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 (Amendmenl IT to H. a. I. Order _7t;> (Effective April 20, 1943) 
 
 United States Department of Agriculture 
 
 AGRICULTURAL RESEARCH ADMINISTRATION 
 BUREAU OF ANIMAL INDUSTRY 
 
 ORDER AMENDING REGULATIONS GOVERNING THE PREPARATION, 
 SALE, BARTER, EXCHANGE, SHIPMENT, AND IMPORTATION OF 
 VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS INTENDED 
 FOR USE IN THE TREATMENT OF DOMESTIC ANIMALS 
 
 Pursuant to the authority conferred upon the Secretary of Agriculture by 
 section 2 of the act of Congress approved February 2. 1903 (32 Stat. 792: 21 
 U. S. C. Ill), and by the act of Congress approved March 4, 1913 (37 Stat. 832-833; 
 21 l". S. C. 151-158), and in order better to effectuate the purposes of these acts, 
 Title «>. Chapter I, Subchapter E. Code of Federal Regulations, [B. A. I. Order 276, 
 August 18, 19221, as amended, is hereby further amended effective April 20, 1943, 
 as follows : 
 
 (1) Section 114.18 [par. 2, Circ. Letter 1796, B. A. I., Sept. 29, 1933 J is amended 
 to read as follows : 
 
 Sec. 114.18. Scrum and virus; release for marketing. When the last full day 
 for concluding a satisfactory test ends before noon the product, after having been 
 labeled as required by part 112. together with the test animals, may be released 
 after the regular forenoon observations have been made. When the last full day 
 of a tentatively satisfactory test does not end before noon, the product, after 
 having been labeled as required by part 112, may be released after the regular 
 forenoon observations have been made: Provided, That, if, as the result of a 
 careful examination of the test animals by a veterinary inspector at the regular 
 forenoon observation time of the next regular workday, the test is declared 
 unsatisfactory, the manufacturer shall immediately recall all of the product in 
 order that it may be placed under Bureau lock. 
 
 (2) Section 118.4 [reg. 18, sec. 1, par. 4, B. A. I. Order 276, amdt. 1, Dec. 26, 
 1922, amdt. 5, Jan. 28, 19291 is amended to read as follows: 
 
 Sec. 118.4. Virus pigs; inoculation requirements. Pigs for the production of 
 inoculating virus shall weigh not less than 40 pounds nor more than 125 pouuds 
 each and shall be inoculated only with highly virulent hog-cholera virus. No 
 hog-cholera virus shall be used for inoculating pigs for the production of inocu- 
 lating virus, hyperimmunizing virus as prescribed in section 118.15, or simul- 
 taneous virus as prescribed in section 118.17 and for inoculating pigs in serum 
 tests as prescribed in section 119.33 unless it has been produced not more than 60 
 days prior to use and since completion has been held only in containers of the 
 boro-silicate type or equal of high resistance and low alkalinity and which are 
 properly marked for identification and guaranteed by the manufacturer to be 
 acceptable to the Bureau and to meet the tests developed for determining these 
 qualities. Other virus may be made suitable for inoculation purposes only by 
 passing it through pigs of the same weight and in the same manner as prescribed 
 in section 121.3. Virus derived from these pigs may be used for hyperimmuniza- 
 tlon if the animals react as prescribed in section 118.5. 
 
 (3) Section 118.7 [reg. 18, sec. 1, par. 7, B. A. I. order 276, Aug. IS. 1922, amdt. 3, 
 Nov. 29. 19241 is amended to read as follows: 
 
 UL8L7. Pigs that become visibly sick within three days after they have been 
 examined for admission to the premises as prescribed by section 117.9 must be 
 rejected and either shall be destroyed or handled as prescribed by section 117.14. 
 Whenever the third day for observation of pigs falls on a Sunday or a holiday 
 such observation shall be made on the fourth day after admission to the premises, 
 or inoculation. 
 
 (4) Section 118.37 [reg. 18, sec. 5, par. 3. B. A. I. order 276, amdt 12, May 24, 
 1935] is amended to read as follows: 
 
 118.37. Simultaneous virus; return date. The expiration or return date 
 placed upon the label <>f each immediate or true container of simultaneous virus 
 shall be one of the following: (a) a date within 90 days after the date of manu- 
 facture: Provided, That the simultaneous virus is stored and marketed in con- 
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UNIVERSITY OF FLORIDA 
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 tamers acceptable to the Bureau; (b) a date within 120 days after the date of 
 manufacture when the product is marketed in containers described in section 
 118.4 and is to be exported to a foreign country and the containers thereof are 
 labeled distinctively. 
 
 (5) Section 119.4 [reg. 19, sec. 2, par. 2] is amended to read as follows: 
 
 Sec. 119.4. Hyperimmune hogs; bleedings and times. Anti-hog-cholera serum 
 shall be derived only from hyperimmune hogs which have been subjected to not 
 more than four successive bleedings after each hyperimmunization. The first 
 bleeding shall take place not earlier than 10 days after hyperimmunization, sub- 
 sequent bleedings shall not take place more frequently than once in 7 days, and 
 the last bleeding shall be made on a date not later than 38 days after hyperim- 
 munization : Provided, Final bleeding may be deferred by the Chief of Bureau 
 on special request. These hogs may be bled as aforesaid on any regular working 
 hour of the day of bleeding. However, none shall be passed for food earlier than 
 10 full days after the time of hyperimmunization. 
 
 (6) Section 119.33 [reg. 19, sec. 4, par. 11] is amended to read as follows: 
 Sec. 119.33. Serum tests; virus required. Simultaneous virus or its equivalent 
 
 of high virulence as described in section 118.4 and in sufficient quantities to meet 
 the needs shall be furnished by licensed establishments for use as the inspector 
 in charge may deem advisable for inoculating pigs in serum tests. 
 
 (7) By amending paragraphs (c) and (d) of section 119.36 [reg. 19, sec. 5, par. 2, 
 rules C and D] to read as follows : 
 
 (c) Conditions under which to be declared "unsatisfactory for potency." A 
 serum test will be declared "unsatisfactory for potency" when at least two of 
 the control pigs react as described in paragraph (a) and the following conditions 
 obtain : 
 
 (1) When one or more of the serum-treated pigs become visibly sick subse- 
 quent to the third day after the time of inoculation and fail to fully recover 
 before the test animals are released by a veterinary inspector as provided in 
 this part. 
 
 (d) Test; conditions under which to be declared' "no test for potency." A 
 serum test will be declared "no test for potency" when any one of the following 
 conditions obtains, but such action will not operate to prevent a retest under the 
 provisions of the regulations in this subchapter : 
 
 (1) When one or more of the serum-treated pigs become visibly sick on or 
 before the third day after the time of inoculation and fail to recover within the 
 test period. 
 
 (2) When two or all of the control pigs become visibly sick on or before the 
 third day after the time of inoculation. 
 
 (3) When two or all of the control pigs do not manifest symptoms of hog 
 cholera as described in paragraph (a). 
 
 (4) When two or all of the control pigs do not show lesions of hog cholera 
 upon post-mortem examination as described in paragraph (a). 
 
 (5) When two or all of the control pigs manifest symptoms of hog cholera 
 within seven days as described in paragraph (a) but do not become sick to the 
 degree described in said paragraph. 
 
 (6) When the serum-treated pigs develop during the test period symptoms 
 of any infectious, contagious, or communicable disease (other than hog cholera) 
 which is not caused by the serum used. 
 
 (7) When a condition obtains in any of the test pigs which is not otherwise 
 covered in this section. 
 
 Done at Washington, D. C, this 15th day of April 1943. Witness my hand 
 and the seal of the Department of Agriculture. 
 
 Claude R. Wickard, 
 Secretary of Agriculture. 
 
 U. S. GOVERNMENT PRINTING OFFICE: 1943