trigram

This is a table of type trigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

trigram	frequency
infectious bronchitis virus	2945
of infectious bronchitis	1073
avian infectious bronchitis	1069
of avian infectious	538
of the s	484
in this study	465
the s gene	463
the presence of	366
coronavirus infectious bronchitis	342
the s protein	324
in the s	306
based on the	301
analysis of the	293
as well as	293
bronchitis virus in	287
according to the	285
of the ibv	278
of the virus	277
mouse hepatitis virus	236
ck ch ldl	234
s gene of	198
infectious bronchitis viruses	195
of the coronavirus	188
infectious bronchitis coronavirus	184
of the genome	180
the spike protein	178
polymerase chain reaction	173
end of the	173
the s subunit	172
severe acute respiratory	171
acute respiratory syndrome	170
region of the	168
was used to	158
the n protein	155
avian coronavirus infectious	154
of the spike	149
the detection of	147
ch ldl i	146
showed that the	146
sequence of the	146
in order to	144
sequences of the	143
liu et al	143
the present study	139
all of the	137
in the trachea	137
a and b	135
the use of	133
of the viral	130
has been shown	129
detected in the	127
strains of ibv	126
it has been	125
were infected with	124
data not shown	124
of ibv in	123
of the n	123
one of the	123
were used to	123
the s glycoprotein	122
been shown to	122
the emergence of	121
of avian coronavirus	121
the poultry industry	120
respiratory syndrome coronavirus	119
s and s	119
ibv n protein	118
of a novel	116
amino acid sequences	115
the respiratory tract	115
compared to the	114
the coronavirus infectious	114
infected with ibv	112
due to the	112
observed in the	111
molecular characterization of	111
cells infected with	110
closely related to	110
embryonated chicken eggs	110
was carried out	110
to determine the	110
characterization of the	110
the ck ch	108
the development of	108
s protein of	107
most of the	107
the number of	106
newcastle disease virus	106
and n genes	105
shown in fig	105
strain of ibv	105
and ck ch	104
found to be	104
for the detection	103
of mouse hepatitis	103
c for min	103
the n gene	102
the amino acid	101
coronavirus avian infectious	101
gene of the	100
were observed in	100
strain ck ch	99
in the presence	99
a total of	98
as previously described	98
the absence of	98
was detected in	97
the m protein	97
the expression of	96
of ibv and	96
sequence analysis of	95
the results of	95
for h at	94
phylogenetic analysis of	94
in the present	93
in addition to	92
as shown in	92
pcr products were	91
the cells were	91
amino acid sequence	91
shown to be	90
s and n	90
ck ch lhlj	89
was observed in	88
results showed that	88
of ibv strains	87
were used for	87
compared with the	86
changes in the	86
that of the	86
in the same	86
bronchitis virus and	85
in the spike	85
of s gene	85
p and p	85
in the field	84
of a new	84
the genome of	84
were collected from	84
cavanagh et al	84
rna was extracted	84
to that of	83
the role of	83
cells were infected	83
comparison of the	83
the effect of	83
strain of infectious	83
as described previously	82
nephropathogenic infectious bronchitis	82
related to the	81
a role in	81
on the s	81
and characterization of	81
bronchitis virus isolates	81
the s and	81
each of the	80
the formation of	80
was used for	80
with infectious bronchitis	79
chickens infected with	79
and amino acid	79
identification of a	79
were detected in	79
in embryonated eggs	78
responsible for the	78
at days post	77
the middle east	77
involved in the	77
at room temperature	77
the sequence of	77
the end of	76
protein of ibv	76
for min at	76
the spike glycoprotein	76
were obtained from	75
role in the	75
located in the	75
the ibv genome	75
used in this	74
and n proteins	74
found in the	74
in recent years	73
a number of	73
against infectious bronchitis	73
the avian coronavirus	72
detection of infectious	72
in the ibv	71
spike protein of	71
bronchitis virus isolated	71
of the protein	71
bronchitis virus strain	71
s subunit of	71
an important role	71
bronchitis virus from	70
was performed using	70
was used as	70
and identification of	70
transmissible gastroenteritis virus	70
of the m	70
characterization of a	70
the spike gene	69
the infectious bronchitis	69
used for the	69
the viral genome	69
were found to	69
important role in	69
the virus was	68
associated with the	68
those of the	68
part of the	68
the trachea and	68
in the middle	68
has not been	67
vero cells were	67
the control of	67
was extracted from	67
infectious bronchitis in	67
gene of ibv	67
of the two	67
avian influenza virus	65
s gene sequence	65
viral rna was	65
e and m	65
some of the	65
virus in the	65
casais et al	65
sequencing of the	64
was determined by	64
this study was	63
the generation of	63
expression of the	63
in the control	63
in the respiratory	62
used in the	62
nucleotide and amino	62
the nucleocapsid protein	62
of the avian	62
to evaluate the	62
differences in the	62
ibv strains were	62
regions of the	62
coronavirus spike protein	61
c for s	61
were inoculated with	61
in vero cells	61
codon optimized s	61
in the absence	61
to be a	61
we found that	60
the viral rna	60
ibv and ndv	60
the ibv strains	60
open reading frame	60
similar to the	60
isolated in china	60
it is not	60
infected with the	60
the level of	60
nucleotide sequences of	60
chen et al	60
strains of avian	59
in cells infected	59
the control group	59
play a role	59
of the sequence	59
present in the	59
than that of	59
in each group	58
open reading frames	58
of the recombinant	58
the replicase gene	58
of ibv infection	58
are shown in	58
samples were collected	58
chickens immunized with	58
the production of	58
to infectious bronchitis	58
bronchitis virus is	58
the case of	58
were found in	58
ck ch lsc	58
as a result	58
within the s	57
bronchitis virus infection	57
specific pathogen free	57
avian coronavirus ibv	57
in the case	57
used as a	57
s protein is	57
have shown that	57
genetic diversity of	57
consistent with the	57
of ck ch	57
has been reported	57
is responsible for	56
the i isolate	56
the surface of	56
the birds were	56
ch lsc i	56
the beaudette strain	56
in the viral	56
strains of infectious	56
for the presence	56
ibv strains in	56
gene of infectious	55
was isolated from	55
a determinant of	55
of ibv is	55
and m proteins	55
the replication of	55
of viral rna	55
the genbank database	55
have been reported	55
m and n	55
the complete genome	54
hypervariable region of	54
the allantoic fluid	54
protein is a	54
acid sequences of	54
in the n	54
expression levels of	54
indicated that the	54
the lack of	54
proteins of avian	54
of ibv was	54
old spf chickens	54
and stored at	54
the phylogenetic tree	54
emergence of new	53
in southern china	53
of s protein	53
in contrast to	53
trachea and kidney	53
the united states	53
of the disease	53
well as the	53
was found in	53
ck ch ljl	53
cd and cd	52
the current study	52
was added to	52
for detection of	52
emergence of a	52
ck ch lgx	52
to those of	52
of a coronavirus	52
genome of the	51
such as the	51
amino acid substitutions	51
the pathogenesis of	51
belongs to the	51
and molecular characterization	51
g for min	51
cook et al	51
the pathogenicity of	51
between the two	51
corresponding to the	51
birds in the	51
and efficacy of	50
the hypervariable region	50
subunit of the	50
it is possible	50
infectious bursal disease	50
bronchitis virus strains	50
isolated from the	50
has also been	50
by western blot	50
the results showed	50
and evaluation of	50
the murine coronavirus	49
results of the	49
significant at p	49
n protein was	49
ibv isolates were	49
any of the	49
was performed with	49
of infected cells	49
of the birds	49
recombination in the	49
downstream of the	49
allantoic fluid was	49
the synthesis of	49
the negative control	49
were isolated from	49
in china and	49
in vitro and	49
western blot analysis	49
in terms of	49
in the poultry	48
bronchitis viruses isolated	48
of this study	48
s protein was	48
to identify the	48
the pcr products	48
protein of the	48
the sequences of	48
of the b	48
was found to	48
were incubated at	48
a new genotype	48
when compared to	48
influenza a virus	48
amino acid changes	48
is associated with	48
are associated with	48
presence of ibv	48
is the first	48
as a vaccine	48
were used as	48
serotypes of infectious	48
of vaccination against	47
a result of	47
that the s	47
demonstrated that the	47
clinical signs of	47
not essential for	47
and s proteins	47
of the h	47
to be the	47
a variety of	47
assay for the	47
of an infectious	47
bronchitis virus a	47
virus isolated in	47
shown in table	46
of amino acid	46
at the same	46
each group were	46
the ibv strain	46
clinical signs were	46
detection of ibv	46
on the other	46
the s spike	46
the genomic rna	46
cells transfected with	46
the authors declare	46
the effects of	46
inoculated with the	46
chickens in the	45
at h post	45
strains of the	45
bronchitis virus the	45
the ability of	45
have been identified	45
suggesting that the	45
live attenuated vaccines	45
this is the	45
of severe acute	45
deduced amino acid	45
and evolution of	45
of amino acids	45
of the infectious	45
is a determinant	45
a highly contagious	45
rna recombination in	45
of murine coronavirus	45
in the current	45
the nucleotide sequences	45
member of the	44
is not clear	44
the leader rna	44
this work was	44
obtained from the	44
recombinant s protein	44
mutations in the	44
on the surface	44
with those of	44
characterization of infectious	44
with that of	44
chickens inoculated with	44
structure of the	44
spf chicken eggs	44
this study were	44
for min and	44
as described above	44
the recombinant ibvs	44
the amount of	44
other ibv strains	44
of the gene	44
linked immunosorbent assay	43
virus infection in	43
parts of the	43
at the end	43
the ability to	43
is required for	43
is consistent with	43
protein of avian	43
the other hand	43
in cek cells	43
were treated with	43
as compared to	43
in the chicken	43
were positive for	43
in cultured cells	43
the host cell	43
cloned into the	43
tracheal organ cultures	43
included in the	42
were significantly higher	42
against challenge with	42
presence of the	42
of the other	42
structural proteins of	42
proteins of ibv	42
h n and	42
cells were fixed	42
were challenged with	42
and in vivo	42
of the same	42
suggests that the	42
isolation and identification	42
diversity of avian	42
in our study	42
of ibv m	42
virus isolates from	42
considered to be	42
was supported by	41
feline infectious peritonitis	41
the allantoic cavity	41
of the chickens	41
of the respiratory	41
listed in table	41
viral rna synthesis	41
were performed using	41
variation in the	41
cells were incubated	41
have been shown	41
an infectious bronchitis	41
a member of	41
appears to be	41
the induction of	41
to the poultry	41
infection in the	40
because of the	40
the cell surface	40
specificity of the	40
together with the	40
were incubated with	40
s protein gene	40
bronchitis virus infectious	40
resulted in a	40
derived from the	40
in infected cells	40
indicating that the	40
in the kidneys	40
bronchitis virus variants	40
of the e	40
revealed that the	40
pcr was performed	40
ibv infection in	40
the clinical signs	40
identification of the	40
vitro and in	40
chickens vaccinated with	40
from each group	39
the family coronaviridae	39
detection of the	39
are involved in	39
similar to that	39
n proteins of	39
of the most	39
ch lhlj v	39
caused by the	39
against avian infectious	39
clinical signs and	39
to confirm the	39
can be used	39
there is a	39
development of a	39
ibv pseudoknot rna	39
carried out using	39
performed using the	39
of ibv isolates	39
been shown that	39
coronavirus nucleocapsid protein	39
ibv has been	39
pcr assay for	39
the peplomer protein	39
the importance of	39
found that the	39
understanding of the	38
reverse genetics system	38
field isolates of	38
wang et al	38
s glycoprotein of	38
with the same	38
molecular epidemiology of	38
bronchitis virus by	38
were analyzed by	38
the coronavirus avian	38
s genes of	38
lead to the	38
was shown to	38
is a highly	38
replication in the	38
the occurrence of	38
of the full	38
be due to	38
the method of	38
is not essential	38
to detect the	38
the evolution of	38
for the first	38
spike protein is	38
there was no	38
the vaccinia virus	38
valastro et al	38
the percentage of	38
to determine whether	38
resulted in the	38
of the a	38
the majority of	38
around the world	38
broiler flocks in	37
upstream of the	37
n genes of	37
of ibv n	37
protein gene of	37
the avian infectious	37
middle east respiratory	37
of the rna	37
lesions in the	37
virus isolated from	37
of influenza virus	37
amino acid residues	37
virus infectious bronchitis	37
reed and muench	37
of the murine	37
biology of coronaviruses	37
evaluation of the	37
flocks in china	37
been detected in	37
as described by	37
is the most	37
to investigate the	37
viral load in	37
recombinant avian infectious	37
activity of the	37
belonging to the	37
bronchitis virus vaccine	36
the in vitro	36
there were no	36
days of age	36
in specific pathogen	36
in vaccinated chickens	36
xu et al	36
recombinant infectious bronchitis	36
have been described	36
significantly higher than	36
the vaccine strain	36
the golgi complex	36
essential for replication	36
virus from the	36
the fact that	36
east respiratory syndrome	36
day of age	36
strains isolated in	36
a review of	36
protein of infectious	36
to assess the	36
ibv in the	36
none of the	36
of the five	36
shown that the	36
the molecular biology	36
by infectious bronchitis	36
the first time	36
bronchitis virus s	36
ibv isolates in	36
similar to those	36
as a receptor	36
induced by the	35
that the ibv	35
use of the	35
bronchitis viruses in	35
review of the	35
mrna expression levels	35
cells in the	35
the e protein	35
surface of the	35
wit et al	35
poultry industry worldwide	35
yu et al	35
genome sequences of	35
spf chickens were	35
of ibv strain	35
used to detect	35
murine hepatitis virus	35
were carried out	35
higher than those	35
total rna was	35
hepatitis virus strain	35
de wit et	35
of newcastle disease	35
added to the	35
in egg production	35
ck ch lln	35
change in the	35
identification of avian	35
is possible that	35
strains have been	35
molecular biology of	34
of the viruses	34
the m gene	34
used as the	34
suggested that the	34
the ibv n	34
ch lln i	34
ibv strain mass	34
of a recombinant	34
of the amino	34
be involved in	34
of nephropathogenic infectious	34
replication of the	34
with the ibv	34
cek cells were	34
extracted from the	34
the course of	34
activation of the	34
be associated with	34
in response to	34
siddell et al	34
in the first	34
of the trachea	34
were subjected to	34
is one of	33
the possibility that	33
epithelial cells of	33
but not the	33
and infectious bronchitis	33
s gene and	33
viruses isolated in	33
of e l	33
at c for	33
were transfected with	33
l l chickens	33
the entire genome	33
the ibv m	33
authors declare that	33
the h strain	33
in cells expressing	33
analysis of a	33
were divided into	33
l h chickens	33
of the c	33
li et al	33
the infectivity of	33
effect on the	33
wild type and	33
to ibv infection	33
of the virion	33
play an important	33
cellular immune responses	33
circulating in the	33
evaluation of a	33
protein in the	33
the activation of	33
to the ibv	33
terminus of the	32
of n protein	32
ck ch lsd	32
study was to	32
the nucleotide and	32
was confirmed by	32
results suggest that	32
the virus in	32
protection against the	32
jackwood et al	32
to the s	32
gene sequences of	32
led to the	32
fragment length polymorphism	32
vaccinated challenged birds	32
a novel genotype	32
that they have	32
the viral load	32
expression level of	32
t rna polymerase	32
of the infected	32
were analyzed using	32
the phylogenetic analysis	32
virus was detected	32
like infectious bronchitis	32
economic losses to	32
the samples were	32
and use of	32
the infected cells	32
the interaction of	32
dependent rna polymerase	32
the s region	32
in which the	32
optimized s protein	32
ibv strain ck	32
virus circulating in	32
with ibv and	32
strand rna synthesis	32
and control measures	32
vaccine strain h	32
for the development	32
orf a and	32
of a real	32
tissue tropism of	31
days after inoculation	31
a simple method	31
the concentration of	31
i isolate and	31
korean ibv isolates	31
in the lungs	31
different strains of	31
no significant difference	31
targeted rna recombination	31
in the cytoplasm	31
were collected at	31
the viruses were	31
bronchitis virus circulating	31
the ibv s	31
viral genomic rna	31
site of the	31
conflict of interest	31
strain of avian	31
and analysis of	31
respiratory tract of	31
peplomer protein of	31
chicken embryo kidney	31
and analyzed by	31
h and h	31
analysis showed that	31
the analysis of	31
isolates of avian	31
analysis of infectious	31
were purchased from	31
derived from a	31
expressing codon optimized	31
of the negative	31
positive for ibv	31
depending on the	31
the immune response	31
plays an important	31
of the complete	31
and in the	31
of the three	31
completion of the	31
studies have shown	31
demonstrates that the	31
serotype of infectious	31
chain reaction and	31
ibv n gene	31
recombination within the	31
four structural proteins	31
supported by the	31
bronchitis coronavirus in	31
of these viruses	31
that the virus	31
was detected by	30
the addition of	30
declare that they	30
transcription polymerase chain	30
in the kidney	30
to the emergence	30
fusion activity of	30
ibv strains and	30
which is the	30
yount et al	30
cells were treated	30
in the virus	30
strains ck ch	30
determination of the	30
the e l	30
the in ovo	30
were able to	30
of the orf	30
the most common	30
the viral envelope	30
vaccinated and non	30
the identification of	30
the nonpermissive temperature	30
of virulent ibv	30
higher than that	30
type i interferon	30
losses to the	30
sequences in the	30
zhang et al	30
at the nonpermissive	30
the leader sequence	30
cytoplasmic tail of	30
amino acid identity	30
are listed in	30
at days of	30
suggest that the	30
they have no	30
and has been	30
the virus titer	30
cell fusion activity	30
complete genome sequences	30
of recombination in	30
carried out in	30
the same serotype	30
there was a	30
weeks of age	30
in cell culture	30
for clinical signs	30
of the cell	30
domain of the	30
the mechanism of	30
in the genome	30
is involved in	30
virus replication in	30
innate immune response	30
genes of the	30
members of the	30
have been detected	30
rna in the	30
of influenza a	30
the endoplasmic reticulum	30
addition to the	30
of ibv to	29
high degree of	29
increase in the	29
of ibv were	29
n protein of	29
in both the	29
and incubated for	29
in the genbank	29
been reported in	29
protective efficacy of	29
immune responses in	29
a negative control	29
respiratory disease in	29
the epithelial cells	29
followed by cycles	29
the rate of	29
than those of	29
structural and accessory	29
of an avian	29
with wild type	29
in broiler flocks	29
antibodies against ibv	29
pathogenic avian influenza	29
tree was constructed	29
be used to	29
s glycoprotein gene	29
disease in chickens	29
the chickens were	29
would like to	29
the severity of	29
in the united	29
pathogenicity of a	29
n protein is	29
the loss of	29
replication and transcription	29
in accordance with	29
is a major	29
of the nucleolus	29
fetal bovine serum	29
strains in the	29
the involvement of	29
to avian infectious	29
the size of	29
s coding region	29
group iii coronaviruses	29
human coronavirus e	29
in all of	29
was performed in	29
situation and control	28
molecular evolutionary genetics	28
highly contagious disease	28
the same time	28
room temperature for	28
of ibv h	28
bursal disease virus	28
with the recombinant	28
the prevalence of	28
of the i	28
to examine the	28
work was supported	28
determinant of cell	28
of the chicken	28
were selected for	28
of the first	28
submitted to the	28
the orf a	28
morbidity and mortality	28
immune responses to	28
acid sequence of	28
cells were washed	28
amino acid differences	28
genome sequence of	28
cells were transfected	28
spf embryonated chicken	28
the plasma membrane	28
of the nucleocapsid	28
likely to be	28
rndv expressing codon	28
reverse genetic system	28
a receptor for	28
difference in the	28
in this region	28
s gene was	28
has been demonstrated	28
size of the	28
in the host	28
the levels of	28
of the tracheal	28
b and c	28
the h vaccine	28
epidemiology of infectious	28
are known to	28
characteristics of the	28
evolutionary genetics analysis	28
of chickens with	28
that the spike	28
thought to be	28
referred to as	28
was able to	28
of the peplomer	28
different ibv strains	28
been identified in	28
is essential for	28
nucleotide sequence of	28
contribute to the	28
of the four	28
and pathogenicity of	28
of cell tropism	28
half of the	28
the viral replication	28
the specificity of	28
and s subunits	28
structural protein genes	28
current situation and	28
compared with those	28
egg production and	28
the efficiency of	28
s gene sequences	28
of the oviduct	28
the binding of	28
the isolation and	28
cells were cultured	28
virus in chickens	28
of the isolate	28
an increase in	27
coronavirus mouse hepatitis	27
the birds in	27
may be a	27
and days post	27
indicate that the	27
the percentages of	27
be responsible for	27
based on a	27
of these two	27
of the history	27
in chickens in	27
koch et al	27
h vaccine strain	27
on the peplomer	27
nucleocapsid protein of	27
bronchitis virus resulting	27
isolated in the	27
been isolated from	27
identified in the	27
of ibv are	27
a previous study	27
method of estimating	27
isolates from china	27
the basis of	27
blood samples were	27
a vaccine strain	27
cleavage of the	27
result in the	27
chicken flocks in	27
respiratory disease of	27
the function of	27
were used in	27
human immunodeficiency virus	27
bronchitis virus expressing	27
have also been	27
for the isolation	27
was performed by	27
in the tracheal	27
by cycles of	27
virus and the	27
simple method of	27
the first report	27
old spf chicken	27
old spf embryonated	27
the reaction was	27
gene of avian	27
has been identified	27
in china in	27
bronchitis virus using	27
and orf b	27
of estimating fifty	27
of the structural	27
was isolated in	27
after ibv infection	27
the cytoplasmic tail	27
terminal region of	27
serum samples were	27
analysis was performed	27
proteins of the	27
in vaccinated and	27
the origin of	27
have not been	26
approved by the	26
n gene and	26
reverse transcription polymerase	26
respiratory syndrome vaccine	26
experiences of vaccination	26
were added to	26
studies on the	26
gross lesions were	26
of the ck	26
novel variant of	26
our results showed	26
ldl i p	26
infection in chickens	26
to the golgi	26
embryonated spf chicken	26
were submitted to	26
indicates that the	26
the structure of	26
cells of the	26
poultry and other	26
infectious bronchitis coronaviruses	26
been associated with	26
challenged with ibv	26
infected cek cells	26
economic losses in	26
syndrome vaccine development	26
were identified in	26
at and dpi	26
virus expressing a	26
each time point	26
cells were then	26
complete genome sequence	26
the kidneys of	26
protection against challenge	26
the nucleotide sequence	26
in poultry and	26
to the same	26
resulting from recombination	26
been reported that	26
recombinant vaccinia virus	26
n gene of	26
belonged to the	26
fragment of the	26
development and evaluation	26
spike protein gene	26
three times with	26
avian coronavirus spike	26
were included in	26
with each other	26
in vitro transcription	26
the titer of	26
ibv genomic rna	26
immune response to	26
the same as	26
the structural proteins	26
viral replication and	26
hodgson et al	26
allantoic fluid of	26
region in the	26
of a qx	26
did not show	26
inoculated into the	26
to study the	26
that the recombinant	26
could not be	26
cleavage site of	26
time taqman rt	26
of turkey coronavirus	26
processing of the	26
no conflict of	26
a novel variant	26
the extent of	26
vaccination against avian	26
have been used	26
new genotype of	25
multiplicity of infection	25
strains were isolated	25
expressing a heterologous	25
birds in each	25
h strain of	25
confirmed that the	25
and tissue tropism	25
hewson et al	25
virus strain a	25
by using a	25
and incubated at	25
major structural proteins	25
analyses of the	25
the rescued virus	25
holmes et al	25
analysis of avian	25
results indicate that	25
rottier et al	25
live attenuated ibv	25
the result of	25
transmissible gastroenteritis coronavirus	25
only in the	25
ibv m protein	25
days after challenge	25
chinese ibv isolates	25
a novel coronavirus	25
the ibv spike	25
occurred in the	25
associated with a	25
only a few	25
collected from the	25
of the pathogenic	25
development and use	25
of live attenuated	25
to amplify the	25
of the leader	25
restriction fragment length	25
genbank accession number	25
in embryonated chicken	25
of the genomic	25
thirds of the	25
and phylogenetic analysis	25
those in the	25
between p and	25
washed three times	25
like to thank	25
the most important	25
virus resulting from	25
protein genes of	25
calculated using the	25
ibv ck ch	25
lesions were observed	25
coronavirus in china	25
epitopes on the	25
of natural recombination	25
ibv s protein	25
were inoculated into	25
a n a	25
commercial broiler chickens	25
with the exception	25
heterologous spike gene	25
ibv s gene	25
a heterologous spike	25
of the human	25
was similar to	25
ch lsd i	25
is unable to	25
ch lhlj vii	25
demonstrate that the	25
presence of a	25
sequences of s	25
analysis based on	25
n protein in	25
comparative analysis of	25
this suggests that	25
on the basis	25
negative control group	25
and n gene	25
appear to be	25
and newcastle disease	25
been reported to	25
in the two	25
were collected and	25
appeared to be	25
third of the	25
and other birds	25
a final extension	25
of the mass	25
and it is	25
ibv e and	25
the efficacy of	25
followed by a	25
in agreement with	24
protection induced by	24
effect of the	24
some of these	24
coronavirus spike proteins	24
for the ibv	24
allantoic fluids were	24
genetics system for	24
is known to	24
in great britain	24
mutant s protein	24
in the rhbm	24
the ibv isolates	24
the ibv e	24
the site of	24
the s genes	24
the isolate sc	24
the structural and	24
fang et al	24
s proteins of	24
isolation and characterization	24
tissue tropism and	24
amino acids in	24
the membrane was	24
was incubated with	24
a series of	24
which is a	24
on vero cells	24
with respect to	24
an attempt to	24
the virus to	24
the protective efficacy	24
of the mhv	24
that have been	24
virus with a	24
replicase gene of	24
in the golgi	24
via the allantoic	24
required for the	24
a and orf	24
into the allantoic	24
n a n	24
recent years in	24
in the formation	24
for the avian	24
different from the	24
gorbalenya et al	24
viruses isolated from	24
the exception of	24
callison et al	24
is mediated by	24
known to be	24
positively selected sites	24
from infected chickens	24
genotypes of ibv	24
and of the	24
as per the	24
coronaviruses in poultry	24
ch ljl strain	24
to compare the	24
and that the	24
to determine if	24
in this report	24
and sequencing of	24
the differences in	24
with use of	24
be related to	24
cd t lymphocytes	24
innate immune responses	24
hepatitis c virus	24
ibv strain m	24
response to ibv	24
the spread of	24
cytotoxic t lymphocytes	24
expressing the s	24
the m and	24
chicken farms in	24
and stained with	24
reported that the	24
evolution of ibv	24
c for h	24
portion of the	24
was inoculated into	24
ibv infected cells	24
incubated for h	24
properties of the	23
vaccinia virus genome	23
when compared with	23
the s domain	23
bronchitis virus molecular	23
of hy on	23
performed with the	23
in broiler chickens	23
identity with the	23
summarized in table	23
was also observed	23
the relationship between	23
used to amplify	23
ibv strains have	23
which has been	23
humoral and cellular	23
virulent ibv strain	23
cells expressing the	23
of the attenuated	23
and the other	23
this study are	23
n gene was	23
more than one	23
ml of the	23
we did not	23
wickramasinghe et al	23
a and ab	23
disease of chickens	23
presence or absence	23
were shown to	23
protection against ibv	23
the mrna expression	23
infectious peritonitis virus	23
the nrtw i	23
is shown in	23
in viral replication	23
the distribution of	23
df cells were	23
against ibv infection	23
induced by ibv	23
as a negative	23
genotype infectious bronchitis	23
is important for	23
is not known	23
ibv reference strains	23
were incubated for	23
of the nucleotide	23
evidence of recombination	23
of ibv s	23
were maintained in	23
infection with ibv	23
used to determine	23
can be seen	23
of enteric viruses	23
infected cells and	23
lee et al	23
levels in the	23
trees were constructed	23
of each primer	23
the cleavage site	23
calculated according to	23
infected with wild	23
were similar to	23
of transmissible gastroenteritis	23
location of the	23
ibv strains from	23
of the family	23
ibv vaccine strains	23
in the early	23
the existence of	23
bronchitis virus coronavirus	23
evolution of the	23
mutation in the	23
in chicken embryos	23
study of the	23
the entire s	23
in vitro assembly	23
the causative agent	23
infected with a	23
or absence of	23
the severe acute	23
the arkga p	23
the introduction of	23
thermo fisher scientific	22
of the rescued	22
for s and	22
is thought to	22
and differentiation of	22
molecular epizootiology of	22
of the world	22
dhinakar raj jones	22
variant infectious bronchitis	22
and the s	22
in the region	22
the interaction between	22
ibv field isolates	22
in chickens infected	22
of the vaccine	22
at days after	22
the age of	22
bind to the	22
analysis of s	22
with the spike	22
and compared with	22
were cultured in	22
gene and the	22
rndv expressing s	22
by the coronavirus	22
in viral rna	22
spike glycopolypeptide s	22
and in vitro	22
replication of ibv	22
emergence of novel	22
and cloacal swabs	22
replicate in the	22
is necessary to	22
spf chicken embryos	22
is able to	22
evidence of natural	22
of new ibv	22
infectious laryngotracheitis virus	22
amount of virus	22
were fixed and	22
the golgi apparatus	22
eid of the	22
s protein and	22
be used for	22
immunity in chickens	22
expressing s protein	22
resulting in the	22
a significant difference	22
gene demonstrates that	22
are essential for	22
the sensitivity of	22
phylogenetic trees were	22
the di rna	22
the pathogenic m	22
linked sialic acid	22
protection conferred by	22
serotypes of ibv	22
the long view	22
structural and functional	22
spike gene demonstrates	22
it appears that	22
isolated from a	22
has been used	22
of rndv expressing	22
no clinical signs	22
for viral replication	22
expression of nsp	22
have been isolated	22
the localization of	22
for infectious bronchitis	22
the results were	22
i and ck	22
the helicase protein	22
by using the	22
m and s	22
it was found	22
and determination of	22
ldl i strain	22
recombinant ibvs and	22
function of the	22
cells treated with	22
the cek cells	22
attenuated ibv vaccine	22
seen in the	22
of avian influenza	22
it is a	22
observed in cells	22
vaccinated with the	22
influenza a viruses	22
of coronavirus ibv	22
in wild birds	22
beaudette strain of	22
virus replication and	22
corse and machamer	22
at dpi in	22
antigenic domains on	22
that it is	22
causative agent of	22
variations in the	22
the deduced amino	22
sites of the	22
resulting in a	22
as described in	22
to analyze the	22
and the ck	22
ibv have been	22
to the vaccine	22
infection of the	22
serotypes and genotypes	22
virus from infected	22
bronchitis virus field	22
of ibv from	22
the rest of	22
at h postinfection	21
the chickens in	21
was performed to	21
in the oviduct	21
the partial s	21
alignment of the	21
were detected by	21
novel genotype of	21
by homologous recombination	21
detection of antibodies	21
aiv and ibv	21
veterinary research institute	21
ibv infection and	21
groups of chickens	21
study showed that	21
contrast to the	21
in a previous	21
to harmonize virus	21
at and h	21
the potential to	21
has been described	21
circulating in vaccinated	21
relationship between the	21
for each virus	21
belong to the	21
from the netherlands	21
with regard to	21
genotype of infectious	21
system for the	21
difference between the	21
with the viral	21
of antigenic variants	21
e l and	21
cells were harvested	21
it is also	21
and s genes	21
years of infectious	21
attempt to harmonize	21
losses in the	21
was not detected	21
h of storage	21
interaction between the	21
can be found	21
vaccination against infectious	21
of orf a	21
the other two	21
challenge with infectious	21
have demonstrated that	21
ibv isolates from	21
porcine epidemic diarrhea	21
followed by the	21
genetic diversity and	21
n protein and	21
the tracheas of	21
pcr product was	21
and rh scs	21
the presence or	21
to the virus	21
phylogeny of infectious	21
from the other	21
phylogenetic tree was	21
bronchitis virus serotypes	21
ibv is a	21
recombination events in	21
vennema et al	21
the coronaviridae family	21
and cloned into	21
region of s	21
that there were	21
attenuated live vaccines	21
of the sars	21
in the phylogenetic	21
viral replication in	21
incubated for min	21
innate and adaptive	21
harmonize virus classification	21
the regulation of	21
identification and characterization	21
to reduce the	21
virus as a	21
genotype of nephropathogenic	21
and immunogenicity of	21
not yet been	21
and emergence of	21
and h post	21
of infection in	21
infectious bronchitis research	21
of h n	21
samples were positive	21
is dependent on	21
epidemic diarrhea virus	21
s or s	21
ibv field strains	21
the eggs were	21
detection of avian	21
s spike glycoprotein	21
have been developed	21
viral rna in	21
the human coronavirus	21
and vaccine strains	21
ibv and mhv	21
the recovery of	21
used to identify	21
the e and	21
were compared with	21
based phylogeny of	21
makino et al	21
was amplified by	21
infected cells were	21
there is no	21
and the virus	21
in chickens and	21
and cd t	21
was identified in	21
the degree of	21
h n influenza	21
and used for	21
the infected chickens	21
m proteins of	21
detection of a	21
the recombinant ibv	21
blot analysis of	21
serve as a	21
it can be	21
the ratio of	20
the supernatant was	20
may play a	20
for virus isolation	20
coding region of	20
genome of ibv	20
no competing interests	20
growth kinetics of	20
results indicated that	20
transfected with the	20
with biological functions	20
was obtained from	20
the ibv cdna	20
mucus in the	20
determinant of pathogenicity	20
consistent with previous	20
randomly divided into	20
on the cell	20
to the er	20
by reverse transcription	20
ibv h bi	20
of the cells	20
isolates in china	20
is an important	20
of this virus	20
the phylogenetic trees	20
amplification of the	20
of antibodies against	20
located at the	20
the recombinant s	20
highly significant at	20
isolated in japan	20
that in the	20
bronchitis virus date	20
virus is a	20
of the strain	20
of virus replication	20
to interact with	20
a laboratory manual	20
was performed on	20
of ibv genome	20
determined by the	20
qx strain of	20
amino acid identities	20
deletions in the	20
kusters et al	20
three different strains	20
after infection with	20
in the infected	20
nature of the	20
an avian coronavirus	20
the ibv infection	20
to the host	20
to replicate in	20
the assembly of	20
isolates of infectious	20
correlation with biological	20
it is necessary	20
and accessory genes	20
of genetic diversity	20
the growth of	20
control of ibv	20
tropism and pathogenicity	20
the tracheal epithelium	20
using the neighbor	20
of veterinary medicine	20
avian influenza viruses	20
antigenic variants of	20
glycoprotein of ibv	20
detection and differentiation	20
genes of ibv	20
the ts mutant	20
chicken embryonated eggs	20
infectious bronchitis vaccine	20
evaluation of protection	20
were aligned using	20
the subgenomic mrnas	20
cleavage recognition site	20
products were purified	20
proteins involved in	20
ziebuhr et al	20
a dose of	20
a recent study	20
chickens challenged with	20
control of infectious	20
as a consequence	20
by avian infectious	20
ibv beaudette strain	20
were immunized with	20
well plates were	20
in the order	20
percentages of cd	20
binds to the	20
of subgenomic mrnas	20
virus isolation and	20
role in viral	20
was used in	20
amplified by rt	20
amino acids within	20
were isolated in	20
in the cells	20
was found that	20
of cd and	20
in china between	20
relative mrna expression	20
of ibv e	20
of murine coronaviruses	20
localization of the	20
ck sudan ar	20
virus isolation in	20
a functional receptor	20
spike gene of	20
taq dna polymerase	20
c and d	20
pathogenicity of ibv	20
of ibv have	20
the orf b	20
from that of	20
under the control	20
between the s	20
to be an	20
carried out by	20
infected vero cells	20
vaccine strains and	20
strains used in	20
domains on the	20
genotypes based on	20
central nervous system	20
ibv strains are	20
ibv strains that	20
zhou et al	20
nrtw i strain	20
the innate immune	20
chickens in each	20
ibv vaccine strain	20
virulent ibv challenge	20
efficacy of an	20
of ibv has	20
reaction was carried	20
mediated by the	20
control of ib	19
added to each	19
was calculated using	19
ibv h strain	19
of infectious virus	19
isolation and molecular	19
is likely to	19
different regions of	19
nucleocapsid protein and	19
trachea and kidneys	19
of the in	19
to control the	19
of new variants	19
is not a	19
vero cells and	19
a multiplicity of	19
allantoic cavity of	19
eggs were incubated	19
spike glycoprotein of	19
in relation to	19
sense rna genome	19
the shell gland	19
mapping of the	19
the online version	19
been found to	19
a phylogenetic tree	19
h at room	19
crystal structure of	19
efficacy of rndv	19
a high degree	19
the diagnosis of	19
except for the	19
were performed in	19
were washed with	19
pathogenic m strain	19
m and e	19
at dpi and	19
s gene from	19
for the control	19
with or without	19
by flow cytometry	19
ibv and tcov	19
the significance of	19
isolate and the	19
and amino acids	19
positive and negative	19
be able to	19
in the netherlands	19
and inactivated vaccines	19
the spike precursor	19
can result in	19
complete protection against	19
are responsible for	19
bursa of fabricius	19
and western blot	19
due to a	19
the sequences were	19
load in the	19
sialic acid binding	19
genomic sequences of	19
at least three	19
rna was detected	19
of the major	19
isolates of ibv	19
in spite of	19
of the genes	19
to the nucleolus	19
virus in taiwan	19
gene in the	19
from recombination events	19
of coronavirus infectious	19
positive in the	19
isolation of a	19
the trachea of	19
accession numbers are	19
in the er	19
may have been	19
also showed that	19
genetic characterization of	19
studies of the	19
nsp and nsp	19
plays a role	19
were stained with	19
after challenge with	19
with ibv strain	19
to detect ibv	19
were determined using	19
be explained by	19
of vero cells	19
not result in	19
the finding that	19
of reed and	19
differences between the	19
from recombination among	19
protection of chickens	19
lower than that	19
a concentration of	19
a range of	19
sequences of ibv	19
purified using the	19
ibv and ibdv	19
detected in cells	19
to each well	19
the possibility of	19
majority of the	19
wege et al	19
were classified into	19
ibv replication in	19
to play a	19
in commercial poultry	19
of sars coronavirus	19
at a multiplicity	19
science and technology	19
youn et al	19
of the host	19
currently circulating in	19
old specific pathogen	19
virus s gene	19
was mixed with	19
of chickens infected	19
upper respiratory tract	19
of coronaviruses in	19
very similar to	19
the virus is	19
virus isolates in	19
s gene is	19
the functions of	19
type of ibv	19
was present in	19
results in the	19
this type of	19
and field strains	19
of the replicase	19
protective immune response	19
prevention and control	19
shown in figure	19
using a commercial	19
but not in	19
in ml of	19
is based on	19
the activity of	19
a b polyprotein	19
are presented in	19
is composed of	19
method of reed	19
monoclonal antibodies to	19
is difficult to	19
a mixture of	19
with the s	19
of the beaudette	19
was performed as	19
in chicken flocks	19
isolated from broiler	19
were obtained by	19
in the usa	19
the numbers of	19
partial s gene	19
reaction and restriction	19
was conducted using	19
immunodeficiency virus type	19
of avian pathogens	19
bronchitis virus genotypes	19
that there is	19
for min in	19
to the cell	18
at each time	18
with avian infectious	18
poultry farms in	18
immune responses and	18
essential for the	18
sybr green i	18
n and rhbm	18
for use in	18
no significant differences	18
was first reported	18
type of infectious	18
the differences between	18
ibvs and beaudette	18
novel nephropathogenic infectious	18
used in china	18
for the s	18
approved the final	18
a final volume	18
type infectious bronchitis	18
eggs were inoculated	18
fusion of the	18
based on s	18
was analyzed by	18
influenza h n	18
is caused by	18
of the membrane	18
of rna recombination	18
old embryonated chicken	18
associated with neutralization	18
titer of ibv	18
of recombination events	18
l and e	18
signs were observed	18
tract of chickens	18
were propagated in	18
a new variant	18
the a and	18
vaccinated flocks in	18
have no competing	18
mrna expression of	18
gene encoding the	18
luytjes et al	18
a nephropathogenic strain	18
in tracheal samples	18
swabs were collected	18
forward and reverse	18
with neutralization epitopes	18
e l glycoprotein	18
and development of	18
and membrane proteins	18
formation of the	18
strain of the	18
it is the	18
netherlands as a	18
the relative mrna	18
of the pathogenicity	18
three infectious bronchitis	18
the difference in	18
control of the	18
not required for	18
acids within hypervariable	18
use of a	18
virus around the	18
the allantoic fluids	18
was incubated at	18
of the present	18
jn sweden jn	18
ibv has a	18
with ibv at	18
rna genome of	18
of virus in	18
also observed in	18
is similar to	18
in commercial broiler	18
for each sample	18
from the same	18
in the cell	18
isolates from the	18
in the future	18
for rna recombination	18
among three different	18
the aim of	18
to the genbank	18
were randomly divided	18
from allantoic fluid	18
of protection induced	18
the pcr product	18
with the other	18
woo et al	18
sweden jn sweden	18
of the genus	18
as a potential	18
susceptible and resistant	18
responses induced by	18
coronaviruses have been	18
recombination among three	18
an outbreak of	18
glycoprotein are associated	18
reported to be	18
spike glycoprotein are	18
the isolation of	18
by virus isolation	18
sequence in the	18
constructed using the	18
the appearance of	18
variant of avian	18
immunized with rndv	18
t lymphocytes are	18
severe economic losses	18
passage in embryonated	18
and control of	18
from the ibv	18
of ib in	18
also known as	18
illustrated in fig	18
of the isolates	18
tracheal swab samples	18
of the rh	18
was consistent with	18
amino acid substitution	18
attenuated and inactivated	18
isolates from to	18
to the viral	18
protein was detected	18
by centrifugation at	18
b of the	18
generation of recombinant	18
dq ck ch	18
may not be	18
were harvested at	18
arg dq ck	18
were observed for	18
at least in	18
of the p	18
the netherlands as	18
complete genomic sequence	18
in mammalian cells	18
boursnell et al	18
not detected in	18
structural proteins and	18
phylogenetic analysis based	18
for ibv and	18
the primers used	18
the vaccine strains	18
which can be	18
korean ibv strains	18
has been found	18
western blot with	18
serial passage in	18
fused to the	18
proteins in the	18
its role in	18
is a member	18
was closely related	18
has been detected	18
zhao et al	18
of viral proteins	18
is a receptor	18
in ovo cpg	18
more closely related	18
of a virus	18
were prepared from	18
the genomes of	18
in vivo and	18
with ck ch	18
in western europe	18
of viral replication	18
was propagated in	18
the identity of	18
vaccines have been	18
rna replication and	18
of the experiment	18
s gene were	18
murine coronavirus spike	18
and approved the	18
within hypervariable region	18
were determined by	18
of the total	18
it may be	18
and other coronaviruses	18
virus genotypes in	18
the attenuated viruses	18
during the first	18
in young chickens	18
was observed between	18
to each other	18
efficacy of vaccination	18
of all the	18
rest of the	18
final volume of	18
viral replication cycle	18
and gross lesions	17
described in the	17
host response to	17
lai et al	17
regions in the	17
among ibv strains	17
closely related serotypes	17
bronchitis virus with	17
and housed in	17
rna and the	17
in the allantoic	17
old embryonated spf	17
may lead to	17
was detected using	17
order to determine	17
strain and the	17
against the ibv	17
washed with pbs	17
a receptor determinant	17
which may be	17
ibv and the	17
the detection and	17
use of live	17
were resuspended in	17
of the samples	17
virus in specific	17
and pathogenesis of	17
large number of	17
pathogenicity of the	17
of the entire	17
cavanagh and gelb	17
recombination detection program	17
the experiment was	17
genetic analysis of	17
were examined for	17
ibv strain h	17
ibv b isolate	17
to form the	17
were labeled with	17
infectious bronchitis vaccines	17
pcr was conducted	17
in the shell	17
han et al	17
that had been	17
identification of two	17
bronchitis coronavirus strains	17
s protein genes	17
the sialic acid	17
introduction of the	17
live infectious bronchitis	17
titers of the	17
in blocking buffer	17
need to be	17
determined to be	17
type and mutant	17
the nucleolus is	17
with rndv expressing	17
rna extracted from	17
protein and the	17
functional characterization of	17
cells were grown	17
immune response in	17
the expression level	17
for h and	17
a decrease in	17
of cells expressing	17
it should be	17
strain remains attenuated	17
but induces protective	17
jx kj gx	17
expressed as the	17
infected cells was	17
pathogenic and attenuated	17
care and use	17
from the genbank	17
caused by a	17
little or no	17
molecular analysis of	17
to the mass	17
are required for	17
of the massachusetts	17
isolation of avian	17
the study of	17
in ibv infected	17
remains attenuated but	17
like proteinase and	17
length ibv cdna	17
were observed at	17
by the same	17
bronchitis virus isolate	17
in all the	17
explained by the	17
gelb et al	17
studies showed that	17
spike and membrane	17
induces protective immunity	17
enteric viruses in	17
kb in length	17
e protein is	17
and b of	17
was detected at	17
embryo infectious dose	17
the standard curve	17
ibv ab test	17
on the hypervariable	17
between s and	17
n protein gene	17
spike glycoprotein subunit	17
gene and gene	17
the nature of	17
the virus and	17
virus in egypt	17
our understanding of	17
review of infectious	17
type ibv strains	17
virus in poultry	17
limit of detection	17
the ee index	17
region of avian	17
were closely related	17
to be involved	17
the er and	17
feng et al	17
assembly of the	17
performed using a	17
the length of	17
of the subgenomic	17
recombinant n protein	17
acid changes in	17
attenuated infectious bronchitis	17
respiratory tract and	17
carried out with	17
commercial live attenuated	17
cloning and sequencing	17
madsen et al	17
origin of the	17
could be due	17
viruses have been	17
and deduced amino	17
jx jx jx	17
were vaccinated with	17
laboratory manual for	17
of each virus	17
from those of	17
the oviduct of	17
are summarized in	17
a new serotype	17
beaudette and m	17
in cells transfected	17
n and h	17
acid substitutions in	17
acid differences in	17
across the entire	17
rapid diagnosis of	17
mock infected cells	17
bronchitis virus around	17
is an enveloped	17
previous studies have	17
was lower than	17
occurs in the	17
cell lysates were	17
viral rna replication	17
of innate immune	17
five ibv isolates	17
challenged with the	17
with ibv strains	17
of the qx	17
sun et al	17
of protective immunity	17
ibv spike gene	17
a consequence of	17
with eid of	17
feline aminopeptidase n	17
of the manuscript	17
of m and	17
strains in china	17
all ibv strains	17
jx jx kj	17
attenuated but induces	17
and cellular immune	17
based on these	17
to generate a	17
was constructed using	17
rh and rh	17
influenza c virus	17
sturman and holmes	17
results in a	17
chickens of all	17
well as in	17
and subjected to	17
characterization of avian	17
the circulation of	17
gel extraction kit	17
by reverse genetics	17
can also be	17
nucleotide and deduced	17
different ibv serotypes	17
of a single	17
recombinant ibv n	17
as can be	17
in samples from	17
virus field isolates	17
inactivated ibv vaccine	17
to induce virus	17
been used to	17
one cycle of	17
an avian infectious	17
the recombinant n	17
infectious bronchitis and	17
were purified using	17
to understand the	17
the order nidovirales	17
natural recombination within	17
of all ages	17
of rna viruses	17
challenge with the	17
variant of ibv	17
those of other	17
as measured by	17
chimaeric s gene	17
was significantly higher	17
identity of the	17
and m genes	17
available in the	16
genomic rna and	16
and the results	16
the sars coronavirus	16
single amino acid	16
adaptive immune responses	16
and the extent	16
ts mutant s	16
is important to	16
of the primers	16
strain h was	16
are present in	16
taxonomy of viruses	16
were considered significant	16
proteolytic processing of	16
the b type	16
observed between the	16
safety and efficacy	16
rna recombination of	16
an acute and	16
the detection limit	16
s and rhbm	16
n group on	16
read and approved	16
better understanding of	16
used to construct	16
s m n	16
modification of the	16
bronchitis virus detection	16
could be used	16
fluid was harvested	16
the time of	16
generation of a	16
antigenic characteristics of	16
of the vaccinated	16
immunopathogenesis of infection	16
in the m	16
circulating ibv strains	16
were fixed in	16
first isolated in	16
were introduced into	16
virus is not	16
fixed and labelled	16
infected with infectious	16
divided into three	16
of the core	16
to the control	16
birds in group	16
in association with	16
china in recent	16
highly pathogenic avian	16
sequence comparison of	16
by the virus	16
were conducted in	16
m protein is	16
in chicken embryo	16
stern and kennedy	16
the allantoic cavities	16
in ck cells	16
the ibv vaccine	16
was extracted using	16
in our laboratory	16
virus and other	16
to better understand	16
human coronavirus oc	16
hypericum perforatum l	16
during ibv infection	16
was detected with	16
the mutant virus	16
were also observed	16
also detected in	16
the tissue tropism	16
the e gene	16
mutation and recombination	16
may result in	16
a panel of	16
was based on	16
bronchitis virus c	16
the release of	16
of human coronavirus	16
total number of	16
induced by a	16
to infection with	16
huang et al	16
ibv e protein	16
the pcr reaction	16
high frequency of	16
polish ibv strains	16
especially in the	16
coronavirus n protein	16
can lead to	16
are expressed as	16
shown in the	16
sensitivity of the	16
in the group	16
at a concentration	16
s spike protein	16
was cloned into	16
using the same	16
of ibv variants	16
united states and	16
in our previous	16
from to dpi	16
available in genbank	16
phylogenetic tree of	16
of the receptor	16
the authors would	16