trigram

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trigram	frequency
the presence of	486
as well as	419
in the presence	299
cd t cells	251
the absence of	240
influenza a virus	227
in the absence	206
hepatitis c virus	203
were infected with	196
of the viral	196
in this study	188
as previously described	186
type i ifn	182
the cells were	175
severe acute respiratory	174
we found that	173
the number of	170
the role of	168
acute respiratory syndrome	167
in order to	163
is required for	162
was used to	161
due to the	156
hek t cells	152
in addition to	151
cells were infected	147
the expression of	145
to determine the	143
a and b	141
analysis of the	140
respiratory syndrome coronavirus	137
according to the	137
been shown to	136
at room temperature	135
based on the	134
for min at	132
vesicular stomatitis virus	132
cd t cell	131
of the virus	130
plos pathog doi	129
t cells were	126
for h at	125
west nile virus	122
in response to	118
cells were transfected	117
to determine whether	117
one of the	116
in contrast to	116
were transfected with	116
the amount of	114
data not shown	112
activation of the	110
structure of the	109
were used to	108
members of the	108
respiratory syncytial virus	107
of type i	106
the induction of	106
in infected cells	104
an moi of	104
was performed using	104
as described previously	103
consistent with the	103
the majority of	100
at an moi	98
the development of	97
was determined by	97
fig a and	96
has been shown	96
compared to the	96
expression of the	95
were incubated with	95
the ability of	95
was added to	94
the plasma membrane	93
cells were incubated	93
a number of	92
the formation of	90
pla g a	90
type i interferon	90
cells infected with	89
as shown in	89
cells were washed	89
the cell surface	88
the effect of	87
a role in	87
b and c	86
each of the	86
the level of	85
observed in the	85
as described above	85
mice infected with	85
at uc for	84
region of the	84
is essential for	84
the importance of	83
involved in the	83
cells were fixed	83
s q y	83
was used as	81
the use of	80
cells in the	80
it has been	79
the fact that	79
the production of	79
well as the	79
end of the	78
data suggest that	78
in vitro and	78
crystal structure of	78
mouse hepatitis virus	78
of influenza a	78
were treated with	77
herpes simplex virus	76
the context of	76
hepatitis b virus	76
mhv h a	75
shown to be	75
we show that	75
it is possible	74
suggesting that the	73
cells were then	73
was observed in	73
western blot analysis	73
associated with the	72
of viral rna	71
the lack of	71
were determined by	71
in the context	71
the case of	70
as described in	70
s and s	70
in the same	70
shown in fig	69
in the case	69
the activation of	69
in the cns	68
to that of	68
the activity of	68
is possible that	67
added to the	67
role in the	67
the effects of	66
the replication of	66
d b np	66
role of the	65
fig b and	65
of the host	65
is consistent with	65
activity of the	64
present in the	64
detected in the	64
is associated with	64
were used for	64
the levels of	64
human immunodeficiency virus	64
innate immune response	63
were obtained from	63
presence of the	63
c and d	63
the end of	63
the evolution of	63
the structure of	63
results indicate that	62
d b npn	62
kindly provided by	62
responsible for the	62
total rna was	62
these data suggest	62
s b fig	62
influenza a viruses	61
of infected cells	61
resulted in a	60
h n influenza	60
by flow cytometry	60
the possibility that	60
by plaque assay	60
an increase in	60
in cell culture	60
suggest that the	60
the emergence of	60
the release of	60
high levels of	59
b npn a	59
h at uc	59
production of infectious	59
changes in the	58
and s fig	58
s a fig	58
showed that the	58
were performed in	58
was used for	58
as compared to	58
dependent rna polymerase	58
by western blot	57
t cells in	57
ns and ns	57
middle east respiratory	57
contribute to the	57
of influenza virus	57
viral rna synthesis	57
and characterization of	56
v p y	56
found that the	56
a total of	56
indicating that the	56
were incubated for	56
of the two	56
the viral rna	55
required for the	55
mutations in the	55
similar to the	55
cell lysates were	55
appears to be	55
infection in the	55
p y f	55
three independent experiments	55
to determine if	54
east respiratory syndrome	54
viral gene expression	54
used as a	54
have been shown	54
as a result	54
were maintained in	54
the addition of	54
the control of	54
the mechanism of	54
the university of	54
derived from the	53
the viral genome	53
wild type and	53
the extent of	53
p and p	53
a role for	53
of hepatitis c	53
was carried out	53
virus infection in	52
found to be	52
such as the	52
is involved in	52
central nervous system	52
ha and na	52
part of the	52
cells were lysed	52
in agreement with	52
to this end	51
was detected in	51
in comparison to	51
analysis was performed	51
remains to be	51
be involved in	51
rlr signaling proteins	51
the interaction between	51
referred to as	51
has been reported	51
sialic acid binding	50
at h post	50
the host cell	50
or absence of	50
of cd t	50
presence or absence	50
the presence or	50
it is not	50
in a cells	50
of the human	50
in the cytoplasm	50
balb c mice	50
were purchased from	49
on the other	49
infected with the	49
materials and methods	49
we observed a	49
a a a	49
relative to the	49
in the viral	49
are shown in	49
ev c atpase	49
h n virus	49
cells were treated	49
in human cells	49
indicate that the	48
characterization of the	48
the identification of	48
was performed as	48
to assess the	48
vitro and in	48
of viral replication	48
virus replication and	48
fig c and	48
were incubated at	48
hmpv f protein	48
in complex with	48
to wild type	47
the impact of	47
and cells were	47
the endoplasmic reticulum	47
atrh and atrh	47
an important role	47
innate immune responses	47
a result of	47
presence of a	47
of severe acute	46
a variety of	46
replication in the	46
and in vivo	46
found in the	46
of vesicular stomatitis	46
as determined by	46
of c atpase	46
results suggest that	46
been reported to	46
of three independent	46
is dependent on	46
of the n	46
with pfu of	46
the interaction of	46
of a novel	46
on the cell	46
the course of	46
the binding of	45
for minutes at	45
show that the	45
and incubated for	45
we demonstrate that	45
most of the	45
of the ifn	45
that of the	45
have been reported	45
increase in the	45
compared to wild	45
all of the	45
incubated at uc	45
of the p	45
and in the	45
the d b	45
we conclude that	44
r interaction sites	44
was able to	44
of l pro	44
when compared to	44
the ability to	44
fig and s	44
nile virus infection	43
at the indicated	43
the generation of	43
data indicate that	43
play a role	43
demonstrate that the	43
was found to	43
were analyzed by	43
the percentage of	43
the hmpv f	43
the inhibition of	43
washed twice with	43
regions of the	43
unfolded protein response	43
the other hand	43
this suggests that	43
figure s a	43
have shown that	43
with the exception	42
the type i	42
to interact with	42
likely to be	42
the accumulation of	42
care and use	42
r interaction site	42
hela cells were	42
of rna viruses	42
cells were seeded	42
activated protein kinase	42
similar to that	42
during wnv infection	41
in the lungs	41
and stained with	41
in cells infected	41
were observed in	41
multiplicity of infection	41
was removed and	41
in the brain	41
corresponding to the	41
rna was extracted	41
cells treated with	41
rna was isolated	41
of rnase l	41
shown in figure	41
a range of	41
it is also	41
compared to wt	41
the innate immune	41
the coiled coil	41
these results indicate	41
with ml of	41
were used as	41
have been described	41
type i ifns	41
is mediated by	40
replication of the	40
were added to	40
were inoculated with	40
after infection with	40
its ability to	40
understanding of the	40
in the lung	40
and incubated with	40
min at uc	40
was performed in	40
viral replication and	40
at the same	40
the frequency of	40
of the cell	40
were grown in	40
it is likely	40
used for the	40
were detected in	40
cells were harvested	40
of ded p	40
strand rna synthesis	40
final concentration of	40
of the protein	40
was shown to	40
is able to	40
used in this	39
t cell responses	39
was measured by	39
was associated with	39
were stained with	39
were collected at	39
to the cell	39
semliki forest virus	39
of the c	39
between the two	39
of the upr	39
has not been	39
are essential for	39
were carried out	39
be due to	39
of the rna	39
are required for	39
of sindbis virus	39
position of the	39
to be a	39
the exception of	39
of the most	39
closely related to	38
wild type mice	38
the regulation of	38
a subset of	38
function of the	38
specific cd t	38
our understanding of	38
incubated for h	38
did not affect	38
type i and	38
virus replication in	38
we hypothesized that	38
some of the	38
with or without	38
in the nucleus	38
used to determine	38
at uc in	38
is responsible for	37
the surface of	37
infected cells were	37
provided by dr	37
are involved in	37
fetal bovine serum	37
viral replication in	37
member of the	37
in mammalian cells	37
in ml of	37
viral rna was	37
a critical role	37
in fig a	37
the tombusvirus replicase	37
supernatants were collected	37
the h n	37
venezuelan equine encephalitis	37
experiments were performed	37
and stored at	37
is known to	37
there was no	37
of h n	37
of infectious virus	36
and analyzed by	36
has also been	36
for min and	36
were resuspended in	36
that can be	36
binding to the	36
but not in	36
of these viruses	36
the central nervous	36
were generated by	36
for detection of	36
t cell response	36
suggests that the	36
in terms of	36
mice were infected	36
the site of	36
of the sars	36
immunodeficiency virus type	36
contributes to the	36
the function of	36
is shown in	36
the crystal structure	36
performed as described	35
due to a	35
sequences of the	35
in which the	35
as a control	35
are representative of	35
these data indicate	35
was performed on	35
the efficiency of	35
a final concentration	35
lymphocytic choriomeningitis virus	35
important role in	35
by using the	35
the ppxy motif	35
rna replication and	35
are known to	35
of the genome	35
plays a role	35
npn a cd	35
dependent on the	35
characterization of a	35
mediated by the	35
identification of a	35
were washed twice	35
residues in the	35
of ebov gp	35
d and e	35
the proportion of	35
of the three	35
we used a	34
induction of the	34
cells transfected with	34
regulation of the	34
at h p	34
crystal structures of	34
in wt mice	34
the loss of	34
by centrifugation at	34
from the same	34
is important for	34
at the end	34
the assembly of	34
to identify the	34
of the s	34
the severe acute	34
contrast to the	34
related to the	34
is likely that	34
stability of the	34
in the liver	34
induction of ifn	34
in both the	34
by using a	34
the observation that	34
at day p	34
component of the	34
of rlr signaling	34
the unfolded protein	33
uc for min	33
cells were maintained	33
followed by a	33
may be a	33
we did not	33
in line with	33
following reovirus infection	33
the detection of	33
in the human	33
open reading frame	33
to investigate the	33
of pla g	33
were collected and	33
have not been	33
the distribution of	33
we used the	33
equine encephalitis virus	33
required for efficient	33
the ratio of	33
for viral replication	33
for at least	33
in s table	33
to be the	33
of west nile	33
amino acid sequence	33
inhibition of the	33
not required for	33
low levels of	33
results demonstrate that	33
figure b and	33
simplex virus type	33
the viral replication	33
not due to	33
of protein vi	33
expression of a	33
was performed with	33
wide range of	33
has been demonstrated	33
mutation in the	32
viral and host	32
bind to the	32
are likely to	32
could not be	32
was purchased from	32
to study the	32
downstream of the	32
gene expression in	32
assays were performed	32
min at room	32
after h of	32
infected cells and	32
in hela cells	32
in the s	32
is likely to	32
figure s b	32
room temperature for	32
components of the	32
results in a	32
there was a	32
type i interferons	32
of the influenza	32
washed with pbs	32
a wide range	32
the pathogenesis of	32
the lungs of	32
with the viral	31
mechanism by which	31
there is no	31
vero e cells	31
these results suggest	31
was incubated with	31
with pbs and	31
the time of	31
of ebola virus	31
and resuspended in	31
performed as previously	31
were washed with	31
mm tris ph	31
approved by the	31
mapk erk pathway	31
of viral proteins	31
d b v	31
in combination with	31
of the h	31
two independent experiments	31
a decrease in	31
were subjected to	31
gene expression and	31
total number of	31
binding of the	31
b v p	31
was determined using	30
and the viral	30
strand rna viruses	30
s c fig	30
is the first	30
led to a	30
determine whether the	30
results show that	30
differences in the	30
knockdown of vcp	30
in vitro transcribed	30
to be involved	30
a and c	30
been associated with	30
as a negative	30
was performed by	30
of virus replication	30
hmpv b f	30
titers in the	30
murine hepatitis virus	30
and transferred to	30
the stability of	30
uc for h	30
we observed that	30
interaction with the	30
subclinical in utero	30
and activation of	30
interaction site mutants	30
the total number	30
were harvested at	30
coronavirus spike protein	30
infected with mhv	30
cd and cd	30
the secretory pathway	30
the influenza virus	30
none of the	30
terminal domain of	30
the supernatant was	30
and that the	30
the synthesis of	30
the same time	30
were performed as	30
with respect to	29
any of the	29
titers were determined	29
a reduction in	29
a consequence of	29
the influenza a	29
determined by the	29
for h with	29
data demonstrate that	29
was also observed	29
we also observed	29
plasmacytoid dendritic cells	29
were found to	29
at least three	29
of hepatitis b	29
a cells were	29
a functional sbs	29
insights into the	29
were transferred to	29
were fixed and	29
pcr products were	29
have been identified	29
of the receptor	29
specificity of the	29
ded p is	29
for h and	29
can be used	29
in virus replication	29
of a single	29
domain of the	29
washed three times	29
of prp sc	29
the p p	29
was quantified by	29
indicated by the	29
described in the	29
many of the	29
binds to the	29
role for the	29
it should be	29
viral titers in	29
to examine the	29
consistent with our	29
in the cell	29
the host response	29
were fixed with	29
used to generate	29
we propose that	29
we have previously	29
the virus to	28
included in the	28
at the time	28
identified in the	28
plates were incubated	28
to generate a	28
is critical for	28
well plates were	28
that in the	28
and cd t	28
a series of	28
by addition of	28
of the spike	28
the immune response	28
ie and ie	28
h n viruses	28
used in the	28
were seeded in	28
viral rna replication	28
demonstrated that the	28
were normalized to	28
identification of the	28
b and s	28
we determined the	28
obtained from the	28
mutation of the	28
animal care and	28
was isolated from	28
of mouse hepatitis	28
assembly of the	28
of the sbs	28
sequence of the	28
there is a	28
to note that	28
results showed that	28
in accordance with	28
b and d	28
compared with the	28
the fraction of	28
immune response to	28
the immune system	28
of wild type	28
expression levels of	28
is due to	28
of the cells	28
t cells and	28
supplemented with fetal	27
of the a	27
insight into the	27
to the host	27
in mosquito cells	27
levels of viral	27
the length of	27
prior to the	27
c virus infection	27
to test this	27
than that of	27
to be determined	27
resulting in a	27
located in the	27
virus rna replication	27
the reassortant viruses	27
processing of the	27
consistent with previous	27
at least two	27
were collected from	27
leading to the	27
of virus particles	27
interacts with the	27
known to be	27
was replaced with	27
assay was performed	27
is not known	27
in the virus	27
appeared to be	27
carried out using	27
alb ts and	27
the brains of	27
were able to	27
in these cells	27
as measured by	27
viral genomic rna	27
effect on the	27
to the nucleus	27
in pbs and	27
acid binding site	27
open reading frames	27
indicated that the	27
mhc class i	27
data show that	27
the n module	27
the existence of	27
a significant increase	27
an essential role	27
the dynamics of	27
led to the	27
the wild type	27
structural and functional	27
were performed using	27
expression in the	27
revealed that the	27
m and m	27
fig d and	27
cells were inoculated	26
consistent with a	26
in viral replication	26
been implicated in	26
carried out in	26
at uc and	26
in the first	26
well as in	26
the most abundant	26
a negative control	26
interact with the	26
that ded p	26
been shown that	26
i ifn production	26
virus infection and	26
structures of the	26
viruses in the	26
cells were grown	26
in the host	26
virus and host	26
in c cells	26
are important for	26
fatty acid synthesis	26
significant increase in	26
the analysis of	26
importance of the	26
g for min	26
in the two	26
are listed in	26
not affect the	26
bushy stunt virus	26
significant reduction in	26
protein and the	26
and incubated at	26
at the plasma	26
the virus and	26
was confirmed by	26
a mouse model	26
we suggest that	26
interferon regulatory factor	26
the contribution of	26
their ability to	26
similar results were	26
agreement with the	26
are associated with	26
appear to be	26
determined by plaque	26
of the total	26
serve as a	26
at least in	26
tomato bushy stunt	26
the in vitro	26
would like to	26
that there is	26
may contribute to	26
that have been	26
to the viral	26
in figure a	26
by confocal microscopy	26
no effect on	26
reduction in the	26
a and s	25
recognition of the	25
h post infection	25
the npn a	25
in a humidified	25
a virus infection	25
h post transfection	25
b np cd	25
nature of the	25
addition to the	25
a fraction of	25
induction of type	25
the hypothesis that	25
for the virus	25
the infected cells	25
depending on the	25
prior to infection	25
influenza virus infection	25
be used to	25
followed by the	25
were analyzed using	25
interaction between the	25
the recruitment of	25
as a consequence	25
like to thank	25
may not be	25
a multiplicity of	25
it is important	25
ability of the	25
to those of	25
the requirement for	25
incubated for min	25
wt and ifit	25
explained by the	25
cells that were	25
fold increase in	25
these results demonstrate	25
u os cells	25
were harvested and	25
a gift from	25
the rate of	25
supplemented with fbs	25
the result of	25
reads mapping to	25
of innate immune	25
is important to	25
of the m	25
virus titers were	25
compared to control	25
in the control	25
are able to	25
together with the	25
by western blotting	25
s and l	25
the sensitivity of	25
difference in the	25
the abundance of	25
be able to	25
the results of	25
side of the	25
and wt mice	25
are consistent with	25
to the plasma	25
the respiratory tract	24
suggested that the	24
under the control	24
was assessed by	24
to play a	24
is thought to	24
its role in	24
stored at uc	24
length of the	24
morbidity and mortality	24
to wt mice	24
innate and adaptive	24
performed using the	24
at the cell	24
of ns and	24
of two independent	24
viral dna replication	24
in mdck cells	24
had no effect	24
is an important	24
to confirm that	24
at hours post	24
of a viral	24
the ebola virus	24
at the n	24
the ends of	24
cells per well	24
emerging infectious diseases	24
higher levels of	24
the notion that	24
use of the	24
of the four	24
h and h	24
of di particles	24
transfected with plasmids	24
in text s	24
i and iii	24
of chikungunya virus	24
close to the	24
to the virus	24
we compared the	24
could be detected	24
flock house virus	24
correlated with the	24
is necessary for	24
which in turn	24
d b gp	24
were found in	24
has been proposed	24
as part of	24
in a dose	24
was observed for	24
accordance with the	24
the possibility of	24
rna synthesis by	24
was not affected	24
structure of a	24
with the indicated	24
possible that the	24
days post infection	24
tumv intercellular movement	24
of mhv spike	24
did not show	24
as indicated by	23
we examined the	23
previously shown to	23
and inhibition of	23
incubated with the	23
and used to	23
shown in the	23
we tested whether	23
for hours at	23
in the regulation	23
were shown to	23
majority of the	23
portion of the	23
corresponds to the	23
comparison of the	23
the increase in	23
be explained by	23
consistent with this	23
as a template	23
of a new	23
the association of	23
the inhibitory effect	23
the mean of	23
h a replication	23
followed by incubation	23
and use committee	23
with fetal bovine	23
on the surface	23
c bl mice	23
indicated time points	23
been demonstrated to	23
sev cantell ld	23
with the same	23
in mice infected	23
virus in the	23
we were able	23
it may be	23
hepatitis virus strain	23
critical role in	23
of at least	23
were cultured in	23
of cells with	23
we sought to	23
of the reassortant	23
were used in	23
the capacity of	23
we demonstrated that	23
are shown as	23
data are presented	23
of the family	23
the involvement of	23
the in vivo	23
the indicated time	23
the viral life	23
bound to the	23
respiratory syndrome virus	23
the kinetics of	23
are thought to	23
c virus rna	23
leads to the	23
for the presence	23
the specificity of	23
can also be	23
incubation at uc	23
previous studies have	23
fmdv l pro	23
essential for the	23
are presented as	23
in the supernatant	23
viruses have been	23
likely due to	23
ebola virus glycoprotein	23
resulted in the	23
the viral capsid	23
in the gut	23
but not the	23
a significant reduction	23
in fig b	23
the position of	23
was generated by	23
and s b	23
these data demonstrate	23
thermo fisher scientific	23
or in the	23
to the same	23
was detected by	23
were unable to	23
the introduction of	23
for the development	23
recombinant ded p	23
stat and grb	23
mapped to the	23
by the addition	23
and use of	23
is capable of	23
were then washed	23
protein in the	23
a panel of	23
of a virus	23
by the viral	23
added to each	22
the cut site	22
cells were co	22
of the wt	22
upstream of the	22
using the same	22
the indicated times	22
to the n	22
at and hpi	22
that had been	22
was required for	22
inhibitory effect of	22
the basis of	22
site in the	22
in s fig	22
differences between the	22
in figure s	22
note that the	22
cells were cultured	22
for h before	22
single amino acid	22
in the n	22
and function of	22
the capacity to	22
the sars coronavirus	22
of the other	22
s fig and	22
tumor necrosis factor	22
in utero zikv	22
effect of the	22
interactions between the	22
of dengue virus	22
those of the	22
infection and the	22
the united states	22
in utero infection	22
of ptx in	22
to our knowledge	22
e and f	22
initial binding rate	22
in the pre	22
structural basis for	22
in pbs containing	22
resulting in the	22
transfected with a	22
in patients with	22
of mice infected	22
of the cellular	22
has been previously	22
were quantified by	22
of the complex	22
observed for the	22
of human cytomegalovirus	22
positive cells were	22
and stained for	22
leads to a	22
dmem supplemented with	22
cloned into the	22
possibility that the	22
are responsible for	22
had fiber head	22
antiviral activity of	22
in the infected	22
to a final	22
the hepatitis c	22
free virus particles	22
structure and function	22
p p modification	22
of the first	22
and the cells	22
to confirm the	22
rna synthesis in	22
plaque assay on	22
virus infection by	22
at the university	22
a cd t	22
was kindly provided	21
mouse model of	21
because of the	21
gut cd t	21
formation of the	21
control of the	21
the side chain	21
surface of the	21
viral life cycle	21
of viral dna	21
is required to	21
protein kinase c	21
associated with a	21
be noted that	21
utero zikv infection	21
hendra and nipah	21
viral and cellular	21
for min to	21
did not significantly	21
of rna synthesis	21
cells and the	21
no significant differences	21
and the virus	21
not appear to	21
absence of the	21
has been described	21
was subjected to	21
the sinv replicase	21
carried out as	21
virus nonstructural protein	21
and subjected to	21
results were obtained	21
complex with the	21
the degree of	21
capsid protein and	21
luciferase activity was	21
not yet been	21
wt d b	21
hours post infection	21
in this case	21
be important for	21
due to its	21
and of the	21
to test the	21
that the majority	21
of these proteins	21
linked to the	21
of cytokine signaling	21
normalized to the	21
amino acid residues	21
the onset of	21
not essential for	21
may also be	21
more than of	21
presented as mean	21
protease inhibitor cocktail	21
is not clear	21
human monoclonal antibody	21
the wt and	21
should be noted	21
stably transduced with	21
cells were collected	21
then incubated with	21
the sfv complex	21
cleavage of the	21
conformational changes in	21
rna in the	21
were lysed and	21
adaptive immune response	21
of infectious hcv	21
the diversity of	21
were infected at	21
that it is	21
a function of	21
encoded by the	21
more than one	21
large number of	21
at least one	21
the virus in	21
is similar to	21
early and late	21
were stimulated with	21
belonging to the	21
this is the	21
p replication protein	21
rna virus replication	21
was extracted from	21
a combination of	21
induction of socs	21
and atrh helicases	21
of template rnas	21
of viral particles	21
of the capsid	21
the crystal structures	21
sox cut sites	21
removal of the	21
the capsid protein	21
were obtained by	21
away from the	21
with the following	21
affected by the	21
for hr at	21
the interactions between	21
results in the	21
transfected with the	20
the genome of	20
of infectious particles	20
at a multiplicity	20
and did not	20
h ss n	20
of viral infection	20
which is a	20
amav and tcrv	20
were identified in	20
fixed and stained	20
an inhibitor of	20
of t cells	20
of a functional	20
was obtained from	20
of the ppxy	20
the conformation of	20
antibodies were used	20
sev cantell hd	20
fraction of the	20
three times with	20
from infected cells	20
used as the	20
ss n ps	20
c rasm a	20
compared to those	20
t cell receptor	20
twice with pbs	20
clarified by centrifugation	20
of the genomic	20
virus infected cells	20
was determined in	20
is a major	20
we would like	20
i and mda	20
reported to be	20
in human and	20
not affected by	20
the host immune	20
the interaction with	20
important for the	20
the significance of	20
two of the	20
the present study	20
the ma virus	20
to evaluate the	20
activity in the	20
is that the	20
protein is a	20
h after transfection	20
rosavirus c rasm	20
shown that the	20
required for viral	20
for virus replication	20
of the immune	20
of wt mice	20
is supported by	20
for analysis of	20
viral rna in	20
to cry b	20
replication and transcription	20
during the course	20
in viral rna	20
change in the	20
fold dilutions of	20
statistical analysis was	20
institutional animal care	20
the genomic rna	20
representation of the	20
and samd l	20
the nucleus and	20
with sev cantell	20
of the infection	20
the treatment of	20
in control cells	20
for min in	20
based on these	20
in cells expressing	20
of the interaction	20
in the formation	20
the distal ileum	20
fig e and	20
response to the	20
one of these	20
n influenza a	20
of the l	20
in a mouse	20
be detected in	20
by day p	20
as evidenced by	20
of human immunodeficiency	20
in viral load	20
was detected using	20
is the most	19
suppressor of cytokine	19
host response to	19
mpv and mpv	19
institutes of health	19
the limit of	19
of the type	19
were fixed in	19
for production of	19
was not detected	19
in the m	19
of host cell	19
pcr was performed	19
kshv lytic replication	19
effects of the	19
of sendai virus	19
rpm for min	19
n influenza virus	19
transfected cells were	19
rabbit polyclonal anti	19
during chronic hiv	19
as a model	19
ss h ps	19
hcv rna replication	19
to understand the	19
of rl mice	19
to form a	19
c and s	19
under these conditions	19
development of a	19
of the gp	19
s b table	19
wt and mutant	19
a member of	19
and evolution of	19
supporting information s	19
for the first	19
performed in triplicate	19
decrease in the	19
the infectivity of	19
amplification of the	19
entry of iav	19
replication in vitro	19
rna replication in	19
and h n	19
er and golgi	19
there is an	19
the entry of	19
the absence or	19
to measure the	19
in this region	19
heat shock protein	19
type and mutant	19
to further investigate	19
viruses such as	19
mechanisms by which	19
allowed us to	19
bovine serum albumin	19
the outcome of	19
proteins in the	19
template rnas by	19
of which are	19
the potential to	19
control and lipin	19
in the brains	19
and expression of	19
is known about	19
confirmed that the	19
reactivated for h	19
in the mouse	19
were detected by	19
japanese encephalitis virus	19
by the presence	19
cell lines were	19
levels of the	19
plays an important	19
to analyze the	19
and adaptive immune	19
studies have shown	19
were washed three	19
important to note	19
of laboratory animals	19
of the individual	19
of the same	19
of the coronavirus	19
than of the	19
in nonhuman primates	19
of the fusion	19
of the innate	19
cells with a	19
infected cells was	19
in influenza a	19
levels in the	19
figure a and	19
of the tombusvirus	19
was cloned into	19
immune responses to	19
rna synthesis and	19
we showed that	19
essential role in	19
with type i	19
and ifit mice	19
of total rna	19
supported by the	19
or presence of	19
some of these	19
feline infectious peritonitis	19
the degradation of	19
has previously been	19
in the respiratory	19
levels of ifn	19
of the replicase	19
were allowed to	19
to better understand	19
the samples were	19
h at room	19
of the core	19
were washed and	19
and loss of	19
the standard deviation	19
rosavirus b and	19
there are no	19
that expression of	19
order to determine	19
results indicated that	19
national institutes of	19
and the other	19
has been observed	19
fetal calf serum	19
an a footprint	19
wt and sting	19
the sequence of	19
n ss h	19
in association with	19
have also been	19
mice were euthanized	18
samples were then	18
in the number	18
absence of a	18
in mice and	18
of the lhr	18
be responsible for	18
in wild type	18
early during infection	18
and found that	18
gp on the	18
and respiratory syndrome	18
similar to those	18
uc in a	18
viral load in	18
activity of c	18
many of these	18
at day post	18
utilisation of template	18
gene expression by	18
the cns of	18
one or more	18
for each of	18
cells with the	18
a set of	18
did not observe	18
infected with a	18
of mhv h	18
interactions with the	18
the discovery of	18
the ns protein	18
during viral infection	18
cytokines and chemokines	18
to the cells	18
in a manner	18
host gene expression	18
the growth of	18
shows that the	18
levels of isg	18
were confirmed by	18
of the manuscript	18
porcine reproductive and	18
binding to p	18
on vero cells	18
during the early	18
cell leukemia virus	18
and la ts	18
the cleavage site	18
ml of pbs	18
which has been	18
side chain of	18
proteins involved in	18
of wt and	18
mice sacrificed on	18
release of infectious	18
but did not	18
disease control and	18
the golgi apparatus	18
extent of fusion	18
deisgylase dub activity	18
is the only	18
k and k	18
variation in the	18
of the er	18
in humans and	18
as compared with	18
were generated using	18
experiment was repeated	18
no significant difference	18
is an essential	18
the viral membrane	18
and can be	18
critical for the	18
was calculated as	18
course of infection	18
shown in s	18
are expressed as	18
to viral infection	18
proteins were detected	18
day post infection	18
we find that	18
we report that	18
these results are	18
control and prevention	18
need to be	18
hev and niv	18
of the genus	18
of viral mrna	18
of semliki forest	18
of listeria monocytogenes	18
figure s c	18
immune responses and	18
s in text	18
of ev c	18
infection with the	18
reproductive and respiratory	18
were then incubated	18
were performed with	18
the size of	18
sacrificed on day	18
stages of infection	18
and virus production	18
by the virus	18
of convalescent plasma	18
for hour at	18
the new world	18
conformation of the	18
roles in the	18
mapping to the	18
isolated from a	18
produced in the	18
the availability of	18
is present in	18
using flow cytometry	18
lower respiratory tract	18
based on a	18
context of the	18
are present in	18
min at rt	18
the f protein	18
positive and negative	18
of the major	18
and normalized to	18
of the gene	18
as demonstrated by	18
absence or presence	18
at the level	18
in a single	18
the mechanism by	18
not result in	18
infection of the	18
in the c	18
loss of the	18
by alphavirus replicases	18
this study was	18
ross river virus	18
the efficacy of	18
is sufficient to	18
that the presence	18
of the ha	18
the same as	18
early stages of	18
we performed a	18
we have identified	18
infection in vitro	17
the asymmetric unit	17
severe and non	17
green fluorescent protein	17
the coding region	17
a key role	17
observed that the	17
coding region of	17
for the care	17
lead to the	17
of viral and	17
that are not	17
also observed in	17
on the viral	17
due to an	17
translation of the	17
may play a	17
in hek t	17
to be important	17
and in vitro	17
primary and secondary	17
along with the	17
of the blv	17
the nature of	17
been observed in	17
in transfected cells	17
and d b	17
of the infected	17
b and b	17
the virus was	17
data are shown	17
relative to wt	17
induced by the	17
to define the	17
with the wt	17
not in the	17
performed in the	17
data were analyzed	17
major histocompatibility complex	17
in table s	17
not sufficient to	17
that l pro	17
and for the	17
determine if the	17
to a lesser	17
the tgev rbd	17
to generate the	17
likely that the	17
of the coiled	17
as assessed by	17
with previous studies	17
that the virus	17
the viral protein	17
of hsp isoforms	17
the sialic acid	17
the initiation of	17
it is clear	17
we measured the	17
medium was replaced	17
the cytoplasm of	17
with that of	17
the idea that	17
terminus of the	17
immune response in	17
this is in	17
by binding to	17
influenza virus hemagglutinin	17
activity of l	17
on the virus	17
the negative strand	17
on day post	17
the viral polymerase	17
the removal of	17
the average of	17
fixed with paraformaldehyde	17
were separated by	17
serial dilutions of	17
caused by the	17
cells were reactivated	17
exclude the possibility	17
data showed that	17
the veev genome	17
amino acid sequences	17
in this paper	17
the design of	17
of pfu cell	17
ppxy late domain	17
region in the	17
cleavage site in	17
the rest of	17
k b s	17
that they are	17
and used for	17
of sars coronavirus	17
blot analysis of	17
were tested for	17
media was removed	17
of the antiviral	17
an rna virus	17
this is a	17
may be due	17
rather than the	17
as a reference	17
l pro c	17
served as a	17
isolated from the	17
distribution of the	17
cells were stimulated	17
s d fig	17
of a host	17
igm and igg	17
in the early	17
as a function	17
we analyzed the	17
are grateful to	17
molecular basis of	17
to be more	17
infected mice were	17
viruses and their	17
line with the	17
for disease control	17
nsp and nsp	17
performed using a	17
a determinant of	17
supernatants were harvested	17
and has been	17
is also a	17
at a final	17
synthesis by the	17
evolution of the	17
in absence of	17
transiently transfected with	17
the first time	17
we assessed the	17
analyzed the data	17
used to detect	17
the inoculum was	17
c virus replication	17
mouth disease virus	17
predicted to be	17
sendai virus c	17
in the replication	17
a mixture of	17
was observed when	17
oas rnase l	17
to calculate the	17
were lysed in	17
mice compared to	17
of s and	17
by interacting with	17
of core protein	17
body weight loss	17
the appearance of	17
for sting in	17
the genital mucosa	17
a solution of	17
cell extracts were	17
secondary antibodies were	17
altered peptide ligand	17
been linked to	17
a significant decrease	17
virus ns protein	17
as described for	17
in the second	17
at the non	17
but does not	17
for viral rna	17
domains of the	17
and pathogenesis of	16
a group of	16
in the genome	16
of mg ml	16
as in the	16
of lymphocytic choriomeningitis	16
rna helicase and	16
collected from the	16
cells compared to	16
that the observed	16
of the template	16
conjugated secondary antibodies	16
extracted from the	16
of host factors	16
contributed to the	16
dna sensing by	16
at the highest	16
identified as a	16
in vitro transcription	16
a concentration of	16
cd ko cells	16
essential for viral	16
the spike protein	16
defective interfering particles	16
of the mean	16
in control and	16
collected at the	16
limit of detection	16
ability to cleave	16
infected wild type	16
ha na balance	16
strand rna virus	16
is one of	16
fold change in	16
from the cytoplasm	16
were washed in	16
is sensitive to	16
was not observed	16
infection in a	16
not observed in	16
elevated levels of	16
the e g	16
samples were collected	16
we were unable	16
the likelihood of	16
out as described	16
thought to be	16
in the sbs	16
functional analysis of	16
are the most	16
was generated from	16
ded p and	16
low level of	16
proteins have been	16
top of the	16
host immune response	16
s a and	16
of the sinv	16
c and tnf	16
test this hypothesis	16
to characterize the	16
humoral responses in	16
aspects of the	16
infection in vivo	16
the golgi complex	16
in all cases	16
antibodies specific for	16
the potential of	16
a functional receptor	16
although it is	16
are needed to	16
was performed at	16
h n and	16
were transiently transfected	16
is crucial for	16
on the basis	16
dn vps b	16
the plates were	16
we are grateful	16
response to infection	16
supplemented with fcs	16
of the mouse	16
did not have	16
viral load and	16
account for the	16
during hcmv infection	16
the case for	16
in vivo and	16
rnas by alphavirus	16
c atpase is	16
the form of	16
have been implicated	16
of the mhv	16
the amino acid	16
due to their	16
our results show	16
no evidence for	16
and it is	16
the secretion of	16
propose that the	16
in the er	16
to of the	16
by degrading cgas	16
the care and	16
times post infection	16
and rnase l	16
is based on	16
resuspended in ml	16
functional receptor for	16
was replaced by	16
for h in	16
receptor binding and	16
in support of	16
respiratory tract infections	16
has been suggested	16
t cell activation	16
in all three	16
to have a	16
increasing amounts of	16
the viral replicase	16
medium supplemented with	16
inab and ifuse	16
viral capsid protein	16
was isolated using	16
a humidified incubator	16
simian immunodeficiency virus	16
hours after infection	16
cleavage of rlr	16
infected with wild	16
result in the	16
of eif e	16
and analysis of	16
human and animal	16
in the culture	16
type and bid	16
to induce the	16
a b signaling	16
seems to be	16
of the t	16
confirm that the	16
of the murine	16
significant differences in	16
we investigated the	16
that did not	16
african green monkey	16
previously shown that	16
and s a	16
sd of three	16
er stress response	16
is the major	16
wt mice at	16
effects on the	16
is independent of	16
each time point	16
the p gene	16
host and viral	16
from different genera	16
fusion hmpv f	16
of defective interfering	16
cellular response to	16
were detected using	16
rift valley fever	16
resulted in an	16
were determined using	16
is not required	16
t cell recognition	16
the sox cut	16
times with pbs	16
s c table	16
cells were transiently	16
statistical significance was	16
provided by the	16
by incubation with	16
the roles of	16
that could be	15
of the second	15
of the negative	15
in situ hybridization	15
dbp p and	15
interacting with the	15
in dmem supplemented	15
for in vitro	15
before and after	15
in the p	15
moi of pfu	15
hours post transfection	15
the catalytic site	15
using graphpad prism	15
late time points	15
avian influenza a	15
were mixed with	15
and added to	15
it appears that	15
the viral genomic	15
been identified in	15
is determined by	15
the antiviral activity	15
stained with anti	15
been reported in	15
replication and pathogenesis	15
whole cell lysates	15
at this time	15
was due to	15
different time points	15
correlation between the	15
adaptive immune responses	15
c for min	15
have been observed	15
mice were inoculated	15
presence of fcs	15
in pbs for	15
role for sting	15
of the productive	15
evolutionary history of	15
depend on the	15
wrote the paper	15
in protein vi	15
in some cases	15
the cells with	15
evidence for a	15
in the current	15
dengue virus infection	15
mediated activation of	15
ns and or	15
location of the	15
activation of nf	15
the process of	15
t cells are	15
was allowed to	15
the sequences of	15
conceived and designed	15
of the nsp	15
centers for disease	15
evp and mvp	15
the adaptive immune	15
have demonstrated that	15
hematoxylin and eosin	15
representative of two	15
particles in the	15
infected with either	15
for the indicated	15
of the central	15
the location of	15
reduction in viral	15
set out to	15
of the chikv	15
to wnv infection	15
sensing by degrading	15
the cleavage of	15
significant differences between	15
the time course	15
by comparing the	15
temperature for h	15
the tip of	15
the most important	15
in this work	15
is in agreement	15
of the corresponding	15
and designed the	15
to each well	15
designed the experiments	15
of all the	15
conjugated goat anti	15
the s and	15
the suppression of	15
virulence gene expression	15
capsid protein vp	15
has been associated	15
of the ebola	15
the selection of	15
in innate immune	15
in samd l	15
are colored in	15
it is unclear	15
of neutralizing antibodies	15
viral entry and	15
the following day	15
part of a	15
and s c	15
half of the	15
virus fusion protein	15
as opposed to	15
levels of virus	15
a period of	15
transferred to a	15
genes involved in	15
difference between the	15
mode of action	15
antiviral cd t	15
a sucrose cushion	15
later time points	15
which is the	15
the influence of	15
to bind to	15
depends on the	15
was measured using	15
mouse samd l	15
buffer containing mm	15
the severity of	15
mh mn ss	15
not alter the	15
y f and	15
little is known	15
subjected to western	15
presence of ver	15
hsc and ihsp	15
correlate with the	15
and treated with	15
to replicate in	15
implicated in the	15
the active site	15
in any of	15
a single amino	15
a previous study	15
chikungunya virus inhibits	15
for helpful discussions	15
of the brain	15
capacity of the	15
formation of a	15
of the d	15
of virus infection	15
influenza virus replication	15
was added and	15
of the rbd	15
of the full	15
of the wild	15
between the ifn	15
is located in	15
to test whether	15
in the induction	15
to the presence	15
the inflammatory response	15
infected with pfu	15
infection with a	15
a comparison of	15
to the wt	15
overnight at uc	15
evidence for the	15
at uc with	15
leukemia virus type	15
is dispensable for	15
increase in viral	15
production of the	15
viral titers were	15
was not due	15
inhibits dna sensing	15
of progeny virions	15
flow cytometry analysis	15
the a footprint	15
least in part	15
the next day	15
acts as a	15
indicates that the	15
number of reads	15
in this context	15
our results indicate	15
as early as	15
cell monolayers were	15
in brains of	15
human respiratory syncytial	15
of the ns	15
was analyzed using	15
npn a peptide	15
a lesser extent	15
its interaction with	15
and or ns	15
were calculated using	15
to the development	15
is indicated by	15
in the rd	15
the concentration of	15
at a concentration	15
the early secretory	15
new england biolabs	15
viral protein synthesis	15
em structure of	15
the results showed	15
virus inhibits dna	15
mhc class ii	15
on ice for	15
intensity of the	15
sought to determine	15
for each virus	15
cells in a	15
plays a critical	15
then added to	15
in conjunction with	15
in fig c	15
is regulated by	15
were cloned into	15
in vero cells	15
of the hmpv	15
the difference in	15
in the different	15
was digested with	15
and s table	15
to inhibit the	15
of the mrna	15
representative of three	15
the receptor binding	15
of the catalytic	15
size of the	15
a density of	15
regions in the	15
this was not	15
we have shown	15
the phosphorylation of	15
rna polymerase ii	15
no difference in	15
we do not	14
t rna polymerase	14
this study we	14
of the cytoplasmic	14
in the cytosol	14
of herpes simplex	14
at a density	14
weight loss and	14
in pro cells	14
of infection in	14
the s segment	14
protein that is	14
the fusion protein	14
santa cruz biotechnology	14
other cell types	14
we tested the	14
and h post	14
structural analysis of	14
presence of an	14
of the mie	14
at the site	14
of the e	14
significant difference in	14
early stage of	14
to ensure that	14
gene expression was	14
in each of	14
of the different	14
that map to	14
findings suggest that	14
in vitro studies	14
was analyzed by	14
was present in	14
in influenza virus	14
density in the	14
be affected by	14
located at the	14
listed in s	14
of the structural	14
amplified by pcr	14
through er and	14
s e fig	14
upon infection with	14
did not result	14
to the right	14
a k a	14
they do not	14
the er stress	14
h of infection	14
i ifn receptor	14
activity towards k	14
a lack of	14
that interact with	14
b y f	14
correspond to the	14
lower than that	14
to investigate whether	14
number of viruses	14
of an a	14
for efficient replication	14
virus infection is	14
a large number	14
more likely to	14
the full length	14
side chains of	14
growth factor receptor	14
use of laboratory	14
the action of	14
the supernatant of	14
and standard deviation	14
ml of the	14
association of the	14
of a human	14
proteins that are	14
to be an	14
with severe acute	14
and hours post	14
impact on the	14
phase of the	14
during virus infection	14
residue in the	14
table s in	14
in trex bcbl	14
the mixing test	14
the env tmd	14
yet to be	14
of a cells	14
to type i	14
extents of fusion	14
comparative analysis of	14
as rdrp initiation	14
in the genital	14
bind to a	14
cell response via	14
combined with the	14
the human cytomegalovirus	14
were associated with	14
serological assays for	14
cellular and viral	14
with a single	14
new world bat	14
have been previously	14
are presented in	14
peptide ligand vaccination	14
the initial binding	14
valley fever virus	14
is clear that	14
t cells with	14
host factors in	14
we next determined	14
induction of apoptosis	14
btv ns m	14
into host cells	14
and the supernatant	14
in liquid nitrogen	14
that has been	14
fact that the	14
calculated using the	14
both in vitro	14
in tissue culture	14
human coronavirus e	14
were isolated from	14
replication in cell	14
in our study	14
ratio of the	14
quantification of the	14
n and n	14
cells were plated	14
in severe and	14
for expression of	14
infected with veev	14
of mapk erk	14
of the hsp	14
initiation factor in	14
efficiency of the	14
early secretory pathway	14
day after infection	14
virus infection of	14
incubated overnight at	14
would be expected	14
of the inhibitor	14
is not essential	14
mtase as rdrp	14
in the binding	14
infected at an	14
type and ips	14
d b y	14
recombinant mhv s	14
expression levels were	14
the antiviral response	14
e and e	14
in presence of	14
by the tombusvirus	14
all of which	14
a ratio of	14
standard deviation of	14
sites in the	14
added to a	14
calculated as the	14
the cell membrane	14
cells at uc	14
and mhv h	14
mediated restriction of	14
the mixture was	14
and infected with	14
luciferase activity in	14
this may be	14
of this study	14
expression of ifn	14
yeast dbp p	14
no evidence of	14
for binding to	14
of the tgev	14
of the trimer	14
of serological assays	14
amino acid substitution	14
compared to mock	14
mice immunized with	14
the origin of	14
higher level of	14
a dilution of	14
essential amino acids	14
play an important	14
been reported for	14
of samd l	14
factor in ns	14
footprint on the	14
the matrix protein	14
genomic rna and	14
by a single	14
by measuring the	14
all samples were	14
the wt virus	14
significant decrease in	14
to compare the	14
which is consistent	14
of the sox	14
our data suggest	14
result of the	14
performed the experiments	14
can interact with	14
of the peptide	14
means sd of	14
in spite of	14
after which the	14
our finding that	14
new insights into	14
could be a	14
level in the	14
detection of sars	14
tgev and prcv	14
in this report	14
small molecule inhibitors	14
or infected with	14
and incubated in	14
g and g	14
it would be	14
of the hemagglutinin	14
in at least	14
was amplified by	14
helicase activity of	14
assigned to the	14
viral rna and	14
in the present	14
back to the	14
responses in severe	14
the risk of	14
ends of the	14
t cells that	14
sodium dodecyl sulfate	14
proteins of the	14
infected with sev	14
were analyzed for	14
key role in	14
was not significantly	14