Bulletin No. 68. U. S. DEPARTMENT OF AGRICULTURE, •T* y^ OFFICE OF EXPERIMENT STATIONS. A. C. TRUE, Director. 557 B6 A DESGRIPTIOIN' SOME CHINESE yEfiETABLE FOOD AND THEIR NUTRITIVE AM) ECONOMIC VALUE Br WALTEE O. BLASDALE, Insiructor in Ghemistry, UniversHi/ of California* WASHINGTON: GOVERNMENT PRINTING OFFICE. 1899. Class Book T'/ssJ Bulletin No. 68. U. S. DEPARTMENT OF AGRICULTURE, OFFICE OF EXPERIMENT STATIONS. A. C. TRUE, Director. 4" V C> A DESCRIPTION OF AND THEIR NUTIUTIVE AND ECONOMIC YAIUE BY WALTER C. BLASDALE, Insfructor in Chemisfri/, Unirersity of California. WASHINGTON": GOVERNMENT PRINTING OFFICE. 1899. 1^ .ETTER OF TRANSMIITAL U. S. Department of Agriculture, Office of Experiment Stations, Washinrjton, i). C, July 15, 1899. Sir: I have the honor to transmit herewith a report by Walter C. Blasclale, instructor in chemistry at the University of California, de- scribing some Chinese vegetable food materials and their nutritive and economic value. These foods are used to a considerable extent by the Chinese popula- tion in San Francisco and other cities in the United States, and most, il not all, of them are staple articles of diet in China and the Orient. It seems probable that some of the vegetables may become generally and favorably known in the United States. Very little information has been hitherto available concerning many of these materials, and it is believed the report is a useful contribution to the knowledge of the food of mankind. Tlie report is respectfully submitted, with the recommendation that it be published as bulletin No. G8 of this Office. Respectfully, A. C. True, Director. Hon. James Wilson, Secretary of Ayriculture. 2 -0 , ^ CONTENTS Page. Introduction 5 Experimental methods 6 Roots and tubers 8 Sagittaria, or arrowhead 8 Taro 13 Water chestnut 15 Sacred lotus 17 Lily bulbs 19 Chinese sweet potatoes 22 Yam bean 23 Cassava, or manioc 25 Green vegetables and cucurbits 27 Green vegetables 27 Cucurbits 30 Seeds and grains 32 Soy beans 32 Phaseolus 36 Dolichos 37 Various water plants 39 Chinese millet 41 Fruits, nuts, and flowers 42 Fungi and alga^ 45 Miscellaneous substances 47 3 ILLUSTRATIONS. Page. Plate I. Plant from an imported tuber of Sagittaria sinensis 8 II. Drawiqgs of roots, seeds, etc 10 III. Drawings of starch grains 12 IV. Drawings of A'arious roots 14 V. Plant from a corm of J?/eoc7iari8 /«6c?'osa 16 VI. Upper portion of a plant of the black soy bean 32 VII. Mature plant of yellow soy bean " 34 VIII. I'lant of Phaseolus mumjo 36 4 SOME CHINESE VEGETABLE FOOD MATERIALS. INTRODUCTION. A visit to the Chinese quarter of San Francisco or any of the larger Pacific Coast cities will reveal to the eyes of a nonresident much that is both strange and interesting. Most of the curious roots, green vege- tables, seeds, and other articles of food making up the stock in trade of the Chinese merchant would be totally unknown and unsalable out- side of the narrow limits of the Chinese quarter. These articles are for the most part of Asiatic origin, many of them being directly imported from Canton, while others, though grown on American soil, are dis- tinctly Asiatic in character. Their presence here can be accounted for only on the supposition that they are of considerable importance in the domestic life of the Chinese. Evidently they are the equivalent of the materials that make up our own vegetable dietary and presumably possess ail intrinsic value for such a purpose. The thought then natu- rally arises, Might not some of these materials be turned to good account in the American household? Tradition assigns to the Chinese the highest attainment in the art of producing from a given area the greatest amount of ibod material. The latter result has been reached both by intensive cultivation and by the utilization of a great variety of food plants, so that all classes of soil and climate are made to yield their quota of food It would not be unreasonable to suppose, therefore, that the little-known regions of the Chinese Empire Irom which we have already obtained many useful plants might yield still others of real economic value. It can not be taken for granted, however, that all such materials, even though they do form an important part of the Chinese dietary, would be desirable introductions into our own. Of the numerous factors which must be considered in determining this question the composition of the vegeta- bles themselves is the most important. The amount of nutrients which they contain may be readily determined by submitting them to a chemical investigation. Such questions as digestibility, adaptation to American tastes, means of utilization, and cultural features of the plants themselves are all important elements in the i)roblem. Powerful incentives which might induce one to look for new varieties of food plants are not lacking. An increase in the number of vegeta- bles which are in cultivation might admit of the utilization of a greater diversity of soil and climate, or of a more profitable use of the regions 5 already in cultivation, or again might be desirable from a strictly dietetic standpoint. It has long been accei)ted as true that the Chinese are largely vege- tarian in their diet, and this apparently without serious detriment to their physical development. This statement, however, is not based on accurately compiled data, and, even if true, would be of no special sig- nificance until we know more about the composition of the Chinese vegetables. Apparently few analyses of Chinese food materials have been published, though Kellner, Kagai, Murai, and others have published a large number of analyses of Japanese food stuffs, many of which are produced by plants in common use among the Chinese. The incomplete character of some of these analyses and indeed of many others is likely to lead to erroneous conclusions. It is espe- cially desirable to discriminate between crude protein and digestible nitrogenous compounds when dealing with vegetable substances, since often as much as two thirds of the total nitrogen content consists of amido ' compounds or other substances of little or no nutritive value. EXPERIMENTAL METHODS. It was through the consideration of such facts as the above that the work des(;ribed in this artii^le was undertaken. It has been confined to a. study of the most important of the vegetable food materials found in the Chinese markets of San Francisco, though many substances of animal origin found there might profitably be submitted to similar investigation. The work naturally divides itself into two distinct lines of research: First, the botanical and horticultural features of the materials studied, and, second, the extent to which these materials ore used, the method of preparing and serving them, and the food value as shown by their chemical (•omi)osition. As a starting ])oiut for both lines of work it was necessary in each case to identify the plant from which the product under investigation was derived.' The identification proved in some instances a more diffi- cult matter than might have been anticipated, for, though many notes on tlif economic jdants of China are available, a large number of them contain couilicting statements and several difierent Chinese characters are often used to designate the same i)lant. In those instances in which the plants themselves could be grown by the author little difficulty was 'In conlirmation of this etatement see Bohraer. Landw. Vers. Stat., 28 (1883), p. 247. -An especial acivnowledfjiiient is duo Prof. John K. Fryer, of the department of oriental laugiiagos df the riiiversity of California, and to Mr. W. N. Fong, a student in the same institution, for assistance in this part of the work and for other courte- sies; also to Mr. Charles Ford, director of the botanic garden at Hong Kong, for identification of the roots of rittaria and Ijophotocaipus, 1894, pp. 9, 12. U. S. Dept. of Agr., Bui. 68, Office of Expt. Stations. Plate II. Drawings of Roots, Seeds, etc. 1, Seed aud young plant of the '-horn chestnut" n'mpa bispinosa); 2, Seed of Ginkgo biloba; 3, Tuber of Sagittnria sinensis; 4, Seed and young plant of Nelumbium speciosum; 5, Tuber of Sagittaria latifolia, sprouted; 0. Fruit and seed of the Chinese olive iCanarium album); r, Kernel of Canarium seed; 8, Root of Pueraria thunbergiana. 11 The starch grains of both species vary from orbicuhir to ovate in their jilane of greatest extent, are occasionally somewhat angled, and seldom exceed 30 // in diameter (PI. Ill, iig. 1). The hilum is somewhat eccentric and the striations are pronounced. With polarized light and a selenite plate they exhibit a slight play of colors. The composition of the two sorts of tubers is shown in Table 1. The only previous analysis of this vegetable which has been found is rei)orted by Kellner, and represents a Japanese sample of the tubers produced by S. sagittifolin . This is included in the table for purposes of com- parison. Table 1. — Composition of arrowhead tubers. a S o u '3 a 1 P4 J3 3? § a 1 1 S O .a i a a V ■B a Sagittaria latifolia : Original material Water- free substance Sagittaria sinensis: Original material Water free sii l)stance Sagittaria sagitlifoha : b Original material "Water- free substance p. ct. p. ct. 66. 88 4. 44 13.41 61.51 1 7.00 ! 18.18 P.ct. 3.98 12.02 4.71 12.24 5.76 17.38 P.ct. 0.46 1.39 2.29 5.94 1.29 3.88 P.ct. 0.76 2.29 .24 .62 .55 1.66 P.ct. 19.69 59.46 22.95 61). 89 P.ct. a 2. 49 7.51 a2.26 5.87 p.ct. 0.32 .93 P.ct. 0.98 2.97 .72 1.87 1.18 3.56 P.ct. 2.04 6.17 1.69 4.38 P.ct. 2.71 8.19 3.31 7.26 00.86 7.05 21.26 c22. 93 CC9.21 1.43 4.31 a Sample contained no reducing sugar.s. /Hieported by Kellner, quoted from Konig, Chemie di-r nieBscblichen Nahrungs- und Genus.smittel, 3. ed., 1, p. 705. c Carbohydrates by difference. A comparison of the dift'erent analyses shows that the figures obtained by Kellner differ little from the results obtained in this labo- ratory, and in no instance are the differences greater than might have been looked for in tubers grown in different regions. All three analy- ses show that these tubers contain considerably less water than the majority of our commonly cultivated root crops, and, like most such foods, only small amounts of fat, ash, and crude fiber. The most striking feature is the high content of crude protein, which, generally s[)eaking, is considerably h'gher than that of potatoes and similar vegetables. Kellner's analysis gives only total nitrogenous substance, but the other two show a remarkably high percentage of albuminoids, the difference in the crude protein of the two analyses being due almost entirely to nonalbuminoid nitrogenous matter^ so that, judging by these analyses, the two forms are about equally valu- able as far as albuminoid nitrogen is concerned. The superiority of the arrowhead over the potato in this respect is still more striking when it is remembered that about 50 per cent of the nitrogenous con- tent of the latter vegetable is of a nonalbuminoid character. Of the carbohydrate constituents, starch constitutes by far the most important part, though a considerable amount of some member of the cane-sugar group is present. In one analysis a small amount of some 12 a reducing sugar was found, but this may have heen due to a slight inversion of some body of the cane-sugar group. Furfurol-yielding compounds are also present, though only in small amounts. Some attempt was made to determine the exact nature of the nitroge- nous comi^ounds present. An aqueous extract of the dry residue of the second sample in the table yielded 15.G6 per cent of nitrogenous sub- stance; hydrochloric acid added to this extract precipitated 5.9G i)er cent of the original substance, 97 per cent being of a nitrogenous character. An extract of the freshly crushed tubers gave a precipitate with both potassium ferrocyanid and with phosphomolybdic acid. On heating, this extract gave a heavy coagulum at 74° C, but none above that tempera- ture, though the filtrate from the coagulum still gave a precipitate with both the i)otassium ferrocyanid and the i)hosphomolybdic acid. With hydrochloric acid the extract gave a heavy precipitate soluble in a large excess of the reagent. With cupric hydrate it gave a blue solution and a heavy preci[)itate. When the filtrate from the extract which had been coagulated by heat was evaporated somewhat and allowed to stand, several large colorless crystals resembling in form those of asparagin were obtained. These also gave the well-known reactions of that compound. Hence, it would seem that nitrogenous constituents of the arrowhead tubers consist of (1) a soluble casein-like albuminoid comprising nearly per cent of the dry tubers, (2) a soluble albumen coagulating at 74° C, (3) a small amount of an insoluble albuminoid of an unknown character, and (4) varying auiounts of nonalbuminoids of which asparagin is an important constituent. The total acjueous extract of the dry tubers (the second sample in the table) amounted to 29.18 i)er cent of the entire weight. Subtract- ing from this figure those corresponding to the soluble nitrogenous constituents, the soluble ash and the soluble carbohydrates, there remains 3.28 per cent of a soluble substance unaccounted for. The aqueous extract has a peculiar but not unpleasant odor. After coagu- lating the albuminoids, filtering and evaporating to dryness, there remained a brown sticky mass, very sweet in taste and having in a pro- nounced degree the peculiar odor of the extract itself. Hence, there is probably at least one unidentified substance which gives to the tubers their peculiar taste. This could not be obtained in a pure condition. The statement sometimes made that the arrowhead has acrid prop- erties apparently refers only to the leafy portion of the plant, or has arisen from a confusion with some other cultivated water plant. The foregoing facts seem to warrant the opinion that the arrowhead otters many advantages from a dietetic standpoint. When cooked in the same manner as potatoes, it forms, in the author's opinion, a very acceptable food, though it is not without a pronounced and characteristic flavor. It is stated that some of the poorer non-Mongolian residents of San Francisco have acquired a liking for it and use it freely. The util- ization of the arrowhead has already been commented on favorably by U. S. Dept. of Agr., Bui. 68, Office of Expt. Stations. o o © o o o o o ^(3 © 06 Plate 111. o 4 f » "* O " © © ( • © 6 » o O -o Q o Q Q Q Q) 6 Drawings of Starch Grains Magnified about 480 Diameters. 1, Sagittaria sine7isis; 2, Nehimbhim speciosum (roots); 3, Lilium juponicum brownii; 4, Trapa biapinosa; 5, Eurynle ferox; 6, Amorphophallus rivieri. 13 Pailleaux and Bois.* The tubers might also be used in the mauufact- ure of starch. They are employed for this purpose by the Chinese, and it is said a fine quality of that article is easily prepared from themi by the usual methods. The comparatively large areas of land both in California and in other parts of the United States which are too wet for the cultivation of other root crops would seem to offer an especial inducement to considering the utilization of this genius of plants. TARO. From remote antiquity the taro has furnished an important item of the food supply of the natives of southern India, Australia, portions of Africa, and many of the islands of the Pacific, and is to-day one of the plants most commonly cultivated throughout the Tropics. Botanically speaking, the taro cultivated for food may be any one of a number of species of the genus Colocasia (also known as Caladium), representatives of the family Aroidfie. The species commonly designated as Colocasia antiquorum, of which a large number of varieties are known, seems to be the one most widely cultivated, though G. indlca, C. odor a, and C. macrorhiza, the two former possibly only varieties of C. anUquorimi, are said to be extensively used. Most of these species are also used as ornamental greenhouse plants in temperate climates. Two forms of the taro are sold in large quantities in the Chinese market of San Francisco. Both are said to be imported either from Canton or from the Sandwich Islands. The first, which is the smaller form, is designated by the Chinese characters ^fj-, the second by 1^1115^. The two forms differ only in size, and produce plants which do not show even varietal differences. Evidently they are the roots of C. antiquorum. The smaller form consists of small ovoid or ellip- soid roots about 10 centimeters in length, which weigh from 60 to 70 grams. The larger roots are about 24 centimeters in length, and weigh from 500 to 800 grams. The latter form is considered superior to the former by the Chinese and is sold at a slightly higher price. The interior of the roots has about the consistency of a sweet potato, and a microscopical examination reveals the i)resence of large amounts of starch, which is present in the form of exceedingly small grains. The roots are easily started into growth, and the Chinese market furnishes a cheap source of supply of this well-known ornamental plant. Several analyses of species of Colocasia have been made by different investigators, most of which, however, are somewhat incomplete. In Table 2 are shown analyses of the two forms studied in this laboratory, one of a small and one of a large root. For purposes of comparison, an analysis by Kellner and one by Nagai and Murai are quoted : ' Soc. d'Acclimat., 4. ser., 5 (1888), p. 1102. 14 Table 2. — Composition of iaro. p ■£ g '3 n a is .a g -2 02 a P m a CS o bo a a m a o 00 o .9 a s a Oolocasia antiquorum (small root) : Original material ... Water-free snb- Per ct. 74.20 Per ct. 1.70 6.60 1.89 5.51 2.09 10.81 1.43 9.73 Per ct. 1.67 6.46 1.62 4.72 1.39 7.18 Per ct. 0.04 .14 .27 .79 .70 3.63 Per ct. 0.27 1.04 .16 .48 .17 .91 .08 .54 Per ct. 17.95 69.58 25.32 73.82 6.52 33.70 10.40 70.26 Per ct. 1.15 4.45 1.86 5.43 P.ct. .00 .00 .00 .00 P.ct. 0.98 3.78 .66 1.92 .70 3.63 p.ct. 1.31 5.10 1.10 3.21 .85 4.41 .90 6.69 P.ct. 2.44 9.45 Coloeasia antiquonim ' (large root) : Original material . . Water-free sub- 67.51 3.30 9 63 Oolocasia antiquorum: a Original material . . . Water-free sub- 80.65 15.54 80.24 Oolocasia antiquorum: b Original material . . . Water- free sub- 85.20 0.12 .81 1 78 12.02 a Reported by Kellner, Landw. Vera. Stat., 30 (1884), p. 42. h Reported by Nagai and Murai, quoted from Kiinig, Cbemie der menschlichen Nabrungs- und Ge- nussmlttel, 3. ed., I, p. 704. It is difficult to account for the remarkable difference between the starch content reported by Kellner and that reported in the three other analyses. Both the analyses reported from this laboratory were made in dux^licate, with substantially the same results. Kellner states that the roots contain large amounts of reducing- sugars. The extremely minute size of the starch grains present renders the separation of soluble from insoluble carbohydrates a very difficult matter. The water extract always showed a slight turl)idity, even after several flltrations, and it is possible that a considerable part of the substance reported as cane sugar was really starch in a very finely divided condition. The protein content shows a considerable variation in the four samples. In all cases it is rather low, but even in the two samples from San Francisco, which give the lowest results for this constituent, the amount present is not far from the average of the results obtained for potatoes and similar vegetables. The analyses also show tliat a large percentage of the crude protein is of an albuminoid nature, the proportion being somewhat greater than in the case of the potato. Most of this was found to consist of a soluble albumin which coagulates at 74° C. A red coloring matter is present in the taro in considerable amounts. As a whole the taro is characterized by the high percentage of car- bohydrates, of which starch is the most important representative, and by the low percentage of fat and crude fiber. From a dietetic stand- point it apparently offers no especial advantage over our commonly cultivated vegetables, but where it is eaten it seems to be a satisfactory substitute for them. It is favorably known to the Anglo-Saxon resi- dents of the Tropics, who soon acquire a liking for it, and even in San U. S. Dept. of Agi., Bui. 68, Office of Expt. Stations. Plate IV. Drawings of various Roots. 1, Portion of the rootstock of Nelumbiuni xpeciosuin aud cross section of same; 2, Tuber of Pachyrhizus angulatus; 3, The v. aX^^v chestnut (Eleochar is tuberosa); 4, A root of Blanihot, probably M. utilissima; 5, A small form of taro (Colocasia antiquorum). 15 Francisco there is a limited demand among the white races for the roots. A root of G. antiquoruni is shown in PI. IV, fig. 5. The area in the United States suited to the culture of taro is some- what limited. A patch of it has been growing in the Garden of Economic Plants of the University of California for a number of years, and has produced an abundance of large sound roots. In southern California it makes a rapid growth, but requires an abundant supply of water. The Florida Experiment Station ^ has also experimented with it and reports satisfactory results. WATER CHESTNUT. Though several species of Scirpus and the allied genus Eleocharis bear tuber-like roots, they are not commonly considered plants of economic importance by Europeans, and are seldom mentioned outside of the systematic manuals. The water chestnut {E. tuberosa) is said to be widely cultivated in China and Japan as a food plant, and is there highly esteemed. In the former countries it is known to the English- speaking residents as the water chestnut, which is but a free transla- tion of " ti leh," one of its Chinese names. Smith- says that the tubers are largely cultivated and sold for food all over China. "They grow wild in IIui)eh in watery places, and are not often especially planted. They are sweet, juicy, and resemble the cliestnut in flavor. * * * a kind of arrowroot |i. e., starch] is made from them." It has also been stated ' that Scirpiis fuberosus is cultivated in paddy lands for the sake of its tubers, which are eaten eitlier raw or boiled. Bretschneider'* states that it is cultivated all over China. The corms of the water chestnut, for they can scarcely be called tubers, begin to arrive in San Francisco markets in the latter part of May, and are to be found on sale throughout the remainder of the year. They are slightly flattened, about 5 centiuieters in diameter, and average about 13 grams in weight. They are surrounded by a rather thick cliestnut- brown skin, but within are nearly white and of a somewhat watery consistence. Under favorable conditions those purchased in San Fran- cisco will grow readily and produce an abundance of slender terete culms terminated in some instances by many-flowered spikes. Plate V shows one of these plants. In taste the corms are exceedingly sweet, and also i)ossess a peculiar but not unpleasant flavor. Their only resemblance to the chestnut is in the color of the outer skin. To the Chinese they are known as "ma hai," and are designated by the char- acters ,f^]Ej^, which difiter from any of those used by Bretschneider for • Florida Sta. Rpt. 1896, p. 9. 2 Contributions Toward the Materia Medica and Natural History of China, p. 92. Shanghai, 1871. ■' Useiul Plants of Japan, p. 27. Tokyo, 1895. "Jour. China Branch Roy. Asiatic Soc, 25 (1890-91), p. 47. 16 the plant. A drawing of three water chestnuts is shoirn in PI. IV, fig. 3. As far as observed no analysis of this vegetable has been published. The composition of two samples purchased at different seasons is recorded in Table 3. Table 3. — Composition of water chestnut. m c M "? 1 00 a o "2 'o a a to ^ ■g=s < ■^ .a 1 o a 00 ^3 a> .£3 .9 a -a > Ph <\ ^ M ^ <) t5 Eleocharis tuberosa (first sample) : Per ct. Per ct. Per ct. Per ct. P.ct. Per ct. Per ct. Per ct. p.ct. P.C«. P.ct. Original material. . . 77.29 1.53 1.16 0.37 0.15 7.34 6.35 1.94 0.94 1.19 3.28 W ater free s u b- stance 6.73 5.10 1.63 64 32.30 27.94 8.56 4.12 5.24 14.47 Eleocharis tuberosa (sec- oud sample) : Original material 77.89 1.31 1.00 .31 .27 8.09 6.02 2.60 1.22 1.18 1.42 Water- free sub- 5.91 4.54 1.37 1.23 36.58 27.23 11.78 5.53 5.32 6.42 Much difficulty was experienced in preparing the corms for analysis owing to the high percentage of soluble carbohydrates. This caused theni to form a sticky mass, rendering it impossible to reduce the sam- ple to a finely divided condition. Consequently the figures rei)orted for soluble carbohydrates in the analysis of the first samjile are proba- bly somewhat low, as is indicated by comparison with the analysis of the second sample in which this difficulty was largely overcome. On igniting the ash the green color, characteristic of manganese, was always observed. This color was also noted in a large number of the vegetable products grown in China.' The protein content of the water chestnut is rather low, being even lower than that obtained for the taro, but in respect to this con- stituent, it will still compare favorably with many of the vegetables in common use. Most of the protein consists of soluble albumin which coagulates at 70° C. The striking feature of both analyses reported is the high percentage of soluble carbohydrates. Presumably these con- sist very largely of cane sugar, though several attempts to prepare this in crystalline form from water extracts of bulbs were unsuccessful. As an article of food the roots, in the author's opinion, are very pal- atable. The high percentage of sugar gives them an agreeable flavor even while raw, in which state they are commonly used by the Chinese. But little information is accessible regarding the climatic conditions favorable to the development of the plant. The few specimens which were grown under glass seemed somewhat tender, but it is probable 'This would seem to indicate that too much reliance should not be placed on this test as a means of detoctinj; adulterations in teas. U. S. Dept. of Agr,, Bui- 68, Office of Expt. Stations. Plate V. Plant from a Corm of Eleocharls tuberosa. 17 that the moister regions of the Southern States or of the interior valleys of California would prove favorable to it. Experiments on this point seem desirable. SACRED LOTUS. This plant is better known from the pages of the early classic authors and from tradition than through modern works on economic botany. A plant of such surpassing beauty, whose habit of growth is so unique, could not fail to excite the admiration of all who were permitted to watch its development; and to the people of Egypt and many of the Asiatic countries it assumed the importance of a sacred emblem which was intimately associated with their religion and poetry. In addition to these more aesthetic uses, the plant has from time immemorial been one of economic importance and to-day occupies a not insignificant place in the domestic life of several nations. One of the most recent accounts of the plant, as viewed in its economic relations, is by Jules Grisard,' from which the following facts have been gleaned. The lotus (Kelumbium) is a native of western India, Persia, Cochin China, and perhaps of Australia, growing in ponds, stagnant water, and rivers. It is cultivated in the basin of the Mediterranean, where there is a summer temperature of about 21° C. In Cochin China it is cultivated in great vases j^laced at the doors of the houses. The uses of the lotus are numerous and varied. The stamens are employed in China as an astringent remedy and also for the toilet; the petioles and peduncles furnish a viscous sap employed in India as a remedy for vomiting and diarrhea; the fibro- vascular bundles of the petioles are made into lamp wicks and the carpophore furnishes a popu- lar remedy for blood spitting. The seeds contain a white starch used largely as food (see p. 39); roasted and ground they served the Egyp- tians for the manufacture of a kind of bread; in China they are used in soup and also as a remedy for indigestion. They are supposed to have invigorative properties when used as food by convalescents. The Chinese also extract from the root a starch which they say is very strengthening. A decoction of the rhizomes is used as a remedy for intestinal inflammation and the rhizomes themselves also become an important article of food in times of famine. Bretschueider'^ gives an interesting account of the references to the various parts of the plant in the Chinese classics, and Smith ^ gives many notes regarding its varied uses. The roots or, more strictly, rhizomes of the lotus are brought to San Francisco from Canton in considerable quantities and are on sale in ' Soc.'d'Acclimat., 1896, p. 189. 2 Jour. China Branch Roy. Asiatic Soc, 25 (1890), p. 216. ' Contributions Toward the Materia Medica and Natural History of China, p. 139. Shanghai, 1871. 3485— No. 68 2 18 tbe Chinese quarter throughout the late winter and early spiiiig months. As there found, the yellow nodular root stocks are often 100 centimeters in length, each internode measuring from 10 to 20 centimeters in length by about 7 in diameter. The interior is fleshy, but firm, and of a red- dish color. The roots are traversed from end to end by a series of ten or more large radially-arranged tubes, with a number of smaller ones between them. The appearance of one of these roots and of a cross section is shown in PI. IV, fig. 1. The roots contain an abundance of starch grains, which are oval, elliptical, or narrowly oblong, and exhibit a beautiful play of colors with polarized light and a selenite j>late. The stratifications are pronounced. In San Francisco the roots are known as "liu ngau," though the clas- sical name for the j)lant is " lien hua." The Chinese characters for them are j^l^. As far as could be learned, the roots are used in San Francisco for the preparation of a kind of " arrowroot" (i, e., starch), though sev- eral authors state that in China the roots are also boiled and used as a vegetable, or are eaten raw in somewhat the same manner as we use salad plants. It was found impossible to raise plants from any of these roots i)ur- chased in San Francisco. The terminal buds in most instances had been destroyed and the roots seemed to be lacking in vitality. There can be no doubt, however, that the roots in question were those of Nelumhinm speciosnm . In Table 4 are tabulated two analyses of these roots — one of a sami)le purchased in San Francisco, and the second, which is included for pur- poses of comparison, of Japanese roots N. nucifera [sjjeciosum] reported by Kellner. Tahle 4. — Composil'ton of loins roots. 03 . aura- Uim. These are sold at tlie rate of from 10 to 20 cents a pound. They are all imported from Canton. The bulbs have proved to be identical with the ones sold by our nurserymen under the name of 7v. hrownli, and this is a[)parently the only species sold by the Chinese merchants, as a large number of bulbs purchased at different times and from dif- ferent dealers have invariably yielded plants corresponding to this species. Unfortunately the bulV)s are often infested with mites which, either primarily or secondarily, cause the death of the plant before it perfects its flowers. From a collection of over 100 bulbs only 10 perfect tlowers were secured. The name L. hrownii seems to have been first i)ublishe(l by Poiteau,^ but has since been regarded as synonymous with L.japonicum*' or has been reduced to merely varietal significance. The latter disposition has been adopted here as being the most desirable from a cultural standpoint. What seems to be the same species may also be obtained in a dry form throngliout the year, and both this and the fresh bulbs are known under the name of " pak hop," and designated by the Chinese charac- ters "0^^. In Table "» the analyses of lily bulbs purchased in San Francisco are reported. One of them was of fresh the other of dried bulbs. Two analyses of Japanese- grown bulbs are also (pioted for purposes of com- parison. ' Araer. Nat., 16 (1882), p. 119. 2 Useful Plants of Japan. Tokyo, 1895. 'Jour. China Branch Roy. Asiatic Soc, 15 (1880), p. 179. ••Erythea, fi (1898), p.26. '■ Rev. Hoit., 2. ser., 2 ^1843-44), 496, ([uott'd from Index Kewensis, 3 (1894), p. 81. *• Baker, Revision of Tulipeie, 1875. 21 Table 5. — ComposUion of lihj hnlbs. 1 d 'S I a, "2 'S CI a J3 be a be IK b£i . a ■X (D o 13 (S a Lilium j aponicum brotvnii (dried bulbs) : Per et. Per et. Per ct. Per et. P.ct. Per et. Per ct. Per et. p.ct. P.ct. p.ct. Original material 10.16 5.57 5.00 0.57 0.37 62.65 2.84 0.00 1.64 2.68 14.09 Water- free substance 6.20 ,5.56 .64 .41 69.73 3.16 .00 1.82 2.98 15.68 Lilium japonicum brownii (fresli bulbs) : Orisiiial material 66.72 2.33 1.P5 .83 .59 17.74 4.16 .00 .75 1.24 6.42 Water free substance 7.01 4.50 2.51 1.78 53.40 12.51 .00 2.25 3.74 19.32 Lilium tigrinum: a -^ 71.46 4.51 15.79 .24 .84 <;21.60 C75.70 1.04 3.64 1.15 4.03 Lilivm sp. ("Yuri"):& 69.63 3.40 11.19 .11 .05 dlO.lO 62.83 1.42 4.66 1.35 4.44 Water- free substance a Reported by Kellner, quoted from Konig, Chemie der menschlichen Nahrungs- und Genassmittel, 3. ed., I, p. 704. ftlleported by Nagai and Murai, quoted from Konig, Chemie der menschlichen Nahrungs- und Genussmittel, 3. ed.. I. p. 705. c Carbohydrates by difference. d In addition the authors report in the fresh substance 0.62 per cent of glucose, 2.44 per cent of pectose, and 1.92 per cent of dextrin. Wide differences are to be noted between the composition of the two samples of bulbs purchased in San Francisco and the Japanese bulbs. This is not surprising when it is remembered that the bulbs were of different species and were grown in different countries. Tlie protein content is much smaller in the samples of L. brownii than in the others. In all the analyses the amount of protein is some- what above the average amount found in the i)otato. The percentage of albuminoids is noticeably greater than in the potato. Starch forms by far the most important constituent of tbe bulbs and is present in sufficient amount to warrant the belief that they have a high nutritive value (PI. Ill, fig. 3). The analysis quoted by Nitobe is interesting as giving somewhat definite information concerning the distribution of the carbohydrates, though he gives no statement as to the methods used in obtaining these results. Without them an accurate knowlei. campestris; Smith,^ Bretschneider, ' and apparently most writers on Chinese botany refer it to B. chinensis; Loureiro/ however, regards it as a form of his Sinajris peMnensis, to which he ultimately gave the varietal name of "pe-tsai," and Bailey has raised this name to a specific one. There are many varieties of "pe-tsai.'' The one sold in San Fran- cisco diflers from that figured by Bailey in the more densely compacted heads and in the greater spread of the leaf blades from the very base of the midrib. One head weighed 750 grams. When tested, this plant seemed to the author an entirely satisfactory vegetable. The composition of " pe tsai " is shown in Table 9, p. 32. The analysis there reported shows that this vegetable does not differ greatly as regards food values from our commonly cultivated cabbages. The water content is somewhat greater than the average of the figures for American-grown samples. The protein content is also somewhat lower, but nearly two-thirds of it is of an albuminoid character. The pres- ence of about 30 per cent, of reducing sugar, and also of considerable amounts of starch and small amounts of the cane members of the sugar group, is interesting, but corresponding determinations for other forms of cabbages were not found. B. chinensis^ anothel^ largely used cruciferous plant, is sold under the name of "pak-tsai," though the same Chinese character is used to des- ignate it as is used for the former vegetable. The samples found in the San Francisco market are made up into bundles, and consist of either the young and tender leaves or the blanched petioles of the leaves or the stalks derived from the central axis of the plant itself. The latter sometimes show the yellow mustard-like flowers, Bailey designates this plant, though with some hesitation, by the above name, but Forbes and Hemsley include it under B. c^nipestris. The comiiosition of "pak-tsai" is shown in Table 9, p. 32. In com- position this vegetable shows nearly the same features as the one \}ie- viously described exce{)t in the case of reducing sugars, which form only about 10 per cent of the dry material. The crude protein is also somewhat lower, but the figures for albuminoids are practically the same. On the whole, as regards com})ositi()n the vegetable is only slightly inferior to that ])reviously described, and seems Avorthy of a general trial. B. juncea. — Still a third cruciferous vegetable is found on sale throughout almost the entire year in San Francisco. It consists of green stalks often 50 centimeters in length, which are usually derived from ' Index FloriB Sinensis, 1886, p. 46. * Contributions Toward the Materia Medica and Natural History of China, p. 42. Shanghai, 1871. ^ Jour. China Branch Koy. Asiatic Soc., 15 (1880), ])p. 34, 124. ••Flora Cochinesis, 1790, p. 400. 29 the petioles only of B. juncea, though early in the season the entire leaf is used. This vegetable is known as "kiai tsai," designated by the Chinese characters ^^, and is probably a form of B. jimcea. Bret- schneider' reports that several varieties of Sinajyis juncea, or B.juncea, are included under the name "kiai tsai" at Peking, and gives the same character as is used in San Francisco for this vegetable. No analysis of "kiai tsai" was made in this laboratory, and none seems to have been reported by other investigators. This species often is sold in a dried form, and is also preserved in brine and used in the same way as sauerkraut. Amarantus sp. — The young seedling plants of Amarantus are in com- mon use as a pot herb among thi^ Chinese of the Pacific coast. J]oth our native species and a form which is said to be regularly cultivated are in use. The jilants found in market are too immature to admit of specific identification. This vegetable goes under the name of " in tsai," designated by the characters ^^, which Bretschneider- says is gen- erally applied to several species of Amarantus. Bailey^ describes a form of A. f/angeticus used by the Chinese as a pot herb. Smith ^ speaks of the use of ^. oleraceus and Forbes and Hemsley' state that A. blitum, A. caudatus, A. {/angeticus, and A. panicidata are commonly cultivated as vegetables in China. Several varieties of indigenous Amarantus and the closely related Cheuopodiums are eaten to a limited extent in the United States. Their value has been pointed out by Coville. •* The composition of Amarantus purchased in San Francisco is shown in Table 9, p. 32. The analysis here recorded presents no unusual features save in the high content of crude protein, in which feature it surpasses the cruciferous vegetables mentioned above. Solanum melongena. — A peculiar variety of the eggplant, known in San Francisco as "pak ke," designated by the characters g jjfi, is one of the noticeable features of the Chinese vegetable stands during the summer months. This has a perfectly smooth white skin and is from to 24 centimeters long and about 10 in diameter. Osbeck, Loureiro, and most of the early writers on Chinese botany speak of the cultivation of the eggplant. The composition of this eggplant is shown in Table 9, p. 32. Apparently but few analyses of this vegetable have been made, but the results obtained from the analysis of the Chinese variety in this laboratory agree quite closely with that of an American-grown specimen." The percentage of sugars and starch is apparently quite large, though the real nature of the carbohydrates is uncertain since 'Jour. China Branch Roy. Asiatic Soc, 15 (1880), p. 92. ^Ibicl., p. 168. 3New York Cornell Sta. Bnl. 67, p. 199. ^Contributions Toward the Materia Medica and Natural History of China, p. 12. Shanghai, 1871. ■'Index Floric Sinensis, 1891, p. 319. «U. S. Dept. Agr., Yearbook 1895, p. 210. '^U. S. Dept. Agr., Office of Experiment Stations Bui. 28, p. 38. 30 they were determined by difference. Starcli, however, «s shown by qualitative tests in this laboratory, is certainly present in considerable amounts. CUCURBITS. The fruits of several species of Cucurbitaceai are extensively used by the Chinese. Bailey' has already given an account of the cultural features and uses of four species, all of which were found on sale in San Francisco and are there used in large quantities. Further infor- mation concerning these vegetables is given by Lander-* and by Georgesou.'' Momordica charantia, the "fu kwa" or "khu qua" of Bretschneider, or the "la qua" of Bailey, is designated by the Chinese characters '^ 1^. This vegetable is noted in nearly all works descriptive of the botany of China, where it is largely cultivated and to which country it is probably indigenous. It is also sometimes called "lai kua," or the lei)rosy gourd. Its immature fruit is largely used throughout the Trojjics as a condi- ment in the j)reparation of curries, etc. By the Chinese it is used espe- cially in the preparation of salads, etc. The closely related M. haJsamina is already somewhat widely known in America as an ornamental vine, and the seeds of both species are to be obtained from American seeds- men. The shuttle-shaped green fruits of M. clmraniia are about 2 deci- meters in length, covered with rows of wart-like tubercles, and are borne on wiry stems of about the same length. Like all the other green vegetables described above, they are grown in large quantities in the Chinese gardens along the Sacramento River. The M. eharanfia can scarcely be regarded as a food i>lant in the nar- rower sense of that term, as the i)rincipal value of its fruits depends upon the flavor they impart to other preparations, rather than on the amount of nutriment they contain. Nevertheless, large amounts of the fruits are eaten, and they must be considered in studying the dietary of the Chinese. The analysis here reported (Table 9, p. 32) shows that the fruit is not lacking in nutritive constituents, though the exact nature of the compounds represented could not be determined without a more extended investigation. The crude fat in this case apparently consisted almost wholly of chlorophyll. Of the 18 per cent of nitrogenous con- stituents, about two-thirds was of an albuminoid nature. Keducing sugars, or at least soluble carbohydrates having reducing ])0wer, were found to be present in con sidei able quantities, and true starch was also present in relatively large amounts. Luffa acxtangula, is known as "sz kwa," and both this and the fol- lowing species are designated by the Chinese characters ^^^J^. This is a native of tropical Asia, though naturalized in certain regions of America. The name above given is synonymous with L. fcetida and I New York Cornell Sta. Bui. 67, pp. 191-196. •''Garden and Forest, 1 (1888), p. 483. :' Amer. Garden., 13 (1892), p. 526. 31 with Cucuniis acutangulus. The plant produces a green, pronouncedly ten-ribbed fruit, about 3 decimeters in length, which is obtusely termi- nated at the farther end, but tapers gradually to the point of union with the peduncle. In uses and culinary qualities it much resembles the common cucumber. In San Francisco, it is said, the Chinese use it for thickening soups. Bailey' states that it is eaten raw, and also cooked in the same manner as we use squashes. As shown by analysis (Table 9, p. 32), it does not difter greatly from the species previously described, the chief difference being in the lower percentage of nitrog- enous substances and in the greater content of carbohydrates, espe- cially of reducing sugars. Liiffa cylindrica is called by the Chinese "po kua." This is the "sua kwa" of Bailey.- This plant is also widely cultivated throughout the Tropics, but its native country is unknown. It is synonymous with L. (vgyptica, L. petiola, and Momordica cylindrica. It is reported from China by most of the naturalists who visited that country in early days, as well as by thB more recent botanists. Its slender, (;ylindrical, crooked, yellowish-green fruits, which are often decimeters long, are used in the immature condition in the same manner as we use squashes. The interior of the mature fruit is filled with a fibrous mass, which when dried forms a useful household article. Hence the plant is often kno\\n as the ''dish-cloth" or " towel gourd," and is more often grown for its fibrous interior than as a food. In San Francisco, however, it is largely used for the hitter i^urpose, being prepared in much the same manner as squashes are treated in American households. As shown by analysis (Table 9, p. 32), it is on the whole about equivalent in food value to the previously-described species, the protein content being somewhat less, while the percentage of reducing sugars and starch is somewhat greater. As shown by comparison, it is rather inferior to the common varieties of squash, and it can scarcely be regarded as more than a passable substitute for them. Benincasa cerifera. — This plant is known as "zit kwa," and is desig- nated by the characters fp JJS^. It is the same as the "tung kua" of Bretschneider,' designated by the characters :^jri5;^. Under the name of "Chinese preserving melon," it is already somewhat known in the United States, It is a tropical vine, long cultivated in China, Japan, India, and Africa, where it is often met with growing spontaneously, though its native country is unknown. The young green hairy fruits when about 20 centimeters in length, are used in the same manner as we use the squash. The mature fruits often attain a weight of 12 kilograms, are perfectly smooth, and covered with a white wax. With the exception of a small amount of pulpy substance which is filled with the white seeds, the interior is made up of a white solid fiesh. The > New York Cornell Sta. Bill. 67, p. 196. 2 Ibid., p. 195. 3 Jour. China Branch Roy. Asiatic Soc, 15 (1890-91), p. 153, 32 mature fruit is used by the Chinese in the preparation opf confections and is also said to be used as a vegetable. The analysis reported below presents no especially interesting features save the high per- centage of reducing sugars. The composition of the green vegetables and cucurbits discussed above is shown in the following table: Table 9. — Composition of green vegetables and cucurbits. GREEN VEfiETABLES. Brassiea pe-Uai: Per ct. Original material 95.74 Water-l'ree substance Brassica chinciisis: Original material 96. 55 Water-free substance Amarantus sp. : Original material 91.52 Water-free substance Solanum ■)nelon<)ena: Original material... 89.62 Water- free substance CDCUEBITS. Momordica charantia: Original material 93.61 Water-free substance Liiffa cylindrica: Original material 94.66 Water- freesubstance Luffa acutanguln: j Original material 94.90 Water-free 8iib.stance , Benincasa cerifera: j Original material [ 96. 24 Water free substance Perct. 1.19 28.07 .78 21.96 2.61 30.80 1.38 13.25 1.18 18.48 .51 9.57 13.39 ..50 13.27 Per ct. 0.48 11.36 .41 11.43 1.67 19.68 1.08 10.42 .79 12.31 .54 10.68 Per ct. 0.71 16. 71 .37 10.53 .94 11.12 .29 2.83 .39 6.18 .13 2.50 .14 2.71 -a 1 C3 3 a o te . .S£ 3 1 D 3 O < P.ct. Per ct. V. ct. Per ct. Per ct. P.ct. 0.15 0.31 0.09 1.29 0.52 0.56 3.57 7.19 2.11 30.25 12.16 13. 28 .10 .31 .09 ■ .37 .46 .65 2.82 8.61 2.45 10.45 12.86 18.33 .36 .50 5.82 1.57 .92 10.81 1.54 1.56 18.31 .69 4.26 .30 .63 1.31 2.91 15.11 6.10 12.66 14.88 6.69 .yo .67 .06 .60 1.07 .34 3.19 10.56 .74 9.36 16.72 5.25 .10 1.04 .12 2.15 .46 .41 3.72 19.52 2.18 40.29 8.58 7.65 .24 .36 .10 1.57 .72 .43 4.70 7.03 1.95 30.86 14.03 8.43 .16 .31 .07 .90 .57 .35 4.34 8.29 1.74 24.19 15.19 9.48 P.ct. 0.14 3.38 .80 22.52 2.55 30.00 2.95 28.39 2.28 35.69 .45 8.40 1.00 19.61 23.49 SEEDS AND GRAINS. SOY BEANS. Leguminous seeds and certain preparations made from them have always formed an important part of the largely vegetarian diet of the Chinese and Japanese. Of the legumes the soy bean, Glycine Mspida {iSoja hifipida), is the most important. The soy bean has long been known in Europe. Kaempfer' was perhaps one of the first i'hiropeans to describe it. This plant has been cultivated many years in Europe, and is coming to be quite extensively grown in the United States, largely for use as a forage plant. The soy bean has been treated of in a previous publication- of this Department. A large number of vari- eties of the soy bean are in cultivation in China and Japan, but only two were found in the Chinese markets in San Francisco, a yellow and a black variety. Aside from a difference in color, the two forms 1 Amoinitatum Exoticarum, p. 837. Lemgovise, H. W. Meyer, 1712. * U. S. Dept. Agr,, Farmers' BuUetiu 58. U. S. Dept. of Agr., Bui. 68, Office of Expt. Stations. Plate VI. Upper Portion of a Plant of the Black Soy Bean. 33 apparently do not differ materially from eacli other. Tlie yellow vari- ety is known as "woiig tau," and is designated by the characters ^j^, while the black is known as ^'hak tau," and is designated by the charac- ters Mj^. The soy bean resembles a pea rather than a bean, although the botanical characteristics of the plant indicate that it is very differ- ent from any of our cultivated peas or beans. Both varieties obtained from the Chinese market in San Francisco grew readily in Berkelej^, attaining a height of about 3 feet, and in spite of a very dry season produced an abundant crop of seeds. The appearance of two of these plants at different stages of growth is shown in Pis. VI and VII. The composition of the seeds of the two varieties is shown in Ta,ble 10, the average composition of American- grown soy beans being quoted also for purposes of comparison. Taule 10. — CompoMion uf soy beans. Hi in -d n3 -s . C s ^ a C sr. & M . g n 'm ^ s ■S £ « s .d Sf §^ a o 8=^ < 4i> a •a 3 ^ ^ < pq M 5 (S n o < a Glycine hispida (black) : Per ct. Per ct. Per ct. Per ct. Per ct. Per ct. Per ct. P.ct. p. ct. P.ct. P ct. Original material 8.25 36.35 34.63 1.72 17.22 6.80 7.38 0.00 5.25 4.77 13.98 Water- free substance 39. 62 37.74 1.82 18.77 7.41 8.04 0.00 5.72 5.20 15.25 Glycine hispida, (yellow): Original material 8.33 36.00 35.54 .46 17.87 5.87 6.55 0.00 5.43 4.75 15.20 VV atert ree substance Glycine hispida (average 39. 27 38.77 .50 19.49 6.40 7.14 0.00 5.92 5.18 16.59 " ot 8 analyses) -.a 10.80 33.98 38.1 16.85 19.00 h 28. 89 4.79 5.4 4.69 5.3 a U. S. Dept. Agr., Office of Experiment Stations Bui. 11, p. 120. h Carbohydrates by ditt'erence. The analyses of these two Chinese varieties of soy beans, as well as of others which have been published, show that they contain an unusu- ally large amount of valuable food constituents, especially protein and fat, the former constituting about 39 and the latter about 19 per cent of the total weight. As shown by the analysis made by the author, nearly all the protein is made up of albuminoids. The figures obtained by the author for cane sugar and starch indicate that a considerable amount of carbohydrates was present, although starch could not be detected by the ordinary test. No special attempt was made to sepa- rate the cane sugar. There has been considerable difference of opinion among investiga- tors regarding the presence of starch in soy beans. The weight of evidence, however, seems to indicate that, under certain conditions, starch in considerable amounts may be present. In 1880 Meissl and Bocker investigated soy beans and reported the presence of starch. The amount of starch found was said to be less than 3 per cent. The 1 Sitzber. Math. Naturw. CI. Akad. Wisa. (Vienna), 87 (1883), pt. 1, pp. 372-391. 3485— No. 68 3 34 starch grains are described as being extremely small and as flifferingiu form from the typical bean or pea starch. About the same time Han- ausek ' also reported starch in soy beans. It was found deposited where the surface of the cotyledons met, and according to the author, could not be detected by the usual chemical methods. The starch grains were embedded in fat and gave no color reaction after prolonged treatment with iodin. A more extended study of soy beans was reported by Harz.- According to this author, when the beans do not ripen thoroughlj , or when allowed to ripen after the vines are cut, they may contain starch, some varieties being more likely to contain it than others. luoyue ^ also was unable to find starch in the mature seeds. The carbohydrates other than starch in soy beans have been studied by many investigators. Stingl and Morawski ^ report the presence of small quantities of dextrin in soy beans, and about 2 per cent of a mixture of different sugars which could be readily fermented. Their investigation was principally concerned with the diastatic ferment found in the beans. A number of tests on the quantity of the ferment are reported. La Yellois'' reported the presence of 9 to 11 j^er cent' of material soluble in alcohol, which did not reduce Fehling's solution. Tollens^ calls this substance galactan. Schultz and Frankfurt' proved the presence of cane sugar in soy beans. Maxwell ° identified paraga- lactan, an insoluble carbohydrate. A number of other investigations on the carbohydrates of soy beans have been reported. In 1880 Meissl and Bocker^ reported an extended investigation of the proteids of soy beans. According to these investigators the beans contained the following proteids: A so-called "casein" (27.G per cent), albumen (0.5 per cent), a proteid precipitated by cupric oxid and potas- sium hydroxid (2.5 per cent), and, in addition, a very small amount of nonalbuminoid nitrogenous substance. Osborne and Campbell'*' have recently reported an extended study of the proteids of soy beans. The principal proteid found is called "glycinin." It is a globulin. Accord- ing to these authors the beans also contain about 1.5 per cent of an albumin-like proteid, legumelin. A small amount of proteose was also found. Meissl and Booker^ studied the fat of soy beans, which is said to con- tain no free fatty acid and to consist almost entirely of neutral trigly- ' Ztschr. AWg. Oester, Apoth. Ver., 22 (1884), p. 474. "Ztschr, Allg. Oester. Apoth. Ver., 23 (1885), p. 40; Landwirtschaftliclio Samen- kimde, p. 692. Berlin, P. Parey, 1885. 'Imp. Univ. Col. Agr. [Tokyo] Bui., Vol. 2, No. 4. ■"Monatsh. Cliem., 7 (1886), pp. 176-190. '^Compt. Rend. Acad. Sci. Paris, 93 (1881), p. 281. '■ Handbuch dor Kohlenhydraten, vol. 1, p. 208. Breslau, E. Trewoiidt, 1888. ■ Bar. Deut. Chem. Geseli., 27 (1894), p. 62. » Amcr. Chem. Jour., 12 (1890), p. 51-60. 'I.oc. cit. "Conn. State Sta. Rpt. 1897, pt. 4, pp. .374-382. U. S. Dept. of Agr., Bui. 68, Office of Expt. Stations. Plate VII. Mature Plant of Yellow Soy Bean. 35 cerids. At a low temperature or on standing a long time the palmatin and stearin triglycerids were precipitated in crystalline form. The fat has a characteristic leguminous taste. When kept for two years it became thick, but was only slightly rancid. Its specific gravity was 0.89 at 150 C. One gram of soy-bean fat required 191.S milligrams potassium hydroxid for saponification. The fat has also been examined by Stingl and Morawski' and by Eoelofsen^ with reference to its iodin absorption. The figures obtained for the latter constant were 121.3 and 138.8, respectively, and these and other facts indicate that the oil has poor drying properties. Pellet ' studied the ash of soy beans in considerable detail. Soy beans are eaten to some extent when cooked in the same ways as other beans. The Chinese express an oil from them which is a standard article of commerce and is largely used in cooking. The principal use which they make of the soy beans, however, is in the preparation of a vegetable cheese, a kind of thick sauce, and other prod- ucts. An account of the manufacture of miso and soy-bean sauce in the early part of the eighteenth century is given by Kaempfer.^ According toPrinsen-Geerligs,^ " tao hu," or bean cheese, is jirepared froin the seeds of the white variety of soy bean. These are allowed to soak for three hours in water, are then reduced to a thick paste, and the mass cooked. The cooked mass is strained through a coarse cloth. The filtrate con- sists of a milky- white liquid containing protein and fat. As soon as this becomes cool some material is added (for instance, crude salt con- taining magnesium chlorid), which precipitates the proteid material, the fat being inclosed in the coagulated mass. The coagulated material is pressed and kneaded into small cakes. The cakes may be dipped for a few moments into a saline solution of curcuma. Variations in the process give rise to a number of varieties of bean cheese. This is essentially the method used by the Chinese of San Francisco in the preparation of the bean cheese used by them. It is sold either in the form of the freshly precipitated curd or in the form of small square cakes obtained by comj)ressing the former material. It is usually cooked in peanut oil before being eaten and, in the author's opinion, is a palatable food. A partial analysis of one of these cakes gave water 81.35 per cent, fat 5.19 per cent, and ash 0.80 per cent. The filtrate from the cooked soy beans resembles milk, and, on heat- ing, a skin, not unlike that formed on milk, rises to the surface of it. A thick sauce called "tao yu," resembling the Japanese "shoju," is also prei)ared from the soy beans, as well as a thick condiment called "tao tjiung," similar to the Japanese "miso." According to Prinsen- Geerligs, these preparations have the following composition : 'Chem. Ztg., 10 (1886), p. 140. 2 Auier. Chem. Jour., 16 (1894), p. 49. 3 Compt. Rend. Acad. Sci. Paris, 90 (1890), pp. 1177-1180. ^Loc. cit. 6 Chem. Ztg., 20 (1896), pp. 67-69. 36 Table 11. — Composition of Chinese soy-bean preparations. Tao hu (bean cheese) Milk from boiled soy beans. Tao yu (soy sauce) Tao tjiung" "Water. Protein. Percent. 76.15 93.10 57.12 62.86 Percent. la. 15 3.13 7.49 12.67 ^ ^ Carbohy- ^**- ' drates. Per cent. 7.09 1.89 Per cent. 1.40 16.03 6 13. 78 Ash. I TTndeter- ! mined. Per cent. 2.21 0.51 a 18. 76 6.71 a Including 17.11 .sodium chlorid. blncludini; 3.78 crude fiber. The Japanese preparation made from soy beans, similar to those mentioned above, have been described by a number of investigators. A brief account of theiu has been given in a previous publication of this Department.' As shown by their composition, these soy-bean prepara- tions contain a high percentage of nutrients. Tliey are eaten in large quantities and form important articles of diet. A number of diges- tion experiments have been reported in which the Japanese ])repara- tions formed a considerable part of the diet, and on the basis of the results obtained the preparations are considered to be very well assimilated. PHASEOLUS. The seeds of two varieties of Phascoins mnngo are largely used by the Chinese in San Francisco. One of these, known as "iuk tau," designated by the characters |^_a^, is a small green bean. The individ- ual seeds weigh only about 0.044 gram. They are slightly flattened at the ends and have a rather long hilum. Plants were grown at Berkeley, Cal., from these seeds and yielded a good return, though the plant was not as prolific as the soy bean. The appearance of one of them is shown in PI. VIII. The analyses in the table below show that these beans differ in composition but slightly from the commonly cultivated varieties of P. vnh/aris. The Chinese use these beans largely in the preparation of "bean sprouts," which are simply seeds that have been soaked in water and allowed to germinate till the young plants are severaliuches in length. This product is said to be used in making soup. It is difficult to imagine what advantage the young plants possess over the original seeds for such a ])urpose, unless they impart a different flavor. From an economic standpoint the process is wasteful, as it involves the trans- formation of albuminoids into various cleavage products and amido- compounds, whose nutritive value is thought to be but slight. Henry^ refers to the preparation of a kind of vermicelli from this bean, but such a i)reparation was unknown in San Francisco. The second form of Phaseolus in use Is of a dull red color, rather larger than the previous variety, and more nearly spherical in shape. This is known as the "huang tau" or red bean, designated by the characters -fj^. Kelluer analyzed the seeds of a variety of P. radiatus ' U. S. Dept. Agr., Farmers' Bui. 58, Appendix. * Notes on the Economic Botany of China, p. 13, Shanghai, 1893. U. S. Dept. of Agr,, Bui. 68. Office of Expt. Stations. Plate VIII. Plant of Phaseolus mungo. 37 grown in Japan, wliicli is very probably the same as the one here described, as the two species are by some authors regarded as identical. Harrison and Jenmau report the analysis of mungo beans grown in British Guiana. The composition of the red and green P. mungo is shown in Table 12. For purposes of comparison the composition of a green form of P. mungo reported by Church, P. radiaitis' reported by Kellner, and "mungo" beans reported by Harrison and Jenman, are also given. Tablk 12. — Composition of seed of Phaseolus iipp. Phnseolus mungo {green) : Original materia/ Water- free substance Fhaseolus mungo (red) : Original iii;iterial Wat er-lree substance Ph aneolus mungo (ureen) : a Oriiiiiial material "Water-freo substance Phaseolus radiatut (red) :c Original material Water-free substance "Mungo beans:" e Original material Water- free substance Per ct. 8.83 Per ct. 22.64 24.83 21.06 23.52 22.2 24.9 18.30 20.84 » ■:^ c ■= 2 c X S ^ c*^ ■< < Per ct. Perct. 21.88 0.76 24.00 .83 18.19 20.32 2.87 3.20 16.74 19.06 20.54 24.09 1.56 1.78 1.52 1.78 Per ct. 1.34 1.47 2.7 3.0 1.42 1.62 1.96 2.29 Per ct. 48.54 53.23 48.36 54.02 57.40 65.38 /6.71 7.87 P.ct. 0.00 .00 .00 .00 6 54.1 b60.7 d2. 02 (?2. 31 P.ct. 4.52 4.95 5.02 5.61 5.8 6.5 3.74 4.38 P. 2.60 2.96 3.39 3.98 P.ct. 11.28 11.39 9.61 10.73 aEeported by Churcli, Pood Grains of India. London, Chapman and Hall, 1886, p. 151. 6 Carbohydrates by difl'erence. cReported by Kellner, Landw. Vers. Stat., 30 (1881), p. 42. d Determined by ditiereuce. e Reported by Harrison and Jenman, Rpt. A jr. Work Bot. Gard. British Guiana, 1891-92, p. 73. /The authors also report in the fresh substance 41.23 per cent digestible liber and 1.80 per cent pec- tose, gums, etc. Harrison and Jenman^ also report analyses of a number of beans whose botanical names were not known. They were called red " mote," "Chinese bean," '' kingto," and " octow." The results of Kellner's analysis, as well as those secured at San Francisco (the red Phaseolus mungo), show that the two varieties have essentially the same composition. The analyses also show that about 90 jjer cent of the protein is in the form of albuminoids. Osborne^ has studied the proteids of the white-podded adzuki bean, which is presumably the same variety as the one analyzed by Kellner. He found they consisted of phaseolin and a hitherto unknown globulin. DOLICHOS. Still another bean, which is largely used by the Chinese of San Fran- cisco, is Dolirlios sesquipedaUs. These seeds are white, with a promi- nent black ring which surrounds the hilum. The beans weigh about 0.31 gram each. The skin covering the seed is especially tough, but ' Loc. cit. 2 Jour. Amer. Cheui. Soc, 19 (1897), p. 509. 38 can readily be removed after soaking in water. The beans are most largely used in the form of " bean sprouts " after the removal of this skin. They are known as ''mi tau" (/g ji); but this name and its accompanying characters have not been found in any of the books on Chinese botany now accessible to the author. The plant is said to be a native of South America, and was probably recently introduced into China. As shown below, the composition of the dry bean differs but slightly from that of our commonly cultivated variety of beans. The green pods of this species are also largely used as a snap bean. These are from 50 to 70 centimeters in length, and contain from 10 to 16 seeds. They are more slender than most of our string beans, and slightly ridged along the middle of the two valves. This vegetable is known as " tou kok," and is cultivated by the Chinese along the Sac- ramento Eiver, arriving in market early in July. It is said that it has come into use among the white residents of the central portion of Cali- fornia, and is considered a valuable variety of string bean. The plant is somewhat tender, and requires a long season for development, but Bailey ^ reports fair success with it in the eastern United States. Table 13 shows the composition of the seeds and green pods of D. sesquipedalis as compared with that of D. lablab, cowpeas, and other beans. Table 13. — Composition of Doliclios and other heans. S o 2 '3 a a .=1 .a o 1^ i i fcJC s M . 'S ej u g a < a a Dolichog sesqnipedalis (seed) : Per ct. Original mat eriaL... 10.98 Water-free substance: Per ct. 22. U 25.54 24.4 27.8 21.4 24.6 22.5 25.74 4.54 22.63 2.3 21. 30 Per ct. 17.84 20. 04 P.ct. 4.90 5.50 P.ct. 2.66 2.99 1.5 1.7 1.4 1.61 1.8 2.07 .53 2.04 .3 2.77 Per ct. 45.91 51.57 Per ct. 4.27 4.80 Per ct. 0.00 .00 Per ct. 2.80 3.15 1.2 1.4 4.1 4.71 4.4 5.03 2.56 12.74 1.9 17.59 P.ct. 2.86 3.21 3.4 ?.9 3.4 3.91 3.5 4.0 1.17 5.81 .8 7.41 P. Ct. 7.78 8.74 Doliclios lahlah (seed) -.a Original material 12. 1 &57.8 6 65. 8 6 56.7 b 65. 17 h 55. 2 b 63. 16 Cowpeas ( Vigna catjang) (Average of 13 analy- ses) :C 13.0 12.6 79.92 SB 9 Dried beans (average of 11 analyses) :d 3.04 15.14 1.50 7.49 Dolichos sesqvipedalit (green pods) : ( )riginal material Water- free substance 2.74 13. 66 1.75 8.72 3.26 16.24 3.53 17. 56 String beans (average of 5 analyses) : d 6 5.5 b 50. 93 a Reported by Church, Food Grains of India. London: Chapman and Hall, 1886, p. 161. b (,'arbohydrates by ditierence. c r. S. Uept. Agr., (XHce of Experiment Stations Bui. 28 (rev. ed.), p. 37. ri Ibid., p. 65. Harrison and Jenman ^ report the composition of two varieties of D. lahlah, the crinkled-podded Bouavist bean and the flat-podded ' New York Cornell Sta. Bui. 67, p. 197. -Rpt. Agr. Work Bot. Gard. British Guiana, 1891-92, p. 70. 39 Boiuivist bean. Their results are not quoted, since the beans were evidently a different species from those purchased in San Francisco. Both fresh and dry, the Bolichos analyzed in this laboratory compares favorably with the ordinary American beans, and were found on trial to be a satisfactory substitute for these, provided only the tough skin was removed from the dry seeds, which was readily effected. This species seems worthy of a general trial. VARIOUS WATER PLANTS. Nelumhium speciosum. — In reviewing the numerous uses of the sacred lotus (p. 17), it was stated that the seeds were an article of food among Asiatic nations, and it is not surprising to tind them on sale in San Francisco. Their peculiar shape, the remarkably mature condition of their embryos, and the peculiar form of the embryo itself, all render the seed easy of recognition. Two forms were obtained, the one being somewhat larger and more irregular in shajie than the other, but it was impossible to ascertain whether they were the product of plants which differed materially from each other. The smaller and more commonly used of the two forms is known as "pak lin" and is designated by the characters j^j^; the larger is known as " seung lin" ('^j||). In all instances the thick outer coating of the seed on sale in San Francisco had been removed, leaving only the inner kernel. Under favorable conditions of temjierature and moisture the seeds purchased germinated rapidly, and in the course of a few months from the time of starting pro- duced thrifty plants. Thus far the i:>lants from the two varieties of seeds do not diner materially from each other nor from the commonly cultivated form of lotus. As yet none have produced flowers. The germination of the seeds is somewhat interesting from a botanical point of view and is figured in PI. II, fig. 4. The seeds are eaten by the Chinese either raw, boiled, or roasted, being used as we use chestnuts. They are also said to be used in soup, though this seems to be a general term used to designate any mixture that has been boiled. The author w-as also assured that a form of "arrowroot," i. e., starch, is made from the seed. The dark green germ is decidedly bitter and must be removed before the seed becomes j^alatable. Its bitterness has given rise to the Chinese saying, "bitter as the plumule of the lotus seed." Analyses of the two forms are given in Table 14, p. 41. In the sam- ple of large seed the germs, which form 1.3 per cent of the weight of the entire seed, were removed before analysis, but in the case of the smaller form the entire seed was analyzed. An inspection of the two analyses shows that the seeds contain a high percentage of nutrients. The protein content is considerably above that of the commonly cultivated cereals, but does not approach that of the leguminous seeds. Nearly all of it is of an albuminoid nature. As might have been expected, starch is the most important nonuitrogeuous constituent, though small amounts of other carbohy- 40 drates are also present. The starch found in both varietiea* differs in form from that found in the roots of the lotus. Uuryaleferox. — Tins is another aquatic, closely related to the lotus both in habit of ^ixowth and in botanical aftiuities. It is designated by the characters -^i^. Lite the lotus its seeds form a part of the com- plex dietary of the Asiatic races.^ These seeds were occasionally met with in the Chinese stores, but only in a broken and imperfect condition, apparently the result of a mechanical process intended to crack them open. Xevertheless the broken seeds showed such a remarkable resem- blance to those of our native l^iqiliar pohjsepalum^ which also furnishes seeds used by the Klamath Indians of Oregon- for food, that the author was at once led to suspect their relationship, and a dictionary in the Cantonese dialect, by John Eitel, associates the character used for them with the name given. The seeds are much smaller than those of the lotus, more larinaceous, and their embryos show only a moderate degree of development. The characters used to designate them are quite different from those used for the lotus seeds and the name commonly used is '-tsz shat," which, however, does not appear in any of the works descriptive of Chinese botany accessible to the author. They are used principally as an addition to soup, in much the same manner as we use barley, but are also said to be used for the manufacture of starch. The accompanying analysis (Table 14, p. 41) shows tliat they possess a food value about equivalent to that of the cereals, the principal con- stituent being starch, whicli is present in the form of exceedingly small grains, as shown in PI. Ill, fig. 5. Sugars are probably not present, as the small amount reported in the analysis may represent traces of starch which repeated filtration failed to remove from the aqueous extract of the seeds. But little information is accessible regarding adaptability of the plant to the conditions found in the United States. It is already in cultivation in the North Atlantic States and other regions as an ornamental aquatic. Trapa hispinosa. — The curiously-shaped seeds of this plant, which may be aptly compared with the head and horns of a cow, are familiar objects in the Chinese bazaars, where they are sold as curiosities. The regular Chinese merchant, however, is quite sure to have them in stock during the spring mouths, and they are sold by him as an article of diet. In the bazaars they are known as the "horn chestnut." The Chinese name is "ling ko" (^H). This is also the name by which they are known in the Chinese classics, as the seeds are sometimes included among the five food grains of China. The seeds do not keep well, and those which find their way into the bazaars are often entirely decayed within. The fresh seeds have a kernel which, in consistency and taste, resemble the chestnut. They will germinate readily if placed in a vessel of water ' Bretscluieider, Jour. China Branch Roy. Asiatic. Soc, 25 (18 90), p. 218. •^Coville, Contrib. U. S. Nat. Herb., 5 (1897), No. 2, p. 96. 41 and i^roduce the curious floating foliage characteristic of this genus of plants. (See PI. II, tig. 1, and PI. Ill, tig. 4.) Authors are at variance as to the correct name of the plant produc- ing these seeds. Forbes and Hemsley ' regard it as one of the forms of the more widely known European T. natans inasmuch as they find many intermediate forms between the Cliinese and the European species. By other writers the plant is variously designated as T, hlspinosa^ T. bicornis, T. cochinchinensis, and T. incisa. The plants to which these terms are applied are probably identical. As a means of distinguish- ing it from T. natans, from which the seeds analyzed by the author certainly ditfer, the name T. hisphiosa has been adopted in this report. The composition of the two sorts of Nelurabium seed, of Euryaleferox, and Trajm hispinosa are shown in the following table: Table 14. — Composition of seeds of various water plants. 1 Protein. Albuminoids. -Si ^ .a .2 2 IS 1 •6 .a 5 Nelumhium speciosum (large form) : Original material . . . Water-free substance Nelutnbium gj^eciosum (smaller form) : Original material ... Water-free s ubstance Euryaleferox: Original material . . . 'Water-free .substance Trapa bispinofa: Original material . . . Water- free substance Per ct. 8.72 9.40 13.10 10.59 Peret. P»ret. 16.64 : 15.47 18.23 16.95 17. 73 17. 64 19.57 19.46 9. 79 • 9. 79 11.26 11.26 10.88 10.42 12. 16 1 11. 65 Perct. 1.17 1.28 .09 .10 .46 .51 Per ct. 2.44 2.67 2.96 3.26 4.90 5.64 .65 .73 Perct. 51.64 56.57 40.63 44.84 68.07 78.33 60.39 67.53 Perct. 4.09 4.48 9. .55 10.54 1.59 1.83 P.ct. 2.41 2.64 3.95 4.42 P. ct. 3.15 3.45 2.95 3.26 .83 .96 1.41 1.58 P.ct. 3.03 3.32 4.15 4.58 .54 ,62 2.57 2.87 p.ct. 7.88 8.63 12.63 13.94 1.18 1.36 9.56 10.70 The analysis here reported of the seeds of Trapa hispinosa is not typical as regards water content, since the seeds used had been kept for a considerable time before they were analyzed and were unusually dry. The average weight of the seeds is about 4 grams and in those used the kernel constituted about 64.3 per cent of the total. Their food value is slightly less than that of the seeds of the Euryale, but, judged solely by their composition, they nevertheless contain a high percentage of nutritive material. CHINESE MILLET. Though this grain was not found on sale in San Francisco, it has a widely extended use in China and Japan. The sample analyzed in this laboratory was given to the author by Professor Fryer, who states that it is more especially used in the northern part of China. He considers it a very valuable article of diet and believes this plant should be intro- duced into cultivation in the United States. The species (presumably Paniciim) to which the sample belongs can not be determined until ' Index Flora} Sinensis, 1887, p. 311. 42 plants have been grown from it. Several species of Pgftiicnm and closelj" related genera are in cultivation in China. Church reports the comj)osition of a considerable number of millets used as food in India, and discusses their food value at some length. The comi)ositiou of the sample is shown in the following table: Table 15. — Composition of millet. to 5ti M -i 2 '3 a r3 . be to . B a 'S o a • 1-1 Panicum sp. : Per ct. Per ct. Per ct. Per ct. Per ct. Per ct. Per ct. P. ct. P.ct. P.ct. P.ct. Original material 11.60 8.56 8.08 0.48 2.64 67.47 0.06 0.00 3.32 1.79 4.56 VV ater-l'ree substaii ce: 9.68 9.14 .54 2.94 76.32 .07 .00 3.75 2.02 5.16 Panicum italicum: a Orisiual material 12. 04 7.40 6.64 .76 3.87 45.73 628.48 1.37 1.11 Water-free substance Panicum miliaceum: c 8.43 7.56 .87 4.40 51.99 632 38 1.54 1.26 Original material 12. 12.6 3.6 (i69.4 1.0 1.4 14.3 4.1 d78.9 1.1 ].6 aTteported by Kellner, Landw. Vers. Stat., 30 (1884), p. 42. 6 Determined by ditlereuce. c Reported by Church, loc. cit., p. 43. d Carbohydrates by difference. For the sake of comparison, two analyses, one of a Japanese- grown sample, the other of a sample grown in India,' are included in the table above. All three analyses confirm the statement that in composition this grain ranks with the more commonly cultivated cereals. It is pos- sible that it might find an important place among our cultivated grains. FRUITS, NUTS, AND FLOW^ERS. Nepheliuni litchi. — This tree furnishes the well known "li chee" or "lai chi" nuts (^;f^), which are so frequently used as tokens of good will by the Chinese laundryman or vegetable peddler about the time of their New Year. The edible portion of the li chee nut is really the fleshy aril which immediately surrounds the smooth brown seed and is in turn surrounded by the thin warty shell of the fruit itself. The nuts may be obtained in the dry form and also preserved with sugar in cans. In the sample analyzed the aril formed 40 per cent of the weight of the dry seed, but these figures can scarcely be considered typical as the fruits were kept for some months before being used. The composition of "li chee" nuts is shown in Table 10, p. 45. As might be expected from their sweet taste, sugar, especially reducing sugar, is their most important constituent. Other nutritive substances are present in small amounts. The tree has been a favorite subject for greenhouse treatment in temperate climates for many years and is also reported to tolerate the * Food Grains of India, pp. 34-59. London, Chapman «fc Hall, 1886. 43 climatic conditions found in southern California in a satisfactory- manner. Nephelium longan. — The fruit of this tree, designated by the char- acters HB^gJ, differs from that of N. litchi in beiug somewhat smaller and smooth, otherwise it closely resembles the "li chee" nut. How- ever, it is considered inferior to the li chee by the Chinese. The sample was kept for some time before analysis, and hence the figures for water content and those based on this determiuation are scarcely typical. The aril formed 40 per cent of the entire seed. Its composition is shown in Table 16, p. 45. In composition it does not differ greatly from the "li chee" save in the relative amounts of reducing and cane sugars present. Zizyphus jujuha. — This is the Indian fig largely cultivated in trop- ical Asia, Africa, and Australia, and is designated by the characters ifiH^- ^^^ dried fruits are used by the Chinese in the same manner as the two mentioned above and are also well liked by Europeans. The European species [Z. vulgaris), is, however, better known and fur- nishes the important constituent of the jujube paste of the confec- tioners' shops. The composition of the fruit of Z. jujuha is shown in Table 10, p. 45. Ganarium album. — The so-called Chinese olive is the fruit of several species of Canarium, C\ album and G.pimela being probably the two species whose fruit is chiefly used. The Chinese olive is a fleshy drupe 3 to 6 centimeters in length, which contains a hard, triangular, sharp- pointed seed. (See PI. II, fig. 6.) Within this are found one or more oily kernels. The fruits, known as "pak lam" (|^^), are found on sale in San Francisco, either green or salted and dried. They are also sold in the form of certain highly flavored preparations which are not generally acceptable to the American palate. The flesh of the fresh, yellowish-green fruit, like that of the true olive, is somewhat acrid and disagreeable, and requires special treatment before it can be made pal- atable. Smith ^ says that the fruits are often preserved in salt, and are also added to wine to moderate or counteract its effect. The sample analyzed by the author (see Table IG, p. 45) represents the fresh pulp of the fruit. The most important constituent is fat, which forms nearly one-fourth of the total nutritive material. The kernel of the seed of one variety of Chinese olive is also sold under the name of "lam yen" (/f^fn). The peculiar form of these seeds is shown in PI. II, fig. 7. They are covered with a thick, reddish skin, but have the consistency and general characteristics of many other oily seeds. Microscoi)ical examination showed the presence of well-formed aleurone grains, the crystalloids of which are remarkably well developed. The analysis of the seeds (reported in Table ]6) shows that in comi^osi- ' Contributions Toward the Materia Medica and Natural History of China, p. 50. Shanghai, 1871. 44 tion they closely resemble ■walnuts and similar nuts. Thefiat present consists of a yellow fluid oil, which absorbs 83.17 per cent of iodin. AVhen fresh, the kernels are as palatable as walnuts and other common nuts. Little is known as to the adaptability of Chinese olive trees to other than tropical climates. It is questionable whether many j)ortions of the United States would furnish the conditions favorable to their growth. Ginlcgo hiloha {SaUsburia adiantifolia). — A detailed account of this tree seems scarcely necessary, since it is well known — by nurserymen, at least — throughout the United States, though its merits as an orna- mental tree are by no means fully appreciated. The trees grow readily and have fruited abundantly as far north as Washington, D. C. The white, thin-shelled nuts (PI. II, fig. 2), known as "pak ko" or "gink ko" (g|g), are eaten by the Chinese, being first roasted or boiled, like chestnuts. They are somewhat acrid in the fresh state, and have a very disagreeable odor. When roasted or boiled, their flavor is peculiar. Tbey are said by Hanbury,^ Smith, and others to assist in digestion and to be eaten after meals for this purpose by the Chinese. The pulp surrounding the seed of the fresh fruit has been examined by Bechanip,- who obtained from it the entire series of homologous fatty acids, from formic acid to caprylic acid. He also noted the occurrence of starch in the endosperm, though a complete analysis of the seed does not seem to have been made. The nuts found in San Francisco were evidently unusually dry. The analysis (Table IG, p. 45) shows that by far their most important constituent is starch. This is somewhat remarkable, as it is the only instance of which record has been found in which starch has been observed in any considerable quantity in the seed of a coniferous plant. Some experiments were conducted by a student in this laboratory to determine whether the seed contained a digestive ferment. Samples of fish flesli were treated under suitable conditions with a decoction of the seeds, but no digestive action was observed. Hemerocallis fulva. — Still another and very unusual vegetable sub- stance largely used as a flavoring ingredient by the Chinese consists of the dried flowers of II. fulva., the day lily of American gardens. This substance is known as "-kam cham t'soi/' or the "gold-needle vege- table," and is designated by the characters '^%\'^. It is reported by Bretschueider ' to be in common use. As noted on page 20, Davy* refers to the use of the flowers of Xi7i»m hulhiferum and H. (jraminca as food by the Chinese. The composition of flowers of H. fulva is shown in Table 16, p. 45. ' Notes on Chinese Materia Medica, Scientific Papers, 1876. ^ Compt. Keud. Aca