UNITED GTATSS DSPARTMENT OF AaRICULTUES 
 
 Bureau of Entomology and Plant Quarantine 
 
 Washington, D.C. 
 
 EelDruary 27, I935 
 
 E. E. P. q,— 369 
 
 PACKING LIA-TERIALS EOR ITUHGERY STOCK; PLAl'TTS, AlID SEEDS 
 (Effective Mar, U, 1935; supersedeo H, B,— I32) 
 
 All packing materialG employed in connection with, importations 
 of nursery stock and other plants and seeds under Quarantine No, 37 
 are subject to approval as to such use by the Bureau of Entomolrgy and 
 Plant Quarantine, 
 
 GENSRAl PACKING MTSRIALS EOR NURSERY STOCK, PLANTS, AND SEEDS 
 
 Such materials as sT)hagnum, ground peat, coconut fiber, osmunda 
 fiber, buckwheat hulls, cereal straw (except rice straw), cereal chaff 
 (except rice chaff), excelsior, shavings, sawdust, and charcoal, are 
 authorized for use with nursery stock, plants, and seeds generally, 
 provided they are free from sand, soil, or earth and have not been 
 previously used as packing or otherwise with living plants • 
 
 Packing materials other than those specifically mentioned may 
 be authorized when it has been determined that their use does not in- 
 volve a risk of introducing Insect pests and plant diseases. 
 
 SOIL AUTHORIZED AS PACKING FOR BULBS, CORIAS, NUTS, ANTD SEEDS 
 
 Regulation 7? Quarantine No. 37 » provides that the requirements 
 as to freedom from sand, soil, or earth shall not apply to sand, soil, 
 or earth used for packing the articles enumerated in items Nos. 1, U, 
 and 5 of regulation 3 v/hen such sand, soil, or earth has been previously 
 sterilized or otherwise safeguarded in accordance with methods pre- 
 scribed by the Bureau of Entomology and Plant Quarantine under the super- 
 vision of an authorized inspector of the country of origin, such steri- 
 lization or safeguarding to be certified to by the duly authorized 
 inspector of such country of origin. This provision is alco extended 
 to bulbs and corras imported under special permit under regulation lU, 
 It should be emphasized that the use of sterilized soil and of naturally 
 sterile soil as described below is not authorized for plants or plant 
 products other than bulbs, conns, nuts, and seeds. 
 
 (1) The prescribed method of sterilizatioja is as follows: 
 
 The sand, soil, or earth must be brought t^ a temperatiire of 
 
UNIVERSITY OF FLORIDA 
 
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 -2- 
 
 100 "^ C. and held at or al)ove that temperature for a period of 1 hour. 
 Any method which will maintain the heat throughout the whole roass at 
 the required temperature for this length of time will "be 5ati3factory 
 to the Bureau. The sterilization must "be performed under the supervision 
 of a duly authorized inspector of the country of origin and must be cer- 
 tified to by such inspector, 
 
 (2) Under the provisions of regulation 7 ^or the use as pack- 
 ing v/ith "bulbs, corms, seeds, and nuts, of soil which has been other- 
 wirse treated the following are authorized: 
 
 • (a) Subsoil from Japan , collected and handled 
 
 under the supervision of the Imperial Plant 
 Quarantine Station at Yokohama, Japan, when 
 certified by the director of that station that 
 the subsoil has been taken from at least 2 feet 
 below the surface, and that it has been sifted, 
 • , dried, and stored so as to prevent contamination by 
 insects and diseases. 
 
 (b) Dune sand from Belgium and the Netherlands , taken 
 from a dej)th of 5 feet or more below -the surface 
 and so certified by an inspector of the phyto- 
 sanitary service of the country concerned. 
 
 (c) Coral sand from Bermuda, uncontaminated by surface 
 soil, and certified as such by the Director of 
 Agriculture of Bermuda. 
 
 Chief, Bureau of Entomology and Plant Quarantine