STATE PLANT BC UNITED STATES DEPARTMENT OF AGRICULTURE B\ireau of Entomology and Plant Quarantine Washington, D. C. 3. E. P. Q. U7U May 7, I93S (Supersedes H. 3. l6l and supplements P. Q. C. A. 309) JAETHOD USED FOR THE DISINFECTION OF IMPORTED BROOMCORN AND BR00MC0RN 3R00MS 3roomcorn and articles made of broomcorn which are required to "be treated, under the provisions of regulation 5 of Quarantine No. kl t will he treated by one of the following methods: I. Vacuum Pamigation: (1) The temperature of the stalks and of the fumigation chamber during the fumigation shall be net less than 60 F. (2) The dosage for the fur.i~aticn shall be 3 pounds of liquid hydrocyanic acid or its equivalent per 1,000 cubic feet of space. (3) The air pressure in the fumigation chamber shall be reduced to the equivalent of 2 inches of mercury (a 28-inch vacuum at sea level), after which the hydro- cyanic acid shall be introduced and the low pressure held for the duration of the fumigation. (k) The e:;posure shall be not less than 3 hours. II. Steam Sterilization: (1) The air pressure in the treating chamber shall be reduced to the equivalent of 5 inches of mercury (a 25-inch vacuum at sea level). (2) Steam shall then be introduced until a positive pressure of 10 pounds is obtained. (3) The exposure to the 10-pound positive pressure of steam shall continue for a period sufficient to assure a constant temperature in all parts of the treating chamber, after which the st-an nay be shut off and the treating chamber exhausted of the un- condensed steam. AVERY S. HCYT, Acting Chief. Bureau of Entomology and Plant Quarantine UNIVERSITY OF FLORIDA