:;Xi''' -■ 706 V. Wm^f^B &^-m B Bm^B- THE UNIVERSITY OF ILLINOIS LIBRARY NATURAL HISTOEY SURVEY 5705 ILL V. 2.cop.4 .?; B B E B ilk £ e B E E E E E t b t ILLINOIS BIOLOGICAL MONOGRAPHS Vol. II April, 1916 No. 4 Editorial Committee Stephen Alfred For^bes William Trelease Henry Baldwin Ward Published under the Auspices of the Graduate School by THE University of Illinois Copyright, 191 5 By the University of Illinois Distributed June 30, 1916 THE GENUS MELIOLA IN PORTO RICO Including Descriptions of Sixty-two New Species and Varieties and a Synopsis of all Known Porto Rican Forms. WITH FIVE PLATES BY FRANK LINCOLN STEVENS Contribution from the Botanical Laboratories of the University of Illinois TABLE OF CONTENTS Introduction Page 7 Key to Sections _ 10 Section A 10 Section B 12 Section C 20 Section D 32 Section E 34 Section F „ 52 Section G 54 Section H 57 Host List 66 Explanation of Plates _ 75 Alphabetical list of Species 85 475] MELIOLA IN PORTO RICO — STEVENS THE GENUS MELIOLA IN PORTO RICO The genus Meliola while not the largest or the most important genus of parasitic fungi in Porto Rico possesses features of special interest. As at present understood from the monograph of Gaillard, it constitutes a well defined, clearly marked genus with a fairly large number of species. In the Sylloge Fungorum of Saccardo 234 species and varieties are included; 63 additional species have since been described, making 297 species and varieties recognized prior to the present paper. The fungi are especially beautiful in contour, superficial with small penetrating haustoria. Usually their effect upon the host is slight though in some instances well marked pathological effects are discernible. They are among the most common genera of leaf inhabiting fungi in Porto Rico, and may be found in all parts of the island in great abundance, usually, though not always, causing conspicuous black blotches upon one or both surfaces of the leaf, more rarely upon stems. The present study is based almost entirely upon collections made by the writer during the years 1912, 1913, and 1915. Cognizance is ta- ken also of the collections of Heller reported by Earle. Nearly every species noted by earlier writers is included in my own collections and is usually reported upon several hosts and from numerous localities. A narrative of the two collecting trips which furnished those speci- mens is given in the Journal of the New York Botanical Garden, June 1916. A reference to the time involved has been made by Arthur.^ The specimens, types, cotypes, etc. of this collection have been depos- ited as indicated by Miss E. Young.- Other articles based on these col- lections have been published by P. Garman,' E. Young,* J. C. Arthur.' ^Mycologia 7:168, 1915, and 8:20, 1916. 2Mycologia 7:143, 1915. sMycologia 7 -.333, 1915. ■*Mycologia 8:42, 1916. 'Mycologia 7:168, 227, 315, 1915; 8:16, 1916. 8 ILLINOIS BIOLOGICAL MONOGRAPHS [476 The following summary will serve to give a general idea of the scope of the present report : Total number of collections of Meliola 729 Number of species of Meliola 95 " varieties of Meliola 6 ' ' new species of Meliola 56 " new varieties of Meliola.. _ 6 ** species and varieties of Meliola new to Porto Rico 83 " species and varieties of Meliola previously reported from Porto Rico 20 " hosts 171 " " previously reported _ 25 " " new to Porto Rico for Meliola 146 " host families 53 " " '* previously reported 18 " " " new to Porto Rico for Meliola 35 In grouping the genus into sections and in keying the species I have in the main followed the plan adopted by Gaillard, laying principal emphasis upon the setae, perithecial and mycelial, next upon the char- acter of the setal tips, and basing final specific distinction upon more minute characters of mycelium, capitate hyphopodia, spore, etc. It is to be noted that in Porto Rico occur none of the species with clavate, long, 8-spored asci. All are of the short-ascus form with two to four spores per ascus. In certain species there is constancy as regards each character, in other species there is large variation. Young colonies may be devoid of mycelial setae though they may be abundant in old colonies (M. earlii). Certain species sometimes though not always present perithe- cial setae (M. solani). Several species show large variation as to setal tips, the tips being sometimes simple and acute, sometimes slightly forked, (M. mangiferae, M. ipamoeae), even much forked, and all aris- ing from the same mycelium; or the forking may show a great range in character and complexity (M. furcata). In other species the setal tips may show great constancy, e.g., M. cucurhitacearum. Taken all in all the problem of specific limitation is much the same as is met in the Erysiphaeeae, with very similar characters to use. The problem of classification is rendered especially difficult by the fact that the Meliolas are very often overgrown by parasites which con- fuse the picture and also often prevent the formation of perithecia, and, I believe, strongly influence the character of the mycelium and hypho- podia, i.e., the mycelium and hyphopodia are often made more crooked and more angular, or to appear so, by the parasite that is growing upon them. I believe also that the character of the mycelium is strongly 477] MELIOLA IN PORTO RICO — STEVENS 9 influenced by the nature of the leaf upon which it grows, that it will be more nearly straight on a straight-veined, smooth leaf than upon a crooked-veined, hairy one. The question of limits of variation in mycelium, hyphopodia and setae is a difficult one and finally will be solved only by inoculation experiments and observations upon live material. I have left out of all consideration the so-called conidial stage, Hel- minthosporium, Podosporium, etc., because these structures appear use- less in taxonomy and, moreover, because I am far from convinced that they really are conidial stages of the Meliolas. The mucronate hyphopodia are curious structures but they are so variable on the same mycelium that they possess little value in classification. The question of biologic specialization has been but little studied in this genus. My field observation, however, strongly supports the idea that there is large specialization. For example, Cissus vines heavily coated with M. merrillii are found with their branches interlacing with plants susceptible to Meliola but the Cissus Meliola does not invade the other plants. The Meliolas on Dieffenbachia and Philodendron, mem- bers of the same family, to the naked eye appear much alike and under the microscope are kin, yet there is no field evidence that one goes to the other's host. In some cases forms morphologically indistinguishable are found upon large numbers of species or genera in the same family (notably M. hicornis on the Leguminosae), whether these forms are inter- inoculable or not is not known. It is possible that were type specimens of all the old species at hand for comparison some of the new species might be found to agree with them, but from the descriptions and figures available such does not seem to be the case. It is more probable that some of the collections which I have referred to old species, relying upon printed descriptions and without comparison with types, in reality may be new species. It seems best for present purposes, however, to avoid undue multiplication of species and I believe that my error lies more largely in conservatism than in the creation of new species. It is extremely difficult to interpret many of the descriptions that have been written, some are too brief and leave out many essentials, others describe setae but do not state whether they are perithecial or mycelial and the supposition is strong that there have been many cases where the colony character has been taken from a colony parasitized by some other fungus, e.g., many of the Meliolas described as having a crustose colony are probably Meliolas parasitized by a Microthyriaceous fungus. The final word as to the specific relation cannot be said but with the large number of collections at my disposal, on many hosts, and the 10 ILLINOIS BIOLOGICAL MONOGRAPHS [478 opportunity thus afforded of making close comparisons between these forms, I feel that I have at least approximated the truth. Especially worthy of note is the occurrence in Porto Rico of sev- eral tropical species on closely related hosts, originally described from the other side of the world, notably M. merrUUi, on Sicyos from the Philippines, M. clavnlata on Ipomoea and M. cyperi on Sedges from Africa. It should be remembered that Meliola is preeminently a tropical genus, almost exclusively so, the occurrence therefore of these species in Porto Rico, Africa and the Philippines with no present tropical land connection between these countries implies, either that the Meliolas have in the past been of different climatic range or that they are the residual flora of previously connected tropical lands. I wish to acknowledge the very great assistance that I have received from Doctor N. L. Britton and Mr. Percy Wilson in the determination of Phanerogamic hosts, from Miss Slosson, the Pteridophytes, and from Mrs. Agnes Chase the Grasses. Meliola, Key to Sections Spores 3-septate, mycelial setae none section A, p. 10 Spores 4-septate Mycelial setae none Perithecium with no setae or appendages Perithecium with setae or with appendages Mycelial setae present Perithecium with setae or appendages Perithecium with no setae or appendages Mycelial setae simple, straight Mycelial setae simple, uncinate Mycelial setae usually simple, some- times forked Mycelial setae forked Section A Spores 3-septate, mycelial setae none The members of this section are clearly marked by the 3-septate spores, a character that seems to be invariable, and the three species found in Porto Rico are clearly limited as is shown in the following key. Key to Species of Section A Perithecium with larvaeform appendages M. puiggarii No. 1 Perithecium with no larvaeform appendages Perithecial setae absent M. manca No. 2 Perithecial setae present, M. guignardi No. 3 section B, P- 12 section C, P- 20 section D, P- 32 section E, p. 34 section F, P- 52 section G, P- 54 section H, P- 57 479] MELIOLA IN PORTO RICO — STEVENS 11 There is quite a striking similarity between the hyphopodia upon the three species with 3-septate spores. They are of the same general size and contour, large and irregular. 1. M. PuiGGARH Speg. Fungi Puiggariani. No. 228. On Rubus sp. Fig. 1.^ El Alto de la Bandera, July 15, '15, 8650, July 14, '15, 8270. Maricao, July 20, '15, 8892. No other species has been reported upon the Rosaceae. The form agrees well with the descriptions and with a type specimen, No. 2722, received from Dr. Spegazzini, though the perithecial appendages are nearly twice as long as stated. See also under M. manca. 2. Meliola manca Ell. and Mart. Amer. Nat. 17 : 1284, 1883, and Jour. Myc. 1 : 148, 1885. On Myrica cerifera L. Fig. 2. Manati, Nov. 23, '13, 5289, Nov. 25, '13, 5250. A collection by Heller near Catano, Jan. 17, '03. In **Le Genre Meliola", Gaillard unites under M. manca the three species M. manca, M. sanguinea, and M. puiggarii, giving a new de- scription for M. manca. This new description mentions larvaeform, perithecial appendages. The original description of M. manca by Ellis and Martin mentions no such appendages, but does specifically state that there are no perithecial appendages. My own material of two collections is ample. I have studied it carefully, also a specimen of the Heller collection and a specimen collected by Martin and distributed by Ellis as N.A.F. No. 1292, all of these upon the same host. These speci- mens all agree perfectly and agree with the original description. They do not have larvaeform perithecial appendages and therefore do not conform with the description as given by Gaillard. The specimens of Meliola which I have found on Rubus agree well with the description of M. puiggarii. They have abundant larvaeform appendages and cannot be placed under M. manca. Moreover, both the general characters of the mycelium and of the capitate hyphopodia separate the forms on Rubus from those of Myrcia, the hyphopodia on Rubus being much larger and more irregular in shape. I am forced therefore, to regard the description given by Gaillard for M. manca as ^Figures accompanying this article are noted in connection with the host from which they were made. 12 ILLINOIS BIOLOGICAL MONOGRAPHS [480 erroneous and that of Ellis and Martin as correct and to recognize M. puiggarii on Rubus, as an entirely different species. In the head cell of each capitate hyphopodium there is a small, clear spot, about 3/i in diameter, which appears to be a hole in the lower side of the hj^phopodium, and if so probably indicates the breaking off of a haustorium. Similar marks are found upon the capitate hyphopo- dia in many species but they are not usually so conspicuous as in this species. 3. M. GUIGNABDI Gaill. Bull. Soc. Myc. de France 8: 176, 1892. On Turpinia panniculata Vent. Fig. 3. Maricao, July 19, '15, 8922, Sept. 20, '13, 3685. This differs somewhat from the type as described by Gaillard in its smaller perithecia, about ZOOfi, shorter perithecial setae, about 100- 150/*, and smaller ascospores, 54 x 15fi. It is of. special interest since it is the only species known which has a 3-septate spore, without mycelial setae, and with perithecial setae. The hyphopodia are verj^ characteristic and agree well with the figures of Gaillard. The type of the species was from Ecuador on host unknown. SECTION B Spores 4-septate, no setae or appendages This section corresponds to the Section I C. a. of Gaillard without the forms with perithecial appendages. As represented in Porto Rico it presents many puzzling aspects, occurring on a large number of hosts of many families and presenting variations which though easy to recognize are difficult to define with accuracy. The spores in all are quite uniform and neither in size, shape nor constriction lend themselves to description. There are no perithecial or mycelial setae. Therefore the perithecium, the mycelium and hyphopodia must furnish the dis- tinctions. Moreover, such variations as do occur in the perithecium are minor and difficult to state definitely. For this reason many students have referred such forms on numerous hosts to M. glabra. A study of the Porto Rican material both in the field and under the microscope convinces me that many of these forms which may agree in spores and perithecia and which show variation in general habit in mycelium and hyphopodia are really distinct species. Plants heavily infected with one of these forms of Meliola may be found growing among plants sus- ceptible to another form, but which are entirely devoid of Meliola. In the treatment of the Porto Rican species I have removed from M. glabra all which it seems very evident are not co-specific with the 481] MELIOLA IN PORTO RICO — STEVENS 13 specimen No. 3849 of Rabenhorst's Fungi Europaei. I have described some Meliolas as varieties though I am inclined to believe that in several instances they will eventually prove to be really as distinct specifically as many other forms that are recognized as species. Key to Species of Section B Mycelium very dense, close woven Spore cells about equal in size Head cell oval or slightly angular M. glabra No. 4 Head cell angular to lobed M. sepulta No. 5 Spore cells unequal M, irregularis No. 6 Mycelium not close woven Capitate hyphopodia mostly opposite M. solani No. 7 Capitate hyphopodia not opposite Mycelium more or less crooked Mycelium wavy Capitate hyphopodia oval M. hyptidicola No. 8 Capitate hyphopodia globular Perithecia very rough M. cyclopoda No. 9 Perithecia not very rough M. aibonitensis No. 10 Mycelium very crooked Capitate hyphopodia angular M. perseae No. 11 Mycelium usually straight or nearly so Capitate hyphopodia entire Oval, small, 17 x Ifi M. lagunculariae No. 12 Elliptical, large, 21 x 10)U M. longipoda No. 13 Pyriform M. melastomacearum No. 14 Capitate hyphopodia entire or lobed M. glabroides No. 15 Capitate hyphopodia lobed M. triloba No. 16 4. Meliola glabra Berk, and Curt. Cuban Fungi N. 883 (pro parte). Gaillard, Le Genre Meliola : 59 : 1892. ~ On Hypelate trifoliata Sw. Mona Island, collected by Britton, Cowles and Hess, Feb. 1914. Two other specimens were also sent to me by Dr. Britton from the Bronx Herbarium, one from the Bahamas, No. 8246, one from Cuba, No. 2171, collected in 1860-1864. On Drypetes sps. Rio Tanama, July 6, '15, 7885. This specimen shows an unusually rough, irregular mycelium and capitate hyphopodia. 14 ILLINOIS BIOLOGICAL MONOGRAPHS [482 If we accept for M. glabra the description given by Gaillard and as belonging to it the specimen distributed by Rabenhorst and Winter as No. 3849, Fungi Europaei (See Fig. 4) on Barbacenia purpurea we must think of this as a form with a dense, closely matted mycelium with mycelial threads coursing more or less parallel. This is typically the condition of the Meliola on Hypelate. 4a. Meliola glabra var. psychotriae var. nov. There is a remarkable variation here in that many of the colonies are very small, of rapidly branching, closely matted mycelium, while others are of looser structure and larger in area. The small, closely matted colonies approach quite closely to the type while the loose colonies deviate from it widely. On Palicourea domingensis (Jacq.) DC. Florida Adentro, July 1, '15, 7649 (type). On Psychotria hertiana P. El Alto de la Bandera, July 15, '15, 8673, 8654, 8278, 8710, 8566, 8646. El Gigante, July 17, '15, 8528. On Psychotria puhescens Sw. Arecibo - Lares Road, June 21, '15, 7281. Vega Baja, July 2, '15, 7732, July 2, '15, 7741. Dos Bocas, below Utuado, July 8, '15, 8032. On Psychotria grandis Sw. Mayaguez, June 25, '15, 7487. On Psychotria sp. Quebradillas, Nov. 22, '13, 5032. Vega Baja, Mch. 1, '13, 516. On Palicourea 1 Mayaguez, May 1, '13, 1070 b. Jan. 30, '13, 316. Vega Baja, Feb. 20, '13, 468. Dos Bocas, below Utuado, Dec. 30, '13, 6650. El Gigante, Dec. 15, '13, 5944. Ponce, Nov. 8, '13, 4367. Maricao, July 20, '15, 8875. On Coccocypselum repens Sw. Maricao, July 20, '15, 8961. 5. Meliola sepulta Pat. in herb. On Avicennia nitida Jacq. Fig. 5. Two specimens were collected by Heller, Nos. 390, Jan. 31, '89, 6416, Jan. 17, 1903 ; both of these in the Bronx Garden bear the above name, but description has not yet been published. The following de- scription is made from a fragment of specimen No. 390 which becomes the type: Colonies circular, 1-7 mm. in diam., black, dense, mostly hypophyl- lous, mycelium closely branched, producing a closely woven mat. Capi- 483] MELIOLA IN PORTO RICO — STEVENS IS tate hyphopodia 24 x 14/*, opposite or alternate, head cell angular or often distinctly lobed, stalk cell 10/* long. Mycelial and perithecial setae none. Perithecia abundant in older portions of the colony, ITOju, in diam., slightly rough. Asci evanescent, short, thick, 2-4 spored. Spores,. 52 X 20/A, strongly constricted, ends obtuse. 6. Meliola irregularis sp. nov. Colonies amphigenous, irregular, 1-3 mm. in diam., black. My- celium forming a very close network of threads which branch irregu- larly, Tfx in diameter. Hyphae often so close as to lie in contact form- ing a mat. Capitate hyphopodia alternate or irregular, crowded, head cell pyri- form, somewhat irregular, 17 x 10/x, the basal cell 3-7/* long, Mucronate- hyphopodia alternate or opposite, bottle-shaped, narrow, 20/a long. My- celial setae none. Perithecial setae none. Perithecia 200/a, rough with conic protuberances about 17/x high.. Asci 2-spored, soon evanescent; ascospores 4-septate, brown, constricted^ obtuse, 41 X 14/A, often irregular in shape, one end pointed, one or more cells larger than the others, or with septa set obliquely, occasionally with an aberrant number of septa. On Hygrophila hrasiliensis, (Spreng.) Lind. Fig. 6. Rio Piedras, Aug. 11, '15, 9283 (type). This species in character of perithecium resembles M. glabra but in spores and mycelium it is quite different. 7. Meliola solani sp. nov. Colonies amphigenous, irregularly circular, 2-4 mm. in diam. My- celium black, forming a close network, 7/* in diameter, branches irregular. Capitate hypophodia small, mostly opposite, not crowded, 2 per cell, cells about 17/* long, head cell pyriform or globular, 10 x 8/*, the basal cell short. Mucronate hyphopodia few, alternate or oppo- site, bottle-shaped, 14 x 7/*. Mycelial setae none. Perithecial setae few or wanting, straight or slightly curved, 125 x 7/i, tip obtuse, dark. Perithecia 150-180/*. Asci soon evanescent; ascospores 4-septate^ brown, very slightly constricted, obtuse, 37 x 14/t. On Solanum jamaicense Mill. Fig. 7. Monte de Oro, Dec. 3, 13, 5750 (type). The chief, distinguishing character is the small, nearly globular capitate hyphopodia which are usually opposite. 16 ILLINOIS BIOLOGICAL MONOGRAPHS [484 8. IVIeliola HYPTmicoLA sp. nov. Colonies amphigenous, chiefly h3T)ophyllous, irregular, diffuse. Hycelium black, forming a loose network, 7/t in diameter, branches irregular. Capitate hypophodia small, alternate or irregular, not crowded, 1 per cell, cells about 20/* long, head cell ovoid, 14-17 x 9/x, the basal cell 3-6/1 long. ^lucronate hyphopodia few, bottle-shaped. Perithecial setae none. Mycelial setae none. Perithecia lOO-140/i, slightly rough. Asci soon evanescent; asco- spores 4-septate, brown, very slightly constricted, obtuse, 31-34 x 10-13/*. On Eyptis lantanifolia Poit. Las Marias, July 10, '15, 8130 (type). On Eyptis capitate Jacq. Fig. 8. El Gigante, July 16, '15, 8526. On Eyptis pectinata (L.) Poit. Dos Bocas, below Utuado, July 7, '15, 7981. Maricao, July 19, '15, S791. On Eyptis sps. Monte de Oro, Dec. 13, '13, 5760. 9. Meliola cyclopoda sp. nov. Colonies epiphyllous, circular, 1-5 mm. in diam. Mycelium black, forming a loose network, 7/t in diameter, branches mostly opposite. Capitate hj-phopodia small, alternate, not crowded, 1 per cell, cells 20 to 35/1 long, head cell nearly globular, 14 x 14/i, the basal cell 3-7/4 long. Mucronate hyphopodia opposite, bottle-shaped, 10-18 x 6/i. Peri- thecial setae none. Mycelial setae none. Perithecia 135-170/1 in diam., rough with conic protuberances. Asci soon evanescent; ascospores 4-septate, brown, slightly constricted, ob- tuse, 38 X 14/1. On Pseudelephantopus spicatus Juss. Fig. 9. Vega Baja, July 2, '15, 7733 i long, 10-14/i thick. Tip obtuse. Perithecia 250)u., slightly rough with rounded protuberances. Asci 78 X 38/i, soon evanescent ; ascospores 4-septate, pale brown, slightly con- stricted, obtuse, 45-52 x 20-22;i. On Amyris elemifera L. Fig. 35. Mona Island, Dec. 20-21, 13, 6158 (type), 6150, 6146. Guayanilla, July 14, '15, 8547. The colonies of this species are characteristic and the capitate hy- phopodia distinctive. 48. Meliola thouiniae Earle Bull. N. Y. Bot. Gard. 3 : 308, 1905. On Allophylus crassinervis Rad. Quebradillas, Aug. 5, 15, 9003. On Thouinia striata Rad. Fig. 36. Vega Baja, July 2, 15, 7756. Rio Arecibo, K. 64.7, July 8, 15, 7773. The type was collected on the same host by Heller near Bayamon, Jan. 21, 1903, No. 6435. The cylindrical, capitate hypophodia, so crowded as to touch each other, give a unique appearance to the colonies on Allophyllus. Study of the type specimen and my own specimens on Thouinia shows varia- tion in the crowding of the hyphopodia. In some parts of the colony they are densely crowded and opposite ; at the edge of the same colony they may be strictly alternate and sparse. On Winterana canella L. Guayanilla, July 14, 15, 8548, July 26, 15, 9075. Mona Island, Dec. 20, 13, 6154. Ponce, Aug. 7, 15, 9189. On Krugiodendron ferreum (Vahl) Urb, Guayanilla, July 14, 15, 8594. Rio Tanama, near Arecibo, July 6, 15, 7895. Quebradillas, Aug. 10, 15, 9247, June 20, 15, 9266. Coamo, Feb. 6, 13, 814, Apr. 6, 13, 831. 49. Meliola didymopanicis P. Henn. Hedw. 34: 106, 1895. On Dendropanax arhoreum (L.) Dec. & PI. Rio Arecibo, K. 64.7, July 8, 15, 7775, Mayaguez Mesa, June 25, 15, 7440. Florida Adentro, July 1, 15, 7647. On Dendropanax laurifolium (E, March.) R. C. Schneider. El Alto de la Bandera, July 14, 15, 8265. 40 ILLINOIS BIOLOGICAL MONOGRAPHS [508 The species was described from Brazilian specimens on Didymopa- nax sps. My material does not agree exactly with the somewhat meagre description, but does agree fully with a co-type specimen (Glazion, Minas Geraes, Brazil 1893) kindly furnished by Dr. H. Sydow. No perithecial setae are evident, though the mycelial setae are often clus- tered around the base of the perithecium, which may account for this character in the description. A striking character in my specimens is the capitate hyphopodia, which are usually opposite, with a regular, oval head cell. The chief distinction between -this species and M. araliae seems to be in the length of the setae. It is possible that M. didymo- panicis should be reported under that name. M. araliae was collected in Porto Rico, by Ule, No. 242 in 1884 on "Aralia arborea". 50. Meliola amomicola sp. nov. Colonies mostly hypophyllous, black, circular to irregular, diffuse, 5-15 mm. in diam. Mycelium rather closely woven, nearly straight, black to pale straw color, branches mostly opposite, 6/x thick. Capitate hyphopodia mostly opposite, two per cell, cells about 24/x long. Head cell cylindrical, straight or crooked, little or no thicker than the stalk cell, stalk cell 2-4/u, long. Mucronate hyphopodia scattered, bottle-shaped, 24 x 6/x. Perithecial setae none. Mycelial setae sparse, scattered, long, 900-1000/u., lOytt thick at base, black throughout, acute. Perithecia scattered, 180/u, in diam. Spores 15 x 11 fi, strongly con- stricted, obtuse. On Amomis caryophyllata (Jacq.) Krug. and Urb. Fig. 37. Mayaguez Mesa, June 15, '15, 7054 (type). Mayaguez, June 25, '15, 7483. 51. Meliola myrsinacearum sp. nov. Colonies amphigenous, more abundant above, irregular, circular, 1-10 mm. in diam., confluent. Mycelium black, forming a close network, 10/x in diam., branches mostly opposite. Capitate hyphopodia alternate, unilateral or irregular, not crowded, 1 per cell, cells about 34/Lt long, head cell nearly cylindrical or slightly pyriform, 20 x 14/i,, the basal cell 2-4yx long. Mucronate hyphopodia few and scattered, alternate or opposite, bottle-shaped, 24 x lO/i. Peri- thecial setae none. Mycelial setae few or absent in young colonies, abun- dant in old colonies, straight or slightly curved, 625-}- x lO/i, dark throughout, tip obtuse. Perithecia 200ju,, somewhat rough. Asci soon evanescent; ascospores 4-septate, brown, slightly constricted, obtuse, 51 x 17-20/x. On Ardisia guadalupensis Due. Mayaguez Mesa, June 29, '15, 7576 (type), 7057. 509] MELIOLA IN PORTO RICO — STEVENS 41 On Myrsinaceae indet, Maricao, July 19, '15, 8905, Oct. 20, '13, 3681. This species is closely related to M. amphitricha but distinguished from it by the shape of the capitate hyphopodia. 52. Meliola pilocarpi sp. nov. Colonies hypophyllous, irregular, 4-15 mm. in diam., black. My- celium forming a loose network of threads, branches opposite or alter- nate, 8/t in diameter. Capitate hyphopodia alternate, rarely opposite, not crowded, one per cell, cells, 20-30)u, long, head cell cylindrical or slightly thickened, 20 X lO/Lt, the basal cell short. Mucronate hyphopodia mostly opposite, bottle-shaped, the neck often curved, 20 x 7/x. Perithecial setae none. Mycelial setae few, scattered, straight, black, 1100/i, long, 10/* thick at base, tip acute. Perithecia 190/a in diam. Asci soon evanescent; ascospores 4-sep- tate, brown, strongly constricted, obtuse, 51-54 x 17-20/t. On Pilocarpus racemosus Vahl. Mayaguez, June 13, '15, 7080 (type). 53. Meliola stenotaphri sp. nov. Mycelium crooked or in zig-zag bends about equal in number to the hyphopodia. Capitate hyphopodia alternate, or for considerable distances uni- lateral, with distinct stalk of much smaller diameter than the head. Stalk cell 7fi wide, 5-9/a long; head cell spherical or oval, about 20 x 14/li. Mucronate hyphopodia scarce, opposite or alternate, ampulliform, 7 X 14yu,. Mycelial setae dark, opaque, about 275/* long, 7/a thick at base, simple and acute or very rarely bifid. Perithecial setae none. Perithecia small, about lOO/i, surface rough with rounded protuber- ances. Spores oblong, 4-septate, obtuse, slightly constricted, 30-44 x 10-14/*. On Stenotaphrum secundatum (Walt.) Ktze. Fig. 38. Manati, Nov. 5, '13, 4304 (type). Rio Tanama, near Arecibo, July 7, '15, 7940 July 7, '15, 7852. Dos Bocas, below Utuado, July 8, '15, 8023. Arecibo, K. 64.7, July 8, '15, 7810. The mycelium is distinctive, rather closely woven and with numer- ous crooks or zig-zags. The long stalks and rounded heads of the capi- tate hyphopodia are also sufficient to separate this from other forms. 54. Meliola capsicola sp. nov. Colonies amphigenous, foliicolous and caulicolous, circular, 1-3 mm. 42 ILLINOIS BIOLOGICAL MONOGRAPHS [510 in diam., black. Mycelium forming a very close network, Ip. in diame- ter, branches irregular, crooked. Capitate hyphopodia alternate, not crowded, 1 per cell, cells about 20 to 30/i long, head cell very irregular in shape and size, the basal cell often long. Mucronate hyphopodia few and scattered, bottle-shaped, irregular, 20 x 7/i. Perithecial setae none. Mycelial setae abundant, straight or slightly curved, 265 x 10/*, dark throughout, tip acute. Perithecia 170/*, slightly rough. Asci soon evanescent; ascospores 4-septate, brown, slightly constricted, obtuse, 41 x 14/i. On Capsicum baccatum L. Fig. 39. Manati, July 2, '15, 7698. Dos Bocas, below Utuado, July 8, '15, 8019 (type). On account of the great variation in the hyphopodia, both in shape and size, the form is noteworthy. 55. ^Ieliola paucipes sp. nov. Colonies amphigenous, mainly below, irregular, scattered, diffuse, 5-10 mm. in diam. Mycelium forming a verj' loose network of threads, branching irregularly, 7/i in diam. Capitate hyphopodia alternate or irregular, distant, 70-100/* apart, ovoid or pyriform, often angular, head cell 17 x 14/*, the basal cell about 4/* long. MucroHate hj-phopodia mostly alternate, bottle-shaped. My- celial setae none. Perithecial setae scattered, very long, 700 x lO/i, straight, simple, tip obtuse. Perithecia 160/*, slightly rough, ostiole none. Asci evanescent ; asco- spores 4-septate, brown, very slightly constricted, obtuse, 37 x 14/*. On Piper hlaUarum, Spreng. Fig. 40. Mayaguez Mesa, June 25, '15, 7463 (type). This species, the only collection upon the host, is distinguished from others by its very long mycelial setae on a loose mycelium, with distant hyphopodia. 56. ^Meliola panici Earle Muhlenbergia 1 : 12, 1901. On Panicum glutinosum Sw. Maricao, Nov. 18, '13, 4801, July 19, '15, 8934, July 20, '15, 8957. Monte de Oro, near Cayey, Dec. 3, '13, 5560, 5744, 5672, 5746. Utuado, Nov. 8, '13, 4389. El Alto de la Bandera, Nov. 8, '13, 4368, July 16, '15, 8930, 8680, 8647. Ponce, Nov. 8, '13, 4375. El Gigante, Dec. 15, '13, 5947. Las Marias, July 10, '15, 8187. On Paspalum sckreberianum (Fl.) Nash. Maricao, July 19, '15, 8803. 511] MELIOLA IN PORTO RICO — STEVENS 4S On Lasiacis compacta (Sw.) Hitch. Utuado, Nov. 8, '13, 4643, 4663. On Lasiacis divaricata (L.) Hitch. Arecibo, Jan. 17, 14, 6810. Manati, Nov. 5, '13, 4298. Vega Alta, Nov. 5, '13, 4195, 4189. Vega Baja, Nov. 5, '13, 45J7. Nov. 22, Arecibo, by Mrs. A. Chase. On Lasiacis swartziana, Hitch. Aiiasco, Oct. 12, '13, 3561. Las Marias, July 10, '15, 8191. On Oplismenus setarius (Lam.) Roem. & Schult. Maricao, July 19, '15, 8776. On Andropogon leucostachyus H. B. K. El Alto de la Bandera, July 15, '15, 8671. On Andropogon bicornis L. Las Marias, July 10, '15, 8168. Vega Baja, July 2, '15, 7751. On Olyra latifolia L. El Miradero, Aug. 4, '15, 9159. Mayaguez Mesa, June 25, '15, 7485^ 7486, June 29, '15, 7587, June 24, '15, 7390. Maricao, July 20, '15, 8959. San German, June 27, '15, 7518. Arecibo, Jan. 17, '14, 6805. On Chloris petraea Sw. Mayaguez, Aug. 1, '15, 7810. On Ichnanthus pallens (Sw.) Munro. Maricao, Apr. 3, '13, 829. Monte de Oro, Dec. 3, '13, 5755. Maya- guez Mesa, June 25, '15, 7441, 7485. On Gramineae indet, Monte de Oro, Dec. 3, '13, 5659. Arecibo, Jan. 17, '14, 6796. Tru- jillo Alto, Aug. 15, '15, 9441. The type was collected by Heller near Santurce, Porto Rico. The form on Panicum glutinosum and Ichnanthus is usually densely parasitized; the setae do not develop typically, nor are the capitate hyphopodia as likely to be angular as when on the other hosts listed. The mycelium upon Lasiacis swartziana, L. divaricata, Paspalum schreherianum, Olyra, Oplismenus, the Andropogons and Ichnanthus is frequently straight for great distances, but this I take to be due to the surface of the host rather than to a specific difference in the fungus. 57. Meliola rudolphiae sp. nov. Mycelium strictly epiphyllous in circular spots or diffuse, black. Mycelial setae abundant, long, 400//., black, tapering, septate, acute, sim- ple ; mycelium crooked. Capitate hyphopodia alternate, stalk cell short, 3-6/li, head cell ir- 44 . ILLINOIS BIOLOGICAL MONOGRAPHS [512 regular, usually bent to one side, 10 x lljx. AmpuUiform hyphopodia alternate, 7 x 21/x. Perithecia numerous, clustered, black, 150-170/x, slightly flattened, cup-shaped when dry, rough with low tubercles. Asei 4-spored, 65 x 27/i. Spores 4-septate, dark brown, ends rounded, constriction moderate, 48-51 X 17-20/*. On Rudolphia voluhilis Willd. Fig. 41. Monte Alegrillo, IMaricao, Nov. 14, '13, 4791 (type). Maricao, Nov, 18, '13, 4835. Luquillo Forest, Dec. 2, '13, 5439. El Alto de la Ban- dera, July 10, '15, 8698. Aibonito, July 16, '15, 8467. This species resembles M. montagnei, differing from it in color, spores, thickness, and density of mycelium. 58. Meliola sebjaniae sp. nov. Colonies hypophyllous, irregular, black, 1-6 mm. in diam. Mycelium forming a loose network of threads which branch irregularly, 6/i in diameter. Capitate hyphopodia small, alternate or irregular, distant, 40-70/x apart, head cell ovoid or pyriform and much lobed, 17 x 10/x, the basal cell 3-6/Lt. Mucronate hyphopodia usually more abundant than the capi- tate hyphopodia, opposite, bottle-shaped to conic, narrow, 24 x 6fi. Peri- thecial setae non^. Mycelial setae abundant, black throughout, straight or slightly curved, 280-800 x 10,u. Tip abruptly acute. Perithecia 150-190/x, smooth. Asci soon evanescent; ascospores 4- septate, brown, constricted, obtuse, 41 x 17/a. On Serjania polyphylla (L.) Rad. Fig. 42. Vega Baja, Feb. 22, '13, 425 (type). Florida Adentro, July 1, '15, 7654. Arecibo-Lares Road, June 21, '15, 7219. Catano, Nov. 6, '13, 4181. This form is clearly differentiated from M. parenchymate, M. am- higua, and M. thoniniae previously described on the Sapindaceae, all of which have simple mj-celial setae. The chief characters are angular, capitate hyphopodia, long, abruptly pointed setae, and numerous mucro- nate hyphopodia. 59. IVIeliola dipholidis sp. nov. Colonies amphigenous, irregular, circular, 2-5 mm. in diam. My- celium black, forming a loose network, 7(x in diameter, branches mainly opposite. Capitate hyphopodia small, alternate or irregular, not crowded, 1 per cell, cells about 21 fx long, head cell obovoid to pyriform or globular, often angular or bent, 17 x 10/t, the basal cell 5-7/i long. Mucronate 513] MELIOLA IN PORTO RICO — STEVENS 45 hyphopodia alternate or opposite, wedge to bottle-shaped, 18 x Ifi. Peri- thecial setae none. Mycelial setae few, straight or slightly curved, 400 x 7/A, dark throughout, tip acute. Perithecia llO-lSO/u,, slightly rough with rounded protuberances. Asci 2-spored, soon evanescent; ascospores 4-septate, brown, cylindrical, elliptical, distinctly thicker at the middle than toward the ends, strongly constricted, obtuse, 41-44 x 18/u,. On Dipholis salicifolia (L.) A.DC. Guayanilla, July 14, '15, 8549 (type). Quebradillas, June 20, '15, 7265. This species differs from M. amphitricha in shape of the capitate hyphopodia and spores and from M. brasiliensis in character of spores and setae. 60. Meliola ocoteicola sp. no v. Colonies amphigenous, irregular, 3-10 mm. in diam., confluent. Mycelium forming a rather loose network of threads, branches mostly opposite, IfjL in diameter. Capitate hyphopodia alternate, not crowded, about 30/x apart, cylin- drical, head cell scarcely thicker than the stalk, apex obtuse, the basal cell very short. Mucronate hyphopodia few and scattered, usually oppo- site, conical to bottle-shaped, 20 x 6/*. Perithecial setae none. Mycelial setae few, straight or slightly curved, 300-350/i,, dark, tip acute. Perithecia 150/t, ostiole none. Asci soon evanescent; ascospores 4- septate, brown, strongly constricted, obtuse, 40 x 17/ix. On Ocotea leucoxylon (Sw.) Mez. Fig. 43. Mayaguez, June 29, '15, 7560 (type). Monte Alegrillo, near Mari- cao, Nov. 14, '13, 4762. On Chrysophyllum sp. Monte Alegrillo, Nov. 14, '13, 4731 (type), Nov. 18, '13, 4519. In characters of the capitate hyphopodia this is similar to M. mag- noliae but is still clearly distinct from that form. Nos. 4579 and 4731 on Chrysophyllum present in the hypophyllous colonies a peculiar variation, in that the mycelium is exceedingly crooked and irregular. These represent, perhaps, a distinct species, but on ac- count of the general resemblance of the capitate hyphopodia and the setae to the other form of Chrysophyllum, they are regarded as co-spe- cific with it. 61. Meliola paulliniae sp. nov. Colonies black, setose, circular, epiphyllous, 3-8 mm. in diam. My- 46 ILLINOIS BIOLOGICAL MONOGRAPHS [514 celium forming a loose network of straight, radiating threads, 8/* thick, branches mostly opposite. Capitate hyphopodia alternate, often at right angles to mycelium, not crowded, 1 per cell, cells about 34/i long, head cell ovate, 17 x 14/t, stalk cell short. Mucronate hyphopodia usually opposite, bottle-shaped, neck narrow. Perithecial setae none. Mycelial setae abundant, straight, simple, black throughout, rigid, abruptly acute, 275-500 x 8-9/i. Perithecia 150-160)u,. Asci evanescent, spores 37-40 x 14-15/t, 4- septate, constricted, obtuse. On Paiillinia pinnata L. Mayaguez, May 3, '13, 1149 (type), Oct. 31, '13, 3956, 3914, 3967&. Vega Baja, Feb. 22, '13, 376. El Alto de la Bandera, July 16, '15, 8722. Rio Arecibo, K. 64.7, July 8, '15, 7787. Barros, Jan. 2, '13, 55. On Casearia ramiflora Vahl. Martin Pena, Aug. 11, '15, 9306, 9328. Barceloneta, Aug. 10, '15, 9256. Manati, July 2, '15, 7688. Vega Baja, March 1, '13, 512, July 2, '15, 7745, Nov. 5, '13, 4262, March 2, '13, 510. St. Ana, Dec. 31, '13, 6683. San German, Dec. 12, 13, 5844. On Casearia sylvestris Sw. Mayaguez Mesa, June 29, '15, 7566, June 14, '15, 7017, May 1, '13, 1051, May 4, '13, 1200. Miradero, Aug. 4, '15, 9136. Arecibo-Lares Road, June 21, '15, 7285. San German, Dec. 12, '13, 5837, 5864. On Casearia arhorea (L. CI. Rich.) Urb. Monte de Oro, Dec. 13, '13, 5709. On Casearia aculeata Jacq. Lajas, June 17, '15, 7151. On Casearia sp. Dos Bocas, below Utuado, Dec. '17, '15, 6071. Mayaguez, June 15, '15, 7074, Oct. 31, '13, 3935. 3920. The specimens on Casearia differ somew^hat from those on Paul- linia, especially in the character of the setae. On Mammea americana L. Maricao, Sept. 20, '13, 3641. Las Marias, July 10, '15, 8207. On host unknown, Martin Pena, Aug. 11, '15. The specimens upon Mammea show distinctly longer and more slender setae than those on other hosts and are perhaps specifically dis- tinct. According to the keys of Gaillard this species would be identified as M. polytricha, K. and C, therefore, for purposes of comparison, I am publishing a photograph of the type of M. polytricha, fig. 44. 515] MELIOLA IN PORTO RICO — STEVENS 47 62. Meliola chamaecristae Earle BuU, N. Y. Bot. Gard. 3 : 304, 1905. Described "on Chamaecrista glandulosa," collected by Heller, No. 6371. 63. Meliola earlii sp. nov. Colonies amphigenous, irregular, 1-4 mm. in diam. Mycelium form- ing a loose network of crooked threads which branch irregularly, often angling at the hyphopodia, about 6fx in diam. Capitate hyphopodia small, alternate, not crowded, about 25/t apart, ovoid or pyriform, sometimes angular or bent, 8 x 10/x, the basal ceU short. Mucronate hyphoodia few, bottle-shaped. Perithecial setae none. Mycelial setae few or numerous, about 160/x, long, straight or slightly curved, opaque, tip acute. Perithecia 160/x, rough with rounded protuberances, ostiole none. Asci soon evanescent ; ascospores 4-septate, brown, somewhat constricted,, obtuse, 35-40 x lO/u,. On Pilea sp. Fig. 45. Florida Adentro, July 1, '15, 7685 (type). On Pilea parietaria (L.) Bl. Rio Arecibo K. 64.7, July 8, '15, 7804. On Pilea nummularifolia (Sw.) Wedd. Jajome Alto, Dec. 3, '13, 5640. The species is associated with a Meliola determined by Earle as M. iriloha on Pilea parietaria. Heller, No. 558. It is differentiated from M. leptospora by spore characters. This Meliola though associated with M. triloba is clearly distinct from it in the general habit of the mycelium and especially in the presence of mycelial setae on M. earlii. The species is named in honor of F. S. Earle in recognition of his researches upon Porto Rican fungi. 64. Meliola gesneriae sp. nov. Colonies hypophyllous, circular, 2-8 mm. in diam. Mycelium black, forming a close network, 7/t in diameter, branches mostly opposite. Capitate hyphopodia alternate, not crowded, 1 per cell, cells about 24/x, long, head cell ovate to globular, 10 x 14fi, the basal cell 4-6/i long. Mucronate hyphopodia alternate or opposite, bottle-shaped. Perithecial setae none. Mycelial setae straight or slightly curved, 400 x Ifi, dark throughout, most abundant around the perithecia. Tip acute. Perithecia 110/x, smooth. Asci soon evanescent ; ascospores 4-septate, brown, very slightly constricted, 41 x 17/m. -48 ILLINOIS BIOLOGICAL MONOGRAPHS [516 On Gesneria alhiflora (Dene.) O. Ktz. Mayaguez Mesa, June 25, '15, 7431 (type), 7465. Dos Bocas, below Utuado, July 8, 15, 8018, Dee. 30, '13, 6590. On Cestrum laurifolium I'Her. Maricao, Apr. 3, '13, 824. On Cestrum macrophyllum Vent. El Alto de la Bandera, July 14, '15, 8301. El Gigante, July 16, '15, 8561. 65. Meliola jatrophae sp. nov. Colonies epiphyllous, circular to irregular, 1-4 mm. in diam. My- celium forming a loose network of threads; branches mostly opposite, 7fi in diameter. Capitate hyphopodia alternate or irregular, forming an acute angle with the mycelium, not crowded, about 21 fi apart, head cell ovate, 17 x Ifi, apex obtuse, the basal cell short. Mucronate hyphopodia abundant and crowded on certain threads, opposite, bottle-shaped. Perithecial setae none. Mycelial setae few, except around the base of the perithecia where they are numerous, curved, about 250/x long, dark, tip acute. Perithecia 130-150/x, slightly rough, with rounded protuberances, Asci soon evanescent; aseospores cylindrical, 4-septate, brown, slightly constricted, obtifse, short, thick, 30 x IT/x. On Jatropha hernandifolia Vent. Rio Tanama, near Arecibo, July 6, '15, 7873 (type). Dos Bocas, near Utuado, July 8, '15, 7930. The most distinctive characters are the short, thick aseospores and the ovate, capitate hyphopodia, all directed forward at an acute angle. 66. Meliola mayepeae sp. nov. Colonies amphigenous, more commonly epiphyllous, circular, 1-5 mm. in diam. Mycelium black, forming a rather close network, 7/* in diameter, branches opposite, mostly at acute angles. Capitate hyphopodia small, alternate, not crowded, 1 per cell, cells about 35/x long, head cell obovoid, 10 x 7/x, the basal cell Sfi long. Mu- cronate hyphopodia not seen. Perithecial setae none. Mycelial setae few to abundant, most plentiful around the perithecia, slightly curved, about 250 x 6fx, dark throughout. Tip simple, obtuse. Perithecia 170/i,, slightly rough. Asci soon evanescent; aseospores 4-septate, brown, very slightly constricted, obtuse, 30-35 x 17/a. On Mayepea domingensis Krug and Urb. Fig. 46. Mayaguez Mesa, June 25, '15, 7468 (type). El Alto de la Bandera, July 16, '15, 8703. 517] MELIOLA IN PORTO RICO — STEVENS 49 This species is distinguished from M. brasiliensis by the shape of the capitate hyphopodia and the bases of the setae. 67. Meliola gymnanthicola sp. nov. Colonies epiphyllous, circular, 2-4 mm. in diam. Mycelium form- ing a close network, 7/u, in diameter, branches mostly opposite and often at right angles. Capitate hyphopodia alternate, not crowded, 1 per cell, cells about 20/A long, head cell ovoid to nearly cylindrical, 17 x lO/x, the basal cell 2-Zfi long. Mucronate hyphopodia alternate or opposite, conical to bot- tle-shaped, irregular, 16-18- x 7fi. Perithecial setae none. Mycelial setae scattered, slightly crooked, 300 x 7fi. Tip obtuse, dark. Perithecia 190/i, slightly rough. Asci soon evanescent ; ascospores 4-septate, brown, very slightly constricted, obtuse, 37 x 17 fi. On Gymnanthes lucida Sw. Guayanilla, July 14, '15, 8596 (type). 68. Meliola lucumae sp. nov. Colonies amphigenous, much larger below, irregularly circular, ^-10 mm. in diam., diffuse. Mycelium forming a loose network, 7/a in diameter, branches mostly opposite. Capitate hyphopodia alternate, distant, 1 per cell, cells about 35/a long, head cell cylindrical or tapering slightly at each end, 17 x 7/m, the basal cell 3/x long. Mucronate hyphopodia few, mostly opposite, narrow-conic, about 20 x 4/*. Perithecial setae none. Mycelial setae few slightly curved, 200-800 x 7/*, dark throughout. Tip obtuse. Perithecia 100-150)ii, slightly rough with rounded projections. Asci soon evanescent; ascospores 4-septate, pale brown, slightly contricted, obtuse, 44 x 18)u,. On Lucuma multiflora A. DC. Fig. 47. Las Marias, July 10, '15, 8164 (type). The distinctive character of the species is the sparse, diffuse, my- celium, with cylindrical, capitate hyphopodia associated with slightly differentiated mucronate hyphopodia. Guayanilla, July 14, '15, 8596 (type). 69. Meliola amphitrichia Fr. Elench. Fung. 2 : 109, 1828. Eeported by Earle *'on leaves of tree." Heller collection. No. 288 near Caguas, 1899. 70. Meliola byrsonimae sp. nov. Colonies epiphyllous, circular to irregular, 2-5 mm. in diam. My- <;elium forming a loose network of threads, IO/a in diam. so ILLINOIS BIOLOGICAL MONOGRAPHS [518 Capitate hyphopodia alternate, distant, usually about 120/1, apart, cylindrical, 20 x 17/*, often irregular or curved, the basal cell 3-5/i long. Mucronate hyphopodia opposite, bottle-shaped, about 24/* long. Perithecial setae none. Mycelial setae very few, scattered, straight or slightly curved, lOOO/i, long, dark throughout. Tip obtuse to acute. Perithecia 150 to 160/x, smooth, subtended when young by an areola of radiate hyphae. Asci soon evanescent; ascospores 4-septate, brown, slightly constricted, obtuse, 54 x 18/x. On Byrsonima lucida (Sw.) L. CI. Eich. Guayanilla, July 14, '15, 3541 (type). This is closely related to M. amphitricha, M. amhigua and M. coral- Una, differing from the last two in spore size ; differing from the first, in abundance, length and color of setae, and in character of capitate hyphopodia and mycelium. 71. Meliola tabernaemontanae Speg. Ann. d. Mus. Nac. de Buenos Aires ; 33 : 42, 1912. On Rauwolfia nitida Jacq. Martin Pena, Aug. 11, '15, 9327, 9300. On Tahernaemontana oppositifolia (Spreng.) Urb. Maya^ez, June 15, '15, ?]073. Hormigueros, K. 7, June 23, '15, 7352. Mayaguez Mesa, June 29 ,'15, 7558. On Plumiera krugii Urb. Maricao, July, 19, '15, 8818. These specimens conform perfectly with type material kindly sup- plied by Dr. Spegazzini. The most distinctive characters are the regularly shaped, capitate hyphopodia and the black, truncate setae. 71a. Meliola tabernaemontanae. var. forsteroniae var. nov. The specimens on Forsteronia agree with the type quite closely, but present constant differences in the setae and capitate hyphopodia as follows : The setae are long, about 700/x, and taper to an acute apex. The capitate hyphopodia are larger and more angular than in the type. On Forsteronia corymhosa (Jacq.) Mey. Utuado, 4682 (type). 72. Meliola ambigua Pat. and Gaill. Bull Soc. Myc. de Fr. 4: 104, 1888. On Lantana odorata L. Quebradillas, June 20, '15, 7268, 7267. 519] MELIOLA IN PORTO RICO — STEVENS 51 On Lantana camara L. Dos Bocas, below Utuado, July 8, '15, 8016, 8025. Eio Arecibo, K. 64.7, July 8, '15, 7806. On Lantana sp. Quebradillas, Nov. 22, '13, 5008. Dos Bocas, below Utuado, Dec. 17, '13, 6052, Dec. 17, '30, '13, 6870. 73. Met .TOLA pstchotriae Earle Bull. N. Y. Bot. Gard. 3: 308, 1905. On Erithalis fruticosa L. Quebradillas, Aug. 10, '15, 9240, 9229. Mona Island, Dec. 20, '13, 6082, 6138, 6254. Guayanilla, July 14, '15, 8599. Also collected by Heller on this host, Jan. 19, 1900, No. 6430, and on Psychotria sps., near Ponce, Dec. 9, 1902. On Gonzalagunia spicata (Lam.) G. Maza. Sabana Liana, Aug. 13, '15, 9371. Miradero, Aug. 4, '15, 9134. Mayaguez, June 24, '15, 7388, July, '15, 7910, June 14, '15, 7044, 7046. Vega Baja, Aug. — , '15, 9271. Eio Arecibo, K. 64.7, July 8, '15, 7793. Mayaguez Mesa, June 29, '15, 7592. On Randia aculeata L. Quebradillas, June 20, '15, 7301, Nov. 22, '13, 4985. Florida Aden- tro, July 1, '15, 7757. Hormigueros, June 23, '15, 7351. Monacillo, Aug. 12, '15, 9353. The specimens on this host show slight differentiation in the hypho- podia and setae. On Chdococca alba (L.) Hitch. Mayaguez Mesa, June 25, '15, 7467. Rio Tanama, July 6, '15, 7859. Martin Peiia, Aug. 11, '15, 9299. Hormigueros, June 23, '15, 7325. On Guettarda ovalifolia Urb. Maricao, Jan. 10, '12, 234. On Borreria laevis (Lam.) Griseb. El Alto de la Bandera, July 14, '15, 8593, 8598. Dos Bocas, below Utuado, July 8, '15, 8090. On Borreria ocimoides DC. One specimen with no data. On Ruhiaceae indet. Guayanilla, July 14, '15, 8550, 8546. I find this species also on Exostema caribaceum (Jacq.) R. and Sch. collected in Santo Domingo by Taylor, No. 483. 74. Meliola matepeicola sp. nov. Colonies epiphyllous, circular, punctiform, 1-2 mm. in diam. My- 52 ILUNOIS BIOLOGICAL MONOGRAPHS [520 celium black, forming a very close network, 9/i in diameter, branches mostly opposite. Capitate hyphopodia large, alternate, close, 1 per ceU, cells about 20/1 long, head cell pyriform, 24 x 17/i, the basal 7/x long. Mucronate hyphopodia few, alternate or opposite, bottle-shaped, crooked, narrow, 16-18 X 6/1. Perithecial setae none. Mycelial setae few, straight or slightly curved, 150 x 7/i, dark throughout, tip obtuse. Perithecia lOO-119/i, slightly rough, with conic projections. Asci soon evanescent; ascospores 4-septate, brown, very slightly constricted, obtuse, 34 x 14/i. On Mayepea domingensis Krug and Urb. Mayaguez Mesa, June 29, '15, 7556 (type). Maricao, Apr. 3, '13, 822. The distinctive characters of this species are close, dense, small colony and thick capitate hjT)hopodia. The setae are sometimes very scant and may perhaps be entirely wanting. ^Most leaves which bear this fungus are infested with M. mayepeae also, but the two are readily separable by the naked eye. 75. I^Ieliola clusiae sp. nov. Colonies hypophyllous, irregular, diffuse, 3-5 mm. in diam. Mycelium brown, 8/i thick, nearly straight, branching mostly opposite. Capitate hyphopodia alternate, 1 per cell, cells about 30/i long. Head cell ovate to elliptical, more rarely cylinder or irregular, 20 x 14/i, stalk cell 2-4/t long. Mucronate hyphopodia scattered, 20 x 7/i, bottle- shaped. Perithecial setae none. Mycelial setae few, 800 x lO/i at base, tips obtuse, brittle, usually broken, mostly clustered around the peri- thecia. Perithecia apparently dimidiate, with an areola of radiating hyphae. Spore 61 x 20/i, strongly constricted, obtuse, cylindrical. On Clusia minor L. El Alto de la Bandera, July 15, 8571, July 15, '15, 8283 (type). Section F. Spores 4-septate, no perithecial setae or appendages, mycelial setae simple, uncinate. There are only two species known in Porto Rico. Key To Species of Section F. Capitate hyphopodia crowded M. guareieola No. 76 Capitate hyphopodia not crowded M. tecomae No. 77 521] MELIOLA IN PORTO RICO — STEVENS 53 76. Meuola guareicola sp. nov. Colonies epiphyllous, circular, punctiform, 1-4 mm. in diam., black. Mycelium dense, forming a close mat of threads which readily sepa- rates as a whole from the substratum. Capitate hyphopodia alternate or irregular, crowded, head cell globular, ovoid or pyriform, 14 x 10/i, the basal cell 2-3/t long. Mucro- nate hyphopodia alternate or opposite, bottle-shaped, the neck narrow, 16-18 X 7fi. Perithecial setae none. Mycelial setae numerous, forming- a dense mat covering the colony to the edge, 200-300/i long, 7/t thick,, dark, pale at tip, upper part strongly coiled or twisted. Tip acute. Perithecia small, 100-500;^, distinctly ostiolate. Asci soon evanes- cent ; ascospores 4-septate, brown, slightly constricted, obtuse, 37 x lO/i. On Ouarea trichilioides L. Las Marias, July 10, '15, 8166 (type), July 11, '15, 8245. Maya- guez Mesa, June 25, '15, 7464. Ad juntas, Nov. 22, '13, 4971. Monte de Oro, near Cayey, Dec. 3, '13, 5737. Dos Bocas, below Utuado, July 8 '15,8096. ^ ^ This species is usually associated with M. guareae from which it is readily distinguished by its uncinate hairs. 77. Meliola tecomae sp. nov. Colonies amphigenous, mainly epiphyllous, circular, 2-5 mm. in diam. Mycelium black, forming a close network of threads, l/x in diame- ter, branches mostly opposite. Capitate hyphopodia alternate, not crowded, 1 per cell, cells> about 35/Li long, head cell ovoid, 17 x 13/t, the basal cell 6-10/u. long. Mucronate hyphopodia opposite, irregularly conic, 16-18 x 6fi, no well differentiated neck. Perithecial setae none. Mycelial setae abundant, broadly curved into a large hook at the end, 170 x lOfx, dark throughout, tip obtuse. Perithecia 170^, slightly rough with rounded protuberances. Asci soon evanescent; ascospores 4-septate, brown, very slightly constricted, obtuse, 44 x 17/t. On Tecoma pentaphylla (L.) Juss. Fig. 48. Martin Pena, Aug. 11, '15, 9332 (type), Mayaguez, June 24, '15, 7396, 7078. Las Marias, July 10, '15, 8177. Maricao, July 20, '15, 8960. On Tecoma sp. El Miradero, Aug. 4, '15, 9163. Las Marias, March 22, '13, 359S, Mayaguez, Oct. 31, '13, 3950. Maricao, Nov. 18, '13, 4804. Quebra- dillas, Nov. 22, '13, 4978, 4981. Vega Baja, Nov. 5, '13, 43108i. Arecibo- Lares Road, Jan. 21, '14, 6790. The distinguishing character is the long, broadly curved or hooked^ 54 ILLINOIS BIOLOGICAL MONOGRAPHS [522 black, setae. They, in general, resemble those figured by Gaillard for M. halansae or M. intermedia, but the agreement goes no further. There is a strong superficial resemblance to M. pazschkeana, but the two are readily distinguished by their very different mucronate hyphopodia. A specimen on Tecoma which I have not seen, labelled M. pazsch- keana, in the Bronx herbarium may possibly belong here. Section G Spores 4-septate, no perithecial appendages, mycelial setae both, simple and forked. Key to Species of Section G^ Capitate hyphopodia opposite M. ipomoeae No. 78 Capitate hyphopodia not usually opposite Setae very long, 1000/x M. guareae No. 79 Setae shorter Capitate hyphopodia very narrow, scarcely thicker than the stalk cell M. magnoliae No. 80 Capitate hyphopodia thicker Head cell nearly globular M. bicornis No. 95 Head cell more or less elongated Capitate .hyphopodia often opposite M. smilacis No. 81 Capitate hyphopodia alternate Head cell regular M. helleri No. 82 Head cell irregular Setae sometimes forked M. mangiferae No. 83 Setae merely denticulate M. denticulata No. 84 78. IVIeliola ipomoeae Earle (Not M. ipomoeae Kehm-) Muhlenbergia 1 : 10, July 1901. On Ipomoea cathartica Poir. Las Marias, July 10, '15, 8183. Yega Baja, July 2, '15, 7729. El Alto de la Bandera, July 16, '15, 8692. On Ipomoea tUiacea (W.) Choisy. Anasco, Oct. 12, '13, 3506. Mayaguez, Oct. 31, '13, 3909. On Ipomoea sps. Fig. 49. El Miradero, Aug. 4, '15, 9160. Dos Bocas, below Utuado, July 8, ^M. stenotaphri which very rarely has bifid setae is to be found in section R. ^The use of this specific name by Rehm (Ann. Myc. 12: 171, 1914) is clearly invalidated by the prior use by Earle and M. ipomoeae Rehm becomes M. ipomoeae- phile Rehm, in lit. 523] MELIOLA IN PORTO RICO — STEVENS IS '15, 8083. Dee. 30, '13, 6563. Rosario, No. 4810. Marieao, July 19, '15, 8784, 9001. The type was collected near Mayaguez by Heller, No. 4358, in 1900. A specimen from the Bronx Garden, No. 6258, determined by Earle, shows the capitate hyphopodia to be almost exclusively opposite. Al- though this character is not mentioned by Earle in his description it appears to me to be the most distinctive feature of the species. The perithecia instead of being * ' few, 2-4, ' ' as described, are very numerous, 20+ per colony. The forward angling of the hyphopodia appears to me to be a variable character. This fungus frequently grows upon the same leaf with M. clavulata which it resembles very closely, with the exception of the setal tips and the arrangement of the capitate hyphopodia. 79. Meliola guareae Speg. Ann. d. Mus. Nac. de., Buenos Aires, 23 : 42, 1912. On Guarea trichUioides L. Las Marias, July 10, '15, 8166 (type). Dos Bocas, below Utuado, July 8, '15, 8096. Monte de Oro, Dec. 3, '13, 5737. Adjuntas, Nov. 22, '13, 4971. Jajome Alto, Dec. 3, '13, 5691. Mayaguez Mesa, June 25, '15, 7464. Usually associated with M. guareicola but on the lower side of the leaves. These specimens differ from the type, which was sent to me by Dr. Spegazzini, in that they have larger hyphopodia, more abundant setae, and in minor colony characters, especially in density of growth. The setae with forked tips are rare and the fact that this character was not mentioned by Spegazzini is not significant. 80. Meliola magnoliae sp. nov. Colonies hypophyllous, irregular, 3-20 mm. in diam., black. My- celium forming a very loose network of threads, 7/* in diameter. Capitate hyphopodia alternate or irregular, distant, about 45/i apart, head cell cylindrical, very little thicker than the stalk cell, straight or somewhat curved, obtuse, the basal cell about 7/u. long. Mucronate hyphopodia opposite or irregular, almost cylindrical to slightly bottle-shaped, 20/t long. Perithecial setae none. Mycelial setae few, about 700/* long, straight or slightly curved, opaque. Tip acute, simple or occasionally forked, with short teeth. Perithecia 200/i, in diam., slightly rough with rounded protuber- ances, ostiole none. Asci soon evanescent; ascospores 4-septate, dark brown, very strongly constricted, obtuse, 51 x 20/t. 56 ILLINOIS BIOLOGICAL MONOGRAPHS [524 On Magnolia porioricensis Bello. Fig. 50. Monte Alegrillo, near Maricao, Nov. 14, '13, 4738 (type). This species is remarkable for the slight differentiation of its capi- tate and mucronate hyphopodia which differ from each other but little and also verj^ much resembles branches, though of determinate growth. This is evidently one of the most primitive forms in the genus. 81. Meliola smilacis sp. nov. Colonies amphigenous but more abundant above, circular, irregular, 2-4 mm. in diam. Mycelium not crooked, of medium density, branches opposite. Capitate hyphopodia alternate, opposite or unilateral, head cell cylindrical to globular, 14 x llfi, stalk cell short, about 4/x. Mucronate hyphododia rare, conical, neck short and tapering, or ampulliform with neck longer. Mycelial setae abundant, about 500/t high, tapering, lO/x. thick at base, apex obtuse, rarely bifid, pale, base opaque. Perithecial setae or appendages none. Perithecia about 200/i in diam. Ostiole a mere thinning of the apical region. Asci soon evanes- cent, short type. Spores 4-septate, but slightly constricted, ends ob- tuse, 15 X 17/1. On SmUax coriacea Spreng. Manati, Nov.. 25, '13, 5261 (type). On SmUax sp. indet. Jajome Alto, July 17, '15, 8429. The two hosts appear to be quite different but it is possible that the latter specimen is a form of S. coriacea. This Meliola belongs to the group of M. amphitricha from which it is distinguished by its ostiole, which is however, very difficult of ob- servation, by the sometimes forked tips of the mycelial setae and by the capitate h}T)hopodia. 82. Meliola helleri Earle Bull. N. Y. Bot. Card. 3 : 307, 1905. The type is on an "unknown woody plant perhaps belonging to the Myrtaceae", Heller No. 6251, 3 miles west of Ponce, Dec. 9, 1902. The setae in the cotype specimen which I examined were more fre- quently simple than forked as called for in the description. On Eugenia stahlii (Kiaer.) K. and Urb. Luquillo Forest, Dec. 4, '13, 5343. El Alto de la Bandera, July 15, '15, 8665. Jajome Alto, July 17, '15, 8436. The specimens are much overgrown by parasites. Setae were seen upon one specimen, but all were simple. 525] MELIOLA IN PORTO RICO — STEVENS 57 On Eugenia monticola (Sw.) P. DC. Manati, Nov. 5, '13, 4285. On Myrcia deflexa (Poir) P. El Alto de la Bandera, July 14, 15, 8268, 8268, 8672. This collection is heavily overgrown by parasites and the determina- tion is therefore not fully reliable. The typical setae were not seen but the character of the mycelium and capitate hyphopodia were those of M. helleri. On Myrcia splendens (Sw.) P. DC. Jajome Alto, Dee. 3, '13, 5646. 83. Meliola mangiperae Earle Bull. N. Y. Bot. Card. 3: 307, 1905. On Mangifera indica L. Mayaguez, Aug. 5, '15, 9478, Jan. 8, '14, 6723, June 16, '15, 7109. Vega Baja, Feb. 23, '13, 440. Manati, Nov. 5, '13, 4300. Luquillo Forest, Dec. 2, '13, 5558, 5428. El Gigante, near Adjuntas, Dec. 15, '13, 6016. Collected also by Heller, No. 6393, near Rio Piedras, Jan. 9, 1903. In many cases the colonies, particularly epiphyllous colonies, lack the usual velvety appearance, have few setae, a loose mycelium instead of the dense mycelium, and lack the numerous hairs that characterize the large black hypophyllous blotches. The forking of the setae seems to be the exception rather than the rule. 84. Meliola denticulata Wint. Gaillard, Le Genre Meliola : 98, 1892. On Boystonea horinquena Cook. Arecibo-Lares Road, June 21, '15, 7279. Section H Spores 4-septate, no perithecial appendages, mycelial setae forked. Key to Species of Section H Apical branches strongly divergent, well-developed Primary branches at nearly right angles Primary branches long, 15-|-/i Secondary branches well developed M. cucurbitacearum No. 85 Secondary branches merely toothed M. furcata No. 86 Primary branches short, 4-10/i. M. hessii No. 87 Primary branches not at right angles 58 ILLINOIS BIOLOGICAL MONOGRAPHS [526 Primary branches short, 18/i M. piperis No. 88 Primary branches longer Capitate hyphopodia mostly opposite Secondary and tertiary branches well developed M. quadrispina No. 89 Tertiary branches often not developed M. philodendri No. 90 Capitate hyphopodia alternate M. merrillii No. 91 Apical branches merely teeth or not strongly divergent Apical teeth crowded, forming a crest M. gaillardiana No. 92 Apical teeth not crowded to form a crest Setae very dark Setae about 400/i, long, hyphopodia largely opposite M. dieffenbachiae No. 93 Setae about 300/i, long M. bidentata No. 94 Setae translucent M, bicornis No. 95 85. Meliola cucurbitacearum sp. nov. Colonies epiphyllous, circular, 1-3 mm. in diam. On leaves and stems. Mycelium black, forming a rather close network, 7/t in diameter, branches mostly alternate. Capitate hyphgpodia alternate, not crowded, 1 per cell, cells about 17 to 24/A long, head cell ovoid, lobed, 17 x 14/x,, the basal cell 7/t long. Mucronate hyphopodia alternate or opposite, bottle-shaped, 20 x 5/*, neck narrow and crooked. Perithecial setae none. Mycelial setae abun- dant in the older parts of the colony, 190 x 9/u,, dark throughout. Tip once or twice dichotomous, branches standing at nearly right angles to the main axis, primary branches 15-35/1, secondary about the same, apices acute. Perithecia 120/x, rough with rounded protuberances. Asci soon evanescent ; ascospores 4-septate, brown, slightly constricted, obtuse, 41 X 14/Li. On leaves and stems of an unknown Cucurb, probably Cayaponia. El Alto de la Bandera, July 16, '15, 8732 (type). Fig. 51. The species is readily recognized by its characteristic, once or twice dichotomous setae and its lobed capitate hyphopodia. 86. Meliola furcata Lev. Ann. Sc. Nat. 266, 1846. On Coccothrinax alta (Cook) Becc. Dos Bocas, below Utuado, Dec. 17, '13, 6060. On Acrista monticola Cook. El Alto de la Bandera, 8303 a. LuquiUo Forest, Dec. 4, '13, 5400. 527] MELIOLA IN PORTO RICO — STEVENS 59 On Thrinax ponceana Cook. Guayanilla, July 14, 15, 8590, 8017, July 26, '15, 9074. On Thrinax praeceps Cook. Dos Bocas, below Utuado, July 8, 15, 8017. On Macrodiscus lactiflorus (Vahl.) Bur.^ Coamo, Apr. 6, 13, 850 a. 87. Mfjjola HESsn sp. nov. Colonies epiphyllous, irregular, orbicular, 2-3 mm. in diam. Cen- ters denuded when old. Mycelium straight, forming a close network of threads, branches mostly opposite, 7/x in diameter. Capitate hyphopodia opposite, crowded, about ll/x apart, head cell pyriform, 7 x 10/i, the basal cell 3/* long. Mucronate hyphopodia oppo- site, bottle-shaped. Perithecial setae none. Mycelial setae erect, rigid, not very numerous, uniformly about 190ju, high, 8/t thick. Tip divided dichotomously, once, twice or thrice, into short branches which stand out nearly at right angles to the main stalk. Setal branches about 15/* long. Perithecia 150/*, minutely rough. Asci soon evanescent, 4-spored; ascospores 4-septate, brown, slightly constricted, obtuse, 41 x 14/*. On Paullinia pinnata L. Fig. 52. Sabana Liana, Aug. 13, 15, 9367 (type). .Mayaguez, May 4, 13, 1207\i. Named in honor of Mr. W. E. Hess who collected largely in Porto Rico. The species is remarkable for its straight, rigid setae of character- istic branching. Its characters are so marked that it is separated readily by the naked eye from the other species on Paullinia. 88. Meliola piperis Earle Muhlenbergia 1 : July 1901, 12. On Piper adunctum L. Dos Bocas, below Utuado, July 7, 15, 7964. Las Marias, July 10, ^The determinations of the Meliolas on Palms were all made by Mr. W. E. Pickler, who has made a special study of the variability of the setal tips of M. furcata. tie ILLINOIS BIOLOGICAL MONOGRAPHS [528 '15, 8603. Maricao, April 3, '13, 758. Monte de Oro, near Cayey, Dec. 3, '13, 5678. The type was collected by Heller on P. adunctum near Mayaguez, Jan. 1900, No. 4359 b. 89. Meliola quadrispina Rac. Parasit. Algen. u. Pilze, Javas, III, 33, 1900. Meliola quadrifurcata Rehm. Leaflets of Philippine Bot, 6: 2194, 1914. On Ipomoea cathartica Poir. Fig. 53. Las Marias, July 10, '15, 8163. • In general aspect of the colony and in the shape of the hyphopodia there is a striking similarity among the three species above recorded on the Convolvulaceae, which may point to a close relationship between them. This very beautiful species differs from all others that I have seen in its delicately forked setae, which are indeed the striking charac- ters of the colony. Both types of hyphopodia are strictly opposite though this character is not mentioned in the original description. 90. Meliola philodendri sp. nov. Colonies chiefly epiphyllous, circular, black, 1-5 mm. in diam., velvety. Mycelium coarse, 8/x,, straight, branches opposite at uniform angles, setigerous close to the margin of the colony. Capitate hyphopodia mostly opposite, about 20-30/a apart, stalk cell short, Sfi, head cell oval, not angular, 10 x 14)u,. Mucronate hyphopodia scarce, ampuUiform, alternate or opposite, often crowded, mycelial setae long, 230-300/i., black, at base about 9/i thick, dichotomous, forking three or more times, ultimate branches acute, branches about 120ju, long. Perithecia about 185/a in diam. Spores cylindrical, obtuse, 4-sep- tate, only slightly constricted, 48-54 x 15-20/x,. Spores sometimes nar- rower and much constricted, becoming moniliform in appearance. On Philodendron krehsii Schott. Fig. 54. Arecibo-Lares Road, June 21, '15, 7225 (type). Jayuya, March 2, '13, 377, Mch. 1, '13, 437. Ponce, Nov. 8, '13, 4346. Jajome Alto, July 17, '15, 8424. Maricao, July 20, '15, 8994. El Alto de la Bandera, July 16, '15, 8712. The long, branching, dichotomous setae, together with the opposite hyphopodia distinguish this species. The species is a conspicuous one and is of common occurrence in Porto Rico wherever the host is found. 529] MELIOLA IN PORTO RICO — STEVENS 61 91. MetjoLxN. merrillh Syd. PhU. Jour. Sc. 8 C 6: 479, 1913. On Cissus sicyoides L. San German, Dec. 12, '13, 5846, Nov. 8, 13, 5789, 5788. Lares, Nov. 22, '13, 4841. Utuado, Nov. 8, '13, 4418, 4398. Villa Alba, Jan. 3, '12, 101. El Gigante, near Adjuntas, Dec. 15, '13, 5819. Dos Bocas, below Utuado, Dec. 17, '13, 6063, July 8, '15, 8101, July 7, '15, 7968. Anasco, Oct. 12, '13, 3565. Aguada, Nov. 22, '13, 5102. Yauco, Oct. 3, '13, 3145, 3143. Mayaguez, Oct. 31, '13, 3948, 3910. Rio Tanama, July 6, '15, 7913. Manati, July 2, '15, 7690. The type is on Cissus leaves collected in Luzon by Merrill. A co- type specimen was kindly sent to me by Doctor Merrill, also by Dr. H. Sydow, and comparison clearly verifies the determination, though there are certain minor differences. In particular, the mycelium in the Porto Rican specimens is much more dense and luxuriant. 92. Meliola gaillardiana sp. nov. Colonies epiphyllous, small, circular, 1-2 mm. in diam., black; my- celium irregular, crooked, forming a very close, dense network of threads about 9/i, thick, cells about 14/li long. Capitate hyphopodia small, alternate or irregular, crowded, about 7-17/Li apart, ovoid, pyriform or curved, 10/x in diam., the basal cell 5-6ju, long. Mucronate hyphopodia few and scattered, alternate or opposite, bottle-shaped, the neck narrow. Mycelial setae abundant, straight, rigid, 220/i long, 10/i, thick, opaque; at tip bearing a crest, about 17-34/x, of short, acute spines ; sometimes forking below this crest. Perithecial setae none. Perithecia 170/x in diam., slightly rough with rounded protuber- ances of small, 6/i,, subprominent cells, ostiole none. Asci soon evanes- cent; ascospores 4-septate, brown, very slightly constricted, obtuse, ter- minal cells longer than the others, 3 x 14/t. On Piper adunctum L. Fig. 55. Rio Arecibo, July 8, '15, 7794 (type), 7796. Dos Bocas, below Utuado, July 8, '15, 8044, Dec. 30, '13, 6802. Las Marias, July 11, '15, 8223. The crests at the apices of the mycelial setae are very distinctive. They seem to be due to a very close, dichotomous branching. The form is most nearly related to M. piperis but is separated from that species by the setal tips and the density of mycelium. It differs much from M. pululahuensis in its mycelial tips. The difference from M. patouUlardi is less, still the form appears to be distinct from both. 62 ILLINOIS BIOLOGICAL MONOGRAPHS [530 Named in honor of A. Gaillard in recognition of his extensive studies of the genus Meliola. 93. Meliola dieffenbachl^ sp. nov. Colonies amphigenous with a tendency to be more numerous above but larger below, 1-5 mm. in diam,, black, velvety. Mycelium branches usually opposite at uniform angles forming a rather dense mat. Capitate hyphopodia alternate or opposite, oblong, spherical or curved, basal cell 4/* long, head cell 10-14/Lt. Mucronate hyphopodia few, alternate or opposite. Mycelial setae rigid, black, straight, about 400/a long, short branched or merely toothed apex. Teeth of varying number and size. Perithecial setae or appendages none. Asei soon evanescent, 2-4-spored. Spores cylindrical, 40 x 14ju,, 4-septate, ends obtuse, slightly constricted at the septa, end cells rather larger than the others. Perithecia grouped in center of colony, numerous, 140-170/t in diam. On Dieffenhachia sequine (Jacq.) Schott. Fig. 56. Las Marias, July 10, 15, 8148 (type), 8210. Maricao, Oct. 18, '13, 3889, July 19, '15, 8851. Catano, July 2, '15, 7707. Lajas, June 17, '15, 7155. Monte de Oro, near Cayey, Dec. 3, '13, 5666, 5731. Dos Bocas, below Utuado, July 8, '15, 8074, 8077. Mayaguez, June 24, '15, 7420. This differs strikingly from M. phUodendri in the branching of its setae. It resembles M. fuscidula but differs from it in the character of the setae. 94. Meliola bidentata Cke. Grev. 11 : 37, 1882. . On Tecoma pentaphylla (L.) Juss. Guanajibo, June 19, '15, 7202, 9002. Hormigueros, K. 7, June 23, '15, 7347. On Tdbebuia haemantha (B.) Gris. Monte Alegrillo, 4716. On unknown dicot, St. Ana, July 1, '15, 7621, 7633. The above named specimens were compared with and agree with a specimen obtained from the Kew Gardens. (Ravenel Fung. Amer. Exs. — ^No. 330,) there labeled ''M. furcata, Lev." but also labeled in Cooke's writing **not furcata Lev. but bidentata Cke. M.C.C." 95. Meliola bicobnis Wint. Hedw. 26 : 99, 1886. On Meihomia axillaris (Sw.) 0. Ktz. Fig. 57. 531] MELIOLA IN PORTO RICO — STEVENS 63 Florida Adentro, July 1, '15, 7653. Las Marias, July 10, '15, 8179. Mayaguez, June 24, 15, 7395. Rio Arecibo, K. 64.7, July 8, '15, 7791. Rio Tanama, July 6, '15, 7838. On Meihomia adscendens (Sw.) Kuntz. El Alto de la Bandera, July 15, '15, 8531, 8648. On Meibomia supina (Sw.) Britt. Cataiio, Dec. 3, '13, 4532. Martin Pefia, Aug. 11, '15, 9309. Mari- cao, July 19, '15, 8793, July 20, '15, 8975. Vega Baja, July 2, '15, 7750. Mayaguez, July 3, '15, 8094. Florida Adentro, July 15, '15, 7666. Rio Tanama, July 6, '15, 7854. Dos Bocas, below Utuado, July 8, '15, 8022. El Gigante, near Adjuntas, Dec. 15, '13, 5820. Indiera Fria, Oct. 8, '13, 3370. On Dalhergia monetaria L. fil. Mayaguez Mesa, June 25, '15, 7476. Arecibo-Lares Road, June 21, '15, 7243. Maricao, Sept. 20, '13, 3658. On Dalhergia sps. Mayaguez Mesa, June 29, '15, 7577. Rosario, Apr. 3, '13, 711, July 4, '15, 9016. On Bradhurya virginiana O. Ktz. Arecibo-Lares Road, June 21, '15, 7242. Manati, July 2, '15, 7694. Dos Bocas, below Utuado, July 8, '15, 8043. Vega Baja, July 2, '15, 7749. Quebradillas, Nov. 22, 13, 5036. On Mimosa ceratonia L. Maricao, July 20, '15, 8899, 8868. Rio Arecibo, K. 64.7, July 8, '15, 7770. Vega Baja, July 2, '15, 7744. Aibonito, Nov. 3, '13, 4020. On Lonchocarpus glaucifolius Urb. Quebradillas, June 20, '15, 7264. On Erythfina micropteryx Poepp. El Miradero, Aug. 11, '15, 9166. On Teramnus uncinatus (L.) Sw. Maricao, 3503, Jan. 10, '12, 228. Anasco, Oct. 12, '13, 3583, 3537. Yau^o, Oct. 3, '13, 3136. The form on Teramnus, Nos. 6554, 3583, 3503, is much branched at the apices. On Dolicholos reticulatus Millsp. Florida Adentro, July 1, '15, 7682, 70(75. Vega Baja, Nov. 5, '13, 4263. Lares, Nov. 22, 13, 4933. Quebradillas, Nov. 22, 13, 4982. Bar- celoneta, Aug. 10, '15, 9259. Rio Tanama, Aug. 6, '15, 7875. On an unknown Legume by Heller, 6259. This series of specimens on the various hosts shows considerable variation, particularly as to setal apices. The mycelium and the capi- tate hyphopodia, however, are quite characteristic and I am therefore 64 ILLINOIS BIOLOGICAL MONOGRAPHS [532 inclined to include all of these forms, growing as they do upon members of one family, in one single species, recognizing as varieties such as show well-marked and constant difference. These differences are usually in the setae, which on some hosts are almost invariably simple, on others slightly toothed, and on still others much toothed and contorted. The species as manifest on some of its hosts clearly agrees with M. bicornis Wint of Rabenhorst's Fungi Europaei, No. 3545, collected on an unknown legume in Brazil, May 1885 ; with an authentic specimen kindly loaned from the Kew Gardens ; with a specimen of the Heller col- lection determined by Earle and with a cotype specimen kindly sent to me by Dr. H. Sydow. It does not, however, conform to the description of this species as given by Gaillard, or the original description of Winter, in that the capitate hyphopodia are not often opposite, as they describe, and as Gaillard figures them, nor are the setae deeply bifid as he describes and figures. Since the original description was by Winter and the Brazilian specimen was published by Winter, and our specimen is clearly co-specific with the Brazilian specimen, it seems best to regard the pres- ent series as M. bicornis. On Meibomia, the tips are either acute and simple, or bifid or toothed. Some collections are uniformly simple, others uniformly toothed, yet the species appears to be identical. The collections on M. adscendens and M. axillaris are rarely forked, while those on M. supina are rarely undivided. One collection on M. axillaris, No. 7838, how- ever showed a remarkable variant, with much and very irregularly forked apices. The form on Erythrina has few setae and these sometimes contorted at the tip. The specimens on Bradbury a show the capitate hyphopodia somewhat more angular, with simple and unusally bent setae. 95a. M. BICORNIS var. calopogonu var. nov. On Calopogonium orthocarpum Urb. Dos Bocas, below Utuado, July 8, '15, 8060, Dec. 16, '13, 6035. Mayaguez, Oct. 31, '13, 3492, Apr. 10, '13, 372. Aguada, Nov. 22, '13, 5087. The form on Calopogonium shows perhaps greater differentiation than any of the other forms. The setae are more rigid, more uniformly fine toothed and the capitate hyphopodia are a trifle larger than on other hosts. 533] MELIOLA IN PORTO RICO — STEVENS 65 95b. M. BicOBNis VAB. GALACTiAE var. nov. On Oalactia duhia P. DC. Rio Tanama, July 6, 15, 7856 (type). The form on Galactia is well differentiated with large, capitate hyphopodia and usually with divided setae. / Meliola sp. indet. Mayaguez, May 1, '13, No. 1067 on a legume said by Percy Wilson to be "possibly a narrow leaved form of Clitoria ruhiginosa Juss." This collection consisting of a few leaves I have not named because of the scanty material. It is a 4-septate form with no mycelial setae, but with obtuse perithecial appendages which are curved, 27-51 x 7/i, Perithecia 125-172/i in diameter. Mycelium quite typical in the manner in which the threads anastomose. Capitate hyphopodia alternate, head cell nearly globular. Mucronate hyphopodia opposite or alternate, neck long, narrow. Spores 4-septate, 41 x 17/i, obtuse. M. cookenana, Speg. Specimens on Lippia, Stachytarpheta and Lantana, bearing this name are in the New York Garden collections. The determination on Lantana is evidently erroneous. The other speci- mens have not been examined. 66 ILLINOIS BIOLOGICAL MONOGRAPHS [534 HOST LIST Arranged by Families Polypodiaceae Adiantum latifolium Adiantum sp. Sehizaeaceae Aneimia adiantifolia Aneimia sp. Gramineae Andropogon bicomis AndropogoD leucostachyns Paspalum schreberianum Paspalum gliitinosum Panicum glutinosum Lasiacis swartziana Lasiacis compacta Lasiacis divaricata Ichnanthus pallens Oplisinenus setarius Olyra latifolia Stenotaphrum secundatum Chloris petraea M. pteridicola M. pteridicola M. pteridicola M. pteridicola M. panici M. panici M. panici M. panici M. panici M. panici M. panici M, panici M. panici M. panici M. panici M .panici M. stenotaphri M. panici Six other species have been reported upon Graminae as follows: M. bambusae, M. herculeus, M. arundinis, M. amphitricha, M. furcata, M. substenospora. Cyperaceae Mariscus jamaieensis M. cyperi Mariscus jamaieensis M. circinans Rhynchospora aurea M. circinans Cyperus sp. M. cyperi Scleria sp. M. C3T)eri Those previously reported upon the Cyperaceae are : M. circinans, M. intricata, M. amphitricha, M. argentina, M. uleana, M. cyperi-uleana, and M. cyperi-italica. Palmae Macrodiscus lactiflorus M. furcata Acrista monticola M. furcata Thrinax ponceana M. furcata Thrinax praeceps M. furcata Coccothrinax alta M. furcata 535] MELIOLA IN PORTO RICO — STEVENS 67 Roystonea borinquena M. denticulata Previously reported on the Palmae: M. iquitosensis, M. manaosen- sis, M, palmieola, M. amphitricha, M. contigua, M. furcata, M. hyalospora. Araceae Philodendron krebsii M. philodendri Dieffenbachia seguine M. dieffenbaehiae Liliaceae Srailax coriacea M. smilacis Three other species are reported on the Liliaceae, viz., M. hyalo- spora, M. subdentata, M. dracaenicola. Piperaceae Piper medium M, tortuosa Piper adunctum M, glabroides, M. piperis, and M, gaillardiana Piper peltatum M. tortuosa Piper umbellatum M. tortuosa Piper hispidum M. contorta Piper blattarum M. paucipes The species previously known upon Piper are: M. stenospora, M. asterinoides, M. patouillardi, M. pululahuensis, M, furcata, M. tortuosa, M. piperis, and M. glabra. Myricaceae Myrica cerifera M. manca Urticaceae Pilea parietaria M. earlii and M. triloba Pilea nummularifolia M. earlii Pilea sp. M. earlii M. thomasiana has also been reported upon the Urticaceae. Polygonaceae Coccolobis pyrifolia M. praetervisa Coecolobis sentenisii M. praetervisa Coccolobis laurifolia M. rectangularis Coccolobis sps. M. praetervisa Magnoliaceae Magnolia portoricensis M. magnoliae M. amphitricha and M. piptochaeta have previously been reported upon this family. Anonaceae Anona montana M, longipoda M. uvariae has been reported upon this family. Lauraceae Persea gratissima M. perseae 68 ILLINOIS BIOLOGICAL MONOGRAPHS [536 Ocotea leucoxylon ' M. ocoteae and M. ocoteicola Nectandra patens '' M. glabroides The following species have heretofore been reported upon the Lau- raceae : M. calva, M. ziz-zag, M. praetervisa, M. martiniana, M. penicil- lata, M. anomala, M, manea, M. an aphitricha, M. acutiseta. Rosaceae Rubus sp. M, puiggarii Leguminosae Inga laurina M. toruloidea Mimosa ceratonia M. bicornis Lonehocarpus glaucifolius M. bicornis Cassia quinquadrangulata M. toruloidea Clitoria rubiginosa ( ?) M. sp. ind. • Meibomia axillaris M. bicornis Meibomia adscendens M. bicornis Meibomia supina M. bicornis Dalbergia monetaria M. bicornis Dalbergia sps. M. bicornis Andira jamaicensis M. andirae Bradburya virginiana M. bicornis Erythrina micropteryx M. bicornis » Teramnus uncinatus M. bicornis Rudolphia volubilis M. rudolphiae Calopogonium orthocarpum M. bicornis var. ealopogonii Galaetia dubia M. bicornis var. galactiae Dolicholus reticulatus M. bicornis Chamaecrista granulata M. chamaecristicola Chamaecrista ' ' glandulosa ' ' M. chamaeeristae The following species of Meliola have been reported upon the Legu minosae: M. desmodii, M. ludibunda, M. malacotricha, M. bicornis, M. juruana, M. chamaeeristae, M. denticulata, M, harioti, M. pellucida, M. zollingeri, M, andirae, M. gleditschiae, M. musae, M. pazschkeana, M. pseudoanastomosans, M. subtorulosa, M. tamarindi. Rutaceae Pilocarpus racemosus M, pilocarpi Amyris elemifera M. monensis In addition to the species mentioned above, the following have been reported upon the Rutuceae: M. obesa, M. evodiae, M. obesula, M. tenella, M. microtheca, M. butleri, M. amphitricha, M. patens, M. ludi- bunda. 537] MELIOLA IN PORTO RICO — STEVENS 69 Simarubaceae Simaruba tulae M. glabroides Meliaceae Guarea trichilioides M. guareae and M. giiareicola Other species described upon the Meliaceae are : M, macalpini, M. sandarici, M. opposita, M. parvula. Malpighiaceae Banisteria laurifolia M. rectangularis Byrsonima lucida M. byrsonimae Two other species have been reported upon the Malpighiaceae, namely, M. stuhlmanniana and M. crenata. Euphorbiaceae Drypetes sps. M. glabra Acalypha bisetosa M. arecibensis Jatropha hernandifolia M. jatrophae Gymnanthes lucida M. gymnanthicola Other forms reported upon the Euphorbiaceae are: M. malacotri- cha, M. patella, M. verrucosa, M. acalyphae, M. manihoticola, M. comu- caprae. Anacardiaceae Mangifera indica M. mangiferae Spondias mombin M. comocladiae Comocladia glabra M. comocladiae The following species have been reported upon the Anacardiaceae : M. rhois, M. guercinopsis, M. malacotricha, M. lanigera, M. irradians, M. mangiferae, M. polytricha, M, anacardii, M. genieulata, M. coronata, M. dracontomeli. Aquifoliaceae Ilex nitida M. raaricaensis Other species reported upon the Aquefoliaceae are: M. ilicis, M. lagersheiraii, M. cornuta, M. yerbae. Celastraceae Crossopetalum pallens M. compacta Species previously reported upon the Celastraceae are : M, falcati- seta and M. gymnosporiae. Staphylaceae : Turpinia panniculata M, guignardi Sapindaceae Serjania polyphylla M. serjaniae PauUinia pinnata M. hessii and M. pauUiniae Thouinia striata M. thouiniae 70 ILLINOIS BIOLOGICAL MONOGRAPtIS [538 Allophylus crassinervis M. thouiniae Cupania araericana M. cupaniae and M. praetervisa Cupania sps. M. cupaniae Hypelate trifoliate M. glabra Following is a list of the species previously reported upon the Sapin- daceae: M. wrightii, M, parenchymata, M. sapindacearum, M. cruci- fera, M. ambigua, M. thouiniae, M. araneosa, M. bombphlandi. Rhamnaceae Krugiodendron ferreum M. thouineae Gouania lupuloides M. tenuissima Previously reported upon the Rhamnaceae, M. scutiae. Vitaceae Cissus sicyoides M, merrillii One other species is reported upon the Vitaceae, namely, M. paracusis. Tiliaceae Triumfetta semitriloba M. triumfettae Malvaceae Sida urens M. molleriana • Hibiscus tiliaeeus M. triumfettae Two species have been reported upon the Malvaceae, viz., M. mol- leriana and M. siflae. Ochnaceae Sauvagesia erecta Guttiferae Calophyllum calaba Mammea americana Clusia minor Winteranaceae Winterana canella Flacourtiaceae Casearia ramiflora Casearia sylvestris Casearia arborea Casearia aculeata Casearia sp. Cambretaceae Laguncularia racemosa M. glabroides iM. calophylli M. pauUiniae M. clusiae M. thouiniae M. pauUini'ie M. pauUiniae M. pauUiniae M. pauUiniae M. pauUiniae M. lagunculariae and M. nigra Only one species had heretofore been reported upon this family. Myrtaceae Amomis caryophyllata M. amomicola Psidium guajava M. psidii 539] MELIOLA IM PORTO RICO — STEVENS 71 Myrcia deflexa M. helleri Myrcia splendens M. helleri Eugenia stahlii M. helleri Eugenia monticola M. helleri The following have been recorded upon the Myrtaceae : M. psidii, M. helleri, M, puchella, M, arborescens, M. laxa, M. densa, M. horrida, M. cladotricha, M. elavispora, M, valdivivensis. Melastomataceae Mieonia laevigata M. melastomacearum Miconia racemosa M. melastomacearum Mieonia sentenisii M. mieonieicola Miconia prasina M. miconiae Clidemia hirta M. melastomacearum Clidemia strigillosa M. melastomacearum Species other than the above which have been reported upon the Melastomataceae are : M. heudeloti, M. weigettii, M. memecyli, M. affinis. Araliaceae Dendropanax arboreum M. didymopanicis Dendropanax laurifolium M. didymopanicis Other species on the Araliaceae are : M. araliae, M. dichotoma, M. kusanoi, M, heterosetae, M. pectinata, M. leplidae. Myrsinaceae Ardisia guadalupensis M. myrsiniacearum Parathesis serrulata M. parathesicola The following species have been reported upon the Myrsinaceae: M. cladotricha, M. quercinopsis, M. delicatula, M. armata, M. groteana, M. perigrina, M. maesae. Sapotaceae Lucuma multiflora M. lucumae Dipholis salicifolia M. dipholidis Chrysophyllum sp. M. ocoteicola M. brasiliensis is the species previously reported on the Sapotaceae. Oleaceae M. raayepeae Mayepea domingensis M. mayepeicola M, jasminicola has been reported on this family. Apocynaceae Plumiera krugii M. tabernaemontanae Tabernaemontana oppositifolia M. tabernaemontanae Rauwolfia nitida M. tabernaemontanae Forsteronia corymbosa M. tabernaemontanae var. for- steroniae 72 ILLINOIS BIOLOGICAL MONOGRAPHS [540 Other species previously recorded upon the Apocynaceae are: M. levipoda, ]\I. simillima, M. intermedia, M. willoughbyae, M. clavitispora, M. compositarum, M. membranacea, M. laevigata. Convolvulaceae Ipomoea cathartica M. clavulata, M. ipomoeae, and M. quadrispina Ipomoea tiliacea M. clavulata and M. ipomoeae Ipomoea batatas M. clavulata Ipomoea sps. M. clavulata and M. ipomoeae Other species recorded for this family are: M. decidua, M. ambi- gua, M. melacotricha, M. ipomoeaephile, M. merremiae, M. franceville- ana, M. caymanensis, M. hewittiae. Borraginaceae Cordia nitida M. longipoda Cordia sps. M. longipoda Tournefortia hirsutissima M. longipoda Varronia sps. M. molleriana In addition to the above, M. usteriana has been reported upon the Borraginaceae. Verbenaceae Stachytarpheta cayennensis M. glabroides Lantana odorata M. ambigua. Lantana camara M. ambigua Lantana sps. M. ambigua Lantana sps, M. cookeana (?) Avicennia nitida M. sepulta Other species recorded on the Verbenaceae are: M. durantae, M. sakawensis, M, caUicarpae, M. clerodendricola, M. amphitricha, M. lip- piae, M. coronata, M. vitis, M. lantanae. Labiatae Hyptis lantanifolia M. hj-ptidicola H3T)tis capitata M. hyptidicola Hyptis pectinata M. hyptidicola Hyptis sp. M. hyptidicola Other species recorded on the Labiateae are: M. anastomosans, M. inermis, M. pelliculosa, M. hyptidis. Solanaceae Solanum rugosum M. glabroides Solanum persicifolium M. glabroides Solanum jamaicense M. solani Capsicum baccatum M. capsicola Oestrum laurifolium M. gesneriae Oestrum macrophyllum M. gesneriae 541] MELIOLA IN PORTO RICO — STEVENS 73 Other species on the Solanaeeae are: M. winteri, M. plebeja, M. glabra, M. solanicola Gaill., M. acervata, M. solanieola P. Henn. Bignoniaceae Macrodiscus lactiflonis M, furcata Tecoma pentaphylla M. tecomae and M. bidentata Tecoma sps. M. tecomae Tabebuia haemantha M. bidentata Schlegelia sps. M. glabroides var. schlegeliae Species previously recorded upon the Bignoniaceae are: M. arachnoidea, M. bidentata, M. furcata, M. harioti, M. lanceolato-setosa. Gesneriaceae Gesneria albiflora Acanthaceae Hygrophila brasiliensis Rubiaceae Gonzalagunia spicata Randia aculeata Erithalis fruticosa Chiococca alba Guettarda ovalifolia Palicourea crocea Palicourea riparia Palicourea domingensis Palicourea sps. Borreria laevis Borreria ocimoides Coccocypselum repens Psychotria pubescens Psychotria grandis Psychotria bertiana Psychotria sp. Rubiaceae indet. Other species on the Rubiaceae are : M. intermedia, M. sandicensis, M. longiseta, M. falcata, M. manca, M. rubicola, M. cryptocarpa, M. gla- bra, M. palawanensis, M. pencilliformis, M. asterinoides, M. mitchelliae. Cucurbitaceae Cayaponia (?) M. cucurbitaeearum Three species, none of them with forked setae, have been noted upon the Cucurbitaceae, namely : M. triloba, M. malacotricha, M, aciculosa. M. gesneriae M. irregularis M. psychotriae M. psychotriae M. psychotriae M. chiococcae and M. psychotriae M. psychotriae M. mayaguesiana M. mayaguesiana M. mayaguesiana and M. glabra var. psychotriae M. mayaguesiana and M, glabra var. psychotriae M. psychotriae M. psychotriae M. glabra var. psychotriae M. glabra var. psychotriae M. glabra var. psychotriae M. glabra var. psychotriae M. glabra var. psychotriae M. psychotriae 74 ILLINOIS BIOLOGICAL MONOGRAPHS [542 Compositae Pseudelephantopus spicatus M. cyclopoda Eupatorium odoratum M. compositarum Eupatorium portoricense M. compositaruin var. portori- censis Species previously reported upon the Compositae are : M. sororcula, M. spegazziniana, M. mikaniae, M. compositarum, M. inermia, M. am- phitricha, M. tortuosa. Host unknown M. bidentata Host unknown M. amphitriciia Host unknown M. tuberculata Host unknown M. paulliniae Host unknown M. aibonitensis Host unknown M. sp. ind. 543] MELIOLA IN PORTO RICO — STEVENS 75 EXPLANATION OF PLATES All plates are from photo-micrographs made by Mr. A. G. Eldredge. They were reduced 28% in reproduction. The abbreviations l.p. and h.p. indicate low power and high power with a magnification of ap- proximately 75 and 355 diameters respectively. Scales showing the magnification are given in plate V ; the smallest divisions are 10/a wide. 76 ILLINOIS BIOLOGICAL MONOGRAPHS [544 EXPLANATION OF PLATE I Fig. I. M. puigiarii : portion of perithecium and larvaeform appendages, h.p. No. 8270. Fig. 2. M. manca : mycelium, capitate hyphopodia, a germinating spore, h.p. No. 1292 N. A. F. Fig. 3. M. guignardi : general type of colony, mycelium and capitate hyphopodia. No. 8922. l.p. Fig. 4. M. glabra : showing type of mycelium and hyphopodia. l.p. Rabenhorst, Fung. Europ. Xo. 3849. Fig. 5. M. sepulta : type of colony and mycelium, l.p. Heller's collection. No. 6416. Fig. 6. M. irregularis : spores showing irregularity in size of cells, h.p. No. 9283 (type). Fig. 7. M. solani: mycelium showing opposite hyphopodia. h.p. No. 5750 (type). Fig. 8. M. hyptidicola : a showing perithecia, crooked mycelium, and oval hypho- podia. l.p. b mycelium and hyphopodia in greater detail, h.p. No. 8526. Fig. 9. M. cyclopoda : general view showing colony, rough perithecia, and char- acter of mycelium and hyphopodia. l.p. No. 7733 (type). Fig. 10. M. perseae : irregular, crooked mycelium, mucronate hj-phopodia and an- gular capitate hyphopodia. h.p. No. 8212 (tjrpe). Fig. II. M. longipoda: mycelium, capitate and mucronate hyphopodia, ascospores and a young perithecium. h.p. No. 9329. 545] MELIOLA IN PORTO RICO-STEVENS 77 PLATE II 78 ILLINOIS BIOLOGICAL MONOGRAPHS [546 EXPLANATION OF PLATE II Fig. 12. M. melastomacearum : perithecia, mycelium and hyphopodia. l.p. No. 8956. Fig. 13. M. glabroides : germinating spore with hyphopodia. h.p. No. 3371 (type). Fig. 14. M. triloba : spore and young mycelium with typically lobed capitate hy- phopodia. h.p. No. 1038. Fig. 15. M. compositarum : colony showing perithecium wit^ larvaeform append- age, mycelium and hyphopodia. l.p. Heller, No. 6185 (co-type). Fig. 16. M. compositarum var. portoricensis : showing mycelium and capitate hy- phopodia. h.p. No. 7723. Fig. 17. M. calophylli: irregular mycelium and head cells, h.p. No. 7059 (type). Fig. 18. M. arecibensis : mycelium and crowded hyphopodia. h.p. No. 365a (type). Fig. 19. M. miconieicola : loose, scant mycelium with distant hyphopodia. l.p. No. 8639 (type). Fig. 20. M. parathesicola : capitate hyphopodia very crowded, h.p. No. 7286. Fig. 21. M. toruloidea : perithecia, young and old, mycelium and hyphopodia. l.p. No. 8394 (type). A few setae are visible on the perithecia. Fig. 22. M. comocladiae : colony showing character of mycelium and hyphopodia. l.p. No. 9015 (type). 547] MELIOLA IN PORTO RICO — STEVENS 79 PLAVE III 80 ILLINOIS BIOLOGICAL MONOGRAPHS [548 EXPLANATION OF PLATE III Fig. 23. M. tortuosa : perithecium and appendages, with tortuose tips. l.p. No. 4714. Fig. 24. M. chamaecristicola : showing thin setae on perithecia. l.p. No. 61 13 (type). Fig. 25. M. rectangularis : showing right-angle type of branching, h.p. No. 7292 (type). Fig. 26. M. chiococcae : mycelium, angular hyphopodia, young perithecium with radiating mycelium, h.p. No. 7743 (type). Fig. 2J. M. pteridicola : showing parallel coursing of mycelium, l.p. No. 7814 (type). Fig. 28. M. cupaniap : showing perithecia! setae, l.p. No. 9143 (type). Fig. 29. M. miconiae : general habit of mycelium, l.p. No. 9366. Fog. 30. M. contorta : perithecia and crooked perithecial setae, l.p. No. 8225 (type). Fig. 31. M. mayaguesiana : ascospore, mycelium and capitate hyphopodia. h.p. No. 7157 (type). Fig. ^2. M. clavulata: swollen setal tips. h.p. No. 7837. Fig- Zi- ^I- praetervisa : spore and mycelium showing characteristic conic capitate hyphopodia. h.p. No. 5653a. ;:,-*, «-«JU T'' dOQES. J- ?xi.' 549] MELIOLA IN PORTO RICO — STEVENS 81 PLATE IV 82 ILLINOIS BIOLOGICAL MONOGRAPHS [550 EXPLANATION OF PLATE IV Fig. 34. M. andirae : a spore, mycelium showing characteristic opposite capitate hyphopodia. h.p. Earle (co-type). No. 6448. Fig. 35. M. monensis : showing typical hyphopodia. h.p. No. 6158 (type). Fig. 36. M. thouiniae : capitate and mucronate hyphopodia. h.p. Heller, l\o. 6435 (type). Fig. 37. M. amomicola: typical opposite hyphopodia. h.p. No. 7054 (type). Fig. 38. M. stenotaphri : crooked mycelium, long setae, l.p. No. 8023. Fig. 39. M. capsicola : showing irregular hyphopodia. l.p. No. 8019 (type). Fig. 40. M. paucipes : distant hyphopodia and long, black setae, l.p. No. 7463 (type>. Fig. 41. M. rudolphiae: mycelium, capitate hyphopodia and a spore, h.p. No. 8698. Fig. 42. M. serjaniae : alternate capitate and opposite mucronate hyphopodia. h.p. No. 425 (type). Fig. 43. M. ocoteicola : characteristic capitate hyphopodia. h.p. No. 7560 (type). Fig. 44. M. polytricha K. & C. : edge of colony showing habit. "No. 1262 on Cunonia capensis. Grahamstown, Cape, ex Kalchbrenner." h.p. (type). \ ^ z:^ 551] MELIOLA IN PORTO RICO — STEVENS 83 PLATE V 84 ILLINOIS BIOLOGICAL MONOGRAPHS [552 EXPLANATION OF PLATE V Fig. 45. M. earlii: colony showing mycelial character, l.p. No. 7685 (type). Fig. 46. M. mayepeae : general character of colony, l.p. No. 7468 (type). Fig. 47. M. lucumae : mycelium and capitate hyphopodia, young perithecium. l.p. No. 8164 (type). Fig. 48. M. tecomae: showing curved tips of setae, h.p. No. 9332 (type). Fig. 49. M. ipomoeae : colony, mycelium, opposite hyphopodia, long setae, peri- thecia. l.p. No. 6563. Fig. 50. M. magnoliae : seta! tip showing forking, h.p. No. 4738 (type). Fig. 51. M. cucurbitacearum : showing forking of setal tips. h.p. No. 8732 (type). Fig. 52. M. hessii : a setum showing character of forking, a bit of mycelium, l.p. No. 9367 (type). Fig. 53. M. quadris'pina : general type of colony, l.p. No. 8163. Fig. 54. M. philodendri : primary branching of setal tip. h.p. No. 4346. Fig. 55. M. gaillardiana : crested setum. h.p. No. 7794 (type). Fig. 56. M. dieffenbachiae : setal tip. h.p. No. 8148 (type). Fig. 57. M. bicornis, "authentic specimen" from Kew gardens determined by Winter : spore, mycelium and capitate and mucronate hyphopodia. h.p. Fig. h.p. millimeter scale ruled to i/io mm. high power. Fig. l.p. ditto with low power magnification. ^ 1 553] MELIOLA IN PORTO RICO — STEVENS 85 ALPHABETICAL LIST OF SPECIES Meliola aibonitensis, i6 ambigua, 50 amomicola, 40 amphitricha, 49 andirae, 38 araliae, 40 arecibensis, 23 bicornis, 62 bicornis var. calopogonii, 64 bicornis var. galactiae, 65 bidentata, 62 byrsonimae, 49 calophylli, 22 capsicola, 41 chamaecristae, 47 chamaecristicola, 26 chiococcae, 27 circinans, 33 clavulata, 37 clusiae, 52 comocladiae, 25 compacta, 30 compositarum, 21 compositarum var. portoricensis, 22. contorta, 32 cookeana, 65 Meliola cucurbitacearum, 58 cupaniae, 29 cyclopoda, 16 cyperi, 34 denticulata, 57 didymopanicis, 39 dieffenbachiae, 62 dipholidis, 44 earlii, 47 furcata, 58 gaillardiana, 61 gesneriae, 47 glabra, 13 glabra var. psychotriae, 14 glabroides, 18 glabroides var. schlegeliae, 20 guareae, 55 guareicola, 53 guignardi, 12 gymnanthicola, 49 helleri, 56 hessii, 59 hyptidicola, j6 86 INDEX [554 Meliola ipomoeae, 54 irregularis, 15 jatrophae, 48 lagunculariae, 17 longipoda, 17 lucumae, 49 magnoliae, 55 manca, 11 mangi ferae, 57 maricaensis, 31 mayaguesiana, 2^ mayepeae, 48 mayepeicola, 51 melastomacearum, 18 merrillii, 61 miconiae, 30 miconieicola, 2^ moUeriana, 29 monensis, 38 myrsinacearum' 40 nigra, 27 octoeae, 29 ocoteicola, 45 panici, 42 parethesicola, 24 paucipes, 42 Meliola paulliniae, 45 philodendri, 60 pilocarpi, 41 piperis, 59 perseae, 17 praetervisa, 38 psidii, 36 psychotriae, 51 pteridicola, 28 puiggarii, 11 quadrispina, 60 rectangularis, 2y rudolphiae, 43 sepulta, 14 serjaniae, 44 smilacis, 56 solani, 15 sp. indet., 65 stenotaphri, 41 tabernaemontanae, 50 tabernaemontanae var. f orsteroniae, 50 tecomae, 53 tenuissima, 24 thouiniae, 39 tortuosa, 26 toruloidae, 25 triloba, 20 triumfettae, 30 tuberculata, 23 \*> ■■}>'">''"^*-X' ■4^/''^<^'^' kisx^^