the same general character. Dr. Armsby T[ cites analyses of two samples of ha3% one being taken " from * .TaVireshericht der Agr., Chem. IIT., p. 81. t Ibid. XVI. I. p. 304. t Iljid. XVIII, I, p. 253. § Ibid., p. 2(59. 1 Manual of Cattle Feeding, pp. 289-290. a part of the field which was. in an ordinary state of fertility," and the other " from spots where the exci'ement and urine of the grazino- animals had caused an especial luxuriant growth." The former contained only eleven per cent, of protein, the latter over twenty per cent. There is found to be quite a difference between American and German hays, the latter being the better. Below is a comparison of the average composition of nine (9) samples of American timothy with the composition of German timothy : American timothy, German timothj^, . Water. Per cent. 18.50 14.3 Protein. Per cent. 6.16 9.7 Fiber. Per cent. ^8.91 22.7 Nitrogen, free ex- tractive matters. Per cent. 45.85 45.8 Fat. Percent. 1.68 3.0 It is not improbable that the difference seen above is due to the more thorough cultivation practiced in Germany, although a partial cause may be found in climatic conditions. Iiifliieuce of tlic Stage of Growtli. Hay made from early cut grass differs from that made from late cut, in the following particulars : 1. It contains a larger percentage of nitrogen. Whether this is due to the presence of a greater percentage of albuminoids or not will be discussed later. 2. It contains a smaller percentage of crude fiber. 3. It contains larger percentages of fat and of ash. 4. One effect of the above differences in the composition of early and late cut hay, is to render the former more digeslible, which is certainly in favor of the early cutting of hay. AVhether tliere are any compensating advan- tages in late cutting remains to be seen. The question of the relative values of earl^^ and late cut hay is. at pres- ent, much discussed. The opinion has gained ground somewhat of late that the value of early cut hay has been over-estimated. This opinion has doubtless been strengthened by the claim that in the true grasses quite a percentage of the nitrogen in the young plant is not in the albuminoid form, and that the relative percentage of albuminoid nitrogen increases with age. The Occurrence of Amides in Grass, and their Influence upon Nutritive Value. The method which chemists have been forced to take for the estimation Ol albuminoids, has been based upon an assumption, viz : That, essentiall3^ all the nitrogen of plants exists in the albuminoid form. Acting on this assumption, and knowing that the average percentage of nitrogen in the various albuminoid substances is about sixteen (16) per cent, of the whole substance, it has been customary to determine the amount of nitrogen and multiply this by 6^ in order to obtain the amount of albuminoids. Were there no nitrogenous substances in hay or other cattle foods, save albumi- noids, such a method of determination would probably give quite a close approximation to correct results. Later investigations show, however, that our common fodder plants contain a variety of nitrogenous compounds, some of which are not albuminoid, either in chemical form or in properties. This renders the analj'sis of cattle foods, and the discussion of their values, more complicated. Dr. H. P. Armsby,* in connection with an investigation upon the non- albuminoid nitrogen of hay and otiier food stuffs, has made an admirable review of the whole question, and there is here presented a brief resume of his very complete article, with an occasional comment. Kluds and Occurrence of IVou-albutnluold Nltrosrenoua Substances. 1. Nitrates, nitrites, and ammonia salts occur in plants, most largely in root crops. [These are in the form of mineral salts, which have no signifi- cance in connection with animal nutrition, and they have to be considered in the analysis of food stuffs.] 2. The only nitrogenous organic substances, not albuminoid, which oc- cur in sufficient abundance in cattle foods to demand attention, are the so- called amides, a. nameapjilied not only to amides proper, but to other bodies closely resembling them. These substances are really organic combinations of ammonia. [A mides cannot be considered so highly organized compounds as are the albuminoids.] Functions of Amides In the Plant. 1. It is pretty clearly shown that all transfer of albuminoids from one *)art of a plant to another is accomplished by their being transformed into amities, in which form the movement occurs, and from which albuminoids are rebuilt where new plant substance is forming. [Inasmuch as amides are soluble and easily diff'usible, and as albuminoids possess neitiier of these properties to an}' great extent, there is every reason why some such -trans- formations should occur. It is an undoubted fact that the albuminoids in the seeds of grain and hay are formed from similar substances already ex- isting in the plant, and it seems quite probable that nitrogenous substance travels from the stalk to the seeds in the form of amides. | 2. A^nides have, in certain cases, been found to constitute a reserve of nitrogenous plant food, as in the case of fodder beets which have been found to contain quite a large quantity of these compounds. In the second year's growth, these amides find their way into the stalks and leaves, and are there converted into albuminoids. Occurrence of Anildes. From what has been said of the functions of amides, we should expect to find them in greater abundance in young plants, which, according to the results of the investigations of Kellner, seems to be the case. The riper the plant, the larger the proportion of albuminoid nitrogen according to Kelhier. Dr. Armsby found amides in all of twenty-one samples of coarse fodder, varying from 8.93 per cent, to 39.60 per cent, of total notrogen. [So far as can be judged from the dates of cutting, the hays from the youngest grass, do not, in Dr. Armsby's analyses, show a very much larger percent- age of amides than does the later cut hay. Six samples cut before Julj'^ 1 gave an average of only two per cent, more of niti'ogen combined as amides than the average of twelve samples cut after that time u{) to as late as Au- gust 15. All the samples were cut in Connecticut and New Hampshire.] Malt sprouts, wheat and rye bran, lupines and beans, roots, and ])otatoes, have all been found to contain considerable non-albuminoid nitrogen. Only a small portion of the nitrogen of cereal grains is in the amide form. ♦Report of Conn. Expt. Station, 1879. Relation, of Amides to Aulmal Nutrition. It is of course important to know what is the office of amides in sustain- ing or building up the animal body. 1. Certain experiments ?eem to show that amides can cause an increase of flesh in the animal, but this fact cannot be fully affirmed. 2. It is more probable that amides act as a protection to prevent albu- minoids from oxidizing, thus allowing more of the latter to take part in flesh formation, and so, in an indirect way, are as valuable as albuminoids. The laws of nutrition and scientific feeding standards as experimentally determined, are in no way invalidated by the discover}^ of this new class of compounds in cattle food. Comiiositiou of Samples of Hay Gro-wn on the Central Kxperimeutal Farm, nuder Different Conditions of Fertility, and Cut at Different Periods of Gro'wtli. On the 10th of May there was applied to a few square feet of grass land a mixture of dissolved bone, muriate of potash, and sulphate of ammonia, a complete fertilizer, containing all the ingredients which any soil would be likely to need in order to grow a luxui'iant crop of grass. The grass growing on the spot fertilized was almost all timothy. The general condi- tion of the land was such as to produce about one ton and a half of timothy hay per acre, being the limestone clay so common in Centre county. The fertilizers being ap])lied liberally, ^though but once,) the grass made very luxuriant growth, certainly more than double that of the adjoining- grass where no fertilizer was applied. In rapidity of development there seemed to be very little difference, the period of bloom being reached at about the same time in the two cases. Samples of both the grass fertilized and that immediately adjoining which was not, were taken at three periods of growth, as follows,* (all pure timothy :) 1. June 6. Heads just appearing. 2. June 23. Just beginning to bloom. 3. July 5. Somewhat past full blossom. . The Samples were weighed green immediately on cutting, were quicklj^ and carefully dried, and stored in paper bags. In the following table are given the weights of the different samples when green, and of the dry hay as analyzed, with the percentage of water dried out : With Fertilizers. Without Fertilizers. Period of GUOWTII. *Weight of grass taken. Weight of air dry hay. Per cent, of water evap- orated. Weight of grass taken. Weight of air dry hay. Per cent, of water evap- orated. First Second, Third, grams. 598.4 241.5 151.8 grams. 136.5 79.5 63.3 77.2 67.1 58.3 grams. 723.4 295.1 132.7 grams. 1SS.8 98 7 57.2 73.9 66.6 56.9 * The weights of samples taken have no reference to the yields of grass in the several cases. The hay was much drier when analyzed than it would have been if kept under ordinary conditions, having been stored in a diy room for about three mo nths. Two facts only are to be noticed in connection with the ~above~tabTe7viz : (1.) The youngest samples of grass lost seventeen to * Owing to absence ou a vacation, a fourth samiDle was not taken when the gra.ss yvas nearly ripe. nineteen per cent, of water more than the oldest, and (2) the grass fertil- ized, and making the largest growth, lost the most water in every case. The relative yields of grass were not taken into account, as other investi- gations on that point will be reported later. The following table shows the composition of the several samples of hay, the first column giving the water content when analyzed, the remaining columns showing the composition of the water-free substance : fi WiTH Fertilizers. "Without FERTiLizERb 100 parts water-free sub- 100 parts water-free sub- Period of Growth. stance contained. stance combined. tab A ^ . O . ■^ U (A o a; Si o f.% o <^/r % ■ ? Laboratory Number. U X2 6 CJ C ss OJ S32 p ^ Srs 63 Oh O O fe 1,2, and 3. 64 64 67 48 4, 5, 7, and 9, 56 52 57 57 63 61 48 6, 8, and 10, 49 *It should be reuienibered that the term total noii-albuiiiinoids nitrogenous matter. "amides" is used here to represent the 11 Using these percentages, we have the following quantities of digestible nutrients in one hundred pounds of the hays analyzed : Labokatory Number. 0) 6 >> JS > 6 ."m X2 m 3 a a O P^ 5^ & 5, 2, 4, 6, 9, 10, 7, 8, 9.72 6.16 8.68 5.40 3.13 2.28 3.95 2.58 2.86 2.16 40.10 45.98 44.91 46.11 45.53 45.60 44.62 45.86 46.02 46.23 1.82 1.08 0.83 1.62 1.08 92 1.04 1.02 0.96 0.97 1: 4 1: 7 1:12 1: 9 1:15 1:21 1:11 1:18 1:16 1 :22 Extra, . . Very good, Average, . Good, Average, . Poor, . . . Good, . . Poor, . . . Average, . Poor, . . . Heading out. In early bloom. Past blossom. Heading out. In early bloom. Past blossom. In bloom. Nearly ripe. In bloom. Nearly ripe. The " nutritive ratio " is the relation of the quantity of digestible nitro- genous material to the quantity of digestible carbo-h^-drates, the latter in- cluding two and one half times the fats. The extent to which hay is nitrogenous, is not only an indication of its capacity for milk and meat production, but also gives some idea as to the proper kind and amounts of bye fodder that should be fed with it. There is here inserted, for purposes of comparison, a table giving the content of total and digestible ingredients of various grades of German hays : >> Digestible NUTRIENTS. •a .= ■O ^■3 s a o o a = n a s g 3 ■°sc _ o3 j= ^ < < pR o Pm <1 ©■=« ^ Meadow hay, poor, . . . Do. fair, . . . Do. medium, . Do. very good. Do. extra, . . % % % % '^/r, % ^n ^/^ on what ingredients of the fodder does this loss fall ? KnoAving the percentage composition of the water-free substance in the two cases, we can calculate the amount of each class of ingredients in 100 pounds of dry substance in the corn as taken from the field, and in 91.47 pounds, as 1 * Water and other volatile substances, t Fat, chlorophyl, noist- ened asbestos. This method seemed to secure convenience and accuracy. * See report of Connecticut Experiment Station, for 1879, p. 109. fSee Beiderman's Central-blatt ftir Agr. Cham. Jahr. 9, Heft XII, S. 875. Ibid, Jahr. 10, Heft II, S. 134. X For preparation of copper hydrate, see last reference. 20 Parallel determinations were made in all cases. Comparative results ob- tained with and without the use of copper hydrate are as follows : Water, Total nitrogen, Albuminoid nitrogen : Arm8by's method, Stutzer's method, Amide nitrogen : Armsby's method, Stutzer's method, Percent, total nitrogen inaraide form . Armsby's method, Stutzer's method, No. 7. .81 ; .S49 . 857 ' .608 i .632 < .605 j .65 ( 10.27 .838 .62. .628 .218 .210 25.94 25.06 No. 8. .673; .674 . 699 ' .518 I .502 I .493 ! .517 I 10.00 .682 .51 .505 .172 .177 25.19 25.98 1.145 I 1.187 I .83 i .874 < .858 i .858 1 .852 ,314 .308 26.93 26.46 .595 I .614 I .649 ( . 625 I 9.46 .823 .218 .186 26.51 22.66 The results given are all that were obtained and are not selected. There seems to be no doubt that with hays the use of a p/ecipitant for the albu- minoids is unnecessary. The average percentage of nitrogen in the amide form was found to be only about one per cent, less when copper hydrate was used than when it was not, and as .01 per cent, error in the determina- tion of the nitrogen would be enough to account for this difference, we may regard it as insignificant. As to the absolute accuracy of either method we cannot speak with cer- tainty. It seems more probable that the albuminoid nitrogen is less than what these analyses show than that it is greater, for the reason that it seems more likely that non-albuminoid nitrogenous compounds would re- main in the extracted hay than that any albuminoids should remain in so- lution. Feeding Experiments. Feeding trials have been made with the eai'l^^ and late cut hay referred to in previous pages, also with cotton seed and corn meal. At both the Eastern and Central Farms, the animals fed have been fattening steers. Tlie trials were conducted with the utmost care, observing the methods and precautions indicated below. At each farm for each trial, four steers were selected, two being fed after one method, aud the other two after the method with waich it was desired to make comparison. The steers were selected so that in each lot of four, one pair should be as nearly like the other pair in size, weight, form, gen- eral ai)pearance and habit, as it was possible. The selection at the Central Farm of eight steers, was out of a lot of twenty-five. The rations that it was desired to test were weighed to the animals each day, any material they did not eat being also weighed. The weight of the steers was in no case recorded until the animals had been eating their rations for one week, fhe weighings were made weekly, at the" same hour in the day, alwa3^s before taking water. In all things ex- cept in wiiat they ate, the steers were treated as nearly alike as possible. Experiments In Feedluf? Early aud Late Cut Hay. As before stated, one lot (»f hay was cut while in bloom, and the other lot when approaching ripeness. At the Eastern Farm the hay was chopped 21 before feeding for convenience in weighing, and was fed with a small quan- tity of corn meal. After feeding two steers on early cut hay and two on late cut for a period of four weeks, the rations were changed about so that those getting early cut haj^ during the first period of four weeks, got late cut hay for an equally long period, and those getting late cut hay at first, got early cut for the last period. Below are the results : Tables sliowlug results of Experiment at the Eastern Farm. First Period, {S8 days.) Steers No. 1 and 2,* Steers No. 3 and 4, * early cut hay. late cut hay. Date of bep;inning, Dec. 22. Dec. 22. Date of ending-, Jan. 19. Jan. 19. Weight of steers December 22, 2,142 lbs. 2,150 lbs. Weight of steers January 19, 2,208 lbs. 2,158 lbs. Total gain in weight, 66 lbs. 8 lbs. Total quantity of hay eaten, 883.5 lbs. 816 lbs. Hay eaten per day, 31 .7 lbs. 29.1 lbs. Total quantity of corn meal eaten, 336 lbs. 336 lbs. Corn meal eaten per day, 12 lbs. 12 lbs. Second Period^ {28 days.) steers No. 1 and 2, Steers No. 3 and 4, late cut hay. early cut hay. Date of beginning, Jan. 26. Jan. 26. Date of ending, . Fel). 23. Feb. 23. Weight of steers January 26, 2,230 lbs. 2,180 lbs. Weight of steers February 23, 2,356 lbs. 2,290 lbs. Total gain in weight, 126 lbs. 110 lbs. Total quantity of hay eaten, 818 lbs. 812.5 lbs. Hay eaten per day, 29.2 lbs. 29 lbs. Total quantity of corn meal eaten, 336 lbs. 336 lbs. Corn meal eaten per day, 12 lbs. 12 lbs. The following is a summary of the two periods : Early cut hay. Late cut hay. Total quantity of hay eaten, . . . .' 1,696 lbs. 1,634 lbs. Hay eaten per day, (during 56 days,) 30.3 lbs. 29.2 lbs. Total quantity corn meal eaten, 672 lbs. 672 lbs. Corn meal eaten per day, 12 lbs. 12 lbs. Total gain in weight, 176 lbs. 134 lbs. Gain of two steers per day, 3.14 lbs. 2.4 lbs. Gain per pound of hay fed 104 lbs. .082 lbs. Relative value of each kind of hay as per experiment, is seen to be : Earlv cut : late cut :: 100 : 79. At the Central Farm, the steers were not fed at two periods, but the lots of two steers each, were fed for a while on the same ration, in order to ascertain the relative gain under similar conditions, so as to determine whether any difference in gain of weight when fed on" different rations would be due to differences in the animals. The hay was not chopped, and a smaller quantity of meal was fed than at the Eastern Farm. * The quantities given in the tables refer to the amounts fed to two steers. 22 Tal)le Sho^vlug Result of fixperlineiit at the Central Farm. Steers No. 1 and 2, Steers No. 2 and 3, early cut hay. late cut hay. Date of befiinning, Dec. 1. Dec. 1. Date of ending Feb. 25. Feb. 25. Weight of steers, December 1 n, 750 lbs. *1, 630 lbs. Weight of steers, February 25, i, 922 lbs. 1,702 lbs. Total gain in weight, 172 lbs. 72 lbs. Total quantitj' of hay eaten, 2,924 lbs. 2,234 lbs. Hay eaten per daj', (for 86 days,) 34 lbs. 26 lbs. Total quantity of corn meal eaten, 602 lbs. 602 lbs. Corn meal eaten per daj'^, 7 lbs. 7 lbs. Gain per pound of hay fed, 059 lbs. .032 lbs. Gain of two steers per dav, 2 lbs. .84 lbs. Relative value of each kind of hay as per experiment : Early cut : late cut :: 100 : 55. The method of comparison here adopted is for the last experiment hardly fair, because of the fact of a greater quantity of early cut hay being fed than of late cut. It would be fairer perhaps to determine whether the ex- cess of early cut hay fed is sufficient to account for the difference in gain, or whether something must be allowed for a difference in the quality of the two kinds of hay. The steers receiving early cut hay ate 690 pounds of hay more than did the others, and gained 100 pounds more, a gain of one pound for every 6.9 pounds of hay consumed. In other words, three pounds of haj'^ added to the daily ration of a single steer caused him to gain nearly one half pound per day more than he otherwise would have done. It is possible that we need seek for no other explanation for the better gain* of steers Nos. I and 2. It is but fair to say, however, that more early cut hay was consumed than of late cut, because of the greater palatableness of the former. In both cases all the hay was consumed that the steers would take. The steers have been sokl for six and a half cents per pound, giving a value of $6 50 to the 100 pounds excess of gain. This would make the excess of hay fed worth $18 80 per ton. Certainly the profits are greater from the early cut hay compared pound for pound with the late cut, and for thic- the greater palatableness of the former may fairly receive credit. As to the question of a difference in the capacities for growth of the two lots of animals, they Avere fed alike for a period of five weeks subsequent to the feeding on the two kinds of hay, and steers 1 and 2 gained 143 pounds, and steers 3 and 4 gained 173 pounds, showing that if either lot possessed a superior capacity for growth the advantage was with those fed on late cut hay. Let it be remembered in regard to all these results that they are the work of but a single year, and have value accordingly. So far, however, as any value attaches to the outcome of the experiments, the earlj' cut hay has the advantage. Experiments In Feeding Corn Meal and Cotton Seed Meal. The farmers of Pennsylvania fatten annually a large number of cattle. The principal food made use of for this purpose, beside coarse fodder, is cornmeal. Some farmers feed the cornmeal nearly pure, others mix with it considerable oatmeal, wheat bran to a limited extent, cotton seed and linseed meal. Opinions differ as to what food or mixture of foods is wisest. So far we have ver}^ little but opinion, if we except the experimental work of the Germans. Leaving the presentation of the scientific side of the question until later, it can be said that one practical inquiry is of great importance, viz : Can * The figures given refer to food and gain of two Bteers. 23 farmers profitably purchase the highly nitrogenous cattle foods that are for sale in our markets in order to combine them with the corn and coarse fodder produced on the farm ? Theories based on scientific investigation would answer the inquiry in the affirmative, so far as it is a question of proper combination of food ingredients, and so of an economical use of the ma- terial consumed. Of course the variable relative prices of these various food stuffs is something of which science can take no account, and the farmer must decide, from year to year, what he can or cannot afford to purchase. The great underlying principles in all practice in cattle feeding are those that determine the proper amounts and relation of nutrients in the ration, and these principles once understood it only remains for the farmer to purchase or produce these nutrients in the cheapest possible form. As in the case of early and late-cut hay, experiments have been con- ducted at both the Eastern and Central Experimental Farms. The num- ber of steers fed, and the precautions in feeding and weighing were the same as in tfie experiments on hay. A ration of corn meal and cornfodder has in each case been compared with one composed of corn meal, cotton seed meal, and cornfodder. In the latter ration the corn meal and cotton seed meal were mixed in the proportion of one hundred pounds of the former to forty pounds of the latter. The results of the experiment at the Eastern Farm are as follows : Tables Showing Results of Experiment at the Eastern Farm on Feeding Fattening Steers. First Period^ (56 days.) o*«>^^= K.«xi c steers 7 and 8, steers & ana b, Mixture of corn meal Corn meal alone. ^^^ ^^^^^^ ^^^^^ Date of beginning, December 15 December 15 Date of ending, February 9 February 9 Weight of steers, December 15, 2,110 lbs.* 2,110 lbs,* Weight of steers, February 9, 2,220 lbs. 2,336 lbs. Total gain in weight, 110 lbs. 226 lbs. Total quantity of cornfodder eaten, . . 674 lbs. 883 lbs. Cornfodder eaten per day, 12.3 lbs. 15.1 lbs. Total quantity cornmeal eaten, 1,660 lbs. 1,078 lbs. Corn meal eaten per day, 80.2 lbs. 19.6 lbs. Total quantity cotton seed meal eaten, . 481 lbs. Cotton seed meal eaten per day 7.8 lbs. Second Period^ {^2 days.) steers 5 and 6. Steers 7 and 8. Mixture of corn meal Corn meal alone, and cotton seed. Date of beginning, Feb. 16. Feb. 16. Date of ending, March 30. March 30. Weight of steers February 16, 2,254 pounds. 2,368 pounds. Weight of steers March 30 2,404 pounds. 1,510 pounds. Total gain in weight 120 pounds. 152 pounds. Total quantity of cornfodder eaten, . . 419 pounds. 524 pounds. Cornfodder eaten per day, 10 pounds. 12.5 pounds. Total quantity of corn meal eaten, . . . 830 pounds. 1,245 pounds. Corn meal eaten per day, 19.8 pounds. 29.7 pounds. Total quantity cotton seed eaten, .... 333 pounds. Cotton seed eaten per day, 7.9 pounds. A summary of the two periods shows the results to be as follows : * All the weights given in these tables refer to two steers. 24 Corn meal alone. Mixture of corn meal and cotton seed. Total quantity cornfodder eaten, .... 1,198 pounds. 1,252 pounds. Cornfodder eaten per daj^, (97 days,) . . 12.3 pounds. 13 pounds. Total quantity corn meal eaten, 2,905 pounds. 1,908 pounds. Corn meal eaten per day, 30 pounds. 19.7 pounds. Total quantity cotton seed eaten, 764 pounds. Cotton seed eaten per day, 7.9 pounds. Total gain in weight, 262 pounds. 376 pounds. Cost of food,* $46 57 |47 04 Cost of food per pound of increase, . . . 17.7 cents. 12.5 cents. The superintendent of the Eastern Farm states in his report that steers five and six were inferior in growing capacity to steers seven and eight. During the " first period," when the former lot was fed on the corn meal ration, their gain was very unsatisfactory, and much inferior to the gain of steers seven and eight that ate the mixture containing cotton seed. When, however, the rations were changed about so that the poorer steers received the cotton seed and corn meal, their increase in weight was equal to that of the better lot of steers that was given pure corn meal. The next table shows the results of the experiment at the Central Farm. Table Sbo^vliig Results of Experiment at Central Farm In Feeding^ Fattening Steers. Steers No. 5 and 6, Steers No. 7 and 8, corn meal alone, mixture of corn meal « and cottom seed. Date of beginning, Jan. 7. Jan. 7. Date of ending, . . ... April 1. April 1. Weight of steers, January 7, 1,835 lbs. 1,9.39 lbs. Weight of steers, April 1, 2,010 lbs. 2,200 lbs. Total gain in weight, 175 lbs. 261 lbs. Total quantity of cornfodder eaten, .... 840 lbs. 1,436 lbs. Cornfodder eaten per day, (84 da^'s,) .... 10 lbs. 17 lbs. Total quantity of corn meal eaten, 2.626 lbs. 1,344 lbs. Corn meal eaten per day, 31.3 lbs. 16 lbs. Total quantitj^ of cotton seed eaten, 672 lbs. Cotton seed eaten per day, 8 lbs. Cost of food, $43 37 ?38 15 Cost of ration per day, 52^ 45/5 In this case the rations were not changed about, so as to give the mix- ture of corn meal and cotton seed to Nos. 5 and 6. But in order to deter- mine the amount of error introduced by the different capacities for rrowth of the two lots of animals, the steers were fed alike for four weeks previous to beginning the experimental rations. Steers Xos. 5 and 6, gained 135 pounds during the four weeks, and steers Nos. 7 and 8, gained 194 pounds, or the two lots gained in the ratio of 100 to 144. While the experimental rations were fed, the gain of steers 5 and 6, was to the gain of steers 7 and 8, as 100 to 149. Or the relative gain of the two lots was the same when fed alike, and when fed the rations that were put to a comparative test. Now one lot ate about 32 pounds of corn meal per day, and the other lot only 16 pounds of corn meal, and 8 pounds of cotton seed ; that is, 1 pound of cotton seed when combined with the other foods was able to replace 2 pounds of corn meal. The superintendents of both farms report themselves as favorably im- pressed by the practical results of adding cotton seed meal to the ration of the steers that were being fattened. Looked at from the stand-point of profit, the outcome with the particular animals fed, and wuth relative prices as they are at present, was favorable to the use of the cotton seed. * In estimating the cost of the food, the fodder is valued at $5 per ton, the meal at one and one half cents per pound, and the cotton seed at $40 per ton. 25 The Scientific Side of Cattle Feeding. To show that with the particular circumstances and conditions involved in the experiments here reported, one method ^f feeding was productive of more satisfactory results than another, would amount to very little. Like circumstances and conditions may never occur again at the college farms or elsewhere. The relative supply of cattle foods and their relative prices change from year to year. To establish a fact with regard to the laws of animal nutrition, or some principle involved in all practice, would be to secure a lasting benefit. We need not so much to know that under certain conditions of practice certain results follow as to know the reasons why, or the principles involved. For instance, granting that a m xture of corn meal and cotton seed as fed in these experiments produced as much growth as a larger amount of pure corn meal, this may not be true simply because cotton seed and corn meal were fed together, but because the mixture furnished a more efficient combination of food ingredients than was the case with corn meal alone. It may be a question of the economical use by the animal organism of cer- tain quantities of protein and carbo-hydrates mixed in certain proportions rather than of cattle foods having certain names. If this be true, then we are not shut up to corn meal and cotton seed as the only means of securing the desirable combination, but can use an}' cattle foods that will furnish the ingredients we desire in the proper quantities and proportions. It must be remembered that protein, for instance, is a constituent of all cattle foods, and that it may be of more importance that we give an animal a certain quantity of it in a digestible form accompanied by proper amounts of other compounds, than that we supply it in any particular kind of food, whether it be hay, corn meal, wheat bran, or cotton seed. From the data given on previous pages, let us see what were the real differences between the rations fed in the experiments reported. Composition, of tlie Various Food Stuffs used. The composition of the hays fed can be seen on previous pages. The composition of the corn fodder, corn meal, and cotton seed, can be safely assumed from the average of a number of analyses of these food stuffs. No analyses were made of the particular samples used, because of entire lack of time. ^ o . a '^ O c5 A o C3 2^ X f 02 t-i '2 ja cS < P- o O P^ ^c •a Eastern Farm. , J Early cut hay, (No. 9,) • . . . 15.2 ibs. } Corn meal, 6 lbs. 13.3 5.34 18.64 .60 .50 6.78 3.9 .16 .25 Total, 1.10 10.68 .41 1:10.6 2 J Late cut hay, (No. 10,) . . . . 14.6 lbs. \ Corn meal, 6 lbs. 12.8 5.34 .38 .50 6.70 3.9 .15 .25 Total, 18.14 .88 10.60 .40 1:13.2 Central Farm. „ < Early cut hay, (No. 7,) . . . .17 lbs. \ Corn meal, 3.5 ibs. 14.9 3.11 18.01 .49 .29 7.82 2.27 .16 .15 Total, .78 10.09 .31 1:13.9 . ^ Late cut hay, (No. 8,) .... 13 lbs. I Corn meal, 3.5 lbs. 11.4 3.11 14.51 .28 .29 6.00 2.27 .13 .15 Total, .57 8.27 .28 1 : 15 8 28 Com Meal and Cotton Seed Meal. Kind and Quantity of Ration Fed a Single Animal. Eastern Farm. Cornfodder, 6.2 lbs., Corn meal, 15 lbs., Total, . . Cornfodder, 6.5 lbs , Corn meal, 9.9 lbs.. Cotton seed meal, 4 lbs.. Total, . Central Farm. Cornfodder, 5 lbs.. Corn meal, 15.7 lbs., fotal, Cornfodder, 8.5 lbs.. Corn meal, 8 lbs.. Cotton seed meal, 4 lbs.. Total, Digestible d e3 3 I a *S S 1 m St > K > s 5.27 .07 2.29 .02 13.34 1.25 9.75 .62 18.59 1.32 12.04 .64 1:10.3 5.52 .072 2.40 .02 8.80 .72 6.44 .41 3 60 1.46 .75 .58 18.01 2.25 9.59 1.01 1: 5.4 4.25 .06 1.85 .02 14.0 1.31 10.2 .64 18.25 1.37 12.05 .66 1:10. 7.03 .09 3.14 .03 7.11 .67 5.20 .33 3.69 1.46 0.75 .58 17.83 2.22 9.09 .94 1: 5.1 In another table let us place together the total quantities of digestible nutrients fed per day and per animal to each of the eight lots of steers, as shown in the two previous tables ; also the nutritive ratios and the gain per day, so that we ma^^ discover, if possible, what the relation is between gain and food. 1. Ration containing early cut hay, 2. Ration containing late cut hay, . 3. Ration containing early cut liay, 4. Ration containing lale cut hay, 5. Corn meal witliout cotton seed, 6. Corn meal with cotton seed, . 7. Corn meal without cotton seed, 8. Corn meal with cotton seed, . The German standard, per 1,000 pounds live weight, Digestible. ■ 6 Is S b > 3 2 di P-i Carbo- yd rates, lbs. H M ^ Lbs. Lbs. Lbs. Lbs. 18.64 1.10 10.68 .41 1:10.6 18.14 .88 10.60 .40 1:13.2 18.01 .77 10.09 .31 1:13.9 14.51 .57 8.27 .28 1:15.8 18.59 1.32 12.04 .64 1:10.3 18.01 2.25 9.59 1.01 1: 5.4 18.25 1.37 12.05 .66 1:10 17.83 2.22 9.09 .94 1: 5.1 26.0 2.5 15.0 0.50 1: 6.5 Lbs. 1.57 1.20 1.0 0.42 1.35 1.95 1.04 1.55 * These figures refer to the gain of single animals weighing throughout the experi- ments an average of about a thousand pounds, the first two lots weighing a little less, and the last two a little more. 29 One main and important difference to be noted in the above rations, is the relation between the quantity of digestible protein, and of the digesti- ble carbo-hydrates. It is seen that in rations six and eight, there is only the equivalent of a little over five pounds of digestible carbo-hydrates to each pound of digestible protein, while in rations two, three, and four, the ratio is very different, the digestible carbo-hydrates being present in nearly three times as large a relative quantity. Not only the relative but the ab- solute quantities of digestible nutrients differ very much in the various methods of feeding in the experiments, in one ration there being only about 0.6 pounds of digestible protein, and in others as much as 2.25 pounds. The variations in the amounts of digestible carbo-hydrates are very much less. It remains for future investigation to determine whether the increase in gain, that in these experiments has accompanied an increase of nitro- genous material in the food, is accidental or not. All present well-sub- stantied theories indicate that production, whether of meat, milk, or work, is largely dependent upon the so-called protein of the food, and that the relation in amount of this protein to the amount of other nutrients deter- mines largely the profits of feeding. The position taken is, that if too lit- tle nitrogenous material is contained in the combination of food stuflTs used, it would be necessar}^ to feed more than the animals could possibly consume in order to furnish suflicient protein to do the desired work, while if the ration be too highly nitrogenous, a waste of material occurs, and the ani- mal fails to use the nutrients given for the purposes of growth or produc- tion of milk. In the rations discussed here there is in no case, probabl}', an excess of protein, while in some cases it seems as if there was a defi- ciency. We believe that the question of the use of the nitrogenous waste pro- ducts, offered for sale in our markets, is one of great importance. It would be well for farmers to consider whether they cannot often realize a greater profit by purchasing these and selling the products of the farm, than by feeding the latter. I i J LIBRARY OF CONGRESS 002 672 039 4 ^