B. E. P. Q. 503 STAAC' r**^ February 28, 19U1 Second Revision TITLE 7--AGRI CULTURE - CHAPTER III— BUREAU OF ENTOMOLOGY AND PLANT QUARANTINE. PART 301— DOMESTIC QUARANTINE NOTICES WHITE-FRINGED BEETLE -'•'•■ [ Pantomoms leucoloma and P. peregrinus ] § 301 . 7 2-5c Administrative instructions modifying the restrictions of the white-fringed beetle quarantine "by authorizing treatment "by methyl bromide solution of "balled nursery stock of specified thickness Introductory note . The instructions in circular B, E. P. Q. 503 authorizing the treatment of nursery stock by a solution of methyl bro- mide, as last revised (March 15, 19^0), provided for specified treatments of larvae of each of the species of white-fringed beetle, Pantomorus leu - coloma Boh., the more common species, and P. peregrinus Buch. , the new species. As a result of further investigations, the instructions are re- vised to provide for a modified treatment applying to both species and to all regulated areas other than in New Orleans and vicinity. The treat- ment authorized in the last revision of the circular is continued for the New Orleans area for the reason that the consistency of the soil in that area is such that the treatment can be used effectively only on soil balls not greater. than 7 inches in diameter. In other areas, effective results are obtained on soil balls up to 8 inches in diameter. The specifica- tions for treatment in the New Orleans area are brought forward in the instructions which follow, (a) Treatment authoriz ed. Pursuant to the authority conferred upon the Chief of the Bureau of Entomology and Plant Quarantine by sub- jection (a) of §301.72-?, Chapter III, Title 7, Code of Federal Regula- tions (Regulation 5 of Notice of Quarantine No, 72 on account of the white'-f ringed beetle), the following method of treatment for balled plants' is hereby authorized when carried out under the supervision of an author- ■ ized inspector of the United States Department of Agriculture. (*b) Type of material authorized . (l) In Orleans Parish, includ - ing the city of New Orleans, and Saint Bernard Parish, La . The treatment shall be applied only to plants in soil balls not greater then 7 inches in diameter nor greater than 7 inches in thickness when not spherical. ( 2 ) In al l regulated area other than the parishes of Orleans and Saint Bernard, La . The treatment shall be applied only to plants in sail balls not greater then 8 inches in diameter nor greater than 8 inches in thickness when not spherical. (c) Treatment metho d, (l) In all regulated areas . UNIVERSITY OF FLORIDA lllllllllllllllVMlllllllllllllllllVlllll 3 1262 09312 1639 (i) The soil "balls around the roots of plants must be buried in sand and plunged in boxes or trays approximately 1 foot deep, which are watertight. (ii) A 2-inch space filled with sand shall be provided between the soil balls, also above and beneath them. (iii) Such soil balls shall be treated with a solution of methyl bromide and alcohol at a concentration of 0.3 percent methyl bromide and 0.6 percent denatured ethvl alcohol by volume in water. The solution is to be prepared by first mixing the methyl bromide and alcohol together and then adding this mixture to the water and mixing thoroughly. (iv) The aqueous solution of methyl bromide and alcohol shall then be ap-olied evenly over the surface of the sand around the plants at the rate of Uo gallons per 100 square feet of surface area by means of a sprinkling can or sprayer. (2) Exposure and temperature . (i) In Orleans Parish including the city of Hew Orleans, and Saint Bernard Parish, L a. After the required dosage has been applied, the' soil balls shall remain embedded in the sand for a period of 8 hours. The temperature of the soil balls during the treatment shall not be lower than 65° F« (ii ) In all regulated area other than Orleans Parish and Saint Bernard Parish, La . After the required dosage has been applied, the soil balls shall remain embedded in the sand for a period of 8 hours. The tem- perature of the soil balls during the treatment shall not be lower than 55° F. (d) Disclaimer . There has been opportunity to test this treatment on only relatively few varieties of plajits, and it is understood that no liability shall attach either to the United States Department of Agricul- ture or its employees in the event of injury to either plants or operator s. (e) Caution . Methyl bromide is a gas at ordinary temperatures. It is colorless and practically odorless, and in preparing the solution the operator should wear an a/oproved gas mask. This revision supersedes all previous issues of circular B. E. P. Q. 503. AVERY S. HOYT, Acting Chief, Bureau of Entomology and Plant Quarantine