L' Y STATE PLANT BOARD July 1950 E-806 United States Department of Agriculture Agricultural Research Administration Bureau of Entomology and Plant Quarantine EFFECT OF CERTAIN SOLVENTS IN DDT EMULSIONS AND SOLUTIONS ON PLANTS TREATED IN WHITE -FRINGED BEETLE CONTROL By Chas. F. Henderson, Irving Keiser, and Otelia F. Bodenstein, Division of Domestic Plant Quarantines After DDT was shown to be the most effective insecticide against white-fringed beetles ( Graphognathus spp.) (Young 1_, 2), studies were undertaken at Long Beach, Miss., and Atmore, Ala., to determine the best methods for applying this insecticide under field conditions. Both soil and foliage treatments were tested and some of these studies are still in progress. This paper deals with the foliage treatments. Several formulations were tested for applying the DDT spray as a foliage treatment. The insecticide was applied as emulsions and solu- tions to field crops grown in test plots located in two separate environ- ments. Emulsions were also applied to ornamentals in both test-plot and field plantings. Suspensions were not included, since they are less adhesive and therefore not so desirable as foliage applications for control of adult white-fringed beetles. EFFECT ON FIELD CROPS 1945 Studies Tests were initiated in 1945 to study the effect of DDT emulsions and solutions on the foliage and on subsequent crop yield. Applications were made with a concentrated-spray machine (developed by the Bureau) which operated at 3 1/2 miles per hour, covered a swath 25 feet wide, and delivered approximately 6 gallons of spray liquid per acre. The middle rows of each swath received the most drastic treatments, especially when the vegetation was tall, and the observations on foliage injury were made particularly on these rows. _l/ The writers acknowledge the assistance of C. L. Stanford, William Breland, and R. H. Nelson, all of this Bureau; B. P. Livingston, chief, Division of Plant Industry, State of Alabama; Clay Lyle, State , entomologist of Mississippi; and personnel of the Alabama State Prison Farm, Atmore, Ala. Initial boiling irts p' .:.-. ri OS 444 1 504 i 388 1 419 1 192 200 1 253 1 2 - Nine emulsion and two solution formulations were tested 2 fluid ounces of id the IndJ quan*. ■ . rente with water B gallons of spray: S' Sp<-i :fic gra V I tj Velsicol AR-50 0. B PD 544-B .990 Koppers 44-1842 1.010 Mentor 25 . 951 Emulsifiable pine tJii .948 o-Solv A . 795 Xyl< . 865 i No emulsifier was added to the emulsifiable pine oil. Triton X-100 was the emulsifier in all other emulsions except that containing Koppers 44-1842, where it was unsatisfactory. Tween 85 was used as the emulsifier with this Koppers solvent. These quantities were usee water to make 6 gallons of spray for the 1 -pound-per-acre, ar.d twice these quantities in 6 gallons for the 2-pound-per-acre, appkc ations. Fur the solutions 1 pound of DDT was dissolved in enough kerosene to make 10 quarts of solution, or in 1 pint of xylene and enough kerosene nake 10 quarts of solution. These solutions were applied at the rate of 2 1,2 gallons per acre for the 1 -pound-per-acre applications. 1 solutions were also applied at the rate of 1 . 65 pounds of DDT per ac: r these solutions 1.65 pounds of DDT was dissolved in enough krros- nake 10 quarts or in 1 quart of xylene and er.ough kerosene to make 10 quarts. These more concentrated solutions were also applied te of 2 1,2 gallons per acre. Experimental Design. - - One type of ex; "ntai design was U Long Beat h and ai at Atmore. The Long Beach ed of 24 plots 18 by 137 feet, each plot containing the toll i ids Sw< potatoes, peanuts, crowder peas, soybeans, and cotton. The 24 pl< Lded into two tiers of 12 plots each. on. DDT in the differ* Events at the rate of i pound p< r ■ lie othi receiving i)i)T at the rate of 2 pounds | ; id > pounds Ln the .solutions. Tin is one check plot In < A- plots wn p within Is various Crop U ted. A uniform strip I <-ld ted the St i ted s I LOUS : Ml tnulations were spplieoTal the f I pound of Li 1 3- constructed auxiliary-tank assembly which could be quickly drained and flushed with only 1 quart of water (fig. 1). After each plot had been treated, the tank and pipe lines were drained and flushed, before the next spray was put into this auxiliary tank. Cotton, crowder peas, sweetpotatoes, and sorghum were the crops tested in these experiments. Peanut and soybean plots located at Goodway, Ala., 17 miles from the prison farm, had the same experimental design and received identical treatments. Figure 1. --Auxiliary spray tank mounted on concentrated-spray machine used for applying experimental dosages with regular field equipment. The amount of injury caused by the different formulations was determined by observing leaf burn at various intervals after each treat- ment. In order to evaluate the results statistically, the degree of injury was recorded as follows: 0, none; 1, trace; 2, very light; 3, light; 4, medium light; 5, medium; 6, medium heavy; 7, heavy; 8, very heavy; 9, severe; 10, very severe. The degree of injury was not correlated with crop yield. However, a spray causing an injury rating as 4 or higher would probably not be permitted by the farmer for use on his crops. Long Beach Tests . --The Long Beach plots received four spray applications from August through October 1945. The average degree of foliage injury on the different crops 1 week after each treatment is shown in table 1. 4 - r. M X a o rf u a w c o o w e al C o •-H •— t £ a> H Q Q £ o u C rt U 3 cu M rt — < 'o - 2 - 1 s a —' 1 1 Q 3 Hi £ —■ A 1 — ■ a cm ■ 0> X en. *"" ' x u II cm T3 10 * s . o « £ u a. u — -« X ~H a CM o <-• o £ U -* X ■ ••-» cm 9 c X Qu X! 75 1 a- CM a o * « * " xi OT I - ♦-* c 01 ■ — ^ n :*■; x ^ *?* — ■ t - ~. x en in 9 - r- ri r^ r. t T ^r O--NNNnuT(0(fl ^ in ~30 — *- — CMCM o — cm en m ^* co co r- l.~. cc O O O -- — 00 00 0) W is i/l O^NNNMt-ina ID to oooocm— 'TCMrn co to o o n - cm ■** co r- co in in oooroo-Hnn-*' in in Ortr1Hr-in^(Oin o o o — o — n cm 9 i OOO— •*-— 'CMC? OOOOOO — — CM < > "o CO I o (J N £ CM i ^ o a oc ^ . ■ ^r (J t in CM *<3| M * *""* o. 2 - . a — c - o *» -. >, — tn ■«*■ CM CM CN CM 4) c a , CM X n r: x CM CM CM CM CM in -c *J B o u . ttf T w. - c r i 2 ■ ■ g L. 3 It is evident from table 1 that the spray containing Amsco-Solv A was the least injurious, followed by sprays containing xylene and Mentor 25. There was little difference between Koppers 44-1842, Velsicol AR-50, and emulsifiable pine oil. PD 544-B was unsatis- factory, as were all the emulsions and solutions containing kerosene. Where emulsions were applied, the 2 -pound dosage of DDT usually caused about twice as much injury as the 1 -pound dosage. However, the solutions did not cause more damage at the higher concentrations. Since twice as much solvent was used for the 2 -pound dosage of emulsions as for the 1 -pound dosage, but the same amount of solvent was used in both dosages of the kerosene solutions, it is apparent that the greater injury with the 2-pound DDT dosage of emulsions was due to the increased amount of solvent rather than of DDT. Kerosene was the least satisfactory of all the solvents tested. At the higher dosage, this solvent was unsatisfactory in both emulsions and solutions for all crops except sweetpotatoes, and even sweet- potatoes showed a rating of 4 with the emulsion. The xylene-kerosene emulsion was the least injurious of the kerosene formulations, 1 -pound applications giving injury ratings under 4. The other kerosene sprays at the 1 -pound dosage gave ratings of 4 or higher in three of the five crops tested. Sweetpotatoes were the least affected by any of the formulations, and peanuts were second in this respect. The former had an average burn rating of 1.1 and the latter 2.0. The ratings of the other crops fluctuated closely around 3.0. To determine the effect of the various sprays on crop production, the soybeans, crowder peas, and sweetpotatoes were harvested, and yield records obtained. The results are summarized in table 2. It is evident that there was no significant difference in yield between treated and check plots when four applications of 1 or 2 pounds of DDT per acre were made on these crops. Furthermore, no correlation existed between the degree of foliage injury and crop yield on any of the individual plots. Table 2. --Average yields (pounds per plot) of crops from DDT-solvent test plots. Long Beach, Miss., 1945 Crop 1 -pound dosage 2 -pound dosage Treated plots Check plots Treated plots Check plots Soybean hay 32. 7 36. / 31/ 40 7 39. sit Crowder peas 8. 3 6. 8. 4 6. Sweetpotatoes 30. 2 33. 5 38. 2 36. 5 _1/ Considerable injury from other insects. In conjui with the u dj made of th< ) posits (ai mined immedia application) and residues (a. 1 . I appl: e plots treated at the rate of 1 pound of DDT per acre to d - ner or not thi insect; bowed tisJ adr I irious solve tested. wen made on 18 cotton leaves (3 from each of its) collected immediately before and after the second, third, and fourth treatments. are summarized in table 3. 'I Its from Amsco A spra .ved the greate- eness five analyses an ! Wi re second in the sixth. This factor was considered in the final selection of the solven used in sprays against white- fringed beetles, but other factors were of greater importance. Table 3. --DDT deposits and residues (parts per million) on cotton foliage treated with the 1 -pound dosage of DDT in various formulations. Long Beach, Miss. ,1945 Solvent Before treatments •.:••• at ments Emulsions: Amsco-Solv A Emulsifiable pine oil Velsicol AR-50 Koppers 44-1842 Xylene Mentor 25 I'D 544-B Kerosene Xylene -kerosene itions: osene lene kerosene 618 428 796 666 618 349 269 399 399 228 3,437 3 186 2 511 2 2 651 432 2 261 : 061 1 433 1 186 I Atmore-Goodway Tests. - -The plot! five applications at the rate of 1 pound through October 1945. S 'hey v.. ie from the headquai '•' ide on tl t of th< fo. bown in table 4. An Solv A was the n v followed t I emulsif Kei is Peanuts n . -7 rt ■ — i < q >> rt a a> Z § T3 o o a a T3 C 3 tuD o o (h CO o g +J c > •rH W 1— 1 CO 3 s 0) H 3 t>jo o c C cd rt •rH Fh 0) rt CD P. 3 a H Q •r- j Q •iH 1 o CD bB T3 a a w •-H 3 +■> I— 1 o a a 0) &H > : 3 o CO •^ LO a; -* i— I 05 H CMcocor>-cooLnoco o *-i «-» eg cm HCMNNNNnmtD O lO e- t- LO CO CO OOOr-t^HCgCO-^t-l "^ Tt< CO OOOOHOrtNfl m co CO OOOOO'-iOOCNJ m Lfj CO OOOhhhNhN CM "t OOOOO'-iOOt-h 3 6 O CM a) •iH "* o C c <: a CO ^ > £ Q, r-l _. ^H Ul •r-t — t u CD o 0) Q t^. a; -*-• >-> kXW 2 CO X X s 8 - i '): Ti Crowder 18 18.5 Sweetpot 101.4 -.1 Cotton 9.7 There was no significant difference in yield between the d and k plots, and no loss could thus be attributed to the use of DI) and, or the various solvents. Squash plants, affect k< rosene sprays. Of 39 plants trea hese formul ■ ns, the average spread was 19.8 inches, and they averaged 1.6 blossoms and 1. 1 fruits per plant, as compared with a spread of 30 inches, and 4. 2 blossoms and 3. 6 fruits per plant on 48 plants receiving emulsions containing other solvents. Selection of DDT Solvent . --The long Beach and Atmore-Goodw.. test plots were located approximately 150 miles apart in envirorune: with different soil types and climatic conditions. Moreover, experimental designs were used. Nevertheless, there was a po- correlation between the results from the two test areas. In both areas Amsco-Solv A was the most satisfactory solvent, followed I ne at Long Beach and xylene or emulsifiable pine oil at Atmore. Mentor 25 was third in desirability at Long Beach and fourth at Atmor K .pers 44-1842 fourth at Long Beach and fifth at Atmor. Velsicol AR-50 fifth at Long Beach and sixth at Atmore; xylene-kerosene emuls at Long Beachand eighth at Atmore; PD >44-B eigh ong Beach and seventh at Atmore; ana kerosene emulsion, xylene -kerosene sol', and kerosene solution in ninth, tenth, and eleventh places, resr rn both areas. The only major discrepancies in U rrela* results with emulsifiable pine oil h held sixth place at I Beach but tied for second at Atmore. In general, the solvents I ling poinl SS dama^: In both an as e f th< e *. i • I burn on the true leav. r the co.. or sage level. Thes- ments were repea ''-rent I ind pea plants on April 16, 1948, but appli --re n tht early morning of a cool, cloudy day. Obs made 48 rs later showed no damagt her th> or pea c or true folia; of the xylenes tested and at a: :e dosage lev< -..ployed. Si: i.ulsion sprays against adui: irhite-fring beetles are applied at rates of 1,2 or 1 pound of DDT per acre, xylene m either coal-tar or petroleum would b< for se. Amsco A-80, a proprietary solvent caused no damage to the t OS of beans or peas at any of the dosages t- In the 1945 tr a, Beach, Miss., and Atmore, Ala., Amsco-Solv A, another propr tary solvent prepared by the same manufacturer, caused the least amoi. of foliage burn on the several crops tested EFFECT ON ORNAMENTALS Since white-fringed beetle infestations often occur in or adjacent to nur .ige treatments are applied to ornamentals in these art Studies were therefore conducted to determine the effort of DDT -xylene lis ion sprays on this type of vegetation. 1945 Studies During thr summer of 1945 tests were conducted at Per- the ef ' DDT xylene emulsion ed ip >f nur- ha rd 1