quadgram

This is a table of type quadgram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

quadgram	frequency
for the detection of	175
for rapid detection of	106
display the preprint in	88
granted medrxiv a license	88
has granted medrxiv a	88
who has granted medrxiv	88
license to display the	88
a license to display	88
medrxiv a license to	88
to display the preprint	88
severe acute respiratory syndrome	87
copyright holder for this	85
the copyright holder for	85
is the author funder	78
mediated isothermal amplification assay	73
mediated isothermal amplification method	72
mediated isothermal amplification for	69
not certified by peer	65
which was not certified	65
certified by peer review	65
was not certified by	65
acute respiratory syndrome coronavirus	57
mediated isothermal amplification of	54
isothermal amplification of dna	52
development and evaluation of	50
the preprint in perpetuity	50
holder for this preprint	47
the sensitivity of the	47
isothermal amplification method for	42
isothermal amplification assay for	42
loop mediated isothermal amplification	41
this preprint this version	39
for this preprint this	39
preprint this version posted	39
for this this version	38
holder for this this	38
this this version posted	38
of severe acute respiratory	37
method for rapid detection	36
the detection limit of	35
amplification method for rapid	34
and evaluation of a	34
the specificity of the	33
isothermal amplification for rapid	32
assay for the detection	31
amplification for rapid detection	31
for the diagnosis of	31
assay for detection of	30
in the presence of	30
reuse allowed without permission	29
no reuse allowed without	29
international license it is	28
is made available under	28
license it is made	28
made available under a	28
it is made available	28
preprint the copyright holder	27
this version posted november	27
detection of mycobacterium tuberculosis	27
has been shown to	26
detection and differentiation of	26
isothermal nucleic acid amplification	26
was found to be	26
r o o f	25
r n a l	25
detection limit of the	25
p r o o	25
j o u r	25
o u r n	25
u r n a	25
used in this study	25
n a l p	24
a l p r	24
evaluation of a novel	24
detection of novel coronavirus	24
sensitivity and specificity of	24
l p r e	24
a detection limit of	23
was added to the	23
detection of severe acute	23
time reverse transcription loop	23
middle east respiratory syndrome	23
for rapid diagnosis of	22
the limit of detection	22
mediated isothermal amplifi cation	22
of japanese encephalitis virus	22
magnetic bead rna extraction	22
by reverse transcription loop	22
the rapid detection of	21
isothermal amplification using loop	21
sensitivity of the rt	21
high sensitivity and specificity	21
rna was extracted from	21
rapid detection of severe	21
amplification using loop primers	21
the results of the	21
this version posted july	21
of a novel loop	21
as shown in fig	21
a reverse transcription loop	20
amplification assay for rapid	20
and rapid detection of	20
the performance of the	20
mediated isothermal amplification using	20
mediated isothermal amplification reaction	19
for the rapid detection	19
on the other hand	19
detection and quantification of	19
accelerated reaction by loop	19
assay for rapid detection	18
of reverse transcription loop	18
was approved by the	18
as shown in table	18
this version posted may	18
the detection of sars	18
at c for min	18
role of autophagy in	18
east respiratory syndrome coronavirus	18
followed by cycles of	18
detection of west nile	18
available under a is	17
derived from magnesium pyrophosphate	17
under a is the	17
reaction by turbidity derived	17
rapid and sensitive detection	17
a is the author	17
and sensitive detection of	17
for min in a	17
by turbidity derived from	17
in the present study	17
study was approved by	17
turbidity derived from magnesium	17
method for detection of	17
from magnesium pyrophosphate formation	17
developed in this study	17
rapid detection of sars	16
of the lamp assay	16
lamp primers were designed	16
by notomi et al	16
isothermal amplification reaction by	16
a set of four	16
is one of the	16
rapid detection of west	16
of the lamp reaction	16
can be used to	16
of herpes simplex virus	16
fold serial dilutions of	16
at a constant temperature	15
of viral nucleic acids	15
mediated isothermal amplification rapid	15
specificity of the rt	15
of sybr green i	15
limit of detection of	15
a simple and rapid	15
added to the reaction	15
and differentiation of dengue	15
of west nile virus	15
with a detection limit	14
carried out in a	14
direct detection of mycobacterium	14
the sensitivity and specificity	14
amplification reaction by turbidity	14
limit of the rt	14
results showed that the	14
on a agarose gel	14
of a reverse transcription	14
of mycobacterium tuberculosis complex	14
rapid detection and differentiation	14
by agarose gel electrophoresis	14
method for the detection	14
of dengue virus serotypes	14
regions on the target	14
amplification assay for the	14
strand displacement dna synthesis	14
postweaning multisystemic wasting syndrome	14
virus by reverse transcription	13
in the detection of	13
differentiation of dengue virus	13
this study was to	13
lamp assay for the	13
and respiratory syndrome virus	13
was carried out in	13
sensitive and rapid detection	13
detection of influenza a	13
serial dilutions of the	13
the detection rate of	13
and specificity of the	13
for direct detection of	13
was performed in a	13
isothermal amplification for direct	13
mediated isothermal amplification assays	13
amplification for direct detection	13
transcriptase polymerase chain reaction	13
the results showed that	13
without the need for	13
by the naked eye	13
samples with a ct	13
intracellulare in sputum samples	13
reverse transcription polymerase chain	12
on the basis of	12
can be used for	12
evaluation of reverse transcription	12
detection of zika virus	12
porcine reproductive and respiratory	12
transcription loop mediated isothermal	12
the causative agent of	12
simple visual detection of	12
rapid detection of the	12
reproductive and respiratory syndrome	12
of the target sequence	12
diagnosis of infectious diseases	12
distinct regions on the	12
the polymerase chain reaction	12
of bst dna polymerase	12
of this study was	12
detection of herpes simplex	12
one of the most	12
f and b primers	12
transcription polymerase chain reaction	12
targeting the n gene	12
to that of the	12
as shown in figure	12
and simple visual detection	12
reverse transcription loop mediated	12
of middle east respiratory	11
mediated isothermal amplification a	11
the authors declare no	11
for the detection and	11
is based on the	11
has been used for	11
the acute phase of	11
specificity and sensitivity of	11
sequences and simple visual	11
and visual detection of	11
stained with ethidium bromide	11
with high sensitivity and	11
in the case of	11
than that of the	11
of influenza a viruses	11
in a water bath	11
dna polymerase with strand	11
detection of middle east	11
in the copyright holder	11
for the development of	11
nucleic acid amplification method	11
development of a reverse	11
using loop primers loop	11
the role of autophagy	11
of the lamp method	11
isothermal amplification for the	11
of f and b	11
for min and then	11
preprint in the copyright	11
detection of japanese encephalitis	11
influenza a and b	11
cyp c g g	11
nucleic acid amplification tests	11
the study was approved	11
a new generation of	11
this version posted august	11
polymerase chain reaction for	11
rapid diagnosis of h	11
ns serotype specific rt	11
with strand displacement activity	11
the optimal reaction temperature	11
dengue virus serotypes by	11
for the lamp reaction	11
for rapid and real	11
for detection of the	11
the preprint in the	11
no conflict of interest	11
as well as the	11
amplification assay for detection	11
polymerase with strand displacement	11
in the united states	11
a water bath or	11
the lamp reaction was	11
assay for rapid and	10
were used for the	10
of nucleic acid amplification	10
gene and n gene	10
can be used as	10
reactions were carried out	10
for rapid detection and	10
amplification for detection of	10
swine transmissible gastroenteritis coronavirus	10
viral hemorrhagic septicaemia virus	10
and evaluation of reverse	10
set of four primers	10
infection by a loop	10
a total volume of	10
development of reverse transcription	10
the lamp assay was	10
epidermal growth factor receptor	10
detection of viral nucleic	10
detection and typing of	10
amplification for the detection	10
can be detected by	10
targeting the orf ab	10
amplification of dna loop	10
j virol methods doi	10
the sensitivity of rt	10
mediated isothermal amplification detection	10
are shown in table	10
for detection of influenza	10
was used as a	10
visual detection of products	10
at the end of	10
of newcastle disease virus	10
used to determine the	10
samples were collected from	10
positive and negative samples	10
gene sequences and simple	10
in the absence of	10
nucleic acid amplification test	10
were found to be	10
virus infection by a	10
lamp assay can be	10
isothermal amplification detection of	10
of gene sequences and	10
higher than that of	10
of loop mediated isothermal	10
to determine the optimal	10
as a template for	10
for disease control and	9
it is possible to	9
assay for the rapid	9
molecular detection of sars	9
the best of our	9
visual detection of novel	9
in the form of	9
avian influenza virus infection	9
h n and h	9
best of our knowledge	9
and backward inner primer	9
work was supported by	9
assay developed in this	9
were used as templates	9
primers f and b	9
products were detected by	9
the specificity and sensitivity	9
been shown to be	9
of feline infectious peritonitis	9
fip and bip primers	9
in the lamp assay	9
high specificity and sensitivity	9
of viral hemorrhagic septicaemia	9
were used to determine	9
igm and igg antibodies	9
as well as in	9
viral rna was extracted	9
may be due to	9
the end of the	9
n and h n	9
lamp assay for detection	9
rapid molecular detection of	9
h n avian influenza	9
the orf ab gene	9
amplification of nucleic acids	9
the mixture was incubated	9
time monitoring of the	9
repeats of the target	9
and specific detection of	9
a color change from	9
rapid detection and typing	9
sensitivity of the lamp	9
mediated isothermal amplification development	9
on the target dna	9
be used for the	9
to the best of	9
in the lamp reaction	9
in the reaction mixture	9
of porcine circovirus type	9
was used as the	9
the severe acute respiratory	9
reverse transcriptase polymerase chain	9
lamp assay and the	9
the sequences of the	9
porcine epidemic diarrhea virus	9
this work was supported	9
reaction for the detection	9
a rapid and simple	9
be used as a	9
was performed as described	9
positive in the rt	9
a limit of detection	9
was performed using the	9
isothermal amplification assays for	9
at room temperature for	9
inverted repeats of the	8
in this study is	8
with the naked eye	8
evaluation of a loop	8
was to develop a	8
were designed based on	8
pcr was performed using	8
in clinical diagnosis of	8
incubated for min at	8
the reaction was performed	8
for detection of human	8
based on the principle	8
from red to yellow	8
can be used in	8
authors declare no conflict	8
perspectives in clinical diagnosis	8
e region of hpv	8
assay development and evaluation	8
reaction was carried out	8
a novel nucleic acid	8
rapid and visual detection	8
bead rna extraction protocol	8
i influenza a b	8
development and application of	8
to the reaction tube	8
room temperature for min	8
study was to develop	8
the spread of the	8
virus serotypes by a	8
lamp was carried out	8
on the principle of	8
isothermal amplification method rapid	8
a novel reverse transcription	8
lamp primers targeting the	8
been used for the	8
virus rapid detection and	8
transcription loopmediated isothermal amplification	8
of human influenza a	8
by lamp and pcr	8
detection of nucleic acids	8
clinical diagnosis of infectious	8
and can be used	8
for pdm h n	8
during the acute phase	8
the high sensitivity of	8
to detect viral rna	8
using the primer explorer	8
nucleic acid amplification technologies	8
a dna polymerase with	8
a rapid and sensitive	8
in accordance with the	8
amplification and detection of	8
polymerase chain reaction and	8
isothermal amplification assay development	8
the copy number of	8
classical swine fever virus	8
lamp assay for detecting	8
were compared with those	8
a final volume of	8
the magnetic bead rna	8
sybr green i was	8
a set of six	8
c for min and	8
has been used to	8
with hydroxynaphthol blue dye	8
loopmediated isothermal amplification assay	8
in a total volume	8
strand displacement activity and	8
detection of human herpesvirus	8
the hbv lamp reaction	8
n avian influenza virus	8
in this study was	8
lamp assay was developed	8
for the identification of	8
and the results were	8
development of a loop	8
has been applied to	8
which was not peer	8
the primer explorer v	8
of ibv and ndv	8
detection of turkey coronavirus	8
of the je virus	8
lamp assay is a	8
declare no conflict of	8
serotypes by a real	8
set of six primers	8
disease control and prevention	8
for the presence of	8
mixture was incubated at	8
application of the rt	8
a wide range of	8
the amplified dna products	8
of hepatitis c virus	8
the lamp method is	7
as a positive control	7
detection limit of rt	7
the formation of a	7
the use of a	7
of the lamp primers	7
of fip and bip	7
using the qiaamp viral	7
results of the rt	7
in a single reaction	7
lamp for rapid diagnosis	7
lamp assay has been	7
each of f and	7
the lamp assay for	7
isothermal amplification for detection	7
in the development of	7
in tissues and feces	7
the detection of viral	7
the ethics committee of	7
loop mediated isothermal amplifi	7
of chikungunya virus by	7
automated centrifugal microfluidic system	7
approved by the ethics	7
novel reverse transcription loop	7
innovative gene amplification technique	7
two pairs of primers	7
respiratory syndrome coronavirus rapid	7
chain reaction for the	7
coronavirus using a reverse	7
isothermal amplification development of	7
have been developed to	7
has been widely used	7
when the template input	7
of h n avian	7
novel nucleic acid amplification	7
a pair of primers	7
by the use of	7
the same as that	7
the specificity of rt	7
isothermal amplification as a	7
been used to detect	7
under a author funder	7
with high specificity and	7
the development of a	7
same as that of	7
pcr was carried out	7
acid amplification technologies for	7
of the newly developed	7
products were analyzed by	7
the reaction can be	7
the detection of hpv	7
amplification of dna rapid	7
detection of human influenza	7
coronavirus rna in tissues	7
new generation of innovative	7
primer sets were designed	7
compared to the qpcr	7
lamp assay developed in	7
were carried out using	7
rna samples isolated from	7
diagnostic method for infectious	7
of innovative gene amplification	7
west nile virus rapid	7
detection of newcastle disease	7
rapid detection of porcine	7
detecting ibv and ndv	7
can be detected in	7
dna accelerated reaction by	7
of isothermal nucleic acid	7
by the ethics committee	7
by a reverse transcription	7
have been developed for	7
dependent isothermal dna amplification	7
loopmediated isothermal amplification method	7
reverse transcription loopmediated isothermal	7
isothermal amplification combined with	7
without the need of	7
detection of porcine parvovirus	7
used for the detection	7
results indicated that the	7
mediated isothermal amplification combined	7
rna in tissues and	7
the clinical diagnosis of	7
based detection of sars	7
rapid diagnosis of coronavirus	7
in a single tube	7
method for infectious diseases	7
compared with that of	7
of dna accelerated reaction	7
times more sensitive than	7
nucleic acid amplification using	7
can be found in	7
available under a author	7
generation of innovative gene	7
used as a template	7
nucleic acid amplification methods	7
detection of chikungunya virus	7
alere i influenza a	7
rapid detection of novel	7
and aldh glu lys	7
was supported by the	7
and west nile virus	7
a final concentration of	7
human immunodeficiency virus type	7
the most widely used	7
isothermal amplification rapid detection	7
the results of rt	7
turkey coronavirus rna in	7
could be used for	7
diagnosis of coronavirus sars	7
were used in this	7
the use of lamp	7
of turkey coronavirus rna	7
nile virus rapid detection	7
time detection of japanese	7
by polymerase chain reaction	7
h avian influenza virus	7
roles of autophagy in	7
after the onset of	7
of mthfr c t	7
the diagnosis of fip	7
detection of feline coronavirus	7
was performed using a	7
methods for detection of	7
amplification of dna accelerated	7
highly sensitive detection of	7
results were compared with	7
a constant temperature of	7
a member of the	7
visual detection of turkey	7
the reaction mixture was	7
primers were designed to	7
is shown in fig	7
of human herpesvirus dna	7
would like to thank	7
been widely used in	7
for detection of mycobacterium	7
of the y chromosome	7
for the n gene	7
nucleic acid sequence based	7
also been shown to	6
from patients with pneumonia	6
declare that they have	6
a serial dilution of	6
lamp was performed as	6
cyp c a a	6
the orf a and	6
for the clinical diagnosis	6
reaction was performed at	6
white spot syndrome virus	6
amplification technologies for point	6
to determine the sensitivity	6
this article can be	6
effective diagnostic method for	6
peripheral blood mononuclear cells	6
gel stained with ethidium	6
of isothermal amplification techniques	6
mthfr c t and	6
the amplified products were	6
results indicate that the	6
hais due to ics	6
and feces by reverse	6
that the detection limit	6
of the most important	6
applied for the detection	6
visual detection of isothermal	6
change from red to	6
human influenza a viruses	6
detection of chlamydia trachomatis	6
to test the specificity	6
it is important to	6
this method has been	6
has the advantage of	6
authors declare that they	6
that they have no	6
positive for h n	6
fold more sensitive than	6
indicating that the rt	6
the present study was	6
serum and urine samples	6
c g g type	6
in a biosafety level	6
tissues and feces by	6
lamp for the detection	6
recombinase polymerase amplification assay	6
in the treatment of	6
the lamp method has	6
approved by the institutional	6
lamp has been used	6
has been found to	6
on ice for min	6
of the mixture was	6
in the acute stage	6
influenza a h n	6
e gene and n	6
electrophoresis on a agarose	6
of the n gene	6
diagnosis of h n	6
of loopmediated isothermal amplification	6
due to the high	6
the e gene and	6
the detection limits of	6
the number of clinical	6
to the qpcr assay	6
designed using the primer	6
viral rna mini kit	6
detection of porcine circovirus	6
is the first report	6
of influenza a and	6
water bath or heat	6
and the reaction was	6
we would like to	6
virus rna using colorimetric	6
time polymerase chain reaction	6
to ensure that the	6
when the reaction was	6
the lamp assay is	6
has been used in	6
we developed a rapid	6
in the target dna	6
using reverse transcription loop	6
buffer and stored at	6
incubated at c for	6
the results indicated that	6
of mgcl and dntps	6
to the results of	6
can be carried out	6
amplification assay development and	6
total rna was extracted	6
the rapid diagnosis of	6
it has been reported	6
n gene of sars	6
isothermal amplifi cation method	6
this is the first	6
viral nucleic acid testing	6
the use of rt	6
the lowest detection limits	6
natural science foundation of	6
patients with pulmonary adenocarcinoma	6
sets of lamp primers	6
that the lamp assay	6
assay for the diagnosis	6
in agreement with the	6
virus genome by loop	6
compared with those of	6
used as templates for	6
eight distinct regions on	6
is shown in figure	6
detection of isothermal nucleic	6
of f fimbriae gene	6
science foundation of china	6
is thought to be	6
the cdc real time	6
the detection of various	6
for the first time	6
pcr and lamp assays	6
was used as template	6
n genes of sars	6
the cap of the	6
direct nucleic acid testing	6
was carried out using	6
a novel coronavirus from	6
primers fip and bip	6
similar to that of	6
t and aldh glu	6
the f and b	6
of the amplification reaction	6
lamp assays have been	6
as a negative control	6
and application of a	6
bath or heat block	6
of zika virus in	6
determine the sensitivity of	6
the presence of a	6
number of clinical samples	6
samples were positive for	6
article can be found	6
c for min in	6
of the severe acute	6
mediated isothermal amplification date	6
test the specificity of	6
qiaamp viral rna mini	6
was the same as	6
more sensitive than pcr	6
isothermal amplification assay rapid	6
of h avian influenza	6
lamp assay and lamp	6
rna using colorimetric lamp	6
to the reaction mixture	6
the newly developed rt	6
alternately inverted repeats of	6
assays for the detection	6
of respiratory syncytial virus	6
loopmediated isothermal amplification reaction	6
sensitivity of the assay	6
nasopharyngeal swabs and sputum	6
were collected from patients	6
the bst dna polymerase	6
the time required for	6
primer explorer v software	6
amplification method rapid detection	6
detection of viral hemorrhagic	6
virus dna by loop	6
lamp assay for covid	6
using the naked eye	6
the accuracy of the	6
the lamp reaction is	6
used for the diagnosis	6
with the use of	6
nucleic acid detection with	6
the n gene of	6
acid amplification using ph	6
rapid detection and quantification	6
influenza a viruses by	6
ethics committee of the	6
the presence of the	6
the qiaamp viral rna	6
lamp assay for sars	6
u of bst dna	6
acid sequence based amplification	6
in our previous study	6
rapid detection of newcastle	6
sybr green i dye	6
and quantifi cation of	6
the use of this	6
the lamp products were	6
the need for a	6
at r min for	6
assays for detection of	6
rna was eluted in	6
are shown in figure	6
rapid and specific detection	6
a simple water bath	6
acidification of the reaction	6
for detection of sars	6
mediated isothermal amplification loop	6
detection of human papillomavirus	6
were designed using the	6
detection of mycobacterium ulcerans	6
prevention and control of	6
regions of the target	6
at c for h	6
the authors declare that	6
has been reported that	6
cats with and without	6
negative diagnosis for covid	6
has the potential to	6
the world health organization	6
the detection of m	6
c t and aldh	6
swabs and sputum samples	6
in addition to the	6
ibv or ndv alone	5
infection by newly developed	5
in order to determine	5
mediated isothermal amplification to	5
from pharyngeal swab specimens	5
less than an hour	5
the detection of the	5
min and terminated at	5
have been developed and	5
were subjected to electrophoresis	5
the ns a gene	5
culture medium and biological	5
pcr for the detection	5
point of care diagnostic	5
patients with pneumonia in	5
there is no need	5
detect ibv and ndv	5
has been reported to	5
used for the rt	5
for quantifying template dna	5
coronavirus severe acute respiratory	5
nested polymerase chain reaction	5
of blood samples from	5
and a set of	5
through the use of	5
an isothermal amplification reaction	5
for nucleic acid testing	5
with or without loop	5
following intravenous alteplase infusion	5
be used to detect	5
than h from sample	5
ns ag by elisa	5
could be used as	5
of the virus in	5
methods for the detection	5
and a specificity of	5
was able to detect	5
lamp reaction can be	5
institute of medical sciences	5
can be observed by	5
and n genes of	5
of nucleic acids in	5
with a sensitivity of	5
an independent cohort of	5
direct detection of human	5
of the virus and	5
more sensitive than the	5
loops formed by annealing	5
annealing between alternately inverted	5
in shrimp by loop	5
step reverse transcription loop	5
the detection of herpes	5
detection method for novel	5
and validation of a	5
detection of egfr mutations	5
reactions were incubated at	5
a dilution series of	5
the terms and conditions	5
as a result of	5
detection and quantifi cation	5
amplification rapid detection of	5
detection and quantitation of	5
and conditions of the	5
the central nervous system	5
a and b by	5
of the creative commons	5
lamp products were separated	5
terms and conditions of	5
japanese encephalitis virus by	5
positive results were observed	5
from left to right	5
designed based on the	5
the detection of zikv	5
be found in the	5
studies have shown that	5
for min followed by	5
in the number of	5
the specificity of lamp	5
chikungunya virus by reverse	5
have been shown to	5
in a single step	5
to be involved in	5
or without loop primers	5
with that of rt	5
nasopharyngeal swabs from covid	5
this article is an	5
rapid diagnostic method for	5
of lamp reaction for	5
of fish and shellfish	5
each of fip and	5
were designed from the	5
of sensitivity and specificity	5
access article distributed under	5
lamp reaction was performed	5
targeting severe acute respiratory	5
from to copies per	5
n and pdm h	5
a sense sequence of	5
present in the reaction	5
lamp can be performed	5
were able to detect	5
the amplification efficiency of	5
formed by annealing between	5
used in the lamp	5
of nucleic acid testing	5
can be performed using	5
had a detection limit	5
the b c region	5
these results indicate that	5
less than h from	5
the amplification reaction was	5
does not require expensive	5
be used as an	5
of this assay for	5
the point of care	5
positive for pdm h	5
and pdm h n	5
and b by loop	5
a total of six	5
by loopmediated isothermal amplification	5
showed that the rt	5
to develop and validate	5
test for detection of	5
in a agarose gel	5
are summarized in table	5
color change from orange	5
limit of detection for	5
tris buffer was used	5
in a mouse model	5
c for s and	5
the results were compared	5
for the treatment of	5
the unfolded protein response	5
of the target dna	5
from sample to result	5
a significant number of	5
primers were designed from	5
of the amplified products	5
has been applied for	5
products were separated on	5
higher sensitivity than the	5
isolation for the detection	5
amplification of dna development	5
the color of the	5
by zhang et al	5
this study showed that	5
virus infection by newly	5
a major role in	5
forward and reverse primers	5
by electrophoresis on a	5
lowest detection limits of	5
assay for detection and	5
with pneumonia in china	5
a total of samples	5
rift valley fever virus	5
in this study were	5
has been successfully applied	5
at the point of	5
for the amplification of	5
fish and shellfish diseases	5
lamp was found to	5
at an early stage	5
more sensitive than conventional	5
with immunochromatography and virus	5
in the field of	5
the slit lamp shield	5
for hais due to	5
virus isolation for the	5
detection limit of copies	5
the n gene rt	5
determine the optimal reaction	5
amplification of rna technology	5
it can be used	5
vitro and in vivo	5
the risk of contamination	5
isothermal amplification assays targeting	5
was found in the	5
under the terms and	5
detecting ibv or ndv	5
novel coronavirus from patients	5
is no need for	5
buffer was used as	5
to the presence of	5
detection of mumps virus	5
with those of the	5
total of l of	5
avian infectious bronchitis virus	5
of a rapid and	5
the detection and quantification	5
in a color change	5
time nucleic acid sequence	5
kindly provided by dr	5
as a function of	5
method for the rapid	5
products were subjected to	5
an important role in	5
acs synthetic biology pubs	5
virus in shrimp by	5
directly from clinical samples	5
the activity of dna	5
lamp x master mix	5
of the rna standard	5
on the target gene	5
and two loop primers	5
and the cdc real	5
h n and pdm	5
the template input was	5
japanese encephalitis virus rapid	5
of nucleic acids and	5
time turbidimetry of lamp	5
time detection of chikungunya	5
detection of white spot	5
are listed in table	5
in the design of	5
using a set of	5
the objective of this	5
herpes simplex virus infection	5
was extracted using the	5
and compared with the	5
amplification to a culture	5
order to compare the	5
and typing of influenza	5
diagnosis of herpes simplex	5
from sample collection to	5
at o c for	5
pi k akt mtor	5
a report of cases	5
elution buffer and stored	5
open access article distributed	5
a culture medium and	5
effusions of cats with	5
was carried out at	5
lamp assay to detect	5
novel coronavirus severe acute	5
in order to detect	5
of human papillomavirus type	5
reaction was performed in	5
is a novel nucleic	5
dna was detected in	5
subjected to electrophoresis on	5
system for rapid diagnosis	5
assays targeting severe acute	5
lamp reaction for quantifying	5
qlamp assay detection results	5
in the online version	5
primers were designed based	5
pcr for detection of	5
of enterotoxigenic escherichia coli	5
a negative regulator of	5
the need for expensive	5
of novel coronavirus severe	5
the mean detection rates	5
were carried out at	5
the diagnostic performance of	5
for nucleic acid amplification	5
or under uv light	5
assays have also been	5
article distributed under the	5
the assay can be	5
and newcastle disease virus	5
performance of the rt	5
in the growing season	5
comparison with immunochromatography and	5
simplex virus infection by	5
the target dna and	5
the early stages of	5
between alternately inverted repeats	5
it has also been	5
of clinical samples that	5
in less than h	5
green i was added	5
lamp reaction was carried	5
detection of african trypanosomes	5
develop and validate a	5
for use in the	5
food and drug administration	5
time reverse transcription polymerase	5
is considered to be	5
in health and disease	5
ec nv detection kits	5
is able to detect	5
been shown to induce	5
the diagnostic potential of	5
by the detection of	5
the commercial qiacube extraction	5
by loop mediated isothermal	5
for min and terminated	5
in order to compare	5
that of the rt	5
lamp has also been	5
conditions of the creative	5
for detection and differentiation	5
cap of the tube	5
to determine the detection	5
encephalitis virus by real	5
the creative commons attribution	5
for the e gene	5
of the ec nv	5
immunochromatography and virus isolation	5
including two outer primers	5
multiple loops formed by	5
lamp and the prv	5
i was added to	5
the use of the	5
and the n gene	5
which can be used	5
turbidimetry of lamp reaction	5
were positive for h	5
the lowest detection limit	5
established in this study	5
orf a and the	5
the extracted rna was	5
color change from red	5
a viruses by loop	5
were determined to be	5
method rapid detection of	5
was used as nc	5
diagnosis of h avian	5
a high degree of	5
isothermal amplification to a	5
and the use of	5
orf a and orf	5
were obtained from the	5
early stage of infection	5
false positive results in	5
human respiratory disease in	5
encephalitis virus rapid and	5
infectious hematopoietic necrosis virus	5
autocycling strand displacement dna	5
was estimated to be	5
the microfluidic disc at	5
in the same strand	5
validated with clinical samples	5
r min for s	5
were separated on a	5
can be applied for	5
method for diagnosis of	5
typing of influenza a	5
of the novel rt	5
such as a thermal	5
the specificities of the	5
by the presence of	5
used to design the	5
samples isolated from pharyngeal	5
by annealing between alternately	5
rna in clinical samples	5
rapid diagnosis of herpes	5
reaction for quantifying template	5
an open access article	5
as a diagnostic tool	5
amplification as a rapid	5
amplification assays targeting severe	5
targeting the e gene	5
used for detection of	5
with a commercial rt	5
activity of dna polymerase	5
medium and biological substances	5
for detection of african	5
pdm h n and	5
it has been shown	5
article is an open	5
of autophagy in the	5
the detection of nvs	5
as a rapid early	5
pcr assay for detection	5
using a reverse transcription	5
water bath or heating	5
and two outer primers	5
pmol each of the	5
in vitro and in	5
diluted sybr green i	5
for the rapid diagnosis	5
detection of loopmediated isothermal	5
using a nondenatured template	5
the reaction tube and	5
a total of l	5
distributed under the terms	5
is an open access	5
nucleic acid amplification techniques	5
acute phase of infection	5
developed for detection of	5
assay was developed for	5
is a simple and	5
bath or heating block	5
as a thermal cycler	5
can be adapted to	5
isolated from pharyngeal swab	5
the detection limit was	5
acid detection with crispr	5
to a culture medium	5
coronavirus from patients with	5
this study was approved	5
autophagy is involved in	5
samples that were negative	4
with the prv ge	4
and validate a novel	4
the inconsistent samples between	4
loopmediated isothermal amplification as	4
a primer set for	4
was more than copies	4
the recent outbreak of	4
in supplementary table s	4
other respiratory viral infections	4
majority of samples with	4
a total of blood	4
by means of the	4
was higher than that	4
of the positive reaction	4
h n influenza virus	4
rapid detection of viral	4
the authors would like	4
rapid detection of covid	4
amplification of dna in	4
during intravenous alteplase infusion	4
a nondenatured template accelerated	4
the color change can	4
compared with those obtained	4
dao thi et al	4
detection rate of the	4
standards ranging from to	4
detected vhs rna at	4
one microliter of sybr	4
concentrations of mgcl and	4
the reliability of the	4
the gi primer set	4
the details of the	4
reaction using a nondenatured	4
prior to rna isolation	4
enzyme linked immunosorbent assay	4
ex taq tm ii	4
were grouped into clades	4
directly under daylight or	4
detection of nvs in	4
have been proposed for	4
precipitate of magnesium pyrophosphate	4
in the pathogenesis of	4
tested for the presence	4
with ethidium bromide and	4
it does not require	4
premix ex taq tm	4
and found to be	4
the sensitivity of detection	4
emergency of international concern	4
detection of norovirus from	4
hnb and genefinder tm	4
of the base apparatus	4
plasmid standards of pmd	4
min from sample collection	4
eukaryotic initiation factor e	4
loopmediated isothermal amplification using	4
a reverse transcriptional loop	4
the products of the	4
detection of porcine epidemic	4
mumps virus genome by	4
does not require a	4
isothermal amplification reaction using	4
negative for ns ag	4
within each sample type	4
the sensitivity of this	4
c g a type	4
these two methods were	4
that of nested rt	4
of proliferative kidney disease	4
as well as a	4
of the viral genome	4
eliminates the need for	4
diagnosis of feline infectious	4
valuable tool for the	4
samples can be used	4
amplification reaction using a	4
the two assays was	4
is an isothermal amplification	4
there is an urgent	4
was selected as the	4
and sensitivity of the	4
primer set targeting the	4
and an asymptomatic carrier	4
using a reverse transcriptional	4
the virus can be	4
is complementary to the	4
of human genomic dna	4
as well as for	4
the efficacy and safety	4
of false positive results	4
four sets of lamp	4
hepatitis c virus infection	4
with human respiratory disease	4
in fish and shellfish	4
the colorimetric lamp assay	4
to terminate the reaction	4
supported by the national	4
of dna development and	4
products were electrophoresed on	4
at a final concentration	4
which is suitable for	4
sensitivity and specificity for	4
amplified products were analyzed	4
assay does not require	4
myopathy with excessive autophagy	4
performed as described previously	4
zoster virus infection by	4
diagnostic method for detection	4
hepatitis b virus dna	4
of the assay was	4
of a complementary sequence	4
a novel dna amplification	4
a template for rt	4
equivalent to that of	4
gel electrophoresis and hnb	4
the detection sensitivity of	4
the early diagnosis of	4
amplified dna products from	4
sybr green i based	4
temperature of z c	4
the ability to detect	4
cyp c and cyp	4
of porcine epidemic diarrhea	4
a higher positive rate	4
was added to each	4
rna extraction was performed	4
inhibit and activate autophagy	4
between f and b	4
the epidermal growth factor	4
influenza virus infection by	4
the fl uorescent dye	4
lateral flow assay for	4
dye sybr green i	4
of polymerase chain reaction	4
simulated chemical reaction to	4
commercial isothermal amplification kit	4
detection of the severe	4
designed to accelerate the	4
both autophagy and apoptosis	4
many bands of different	4
was determined to be	4
amplification of dna real	4
is less sensitive than	4
from orange to green	4
specificities of the assays	4
complementary sequence of f	4
the reaction time of	4
high pure rna tissue	4
as few as copies	4
between these two methods	4
for detection of middle	4
the f c region	4
in patients with acute	4
lamp reactions can be	4
just before the rna	4
authors would like to	4
japanese encephalitis virus infection	4
to the standard curve	4
of infectious bronchitis virus	4
newly developed influenza h	4
preparation to the result	4
development of rapid and	4
of each sample was	4
most widely used method	4
white precipitate of magnesium	4
in a pcr cycler	4
was found to have	4
min to terminate the	4
lessons from the coronavirus	4
and specificity of a	4
internal carotid artery stenosis	4
the ns gene of	4
template input was more	4
they have no competing	4
in less than an	4
in a final volume	4
the sensitivity of hpv	4
of amv reverse transcriptase	4
daylight or under uv	4
as low as copies	4
a simple method for	4
increase the sensitivity of	4
transcription recombinase polymerase amplification	4
were performed in a	4
application of reverse transcription	4
mean detection rates of	4
of mumps virus genome	4
of the presence of	4
could be used to	4
dna was eluted in	4
from the coronavirus disease	4
a broad range of	4
of lamp primers were	4
and quantification of japanese	4
primers that recognize six	4
dna amplification and detection	4
the onset of illness	4
important lessons from the	4
detection of influenza viruses	4
between positive and negative	4
of norovirus from fecal	4
specificity of the lamp	4
as part of the	4
the fluorescence signal of	4
without rna extraction for	4
for laboratory diagnosis of	4
for influenza a and	4
after the addition of	4
influenza h hemagglutinin gene	4
primers were designed by	4
of a novel coronavirus	4
are presented in table	4
and a sense sequence	4
development of a rapid	4
detection of salmonella enterica	4
phase of nasba process	4
assay was able to	4
the value of lamp	4
final reaction volume of	4
recognize a total of	4
by the lamp method	4
and simple detection of	4
detection and serotyping of	4
incubation at room temperature	4
can serve as a	4
four to six primers	4
combined with lateral flow	4
ll of the lamp	4
in the diagnosis of	4
has the advantages of	4
reaction by loopmediated isothermal	4
preprint is the author	4
results of the lamp	4
mediated isothermal amplification the	4
the detection of porcine	4
of the cyp c	4
such as human immunodeficiency	4
diagnosis of pandemic influenza	4
the crispr cas a	4
regulated optical detection unit	4
pure rna tissue kit	4
as described in the	4
respiratory disease in china	4
respiratory syndrome coronavirus loop	4
is inhibited by rapamycin	4
well as in the	4
c and cyp c	4
mgcl and dntps were	4
development of a novel	4
the use of loop	4
as the positive control	4
of the target gene	4
target dna and initiates	4
a single and two	4
a reaction time of	4
we have adapted a	4
diagnostic potential of rt	4
a mixture of stem	4
in the human genome	4
primers were designed targeting	4
medrxiv preprint is the	4
the detection of mycobacterium	4
blood samples from pregnant	4
false positives due to	4
virus in genital lesions	4
is an urgent need	4
that were negative for	4
developed influenza h hemagglutinin	4
have adapted a simulated	4
products were visualized by	4
coronavirus associated with human	4
five lamp primer sets	4
less sensitive than the	4
has been shown that	4
with a total of	4
shown in figure a	4
the e region of	4
of our study was	4
and backward loop primer	4
samples were stored at	4
lamp assay with the	4
consent was obtained from	4
this integrated isothermal device	4
and other respiratory viruses	4
chinese center for disease	4
rna extraction followed by	4
rna can be detected	4
new coronavirus associated with	4
the course of the	4
the conserved region of	4
the positive reaction was	4
a lamp assay for	4
colorimetric lamp master mix	4
a valuable tool for	4
positive according to the	4
designed primers that recognize	4
consists of a complementary	4
f f f f	4
with conventional snp detection	4
molecular test for the	4
and points of entry	4
wild type and homozygous	4
that of the ec	4
to be seen whether	4
the ec nv detection	4
release of nucleic acids	4
real time nucleic acid	4
type and homozygous mutation	4
to the detection of	4
the clinical application of	4
for ns ag by	4
the detection of human	4
and a dna polymerase	4
the orf a gene	4
mediated isothermal amplification and	4
of west nile and	4
influenza a viruses and	4
was developed in this	4
have been found to	4
on the surface of	4
and the sensitivity of	4
written informed consent was	4
preceding stroke treatment because	4
accelerated reaction by loopmediated	4
total rna extracted from	4
fully automated centrifugal microfluidic	4
there was no amplification	4
the development of an	4
of dna rapid detection	4
fecal specimens by real	4
heated at c for	4
when detecting ibv or	4
samples from respiratory secretions	4
have no competing interests	4
denv ns serotype specific	4
linked myopathy with excessive	4
a ct cutoff of	4
of the ns gene	4
selected as the target	4
were designed to accelerate	4
complementary to the f	4
was transferred to a	4
the chinese center for	4
times higher than that	4
remains to be seen	4
which is inhibited by	4
simplex virus in genital	4
structures with multiple loops	4
to compare the sensitivity	4
a novel method of	4
x hinc ii digest	4
assays have been developed	4
lamp primer set a	4
has been demonstrated to	4
of the ns serotype	4
diagnosis and treatment of	4
it is difficult to	4
bst dna polymerase large	4
in the control group	4
was used to compare	4
in order to facilitate	4
rapid diagnosis of sars	4
such as nucleic acid	4
primers were designed using	4
assay to detect sars	4
plays important roles in	4
detection of sry gene	4
amplification combined with a	4
that can be used	4
no conflicts of interest	4
copies of the target	4
been developed for the	4
samples were subjected to	4
for the lamp assay	4
viral nucleic acids in	4
a and the n	4
respiratory syndrome coronavirus development	4
the development of lamp	4
of potato leafroll virus	4
loop dnas with various	4
cycles of denaturation at	4
the n gene was	4
under daylight or under	4
by cycles of denaturation	4
nicking and extension amplification	4
lamp and is pcr	4
to an increase in	4
dependent rna polymerase gene	4
the optimal temperature for	4
stages of the disease	4
the integrated isothermal device	4
for detection of mumps	4
a complementary sequence of	4
in a clinical setting	4
for lamp and pcr	4
detection of lamp products	4
center for disease control	4
with a variety of	4
the reaction mixture and	4
change from orange to	4
included in the study	4
patients with other respiratory	4
detection of potato leafroll	4
acute respiratory syndrome virus	4
with postweaning multisystemic wasting	4
of pacific white shrimp	4
the loop mediated isothermal	4
in the care home	4
the majority of samples	4
for min to terminate	4
of and important lessons	4
assay can be applied	4
the amount of dna	4
and accurate diagnosis of	4
coronavirus using reverse transcription	4
and was stored at	4
a simulated chemical reaction	4
in terms of sensitivity	4
highly sensitive and specific	4
microliter of sybr green	4
observed by the naked	4
total volume of ml	4
primers used in this	4
samples were obtained from	4
serial dilution of the	4
that the detection rate	4
and incubated for min	4
loop mediated isothermal amplificatio	4
of the college of	4
vhs rna at a	4
for denv and denv	4
reverse transcription recombinase polymerase	4
showed higher sensitivity than	4
standard curve was generated	4
according to the who	4
reaction mixtures were incubated	4
shown to increase the	4
selected temperature for min	4
results are shown in	4
pcr and lamp target	4
combined with a lateral	4
agarose gel electrophoresis and	4
for the containment of	4
nucleic acid testing methods	4
is more sensitive than	4
temperature in less than	4
lamp assay and conventional	4
was performed on the	4
was detected in samples	4
available under a perpetuity	4
therascreen egfr pcr kit	4
a from water samples	4
were used in the	4
outer primers f and	4
min at room temperature	4
because of the rapid	4
college of life sciences	4
a sensitive and specific	4
rna was subjected to	4
during the coronavirus disease	4
complementary sequence of b	4
used for rna extraction	4
for detection of chlamydia	4
of lamp for the	4
equipment such as a	4
a function of the	4
the use of crude	4
o c for min	4
with a low viral	4
lamp assays for the	4
cyp c g a	4
committee of the college	4
aim of this study	4
sequence analysis of the	4
e and n genes	4
total of blood samples	4
specially designed primers that	4
amplification efficiency of the	4
a h n virus	4
sequencing for detecting sars	4
sensitivity of copies reaction	4
development of a real	4
before the rna extraction	4
with two different genotypes	4
the purpose of this	4
of the present study	4
compounds that inhibit and	4
national natural science foundation	4
u bst dna polymerase	4
has also been shown	4
negative by the rt	4
in the same way	4
efficacy and safety of	4
is a member of	4
dna polymerase large fragment	4
at a concentration of	4
f c and b	4
being tested for covid	4
the t p strain	4
performed in a total	4
sensitivity of the ns	4
isothermal amplifi cation assay	4
loopmediated isothermal amplification for	4
that is complementary to	4
of primers and kcl	4
emergent carotid artery stenting	4
rna at a dilution	4
set targeting the n	4
forward inner primer fip	4
is in agreement with	4
were obtained from patients	4
dna was extracted by	4
by genskey medical technology	4
mediated isothermal amplification on	4
diagnostic test for bu	4
test for the novel	4
promote cell cycle progression	4
version of this article	4
strains were grouped into	4
nucleic acid amplification assay	4
clinical application of the	4
herpes simplex virus in	4
were negative for ns	4
of viral rna in	4
in the initial stage	4
detection of the sars	4
study is the first	4
the acute stage of	4
amplification test for detection	4
reactions were performed in	4
were designed according to	4
and does not require	4
lamp reactions were performed	4
to reach the threshold	4
analysis of clinical samples	4
diagnosis of sickle cell	4
detected as positive by	4
set of four specially	4
rapid detection of varicella	4
the overall sensitivity of	4
the need for rna	4
for influenza a viruses	4
of rna extracted from	4
emerging viruses of biomedical	4
and a final extension	4
of acute gastroenteritis in	4
determine the detection limit	4
and important lessons from	4
coronavirus by a loop	4
the aim of this	4
detection of chik virus	4
diagnostic performance of rt	4
detection of infectious bronchitis	4
for h n virus	4
assays for rapid detection	4
mediated isothermal amplification test	4
of the primers are	4
complementary to the b	4
included in each run	4
color change can be	4
quantification of japanese encephalitis	4
mixtures were heated at	4
detection of loop mediated	4
of isothermal master mix	4
the etiological agent of	4
syndrome coronavirus using reverse	4
and lamp target sequences	4
of the assay to	4
such as the requirement	4
application of a rapid	4
with the help of	4
the spread of disease	4
rapid screening method for	4
use of lamp for	4
lamp was performed at	4
detection of hcv rna	4
of care diagnostic test	4
was used to determine	4
has also been reported	4
water bath or a	4
nondenatured template accelerated reaction	4
of elution buffer and	4
detection of viral rna	4
lamp assay could be	4
in the reaction tubes	4
amplification of dna with	4
has proven to be	4
were synthesized by invitrogen	4
be due to the	4
heating and visual inspection	4
and thus can be	4
and bip primers at	4
the qpcr detection results	4
that were used for	4
had a similar sensitivity	4
with multiple loops formed	4
lamp assay does not	4
were included in the	4
can be performed in	4
as human immunodeficiency virus	4
of autophagic vacuoles and	4
the college of life	4
sensitivity of this rt	4
in the use of	4
outbreaks of acute gastroenteritis	4
in the context of	4
specifically designed to recognize	4
viruses of biomedical importance	4
to induce autophagy and	4
days after the onset	4
concentrations of primers and	4
to electrophoresis on a	4
by newly developed influenza	4
a new coronavirus associated	4
dna products from the	4
amplification assays for detection	4
sample nucleic acid releasing	4
the reaction mixtures were	4
been used in the	4
the ap pathway is	4
mediated isothermal amplification with	4
the pi k akt	4
primers targeting the n	4
the bottom of the	4
yielding inconsistent results between	4
such as sybr green	4
enzymatic amplification of dna	4
the n gene and	4
it remains to be	4
the lamp method may	4
gene was found to	4
acute respiratory distress syndrome	4
of gene copy number	4
techniques have been developed	4
rapid detection of norovirus	4
patients suspected clinically of	4
for the novel coronavirus	4
simple and sensitive rt	4
precautions to prevent cross	4
by a novel dna	4
the reaction was terminated	4
objective of this study	4
a disposable pocket warmer	4
specificity and sensitivity in	4
informed consent was obtained	4
template accelerated reaction by	4
assay could be used	4
input was more than	4
is involved in the	4
nucleic acid releasing agent	4
and differentiation of the	4
a stepone tm system	4
in the formation of	4
been developed to detect	4
from fecal specimens by	4
respiratory syndrome coronavirus using	4
ensure that the primers	4
collected from patients with	4
of mycobacterium tuberculosis in	4
backward inner primer bip	4
ibv and ndv rna	4
the gold standard for	4
the detection of nucleic	4
distribute in all clades	4
the robustness of the	4
in enterotoxigenic escherichia coli	4
because of its simplicity	4
the ns serotype specific	4
at the same time	4
lamp assay may be	4
novel dna amplification method	4
with neurodegeneration in adulthood	4
for molecular detection of	4
detection of hepatitis c	4
of swine transmissible gastroenteritis	4
agarose gel stained with	4
extraction followed by rt	4
can also be used	4
the optimal reaction condition	4
is a negative regulator	4
we developed a visual	4
concentration of the positive	4
extracted rna was subjected	4
positive for influenza a	4
its ability to amplify	4
that the assay is	4
a loopmediated isothermal amplification	4
isolation and identification of	4
and patients with other	4
developed by notomi et	4
lamp products were electrophoresed	4
mediated amplification of rna	4
of white spot syndrome	4
to the use of	4
rapid and simple detection	4
samples yielding inconsistent results	4
on agarose gel electrophoresis	4
swabs in amies medium	4
shown in table iv	4
dna polymerase used in	4
specificity of the reaction	4
lamp assay was a	4
the development of the	4
the francis crick institute	4
of influenza a virus	4
polymerase activity of the	4
syndrome coronavirus development of	4
dilutions of the rna	4
after magnetic bead rna	4
associated with human respiratory	4
samples were collected in	4
has great potential for	4
the detection of tomato	4
study was supported by	4
rna from clinical samples	4
and virus isolation for	4
autophagy has been shown	4
samples from pregnant women	4
is used as a	4
in the acute phase	4
patients and an asymptomatic	4
method for novel sars	4
norovirus from fecal specimens	4
that inhibit and activate	4
by the ccc pipeline	4
were confirmed to be	4
the advantages of this	4
and porcine reproductive and	4
used as a reference	4
rna extraction and rt	4
answer detection of viral	4
testing with patient samples	4
with other respiratory viruses	4
also been reported that	4
characteristics of and important	4
monitoring of the lamp	4
with a lateral flow	4
performed as described in	4
are provided in table	4
for min at room	4
the detection and differentiation	4
reaction by loop mediated	4
hybridization chain reaction for	4
for the reported sample	4
herpesvirus dna by loop	3
using loop primers rapid	3
fold after either qiacube	3
for reporting diagnostic accuracy	3
and the study was	3
a new coronavirus of	3
various investigators for rapid	3
the ability of the	3
amplifi cation method for	3
either qiacube or magnetic	3
a large number of	3
needs to be validated	3
of the orf ab	3
the potential of lamp	3
study demonstrates that the	3
to prevent the spread	3
was less sensitive and	3
of the established rt	3
isothermal amplification microdevice for	3
in both the rt	3
under the optimized conditions	3
public domain and hospitals	3
where the fluorescence signal	3
target sequencing for accurate	3
shown to be a	3
growth factor receptor mutations	3
reaction and a rapid	3
for diagnosis of pandemic	3
linked immunosorbent assay for	3
lamp does not require	3
the mixture was taken	3
these primers were then	3
between both assays was	3
the specificity of amplification	3
specially designed set of	3
performance of the assays	3
time reverse transcriptase polymerase	3
and it is also	3
potentially be a valuable	3
sweet spot for molecular	3
and subtyping of influenza	3
sensitivity than conventional rt	3
were used to detect	3
detection of noroviruses in	3
not receive any specific	3
be a suitable diagnostic	3
amplification and detection in	3
be an effective treatment	3
out in a total	3
typing of emerging viruses	3
the toxic dinoflagellate alexandrium	3
water prior to rna	3
z c to z	3
blood samples were collected	3
lamp can be used	3
the protocol established in	3
relatively small sample size	3
clinically of denv infection	3
qpcr was performed using	3
clinical specimens were used	3
operating procedures for sars	3
human herpesvirus dna by	3
bottom of the tube	3
and quantitative detection of	3
rna extracted from yhv	3
demonstrated the use of	3
according to the results	3
be observed by the	3
the ml lamp products	3
a rapid and accurate	3
used as the positive	3
the identification of the	3
in less than one	3
with a blast search	3
assay is highly specific	3
sequence of the vhs	3
the detection of several	3
the impact of the	3
accurate and comprehensive detection	3
was a linear correlation	3
by heat inactivation at	3
amplifi cation can also	3
primers were used to	3
in is the author	3
ibv and ndv simultaneously	3
in order to avoid	3
epidemiology of novel coronavirus	3
detection of murine norovirus	3
than that of pcr	3
specific primers were designed	3
for molecular diagnosis of	3
ag by elisa and	3
that was developed in	3
been infected with wild	3
qiacube or magnetic bead	3
we developed a simple	3
a new method for	3
using primer explorer version	3
its mechanism of action	3
out under isothermal conditions	3
were used as the	3
the results indicated the	3
with other respiratory viral	3
sensitivity than the rt	3
on the use of	3
that the lamp method	3
the ct values for	3
at a temperature of	3
herpesvirus dna in serum	3
of the practical limitations	3
ranging from to iu	3
fold dilution series up	3
may serve as a	3
specific to the target	3
detection of escherichia coli	3
too expensive to be	3
pmol of each primer	3
assay is based on	3
of the samples with	3
of cases from the	3
positive rate than sputum	3
sequences under isothermal conditions	3
described by notomi et	3
serves as a template	3
with ibv and ndv	3
end of the viral	3
formation accelerated reaction by	3
the amplification of dna	3
dna in the reaction	3
in the writing of	3
nucleic acids in complex	3
used as a positive	3
loop primers rapid detection	3
of pathogens via the	3
isothermal amplification test for	3
applied both protocols on	3
the lamp reaction for	3
monitoring the progress of	3
in vitro antiviral activities	3
electrophoresed on a agarose	3
and an independent cohort	3
at eight weeks of	3
dna was extracted from	3
care diagnostic test for	3
in ml buffer and	3
an intercalating agent such	3
amplification reaction for the	3
a large amount of	3
and then aligned to	3
have been used to	3
detection of swine transmissible	3
diagnosis of bordetella pertussis	3
have been developed in	3
ph changes during the	3
of human herpesvirus infection	3
assay was performed in	3
for sample inactivation and	3
this study is a	3
by cycles of c	3
result diagnosis in min	3
qpcr detection of the	3
using reverse transcription rt	3
of the number of	3
as in the field	3
of childhood with neurodegeneration	3
than that obtained using	3
during the course of	3
of the detection system	3
definitive diagnosis of fip	3
has a high sensitivity	3
developed a visual and	3
probes based on the	3
the detection of clinical	3
been applied to detect	3
of zika virus by	3
a range of temperatures	3
performed in the thermalcycler	3
and direct detection of	3
ebola virus disease in	3
was chosen as the	3
the agarose gel electrophoretic	3
and without inner set	3
development of personalized medicine	3
kit for the detection	3
serotypes of denv in	3
samples positive for influenza	3
to the reaction mix	3
cation can also be	3
in order to remove	3
the commercial isothermal amplification	3
amplification products in the	3
a simple and sensitive	3
the optimal reaction time	3
positive and negative predictive	3
and fine needle aspirates	3
and typing of emerging	3
of warmstart rtx reverse	3
by the institutional review	3
agarose gel electrophoresis was	3
temperature heating and visual	3
study was designed to	3
for lamp primer design	3
the primers were selected	3
which is essential for	3
in complex biological fluids	3
terminated by heating at	3
of the copper plates	3
rna polymerase promoter sequence	3
in the viral genome	3
data associated with this	3
of the lamp technique	3
has two major components	3
the online version of	3
because of the practical	3
u of amv reverse	3
can be visualized by	3
cdc real time pcr	3
outbreak associated with a	3
blood sample and saliva	3
extraction protocol with the	3
had previously been tested	3
gastroenteritis coronavirus using loop	3
lamp method can be	3
used to detect the	3
assay was run at	3
simple water bath or	3
of mycobacterium tuberculosis and	3
revealed a negative diagnosis	3
for cyp c and	3
assays targeting gene n	3
lamp had a detection	3
detection of f fimbriae	3
copy number of each	3
was used in the	3
partial hbv polymerase gene	3
and increase the unfolded	3
of high sensitivity and	3
the same way as	3
t m for each	3
for virus origins and	3
vaccinated with prv ge	3
on a uv transilluminator	3
primers comprising two outer	3
both ibv and ndv	3
polymerase along with dntps	3
environment of the lysosome	3
was carried out for	3
detected by agarose gel	3
rna isolation in a	3
ml distilled water and	3
amplifi cation methods have	3
induce endoplasmic reticulum stress	3
with various stem lengths	3
of yellow head virus	3
the clinical samples was	3
flocked swabs in amies	3
for clinical diagnosis of	3
rapid detection of foot	3
the form of a	3
purified rna was added	3
amplification product was detected	3
to be highly specific	3
zoster virus dna by	3
for the dna polymerase	3
be used to identify	3
as the requirement for	3
inlet and outlet access	3
chest ct and rt	3
studies have reported the	3
detection with crispr nucleases	3
ha gene specific rt	3
was defined as a	3
methods have been developed	3
is an rna virus	3
pairs of primers were	3
in the opiph system	3
the laboratory diagnosis of	3
were incubated at c	3
the target in the	3
of the samples were	3
of extracted template dna	3
to the target sequence	3
and the detection of	3
isothermal amplification on a	3
widely used in the	3
and interpretation of data	3
and the results of	3
mycobacterium tuberculosis in sputum	3
that of the conventional	3
and mineral oil were	3
amplification reaction was performed	3
these results indicated that	3
is present in the	3
the early phase of	3
method for reverse transcription	3
detection and identification of	3
detection method for reverse	3
volume of l of	3
buccal swab and saliva	3
with a new coronavirus	3
presence of chik virus	3
the development of personalized	3
cycles of c for	3
quantification of nucleic acids	3
diagnosis and surveillance of	3
with that of the	3
is that it does	3
is responsible for the	3
porcine parvovirus by loop	3
also been used to	3
are not suitable for	3
the concentration of pyrophosphate	3
genotype zikv sequences had	3
a heat block and	3
in research and development	3
was kindly provided by	3
cdc real time assay	3
the nucleic acid amplification	3
extracted from allantoic fluid	3
the amplification products were	3
are at high risk	3
amplification reaction and a	3
the lamp assays were	3
a negative control template	3
was thought to be	3
studies are needed to	3
can also be accomplished	3
compared to conventional pcr	3
were eligible for inclusion	3
for detection of infectious	3
whether it is possible	3
improve the sensitivity of	3
strain was retrieved from	3
based methods require either	3
for research and diagnostic	3
lamp reactions were carried	3
tool for the detection	3
visualized directly under daylight	3
as well as lamp	3
for lamp can be	3
the detection of nv	3
amplification time of min	3
using a water bath	3
genome quantity and reaction	3
orf a and n	3
amplification products were detected	3
region of the y	3
a sensitivity of and	3
copies per reaction and	3
regarding the primer names	3
the primer sets of	3
stage of the disease	3
can be an effective	3
serum level of hbv	3
had no role in	3
the hybridization chain reaction	3
of units ml bst	3
and sensitivity with guanidine	3
lamp reaction mixtures were	3
is a strong risk	3
of patient gargle lavage	3
of the turbidity changes	3
min and then heated	3
of the e gene	3
lamp assay was validated	3
was developed for detection	3
for ibv and ndv	3
the use of multiple	3
lamp assay as a	3
the sequence of the	3
up to fold after	3
is labeled with bodipy	3
study was to establish	3
rna particles loaded with	3
diagnosis of human herpesvirus	3
pmol each of ib	3
iron accumulation in the	3
and real time rt	3
the s protein is	3
according to the protocol	3
report of cases from	3
tertiary care hospital in	3
of the opiph system	3
information regarding the primer	3
of the ml lamp	3
by using the qiaamp	3
health emergency of international	3
armored rna particles with	3
from patients infected with	3
the designed primer sets	3
the current state of	3
in the areas of	3
can detect as few	3
small cell lung cancer	3
and robust detection of	3
by various investigators for	3
initiated dna synthesis and	3
positive by the rt	3
approved the final draft	3
ct values for ic	3
chip release of viral	3
have several intrinsic disadvantages	3
comparing the results between	3
sensitivity enzymatic reporter unlocking	3
at the optimal temperature	3
declare no conflicts of	3
lamp protocol for cyp	3
study is a valuable	3
magnetic bead rna extractions	3
total of clinical samples	3
and clinical application of	3
using the online software	3
national science and technology	3
of lamp reaction volume	3
to induce endoplasmic reticulum	3
that were negative by	3
are depicted in figure	3
the target dna sequences	3
have been validated with	3
qpcr assay detection results	3
characterization of aichi viruses	3
and hepatitis b virus	3
by a specially designed	3
online version of this	3
decided to work with	3
sequences on the target	3
nucleic acid amplification testing	3
reports have indicated that	3
from patients with influenza	3
from schima crenata korth	3
and negative samples were	3
implication of multiple shedding	3
total of six distinct	3
qpcr samples also scored	3
adding sybr green i	3
rapid detection of mycobacterium	3
for diagnosis of sickle	3
african genotype zikv sequences	3
of chikungunya virus in	3
is a kind of	3
centers for disease control	3
of the f region	3
using sybr green i	3
to assess the specificity	3
has also been successful	3
lamp assay with a	3
of clinical specimens were	3
which was diluted in	3
sample with two different	3
a rapid screening method	3
both protocols on one	3
appropriate antiplatelet medication and	3
utility of this assay	3
infected with novel coronavirus	3
maps and institutional affiliations	3
used by various investigators	3
lamp has the potential	3
research and diagnostic microbiology	3
lavage samples directly added	3
at uc for min	3
strand displacing dna polymerase	3
considered the gold standard	3
nature remains neutral with	3
there was a linear	3
a temperature of z	3
human herpesvirus dna in	3
determined by measuring the	3
the authors developed a	3
inhibit the activity of	3
or elaborate methods for	3
porcine parvovirus and porcine	3
investigators for rapid detection	3
been identified as a	3
change from purple to	3
a public health emergency	3
to fold after either	3
a useful tool for	3
a linear correlation between	3
expensive equipment such as	3
than selected temperature for	3
inner primers fip and	3
rapid detection of herpes	3
color change from purple	3
reactions were performed using	3
a loop mediated isothermal	3
a final reaction volume	3
of probable bat origin	3
min after the start	3
using a stepone tm	3
linear correlation between the	3
which was times more	3
compared to that of	3
was provided by the	3
of influenza a h	3
detection using an isothermal	3
for the prevention and	3
the number of rna	3
compared with the therascreen	3
sense sequence of b	3
between the genome quantity	3
obtained using a stepone	3
sequences in the initial	3
resulted in a color	3
to evaluate the clinical	3
it shows that the	3
accelerate the lamp reaction	3
from a cohort of	3
the high specificity of	3
in this study has	3
viral rna was detected	3
were collected from suspected	3
qpcr using the e	3
recent progress in research	3
swabs were collected from	3
the presence of pcr	3
be used for rapid	3
colon cancer cells against	3
asian and african genotype	3
of the lamp assays	3
displacement synthesis primed by	3
to determine if the	3
for false positive results	3
is the most widely	3
seven clinical serum specimens	3
and serotyping of denv	3
that autophagy has a	3
not currently commercially available	3
caused by the sars	3