bigram

This is a table of type bigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

bigram	frequency
porcine epidemic	247
epidemic diarrhea	215
diarrhea virus	181
time pcr	163
respiratory syndrome	125
porcine reproductive	106
clinical signs	99
syndrome virus	96
serum samples	95
time rt	90
present study	89
porcine circovirus	83
canine parvovirus	80
clostridium difficile	79
vaccine strains	78
escherichia coli	70
united states	67
additional file	66
time rpa	66
attenuated vaccine	64
healthcare professionals	61
doc id	60
vet res	60
cord uid	60
fecal samples	60
res doi	60
bmc vet	60
control group	59
immune response	57
circovirus type	57
pcr assay	57
rpa assay	56
risk factors	55
giardia spp	55
respiratory disease	55
new ica	55
transmissible gastroenteritis	54
authors declare	53
mg ml	51
positive control	50
virus infection	50
infectious diseases	49
canine distemper	49
competing interests	48
pedv strains	48
common goal	48
infectious bronchitis	47
tt arac	46
nucleic acid	46
bovine coronavirus	45
reverse transcription	44
bovine respiratory	44
classical attenuated	43
current study	43
significantly higher	43
final manuscript	42
performed using	42
indirect elisa	42
antiviral activity	41
avian influenza	41
infected pigs	41
polymerase chain	41
animal health	41
negative control	41
polymerase amplification	40
distemper virus	40
recombinase polymerase	40
cryptosporidium spp	40
chain reaction	40
influenza virus	39
salmonella spp	39
phylogenetic analysis	38
diarrhoeic piglets	38
vaccine strain	38
clinical samples	37
swine sera	37
rapid detection	37
control pigs	37
veterinary medicine	36
gastroenteritis virus	36
one health	36
salmonella enterica	36
bronchitis virus	35
feline coronavirus	35
epidemic diarrhoea	34
authors read	34
pcv infection	34
economic losses	34
significant differences	34
knowledge sharing	33
nucleotide sequences	33
commercial meat	33
clostridium perfringens	33
statistical analysis	33
previously described	33
linked immunosorbent	32
diarrheic dogs	31
significant difference	31
bad fur	31
viral rna	31
fecal microbiota	31
pig farms	31
strain rsc	30
previous studies	30
two groups	30
treatment group	30
immunosorbent assay	30
amino acid	30
animal species	30
primary pams	30
immune responses	30
plant extracts	30
pcr assays	30
ibv infection	29
herd size	29
rpa assays	29
viral dna	29
neonatal diarrhoea	29
water buffalo	29
spike protein	29
isothermal amplification	28
feline panleukopenia	28
pcr positive	28
respiratory syncytial	28
prrsv infection	28
positive samples	28
veterinary healthcare	28
sirs criteria	27
syncytial virus	27
diarrhoea virus	27
statistically significant	26
prrsv rjxwn	26
samples collected	26
rna viruses	26
infected animals	25
institutional affiliations	25
porcine respiratory	25
remains neutral	25
jurisdictional claims	25
gene expression	25
next manuscript	25
previous study	25
springer nature	25
nature remains	25
biomed central	25
infected cells	25
swine industry	25
immunodeficiency virus	25
data collection	25
agarose gel	25
published maps	25
feline immunodeficiency	24
animal care	24
blood samples	24
mutual differentiation	24
faecal samples	24
results showed	24
genome sequence	23
health status	23
panleukopenia virus	23
ng ml	23
quality control	23
organ dysfunction	23
chicken shandong	23
host response	23
cell culture	23
acute respiratory	23
complete genome	23
rpa method	23
molecular characterization	23
mouth disease	23
rna virus	23
highly pathogenic	22
genetic diversity	22
rectal swabs	22
direct contact	22
common ingroup	22
nitric oxide	22
swine influenza	22
balf samples	22
choleraesuis vaccine	22
sequence analysis	22
professional visitors	22
even though	22
south korea	22
regulated delayed	22
previous reports	21
viral load	21
sample collection	21
ingroup identity	21
poultry trade	21
nucleic acids	21
fur mutation	21
typhimurium strains	21
closely related	21
enteropathogenic species	21
recombinant protein	21
three times	21
virus strains	21
pedv infection	21
study design	21
days post	21
analytical sensitivity	20
real time	20
neutralizing antibodies	20
canine coronavirus	20
vp gene	20
western blot	20
total number	20
based real	20
supplementary material	20
thai medicinal	20
gel electrophoresis	20
buffered saline	20
thai cakovs	20
oral fmt	20
domestic dogs	20
amino acids	20
pasteurella multocida	20
per reaction	20
small intestine	19
treatment groups	19
corresponding author	19
described previously	19
tcid ml	19
buffalo calves	19
type strains	19
serological assays	19
feed pellets	19
experimental infection	19
age group	19
disease control	19
veterinary medicines	19
applied biosystems	19
pedv rna	19
acute phase	19
lfs rpa	19
disease virus	19
healthy dogs	19
respocheck triplex	19
triplex pcr	19
respiratory coronavirus	18
tested positive	18
field strains	18
spike gene	18
neonatal diarrhea	18
cattle herds	18
conventional pcr	18
mediated isothermal	18
feline leukemia	18
vero cell	18
rebov infection	18
leukemia virus	18
meat rabbits	18
european countries	18
higher prevalence	18
epithelial cells	18
swine fever	18
performance herds	18
pedv detection	18
rrna gene	18
ornithobacterium rhinotracheale	18
neonatal piglets	18
results indicated	17
suckling piglets	17
pedv classical	17
highly conserved	17
severe acute	17
monoclonal antibodies	17
cfu ml	17
stool samples	17
multisystemic wasting	17
affected farms	17
control dogs	17
respiratory tract	17
goat anti	17
sequence data	17
live poultry	17
inflammatory response	17
mortality rate	17
reasonable request	17
high mortality	17
negative samples	17
high prevalence	16
disease complex	16
postweaning multisystemic	16
enteric disease	16
pcr analysis	16
calculated using	16
institutional animal	16
sequences obtained	16
copies per	16
psr assay	16
hoof temperature	16
identity model	16
dairy calves	16
well plates	16
rabbit fecal	16
fever virus	16
ort chicken	16
contaminated feed	16
room temperature	16
least one	16
complete feed	16
phylogenetic tree	16
strains isolated	16
enterica serovar	16
accession numbers	16
field pedvs	16
enteric pathogens	16
dairy cattle	16
bovine serum	16
accession number	16
mice immunized	16
pcr products	16
wasting syndrome	16
mycoplasma ovipneumoniae	16
experimentally infected	16
network analysis	16
clinical disease	16
use committee	16
positive serum	15
bovine herpesvirus	15
data analysis	15
differentially expressed	15
different types	15
study period	15
spatial scan	15
will help	15
tissue samples	15
high sensitivity	15
positive results	15
mycoplasma triplex	15
respocheck mycoplasma	15
antibody responses	15
disease spread	15
villous atrophy	15
determined using	15
test kit	15
swine stool	15
antimicrobial use	15
medicinal plant	15
alveolar macrophages	15
highly virulent	15
virus titer	15
nucleocapsid protein	15
serially diluted	15
nasal swabs	15
viral pathogens	15
animal welfare	15
north american	15
lateral flow	15
widely used	15
analyzed using	15
risk factor	15
statistical association	15
live attenuated	15
attenuated salmonella	15
feed material	14
antimicrobial susceptibility	14
viral infection	14
viral replication	14
chestnut tannins	14
disease status	14
ambient temperature	14
rescue dogs	14
fecal shedding	14
swine operation	14
previously reported	14
type i	14
see additional	14
significantly lower	14
statistical analyses	14
total rna	14
pcr results	14
mitochondrial apoptosis	14
ethics approval	14
naturally infected	14
parvovirus type	14
tritrichomonas foetus	14
sybr green	14
state university	14
virulence factors	14
viral titers	14
results suggest	14
strains rsc	14
pathological changes	14
pedv real	14
adult cattle	14
structural proteins	14
rice bran	14
serial dilutions	14
infectious agents	13
antiviral activities	13
whole genome	13
convenient online	13
felv infections	13
virus isolation	13
retail markets	13
standard dna	13
relative abundance	13
negative controls	13
pcr product	13
japanese strains	13
standard curve	13
space constraints	13
council regulation	13
mycoplasma species	13
specific antibodies	13
faecal sample	13
sera collected	13
triplex real	13
clinical specimens	13
take full	13
peer review	13
diarrhea viruses	13
lymph nodes	13
respiratory distress	13
faecal microbiota	13
pcv infected	13
prrsv replication	13
negative serum	13
avian infectious	13
associated diarrhoea	13
google scholar	13
thorough peer	13
freely available	13
open reading	13
realtime pcr	13
zna probe	13
sappan extract	13
also reported	13
diagnostic sensitivity	13
diarrhoeic cats	13
online submission	13
bird markets	13
immune system	13
animal populations	13
intestinal tract	13
full advantage	13
japanese field	13
united kingdom	13
small animal	13
peripheral blood	13
pcr dgge	13
virus neutralization	13
recent years	13
immediate publication	13
cape verde	13
health care	13
commercially available	12
dna extraction	12
molecular epidemiology	12
control measures	12
alpha toxin	12
generation sequencing	12
viral diarrhea	12
average daily	12
contact log	12
lymph node	12
old piglets	12
rpa primers	12
innate immune	12
reference intervals	12
enteropathogen co	12
lamina propria	12
us prrsv	12
borne infections	12
high morbidity	12
every step	12
highly contagious	12
weaned calves	12
conducted using	12
nonstructural protein	12
classical swine	12
host immune	12
age groups	12
test strip	12
significantly different	12
scan test	12
washed times	12
tested using	12
growing pigs	12
life technologies	12
conjunctival swabs	12
clinically healthy	12
graphpad prism	12
antibody titers	12
pedv negative	12
months old	12
type iii	12
ng well	12
indel strains	12
virus strain	12
electron microscopy	12
salmonella typhimurium	12
cdv pcr	12
ebola virus	12
day post	12
nc membrane	12
master mix	12
dna extracted	12
pedv positive	12
difficile infection	12
gold nanoparticles	12
statistical significance	12
respiratory symptoms	12
gut microbiota	12
reverse primers	12
genetic characterization	12
animal disease	11
genomic dna	11
ped outbreaks	11
sample pad	11
viral reads	11
logistic regression	11
laci tt	11
high risk	11
bad crp	11
live bird	11
canine kobuvirus	11
well plate	11
swine herds	11
dna copies	11
contains supplementary	11
idexx laboratories	11
weeks old	11
pbs group	11
antibody positive	11
conjugate pad	11
microbiota composition	11
molecular detection	11
different species	11
risk feed	11
absorbent pad	11
swab samples	11
associated disease	11
hx strain	11
infection status	11
full length	11
transcript abundance	11
veterinary diagnostic	11
specific iga	11
note springer	11
virus infections	11
nested pcr	11
serovar typhimurium	11
case report	11
standard plasmid	11
administrative regions	11
prrsv vr	11
diagnostic laboratory	11
virus detection	11
online version	11
chlamydia spp	11
pcv replication	11
rna extraction	11
ct values	11
domestic cats	11
blood agar	11
bovine viral	11
suckling pigs	11
glycosylation sites	11
prrsv strain	11
order nidovirales	11
parvovirus infection	11
agricultural university	11
raw food	11
evaluated using	11
viral shedding	11
cdv isolates	11
future studies	11
adult animals	11
causative agent	11
using real	11
north america	11
significant statistical	11
fusion protein	11
positive reaction	11
copy number	11
infected dogs	10
conserved region	10
viral infections	10
pedv antibody	10
tgev infection	10
large number	10
growth performance	10
protein microarray	10
first time	10
watery diarrhea	10
new neonatal	10
may also	10
pig farm	10
infected birds	10
wholesale markets	10
gene sequences	10
swine viruses	10
fecal score	10
modified live	10
positive dogs	10
prrsv infectivity	10
free water	10
monoclonal antibody	10
veterinary clinics	10
stranded rna	10
informed consent	10
feeding behavior	10
total phenolic	10
amplification assay	10
detected using	10
asymptomatic pigs	10
prrsv nsp	10
infected cats	10
may play	10
within min	10
disease outbreak	10
mucosal iga	10
rectal swab	10
type strain	10
protective immune	10
geographical distribution	10
poor performance	10
perfringens alpha	10
good performance	10
northern vietnam	10
time points	10
pedv strain	10
contaminated complete	10
spread rapidly	10
ibv nsp	10
babesia spp	10
subunit vaccine	10
mortality rates	10
outcome prediction	10
swine serum	10
nonstructural proteins	10
body temperature	10
healthy cats	10
sangon biotech	10
immune status	10
delayed fur	10
analytical specificity	10
protective immunity	10
porcine alveolar	10
toxin genes	10
pigs infected	10
wrote paper	10
technical assistance	10
molecular evolutionary	10
srb calves	10
animal experiments	10
small intestinal	10
treated feed	10
clinical presentation	10
infected group	10
pcv dna	10
stray dogs	10
first moderate	10
simultaneous detection	10
food diets	10
important role	10
forward primer	10
nucleotide sequence	10
natural compounds	10
cryptosporidium parvum	10
difficile strains	10
ct value	10
copy numbers	10
parvovirus dna	10
daily gain	10
thank dr	10
domestic rabbits	10
gastroenteritis coronavirus	10
binding sites	10
time polymerase	10
molecular biology	9
quantitative real	9
sensitive detection	9
case number	9
pig herds	9
analysis showed	9
authors thank	9
infectious disease	9
serum amyloid	9
total piro	9
purified recombinant	9
coronavirus infection	9
per day	9
results obtained	9
porcine transmissible	9
indirect contacts	9
many countries	9
neutralization test	9
neutralizing antibody	9
cell surface	9
infected communes	9
public health	9
urinary tract	9
propionibacterium acnes	9
phylogenetic analyses	9
physical examination	9
porcine ifn	9
differential expression	9
commercial rabbits	9
healthy pigs	9
positive result	9
reverse transcriptase	9
milk replacer	9
na pedv	9
laboratory rabbits	9
genetic variation	9
green i	9
primers used	9
routine antimicrobial	9
calves affected	9
large numbers	9
targeting nsp	9
intestinal lesions	9
disease prevention	9
administrative region	9
authorized users	9
purified using	9
orf gene	9
phylogenetic trees	9
vaccine real	9
pedigree cats	9
th percentile	9
plasmid dna	9
liquid antimicrobial	9
proportional morbidity	9
respiratory rate	9
gold standard	9
fold serially	9
wild carnivores	9
virus belonging	9
pedv vaccine	9
streptococcus suis	9
renal dysfunction	9
bootstrap values	9
reference strains	9
natural feeding	9
mucosal immune	9
quantitative pcr	9
prrsv titers	9
companion rabbits	9
fecal sample	9
pcv strain	9
case pigs	9
crt fusion	9
raw meat	9
porcine kobuvirus	9
sera showed	9
psr method	9
high specificity	9
detection method	9
evolutionary genetics	9
idexx ibv	9
also tested	9
electronic supplementary	9
genetics analysis	9
feed samples	9
across europe	9
relative abundances	9
capsid protein	9
strain cv	9
rectal temperature	9
samples using	9
live vaccines	9
genome sequencing	9
exo probe	9
reverse primer	9
bad laci	9
etiological agent	9
inoculated control	9
chinese cakovs	9
multiplex pcr	9
companion animals	9
membrane protein	9
difficile isolates	9
orally inoculated	9
standard deviation	9
assay using	9
analysis based	9
two different	9
vaccination status	9
medicinal plants	9
bcov infection	9
south dakota	9
many different	9
treatment effect	9
detecting pedv	9
feed ingredients	9
fold serial	9
tissue culture	9
weaning mortality	9
january th	9
genbank accession	9
multiple pathogens	9
exact test	8
old female	8
bone marrow	8
several studies	8
cpv dna	8
added value	8
disease severity	8
inoculated intraperitoneally	8
control mice	8
epithelial lesions	8
high degree	8
clinical examination	8
phenotypic characterization	8
resistant lines	8
nsp elisa	8
sense rna	8
based surveillance	8
measured using	8
gross lesions	8
microbiota transplantation	8
clinical serum	8
performed according	8
clinically affected	8
aa substitutions	8
significant increase	8
virus replication	8
pedv attenuated	8
step rt	8
cell death	8
potential risk	8
infectious bovine	8
active ingredient	8
visitors per	8
manuscript preparation	8
coinfected pigs	8
within one	8
high genetic	8
time effect	8
neonatal calves	8
production systems	8
pedv rt	8
ab test	8
two months	8
microbiota community	8
diarrhea panel	8
positive pcr	8
weaned pigs	8
reading frame	8
triandra extract	8
data generated	8
biosecurity measures	8
also found	8
first case	8
using one	8
animal shelter	8
fetal bovine	8
strains obtained	8
inhibited prrsv	8
raw data	8
extraction kit	8
pedv wild	8
species present	8
magnesium acetate	8
dengue virus	8
obtained using	8
regression analysis	8
ibrv dna	8
early stage	8
significant economic	8
morbidity ratio	8
infected control	8
ingenuity pathway	8
results indicate	8
outer membrane	8
testing positive	8
classification system	8
porcine parvovirus	8
laboratory analysis	8
pcv induced	8
lymphoid tissue	8
expression plasmid	8
dna copy	8
gene sequence	8
body weight	8
natural suckling	8
larger whole	8
community structure	8
situ hybridization	8
protein expression	8
phosphate buffered	8
emerging infectious	8
disease resistance	8
gnotobiotic pigs	8
fecal iga	8
significant treatment	8
pcr using	8
noncompliant cases	8
data obtained	8
expression levels	8
piro score	8
mycoplasma bovis	8
quantified using	8
culture supernatant	8
difficile antigen	8
mg kg	8
phase proteins	8
detection limits	8
professional visits	8
antibody levels	8
ibv ab	8
care professionals	8
field samples	8
first report	8
calf diarrhea	8
dependent manner	8
social dilemma	8
molecular analysis	8
significant association	8
female balb	8
spiral reaction	8
small ruminants	8
reference sequences	8
wide range	8
dublin aroa	8
respiratory diseases	8
days old	8
ha gene	8
syndrome coronavirus	8
universal real	8
cdna synthesis	8
swine bioassay	8
bovine rhinotracheitis	8
way anova	8
medium containing	8
large dairy	8
social network	8
franklin lakes	8
ad libitum	8
family coronaviridae	7
chlamydia suis	7
serum albumin	7
brsv infection	7
pedv antibodies	7
coronaviridae family	7
virus isolates	7
using rt	7
mtt assay	7
strain vr	7
showed clinical	7
management practices	7
animal diseases	7
chlamydial infection	7
pseudorabies virus	7
induced mitochondrial	7
canine enteric	7
tool helps	7
false negative	7
infection patterns	7
infectious virus	7
fold higher	7
newly developed	7
serological tests	7
quarantine measures	7
journal submit	7
serum antibodies	7
study provides	7
severe diarrhea	7
stock pedv	7
czech republic	7
per sample	7
pbs containing	7
bacterial infections	7
new zealand	7
using graphpad	7
roc analysis	7
animals included	7
using serum	7
systemic inflammatory	7
time reverse	7
nucleotide identity	7
inflammatory bowel	7
relevant journal	7
polymerase spiral	7
microplate reader	7
dog owners	7
accept pre	7
study revealed	7
dairy herd	7
antibiotic therapy	7
expression differences	7
challenged orally	7
different purposes	7
three dogs	7
two lines	7
vero cells	7
isolation kit	7
viral enteritis	7
high fever	7
infection may	7
clinical symptoms	7
agar plates	7
pathogenic avian	7
research institute	7
reactive protein	7
pcr diarrhea	7
major cause	7
samples taken	7
reported previously	7
coronavirus spike	7
strain isolated	7
gastrointestinal tract	7
strain df	7
pathway analysis	7
muench method	7
host cell	7
phenolic content	7
swine population	7
gastrointestinal disease	7
trade network	7
cdv strains	7
first week	7
active ingredients	7
serum sample	7
secondary antibodies	7
sandwich elisa	7
dna polymerase	7
dgge analysis	7
intestinal contents	7
bovine coronaviruses	7
fiv infections	7
viral loads	7
optical density	7
exotic animal	7
sappan extracts	7
porcine deltacoronavirus	7
datasets used	7
interferon lambda	7
large intestine	7
hemorrhagic fever	7
considered significant	7
high levels	7
stabilized rice	7
vaccine candidates	7
higher level	7
study showed	7
animal trade	7
emblica extract	7
apparently new	7
high number	7
health surveillance	7
noncompliance rate	7
two separate	7
wild type	7
different strains	7
submission inquiries	7
human health	7
bowel disease	7
bcov positive	7
us porcine	7
dna per	7
post infection	7
microscopic lesions	7
disease event	7
shelter rabbits	7
bordetella bronchiseptica	7
species detected	7
positive reactions	7
inhibit prrsv	7
increased significantly	7
showed high	7
horseradish peroxidase	7
selector tool	7
plasmid ps	7
antiviral effects	7
graphpad software	7
two strains	7
japanese pedv	7
immune organs	7
showed positive	7
enterica serotype	7
clinical diagnosis	7
lymphoid tissues	7
bovine torovirus	7
epidemiologic investigation	7
high level	7
following consumption	7
two cats	7
highly sensitive	7
serotype choleraesuis	7
analysis version	7
amplification assays	7
among swine	7
gastrointestinal microbiota	7
culture plates	7
lf probe	7
spray volume	7
wean units	7
foetus infection	7
choleraesuis vector	7
negative sample	7
oral route	7
genus alphacoronavirus	7
genome sequences	7
ibrv rpa	7
either pya	7
scoring system	7
inoculated mice	7
antibody detection	7
sequence identity	7
small number	7
differ significantly	7
agricultural sciences	7
genetic analysis	7
viral evolution	7
pedv universal	7
dublin expressing	7
elisa kit	7
unclear whether	7
may contribute	7
compound gsno	7
new syndrome	7
reston ebolavirus	7
buffy coat	7
expression vector	7
significant hits	7
trade networks	7
enteric coronavirus	6
adapted isolates	6
flow cytometer	6
cats sampled	6
neonatal pigs	6
also performed	6
kg day	6
intestinal epithelial	6
owned dogs	6
three different	6
minimum value	6
vaccine vector	6
additional studies	6
randomly assigned	6
pig iga	6
dairy herds	6
transcription polymerase	6
oxygen species	6
reactive oxygen	6
dgge positive	6
noncompliance rates	6
detect antibodies	6
american association	6
four herds	6
premix ex	6
figures refer	6
bursal disease	6
teaching hospital	6
specific igg	6
three days	6
recurrent cdi	6
vaccine development	6
positive correlation	6
viral genomic	6
differential diagnosis	6
also present	6
intestinal parasites	6
sequence type	6
pet food	6
case presentation	6
younger dogs	6
designed based	6
samples tested	6
srb group	6
felv infection	6
partial least	6
gsno treatment	6
nsp protein	6
pedv infected	6
oxidative stress	6
fold dilutions	6
infective dose	6
corresponding countries	6
low mortality	6
membrane colour	6
modified eagle	6
diarrheic samples	6
pedv exposure	6
clinical examinations	6
infectious peritonitis	6
infected piglets	6
high seroprevalence	6
winter dysentery	6
poultry industry	6
highly connected	6
test results	6
tested pedv	6
serum dilution	6
japanese pedvs	6
left column	6
critically revised	6
also evaluated	6
molecular characterisation	6
organic certified	6
generating compound	6
viral nucleic	6
one week	6
iga responses	6
enteric viruses	6
biosecurity routines	6
unique amino	6
european union	6
washed three	6
tissue specimens	6
two aa	6
cell line	6
double antibody	6
two major	6
final extension	6
outer tube	6
dairy farms	6
pcr negative	6
reproductive failure	6
assays used	6
feline infectious	6
western blotting	6
pathogenic potential	6
supporting information	6
also detected	6
cdv shedding	6
cell control	6
recent outbreaks	6
years old	6
hoof temperatures	6
sterile saline	6
times higher	6
detection range	6
surveillance systems	6
clinical cases	6
different clinical	6
enteric infection	6
natural infection	6
bcov strains	6
swine farms	6
tree based	6
identified using	6
enteric infections	6
pedv antigen	6
allantoic fluid	6
dna amplification	6
assay variability	6
control strategies	6
takara co	6
seven thai	6
data supporting	6
variant porcine	6
dgge negative	6
cats testing	6
neonatal porcine	6
samples submitted	6
also identified	6
dairy cows	6
northwestern germany	6
data set	6
mixed infections	6
simple method	6
stranded dna	6
industry worldwide	6
phase protein	6
infectious bursal	6
brsv prevalence	6
snap test	6
tract infection	6
lung scores	6
movement across	6
environmental conditions	6
diagnostic tests	6
highly effective	6
right column	6
paulo state	6
swedish livestock	6
animal shelters	6
one month	6
following formula	6
virus type	6
cytopathic effect	6
crp mutation	6
animal models	6
different genotypes	6
strains detected	6
nucleocapsid gene	6
pedigree status	6
antiviral effect	6
infected tbln	6
enteropathogenic bacteria	6
immune cells	6
fatty acid	6
time interaction	6
considered positive	6
resistant birds	6
group received	6
incomplete vaccination	6
diarrhoea syndrome	6
breeding herds	6
sensitivity analysis	6
mouse model	6
naked eye	6
visual detection	6
constructed using	6
taqman real	6
positive rates	6
among cats	6
laying hens	6
asian non	6
animal practice	6
trade patterns	6
pams treated	6
pedv infections	6
lavage fluid	6
relationships among	6
subclinical infection	6
community membership	6
diarrhoeic samples	6
commercial vaccine	6
positive cases	6
study will	6
antibody titer	6
mucous membrane	6
changes observed	6
cycle threshold	6
south america	6
stimulated genes	6
two weeks	6
hybridoma cells	6
typhimurium isolates	6
forming units	6
neutralization assay	6
next generation	6
first detection	6
live animal	6
maximum concentration	6
porcine diarrhoea	6
amplified products	6
also demonstrated	6
health approach	6
maximum value	6
tested cdv	6
construct reliability	6
ex taq	6
vaccination history	6
pcv effect	6
based diets	6
least squares	6
resistant strains	6
virulence genes	6
thermo scientific	6
animal use	6
diagnostic methods	6
assessed using	6
previous work	6
exotic animals	6
sequence alignment	6
antibodies using	6
supplementary information	6
milk intake	6
commonly used	6
expressed genes	6
canine parvoviruses	6
toxin gene	6
time quantitative	6
molecular weight	6
greater collaboration	6
contaminated transport	6
close relationship	6
live vaccine	6
diagnostic testing	6
npms assessment	6
perfringens type	6
indicate trade	6
zip nucleic	6
key role	6
ctr calves	6
cellular immune	6
animal movements	6
disease transmission	6
i real	6
challenge material	6
eagle medium	6
elisa test	6
mm nacl	6
amplification products	6
phage types	6
index cases	6
geographical movement	6
viruses present	6
reading frames	6
factors associated	6
detect pedv	6
study highlights	6
strains collected	6
serum neutralization	6
clinical sample	6
ambient temperatures	6
positive hybridoma	6
genes involved	6
conventional rt	6
newborn calves	6
one large	6
serovar choleraesuis	6
antibody sandwich	6
reference interval	6
viral protein	6
protein expressed	6
significant time	6
intestinal tissues	6
highly specific	6
elisa method	6
new coronavirus	6
endoribonuclease activity	6
primer pair	6
intestinal specimens	6
countries indicate	6
highly expressed	6
like particle	6
weight loss	6
serum igg	6
veterinary teaching	6
ethics committee	6
type infection	6
clinical score	6
iii interferons	6
cells transfected	6
immunosorbent assays	6
fluorescent microsphere	6
nt sequences	6
spiked samples	6
vaccine cv	6
positive controls	6
risk cluster	5
daily health	5
parainfluenza virus	5
recombinant plasmid	5
inoculum buffer	5
florida animal	5
extracted rna	5
quality reads	5
time point	5
following infection	5
cuffdiff output	5
dublin vaccine	5
receiver operating	5
early stages	5
tract samples	5
culture infective	5
sequencing data	5
comparative analysis	5
sterile water	5
medical history	5
post inoculation	5
common goals	5
poultry markets	5
bionote test	5
blood cells	5
virus titre	5
serum biochemical	5
detected pedv	5
diagnostic specificity	5
oral immunization	5
diarrhoeic dogs	5
whitney test	5
prevalence rates	5
developing countries	5
final concentration	5
rna extracted	5
plant extract	5
negative swine	5
random effect	5
foster city	5
zoonotic diseases	5
normal spatial	5
positive sample	5
tract infections	5
amplification product	5
significantly decreased	5
organ failure	5
organ systems	5
frequently detected	5
pcv antigen	5
laboratory animal	5
acid amplification	5
weight gain	5
dairy farm	5
conserved among	5
humoral immune	5
antibody response	5
different phage	5
tiliacora triandra	5
antimicrobial agents	5
adipose tissue	5
positive animals	5
us strains	5
candidate genes	5
values ranging	5
diagnostic imaging	5
whole blood	5
asymptomatic prrsv	5
poultry traded	5
significant positive	5
rpa reaction	5
critical importance	5
like lineage	5
red signal	5
breeding stock	5
viruses isolated	5
spike glycoprotein	5
control sirna	5
paired fecal	5
target sequences	5
may result	5
subsequent experiments	5
red line	5
data revealed	5
lymphocyte proliferation	5
protein chip	5
test line	5
dna template	5
false positive	5
protein concentration	5
significantly increased	5
within days	5
us compared	5
control study	5
control birds	5
scan statistic	5
piro scoring	5
app response	5
study demonstrated	5
domestic pigs	5
dakota state	5
blood biochemistry	5
coronavirus associated	5
different concentrations	5
lysis buffer	5
tested negative	5
colour alteration	5
enteric microbiota	5
positive rate	5
false discovery	5
cdv strain	5
low prevalence	5
average number	5
organic dairy	5
retrospective study	5
brings us	5
breed dogs	5
health initiative	5
inflammatory cytokines	5
laboratory animals	5
free feed	5
clinical manifestations	5
co atmosphere	5
dogs showed	5
new insights	5
sodium pentobarbital	5
salmonella serovars	5
factors influencing	5
triplex realtime	5
national center	5
well cell	5
toxigenic strains	5
created using	5
biotechnology information	5
prrsv genome	5
multiple infections	5
collected daily	5
early infection	5
rest period	5
pedv variants	5
significantly reduced	5
antibody titres	5
virus evaluation	5
sdsu adrdl	5
faecal solution	5
brsv seroprevalence	5
commercial swine	5
nasal samples	5
fever clinical	5
gastrointestinal signs	5
response rate	5
information accompanies	5
ort infection	5
neonatal mortality	5
igg titer	5
detection rate	5
viral diseases	5
one step	5
four major	5
prepared using	5
mean ct	5
inhibit pcv	5
farrowing unit	5
outbreak among	5
live mixed	5
normal feces	5
may provide	5
pcr reactions	5
dsh cats	5
vetsuisse faculty	5
viral proteins	5
year period	5
rna negative	5
detection results	5
using primers	5
human healthcare	5
mouse igg	5
also included	5
culture medium	5
biotech co	5
band neutrophils	5
taqman probe	5
geographical location	5
prrsv activity	5
routine use	5
specificity analysis	5
unvaccinated dogs	5
four months	5
fecal specimens	5
potential enteropathogens	5
high throughput	5
data recording	5
three nasal	5
low specificity	5
examined using	5
genetic complexity	5
may indicate	5
respiratory infections	5
coronary band	5
diagnostic techniques	5
parasitological tests	5
interferon alpha	5
vaccine groups	5
adapted isolate	5
index piglet	5
uk cats	5
rapid method	5
nucleotide identities	5
viral isolation	5
positive dgge	5
hev antigen	5
antibiotic treatment	5
another study	5
acid tolerance	5
mean maximum	5
dendritic cells	5
see text	5
cleaved caspase	5
mini kit	5
chinese strains	5
noise ratio	5
mediating effect	5
treated group	5
roche diagnostics	5
detection methods	5
apparently healthy	5
detection using	5
septic shock	5
acute diarrhea	5
mice infected	5
transcription recombinase	5
african swine	5
information regarding	5
colostrum samples	5
see table	5
also used	5
indel type	5
first immunization	5
positive signals	5
starter feed	5
analysed using	5
large variation	5
cecal microbiota	5
infrared thermography	5
one virus	5
multilocus sequence	5
analysis indicated	5
aa differences	5
antimicrobial product	5
ellagic acid	5
poultry flocks	5
virus date	5
samples originated	5
sequence typing	5
acid sequences	5
bacterial pathogens	5
result showed	5
communes included	5
animal origin	5
sample solution	5
samples analysed	5
square test	5
delayed attenuation	5
heart rate	5
diarrheic episode	5
via natural	5
winter months	5
pcr tests	5
study area	5
strong evidence	5
faecal examination	5
mm tris	5
reduce viral	5
primer pairs	5
dispar pcr	5
infection rate	5
milk refusal	5
od value	5
routine diagnostic	5
developed rt	5
viruses associated	5
primer design	5
food chain	5
ibrv lfd	5
pcr amplification	5
sheep blood	5
spread among	5
cakov canine	5
antibody test	5
prdc pigs	5
third week	5
intestinal samples	5
experimentally inoculated	5
rest breaks	5
vaccinated calves	5
genomic sequences	5
ha vi	5
piglet rearing	5
commune network	5
gene amplification	5
finisher pigs	5
nsp gene	5
recombinant attenuated	5
attenuated live	5
negative results	5
maio island	5
summer months	5
typhimurium strain	5
national animal	5
fisher scientific	5
day study	5
ifa specific	5
significantly inhibit	5
purdue strains	5
flow cytometry	5
dilution buffer	5
live salmonella	5
professionals will	5
since pedv	5
expression profiles	5
bronchoalveolar lavage	5
mononuclear cells	5
useful tool	5
dogs aged	5
major acute	5
magna pure	5
allowed us	5
diagnostic tool	5
antiviral response	5
rna polymerase	5
diversity among	5
detection limit	5
negative pcr	5
cells infected	5
animal model	5
vaccine candidate	5
excel spreadsheet	5
sodium dodecyl	5
infected swine	5
porcine plasma	5
one unclassified	5
gallic acid	5
akkermansia muciniphila	5
mean pmr	5
donor gsno	5
disease caused	5
phosphorylation sites	5
capture antibody	5
showed similar	5
biological pathways	5
toxoplasma gondii	5
initial examination	5
disease research	5
old dogs	5
also positive	5
mean concentration	5
isolate js	5
factors may	5
dried porcine	5
rf ph	5
veterinary institute	5
vitro anti	5
coronavirus infections	5
samples obtained	5
capillary refill	5
analyses showed	5
trc promoter	5
genetic drift	5
influenza viruses	5
tracheobronchial lymph	5
communes linked	5
wash buffer	5
susceptibility testing	5
low sensitivity	5
professional associations	5
animals sampled	5
breaths min	5
phase response	5
enteropathogenic escherichia	5
economic loss	5
four samples	5
amplification efficiency	5
clinical laboratory	5
milk replacers	5
snr value	5
seven days	5
np specific	5
eastern china	5
affinity chromatography	5
classical strains	5
calves presented	5
possible source	5
results revealed	5
fusion proteins	5
viral enteric	5
higher sensitivity	5
sirnas targeting	5
frugal tree	5
first study	5
control vector	5
positive sera	5
year old	5
coating antigen	5
plasmid pcdna	5
oral administration	5
detection antibody	5
cap protein	5
passive transfer	5
line i	5
using molecular	5
predicted amino	5
veterinary practitioners	5
cdv infection	5
environmental contamination	5
iif values	5
refill time	5
present work	5
metronidazole resistance	5
ml ifn	5
pedv transmission	5
written informed	5
significantly associated	5
clinical course	5
positive cells	5
primary outbreak	5
least two	5
complete genomes	5
rabbit health	5
strains containing	5
upper east	5
provide proof	5
life cycle	5
enteric virus	5
human patients	5
clayton tree	5
distemper outbreak	5
wt vol	5
caesalpinia sappan	5
also observed	5
diagnostic laboratories	5
distilled water	5
veterinary medical	4
salmonella sp	4
nc mice	4
cross reactivity	4
diagnostic applications	4
corona virus	4
viral antigen	4
within flow	4
trade pattern	4
first reported	4
haemagglutination inhibition	4
swabs collected	4
known enteric	4
intensive systems	4
strand cdna	4
different pedv	4
mean number	4
positive thoughts	4
virus seroprevalence	4
gene sequencing	4
affected herds	4
large bowel	4
infectious agent	4
using anti	4
field study	4
three hundred	4
field conditions	4
results presented	4
within europe	4
diseases caused	4
using recombinase	4
rehydration buffer	4
reaction volume	4
diluted serum	4
integrated poly	4
site detection	4
low concentration	4
treated groups	4
feed bins	4
type cdv	4
union council	4
dodecyl sulfate	4
family farms	4
cells cultured	4
day period	4
induced apoptosis	4
national institutes	4
production system	4
cell apoptosis	4
investigated outbreaks	4
disease center	4
professional groups	4
secondary infection	4
dogs fed	4
low levels	4
virus spread	4
two methods	4
developed countries	4
prrs virus	4
centrifuge tube	4
arteritis virus	4
lung score	4
also cause	4
severe disease	4
rumen fermentation	4
prevalent pathogen	4
using cell	4
different countries	4
using protocols	4
parvoviral enteritis	4
resistant line	4
toxigenic pm	4
copies reaction	4
national veterinary	4
two samples	4
month follow	4
virus among	4
study shows	4
acid changes	4
enterohemorrhagic escherichia	4
avian viruses	4
molecular diversity	4
microscopic evaluation	4
mortality associated	4
assay outcomes	4
biochemistry results	4
rapidly across	4
eight samples	4
glycosylation site	4
acid sequence	4
factors considered	4
good outcome	4
strain dx	4
fecal flotation	4
using primer	4
np protein	4
difficile toxin	4
equal volume	4
host responses	4
bowel diarrhoea	4
host innate	4
beef cattle	4
one case	4
two vaccine	4
dna sequencing	4
lda scores	4
virus may	4
sars coronavirus	4
cystoisospora suis	4
study describes	4
university animal	4
surface protein	4
pig industry	4
southern brazil	4
pure lc	4
phylum level	4
mg dl	4
cytokine production	4
griess reaction	4
subsequent outbreaks	4
stable maintenance	4
contact structure	4
chemical structure	4
analysis tool	4
small family	4
viral particles	4
low cost	4
single infections	4
china agricultural	4
may occur	4
epitope region	4
poor body	4
fimbrial genes	4
red color	4
false positives	4
higher mortality	4
published article	4
coronavirus live	4
average noncompliance	4
environmental factors	4
high brsv	4
pathogenic porcine	4
dna virus	4
washing steps	4
ffn assay	4
clavulanic acid	4
reaction assay	4
average temperature	4
neonatal dairy	4
rearing facility	4
antioxidant activity	4
survival rate	4
probebased real	4
sheep nasal	4
biosecurity procedures	4
pathogens involved	4
optimal dilution	4
southern italy	4
coagulation dysfunction	4
atrophic rhinitis	4
calf health	4
synthesis kit	4
social categorization	4
highly significant	4
phase high	4
human isolates	4
generated using	4
gut microbiome	4
salmonella vaccine	4
distance among	4
taken place	4
threshold temperature	4
specific antibody	4
lung samples	4
minimum essential	4
ethical approval	4
gnotobiotic piglets	4
food inspection	4
strains used	4
operating characteristic	4
cakov positivity	4
rice protein	4
nasal discharge	4
developed based	4
hela cells	4
livestock trade	4
plating serial	4
samples containing	4
asian countries	4
enteric diseases	4
animal resource	4
taxonomic classification	4
predisposing factors	4
vitro antiviral	4
veterinary medicinal	4
vp genotypes	4
porcine viruses	4
cytokine genes	4
week periods	4
study confirmed	4
previous page	4
moderate disease	4
parsimony test	4
biological samples	4
perfringens toxin	4
standard deviations	4
rpa primer	4
lethal outcome	4
calculated based	4
circovirus associated	4
associated lymphoid	4
per well	4
high susceptibility	4
specific pathogen	4
ovipneumoniae dna	4
rural affairs	4
dgge method	4
commonly reported	4
condensed tannins	4
proline rich	4
dnt ab	4
indirect enzyme	4
nucleotide deletion	4
discovery rate	4
antibody negative	4
partial effect	4
daily basis	4
causes significant	4
synonymous mutations	4
test based	4
dog population	4
canine patients	4
pregnant sows	4
beta receptor	4
neutralising antibodies	4
host range	4
classical japanese	4
recurrent outbreaks	4
risk clusters	4
three control	4
biochemical analysis	4
structural protein	4
indel variants	4
pedv immunized	4
provides evidence	4
protein intimin	4
feedlot cattle	4
two novel	4
may therefore	4
agent responsible	4
viral entry	4
genes encoding	4
hind iii	4
critical role	4
affected animals	4
standard pedv	4
recorded throughout	4
least times	4
recent study	4
goat sequences	4
eu countries	4
study also	4
immunized groups	4
transcriptase polymerase	4
serodiagnosis systems	4
virus using	4
geographical areas	4
observed among	4
body condition	4
market veterinary	4
isospora spp	4
mice treated	4
unweighted unifrac	4
protective efficacy	4
hospital mortality	4
recombinant proteins	4
likely source	4
market surveillance	4
without clinical	4
phosphate buffer	4
cattle farms	4
commercial test	4
diluted ibrv	4
particle associated	4
synergistic effects	4
received pigs	4
northern california	4
rabbits kept	4
analysis revealed	4
iga levels	4
dna using	4
salmonella strains	4
better understand	4
day old	4
animal veterinary	4
transport vehicles	4
available data	4
virus particles	4
focus neutralization	4
kansas state	4
datasets generated	4
potential candidate	4
eaea gene	4
three mycoplasma	4
based pcr	4
included within	4
ort isolates	4
stool consistency	4
infections relative	4
total nucleic	4
intestinal flaccidity	4
tested compounds	4
pcr testing	4
cell epitopes	4
receptor binding	4
fluorescent signals	4
igg antibodies	4
mixed infection	4
livestock movements	4
respiratory bovine	4
five piglets	4
showed signs	4
manuscript revision	4
one another	4
transcription loop	4
analysis using	4
similar pathological	4
monophasic typhimurium	4
resource wing	4
livestock farms	4
respiratory complex	4
canine parvo	4
partial vp	4
pig farmers	4
commercial inactivated	4
data provide	4
contagious diseases	4
affected breeding	4
data acquisition	4
investigate whether	4
prism software	4
high frequency	4
recent studies	4
four small	4
using monoclonal	4
new variants	4
comprehensive review	4
private veterinary	4
secondary outbreaks	4
like particles	4
tolerance response	4
enteric zoonotic	4
innate immunity	4
pcv co	4
healthy control	4
perfringens enterotoxin	4
strongyloides ransomi	4
disease outbreaks	4
three monophasic	4
fifteen minutes	4
cynomolgus macaques	4
new variant	4
vaccination rate	4
birds infected	4
two viruses	4
hong kong	4
outbreak risk	4
vector pet	4
geographic location	4
chlamydophila psittaci	4
aa level	4
commercial poultry	4
two new	4
containing fetal	4
experimental challenge	4
giant weak	4
attending veterinarian	4
knowledge base	4
metagenomic analyses	4
drug treatment	4
maximum likelihood	4
sodium bicarbonate	4
diagnostic approach	4
bovirhinis respectively	4
kept still	4
specific information	4
control sera	4
central european	4
pathogen detection	4
fetal calf	4
antibiotic resistance	4
equine arteritis	4
susceptible animals	4
may explain	4
different time	4
positive relation	4
health assessment	4
several factors	4
bacterial strains	4
amoxicillin clavulanic	4
reaction time	4
extended period	4
epidemic waves	4
different animals	4
enveloped single	4
study conception	4
blot using	4
ecor i	4
countries including	4
finishing pigs	4
animals infected	4
sows showed	4
different regions	4
animal experiment	4
feline salmonellosis	4
purified rf	4
amplification recombinase	4
commune infection	4
observed within	4
determine whether	4
skimmed milk	4
one infection	4
microsphere immunoassay	4
peroxidase conjugate	4
multiplex real	4
virus identification	4
still unclear	4
alpha beta	4
poultry rmbd	4
poultry production	4
among pedv	4
kindly supplied	4
like disease	4
family arteriviridae	4
antimicrobial drugs	4
new antigenic	4
pcr test	4
prediction accuracy	4
antigen synthesis	4
oral fluid	4
weak component	4
diarrhoeic animals	4
strains showed	4
statistical computing	4
low risk	4
virus isolated	4
diarrheic group	4
hour rest	4
clinical data	4
supplementary file	4
haemorrhagic diarrhoea	4
etiological diagnosis	4
rna genome	4
prototype strain	4
strains available	4
experimental conditions	4
different geographical	4
isothermal dna	4
joining phylogenetic	4
fingerprint analysis	4
animal husbandry	4
amplification methods	4
lb broth	4
infection rates	4
immunochromatographic assay	4
live birds	4
sterile phosphate	4
explanatory variables	4
results suggested	4
animal hospitals	4
valuable tool	4
infected cattle	4
essential medium	4
calf serum	4
assay targeting	4
shiga toxin	4
flaccid intestines	4
also confirmed	4
pathogen introduction	4
four days	4
several dogs	4
clinical practice	4
saoa protein	4
distribution among	4
competing interest	4
immunochromatographic strip	4
one herd	4
animal ethics	4
seven dead	4
membrane fusion	4
infected mice	4
ampure xp	4
may influence	4
prrsv isolate	4
pcr kit	4
rna isolation	4
food animal	4
expressing intimin	4
large scale	4
early detection	4
log prrsv	4
livestock units	4
suspected cases	4
different animal	4
cycling parameters	4
filled stomachs	4
santa clara	4
human salmonellosis	4
disease associated	4
recombinant expression	4
gsno plus	4
ngs approach	4
reference strain	4
purification kit	4
nervous system	4
secondary bacterial	4
reference viruses	4
cellular processes	4
first days	4
experimental design	4
test strips	4
sample collected	4
similar clinical	4
values equal	4
enteric pathogen	4
distinguish pedv	4
every day	4
heat stabilized	4
affected pigs	4
surveillance programs	4
shelter rabbit	4
pig production	4
pcr method	4
bearing rebov	4
pathogens may	4
polyacrylamide gel	4
dogs may	4
critical review	4
thank prof	4
lung injury	4
mean age	4
transcription factor	4
four different	4
plated onto	4
uninfected animals	4
mediation effects	4
recurrent clostridium	4
insect cells	4
diarrheal disease	4
grand island	4
biological networks	4
ai outbreaks	4
several european	4
amplification method	4
using two	4
boehringer ingelheim	4
ultrapure water	4
nt antibody	4
causative agents	4
envelope protein	4
three weeks	4
species infection	4
meat rabbit	4
experimental studies	4
milk production	4
coinfected piglets	4
score means	4
thawing three	4
control interventions	4
molecular mechanisms	4
control standards	4
livestock industry	4
primers probe	4
parvoviral dna	4
three assays	4
zoonotic transmission	4
government testing	4
work together	4
serological diagnosis	4
still present	4
potentially pathogenic	4
heterologous antigen	4
designed using	4
biosafety level	4
blood mononuclear	4
already reported	4
cdv vaccination	4
bp fragment	4
conflicting results	4
one day	4
stranded positive	4
slightly higher	4
blue circles	4
intimin might	4
independent experiments	4
sequences per	4
hi titre	4
factors involved	4
taking place	4
attenuated cv	4
food safety	4
domestic dog	4
also considered	4
pathogen free	4
stool conditions	4
relative excess	4
checkerboard titration	4
inactivated vaccine	4
values based	4
published data	4
control samples	4
first line	4
severe dehydration	4
decreased significantly	4
still unknown	4
first described	4
coli isolates	4
eye temperature	4
nanjing agricultural	4
one sample	4
study population	4
mycoplasma agalactiae	4
like strains	4
sequence identities	4
finish pigs	4
overarching identity	4
well known	4